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The wide gap between the demand and supply of edible mustard oil can be overcome to a certain extent by enhancing the oil-recovery during mechanical oil expression. It has been reported that microwave (MW) pre-treatment of mustard seeds can have a positive effect on the availability of mechanically expressible oil. Hyperspectral imaging (HSI) was used to understand the change in spatial spread of oil in the microwave (MW) treated seeds with bed thickness and time of exposure as variables, using visible near-infrared (Vis-NIR, 400-1000 nm) and short-wave infrared (SWIR, 1000-1700 nm) systems. The spectral data was analysed using chemometric techniques such as partial least square discriminant analysis (PLS-DA) and regression (PLSR) to develop prediction models. The PLS-DA model demonstrated a strong capability to classify the mustard seeds subjected to different MW pre-treatments from control samples with a high accuracy level of 96.6 and 99.5% for Vis-NIR and SWIR-HSI, respectively. PLSR model developed with SWIR-HSI spectral data predicted (R 2  > 0.90) the oil content and fatty acid components such as oleic acid, erucic acid, saturated fatty acids, and PUFAs closest to the results obtained by analytical techniques. However, these predictions (R 2  > 0.70) were less accurate while using the Vis-NIR spectral data., (© 2024. The Author(s).)

Many applications of ultrafast and nonlinear optical microscopy require the measurement of small differential signals over large fields-of-view. Widefield configurations drastically reduce the acquisition time; however, they suffer from the low frame rates of two-dimensional detectors, which limit the modulation frequency, making the measurement sensitive to excess laser noise. Here we introduce a self-referenced detection configuration for widefield differential imaging. Employing regions of the field of view with no differential signal as references, we cancel probe fluctuations and increase the signal-to-noise ratio by an order of magnitude reaching noise levels only a few percent above the shot noise limit. We anticipate broad applicability of our method to transient absorption, stimulated Raman scattering and photothermal-infrared microscopies.

Styrax benzoin fumes have a spiritual aspect from ancient times, magical essence like a pleasant perfume, and are employed in religious ceremonies in India. This study aims to identify the volatile compounds in S. benzoin extract, their binding affinity to the bacterial target proteins, and study the antibacterial activity of the potential extract. The compounds obtained from GC-MS analysis of S. benzoin extract were subjected to molecular docking studies against DHFR of Staphylococcus aureus, tRNA synthetase of Escherichia coli , DHPS of Mycobacterium tuberculosis . Molecular docking studies revealed that seventeen compounds out of 20 compounds exhibited higher binding affinity than co-ligand (-7.00 kcal/mol) against the Staphylococcus aureus enzyme DHFR. Consequently, the crude extracts were evaluated for antibacterial activity against S. aureus, and the acetone extract showed promising findings. S. benzoin fumes might replace synthetic room fresheners, and promising compounds could be exploited in the cosmetics industry.

Global petroleum consumption suffered drastically as lockdowns were put in place to contain the Coronavirus Disease 2019 (COVID-19). As a result, oil costs dropped, making virgin plastics more cost-effective than recycled plastics. The usage of plastic has increased as a result of lifestyle modifications, cost-based incentives, and other factors, further obscuring the issue. The utilization of personal protective equipment (PPE) during the pandemic had resulted in a significant surge in the quantity of plastic waste. The plastic packaging industry achieved a revenue milestone of US$ 909.2 billion in 2021, boosting a compound annual growth rate of 5.5 %. The escalating dependence on plastics imposed additional pressure on waste management systems, which were proven to be ineffective and insufficient in addressing the issue. This situation exacerbated the problem and contributed to environmental pollution. Globally, 40 % of plastic waste ended up in landfills, 25 % was incinerated, 16 % was recycled, and the remaining 19 % infiltrated within the environment. By investing in circular technologies like feedstock recycling and enhancing infrastructural and environmental conditions, it expected to become viable to manage plastic waste flows during such a period of crisis. Investing in valorization strategies that transform plastic waste into value-added goods, such as fuels and building materials, receives a compelling macroeconomic signal when both plastic waste and plastic demand are on the rise. A robust circular economy can be accomplished by finalising the life cycle of plastic waste. The concept of Plastic Waste Footprint (PWF) aims to assess the environmental impact of plastic products throughout their intended usage period. In the midst of the emerging challenges in waste management during and post pandemic period, this research study has been conducted to explore the challenges and strategies associated with plastic waste in the environment., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Author(s).)

Mustard oil is the most commonly adulterated edible oil, invariably with argemone oil. This study was aimed to develop a parallel plate capacitive sensor for measurement of dielectric properties of pure mustard oil, pure argemone oil and their blends (25, 50 and 75%) at five levels of varying temperature (10 to 50 °C). The effect of blend ratio and temperature on the selected dielectric properties of oil-capacitance ( C ), dielectric loss tangent ( tanδ ), dielectric constant ( ε ' ), dielectric loss factor ( ε ″ ) and electrical conductivity ( σ ) were investigated. It was observed that composition of the individual oils in terms of moisture and fatty acids influenced the physical and dielectric properties. The sensor was used to relate the dielectric properties of oil samples with blend ratio and temperature by means of statistically significant ( p  < 0.05) and robust ( R 2  > 0.8) multiple linear regression model. The effect of temperature on C and ε ' was negative, while it was otherwise for tanδ , ε ″ and σ . Increase in argemone oil content in the blends, increased the dielectric measures due to the associated changes in the physical and chemical properties. The capacitive sensor could distinctly identify mustard oil, argemone oil and its blends on the basis of dielectric properties., Competing Interests: Conflicts of interestThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© Association of Food Scientists & Technologists (India) 2022, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)

Adulteration of edible substances is a potent contemporary food safety issue. Perhaps the overt concern derives from the fact that adulterants pose serious ill effects on human health. Edible oils are one of the most adulterated food products. Perpetrators are adopting ways and means that effectively masks the presence of the adulterants from human organoleptic limits and traditional oil adulteration detection techniques. This review embodies a detailed account of chemical, biosensors, chromatography, spectroscopy, differential scanning calorimetry, non-thermal plasma, dielectric spectroscopy research carried out in the area of falsification assessment of edible oils for the past three decades and a collection of patented oil adulteration detection techniques. The detection techniques reviewed have some advantages and certain limitations, chemical tests are simple; biosensors and nuclear magnetic resonance are rapid but have a low sensitivity; chromatography and spectroscopy are highly accurate with a deterring price tag; dielectric spectroscopy is rapid can be portable and has on-line compatibility; however, the results are susceptible to variation of electric current frequency and intrinsic factors (moisture, temperature, structural composition). This review paper can be useful for scientists or for knowledge seekers eager to be abreast with edible oil adulteration detection techniques.

Onion bulbs contain outer papery dry peel which is rich with industrially important high-value compounds. Removal of dry peel/scale of onion is the common practice during bulb processing, storage and marketing. A conventional manual method of scale removal involves huge labour, drudgery and means of environmental pollution. An onion descaler machine has been therefore developed to mechanize this operation, reduce drudgery and effectively collect the dry scales for industrial use. The operating parameters of the developed machine were optimized for its best performance using RSM. During optimization study, roller speed, roller slope and hopper opening were taken as independent variables. The performance of the machine was evaluated through descaling efficiency and descaling loss. During experimentation, descaling efficiency and descaling loss were obtained in the range of 35.92-98.55% and 0.82-9.07%. The optimum performance of the machine was obtained at 450 rpm roller speed, 2% roller slope and 380 mm of the hopper opening with maximum descaling efficiency (88.29%) and minimum descaling loss (3.65%). The optimization of the onion descaler parameters and their statistical relation provided the necessary information on the combination of material-machine alignment, and design parameters for enhanced descaling efficiency., Supplementary Information: The online version contains supplementary material available at 10.1007/s13197-021-05264-5., Competing Interests: Conflict of interestAll the authors enlisted declare that there are no any conflicts of interest to disclose., (© Association of Food Scientists & Technologists (India) 2021.)

Municipal solid waste prevention represents a topical point in circular economy policies within the European Union. It is therefore paramount to assess its state of decoupling from economic activity. There is weak evidence from empirical research that Italy managed to achieve this structural change locally. Decoupling is not arising homogenously in the waste generation-income relationship in Italy over the last two decades. The heterogeneity in economic performances and waste policies could be an explanation. However, it is possible to find common patterns between decoupler against non-decoupler provinces. This paper involves panel data of 103 provinces during eighteen years (2001-2018) of Italian Provinces (NUTS3) to assess the drivers of structural changes in the income-waste relation. The innovation consists of the use of a threshold model to assess the minimal requirements of socio-economic performances to decouple economic activity to non-separated waste. The threshold point differs from the tipping point as it is always observable in the panel. Previous studies mostly estimated the latter involving panel data analysis. This often resulted in fallacious interpretation, especially due to spurious regressions. The observability is a relevant criterion, as several provinces have achieved the decoupling state. Results indicate that economic activity and tourist are relevant threshold variables in waste prevention. Both are sources of local financing; thus, it is probable that circular practices are dependent on the volume of potential expenditure rather than structural characteristics such as population density., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Considering that appearance of white button mushroom (WBM) as the trigger for registering its quality, this study was aimed at analyzing the visual cues by the application of image processing tools. While L-a-b colour space and skewness was used for estimating chromatic and morphological characteristics; onset of discolouration of WBM was predicted by hyperspectral image analysis. Undamaged (UD) and damaged (D) mushrooms were stored under refrigerated conditions (3-5 °C and 90% R h ). RGB and hyperspectral images were acquired on alternate storage days 1, 3, 5, 7 and 9. Weight loss, texture and moisture content of stored mushrooms were also recorded during the storage period. Colour changes in stored UD and D were found to be in b (21.55) and a (2399) value, respectively. Browning index in D was 83-212% higher than UD mushrooms across the storage period. Weight and firmness losses in D were higher by 65.9 and 31.4%, respectively than UD. Morphological characteristic in terms of aspect ratio and roundness were not found to vary significantly over the storage period for both UD and D mushrooms. Chemometrics revealed that multiplicative scatter correction was the best pre-processing tool and that onset on discolouration is conspicuous in the spectral region of 520-800 nm. k-NN fared better than PLS-DA for correct classification (100%) of UD and D mushrooms., Competing Interests: Conflict of interestThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© Association of Food Scientists & Technologists (India) 2021.)

The present research study has evaluated the roles of different naturally occurring compounds in termite mounds of Odontotermes longignathus (GenBank Id: MZ542727.1) which facilitate to promote higher population growth of termites and subsequent biodegradation. The study has also monitored the change in physicochemical parameters along with the trend of biodegradation of complex organic carbon-based compounds like lignin, polysaccharides etc. and nitrogenous compounds from two different types of termite mounds such as developing (T1) and developed (T2) mounds. The GC MS profiling of mound samples have revealed the occurrence of different humic acids like organic materials in both T1 and T2 mound samples. Both the termite mounds have demonstrated a high population density as T1 (23.67 ± 1.56) individuals and T2 (43.51 ± 2.36) individuals per 0.1 kg of mound materials. Such observations have prompted to undertake molecular docking experiments which revealed that different molecules interact at low binding affinity with hormone receptors involved in moulting, spermatogenesis and oogenesis of termite like Adamantane carboxylate (EcR: -7.6 Kcal/mol; BTB-KLHL10: -6.2 Kcal/mol; USP-LBD: -7.3 Kcal/mol; VgR: -6.8 Kcal/mol), Benzene dicarboxylic acid (EcR: -5.5 Kcal/mol; BTB-KLHL10: -5.1 Kcal/mol; USP-LBD: -5.4 Kcal/mol; VgR: -5.6 Kcal/mol), Hexadecanol (EcR: -6.0 Kcal/mol; BTB-KLHL10: -4.4 Kcal/mol; USP-LBD: -6.9 Kcal/mol; VgR: -6.0 Kcal/mol), oxirane (EcR: -5.3 Kcal/mol; BTB-KLHL10: -4.9 Kcal/mol; USP-LBD: -5.2 Kcal/mol; VgR: -5.3 Kcal/mol) and tocopherol (EcR: -8.0 Kcal/mol; BTB-KLHL10: -5.4 Kcal/mol; USP-LBD: -7.6 Kcal/mol; VgR: -7.0 Kcal/mol). Such spontaneous ligand binding phenomenon coupled with high population density of termites have established the significance of different bioactive xenobiotics in achieving high reproductive potential of termites which in turn facilitate the process of biodegradation and enhance the nutrient enrichment in the soils of tropical deciduous forest., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)

Two-dimensional (2D) materials such as graphene, transition metal dichalcogenides (TMDs), and their heterojunctions are prospective materials for future electronics, optoelectronics, and quantum technologies. Assembling different 2D layers offers unique ways to control optical, electrical, thermal, magnetic, and topological phenomena. Controlled fabrications of electronic grade 2D heterojunctions are of paramount importance. Here, we enlist novel and scalable strategies to fabricate 2D vertical and lateral heterojunctions, consisting of semiconductors, metals, and/or semimetals. Critical issues that need to be addressed are the device-to-device variations, reliability, stability, and performances of 2D heterostructures in electronic and optoelectronic applications. Also, stacking order-dependent formation of moiré excitons in 2D heterostructures are emerging with exotic physics and new opportunities. Furthermore, the realization of 2D heterojunction-based novel devices, including excitonic and valleytronic transistors, demands more extensive research efforts for real-world applications. We also outline emergent phenomena in 2D heterojunctions central to nanoelectronics, optoelectronics, spintronics, and energy applications., Competing Interests: The authors declare no competing interests., (© 2022 The Author(s).)

Wastewater imposes a great threat to any ecosystem across the world, especially the aquatic one because of the different anthropogenic activities of human beings. The present study emphasizes the optimization of ecological parameters [pH, time (h) and temperature (°C)] employing Box-Behnken design (BBD) to achieve better bio-adsorption of a selected heavy metal [lead (Pb II)] from the wastewater through an extracellular polymeric substance (EPS) of a benthic fungus, Aspergillus penicillioides (F12) (MN210327). The relevant statistical analysis (ANOVA) has enabled to record of the optimized bio-adsorption (73.14 %) of lead (Pb II) by fungal EPS at pH (8.85) and temperature (32 °C) for a duration of 5.74 h. Besides that, at the concentration of 0.5 mg/L of EPS, the flocculating rate was noted to be highest (88.4 %) in kaolin clay and the 50 % emulsifying activity. This investigation has also opened up new vistas on the possibility of the development of an alternative method of eco-sustainable bioremediation of heavy metals by fungal EPS on an industrial scale., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Purpose: Main insight of the study was to evaluate the potential of Perionyx excavatus , an indigenous earthworm of India towards bioconversion of toxic paper mill wastes. The study had considered a comprehensive study over succession of different enzymes and microbial biomasses alongside removal of heavy metals to reflect the qualitative enrichment of the toxic waste through valorisation procedure., Methods: The process of vermicomposting was performed using ratio of paper mill sludge: cow dung: straw as 5:4:1 and Perionyx excavatus for a period of 60 days. Qualitative enrichment was considered through detailed evaluation of physicochemical parameters and succession of 19 enzymes using API ZYM technology. Heavy metal loads in terms of Zn, Cr, Cu, Pb along with bioaccumulation factor (BAF) were considered to establish functional role of earthworm in bioremediation. Microbial succession was measured in terms of PLFA biomarkers., Results: Enzymatic enrichment of different isozymes of esterase, aminopeptidase and glycosyl hydrolase were found in the vermicompost. A significant reduction of heavy metals was noticed in vermicomposting of primary sludge as Zn > Pb > Cr > Cu and secondary sludge as Zn > Pb > Cu > Cr. An increase in density of Gram positive bacterial and fungal populations was found at the end phase of vermicomposting process which supports maturity of vermicompost., Conclusion: Presence of different enzyme activities, reduction of heavy metal loads along with rise of eukaryotic PLFA markers, had illustrated the efficiency of vermicomposting as a valorisation procedure in the qualitative enrichment of toxic organic paper mill sludge., Competing Interests: Conflict of interestAuthors have no conflict of interest., (© Springer Nature Switzerland AG 2021.)

Aflatoxin-B1 contamination in maize is a major food safety issue across the world. Conventional detection technique of toxins requires highly skilled technicians and is time-consuming. Application of appropriate chemometrics along with hyperspectral imaging (HSI) can identify aflatoxin-B1 infected maize kernels. Present study was undertaken to classify 240 maize kernels inoculated with six different concentrations (25, 40, 70, 200, 300 and 500 ppb) of aflatoxin-B1 by using Vis-NIR HSI. The reflectance spectral data were pre-processed (multiplicative scatter correction (MSC), standard normal variate (SNV), Savitsky-Golay smoothing and their combinations) and classified using partial least square discriminant analysis (PLS-DA) and k-nearest neighbour (k-NN). PLS model was also developed to predict the concentration of aflatoxin-B1in naturally contaminated maize kernels inoculated with Aspergillus flavus . The potential wavelength (508 nm) was selected based on principal component analysis (PCA) loadings to distinguish between sterile and infected maize kernels. PCA score plots revealed a distinct separation of low contaminated samples (25, 40 and 70 ppb) from highly contaminated samples (200, 300 and 500 ppb) without any overlapping of data. The maximum classification accuracy of 94.7% was obtained using PLS-DA with SNV pre-processed data. Across all the combinations of pre-processing and classification models, the best efficiency (98.2%) was exhibited by k-NN model with raw data. The developed PLS model depicted good prediction accuracy ( R CV 2  = 0.820, SE CV  = 79.425, RPD CV  = 2.382) during Venetian-blinds cross-validation. The results of pixel-wise classification (k-NN) and concentration distribution maps (PLS with raw spectra) were quite close to the result obtained by reference method (HPLC analysis) of aflatoxin-B1 detection., (© Association of Food Scientists & Technologists (India) 2020.)

The overuse of nitrogenous fertilizers leads to an increase in the nitrate content of green leafy vegetables. Consumption of food with excess nitrate is not advisable because it results in human ailment. In this study, spinach leaves were harvested from plants grown under nine varying (0 to 400 kg/ha) nitrogenous fertilizer doses. A total of 261 samples were used to predict the nitrate content in spinach leaves using Vis-NIR (350 to 2,500 nm). The nitrate content was measured destructively using the ion-selective conductive method. Partial least square (PLS) regression models were developed using whole spectra and featured wavelengths. Spectral data were pre-processed using different spectral pre-processing techniques such as Savitzky-Golay (SG) derivative, standard normal variate (SNV), multiplicative scatter correction (MSC), baseline correction, and detrending. The predictive accuracy of the PLS model had improved after pre-processing of spectral data with MSC (RPD CV = 1.767; SE CV = 545.745; bias CV = -3.107; slope CV = 0.698) and SNV (RPD CV = 1.768; SE CV = 545.337; bias CV = -3.201; slope CV = 0.698) technique, but this was not significant (P < 0.05) as compared with raw spectral data (RPD CV = 1.679; SE CV = 572.669; bias CV = -7.046; slope CV = 0.687). The effective wavelengths for measurement nitrate content in spinach leaves were identified as 558, 706, 780, 1,000, and 1,420 nm. The performance of PLS model developed with effective wavelengths also had good prediction accuracy (RPD CV = 1.482; SE CV = 648.672; bias CV = -3.805; slope CV = 0.565) but significantly lower than the performance of model developed with full spectral data. The overall results of this study suggest that Vis-NIR spectroscopy can be an important tool and has great potential for the rapid and nondestructive assessment of nitrate content in harvested spinach, with a view to ascertain the suitability of the harvest for food uses. PRACTICAL APPLICATION: Better production and brighter color of leafy vegetable drive the farming community to overuse nitrogenous fertilizer. This has resulted in higher nitrate content in vegetables. It has been widely reported that consumption of these vegetables has carcinogenic effects on human beings. The prediction of nitrate content in leafy vegetables by traditional methods is time-consuming (30 min, including sample preparation time), destructive, and tedious; moreover, it cannot be used for inline applications. This study reports spectroscopy-based rapid (<5 s) assessment technique for nitrate measurement. A multivariable PLS model was developed using wavelengths representing nitrate content. This model can be adopted by food industries for inline applications., (© 2020 Institute of Food Technologists®.)

The present study emphasizes on the antimicrobial susceptibility of different bacterial strains isolated from the external body surface of a commonly found zooplanktonic copepod (Heliodiaptomus viduus, Gurney, 1916) inhabiting both in fresh and brackish water bodies of Midnapore (West and East) Districts, West Bengal, India. Out of 62 bacterial isolated strains, 38 isolates were identified as Gram-positive while the remaining 24 isolates were found to be Gram-negative. Antimicrobial properties of all those bacterial strains were determined by Vitek 2 compact system using minimum inhibitory concentration (MIC) values. All isolated bacterial strains had exhibited differential susceptibilities against some selected antibiotics. Field Emission Scanning Electron Microscope (FE-SEM) analysis revealed the considerable association of bacteria on the cuticular body parts of the studied zooplankton. The outcomes of the present research are expected to enable health professionals in identifying two major problems -1) bacterial association with zooplankton which is so far mostly considered as a novel source of food for fish in aquatic ecosystems. 2) Selection of antibiotics as treatment measure because of the pathogenic effects of zooplankton associated bacteria on human being. This unattended arena of research is also supposed to evoke a new dimension not only because of bacteria-zooplankton interactions but also on undertaking of judicious strategies to find out proper ways and means to make the surface water suitable for the utilization by the common peoples (minimising bacterial contamination) in the context of human health and environmental safety., (Copyright © 2019. Published by Elsevier Inc.)

The process of bioconversion of solid organic wastes through vermicomposting justifies the environmental message for sustainability such as reduce, recycle and reuse of wastes. In the present study, wastes derived from two different types of paper mill sludge (primary and secondary), was used for their bioconversion through the vermicomposting process using an indigenous species of earthworm ( Perionyx excavatus). The maturity and stability stage of vermicompost production was assessed using FT-IR, GC-MS and TG analyses. During vermicomposting, different biochemical functional groups present in the wastes have shown differential chemical alteration and turnover as revealed by FT-IR spectroscopy. This study has also confirmed the trend of biodegradation of complex substances like lignin, cellulose, proteins etc. and thereby demonstrates the extent of mineralization. TG spectral analysis had revealed a mass loss of 80% and 71% in vermicompost produced from primary and secondary sludge respectively. GC-MS studies have also shown the presence of several humic acids like octadecanoic acid, heptadecanoic acid etc. in the decomposing substances demonstrating as an indicator of the maturity of products. This was further confirmed by the decrease of humification index which focuses the combined action of both earthworms and microbes in the degradation of organic wastes. The present study has highlighted the role of an indigenous earthworm in converting specific industrial wastes especially by recording the point of maturation using humic acids an indicator of the quality of decomposing of wastes following several instrumental applications.

This study analyzes the trophic structure of fish assemblages in a well-mixed tropical estuary, Zuari, located in southwest coast of India. A total of 224 fish species were collected between September 2013 and August 2016 from 324 sampling events from 9 sampling stations using surface and bottom set gillnets. The sampling events were designed to cover three major seasons such as the pre-monsoon, monsoon, and post-monsoon. For each sampling event, abiotic variables such as temperature, salinity, dissolved oxygen, and transparency of the water were recorded. The data obtained for abundance of fish species was subjected to cluster analysis and non-metric multidimensional scaling. The results defined six spatio-temporally varying groups (clusters) within Zuari estuary. Canonical correspondence analysis demonstrated that temperature, salinity, dissolved oxygen, and transparency were important in determining the fish assemblages in Zuari. The estuarine resident species were abundant in the inner zone (riverine side) of the estuary, where the salinity and temperature recorded lower dimensions compared to middle and outer zones (marine side). However, the inner shallow zone of the estuary was not a preferred habitat for the migrating marine species. Majority of the fish species preferred middle and outer zones and post-monsoon and pre-monsoon seasons for inhabiting the estuary. The present study furnishes a reference data for the spatio-temporal dynamics of fish assemblages in tropical monsoonal estuaries.

The Indian river shad, Gudusia chapra (Hamilton, 1822), is a commercially important freshwater clupeid that contribute to the livelihood of subsistence and marginal fisheries in inland open waters of India and its adjacent countries. The present study was carried out on the growth and mortality of Indian River shad, Gudusia chapra (Hamilton, 1822) based on the commercial length-frequency data collected monthly during October 2014 to September 2015 of a large reservoir located in the border of Jharkhand and West Bengal. The ELEFAN-I (Electronic Length Frequency Analysis) module in FiSAT (FAO-ICLARM Stock Assessment Tools) was used for estimation of growth parameters (L ∞ , K). The total mortality was estimated following Pauly (ICLARM Fishbyte 2(1):9-13, Pauly 1983) whereas exploitation ratio and maximum sustainable yield were calculated following Gulland (1979). The asymptotic length (L ∞ ) and growth coefficient (K) were estimated as 194 mm and 1.23 year -1 , respectively. The species grows to 141.56 and 182.91 mm at the end of the 1st and 2nd years of life. The total natural and fishing mortalities were 6.63, 2.45 and 4.18 year -1 respectively. The value of growth performance index was 2.69. The exploitation ratio (E) was estimated as 0.63. As the exploitation ratio (E) is slightly beyond the optimum, there is likelihood of some threat of overexploitation. Though recruitment was continuous, major pulse was observed during April-May. The present investigation is a first report from Indian reservoir. The maximum sustainable yield of this species was estimated as 485.6 t against the present annual yield of 612.3 t. Avoiding overfishing and wise use of available resources are of prime importance for sustainable fishery management of the reservoir.

The present work deals with assessment of baseline radionuclide concentration in marine organisms around selected coasts of Maharashtra, India. This baseline study highlights concentrations of natural and fallout radionuclides in finfish and shellfish species found in the surrounding seawater. Water and fish samples were collected, processed, and analyzed for 238 U, 226 Ra, 228 Ra, 232 Th, 40 K, and 137 Cs by high-resolution gamma spectrometry. 238 U, 226 Ra, 228 Ra, 232 Th, 40 K, and 137 Cs concentrations were found to be in the range of ≤1.5, ≤2.0, ≤0.4, ≤1.5, ≤8.0 to 258, and ≤0.2 Bq/kg, respectively. The seasonal study revealed the maximum concentration in premonsoon season. Activity concentrations were higher in pelagic fish than those in demersal fish. A higher concentration factor was reported in shellfish than in finfish., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Background and Objectives: Fullerene-based compounds are a novel class of molecules being developed for a variety of biomedical applications, with nearly 1000 publications in this area in the last 4 years alone. One such compound, the e,e,e-methanofullerene(60)-63-tris malonic acid (designated C 3 ), is a potent catalytic superoxide dismutase mimetic which has shown neuroprotective efficacy in a number of animal models of neurologic disease, including Parkinsonian Macaca fascicularis monkeys. The aim of this study was to characterize its toxicity and pharmacokinetics in mice and monkeys., Methods: To assess pharmacokinetics in mice, we synthesized and administered 14 C-C 3 to mice using various routes of delivery, including orally. To assess potential toxicity in primates, serial blood studies and electrocardiograms (ECGs) were obtained from monkeys treated with C 3 (3 or 7 mg/kg/day) for 2  months., Results and Conclusions: The plasma half-life of C 3  was 8.2 ± 0.2 h, and there was wide tissue distribution, including uptake into brain. The compound was cleared by both hepatic and renal excretion. C 3 was quite stable, with minimal metabolism of the compound even after 7 days of treatment. The LD 50 in mice was 80 mg/kg for a single intraperitoneal injection, and was > 30 mg/kg/day for sustained administration; therapeutic doses are 1-5 mg/kg/day. For primates, no evidence of renal, hepatic, electrolyte, or hematologic abnormalities were noted, and serial ECGs demonstrated no alteration in cardiac electrical activity. Thus, doses of C 3 that have therapeutic efficacy appear to be well tolerated after 2 years (mice) or 2 months (non-human primates) of treatment.

Mass production of vermicompost using suitable species of earthworms and selecting target organic waste materials has appeared to be a great development in the realm of biotechnological research for the sustainable eco-management. Although, for the bioconversion of organic wastes to vermicompost, suitable earthworm species play major roles, a hoard of bacterial assemblages by virtue of production of different enzymes facilitate the process of vermicomposting. The present study has documented the roles of vermicompost associated bacteria in combating, preventing, and controlling of cancer so as to open a new vista not only in the field of vermitechnology but also on biomedical research. Earthworms' associated bacterial metabolic products having their unique physicochemical excellence have gained importance due to their roles as a facilitator of apoptosis (programed cell death in a MCF-7 cell line). The antioxidant and anticancer activities of ethyl acetate extracts' of vermicompost associated bacterium Bacillus anthracis were undertaken by antioxidant assay which revealed maximum DPPH radical scavenging effect (75.79 ± 5.41%) of the extracts' at 9 00  μ g ml-1. Furthermore, the crude extracts obtained from the same bacteria were found to decrease the activity of SOD (superoxide dismutase) with the increase in doses. MTT assay showed potent cytotoxic activity against human breast adenocarcinoma cells (MCF-7) with the IC50 value of 46.64 ± 0.79  μ g ml-1. It was further confirmed through Hoechst 33258 staining of nuclear fragmentation assay and DNA fragmentation analysis. Western blotting test has confirmed a downregulation of Akt upon application of crude extracts. Increase of SOD activity along with decrease of Akt level reflects that the mode of action is entirely PI-3K dependent. This study tends to indicate that B . anthracis isolated from vermicompost could be potentially explored for the development of new therapeutic agents, especially against cancer.

Owing to increasing concern of global climate-change, temperature rise is of great interest which can be primarily evaluated from the seasonal variations in some organisms. Aquatic environment can be extremely stressful to its inhabitants because most of them are poikilothermous. In the present study, attempt was made to evaluate the biological effects of oxidative-stress and adaptive/antioxidant capacities during temperature variations (36-40 °C for 24hrs to 72hrs) in Bellamya bengalensis both in environmental and laboratory conditions by testing some biomarkers like HSP70, catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH) and glutathione reductase (GR). The biomarker potency of the molecules and the anti-oxidative metabolic-network was postulated and extrapolated to find its resemblance to the climate-change associated organismal variations. In a natural and eco-restored environment in the Eastern part of India, 10-20 fold increases in CAT, SOD and HSP70 protein expressions (Western blot results) were noticed in Bellamya paralleling to their increased enzymatic activities (gel zymogram studies) due to the seasonal (summer versus winter) temperature variation. It is evident from the consecutive three years' study that this variation resulted in the unfavorable physico-chemical changes of water quality parameters like dissolved oxygen, biochemical oxygen demand, alkalinity and consequently decreased the animal density in summer. And that was revived due to their higher reproduction-rate in post rainy/winter season when temperature normalizes resulting in a restoration of favorable environment. In laboratory condition, the reduced GR and increased GPx indicated the oxidative damage as evident by higher tissue MDA level following to higher mortality. Changes in SOD and CAT activities suggest activation of physiological mechanism to scavenge the ROS produced during heat stress. However, when mortality increased at different time points (36 °C - 72 h and 38 °C - 72 h), these enzyme activities also decreased as they failed to save the tissues from ROS. The results suggest that temperature variation does alter the active oxygen metabolism by modulating antioxidant enzyme activities, which can be used as biomarker to detect sub-lethal effects of climate change-associated pollution. The parity in environmental and laboratory experimental results may justify this laboratory experiment as model heat-stress experiment and indicate temperature as a universal stressor which alone or in combination with other water parameters initiates a consistent adapting behavior. The Bellamya bengalensis being the highest faunal representative in its habitat may serve as a good bioindicator species., (Copyright © 2018. Published by Elsevier Inc.)

Purpose: Main thrust of the present study is to determine the role of microbes in changing the proportion and turnover of nutrients such as carbon and nitrogen during vermicomposting of paper mill sludge through the assessment of β-glucosidase and Leucine arylamidase activities., Methods: The change in the ratio of Total Organic Carbon (TOC) and Total Kjeldahl Nitrogen (TKN) during sludge composting using paper mill sludge, cow dung, straw in the ratio of 5:4:1 have been determined alongside observing β-glucosidase and Leucine arylamidase activities in different phases (0th, 30th and 60th days) of vermicomposting. The present study also assessed the bacterial assemblages in order to predict their role as major producers of these enzymes as estimated by Vitek 2 system., Results: A declining trend of C/N ratio was observed which exhibited significant negative and positive correlations with the activity of β-glucosidase and Leucine arylamidase respectively. Twenty-four strains were isolated under two genus Bacillus spp. and Lysinibacillus spp. which revealed differential sensitivity towards major carbon and nitrogen turnover mediating enzymes., Conclusion: Change in the activities of carbon and nitrogen turnover enzymes due to microbial growth and proliferation are the mediator for the change in C/N ratio., Competing Interests: Authors have no conflict of interest.Not applicable for this study.

Metachromadora (Bradylaimoides) benepapillata, was first discovered from the coastal mangroves of Bangladesh by Timm (1961). This species was later synonymised with Pseudochromadora cazca, which was described from the Brazilian coast by Gerlach (1956). The present paper proposes to update the taxonomic status of the Timm's species as Pseudochromadora benepapillata comb. n. along with its distribution and comparative morphology. The major differences between P. cazca and P. benepapillata are found in the characteristics of the head capsule; length of cephalic sensilla; length and position of somatic setae; shape of amphideal fovea; shape of spicules and gubernaculum; number and morphology of copulatory and postcloacal thorns. The present report on the occurrence of this species along the eastern most coast of India extends its distribution from the eastern part of Bay of Bengal to its western part.

The quality parameters of sauerkraut fermented using Lactobacillus paracasei in terms of its lactic acid bacteria count, texture, colour and biochemical properties were studied. As a starter culture L. paracasei grown in tofu whey was used for sauerkraut fermentation. The experiments were planned using central composite rotatable design of response surface methodology for input variables - culture volume (ml), fermentation time (days) and salt concentration (g/100 g). The linear and interactive effect of variables on responses was understood by statistically significant (p < 0.01) second-order models. Amongst all the input variables culture volume was found to have an overwhelming effect over all the responses. There was a significant (p < 0.01) increase in the lactic acid bacteria count of finished product; it was less hard but there was a departure in colour from the traditional product. The optimized condition for sauerkraut fermentation in terms of culture volume (ml), fermentation time (day) and salt concentration (g/100 g) was 30 ml, 28 days and 1 g/100 g, respectively. It was also observed that phenolics content was better in starter culture sauerkraut over the one traditionally prepared.

Reactive oxygen species (ROS) are believed to play a major role in the proinflammatory, M1-polarized form of neuroinflammation. However, it has been difficult to assess the role of ROS and their role in neuroinflammation in animal models of disease because of the absence of probes capable of measuring their presence with the functional imaging technique positron emission tomography (PET). This study describes the synthesis and in vivo evaluation of [ 18 F]ROStrace, a radiotracer for imaging superoxide in vivo with PET, in an LPS model of neuroinflammation. [ 18 F]ROStrace was found to rapidly cross the blood-brain barrier (BBB) and was trapped in the brain of LPS-treated animals but not the control group. [ 18 F] ox-ROStrace, the oxidized form of [ 18 F]ROStrace, did not cross the BBB. These data suggest that [ 18 F]ROStrace is a suitable radiotracer for imaging superoxide levels in the central nervous system with PET.

Fullerene-based compounds are being developed for an extensive range of biomedical applications, and may provide a completely new class of biologically useful reagents. In support of our continuing investigation and characterization of one such compound, e,e,e -fullerene(60)-63-tris malonic acid ( 1 ) we optimized the conditions for obtaining mass spectra. Both positive and negative ion mass spectra are obtained using electrospray ionization (ESI). However, the spectra are dramatically different in the different ionization modes. We studied the effect of solvent media, acid content as well as the concentration of the compound (1) on mass fragmentation pattern both in positive and negative mode. The best mass spectra were obtained when 1 was sprayed from a solution containing a weak organic acid added to aqueous methanol (1:1) in positive mode. We also analyzed the ion current as function of capillary voltage for selected ion. Fragment ions formed by the direct loss of carboxyl groups from the doubly-charged dimer occur for the loss of one, two and six carboxyl groups. Of these, the loss of one carboxyl is the most abundant. The dominant mechanism for the formation of singly-charged fragment ions arises from splitting of the doubly-charged dimers into singly-charged monomers with subsequent carboxyl losses.

The effects of formulation of leavened bread by using varying levels (for 50 g base flour) of soy flour-barnyard millet blends (with 5.74, 6.25, 7, 7.75 and 8.26 g of soy flour), yeast (1.83, 2, 2.25, 2.5 and 2.67 g) and salt (0.63, 0.8, 1.05, 1.30 and 1.47 g) on textural, colour and specific volume were determined. A central composite rotatable design of response surface methodology was used to plan the experiments. The second order models obtained were observed to be statistically significant and capable of demonstrating the effects input variables on responses. All the textural properties were affected significantly by amount of soy flour and yeast in the dough. Soy flour had a significant effect on the colour of the bread making it more brown. Interaction of soy flour and yeast affected the specific volume to maximum extent. Two-tailed t test established that the efficacy of the models as no significant was observed between the predicted and the actual values.

A new genus, Cyathoshiva, of free-living marine nematodes from the subfamily Cyatholaiminae (family Cyatholaimidae, order Chromadorida), is described from the Indian coast. The new genus differs from other known Cyatholaiminae genera in having a unique combination of morphological characters: buccal cavity with well-developed dorsal tooth and two subventral teeth, proximally unpaired gubernaculum and non-cup shaped supplements. The type species Cyathoshiva amaleshi gen. n. sp. n. is characterized by having multispiral amphids with 4.5 turns, punctated cuticle with a weak lateral differentiation, a conical tail, three supplements possessing both tubular and setoid parts, and a gubernaculum with a serrated and dentate distal end.

Background: Membrane proteins regulate a diversity of physiological processes and are the most successful class of targets in drug discovery. However, the number of targets adequately explored in chemical space and the limited resources available for screening are significant problems shared by drug-discovery centers and small laboratories. Therefore, a low-cost and universally applicable screen for membrane protein trafficking was developed., Results: This high-throughput screen (HTS), termed IRFAP-HTS, utilizes the recently described MarsCy1-fluorogen activating protein and the near-infrared and membrane impermeant fluorogen SCi1. The cell surface expression of MarsCy1 epitope-tagged receptors can be visualized by simple addition of SCi1. User-friendly, rapid, and quantitative detection occurs on a standard infrared western-blotting scanner. The reliability and robustness of IRFAP-HTS was validated by confirming human vasopressin-2 receptor and dopamine receptor-2 trafficking in response to agonist or antagonist. The IRFAP-HTS screen was deployed against the leucine-rich G protein-coupled receptor-5 (Lgr5). Lgr5 is expressed in stem cells, modulates Wnt/ß-catenin signaling, and is therefore a promising drug target. However, small molecule modulators have yet to be reported. The constitutive internalization of Lgr5 appears to be one primary mode through which its function is regulated. Therefore, IRFAP-HTS was utilized to screen 11,258 FDA-approved and drug-like small molecules for those that antagonize Lgr5 internalization. Glucocorticoids were found to potently increase Lgr5 expression at the plasma membrane., Conclusion: The IRFAP-HTS platform provides a versatile solution for screening more targets with fewer resources. Using only a standard western-blotting scanner, we were able to screen 5,000 compounds per hour in a robust and quantitative assay. Multi-purposing standardly available laboratory equipment eliminates the need for idiosyncratic and more expensive high-content imaging systems. The modular and user-friendly IRFAP-HTS is a significant departure from current screening platforms. Small laboratories will have unprecedented access to a robust and reliable screening platform and will no longer be limited by the esoteric nature of assay development, data acquisition, and post-screening analysis. The discovery of glucocorticoids as modulators for Lgr5 trafficking confirms that IRFAP-HTS can accelerate drug-discovery and drug-repurposing for even the most obscure targets.

Optical imaging of whole, living animals has proven to be a powerful tool in multiple areas of preclinical research and has allowed noninvasive monitoring of immune responses, tumor and pathogen growth, and treatment responses in longitudinal studies. However, fluorescence-based studies in animals are challenging because tissue absorbs and autofluoresces strongly in the visible light spectrum. These optical properties drive development and use of fluorescent labels that absorb and emit at longer wavelengths. Here, we present a far-red absorbing fluoromodule-based reporter/probe system and show that this system can be used for imaging in living mice. The probe we developed is a fluorogenic dye called SC1 that is dark in solution but highly fluorescent when bound to its cognate reporter, Mars1. The reporter/probe complex, or fluoromodule, produced peak emission near 730 nm. Mars1 was able to bind a variety of structurally similar probes that differ in color and membrane permeability. We demonstrated that a tool kit of multiple probes can be used to label extracellular and intracellular reporter-tagged receptor pools with 2 colors. Imaging studies may benefit from this far-red excited reporter/probe system, which features tight coupling between probe fluorescence and reporter binding and offers the option of using an expandable family of fluorogenic probes with a single reporter gene.

The knowledge on the reproductive biology of fish Oreochromis niloticus (Linnaeus, 1758) in riverine condition is meagre in the Indian sub-continent which is necessary for the development of an appropriate fishery management protocols for the rivers. In this context, the gonadal maturation profile and fecundity of 0. niloticus was studied. Mature, spawning and spent females recorded in almost all the months of the year evidenced multiple and prolonged breeding behaviour. An overall sex ratio (M/F) of 1: 1.08 observed was statistically non-significant from expected 1:1 ratio (chi2=6.994219, p=0.8642) indicating that males and females were statistically equal in number. The absolute fecundity ranged from 1192 to 4760 with mean of 2590 eggs from ovary weighing between 1.91 g to 28.89 g. Monthly changes in gonado-somatic index (GSI) revealed that fish bred throughout the year except in July and August, but at peak between October-November and March-June. Size at first maturity of male was estimated as 229.6 mm with confidence limit of 235.9 and 223.6 mm and for female as 238.1 mm with confidence limit of 244.7 and 231.7 mm, respectively.

Designed experiments were conducted to study the rheological properties of baking dough prepared from different refined wheat flour (RWF) - barnyard millet blends with varying amount of water (WA), salt and sugar. Dough was subjected to thermo-mechanical stress in Mixolab, in which rheological properties were recorded in terms of five different torques. Second order polynomial models were developed using response surface methodology (RSM) to understand the effect of input variables (WA, barnyard millet, salt and sugar; all expressed as per cent of base flour) on torques recorded by Mixolab. Optimum values of input variables were obtained with constraints based on torque values which represented the qualities of acceptable bread dough. The models predicted that a dough with 57, 26, 1.8 and 3.3% of water, barnyard millet, salt and sugar, respectively, can be used for bread baking purposes.

Gobiids constitute a major proportion of fish population in both tropical and temperate freshwater as well as marine ecosystem. Due to their small size, cryptic ecology and ambiguous morphological characters, gobiids diversity was not documented completely. In this study, DNA barcodes were generated for 11 species of gobiids, collected from the Ashtamudi Lake, India. The mitochondrial COI gene was amplified using universal primers and the resulted 650 bp amplicon was sequenced. The COI barcodes clearly distinguished all the species with high inter-specific genetic distance values than intra-specific values based on K2P (Kimura 2 Parameter) model. The average genetic distance (K2P model) within species, genus and family was 1.2%, 22.2% and 25.3%, respectively. In addition to barcode-based species identification system, Nucleotide Diagnostic (ND) characters specific for species were identified. The Neighbor-Joining tree revealed distinct clusters shared by the species of same genera.

Whey is a nutritious by product of some traditional Indian processed milk products and it needs to be utilized in an effective way in order to reduce environmental hazards associated with its untreated disposal. Low calorie watermelon beverage appears to be a simple, attractive and economic method of whey disposal. The experiment was designed by Central Composite Rotatable Design of Responce Surface Methodology. Three independent variables whey, Innova ® fiber and sucralose were chosen at five levels within the respective ranges of 40-60 %, 2.0-5.0 % and 0.01-0.03 %. The effect of the variables on flavour, mouthfeel, after-taste, viscosity, total soluble solids (all to be maximized) and sedimentation (to be minimized) was observed. These three were the independent variables whose effect on flavour, mouthfeel, after-taste, viscosity, total soluble solids (all to be maximized) and sedimentation (to be minimized) were evaluated. Quadratic model fitted well to all dependent variables. The R(2) values for flavour, mouthfeel, aftertaste, viscosity, sedimentation and TSS were 95.57, 98.71, 95.50, 97.87, 99.26 and 98.17 %, respectively. Response surface methodology was used to optimize the level of processing parameters. Maximum scores for flavour (7.46), mouthfeel (7.49), after-taste (7.72), viscosity (13.55 cp) and total soluble solid (15.34°Brix) and minimum score for sedimentation (1.55 ml/10 ml) were obtained when the formulation contained 51.46 % whey, 3.84 % Innova® fiber and 0.021 % sucralose.

Identification of elasmobranchs by conventional taxonomy is difficult due to similarities in morphological characters. Species-specific molecular markers are good choice for identifying species irrespective of it's life stage. Recently, mitochondrial cytochrome c oxidase subunit I (COI) gene got global recognition as a barcode gene to discriminate all animals up-to species level. In this study, mitochondrial COI partial gene was used to develop DNA barcodes for 18 species of elasmobranchs (10 species of sharks and 8 species of rays). The COI barcodes clearly distinguished all the species with high interspecific distance values than intraspecific values. The average interspecific and intraspecific distance values are 8.6% and 0.3% for sharks, respectively and 12.4% and 0.63% for rays, respectively using K2P method. The Neighbor-Joining tree showed distinct clusters shared by the species of same genera. The COI barcodes were also used to estimate allopatric divergences for selected species across broad geographical locations and found that Sphyrna lewini, Aetobatus narinari and Neotrygon kuhlii have cryptic diversity.

Expression of the stress biomarkers 70-kDa heat shock proteins (Hsp70) and manganese superoxide dismutase (MnSOD) was measured as the molecular basis of adaptive response against increased experimental temperatures (32-40 °C for a span of 24-72 h) on the fresh water molluscan species, Bellamya bengalensis (Lamark 1882). The experimental snail specimens were collected during summer and winter seasons from two contrasting wetlands: an ecorestored (free from human interference) site (SI) and other experiencing anthropogenic stresses (SII). The mortality rate of the B. bengalensis and the immunoblotting of MnSOD and Hsp70 of their digestive glands were performed at regular intervals during the period of heat stress. The SI provided a lower stress environment based on physicochemical parameters such as pH, dissolved oxygen (DO), biological oxygen demand (BOD), chemical oxygen demand (COD), and alkalinity for the survival of test species, although both sites experienced mortality due to thermal stresses. The parity in protein expressions displayed a uniform mode of adaptive impact to temperature elevations in both field and laboratory exposure. The Hsp70 expression was minimal at lower thermal stress, but increased with a rise in temperature. It is very likely that higher Hsp70 levels are not directly related to survival or adaptation. In contrast, MnSOD levels appeared to be an indicator of adaptive responses vis-a-vis survival of the animals. So, the expression levels of a universal free radical scavenger like MnSOD are recognized as a potential biomarker in a bioindicator species like Bellamya.

The fluorescence of the SKC-513 ((E)-N-(9-(4-(1,4,7,10,13-pentaoxa-16-azacyclooctadecan-16-yl)phenyl)-6-(butyl(3-sulfopropyl)amino)-3H-xanthen-3-ylidene)-N-(3-sulfopropyl)butan-1-aminium) dye is shown experimentally to have high sensitivity to binding of the K(+) ion. Computations are used to explore the potential origins of this sensitivity and to make some suggestions regarding structural improvements. In the absence of K(+), excitation is to two nearly degenerate states, a neutral (N) excited state with a high oscillator strength, and a charge-transfer (CT) state with a lower oscillator strength. Binding of K(+) destabilizes the CT state, raising its energy far above the N state. The increase in fluorescence quantum yield upon binding of K(+) is attributed to the increased energy of the CT state suppressing a nonradiative pathway mediated by the CT state. The near degeneracy of the N and CT excited states can be understood by considering SKC-513 as a reduced symmetry version of a parent molecule with 3-fold symmetry. Computations show that acceptor-donor substituents can be used to alter the relative energies of the N and CT state, whereas a methylene spacer between the heterocycle and phenylene groups can be used to increase the coupling between these states. These modifications provide synthetic handles with which to optimize the dye for K(+) detection.

Watermelon juice was exposed to the enzyme masazyme at varying enzyme concentrations (0.01-0.1 % w/w) and different time (20-120 min) and temperature (30-50 °C) combinations. The effects of the treatments on selected responses (juice recovery, total dissolved solids (TDS), viscosity, turbidity, cloud stability and L value) were determined employing a second order Box Behnken Design in combination with Response Surface Methodology. Enzymatic treatment effectively degraded polysaccharides, resulting in reduced viscosity, turbidity and absorbance value and increased juice recovery, total dissolved solids and lightness. R(2) value for all models for the dependent variables were greater than 90 %. The maximum juice recovery (86.27 %), TDS (8.7°Brix) and L value (17.57) while minimum viscosity (0.0020 Pa.s.), turbidity (39.37 NTU) and cloud stability (0.033 abs) were obtained when enzyme treatment was set up with 0.09 % w/w enzyme concentration at 46.90 °C and 117.45 min.

Umbilical cord blood collected at birth is a rich source of stem cells that can be used to treat diseases of the blood and immune system. Cord blood stem cells which have infinite medical potential are currently used in the treatment of several life-threatening diseases, and play an important role in the treatment of blood and immune system related genetic diseases, cancers, and blood disorders. Transplant recipients are less likely to reject a cord blood stem cell and do not require a perfect match as bone marrow transplants do. Transplantation of umbilical cord blood stem cells represents a major advantage in providing a new source of stem cells to patients in need. Private storage of cord blood stem cells clearly has value, in that it provides future patients and families with potential therapeutic options for transplant. Many recent studies showed that human umbilical cord blood stem cells have the potential to generate cells with neuronal characteristics. Therefore, the umbilical cord blood stem cells can be viewed as the stem cells source of choice for clinical and non-clinical research applications.

This study aimed to determine the cellular alterations in the gill of Labeo rohita exposed to lethal temperature maxima (LTM ax ) and lethal temperature minima (LTM in ) by means of transmission electron microscopy (TEM). Acclimation of advanced fingerlings of L. rohita was carried out at 26°C for 30 days. Acclimated fish were subjected to a constant rate of increase or decrease in temperature (0.3°C/min) until the LTM ax and LTM in values were reached. Dissected gills were processed for TEM, both at the end of acclimation period at ambient temperature (26°C) and at lethal temperatures. Results indicated that at ambient temperature, the gill tissues appeared normal. However, significant changes were observed at lethal temperatures. The gill tissues at lethal temperature maxima showed severely damaged lamellae, with more vacuolated space. At lethal temperature minima, gill tissues showed increased density of mitochondria. Our prima-facie report indicated that L. rohita exposed to lethal temperatures exhibited marked ultrastructural changes in the gills., (© 2013 Blackwell Verlag GmbH.)

The elasmobranchs (sharks, rays and skates) being the extant survivors of one of the earliest offshoots of the vertebrate evolutionary tree are good model organisms to study the primitive vertebrate conditions. They play a significant role in maintaining the ecological balance and have high economic value. Due to over-exploitation and illegal fishing worldwide, the elasmobranch stocks are being decimated at an alarming rate. Appropriate management measures are necessary for restoring depleted elasmobranch stocks. One approach for restoring stocks is implementation of conservation measures and these measures can be formulated effectively by knowing the evolutionary relationship among the elasmobranchs. In this study, a total of 30 species were chosen for molecular phylogeny studies using mitochondrial cytochrome c oxidase subunit I, 12S ribosomal RNA gene and nuclear Internal Transcribed Spacer 2. Among different genes, the combined dataset of COI and 12S rRNA resulted in a well resolved tree topology with significant bootstrap/posterior probabilities values. The results supported the reciprocal monophyly of sharks and batoids. Within Galeomorphii, Heterodontiformes (bullhead sharks) formed as a sister group to Lamniformes (mackerel sharks): Orectolobiformes (carpet sharks) and to Carcharhiniformes (ground sharks). Within batoids, the Myliobatiformes formed a monophyly group while Pristiformes (sawfishes) and Rhinobatiformes (guitar fishes) formed a sister group to all other batoids.

This observational study was done among the different types of diabetes, gestational diabetes mellitus (GDM). In the present study we looked for the microscopic impacts of GDM on the umbilical cord. This study was carried out in the department of Anatomy, Chittagong Medical College from May 2006 to April 2007. Total fifty umbilical cords with placenta were collected from selected subjects within 36 to 40th weeks of gestation and divided them into two groups. Twenty five control group (specimen collected from normal mothers) and 25 GDM group (specimen collected from mothers developing diabetes during pregnancy state). Tissues from a particular area of the umbilical cord were processed for histological study by routine procedure. All the slides were examined under light microscope. In GDM group light microscopy showed rupture and erosion of the endothelium and complete rupture of the umbilical vessels resulting in extravasation of blood within Wharton's jelly. The umbilical vein was grossly dilated and thin walled. Smooth muscles of the umbilical vessel showed disruption and degeneration of its fibers. Wharton's jelly showed alteration in the pattern of distribution and degeneration of its fibers with honeycomb like empty spaces among them. It is suggested that GDM might have some harmful effects on the umbilical vessels and connective tissue component of the Wharton's jelly.