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Earth observation satellite-04 or EOS-04 (Radar imaging satellite-1A or RISAT-1A) provides the opportunity for characterization of surface features using C-band fully-polarimetric, hybrid-polarimetric and dual-polarimetric radar data from same platform in high to moderate resolution (2–50 m depending on mode) and varied incidence angle (12°–55°). These unique EOS-04 radar datasets can be processed using Microwave Data Analysis Software (MIDAS) tool, to derive radar polarimetric parameters. MIDAS is capable of carrying out synthetic aperture radar (SAR) data processing comprising radiometric and majority of the polarimetric processing of EOS-04 datasets. In addition, MIDAS has the functionality to orthorectify and geocode EOS-04 polarimetric products, which is missing in other softwares. Further, MIDAS contains module for bulk-processing of timeseries EOS-04 products. We showcase results obtained from EOS-04 datasets and describe the scattering behaviour of various targets. Further, we also demonstrate the temporal variation in the backscatter values from different target features obtained from EOS-04 time-series stack, which has strong applicability in land use/land cover and agricultural applications. Thus, the radiometric and polarimetric products can be highly beneficial for characterization of physical properties of the scatterers based on their radar scattering behaviour and further classification of the surface features. [ABSTRACT FROM AUTHOR]

We assessed the immunological status of stage III and IV head and neck squamous cell carcinoma (HNSCC) patients and age-matched healthy individuals. In HNSCC patients, the total leukocyte count was lower and the proliferating ability of PBMCs against phytohemagglutinin (PHA) was significantly downregulated. These cells showed lower expression of the early activation marker CD69. Within this PBMC population, the proportion of CD4+, CD8+ T cells, CD3- CD56+, CD16+ NK cells and CD3+ CD56+ NK-T cells was seriously downregulated. However, the proportion of CD4+ CD25+ Foxp3+ regulatory T cells having suppressor function was upregulated. Other immune cells, like CD14+ monocytes/macrophages and CD20+ B cells, were also fewer in number, although this difference was not statistically significant. Assessment of the cytokine secretory status of PBMCs revealed suppressed levels of Th1 cytokines (IFN-gamma, IL-12 and TNF-alpha) and elevated secretion of Th2 cytokines (IL-4 and IL-10) for HNSCC PBMCs whereas just the opposite was seen for PBMCs from healthy individuals. Dysregulation in the profile of immunocompetent cells and cytokine secretion was reflected in the suppressed cytotoxic function of HNSCC PBMCs, as tested on KB (oral cancer), MCF7 (breast cancer), COLO205 (colon cancer), Jurkat (T cell leukemia), K562 (erythroleukemia) and U937 (monocytic lymphoma) cell lines. The observed decreased cytotoxicity of HNSCC PBMCs may be due to the downregulated expression of cytotoxic molecules (perforin, granzymeB and FasL) in HNSCC PBMCs. Assessment of the extent of immune dysfunction might help design immunotherapeutic protocols by incorporating any agent having immunomodulatory function.