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3 results on '"Chai, Chuan Jian"'

1. Population Structure of the Blue Swimmer Crab Portunus pelagicus in Coastal Areas of Malaysia Inferred from Microsatellites

Author

Chai, Chuan Jian, Bin Esa, Yuzine, Ismail, Syukri, and Kamarudin, Mohd. Salleh

Subjects
Research Article
Abstract

Chuan Jian Chai, Yuzine Bin Esa, Muhammad Fadhil Syukri Ismail, and Mohd. Salleh Kamarudin (2017) Portunus pelagicus, distributed throughout the Indo-West Paci c region, is one of the large and edible species of blue swimmer crabs. Increasing demand for the frozen and canned crabmeat industry worldwide has now relied mainly on P. pelagicus which in turn generates splendid income for the fisherman communities. In the present study, the population genetic structure of P. pelagicus was examined using six pairs of microsatellite loci. A total of 87 crab samples were collected from five different coastal areas of Malaysia. Genomic DNA was extracted from each sample for polymerase chain reaction (PCR) amplification and fragment analysis. Four out of six microsatellite primers revealed polymorphic loci in P. pelagicus sampled. The number of alleles per locus in P. pelagicus ranged from 14 to 34. Microsatellites analyses indicated low levels of genetic differentiation among the P. pelagicus populations. The average observed heterozygosity (HO = 0.48) obtained was lower than the standard heterozygosity found in most marine populations (H(O) = 0.79). The high F(IS) values (mean F(IS) = 0.4756) and low F(ST) values (mean F(ST) = 0.0413) also suggested the existence of inbreeding among different populations of P. pelagicus. In conclusion, this study was able to shed light on the population structure of P. pelagicus in coastal areas of Malaysia.

Published
2017

2. Phylogenetic relationships and population structure of Portunus pelagicus in the coastal waters of Malaysia inferred from mitochondrial DNA and microsatellites

Author

Chai, Chuan Jian and Chai, Chuan Jian

Abstract

The abundance of blue swimmer crabs, Portunus pelagicus throughout the Indo-West Pacific region makes it a valuable target species for fishery industry. The increasing demands of P. pelagicus have led to a growing interest on the broodstock selection of this particular crab species. This study examined the phylogenetic relationships, demographic history and population structure of P. pelagicus through sequence analyses of mitochondrial cytochrome c oxidase I (COI) gene (600 bp) as well as fragment analyses of four pairs of microsatellite loci. A total of 109 crab samples were collected from five different populations throughout the coastal areas of Malaysia. These include Perak (Pantai Remis and Kuala Sepetang), Johor (Pendas), Negeri Sembilan (Port Dickson), Terengganu (Besut) and Sarawak (Bako). In total, 15 haplotypes were obtained with 13 unique haplotypes and two shared haplotypes. The phylogenetic analyses via the Neighbour Joining (NJ), Maximum Parsimony (MP) and Maximum Likelihood (ML) methods supported the monophyletic status between P. pelagicus and P. sanguinolentus with high bootstrap value (100%). All three phylogenetic trees revealed similar tree topologies with differences in the bootstrap values. The clustering of all P. pelagicus samples into a single clade suggested that this species possibly belonged to a single species. The genetic identity of P. pelagicus was further elucidated with low genetic distances among the haplotypes (0.2-1.7%) via mitochondrial analyses. Limited variations were found among the populations of P. pelagicus with high haplotype diversity and low nucleotide diversity detected within each population. No hybrid individual was discovered based on the genetic analyses of P. pelagicus from sympatric sampling locations. Low FST values obtained among these populations also clarified that these crabs were compatible for breeding programs. One interesting finding of this study was the sharing of haplotypes between samples from Sarawa

Published
2017

3. Population Structure of the Blue Swimmer Crab Portunus pelagicus in Coastal Areas of Malaysia Inferred from Microsatellites.

Author

Chai CJ, Bin Esa Y, Ismail S, and Kamarudin MS

Abstract

Chuan Jian Chai, Yuzine Bin Esa, Muhammad Fadhil Syukri Ismail, and Mohd. Salleh Kamarudin (2017) Portunus pelagicus , distributed throughout the Indo-West Paci c region, is one of the large and edible species of blue swimmer crabs. Increasing demand for the frozen and canned crabmeat industry worldwide has now relied mainly on P. pelagicus which in turn generates splendid income for the fisherman communities. In the present study, the population genetic structure of P. pelagicus was examined using six pairs of microsatellite loci. A total of 87 crab samples were collected from five different coastal areas of Malaysia. Genomic DNA was extracted from each sample for polymerase chain reaction (PCR) amplification and fragment analysis. Four out of six microsatellite primers revealed polymorphic loci in P. pelagicus sampled. The number of alleles per locus in P. pelagicus ranged from 14 to 34. Microsatellites analyses indicated low levels of genetic differentiation among the P. pelagicus populations. The average observed heterozygosity ( H O = 0.48) obtained was lower than the standard heterozygosity found in most marine populations ( H O = 0.79). The high F IS values (mean F IS = 0.4756) and low F ST values (mean F ST = 0.0413) also suggested the existence of inbreeding among different populations of P. pelagicus . In conclusion, this study was able to shed light on the population structure of P. pelagicus in coastal areas of Malaysia.

Published
2017
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