1. Genomics and proteomics approaches to the study of cancer-stroma interactions.
- Author
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Rodrigues-Lisoni FC, Peitl P Jr, Vidotto A, Polachini GM, Maniglia JV, Carmona-Raphe J, Cunha BR, Henrique T, Souza CF, Teixeira RA, Fukuyama EE, Michaluart P Jr, de Carvalho MB, Oliani SM, Tajara EH, Cury PM, de Carvalho MB, Dias-Neto E, Figueiredo DL, Fukuyama EE, Góis-Filho JF, Leopoldino AM, Mamede RC, Michaluart-Junior P, Moyses RA, Nóbrega FG, Nóbrega MP, Nunes FD, Ojopi EF, Serafini LN, Severino P, Silva AM, Silva WA Jr, Silveira NJ, Souza SC, Tajara EH, Wünsch-Filho V, Amar A, Bandeira CM, Braconi MA, Brandão LG, Brandão RM, Canto AL, Cerione M, Cicco R, Chagas MJ, Chedid H, Costa A, Cunha BR, Curioni OA, Fortes CS, Franzi SA, Frizzera AP, Gazito D, Guimarães PE, Kaneto CM, López RV, Macarenco R, Magalhães MR, Meneses C, Mercante AM, Pinheiro DG, Polachini GM, Rapoport A, Rodini CO, Rodrigues-Lisoni FC, Rodrigues RV, Rossi L, Santos AR, Santos M, Settani F, Silva FA, Silva IT, Souza TB, Stabenow E, Takamori JT, Valentim PJ, Vidotto A, Xavier FC, Yamagushi F, Cominato ML, Correa PM, Mendes GS, Paiva R, Ramos O, Silva C, Silva MJ, and Tarlá MV
- Subjects
- Annexin A5 metabolism, Apoptosis, Cell Proliferation, Down-Regulation, Electrophoresis, Gel, Two-Dimensional, Fibroblasts metabolism, Genomics, Hep G2 Cells, Humans, Keratins metabolism, Mouth Neoplasms genetics, Nucleic Acid Hybridization, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Stromal Cells metabolism, Vimentin metabolism, Gene Expression Regulation, Neoplastic, Mouth Neoplasms metabolism, Proteome metabolism
- Abstract
Background: The development and progression of cancer depend on its genetic characteristics as well as on the interactions with its microenvironment. Understanding these interactions may contribute to diagnostic and prognostic evaluations and to the development of new cancer therapies. Aiming to investigate potential mechanisms by which the tumor microenvironment might contribute to a cancer phenotype, we evaluated soluble paracrine factors produced by stromal and neoplastic cells which may influence proliferation and gene and protein expression., Methods: The study was carried out on the epithelial cancer cell line (Hep-2) and fibroblasts isolated from a primary oral cancer. We combined a conditioned-medium technique with subtraction hybridization approach, quantitative PCR and proteomics, in order to evaluate gene and protein expression influenced by soluble paracrine factors produced by stromal and neoplastic cells., Results: We observed that conditioned medium from fibroblast cultures (FCM) inhibited proliferation and induced apoptosis in Hep-2 cells. In neoplastic cells, 41 genes and 5 proteins exhibited changes in expression levels in response to FCM and, in fibroblasts, 17 genes and 2 proteins showed down-regulation in response to conditioned medium from Hep-2 cells (HCM). Nine genes were selected and the expression results of 6 down-regulated genes (ARID4A, CALR, GNB2L1, RNF10, SQSTM1, USP9X) were validated by real time PCR., Conclusions: A significant and common denominator in the results was the potential induction of signaling changes associated with immune or inflammatory response in the absence of a specific protein.
- Published
- 2010
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