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31 results on '"Cerda-Nicolas, Miguel"'

23. Correlation between EGFR Amplification and the Expression of MicroRNA-200c in Primary Glioblastoma Multiforme.

Author

Serna, Eva, Lopez-Gines, Concha, Monleon, Daniel, Muñoz-Hidalgo, Lisandra, Callaghan, Robert C., Gil-Benso, Rosario, Martinetto, Horacio, Gregori-Romero, Aurelia, Gonzalez-Darder, Jose, and Cerda-Nicolas, Miguel

Subjects
GLIOBLASTOMA multiforme, EPIDERMAL growth factor receptors, FLUORESCENCE in situ hybridization, MICRORNA, GENE expression, BRAIN banks, GENE amplification
Abstract

Extensive infiltration of the surrounding healthy brain tissue is a critical feature in glioblastoma. Several miRNAs have been related to gliomagenesis, some of them related with the EGFR pathway. We have evaluated whole-genome miRNA expression profiling associated with different EGFR amplification patterns, studied by fluorescence in situ hybridization in tissue microarrays, of 30 cases of primary glioblastoma multiforme, whose clinicopathological and immunohistochemical features have also been analyzed. MicroRNA-200c showed a very significant difference between tumors having or not EGFR amplification. This microRNA plays an important role in epithelial-mesenchymal transition, but its implication in the behavior of glioblastoma is largely unknown. With respect to EGFR status our cases were categorized into three groups: high level EGFR amplification, low level EGFR amplification, and no EGFR amplification. Our results showed that microRNA-200c and E-cadherin expression are down-regulated, while ZEB1 is up-regulated, when tumors showed a high level of EGFR amplification. Conversely, ZEB1 mRNA expression levels were significantly lower in the group of tumors without EGFR amplification. Tumors with a low level of EGFR amplification showed ZEB1 expression levels comparable to those detected in the group with a high level of amplification. In this study we provide what is to our knowledge the first report of association between microRNA-200c and EGFR amplification in glioblastomas. [ABSTRACT FROM AUTHOR]

Published
2014
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31. Recombinations of chromosomal bands 10q24, 12q14-q15, and 14q24 in two cases of pulmonary chondroid hamartoma studied by fluorescence in situ hybridization.

Author

Gregori-Romero MA, Lopez-Gines C, Cerda-Nicolas M, Collado M, and Llombart-Bosch A

Subjects
Adult, Humans, In Situ Hybridization, Fluorescence, Karyotyping, Male, Middle Aged, Chromosomes, Human, Pair 10 genetics, Chromosomes, Human, Pair 12 genetics, Chromosomes, Human, Pair 14 genetics, Hamartoma genetics, Lung Diseases genetics, Translocation, Genetic genetics
Abstract

Pulmonary chondroid hamartomas (PCH) are benign mesenchymal tumors consisting of at least two cytogenetic subgroups. These subgroups are defined by chromosomal alterations at either 12q14-q15 or 6p21. Cytogenetic analysis of short-term cultures from two PCHs revealed two different rearrangements with 12q14 -q15. One of these had a unique translocation t(12;14)(q14-15;q24) with presence of two normal chromosomes 12 and a der(14), but missing the der(12). The other showed a complex rearrangement between chromosomes 10 and 12 with two different derivatives. Our data have been confirmed with fluorescence in situ hybridization analysis. These cases represent variant forms of the standard translocations.

Published
2003
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