Search

Your search keyword '"Cepleanu A"' showing total 82 results
Start Over Author "Cepleanu A"
82 results on '"Cepleanu A"'

1. Easy method for six-fragment Golden Gate Assembly of modular vectors

Author

Ionut Adrian Cepleanu-Pascu, Miruna Stan, Sebastian Cocioba, and Ileana Stoica

Subjects
cloning, Golden Gate, Golden Gate standard, mammalian expression vectors, S/MARs, Biology (General), QH301-705.5
Abstract

Efficient cloning techniques are a requirement for synthetic biology. This study provides a simplified cloning method based on Golden Gate Assembly that can be used for rapid vector construction. The building of multiple expression vectors with customizable modules is achieved in a timely manner with minimal hands-on time by removing unnecessary steps in the workflow. The authors constructed a total of 21 mammalian episomal expression vectors and conducted a fluorescence expression comparison for different regulatory region combinations post-transfection in HEK293T and HEPG2 cells. Screening revealed that using the EF-1α promoter in combination with the bovine growth hormone polyadenylation sequence seemed to perform best in the types of cells tested compared with other variants.

Published
2023
Full Text
View/download PDF

6. Novel Acetogenins from the Leaves ofAnnona purpurea

Author

Pablo N. Solis, Matthias Hamburger, Kazuhiro Ohtani, Mahabir P. Gupta, Kurt Hostettmann, and Felicitas Cepleanu

Subjects
biology, Chemistry, Silica gel, fungi, Organic Chemistry, Brine shrimp, Bacillus subtilis, Aedes aegypti, Fractionation, biology.organism_classification, Biochemistry, Catalysis, Inorganic Chemistry, Annona purpurea, chemistry.chemical_compound, Column chromatography, Annonaceae, Drug Discovery, Organic chemistry, Physical and Theoretical Chemistry
Abstract

The CH2Cl2 extract of the leaves Annona purpurea L. (Annonaceae) showed strong brine shrimp toxicity and pronounced activity against larvae of the yellow-fever mosquito Aedes aegypti. Activity-directed fractionation of the extract by a combination of column chromatography on silica gel, filtration, and high-pressure liquid chromatography led to the isolation of acetogenins 1–6 as the main active principles. The structures of two novel acetogenins named purpureacin 1(5) and purpureacin 2(6) were elucidated by spectroscopic analysis (UV, EI- and DCI-MS, EI-MS of Me3Si derivatives,1H- and 13C-NMR). The configuration of 5 and 6 was not established due to the limited sample amount. Compounds 3–6 also showed antifungal activity against Candida albicans, and 5 was slightly active against Bacillus subtilis.

Published
1993
Full Text
View/download PDF

7. [Multiple organ procurement: Lausanne experience]

Author

A, Cepleanu, P F, Cuénoud, F, Mosimann, R, Chioléro, J P, Gardaz, V, Aubert, and A, Genton

Subjects
Tissue and Organ Procurement, Cadaver, Heart Transplantation, Humans, Kidney Transplantation, Switzerland, Liver Transplantation
Published
1992

8. Dynamic connectivity management with an intelligent route service control point

Author

Van der Merwe, J., primary, Cepleanu, A., additional, D'Souza, K., additional, Freeman, B., additional, Greenberg, A., additional, Knight, D., additional, McMillan, R., additional, Moloney, D., additional, Mulligan, J., additional, Nguyen, H., additional, Nguyen, M., additional, Ramarajan, A., additional, Saad, S., additional, Satterlee, M., additional, Spencer, T., additional, Toll, D., additional, and Zelingher, S., additional

Published
2006
Full Text
View/download PDF

9. Reluctance in organ donation: A plea to re-target the information campaigns

Author

A. Cepleanu and F. Mosimann

Subjects
Male, Transplantation, education.field_of_study, medicine.medical_specialty, business.industry, media_common.quotation_subject, Population, Information policy, Social class, Tissue Donors, Surgery, Plea, Denial, Family medicine, Malpractice, medicine, Humans, Female, Organ donation, education, business, Switzerland, Health policy, media_common
Abstract

DURING the decade 1986 to 1995, the number of organ donors in Switzerland first remained relatively constant but started later to decrease significantly (Table 1). 1 The reasons for this disturbing tendency are puzzling because all transplant centers practice a very open information policy and their graft and patients survival rates meet the expected standards. In addition, patients' associations and Swiss Transplants have launched information campaigns, have organized national transplant days, and have kept positive relationships with the media. Finally, the legal and no case of malpractice has been reported. The aim of the present study is, therefore, to determine which social classes of the Swiss society, if any, are reluctant to accept the concept of organ donation.

Published
1997
Full Text
View/download PDF

14. Screening of Tropical Medicinal Plants for Molluscicidal, Larvicidal, Fungicidal and Cytotoxic Activities and Brine Shrimp Toxicity

Author

Cepleanu, F., Hamburger, M. O., Sordat, B., Msonthi, J. D., Gupta, M. P., Saadou, M., and Hostettmann, K.

Abstract

Crude Extracts Of 36 Medicinal Plants Collected In Africa and South America Were Tested For Their Molluscicidal (Biomphalaria Glabrata), Larvicidal (Aedes Aegypti), Fungicidal (Cladosporium Cu-Cumerinum) and Cytotoxic (Against 2 Human Colon Carcinoma Cell Lines) Activities, As Well As For Brine Shrimp Toxicity (Artemia Salina). Members Of The Annonaceae, Guttiferae and Leguminosae Are Shown To Contain Compounds Highly Active In These 5 Bioassays. Three Of The Species Tested Stand Out For Their Marked Activity In Only One Bioassay: Rogeria Adenophylla and Sesamum Indicum (Pe-Daliaceae) Demonstrate Potent Larvicidal Activity and Adenium Obesum (Apocynaceae) Exhibits Very Strong Cytotoxicity.

Published
1994
Full Text
View/download PDF

17. Response in measles antibodies of the guinea-pig treated with interferon inductors

Author

Y, Sorodoc, G, Danielescu, M, Cepleanu, C, Cernescu, and N, Cajal

Subjects
Male, Time Factors, Guinea Pigs, Newcastle disease virus, Hemagglutinins, Viral, Heart, Hemagglutination Inhibition Tests, Antibodies, Viral, Injections, Poly I-C, Measles virus, Spectrophotometry, Antibody Formation, Animals, Female, Interferons, Sindbis Virus, Injections, Intraperitoneal
Published
1973

20. [Evolution of several experimental virus diseases (influenza, poliomyelitis, Coxsackie virus disease, rabies, herpes) under the influence of radiophosphorus (P-32) and radioiodine (I-131)]

Author

N, CAJAL, O, BURDUCEA, S, MATEESCU, G, MARINESCU, M, CEPLEANU, and Y, COPELOVICI

Subjects
Iodine Radioisotopes, Rabies Vaccines, Rabies, Virus Diseases, Influenza, Human, Coxsackievirus Infections, Humans, Phosphorus, Dietary, Phosphorus, Phosphorus Radioisotopes, Iodine, Poliomyelitis
Published
1961

22. [Specific prevention of measles in Rumania. V. Studies on an attenuated live measles virus vaccine prepared at the Stefan S. Nicolau Institute of Virology and administered in various ways]

Author

N, Cajal, M, Cepleanu, C, Cernesc, Y, Sorodoc, N, Tigăeru, S, Biberi-Moroianu, D, Cuciu, G, Brebenel, E, Vucu, and A, Petrescu

Subjects
Evaluation Studies as Topic, Romania, Child, Preschool, Measles Vaccine, Vaccination, Humans, Infant, Child, Vaccines, Attenuated, Antibodies, Measles
Published
1973

28. [Immunoserological research in Hodgkin's disease]

Author

A, Schächter, D, Sărăţeanu, M, Cepleanu, N, Cajal, and I, Sorodoc

Subjects
Antibody Formation, Complement Fixation Tests, Animals, Humans, Chick Embryo, Antigens, Chlamydia, Hodgkin Disease, Skin Tests
Published
1966

40. The specific prevention of measles in Romania. Study of a vaccine prepared in human diploid cells

Author

M, Cepleanu, N, Cajal, C, Cernescu, Y, Sorodoc, I, Aderca, N, Tigăeru, D, Suciu, S, Manu, A, Ferra, S, Biberimoroianu, A, Dobrescu, and M, Lucian

Subjects
Fever, Romania, Injections, Subcutaneous, Measles Vaccine, Vaccination, Hemagglutinins, Viral, Infant, Hemagglutination Tests, Antibodies, Viral, Vaccines, Attenuated, Cell Line, Placebos, Measles virus, Child, Preschool, Humans, Child, Lung, Measles
Published
1973

41. [ETIOLOGICAL INVESTIGATIONS IN MALIGNANT LYMPHOGRANULOMATOSIS (HODGKIN'S DISEASE). VI. ATTEMPTED LOCALIZATION OF THE INTRACELLULAR BIOSYNTHESIS OF THE VIRUS BY MEANS OF ACRIDINE-ORANGE FLUORESCENT STAINING]

Author

N, CAJAL, M, CEPLEANU, S, MIHAIL, V, LOTREANU, and Y, SORODOC

Subjects
Tissue Culture Techniques, Cytoplasm, Microscopy, Microscopy, Electron, Virus Cultivation, Staining and Labeling, DNA, Viral, Acridines, Electrons, DNA, Coloring Agents, Oncogenic Viruses, Hodgkin Disease
Published
1963

48. [Studies of specific measles prevention]

Author

N, Cajal, M, Cepleanu, Y, Sorodoc, C, Cernescu, S, Manu, B, Weintraub, M, Lucian, and M, Popescu

Subjects
Adult, Adolescent, Romania, Child, Preschool, Measles Vaccine, Vaccination, Humans, Infant, Hemagglutination Inhibition Tests, Child, Antibodies, Immunization Schedule, Measles
Published
1970

Catalog

Books, media, physical & digital resources
See catalog results