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1. French guidelines on the use of systemic treatments for moderate‐to‐severe psoriasis in adults

Author

A.P. Villani, Bernard Guillot, Marie Tauber, M. Viguier, Florent Amatore, Centre Hospitalier Universitaire de Reims (CHU Reims), Immuno-Régulation dans les Maladies Auto-Immunes Inflammatoires et le Cancer - EA 7509 (IRMAIC), Université de Reims Champagne-Ardenne (URCA), Service de dermatologie, vénéreologie et cancérologie cutanée [Hôpital de la Timone - APHM], Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL), Centre de Recherche sur la Peau [Toulouse], Hôpital Robert Debré, Hôpital Robert Debré-Centre Hospitalier Universitaire de Reims (CHU Reims), Centre de Preuves en Dermatologie, and Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)

Subjects
Adult, medicine.medical_specialty, medicine.medical_treatment, MEDLINE, Guidelines and Position Statements, MESH: Algorithms, Dermatologic Agents/therapeutic use, Psoriasis/drug therapy, Severity of Ilness Index, Dermatology, Guidelines, 030207 dermatology & venereal diseases, 03 medical and health sciences, Psoriatic arthritis, 0302 clinical medicine, [SDV.SP.MED]Life Sciences [q-bio]/Pharmaceutical sciences/Medication, Psoriasis, Severity of illness, medicine, Humans, [INFO]Computer Science [cs], Intensive care medicine, Therapeutic strategy, 030203 arthritis & rheumatology, business.industry, Moderate to severe psoriasis, Antibodies, Monoclonal, medicine.disease, Comorbidity, 3. Good health, Infectious Diseases, PUVA therapy, business, [SDV.MHEP.DERM]Life Sciences [q-bio]/Human health and pathology/Dermatology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience; These guidelines were developed by the psoriasis research group of the French Society of Dermatology with the aim of providing updated decision-making algorithms for the systemic treatment of adult patients with moderate-to-severe psoriasis. Our algorithms were generated after rigorous evaluation of existing guidelines on the treatment of psoriasis and of publications concerning new systemic treatments, not yet incorporated into existing guidelines. A total of nine existing guidelines and 53 publications related to new systemic treatments were found to meet our criteria for use in the generation of the algorithms. We have proposed two new algorithms to assess therapeutic responses, both of which incorporate emerging criteria for evaluating treatment goals. Updated therapeutic strategy algorithms, incorporating both established and new systemic therapies, were also generated for the treatment of plaque psoriasis and psoriatic arthritis, together with recommendations for the treatment of particular forms of psoriasis and treatment of patients with comorbidities.

Published
2019
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2. Physiological stress monitoring using sodium ion potentiometric microsensors for sweat analysis

Author

Pierre Temple-Boyer, Frédéric Revol-Cavalier, Jean-Michel Lagarde, Gustave Savourey, Arnaud Cazalé, William Sant, Jean-Claude Launay, Frédéric Ginot, Jérôme-C. Launay, Didier Heinry, Équipe MICrosystèmes d'Analyse (LAAS-MICA), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), HEMODIA, CRSSA, Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre de Recherche sur la Peau [Toulouse], Paul Boyé Technologies, Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), and Université Fédérale Toulouse Midi-Pyrénées

Subjects
potentiometric sensor, Sodium, Potentiometric titration, Analytical chemistry, chemistry.chemical_element, 02 engineering and technology, 01 natural sciences, sweat analysis, Ion, Sweat analysis, ISE, Materials Chemistry, Potentiometric sensor, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Electrical and Electronic Engineering, Instrumentation, Physiological stress, physiological stress, 010401 analytical chemistry, Metals and Alloys, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, chemistry, Na+ ion detection, Electrode, ISFET, 0210 nano-technology
Abstract

International audience; In the frame of sweat analysis, two technologies, based on either ISE or ISFET devices, were developed for the implementation of pNa potentiometric microsensors. Both of them demonstrated good sodium ion Na+ detection properties with a global sensitivity of around 110 mV/pNa in NaCl-based solutions due to the use of an integrated "Ag/AgCl ink" pseudo-reference electrode. Then, in order to deal with in-vivo analysis of sweat natremia, a physiological sweatband prototype was developed, consisting of pNa-ISE and pNa-ISFET electronic detection modules as well as a textile-based sweat pump. Finally, sweating process was studied during series of experiments on twenty-five healthy consenting subjects. The sodium ion concentration [Na+] was successfully monitored in sweat during various heat exposures, demonstrating a global increase with exercise trial duration. Furthermore, a strong correlation was found between the sweat [Na+] concentration and the subject's internal temperature θ, allowing monitoring the subject's heat stress state. All in all, the relevance of the Na+ ion analysis was demonstrated for the physiological stress monitoring and pNa potentiometric microsensors were shown to be very promising for the development of smart sweatbands.

Published
2016
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3. Raman characterization of human skin aging

Author

Gwendal Josse, Anne-Marie Schmitt, Eléonore Gravier, Emmanuel Questel, Aurélie Villaret, Tuvana Satar, Célia Ipinazar, Valérie Samouillan, Florence Nadal, Céline Mias, Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut National Polytechnique de Toulouse - INPT (FRANCE), Laboratoires Pierre Fabre (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Centre Interuniversitaire de Recherche et d'Ingénierie des Matériaux - CIRIMAT (Toulouse, France), Centre de Recherche sur la Peau [Toulouse], Centre interuniversitaire de recherche et d'ingenierie des matériaux (CIRIMAT), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC), and Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)

Subjects
Adult, Aging, Proline, Biopsy, Photoaging, Biochimie, Matériaux, Human skin, Dermatology, Matrix (biology), Spectrum Analysis, Raman, 01 natural sciences, [SPI.MAT]Engineering Sciences [physics]/Materials, Skin Aging, 010309 optics, Young Adult, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Dermis, 0103 physical sciences, medicine, Humans, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Glycosaminoglycans, integumentary system, Chemistry, Hydration state, Papillary dermis, Middle Aged, medicine.disease, Forearm, Hydroxyproline, medicine.anatomical_structure, Glycosaminoglycan, Reticular connective tissue, Raman spectroscopy, Biophysics, Buttocks, Female, Collagen, Reticular Dermis
Abstract

International audience; Skin aging is a complex biological process mixing intrinsic and extrinsic factors, such as sun exposure. At the molecular level, skin aging affects in particular the extracellular matrix proteins. Materials and Methods: Using Raman imaging, which is a nondestructive approach appropriate for studying biological samples, we analyzed how aging modifies the matrix proteins of the papillary and reticular dermis. Biopsies from the buttock and dorsal forearm of volunteers younger than 30 and older than 60 were analyzed in order to identify chronological and photoaging processes. Analyses were performed on skin section, and Raman spectra were acquired separately on the different dermal layers. Results: We observed differences in dermal matrix structure and hydration state with skin aging. Chronological aging alters in particular the collagen of the papillary dermis, while photoaging causes a decrease in collagen stability by altering proline and hydroxyproline residues in the reticular dermis. Moreover, chronological aging alters glycosaminoglycan content in both dermal compartments. Conclusion: Alterations of the papillary and reticular dermal matrix structures during photo-and chronological aging were clearly depicted by Raman spectroscopy.

Published
2018
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4. Characterisation of Cutibacterium acnes phylotypes in acne and in vivo exploratory evaluation of Myrtacine®

Author

Christine Roques, Amir Khammari, Brigitte Dréno, T. Nocera, C. Peraud, Sophie Pécastaings, Valérie Mengeaud, Laboratoire de génie chimique [ancien site de Basso-Cambo] (LGC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, FONdation pour le DEveloppement de la REcherche PHARmaceutique - FONDEREPHAR (FRANCE), Service de Bactériologie-Hygiène [CHU Toulouse] (Hôpital Purpan), CHU Toulouse [Toulouse], Centre de Recherche Clinique sur la Peau [Toulouse] (Clinical Skin Research Center), Pierre Fabre Dermo-Cosmetique [Toulouse], Laboratoires Dermatologiques Ducray [Les Cauquillous, Lavaur], Pierre Fabre Dermo-Cosmétique [Les Cauquillous, Lavaur], Clinical and Translational Research in Skin Cancer (CRCINA-ÉQUIPE 2), Centre de Recherche en Cancérologie et Immunologie Nantes-Angers (CRCINA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA), This study was supported by Pierre Fabre Dermo-Cosmetique, DUCRAY Laboratoires Dermatologiques, Lavaur, France., Bernardo, Elizabeth, Service Bactériologie et hygiène [CHU Toulouse], Institut Fédératif de Biologie (IFB), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Biologie [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Centre de Recherche sur la Peau [Toulouse], Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes), Centre Hospitalier Universitaire de Nantes - CHU Nantes (FRANCE), Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut National Polytechnique de Toulouse - INPT (FRANCE), Institut National de la Santé et de la Recherche Médicale - INSERM (FRANCE), Laboratoires Pierre Fabre (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Université de Nantes (FRANCE), FONdation pour le DEveloppement de la REcherche PHARmaceutique- FONDEREPHAR (FRANCE), Centre Hospitalier Universitaire de Toulouse - CHU Toulouse (FRANCE), Université Nantes Angers Le Mans - UNAM (FRANCE), Laboratoire de Pharmacochimie Pierre Fabre Dermo-Cosmétique (Toulouse, France), Centre de recherche en cancérologie et immunologie Nantes-Angers - CRCINA (Nantes, France), and Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)

Subjects
0301 basic medicine, Adult, Male, medicine.medical_specialty, Adolescent, medicine.drug_class, 030106 microbiology, Antibiotics, Erythromycin, [SDV.CAN]Life Sciences [q-bio]/Cancer, Dermatology, Drug resistance, Severity of Illness Index, Phylotypes, Macrolide Antibiotics, 03 medical and health sciences, Young Adult, Hyperproliferation, [SDV.CAN] Life Sciences [q-bio]/Cancer, Severity of illness, Acne Vulgaris, medicine, Génie chimique, Humans, Propionibacterium acnes, Génie des procédés, Acne, business.industry, Plant Extracts, Case-control study, Clindamycin, medicine.disease, Bacterial Load, 3. Good health, Infectious Diseases, Case-Control Studies, Biologie cellulaire, Female, Cutibacterium acnes, business, medicine.drug
Abstract

International audience; Objective Our main objective was to compare Cutibacterium acnes (C. acnes) skin colonisation in patients with mild to moderate acne versus healthy controls and secondly, to evaluate a Myrtacine® -based cream on C. acnes total population and antibioresistant Cutibacteria in patients with acne.Methods In 60 acne patients (Global Acne Severity Scale, GEA grades 2–3), of mean age 20 [15–30] years and in 24 age-and sex-matched healthy controls, forehead strips samplings were performed for microbiological analysis of come-dones by colony forming unit (CFU) counts of global C. acnes and erythromycin (EryR) or clindamycin-resistant (ClnR) populations of Cutibacterium and determination of phylotypes by MALTI-TOF. Clinical evaluations of acne patients (GEA, lesion count, porphyrin fluorescence) were performed at baseline and after 56 days of twice-daily application of a Myrtacine® -based cream.Results We first showed (i) high and similar levels of C. acnes colonisation in superficial pilosebaceous follicles and detection of EryR and ClnR strains in both acne and control groups; (ii) different repartition of phylotypes in acne patients versus healthy control, with a predominance of phylotype IA in acne patients and a link between phylotype IA and erythromycin resistance. Besides, after treatment with the Myrtacine® -based cream in acne patients, there was no change in C. acnes total load, but a significant decrease of EryR Cutibacteria, reduced porphyrin production by C. acnes, a decrease in acne severity (GEA), associated with reduced retentional and inflammatory lesions.Conclusion Cutibacterium acnes colonisation was not significantly different in acne versus control groups. Phylotype IA was predominant in acne patient and in EryR C. acnes. A Myrtacine® -based cream significantly reduced the level of EryR Cutibacteria in vivo and improved acne lesions.

Published
2018
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5. Prevention of skin flap necrosis by estradiol involves reperfusion of a protected vascular network

Author

Jean-François Arnal, Pierre Gourdy, Céline E. Toutain, Hortense Berges, Patricia Vicendo, Julie Favre, Andrée Krust, Anne-Marie Schmitt, Marie-José Fouque, Isabelle Raymond-Letron, Laurent Brouchet, Pierre Chambon, Françoise Lenfant, Institut de médecine moléculaire de Rangueil (I2MR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-IFR150-Institut National de la Santé et de la Recherche Médicale (INSERM), Département d'Anatomie Pathologique, Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Interactions moléculaires et réactivité chimique et photochimique (IMRCP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Centre de Recherche sur la Peau [Toulouse], Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées- Institut Fédératif de Recherche Bio-médicale Institution (IFR150)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Chimie de Toulouse (ICT-FR 2599), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Simon, Marie Francoise, Université de Toulouse (UT)-Université de Toulouse (UT)- Institut Fédératif de Recherche Bio-médicale Institution (IFR150)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT), Institut de Chimie de Toulouse (ICT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Fédération de Recherche Fluides, Energie, Réacteurs, Matériaux et Transferts (FERMAT), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Time Factors, Necrosis, Physiology, Dermatologic Surgical Procedures, MESH: Mice, Knockout, Surgical Flaps, MESH: Drug Implants, MESH: Dose-Response Relationship, Drug, Brain ischemia, Mice, chemistry.chemical_compound, 0302 clinical medicine, Ischemia, MESH: Animals, MESH: Bone Marrow Transplantation, MESH: Estrogen Receptor alpha, Bone Marrow Transplantation, Skin, Drug Implants, Mice, Knockout, 0303 health sciences, Estradiol, integumentary system, MESH: Selective Estrogen Receptor Modulators, Vascular endothelial growth factor, MESH: Cell Survival, 030220 oncology & carcinogenesis, Ovariectomized rat, Female, MESH: Estradiol, medicine.symptom, Cardiology and Cardiovascular Medicine, Selective Estrogen Receptor Modulators, medicine.medical_specialty, Cell Survival, medicine.drug_class, Ovariectomy, Mice, Nude, MESH: Ovariectomy, MESH: Mice, Hairless, 03 medical and health sciences, MESH: Skin, In vivo, MESH: Mice, Inbred C57BL, Internal medicine, medicine, MESH: Mice, Nude, Animals, MESH: Mice, 030304 developmental biology, MESH: Necrosis, Mice, Hairless, Dose-Response Relationship, Drug, business.industry, MESH: Time Factors, Estrogen Receptor alpha, MESH: Surgical Flaps, medicine.disease, Mice, Inbred C57BL, Disease Models, Animal, Tamoxifen, Endocrinology, chemistry, Regional Blood Flow, Estrogen, MESH: Regional Blood Flow, MESH: Tamoxifen, MESH: Disease Models, Animal, MESH: Ischemia, business, MESH: Female, Ex vivo
Abstract

Although 17β-estradiol (E2) is protective in experimental models of myocardial and brain ischemia, its effect on skin ischemia remains unknown. Here, we assessed the protective effect of E2 in a mouse model of skin ischemia, mimicking the surgery of skin flaps. Whereas necrosis appeared in the half portion of the skin flap within 1 week after surgery in ovariectomized mice, it was reduced up to 10-fold when mice were pretreated with E2, at least 3 days before the surgery. The beneficial effect of E2 appeared to be attributable to an increase in skin survival, revealed by measuring viability of ex vivo explants and enhancement of the antiapoptotic Bcl-2 protein expression in vivo. This protective effect on the skin contributed to the protection of the vascular network and facilitated reperfusion, which was found to be accelerated in ovariectomized E2-treated mice, whereas hemorrhages were observed in untreated mice. E2 also increased expression of fibroblast growth factor-2 isoforms in the skin and circulating vascular endothelial growth factor in the serum. Finally, this protective effect of E2 was abolished in estrogen receptor–deficient mice (ERα −/− ) but maintained in chimeric mice reconstituted with ERα-deficient bone marrow, indicating dispensable action of E2 in bone marrow–derived cells. This protective effect of E2 was mimicked by treatment with tamoxifen, a selective estrogen receptor modulator. In conclusion, we have demonstrated for the first time that E2 exerts a major preventive effect of skin flap necrosis through a prevention of ischemic-induced skin lesions, including those of the vascular network, which contributes to accelerate the reperfusion of the skin flap.

Published
2009
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7. Protection against summer solar lentigo over-pigmentation with a SPF30 daily cream.

Author

Josse G, Le Digabel J, and Questel E

Subjects
Aged, Female, Hand Dermatoses drug therapy, Humans, Hyperpigmentation complications, Lentigo complications, Microscopy, Confocal, Middle Aged, Color, Hand Dermatoses prevention & control, Hyperpigmentation prevention & control, Lentigo drug therapy, Seasons, Skin Cream therapeutic use, Sunscreening Agents therapeutic use
Abstract

Background/purpose: The aim of this study was to measure lentigines' pigmentation over a long period of time and evaluate if summer over-pigmentation can be avoided by the use a SPF30 day skin cream., Methods: Seventeen healthy female volunteers aged 50 and over and presenting lentigines participated in the study from spring to summer. Throughout the study, all subjects applied a SPF30 daily skin cream to only one hand. Color measurements of the target lesions were performed with a chromameter and with a color-calibrated camera. Target lesions were also imaged with in vivo reflectance confocal microscopy (RCM). A specific procedure for re-registering the images was developed to ensure that the same papillae were measured over time., Results: Both color measurement methods, chromametry and color-calibrated camera, showed that lentigines treated over time with the SPF30 day skin cream were significantly lighter than the non-treated lentigines. The RCM images showed a decrease in the papillary contrast for the treated lentigines., Conclusion: This study shows that this over-pigmentation can be avoided using a SPF30 day skin cream. Moreover, we have demonstrated that very fine re-registration of the RCM images is possible and ensures a more robust analysis., (© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

Published
2018
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