1. Osteo-modulatory potential of biologically synthesized cis-resveratrol passivated gold nanoparticles.
- Author
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Gupta A, Madhyastha H, Kumar A, and Singh S
- Subjects
- Animals, Mice, Cell Line, Alkaline Phosphatase metabolism, Resveratrol administration & dosage, Resveratrol pharmacology, Resveratrol chemistry, Gold chemistry, Metal Nanoparticles chemistry, Metal Nanoparticles administration & dosage, Osteoblasts drug effects, Osteoblasts metabolism, Osteogenesis drug effects, Cell Movement drug effects, Cell Differentiation drug effects, Cell Proliferation drug effects
- Abstract
Resveratrol, a stilbene, particularly trans-isomer, shows significant osteogenic potential but experiences high instability and poor bioavailability. However, cis-isomer (cRes) is not explored yet due to its instability. Our study investigates the osteoinductive potential of cRes for the first time by stabilizing it onto the surface of gold nanoparticles. cRes capped GNPs (cRGNPs) presented no toxic effects on the MC3T3-E1 cells with increased levels of alkaline phosphatase and calcium deposition. The nanoparticles presented a 2.6-fold increase in cell number compared to the control. The pro-migratory effect of the cRGNPs was also significantly higher (97.21 ± 0.99 % migration) in 4 days. The osteoinductivity was further confirmed by enhanced expression of osteoblastic genes like RUNX2, OPN, OCN, BMP, OPG, and Col1A. The stability provided to cRes upon conjugating to GNPs allowed exploration of its potential in aiding proliferation, migration, and differentiation of the pre-osteoblasts, which will be beneficial in repairing bone defects., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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