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364 results on '"Cecchelli, Roméo"'

3. Evaluation of drug-induced neurotoxicity based on metabolomics, proteomics and electrical activity measurements in complementary CNS in vitro models

Author

Schultz, Luise, Zurich, Marie-Gabrielle, Culot, Maxime, da Costa, Anaelle, Landry, Christophe, Bellwon, Patricia, Kristl, Theresa, Hörmann, Katrin, Ruzek, Silke, Aiche, Stephan, Reinert, Knut, Bielow, Chris, Gosselet, Fabien, Cecchelli, Romeo, Huber, Christian G., Schroeder, Olaf H.-U., Gramowski-Voss, Alexandra, Weiss, Dieter G., and Bal-Price, Anna

Published
2015
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19. Modelling the Blood–Brain Barrier

Author

Dehouck, Marie-Pierre, primary, Vandenhaute, Elodie, additional, Dehouck, Lucie, additional, Sevin, Emmanuel, additional, Lenfant, Anne-Marie, additional, Delplace, Yannick, additional, Hallier-Vanuxeem, Dorothée, additional, Culot, Maxime, additional, and Cecchelli, Roméo, additional

Published
2011
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22. Modeling the Blood-Brain Barrier

Author

Cecchelli, Roméo, primary, Coisne, Caroline, additional, Dehouck, Lucie, additional, Miller, Florence, additional, Dehouck, Marie-Pierre, additional, Buée-Scherrer, Valérie, additional, and Dehouck, Bénédicte, additional

Published
2007
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37. High transcytosis of melanotransferrin (P97) across the blood–brain barrier

Author

Demeule, Michel, Poirier, Julie, Jodoin, Julie, Bertrand, Yanick, Desrosiers, Richard R., Dagenais, Claude, Nguyen, Tran, Lanthier, Julie, Gabathuler, Reinhard, Kennard, Malcolm, Jefferies, Wilfred A., Karkan, Delara, Tsai, Sam, Fenart, Laurence, Cecchelli, Roméo, and Béliveau, Richard

Published
2002

46. Brain pericytes from stress-susceptible pigs increase blood-brain barrier permeability in vitro

Author

Vandenhaute Elodie, Culot Maxime, Gosselet Fabien, Dehouck Lucie, Godfraind Catherine, Franck Michel, Plouët Jean, Cecchelli Roméo, Dehouck Marie-Pierre, and Ruchoux Marie-Magdeleine

Subjects
Blood–brain barrier, Endothelial permeability, Neurovascular unit, Pericytes, Perivascular spaces, Porcine stress syndrome, Neurology. Diseases of the nervous system, RC346-429
Abstract

Abstract Background The function of pericytes remains questionable but with improved cultured technique and the use of genetically modified animals, it has become increasingly clear that pericytes are an integral part of blood–brain barrier (BBB) function, and the involvement of pericyte dysfunction in certain cerebrovascular diseases is now emerging. The porcine stress syndrome (PSS) is the only confirmed, homologous model of malignant hyperthermia (MH) in veterinary medicine. Affected animals can experience upon slaughter a range of symptoms, including skeletal muscle rigidity, metabolic acidosis, tachycardia and fever, similar to the human syndrome. Symptoms are due to an enhanced calcium release from intracellular stores. These conditions are associated with a point mutation in ryr1/hal gene, encoding the ryanodine receptor, a calcium channel. Important blood vessel wall muscle modifications have been described in PSS, but potential brain vessel changes have never been documented in this syndrome. Methods In the present work, histological and ultrastructural analyses of brain capillaries from wild type and ryr1 mutated pigs were conducted to investigate the potential impairment of pericytes, in this pathology. In addition, brain pericytes were isolated from the three porcine genotypes (wild-type NN pigs; Nn and nn pigs, bearing one or two (n) mutant ryr1/hal alleles, respectively), and tested in vitro for their influence on the permeability of BBB endothelial monolayers. Results Enlarged perivascular spaces were observed in ryr1-mutant samples, corresponding to a partial or total detachment of the astrocytic endfeet. These spaces were electron lucent and sometimes filled with lipid deposits and swollen astrocytic feet. At the ultrastructural level, brain pericytes did not seem to be affected because they showed regular morphology and characteristics, so we aimed to check their ability to maintain BBB properties in vitro. Our results indicated that pericytes from the three genotypes of pigs had differing influences on the BBB. Unlike pericytes from NN pigs, pericytes from Nn and nn pigs were not able to maintain low BBB permeability. Conclusions Electron microscopy observations demonstrated brain capillary modifications in PSS condition, but no change in pericyte morphology. Results from in vitro experiments suggest that brain pericytes from ryr1 mutated pigs, even if they are not affected by this condition at the ultrastructural level, are not able to maintain BBB integrity in comparison with pericytes from wild-type animals.

Published
2012
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47. A large-scale electrophoresis- and chromatography-based determination of gene expression profiles in bovine brain capillary endothelial cells after the re-induction of blood-brain barrier properties

Author

Duban-Deweer Sophie, Deracinois Barbara, Pottiez Gwënaël, Cecchelli Roméo, Fenart Laurence, Karamanos Yannis, and Flahaut Christophe

Subjects
Cytology, QH573-671
Abstract

Abstract Background Brain capillary endothelial cells (BCECs) form the physiological basis of the blood-brain barrier (BBB). The barrier function is (at least in part) due to well-known proteins such as transporters, tight junctions and metabolic barrier proteins (e.g. monoamine oxidase, gamma glutamyltranspeptidase and P-glycoprotein). Our previous 2-dimensional gel proteome analysis had identified a large number of proteins and revealed the major role of dynamic cytoskeletal remodelling in the differentiation of bovine BCECs. The aim of the present study was to elaborate a reference proteome of Triton X-100-soluble species from bovine BCECs cultured in the well-established in vitro BBB model developed in our laboratory. Results A total of 215 protein spots (corresponding to 130 distinct proteins) were identified by 2-dimensional gel electrophoresis, whereas over 350 proteins were identified by a shotgun approach. We classified around 430 distinct proteins expressed by bovine BCECs. Our large-scale gene expression analysis enabled the correction of mistakes referenced into protein databases (e.g. bovine vinculin) and constitutes valuable evidence for predictions based on genome annotation. Conclusions Elaboration of a reference proteome constitutes the first step in creating a gene expression database dedicated to capillary endothelial cells displaying BBB characteristics. It improves of our knowledge of the BBB and the key proteins in cell structures, cytoskeleton organization, metabolism, detoxification and drug resistance. Moreover, our results emphasize the need for both appropriate experimental design and correct interpretation of proteome datasets.

Published
2010
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