7 results on '"Ceccato CM"'
Search Results
2. Evaluation of Serum CRP as a Risk Factor for Incident Asthma in Adults.
- Author
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Venker, C, primary, Ceccato, CM, additional, Sherrill, DL, additional, Halonen, M, additional, Martinez, FD, additional, and Guerra, S, additional
- Published
- 2009
- Full Text
- View/download PDF
3. Phenotypic and functional properties of Helios+ regulatory T cells.
- Author
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Zabransky DJ, Nirschl CJ, Durham NM, Park BV, Ceccato CM, Bruno TC, Tam AJ, Getnet D, and Drake CG
- Subjects
- Animals, Antigens, CD genetics, Gene Expression Regulation, Neoplastic, Glucocorticoid-Induced TNFR-Related Protein genetics, Integrin alpha Chains genetics, Mice, Mice, Inbred BALB C, Neoplasms genetics, Phenotype, Spleen cytology, T-Lymphocytes, Regulatory cytology, Up-Regulation, DNA-Binding Proteins genetics, T-Lymphocytes, Regulatory metabolism, Transcription Factors genetics
- Abstract
Helios, an Ikaros family transcription factor, is preferentially expressed at the mRNA and protein level in regulatory T cells. Helios expression previously appeared to be restricted to thymic-derived Treg. Consistent with recent data, we show here that Helios expression is inducible in vitro under certain conditions. To understand phenotypic and functional differences between Helios(+) and Helios(-) Treg, we profiled cell-surface markers of FoxP3(+) Treg using unmanipulated splenocytes. We found that CD103 and GITR are expressed at high levels on a subset of Helios(+) Treg and that a Helios(+) Treg population could be significantly enriched by FACS sorting using these two markers. Quantitative real-time PCR (qPCR) analysis revealed increased TGF-β message in Helios(+) Treg, consistent with the possibility that this population possesses enhanced regulatory potential. In tumor-bearing mice, we found that Helios(+) Treg were relatively over-represented in the tumor-mass, and BrdU studies showed that, in vivo, Helios(+) Treg proliferated more than Helios(-) Treg. We hypothesized that Helios-enriched Treg might exert increased suppressive effects. Using in vitro suppression assays, we show that Treg function correlates with the absolute number of Helios(+) cells in culture. Taken together, these data show that Helios(+) Treg represent a functional subset with associated CD103 and GITR expression.
- Published
- 2012
- Full Text
- View/download PDF
4. HIV type 1-mediated downregulation of HLA-B*57/B*5801 proteins on elite suppressor CD4+ T cells.
- Author
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Sampah ME, Ceccato CM, and Blankson JN
- Subjects
- Alleles, Humans, CD4-Positive T-Lymphocytes metabolism, Down-Regulation, HIV-1 physiology, HLA-B Antigens metabolism
- Abstract
Elite controllers or suppressors (ES) are HIV-1-infected patients who maintain viral loads of <50 copies/ml without antiretroviral therapy. While HLA-B*57 and B*5801 alleles are overrepresented in ES, many HLA-B*57/B*5801 patients become chronic progressors (CP). We show here that HIV-1 infection results in similar levels of downregulation of HLA-B*57 and HLA-B*5801 molecules on primary CD4(+) T cells from ES and CP. Thus, differences in HIV-1-mediated downregulation of HLA-B*57/B*5801 molecules do not distinguish ES from CP.
- Published
- 2011
- Full Text
- View/download PDF
5. HIV-1 Gag evolution in recently infected human leukocyte antigen-B*57 patients with low-level viremia.
- Author
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Durand CM, O'Connell KA, Apuzzo LG, Langan SJ, Imteyaz H, Ahonkhai AA, Ceccato CM, Williams TM, Margolick JB, and Blankson JN
- Subjects
- HIV Infections immunology, HLA-B Antigens immunology, Humans, Molecular Sequence Data, Mutation, Epitopes, T-Lymphocyte genetics, Genes, gag genetics, HIV Infections genetics, HIV-1 genetics, HLA-B Antigens genetics
- Abstract
We studied viral evolution in five human leukocyte antigen (HLA)-B*57 patients recently infected with HIV-1. Escape mutations in HLA-B*57-restricted Gag epitopes were present at study entry in all patients, but were not associated with significant increases in viremia. Conversely, no new escape mutations in HLA-B*57-restricted epitopes or known compensatory mutations were detected in patients who experienced significant increases in viremia. Thus, the development of escape mutations alone does not determine virologic outcome in recently infected HLA-B*57 patients.
- Published
- 2010
- Full Text
- View/download PDF
6. Morbidity and mortality associated with the restrictive spirometric pattern: a longitudinal study.
- Author
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Guerra S, Sherrill DL, Venker C, Ceccato CM, Halonen M, and Martinez FD
- Subjects
- Adult, Aged, Aged, 80 and over, Arizona epidemiology, Asthma epidemiology, Epidemiologic Methods, Female, Forced Expiratory Volume, Humans, Immunoglobulin E blood, Lung Diseases mortality, Lung Diseases physiopathology, Lung Diseases, Obstructive epidemiology, Lung Diseases, Obstructive mortality, Lung Diseases, Obstructive physiopathology, Male, Middle Aged, Recurrence, Sex Factors, Smoking adverse effects, Smoking epidemiology, Spirometry, Vital Capacity, Young Adult, Lung Diseases epidemiology
- Abstract
Background: Recent studies have suggested that a restrictive pattern assessed with a single spirometric test is associated with increased morbidity and mortality. This study was undertaken to determine demographic, clinical and mortality profiles of subjects with either a recurrent or an inconsistent restrictive spirometric pattern assessed prospectively., Methods: Data from 2048 adult participants in the population-based TESAOD study were analysed. Normal (forced expiratory volume in 1 s/forced vital capacity (FEV(1)/FVC) ratio >or=70% and FVC >or=80% predicted), restrictive (FEV(1)/FVC >or=70% and FVC <80% predicted) and obstructive (FEV(1)/FVC <70%) patterns were assessed at the enrollment survey in 1972 and in 11 subsequent follow-up surveys up to 1996. Demographic and clinical characteristics were measured at enrollment and vital status and cause of death were assessed at January 2005., Results: Overall, 12% of participants had a restrictive spirometric pattern at enrollment. They were less likely to be male, to smoke and to have asthma, and had lower IgE levels than subjects in the obstructive group. Among subjects with a restrictive pattern at enrollment, 38% developed an obstructive pattern during follow-up. The remaining 62% had either a recurrent (restrictive pattern >or=50% of follow-up surveys) or inconsistent (restrictive pattern <50% of follow-up surveys) longitudinal restrictive pattern. The recurrent and inconsistent restrictive groups had increased mortality risk for all-cause (adjusted HR 1.7 (95% CI 1.3 to 2.3) and 1.9 (95% CI 1.4 to 2.6), respectively), heart disease (2.0 (95% CI 1.3 to 3.1) and 2.7 (95% CI 1.7 to 4.3)), stroke (2.4 (95% CI 0.9 to 6.3) and 3.5 (95% CI 1.2 to 9.8)) and diabetes (8.0 (95% CI 2.9 to 21.8) and 6.0 (95% CI 1.9 to 19.2))., Conclusions: The restrictive spirometric pattern identifies a pulmonary condition that is distinguishable from obstructive lung disease and is associated with an increased risk of life-threatening comorbidities.
- Published
- 2010
- Full Text
- View/download PDF
7. Chronic bronchitis before age 50 years predicts incident airflow limitation and mortality risk.
- Author
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Guerra S, Sherrill DL, Venker C, Ceccato CM, Halonen M, and Martinez FD
- Subjects
- Adult, Age of Onset, Aged, Aged, 80 and over, Airway Obstruction blood, Airway Obstruction physiopathology, Bronchitis, Chronic blood, Bronchitis, Chronic physiopathology, C-Reactive Protein metabolism, Chronic Disease, Cough mortality, Cough physiopathology, Female, Forced Expiratory Volume physiology, Humans, Interleukin-8 metabolism, Male, Middle Aged, Risk Factors, Sputum metabolism, Vital Capacity physiology, Young Adult, Airway Obstruction mortality, Bronchitis, Chronic mortality
- Abstract
Background: Previous studies on the relationship of chronic bronchitis to incident airflow limitation and all-cause mortality have provided conflicting results, with positive findings reported mainly by studies that included populations of young adults. This study sought to determine whether having chronic cough and sputum production in the absence of airflow limitation is associated with onset of airflow limitation, all-cause mortality and serum levels of C-reactive protein (CRP) and interleukin-8 (IL-8), and whether subjects' age influences these relationships., Methods: 1412 participants in the long-term Tucson Epidemiological Study of Airway Obstructive Disease who at enrolment (1972-1973) were 21-80 years old and had FEV(1)/FVC (forced expiratory volume in 1 s/forced vital capacity) > or = 70% and no asthma were identified. Chronic bronchitis was defined as cough and phlegm production on most days for > or = 3 months in two or more consecutive years. Incidence of airflow limitation was defined as the first follow-up survey with FEV(1)/FVC <70%. Serum IL-8 and CRP levels were measured in cryopreserved samples from the enrolment survey., Results: After adjusting for covariates, chronic bronchitis at enrolment significantly increased the risk for incident airflow limitation and all-cause mortality among subjects <50 years old (HR 2.2, 95% CI 1.3 to 3.8; and HR 2.2, 95% CI 1.3 to 3.8; respectively), but not among subjects > or = 50 years old (HR 0.9, 95% CI 0.6 to 1.4; and HR 1.0, 95% CI 0.7 to 1.3). Chronic bronchitis was associated with increased IL-8 and CRP serum levels only among subjects <50 years old., Conclusions: Among adults <50 years old, chronic bronchitis unaccompanied by airflow limitation may represent an early marker of susceptibility to the effects of cigarette smoking on systemic inflammation and long-term risk for chronic obstructive pulmonary disease and all-cause mortality.
- Published
- 2009
- Full Text
- View/download PDF
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