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The present work deals with the first record of ostracods in the Estiva Formation, Pernambuco Basin (Cupe, 1 LABIO-PE1 borehole). The Pernambuco Basin extends throughout the southern coast of the state of Pernambuco and is limited by the Pernambuco Shear Zone, in the north and by the Maragogi High, in the south. The Estiva Formation consists of continental shelf carbonates dated as Upper Cretaceous. The analysis and interpretation of the data were performed through literature review, stratigraphic data surveys, and sampling. The methodology used for the treatment of the samples consisted of the following steps: collection, weighing and fragmentation of the samples; washing and drying of the calcined materials; and screening and picking of the carbonatic microfossils. Rare ostracods were found, probably belonging to the brackish genus Fossocytheridea Swain & Brown, 1964. The recognition of the ostracod fauna and its paleoecology aims to contribute to the understanding of the Pernambuco Basin evolution.