Search

Hydroxyurea, an oral medication with important clinical benefits in the treatment of sickle cell anemia, can be accurately determined in plasma with a transition metal dichalcogenide-based electrochemical sensor. We used a two-dimensional molybdenum sulfide material (MoS 2 ) selectively electrodeposited on a polycrystalline gold electrode via tailored waveform polarization in the gold electrical double layer formation region. The electro-activity of the modified electrode depends on the electrical waveform parameters used to electro-deposit MoS 2 . The concomitant oxidation of the MoS 2 material during its electrodeposition allows for the tuning of the sensor's specificity. Chemometrics, utilizing mathematical procedures such as principal component analysis and multivariable partial least square regression, were used to process the electrochemical data generated at the bare and the modified electrodes, thus allowing the hydroxyurea concentrations to be predicted in human plasma. A limit-of-detection of 22 nM and a sensitivity of 37 nA cm -2 µM -1 were found to be suitable for pharmaceutical and clinical applications.

A method to assemble loaded stimuli-responsive DNA-polyacrylamide hydrogel-stabilized microcapsules is presented. The method involves coating substrate-loaded CaCO 3 microparticles, functionalized with nucleic acid promoter units, and cross-linking DNA-modified polyacrylamide chains on the microcapsules, using the hybridization chain reaction (HCR) to yield the DNA-cross-linked hydrogel coating. Dissolution of the CaCO 3 particles generated the substrate-loaded hydrogel-protected microcapsules. The microcapsule-hydrogel shells include engineered stimuli-responsive oligonucleotide cross-linkers that control the stiffness of the hydrogel shells, allowing the triggered release of the loads. One approach includes the incorporation of cofactor-dependent DNAzyme units into the cross-linked hydrogel layers (cofactor = Mg 2+ ions, Zn 2+ ions, or histidine) as stimuli-responsive units. Cleavage of the cross-linking DNAzyme substrates by the respective cofactors yields hydrogel coatings with a reduced stiffness and higher porosity that allow the release of the loads. A further approach involved the application of the HCR process to assemble the bilayer hydrogel microcapsules that are unlocked by two cooperative triggers. Bilayer microcapsules consisting of a K + ions-stabilized G-quadruplex/18-crown-6-ether (CE) responsive layer and a Mg 2+ ion DNAzyme-responsive layers are presented. Unlocking and locking of the G-quadruplex cross-linked layer by 18-crown-6-ether and K + ions, respectively, in the presence of Mg 2+ ions allow the switchable controlled release of the load. In addition, the intercommunication of two kinds of stimuli-responsive bilayer hydrogel microcapsules carrying two different loads (tetramethylrhodamine-dextran, TMR-D, and CdSe/ZnS quantum dots) is demonstrated. The intercommunication process involves the stimuli-triggered generation of "information transfer" strands from one microcapsule to another that activate the release of the loads.

Antipsychotic clozapine is the most effective medication currently available for schizophrenia. However, clozapine is dramatically underutilized due to its harsh side effects that are not effectively monitored. By continuously monitoring clozapine blood levels, such as use of an implantable glucometer, which has transformed diabetes management, the treatment can be optimized and side effects will be minimized. Currently, none of the methods for clozapine detection show the ability to repeatedly measure clozapine in whole blood without pretreatment steps. Here we propose using a microelectrode modified with reduced graphene oxide-a material that was used for repeatable measurements in implantable electrochemical devices. We present the successful direct electrodeposition of reduced-graphene oxide coating onto microelectrodes. Systematic characterization of the electrodeposition technique parameters (i.e., the technique scan rate and the number of cycles) revealed their effect on the electrochemical activity and the structural properties (the film thickness and roughness) of the films. The developed reduced-graphene oxide-modified microelectrode exhibited the feasibility to detect clozapine in microliters-volume-samples of whole blood with a limit-of-detection and a sensitivity of 0.64 ± 0.04 μM and 19.6 ± 1.3 μA/cm 2 μM, respectively. Moreover, the reduced graphene oxide-modified microelectrodes exhibited high repeatability (retaining 94.6% of the electrochemical signal after 10 repeats), reproducibility (3.6% relative standard deviation), and storage stability (retaining 89% of the electrochemical signal after 4 weeks). Finally, relative recovery studies of 0.5, 1, and 2 μM clozapine concentrations resulted in 108 ± 4.0%, 112 ± 3.5%, and 103 ± 2.2%, respectively. Future studies should investigate the microelectrode fouling mechanisms in whole blood and explore methods to overcome fouling., (Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.)

The vascular endothelial growth factor (VEGF) induces pathological angiogenetic ocular diseases. It is a scientific challenge to develop carriers for the controlled release of inhibitors for VEGF present in the back of the eye domain. Carbon dots (C-dots) functionalized with the VEGF aptamer are introduced and the hybrid nanoparticles are used for ocular nanomedicine. The C-dots are applied as effective carriers of the anti-VEGF aptamer across the cornea, yielding therapeutic levels upon topical administration. The hybrids show no toxicity for both in vitro and in vivo murine animal model, and further enable noninvasive intraocular concentration monitoring through the C-dots inherent fluorescence. In addition, the hybrid C-dots effectively inhibit VEGF-stimulated angiogenesis in choroidal blood vessels. This inhibition is comparable to two commercially available anti-VEGF drugs, bevacizumab and aflibercept. The hybrid aptamer-modified C-dots provide a versatile nanomaterial to treat age-related macular degeneration and diabetic retinopathy., (© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Zeolitic Zn 2+ -imidazolate cross-linked framework nanoparticles, ZIF-8 NMOFs, are used as "smart" glucose-responsive carriers for the controlled release of drugs. The ZIF-8 NMOFs are loaded with the respective drug and glucose oxidase (GOx), and the GOx-mediated aerobic oxidation of glucose yields gluconic acid and H 2 O 2 . The acidification of the NMOFs' microenvironment leads to the degradation of the nanoparticles and the release of the loaded drugs. In one sense-and-treat system, GOx and insulin are loaded in the NMOFs. In the presence of glucose, the nanoparticles are unlocked, resulting in the release of insulin. The release of insulin is controlled by the concentration of glucose. In the second sense-and-treat system, the NMOFs are loaded with the antivascular endothelial growth factor aptamer (VEGF aptamer) and GOx. In the presence of glucose, the ZIF-8 NMOFs are degraded, leading to the release of the VEGF aptamer, which acts as a potential inhibitor of the angiogenetic regeneration of blood vessels by VEGF. As calcination of the VEGF-generated blood vessels leads to blindness of diabetic patients, the functional NMOFs might act as "smart" materials for the treatment of macular diseases. The potential cytotoxicity of the NMOFs originated from the GOx-generated H 2 O 2 is resolved by the co-immobilization of the H 2 O 2 -scavanger catalase in the NMOFs.

Cu 2+ -functionalized carbon nitride nanoparticles (Cu 2+ -g-C 3 N 4 NPs), ∼200 nm, and Cu 2+ -carbon dots (Cu 2+ -C-dots), ∼8 nm, act as horseradish peroxidase-mimicking catalysts. The nanoparticles catalyze the generation of chemiluminescence in the presence of luminol/H 2 O 2 and catalyze the oxidation of dopamine by H 2 O 2 to form aminochrome. The Cu 2+ -g-C 3 N 4 -driven generation of chemiluminescence is used to develop a H 2 O 2 sensor and is implemented to develop a glucose detection platform and a sensor for probing glucose oxidase. Also, the Cu 2+ -C-dots are functionalized with the β-cyclodextrin (β-CD) receptor units. The concentration of dopamine, at the Cu 2+ -C-dots' surface, by means of the β-CD receptor sites, leads to a 4-fold enhancement in the oxidation of dopamine by H 2 O 2 to yield aminochrome compared to that of the unmodified C-dots.

Cu 2+ -ion-modified graphene oxide nanoparticles, Cu 2+ -GO NPs, act as a heterogeneous catalyst mimicking functions of horseradish peroxidase, HRP, and of NADH peroxidase. The Cu 2+ -GO NPs catalyze the oxidation of dopamine to aminochrome by H 2 O 2 and catalyze the generation of chemiluminescence in the presence of luminol and H 2 O 2 . The Cu 2+ -GO NPs provide an active material for the chemiluminescence detection of H 2 O 2 and allow the probing of the activity of H 2 O 2 -generating oxidases and the detection of their substrates. This is exemplified with detecting glucose by the aerobic oxidation of glucose by glucose oxidase and the Cu 2+ -GO NP-stimulated chemiluminescence intensity generated by the H 2 O 2 product. Similarly, the Cu 2+ -GO NPs catalyze the H 2 O 2 oxidation of NADH to the biologically active NAD + cofactor. This catalytic system allows its conjugation to biocatalytic transformations involving NAD + -dependent enzyme, as exemplified for the alcohol dehydrogenase-catalyzed oxidation of benzyl alcohol to benzoic acid through the Cu 2+ -GO NPs-catalyzed regeneration of NAD + .

A carbon nitride array (CNA) material has been constructed using a sacrificial diatom template. A regular carbon nitride nanorod array could be replicated from the periodic and regular nanochannel array of the template. The directional charge transport properties and high light harvesting capability of the CNA gives much better performance in splitting water to give hydrogen than its bulk counterpart. Furthermore, by combining with a rhodium complex as a mediator, the nicotinamide adenine dinucleotide (NADH) cofactor of many enzymes could be photocatalytically regenerated by the CNA. The rate of the in situ NADH regeneration is high enough to reverse the biological pathway of the three dehydrogenase enzymes, which then leads to the sustainable conversion of formaldehyde to methanol and also the reduction of carbon dioxide into methanol.