6 results on '"Castro-Claros, Juan Diego"'
Search Results
2. Shell-adductor muscle attachment and Ca2+ transport in the bivalves Ostrea stentina and Anomia ephippium
- Author
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Ministerio de Economía, Industria y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Castro-Claros, Juan Diego, Checa, Antonio G., Lucena, Cristina, Pearson, John R., Salas, Carmen, Ministerio de Economía, Industria y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Castro-Claros, Juan Diego, Checa, Antonio G., Lucena, Cristina, Pearson, John R., and Salas, Carmen
- Abstract
Among bivalve muscles, the adductors are particularly important for animal survival because they control valve closure. Most studies have addressed the type and morphology of this muscle in bivalves but few have focused on the mechanism that anchors it to the shell myostracum layer. Moreover, the possible calcium transport mechanism through the adductor muscle cells to the myostracum shell layer, which is necessary for bivalve biomineralisation, has never been addressed. Our results indicate that the muscle cell-shell attachment is mediated by the outer mantle epithelial cell layer, here termed tendon cells. These cells are modified at the muscle scar zone by the presence of actin cytoskeletal bundles, which anchor cells to the extracellular matrix via focal adhesion (or focal contact) junctions at the basal side and to extrapallial matrix at the apical side, both rich in collagen. From apical focal adhesions, bundles of collagen-rich fibres cross the extrapallial space and penetrate the myostracum shell layer. The latter constitutes one of the strongest anchoring structures among invertebrates. Numerous vesicles protrude from the tendon cells into the extrapallial space. TEM-EDX analysis reveals the presence of Ca inside some of these vesicles both in tendon cells and in the extrapallial space. This suggests a potential mechanism for calcium transport from cells to the myostracum. Statement of Significance: The interfaces between bivalve shells and muscular attachments are unique and of special interest as adhesive functional biomaterials, being one of the strongest invertebrate anchoring structures. We present an updated ultrastructural model of the adductor muscle-shell attachment. Muscle cells connect with the shell through epithelial `tendon cells`, which have a cytoskeleton of actin microfilaments that connect to the extracellular matrix via focal adhesions. Collagen-rich fibres arise from apical focal adhesions, cross the nanometric extrapallial space and pen
- Published
- 2021
3. Shell-adductor muscle attachment and Ca2+ transport in the bivalves Ostrea stentina and Anomia ephippium
- Author
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Castro-Claros, Juan Diego, primary, Checa, Antonio, additional, Lucena, Cristina, additional, Pearson, John R., additional, and Salas, Carmen, additional
- Published
- 2021
- Full Text
- View/download PDF
4. Shell-muscle attachment in the bivalves Ostrea stentina Payraudeau, 1826 and Anomia ephippium Linnaeus, 1758
- Author
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Castro-Claros, Juan Diego, Salas-Casanova, Maria del Carmen, Lucena-Serrano, Cristina, and Checa, Antonio
- Subjects
Bivalves ,Biomineralización ,Anatomía comparada ,Shell ,Muscle attachment ,Músculos ,Biología animal ,Bivalvos - Abstract
Comunicación a congreso The muscles of the mantle in bivalves attach to the shell valves and retract the mantle edges. Among them, the adductors are particularly important for the survival of the animal. Most studies have addressed the type and morphology of the muscular fibers and filaments, and a few have focused on the attachment of these muscles to the shell. However, the mechanism of transport through the muscle to the myostracum was never addressed. The goal of this research is to describe ultrastructure and the transport of material across the adductor muscle-myostracum attachment in Ostrea stentina and Anomia ephippium. The adductor muscles in both species are composed of “smooth” and “striated” muscles. The outer epithelium of the mantle continues across the adductor muscle area and their cells contain many vesicles. Bundles of filaments connect the muscular cells with the extrapallial space through hemidesmosomes. The extrapallial space (ca.100-150 nm thick) is filled with organic secretions from the vesicles of the mantle cells. Additional bundles of microfilaments of collagen fibers cross the extrapallial sheet, from the hemidesmosomes to inside the myostracum, where they form a network. EDX analysis shows the presence of calcium inside vesicles from the mantle cells, the extrapallial sheet and the myostracum network. The presence of a layer of cells between the muscular cells and the shell was confirmed the existence of this cell layer, which is the continuation of the outer mantle epithelium across the adductor muscle. The extrapallial space is replenished with secretions of the mantle cells and collagen fibers, seemingly originated in the hemidesmosomes. The presence of calcium inside some vesicles suggests that part of the calcium is transported thereby. It would be interesting to study whether calcium is transported as amorphous calcium carbonate or as a crystalline phase. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
- Published
- 2019
5. Reproduction of the anthozoan Anemonia sulcata (Pennant, 1777) in southern Spain: from asexual reproduction to putative maternal care
- Author
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Utrilla, O. (Olga), Castro-Claros, Juan Diego, Urra, J. (Javier), Navas, Francisco David, and Salas, C. (Carmen)
- Abstract
Anemonia sulcata (Pennant, 1777) is a common shallow water cnidarian from rocky platform and boulder beaches in southern Spain, where it is a popular seafood item with an increasing fshery. To aid in the management of a sustainable fshery, a study on the reproduction of A. sulcata in the littoral of Malaga (southern Spain) was performed from November 2014 to September 2015, using histological methods. A total of 123 specimens were examined, with a size range (as diameter of the pedal disc) from 1.1 to 48.2 mm. The sex ratio was signifcantly biased to females, with 1.7 females: 1 male (χ2=4.45, p
- Published
- 2019
6. Shell-adductor muscle attachment and Ca 2+ transport in the bivalves Ostrea stentina and Anomia ephippium.
- Author
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Castro-Claros JD, Checa A, Lucena C, Pearson JR, and Salas C
- Subjects
- Animal Shells, Animals, Anomia, Biomineralization, Bivalvia, Ostrea
- Abstract
Among bivalve muscles, the adductors are particularly important for animal survival because they control valve closure. Most studies have addressed the type and morphology of this muscle in bivalves but few have focused on the mechanism that anchors it to the shell myostracum layer. Moreover, the possible calcium transport mechanism through the adductor muscle cells to the myostracum shell layer, which is necessary for bivalve biomineralisation, has never been addressed. Our results indicate that the muscle cell-shell attachment is mediated by the outer mantle epithelial cell layer, here termed tendon cells. These cells are modified at the muscle scar zone by the presence of actin cytoskeletal bundles, which anchor cells to the extracellular matrix via focal adhesion (or focal contact) junctions at the basal side and to extrapallial matrix at the apical side, both rich in collagen. From apical focal adhesions, bundles of collagen-rich fibres cross the extrapallial space and penetrate the myostracum shell layer. The latter constitutes one of the strongest anchoring structures among invertebrates. Numerous vesicles protrude from the tendon cells into the extrapallial space. TEM-EDX analysis reveals the presence of Ca
2+ inside some of these vesicles both in tendon cells and in the extrapallial space. This suggests a potential mechanism for calcium transport from cells to the myostracum. STATEMENT OF SIGNIFICANCE: The interfaces between bivalve shells and muscular attachments are unique and of special interest as adhesive functional biomaterials, being one of the strongest invertebrate anchoring structures. We present an updated ultrastructural model of the adductor muscle-shell attachment. Muscle cells connect with the shell through epithelial `tendon cells`, which have a cytoskeleton of actin microfilaments that connect to the extracellular matrix via focal adhesions. Collagen-rich fibres arise from apical focal adhesions, cross the nanometric extrapallial space and penetrate the myostracum where they form an organic network. Calcium is present inside vesicles that are released into the extrapallial space. The lack of direct cellular control on secretion restricts the myostracal microstructure to prismatic aragonitic similar to its inorganic counterpart., Competing Interests: Declaration of Competing Interest The authors declare having no conflict of interest regarding this publication., (Copyright © 2020 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)- Published
- 2021
- Full Text
- View/download PDF
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