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4. The developmental basis of allometry

Author

Castilho, L. V.

Subjects
591.35
Abstract

This thesis investigates the role played by molecular signalling pathways in organ size determination in Drosophila melanogaster. First, the analysis of wing mosaics generated by mitotic recombination showed that both the insulin receptor InR and its downstream target chico have a strong and largely local autonomous reducing effect on wing size. Position of the mutant tissue in the mosaic wings determines the intensity of size deficits, since the posterior wing compartment is more severely affected than the anterior. A second insulin receptor was analysed and shown to perform no role in size determination. Additionally, the TGF-b/activin surface receptor babo causes much weaker size reductions, even though cells deficient for the gene have extreme difficulty to proliferate. This corroborates the idea that genes with an important role in cell proliferation do not necessary play a major role in determining final organ size. Finally, the small size phenotype of vein mutants (a member of the EGF pathway) was reassessed, and the results did not corroborate previous claims that the reduced size of vein mosaic wings is caused by an overall reduction of the wing. On the contrary (and consistently with the action of insulin and TGF-beta pathway genes), vein seems not to interfere with the size of adjacent non-vein regions of the mosaics. This adds a question mark to Garcia-Bellido's Entelechia model of wing development. A second issue addressed in this project is the known interaction between size and environmental factors. It is widely believed that the insulin pathway is responsible for converting nutrient availability into growth. Such hypothesis implies that the size of insulin pathway mutants should respond less than wildtype flies (or not respond at all) to starvation. Experiments showed that in fact chico and InR mutants do not respond to starvation, contrary to the intense size decrease observed in malnourished wildtype flies.

Published
2002

5. MODELAGEM DE UMA MÁQUINA DE RANHURA DE TUBO METÁLICO AUTOMATIZADOS

Author

RODRIGUES, F., SOUSA, D., CASTILHO, L., RIBEIRO, W., and NORIEGA, C.L.

Subjects
Palavras-chave: Máquina de Grooved, Automação Industrial, Ranhuras, Tubos Metálicos, Otimização do processo
Abstract

A automação industrial surge como uma das grandes apostas para os próximos anos, especialmente em razão do alto desenvolvimento tecnológico. Com isso, os equipamentos já conhecidos tendem a passar por uma reestruturação, buscando melhores condições e otimização do processo como um todo. O presente trabalho possui como objetivo a automação de uma máquina de Grooved para automação de ranhuras em tubos metálicos. Para que possa atingir esse objetivo, os seguintes objetivos específicos foram definidos: Identificar os parâmetros e caracterizar o que compõe a máquina de Grooved; Detalhar a importância da automação industrial para o setor da mecânica; analisar o processo realizado para automação da máquina, com suas principais vantagens e a esteira de construção do modelo., AUTOMATED METAL TUBE SLOTTING MACHINE MODELING

Published
2023
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14. Vox Sanguinis International Forum on application of fetal blood grouping: summary

Author

Daniels, G., Finning, K., Lozano, M., Hyland, C. A., Liew, Y.‐W., Powley, T., Castilho, L., Bonet Bub, C., Kutner, J. M., Banch Clausen, F., Christiansen, M., Sulin, K., Haimila, K., Legler, T. J., Lambert, M., Ryan, H., Ní Loingsigh, S., Matteocci, A., Pierelli, L., Dovc Drnovsek, T., Bricl, I., Nogués, N., Muñiz‐Diaz, E., Olsson, M. L., Wikman, A., de Haas, M., van der Schoot, C. E., Massey, E., and Westhoff, C. M.

Published
2018
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23. Transfusion service management of sickle-cell disease patients

Author

Yazer, M. H., Lozano, M., Crighton, G., Greenway, A., Comande, M., Savoia, H., Wood, E., Gilli, S., Castilho, L., Saad, Sara T.O., Galactéros, F., Noizat-Pirenne, F., Pazgal, I., Stark, P., Orlin, Y., Perseghin, P., Masera, N., Cela, E., Anguita, J., Wikman, A., and Delaney, M.

Published
2016
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27. RHCEvariant alleles and risk of alloimmunization in Brazilians

Author

Arnoni, C.P., Vendrame, T.A.P., Silva, F.S., Cortez, A.J.P., Latini, F.R.M., and Castilho, L.

Abstract

Variant RHCEalleles are found mainly in Afro-descendant individuals, as well as in patients with sickle cell disease (SCD). The most common variants are related to the RHCE*ceallele, which can generate partial e and c antigens. Although RHCEvariant alleles have been extensively studied, defining their clinical significance is a difficult task. We evaluated the risk of RhCE alloimmunization as a consequence of partial antigens in patients with a positive phenotype transfused with red blood cell (RBC) units with the corresponding antigen. A retrospective study was performed with Brazilian patients, evaluating the number of antigen-positive transfused RBC units (incompatible due to partial antigen) in 27 patients with SCD carrying RHCEvariant alleles who did not develop antibodies as well as evaluating the variants present in 12 patients with partial phenotype and correlated antibody (one patient with SCD and 11 patients with other pathologies). Two patients showed variant alleles with molecular changes that had not yet been described. Variant RHCEalleles were identified in a previous study using molecular methods. RHCE*ceVS.01was the most frequent allele found among the patients without antibodies. Six patients with partial c antigen had a mean of 3.8 c+ RBC units transfused, and 10 patients with partial e antigen were exposed for a mean of 7.2 e+ RBC units. Among the variant alleles found in alloimmunized patients, the most frequent was RHCE*ceAR,which was found in five patients; the antibodies developed were anti-hrSand/or anti-c. Our results showed that RHCE*ceVS.01is indeed the most frequent variant allele in our cohort of patients with SCD, but the partial antigens that were identified have low risk of alloimmunization. RHCE*ceARis the most impactful variant in the Brazilian population with high risk of alloimmunization and clinically significant anti-hrSformation.

Published
2022
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