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24 results on '"Castellini, Horacio"'

1. Study of non-linear viscoelastic behavior of the human red blood cell

Author

Castellini, Horacio and Riquelme, Bibiana

Subjects
Quantitative Biology - Cell Behavior, Condensed Matter - Soft Condensed Matter, Physics - Biological Physics
Abstract

The non-linear behavior of human erythrocytes subjected to shear stress was analyzed using data series from the Erythrocyte Rheometer and a theoretical model was developed. Linear behavior was eliminated by means of a slot filter and a sixth order Savisky-Golay filter was applied to the resulting time series that allows the elimination of any possible white noise in the data. A fast Fourier transform was performed on the processed data, which resulted in a series of frequency dominant peaks. Results suggest the presence of a non-linear quadratic term in the Kelvin-Voigt phenomenological model. The correlation dimension studied through recurrence quantification analysis gave C2=2.58. Results suggest that the underlying dynamics is the same in each RBC sample corresponding to healthy donors.

Published
2018

3. On the propagation of social epidemics in social networks under S.I.R. model

Author

Castellini, Horacio and Romanelli, Lilia

Subjects
Nonlinear Sciences - Adaptation and Self-Organizing Systems
Abstract

The S.I.R. model (Susceptible, Infected, Recovered or Died) was proposed by chemistry Willam Kermack (1927) and the mathematician G. Mc. Kendrick (1932). the model supposes to divide to the individuals of a population in three categories. Susceptible to be infected, Infected and Recovered (immune or died by the disease). On the other hand has been a similarity in the evolution of epidemics of infect aerial, the computer science propagation of virus and the propagation of social paradigms (fashion, rumor, etc.) it calls modernly ``Social Epidemics''. In this work it is tried to use this model in different types from social networks, real or not. In order to evaluate a meta-analysis of results allows to investigate under wich conditions the topology of the network is excellent and that networks are equivalent. The result obtained can have relevance in study of propagation of epidemics of different types., Comment: 10 pages

Published
2007

4. On study of nonlinear viscoelastic behavior of red blood cell membrane

Author

Castellini, Horacio, Riquelme, Bibiana, and Foresto, Patricia

Subjects
Nonlinear Sciences - Chaotic Dynamics
Abstract

The linear viscoelastic behavior of the red blood cell membrane of mammal and human was studied in previous works proposing different experimental methods to determine their viscoelastic parameters. In the present work the nonlinear component of dynamic viscosity of the red blood cell membrane by nonlinear time series analysis is used. For such aim, it obtained time series of test in vitro of samples of humans and rats red blood cells using the Erythrodeformeter in oscillating regime. The signal filtrate suppresses any linear behavior as well as represented by a system of linear ordinary differential equations. The test shown as much in humans as in rats resonance frequencies associated to an attractor of unknown nature independently of excitation in the physiological range. The preliminary studies shown that attractor could be correspond to a complex form bull. These results allow to extend the present knowledge on dynamic of the cellular membrane to similar stimulus which happens in the blood circulation and it will allows to make better models of the same one., Comment: 12 pages and 3 figures

Published
2007

5. Social network from communities of electronic mail

Author

Castellini, Horacio and Romanelli, Lilia

Subjects
Nonlinear Sciences - Chaotic Dynamics, Nonlinear Sciences - Adaptation and Self-Organizing Systems, Physics - Physics and Society
Abstract

Social networks are analyzed as graphs under the scope of discrete mathematics which have a great range of applications in different contexts such as: technology, social phenomena and biological systems. At the present this theory gives a set of tools for a phenomenological analysis that would be difficult or almost impossible with a different approach. In this work social networks for different technical communities from electronic mail and ``News'' in Spanish language are constructed. The algorithm was based on the use of RFC2822 standards and RFC1036 to arm threads of messages. The results are quite different from that obtained by another kind of community as the jazz musicians community. Nevertheless they show an analogy to random graphs obtained by the ``Configuration Model'' method. This points the attention that some generalization assumptions are not correct., Comment: 11 pages and 4 figures

Published
2005

7. Preliminar study of the effects of gamma radiation on human redblood cells

Author

Estrada, Ezequiel, Castellini, Horacio V., Acosta, Andrea, Di Tullio, Liliana, Borraz, Javier, Chinellato, Alejandro, Tack, Ivan, D’Arrigo, Mabel, Riquelme, Bibiana Doris, and Galassi, Mariel Elisa

Subjects
Erythrocyte, RADIACIÓN GAMMA, VISCOELASTICIDAD, purl.org/becyt/ford/1 [https], Gamma Radiation, ERITROCITO, purl.org/becyt/ford/3.5 [https], Viscoelasticity, purl.org/becyt/ford/3 [https], purl.org/becyt/ford/1.3 [https], HEMORREOLOGÍA, purl.org/becyt/ford/1.6 [https]
Abstract

En este estudio se analizaron los parámetros viscoelásticos y de agregación en glóbulos rojos humanos sometidos a los procedimientos habituales de irradiación gamma con fines transfusionales. Las muestras fueron irradiadas a diferentes dosis a fin de determinar los posibles cambios hemorreológicos que pudieran afectar a la salud de los pacientes y su relación con las modificaciones bioquímicas observadas. Los resultados obtenidos muestran alteraciones en el tiempo de agregación, en la viscosidad superficial de membrana y en el tamaño de los agregados eritrocitarios en las muestras irradiadas, sugiriendo que el daño producido por la radiación ionizante afecta a las propiedades físicas de la membrana del glóbulo rojo en diferentes niveles In this study, the alterations in viscoelastic and aggregation parameters of red blood cells were analyzed for usual gamma irradiation procedures for transfusion purposes. In order to determine possible hemorheological changes that may affect the health of patients and their relationship with the biochemical changes observed, the blood samples were irradiated at different doses. The results show alterations in the erythrocyte aggregation time, in the membrane surface viscosity and in the size of the aggregates in the irradiated samples, suggesting that the damage produced by the ionizing radiation affects the physical properties of red blood cell membrane at different levels. Fil: Estrada, E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Castellini, H.. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina Fil: Acosta, A.. Centro Regional de Hemoterapia; Argentina Fil: Di Tullio, L.. Centro Regional de Hemoterapia de Rosario; Argentina Fil: Borraz, J.. Centro Regional de Hemoterapia de Rosario; Argentina Fil: Chinellato, A.. Centro Regional de Hemoterapia de Rosario; Argentina Fil: Tack, I.. Centro Regional de Hemoterapia de Rosario; Argentina Fil: D'Arrigo, M.. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina Fil: Riquelme, Bibiana Doris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Galassi, Mariel Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina

Published
2020

12. In vitro alterations of erythrocyte aggregation by action of Trichinella spiralis newborn larvae

Author

Ponce de León Patricia, Riquelme Bibiana, Toderi Martín, and Castellini Horacio

Subjects
Erythrocyte Aggregation, 0301 basic medicine, CIENCIAS MÉDICAS Y DE LA SALUD, Physiology, Ciencias Físicas, 030231 tropical medicine, Cell, Trichinella spiralis, Ciencias de la Salud, Medicina Clínica, Trichinosis, TRICHINELLOSIS, Erythrocyte aggregation, Incubation period, IMAGE DIGITAL ANALYSIS, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Physiology (medical), medicine, Animals, Larva, Salud Ocupacional, biology, Trichinellosis, Hematology, biology.organism_classification, medicine.disease, Molecular biology, In vitro, Sialic acid, Astronomía, 030104 developmental biology, medicine.anatomical_structure, ERYTHROCYTE AGGREGATION, OPTICAL CHIP, chemistry, NEWBORN LARVAE, Female, Medicina Critica y de Emergencia, Cardiology and Cardiovascular Medicine, CIENCIAS NATURALES Y EXACTAS
Abstract

Possible changes in the erythrocyte membrane, by in vitro interaction with newborn larvae of T. spiralis (NL), were evaluated analyzing the alterations in erythrocyte aggregation by digital image analysis and laser transmission in a new optical chip aggregometer. NL were obtained from CBi mice infected with T. spiralis. RBCs samples from healthy donors where in vitro exposed to NL (concentration (3000±500) larvae/mL) to assess its effect on RBC aggregation. Individual cell Coefficient (CCA) and aggregation parameter (S) were calculated by digitally processingRBCaggregate images, indicating the amount and size of the erythrocyte aggregates present. Also, size distribution of aggregate was analyzed. Kinetic aggregation parameters (Amp750 and t1/2) were calculated with a new optical chip aggregometer. Results show significant alterations in erythrocyte aggregability due the in vitro action of T. spiralis larvae increasing incubation time. These results are possibly related to the loss of surface sialic acid as it is captured by NL. Obtained results suggest thatNLcould produce hemorheological alterations in the host, which could be related to thrombosis and anemia reported in some patients with trichinosis. Fil: Ponce De Leon, Patricia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Toderi Cicchini, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina Fil: Castellini, Horacio Virginio. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina Fil: Riquelme, Bibiana Doris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina

Published
2016

15. Application of a digital image procedure to evaluate microstructure of caseinate and soy protein acid gels

Author

Ingrassia, Romina, Costa, Juan Pablo, Hidalgo, María Eugenia, Mancilla Canales, Manuel Arturo, Castellini, Horacio V., Riquelme, Bibiana Doris, Risso, Patricia Hilda, and Brandelli, Adriano

Subjects
Image Textural Analysis, Protein Acid Gels, Conventional Optical Microscopy, Microstructure, Rheological Properties
Abstract

Acid gelation of proteins is commonly used in the food industry and it can be induced by the addition of glucono-delta-lactone (GDL). The aim of this work was to use textural analysis of images in order to assess possible changes in the microstructure of bovine sodium caseinate (NaCAS) and soy protein isolate (SPI) acid gels. The gelation rate of NaCAS related to the amount of GDL was evaluated. Also, the effect of the presence of NaCAS hydrolyzates obtained at different hydrolysis times by the enzyme of Bacillus sp. P7 was studied. Finally, SPI acid gels were evaluated in the presence of whey soy protein isolate (WSP) in different ratios. The gel images were obtained by conventional optical microscopy and texture parameters were obtained by using specific programs which were developed in Python language. Shannon entropy, smoothness, mean normalized grey level variance and uniformity were analyzed as estimators of the texture of the images obtained. Results obtained in the evaluated systems showed that these parameters were able to represent the structural changes in the gel network, as changes in size of pores or in degree of compactness. Also, these results were contrasted with rheological properties of the systems evaluated. Fil: Ingrassia, Romina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina. Fil: Costa, Juan Pablo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina. Fil: Hidalgo, María. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina. Fil: Mancilla Canales, Manuel Arturo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina. Fil: Castellini, Horacio V. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina. Fil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina. Fil: Risso, Patricia Hilda. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina. Fil: Risso, Patricia Hilda. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina.

Published
2013

16. Análisis digital de imágenes de fase para el estudio de cultivos de células madre mesenquimales

Author

Castellini, Horacio Virginio, Dumas, Dominique, Hupont, Sébastien, Fontana, Alicia Beatriz Albina, Delannoy, Marcela María, Oliveros, Miguel, Magdalou, J., and Riquelme, B.

Subjects
NON-INVASIVE TECHNIQUES, DIFERENCIACION CELULAR, PHASE IMAGE, CELL CULTURE, TECNICAS NO INVASIVAS, CELULAS MADRES MESENQUIMATICAS, CELL DIFFERENTIATION, MESENCHYMAL STEM CELL, IMAGEN DE FASE, CULTIVO CELULAR
Abstract

En los últimos años se ha visto cómo las células madre han pasado de ser un concepto de interés científico principalmente en el campo de la biología, a ocupar tantas páginas en las revistas científicas como en la prensa. Los conocimientos que en este campo de la medicina se vienen produciendo de forma diaria ha disparado las expectativas de los enfermos y de los médicos de que las células madre vayan a contribuir a la curación de múltiples enfermedades humanas devastadoras como la diabetes, la enfermedad de Parkinson, el infarto de miocardio u otras. Se presenta una técnica óptica no invasiva, basada en el análisis digital de imágenes de fase que permite analizar el cultivo de células madre mesenquimales con el objetivo de determinar los parámetros de imagen representativos del crecimiento y de la diferenciación de estas células. Estos experimentos contribuirán a un mejor entendimiento de la dinámica de la células madres, ayudando al avance de diversas aplicaciones médicas In recent years we have seen how stem cells or stem has grown from a concept of scientific interest mainly in the field of biology, to occupy many pages in scientific magazines and the press. The knowledge in this field of medicine have been occuring almost daily so triggered the expectations of patients and physicians that stem cells will helpcure many devastating human diseases such as diabetes, the disease Parkinson, myocardial infarction or many other. We present a non-invasive optical technique bsaed on digital image analysis phase allowing to observe the culture og mesenchymal stem cells. This make is possible to analyze an quantify the dynamic changes during growth and cell differentiation. We analyze different stages of the evolution of mesenchymal stem cell in order to determine the imaging parameters representative of growth and differentiation of these cells. The expermients contribute to a better understanding of the dynamics of stem cells, helping to advance medical application Fil: Castellini, Horacio Virginio. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura (UNR-FCEIA). Santa Fe. Argentina Fil: Dumas, Dominique. Université de Lorraine. Plateforme d’Imagerie et de Biophysique Cellulaire (CNRS-PTIBC-IBISA). Nancy. Francia Fil: Hupont, Sébastien. Université de Lorraine. Plateforme d’Imagerie et de Biophysique Cellulaire (CNRS-PTIBC-IBISA). Nancy. Francia Fil: Fontana, Alicia Beatriz Albina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR-FBIOyF). Santa Fe. Argentina Fil: Delannoy, Marcela María. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR-FBIOyF). Santa Fe. Argentina Fil: Oliveros, Miguel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR-FBIOyF). Santa Fe. Argentina Fil: Magdalou, J.. Université de Lorraine. Plateforme d’Imagerie et de Biophysique Cellulaire (CNRS-PTIBC-IBISA). Nancy. Francia Fil: Riquelme, B.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR-FBIOyF). Santa Fe. Argentina

Published
2011

19. Efecto de los copolímeros Semo B86 y Semo B124 sobre la viscoelasticidad dinámica y la capacidad de agregación de eritrocitos humanos

Author

Danieli, Valentina, Fontana, Alicia Beatriz Albina, Alessi, Adriana, Castellini, Horacio Virginio, Delannoy, Marcela María, Foresto, Patricia, Grandfils, C., Valverde, J., and Riquelme, B.

Subjects
VISCOELASTICIDAD, IMAGE PROCESSING, PROCESAMIENTO DE IMAGENES, ERYTHROCYTE AGGREGATION, BIOCOMPATIBILIDAD, AGREGACION ERITROCITARIA, VISCOELASTICITY, POLYETHYLENE GLYCOL, POLIETILENGLICOL, POLICATIONES, POLYCATIONS, BIOCOMPATIBILITY
Abstract

Se analizaron las posibles alteraciones que los copolímeros sintéticos policatión-PEG Semo B86 y Semo B124 producen en la viscoelasticidad dinámica de los eritrocitos humanos con un Eritrodefórmetro y en su morfología y propiedades de agregación utilizando microscopía convencional y confocal. Además, se estudió la influencia del tiempo de incubación de las células con estos compuestos a fin de obtener el más adecuado para futuras experiencias. Los resultados mostraron que los parámetros viscoélasticos dinámicos analizados se mantuvieron dentro del rango de valores normales al aumentar el tiempo de incubación, para ambos copolímeros. Por otra parte se observó que los eritrocitos tratados con ambos copolímeros muestran alteraciones en la distribución del tamaño de sus agregados. Estos resultados ayudan a comprender los mecanismos de interacción de estos copolímeros con la membrana eritrocitaria, brindando herramientas útiles para asegurar su biocompatibilidad con los componentes sanguíneos y su optimización para fines terapéuticos, ampliándose además el conocimiento actual sobre la dinámica de la membrana celular. The potential disturbances in dynamic viscoelastic properties of human erythrocytes produced by the synthetic copolymers polycation-PEG Semo B86 y Semo B124 were analized using an Eritrodeformeter, as well as erythrocyte aggregation properties and morphology through conventional and confocal microscopy. Moreover, the influence of incubation time of cells with these compounds was studied so as to obtain the most suitable one for future experiences. The dynamic viscoelastic parameters analized for samples treated with either copolymers did not show any significant alterations compared to control sample, for all incubation times studied. On the other hand, modificarions in the size distribution of erythrocyte aggregates for treated samples were observed. These results will help to understand the interaction mechanisms of these copolymers with the erythrocyte membrane, providing useful tools to ensure their biocompatibility with blood components and to optimize them for therapeutic purposes, as well as extending the current knowledge about cellular membrane dynamics Fil: Danieli, Valentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR-FBIOyF). Santa Fe. Argentina Fil: Fontana, Alicia Beatriz Albina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR-FBIOyF). Santa Fe. Argentina Fil: Alessi, Adriana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR-FBIOyF). Santa Fe. Argentina Fil: Castellini, Horacio Virginio. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura (UNR-FCEIA). Santa Fe. Argentina Fil: Delannoy, Marcela María. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR-FBIOyF). Santa Fe. Argentina Fil: Foresto, Patricia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR-FBIOyF). Santa Fe. Argentina Fil: Grandfils, C.. Universidad de Lieja. Centre Interfacultaire des Biomatériaux (CEIB). Lieja. Bélgica Fil: Valverde, J.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR-FBIOyF). Santa Fe. Argentina Fil: Riquelme, B.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR-FBIOyF). Santa Fe. Argentina

Published
2007

24. ESTUDIO DE LA ESTRUCTURA DE QUESOS FUNCIONALES CON EL ADICIONADO DE SEMILLAS DE CHÍA MEDIANTE EL ANÁLISIS DIGITAL DE IMÁGENES.

Author

Bortolotto, Natalia, Rozycki, Sergio, Castellini, Horacio, and Riquelme, Bibiana

Abstract

In recent years, in the cheese industry the applications have focused on the study of the relationship between microstructure, texture and sensory properties that constitute a parameter of interest for optimizing manufacturing practices. The challenge is how to reduce or remove cholesterol from these products, without completely eliminating fat, which provides vitamin A and D, essential fatty acids and textural properties. For this reason, in this work the microstructure of spreadable cheese reduced in fat type and cholesterol, with added chia seed meal, using the digital image analysis was assessed. Comparing the microstructure as a function of varying fat content but equal percentage of chia flour, it was concluded that the cheeses with higher fat content (10 % fat) showed free cavities larger and more heterogeneous matrix that cheeses with lower fat content (6 % fat). When comparing the texture parameters of the images it was observed, for the same content of chia, the grey scale entropy increased and the uniformity decreased with fat content, which may be related with a greater variability in the size of the cavities. This is related to the data obtained previously by texture profile analysis, where it was observed that while it was increased the fat % it was also increasing the hardness parameter and the consistency value. [ABSTRACT FROM AUTHOR]

Published
2015

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