29 results on '"Cassan D"'
Search Results
2. Blending chitosan-g-poly(caprolactone) with poly(caprolactone) by electrospinning to produce functional fiber mats for tissue engineering applications
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De Cassan, D., Becker, A., Glasmacher, B., Roger, Y., Hoffmann, A., Gengenbach, T.R., Easton, C.D., Hänsch, R., and Menzel, H.
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Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie ,X-ray photoelectron spectroscopy ,Surface enrichment ,X ray photoelectron spectroscopy ,Cellular attachments ,Blend ,Biological performance ,Cell compatibility ,Poly (epsiloncaprolactone) ,Biomechanics ,Confocal laser scanning microscopy ,Tissue engineering ,Crystallinity ,electrospinning ,Chitosan ,Photons ,Tissue ,Tissue engineering applications ,polycaprolacton ,Blending ,Fibers ,ddc:540 ,Polymeric implants ,Scanning electron microscopy ,Photoelectrons - Abstract
Use of electrospun fiber mats for tissue engineering applications has become increasingly prominent. One of the most important polymers in research, poly(ε-caprolactone) (PCL), however, lacks biological performance, easy access to modifications and cellular recognition sites. To improve these properties and to enable further modifications, PCL was blended with chitosan grafted with PCL (CS-g-PCL) and subsequently processed via electrospinning. In this way, chitosan was enriched at the fiber's surface presenting cationic amino groups. The fiber mats were analyzed by various techniques such as scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and X-ray photoelectron spectroscopy (XPS). Furthermore, analyzing thermal properties and crystallinity, showed that an increased content of CS-g-PCL in blend composition leads to a higher overall crystallinity in produced fiber mats. Blending CS-g-PCL into PCL significantly increased initial cellular attachment and proliferation as well as cell vitality, while maintaining adequate mechanical properties, fiber diameter, and interstitial volume. As proof of principle for easy access to further modification, fluorescently labeled alginate (Alg-FA) was attached to the fiber's surface and verified by CLSM. Hence, blending CS-g-PCL with PCL can overcome an inherent weakness of PCL and create bioactive implants for tissue engineering applications. © 2019 The Authors. Journal of Applied Polymer Science published by Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 137, 48650. © 2019 The Authors. Journal of Applied Polymer Science published by Wiley Periodicals, Inc.
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- 2019
3. Étude de l’échelle d’estime de soi de Rosenberg dans un échantillon de lycéens
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Chabrol, H., Carlin, E., Michaud, C., Rey, A., Cassan, D., Juillot, M., Rousseau, A., and Callahan, S.
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- 2004
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4. SALIVARY IGA AND RESPIRATORY ILLNESS IN TRIATHLETES DURING AN ALTITUDE TRAINING CAMP IN COLORADO SPRINGS
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Mercier, B, Millet, G P., Saez, A, Rouot, S, Castex, F, Libicz, S, Cassan, D, and Varray, A
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- 2003
5. Policing Opportunities and Threat
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van den Born, A, Allen, K, Andrei, D, Barlage, M, Bayerl, Saskia, Betteridge, K, Betz, M, Bisogni, F, Birdi, K, Casey, R, Cassan, D, Christe-Zeyse, J, Costanzo, P, Denef, S, Gasco, M, Graham, L, Gruschinske, M, Horton, Kate, Jacobs, Gabriele, Jochoms, T, Mouhanna, C, Otoiu, C, Pautz, H, Rogiest, S, Sapulete, S, Stojanovski, T, Vallet, N, van den Oord, A, van Witteloostuijn, A, Vit, M, and Department of Organisation and Personnel Management
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- 2011
6. ICT Trends in European Policing
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Denef, S, Kaptein, N, Bayerl, Saskia, Birdi, K, Bisogni, F, Cassan, D, Christe-Zeyse, J, Costanzo, P, Gasco, M, Horton, Kate, Jacobs, Gabriele, Jochoms, T, Krstevska, K, Mirceva, S, van den Oord, A, Otoiu, C, Rajkovchevski, R, Reguli, Z, Stojanovski, T, Vonas, G, and Department of Organisation and Personnel Management
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- 2011
7. Comparison between strip and rib SOI microwaveguides for intra-chip light distribution
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Vivien F. Grillot E. Cassan D. Pascal S. Lardenois A. Lupu S. Laval, L., Heitzmann And J.-M. Fédéli, M., Institut d'électronique fondamentale (IEF), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
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- 2005
8. ICT trends in European policing (Deliverable 4.1)
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Denef, S. (Sebastian), Kaptein, N. (Nico), Bayerl, P.S. (Saskia), Birdi, K. (Kamal), Bisogni, F. (Fabio), Cassan, D. (Damien), Christe-Zeyse, J. (Jochen), Costanzo, P. (Pietro), Gasco, M. (Mila), Horton, K.E. (Kate), Belschak-Jacobs, G. (Gabriele), Jochoms, T. (Theo), Krstevska, K. (Katerina), Mirceva, S. (Stojanka), Oord, A. (Ad) van den, Otoiu, C. (Catalina), Rajkovchevski, R. (Rade), Reguli, Z. (Zdenko), Stojanovski, T. (Trpe), Vonas, G. (Gabriel), Denef, S. (Sebastian), Kaptein, N. (Nico), Bayerl, P.S. (Saskia), Birdi, K. (Kamal), Bisogni, F. (Fabio), Cassan, D. (Damien), Christe-Zeyse, J. (Jochen), Costanzo, P. (Pietro), Gasco, M. (Mila), Horton, K.E. (Kate), Belschak-Jacobs, G. (Gabriele), Jochoms, T. (Theo), Krstevska, K. (Katerina), Mirceva, S. (Stojanka), Oord, A. (Ad) van den, Otoiu, C. (Catalina), Rajkovchevski, R. (Rade), Reguli, Z. (Zdenko), Stojanovski, T. (Trpe), and Vonas, G. (Gabriel)
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In this report we present the results from interviews and document analyses of current and planned information and communication technology (ICT) projects with police forces from 10 European countries and from interviews with technology vendors in the f
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- 2011
9. 639 Recovery of Left Ventricular Systolic Function in New Onset Heart Failure
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Chiu, M., primary, Haddad, H., additional, Davies, R.A., additional, Cassan, D., additional, Begin, S., additional, Coulas, S., additional, and Mielniczuk, L.M., additional
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- 2012
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10. P036 - Part respective de glucides et de lipides oxydés à différents niveaux d’exercice chez sédentaires et sportifs : mise en évidence d’un profil physiologique de « glucodépendance précoce »
- Author
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Varlet-Marie, E., primary, Cassan, D., additional, Fédou, C., additional, Mercier, J., additional, and Brun, J.-F., additional
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- 2005
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11. An 8.4mW/Gb/s 4-lane 48Gb/s multi-standard-compliant transceiver in 40nm digital CMOS technology.
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Ramezani, M., Abdalla, M., Shoval, A., Van Ierssel, M., Rezayee, A., McLaren, A., Holdenried, C., Pham, J., So, E., Cassan, D., and Sadr, S.
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- 2011
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12. Early surgery for failure after chemoradiation in operable thoracic oesophageal cancer. Analysis of the non-randomised patients in FFCD 9102 phase III trial: Chemoradiation followed by surgery versus chemoradiation alone
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Julie, Vincent, Christophe, Mariette, Denis, Pezet, Emmanuel, Huet, Franck, Bonnetain, Olivier, Bouché, Thierry, Conroy, Bernard, Roullet, Jean-François, Seitz, Jean-Philippe, Herr, Frédéric, Di Fiore, Jean-Louis, Jouve, Laurent, Bedenne, M, Ducreux, CHU Dijon, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Hôpital Claude Huriez [Lille], CHU Lille, CHU Clermont-Ferrand, Nutrition, inflammation et dysfonctionnement de l'axe intestin-cerveau (ADEN), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Normandie Université (NU), Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de chirurgie digestive [CHU Rouen], Normandie Université (NU)-Normandie Université (NU)-CHU Rouen, Fédération Francophone de la Cancérologie Digestive, FFCD, Hôpital Robert Debré, Hôpital Robert Debré-Centre Hospitalier Universitaire de Reims (CHU Reims), Maladies chroniques, santé perçue, et processus d'adaptation (APEMAC), Université Paris Descartes - Paris 5 (UPD5)-Université de Lorraine (UL), Institut de Cancérologie de Lorraine - Alexis Vautrin [Nancy] (UNICANCER/ICL), UNICANCER, Hôpital de la Milétrie, Centre hospitalier universitaire de Poitiers (CHU Poitiers), Hôpital de la Timone [CHU - APHM] (TIMONE), Hôpital privé Sainte-Marie - Ramsay Générale de Santé, Hôpital Charles Nicolle [Rouen], Butel J, Desselle P, Brice JC, Tissot B, Votte-Lambert A, Joly J, Burtin P, Arnaud JP, Cellier P, Estermann F, Chauvet B, Maringe E, Ozanne F, Varlet F, Becouarn Y, Avril A, Rougier P, Nordlinger B, Vincendet M, Charneau J, Pillon D, Stremsdoerfer N, Pelletier M, Clavero-Fabri MC, Leduc B, Segol P, Argouach LP, Roussel A, Maurel J, Salame R, Lacourt J, Janoray P, Ruget O, Baudet-Klepping D, Dupont G, Bommelaer G, Ruszniewski P, Hammel P, Chaussade S, Dousset B, Denis B, Wagner JD, Tamby E, Petit T, Weiss AM, Barbare JC, Jouve JL, Phelip JM, Senesse P, Michiels C, Maingon P, Coudert B, Fraisse J, Queuniet A, Gasnault L, Gstach JH, Guichard B, Howaizi M, Geoffroy P, Picot C, Fournet J, Mousseau M, Stampfli C, Michel P, Doll J, Durand S, Buffet C, Triboulet JP, Denimal F, Hebbar M, Quandalle P, Mirabel X, Lledo G, Giovannini M, Souillac P, Untereiner M, Leroy-Terquem E, Lacroix H, Francois E, Lagasse JP, Breteau N, Legoux JL, Etienne JC, Delattre JF, Lubrano D, Levy-Chazal N, Palot JP, Nasca S, Demange L, Nguyen TD, Seng S, Michel P, Teniere P, Thevenet P, Le Brise H, Fleury J, Kammerer J, Cosme H, Novello P, Avignon JP, Berton C, Legueul, Parisot P, Aunis G, Vetter D, Platini C, Cals L, Rouhier D, Robin B, Champetier T, Cartalat A, Marchal C, Guillemin F, Flamenbaum M, Cassan D, Ducreux M., Hôpital Claude Huriez, Université de Lille, Droit et Santé-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), CHU - HÔTEL-DIEU Clermont-Ferrand, Service de chirurgie digestive [Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Institut de Cancérologie de Lorraine - Alexis Vautrin (ICL), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Université de Lille, Droit et Santé-Centre Hospitalier Régional Universitaire [Lille] ( CHRU Lille ), Hôpital Robert Debré-Centre Hospitalier Universitaire de Reims ( CHU Reims ), Maladies chroniques, santé perçue, et processus d'adaptation. Approches épidémiologiques et psychologiques. ( APEMAC - EA 4360 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Université de Lorraine ( UL ), Institut de Cancérologie de Lorraine - Alexis Vautrin ( ICL ), CHU de Poitiers, and Hôpital de la Timone [CHU - APHM] ( TIMONE )
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Male ,Cancer Research ,Time Factors ,Esophageal Neoplasms ,Kaplan-Meier Estimate ,MESH: Esophagectomy ,law.invention ,MESH: Proportional Hazards Models ,MESH : Adenocarcinoma ,0302 clinical medicine ,Randomized controlled trial ,law ,Risk Factors ,MESH : Esophagectomy ,MESH: Risk Factors ,MESH : Neoplasm Staging ,MESH : Female ,MESH : Carcinoma, Squamous Cell ,MESH: Treatment Outcome ,MESH: Chemoradiotherapy ,Randomised controlled trial ,education.field_of_study ,Hazard ratio ,[ SDV.SPEE ] Life Sciences [q-bio]/Santé publique et épidémiologie ,MESH: Carcinoma, Squamous Cell ,Chemoradiotherapy ,MESH : Chemoradiotherapy ,MESH: Neoplasm Staging ,MESH : Risk Factors ,Neoadjuvant Therapy ,3. Good health ,Oesophageal neoplasms ,Treatment Outcome ,Oncology ,Chemoradiation ,030220 oncology & carcinogenesis ,MESH: Esophageal Neoplasms ,Carcinoma, Squamous Cell ,030211 gastroenterology & hepatology ,Female ,Esophageal Squamous Cell Carcinoma ,France ,MESH : Time Factors ,medicine.medical_specialty ,MESH: Radiotherapy, Adjuvant ,MESH : Male ,Population ,MESH: Neoadjuvant Therapy ,Locally advanced ,MESH : Treatment Outcome ,Adenocarcinoma ,MESH : Radiotherapy, Adjuvant ,MESH : Kaplan-Meier Estimate ,03 medical and health sciences ,Early surgery ,medicine ,Humans ,Basal cell ,Salvage surgery ,education ,MESH : France ,Contraindication ,MESH: Kaplan-Meier Estimate ,Neoplasm Staging ,Proportional Hazards Models ,MESH: Humans ,business.industry ,MESH : Humans ,MESH: Adenocarcinoma ,MESH: Time Factors ,Cancer ,medicine.disease ,MESH : Proportional Hazards Models ,MESH: Male ,Surgery ,Esophagectomy ,MESH: France ,Radiotherapy, Adjuvant ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,MESH : Esophageal Neoplasms ,business ,MESH : Neoadjuvant Therapy ,MESH: Female - Abstract
International audience; BACKGROUND:Two randomised trials concerning thoracic oesophageal cancer concluded that for squamous cell carcinoma, chemoradiation alone leads to the same overall survival (OS) as chemoradiation followed by surgery. One of these trials, FFCD 9102, randomised only fit, compliant and operable responders to induction chemoradiation between continuation of chemoradiation and surgery. In the present analysis, the outcome in the patients not eligible for randomisation was calculated to determine if attempt of surgery should be recommended.METHODS:Eligible patients had operable T3-N0/N1-M0 thoracic oesophageal cancer. After initial chemoradiation, patients with no clinical response, or with contraindication to follow any attributed treatment, were not randomised. OS was studied first in the whole population of not randomised patients, and then specifically in clinical non-responders. The impact of surgery on OS was studied in these two populations.FINDINGS:Of the 451 registered patients in the trial, 192 were not randomised. Among them, 111 were clinical non-responders. Median OS was significantly shorter for non-randomised patients (11.5 months) than for randomised patients (18.9 months; p=0.0024). However, for the 112 non-randomised patients who underwent surgery, median OS was not different from that in randomised patients: 17.3 versus 18.9 months (p=0.58). Concerning clinical non-responders, median OS was longer for those who underwent surgery compared to non-operated patients: 17.0 versus 5.5 months (hazard ratio (HR)=0.39 [0.25-0.61]; p
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- 2015
13. Minimal influence of milling technique in contrast to sourdough on the nutritional and organoleptic quality of bread.
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Mietton L, Mata-Orozco J, Guezenec S, Marlin T, Samson MF, Canaguier E, Godet T, Nolleau V, Segond D, Cassan D, Baylet M, Bedouelle P, Bonnel L, Bouquin H, Christin G, Courteau M, Doucoure M, Hazard V, Kober T, Montard A, Nodet M, Parent M, Dalmasso C, Gainon A, Jouve O, Pichard S, Puel J, Simon R, Nidelet T, and Sicard D
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- Fermentation, Triticum metabolism, Edible Grain, Sensation, Bread analysis, Flour analysis
- Abstract
A number of studies have shown the effect of cereals and sourdough on bread nutritional and organoleptic quality, but the impact of the milling technique remains little studied. There are two main types of milling technic depending on the bread-making food chain. Industrial bakeries mainly use roller mills while artisanal bakeries may also use stone mill. We set up a participatory experiment with six millers and four bakers to study the impact of these two milling techniques on the quality of flours, sourdough microbiota and the quality of breads. Millers made twenty-two different flours from four different wheat grain varieties using either roller or stone mills. Each baker initiated and maintained sourdoughs with three roller-milled and three stone-milled flours during at least 32 backsloppings and then made bread. The analysis of flours revealed a typical granulometry profile linked to wheat hardness with higher particle sizes for stone-milled flours. Stone-milled flours also had a higher maltose content. However, the milling technic did not drive the composition of the sourdough microbiota. Moreover, the analysis of bread revealed that variation in bread protein fractions and in bread aroma compounds were more related to the specific baker microbial community than to the milling technique. Carbohydrate contents were clearly linked to the main LAB species metabolism. These results revealed that the sourdough microbial community shapes the organoleptic and nutritional quality of bread more than milling techniques., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)
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- 2024
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14. Physicochemical and Sensorial Characterization of Artisanal Pasta from the Occitanie Region in France.
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Belahcen L, Cassan D, Canaguier E, Robin MH, Chiffoleau Y, Samson MF, and Jard G
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Artisanal pasta made from wheat or underutilized cereal flours has grown in popularity with the expansion of the local and short food chains. Artisanal pasta makers do not use the same raw materials or production processes, leading to great variability in the final product. The purpose of the study is to determine the physicochemical and sensory characteristics of artisanal pasta made from durum wheat flour. Seven brands of fusilli pasta manufactured in the Occitanie region (France) were selected and analyzed in terms of their physicochemical composition (protein and ash content in dry samples), cooking properties (optimal cooking time, water absorption, and cooking loss), sensory characteristics (Pivot profile), and consumer appreciation. Differences in the physicochemical characteristics of the dry pasta samples partly explain the variations in pasta characteristics measured after cooking. The Pivot profile varied among pasta brands, but no major differences in hedonic properties were identified. To our knowledge, this is the first time that artisanal pasta made from flour has been characterized in terms of its physicochemical and sensory properties, which highlights the diversity of products on the market.
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- 2022
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15. Processing a 100% legume pasta in a classical extruder without agglomeration during mixing.
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Laleg K, Cassan D, Abecassis J, and Micard V
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- Humans, Nutritive Value, Temperature, Fabaceae chemistry, Food Handling
- Abstract
Pasta made exclusively from legume has high nutritional potential (rich in protein and gluten free). However, it is difficult to produce 100% legume dough suitable for the extrusion step in pasta production that comprises hydration, mixing, and extrusion. This paper addresses the biochemical phenomena at the origin of the agglomeration of dough particles frequently reported in the literature, which results in very sticky dough that cannot be extruded. We tested changes in mixing conditions including mixing temperature, addition of antioxidants, and flour pretreatment. Our results suggest that enzymatic reactions, notably lipoxygenase related redox activity, are responsible for this impairment of dough mixing and extrusion. Some of the process conditions studied can be applied at industrial scale and will help produce a legume food with nutritional and culinary qualities, beneficial for people with celiac disease, or gluten intolerance, as well as the general population. PRACTICAL APPLICATION: In the context of a sustainable and healthy food transition, the food industry is developing legume-based food of high nutritional quality that is widely consumed, like pasta. However, using legumes often leads to technological problems during the mixing and extrusion of pasta. This article demonstrates they are linked to enzymatic oxidative phenomena and provides an easy solution to reduce the problems without drastically changing pasta processing. Applied at industrial scale, it will allow the production of naturally gluten-free pasta rich in protein (two to three times the protein content of wheat pasta), of good nutritional quality., (© 2021 The Authors. Journal of Food Science published by Wiley Periodicals LLC on behalf of Institute of Food Technologists.)
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- 2021
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16. Vascularization and biocompatibility of poly(ε-caprolactone) fiber mats for rotator cuff tear repair.
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Gniesmer S, Brehm R, Hoffmann A, de Cassan D, Menzel H, Hoheisel AL, Glasmacher B, Willbold E, Reifenrath J, Ludwig N, Zimmerer R, Tavassol F, Gellrich NC, and Kampmann A
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- Animals, Biocompatible Materials therapeutic use, Capillaries physiology, Chitosan chemistry, Female, Macrophages cytology, Macrophages metabolism, Mice, Mice, Inbred BALB C, Microscopy, Fluorescence, Nanofibers chemistry, Porosity, Prostheses and Implants, Rotator Cuff blood supply, Rotator Cuff Injuries pathology, Biocompatible Materials chemistry, Microcirculation, Polyesters chemistry, Rotator Cuff Injuries therapy
- Abstract
Rotator cuff tear is the most frequent tendon injury in the adult population. Despite current improvements in surgical techniques and the development of grafts, failure rates following tendon reconstruction remain high. New therapies, which aim to restore the topology and functionality of the interface between muscle, tendon and bone, are essentially required. One of the key factors for a successful incorporation of tissue engineered constructs is a rapid ingrowth of cells and tissues, which is dependent on a fast vascularization. The dorsal skinfold chamber model in female BALB/cJZtm mice allows the observation of microhemodynamic parameters in repeated measurements in vivo and therefore the description of the vascularization of different implant materials. In order to promote vascularization of implant material, we compared a porous polymer patch (a commercially available porous polyurethane based scaffold from Biomerix™) with electrospun polycaprolactone (PCL) fiber mats and chitosan-graft-PCL coated electrospun PCL (CS-g-PCL) fiber mats in vivo. Using intravital fluorescence microscopy microcirculatory parameters were analyzed repetitively over 14 days. Vascularization was significantly increased in CS-g-PCL fiber mats at day 14 compared to the porous polymer patch and uncoated PCL fiber mats. Furthermore CS-g-PCL fiber mats showed also a reduced activation of immune cells. Clinically, these are important findings as they indicate that the CS-g-PCL improves the formation of vascularized tissue and the ingrowth of cells into electrospun PCL scaffolds. Especially the combination of enhanced vascularization and the reduction in immune cell activation at the later time points of our study points to an improved clinical outcome after rotator cuff tear repair., Competing Interests: The authors have declared that no competing interests exist.
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- 2020
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17. Possibilities and limitations of electrospun chitosan-coated polycaprolactone grafts for rotator cuff tear repair.
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Willbold E, Wellmann M, Welke B, Angrisani N, Gniesmer S, Kampmann A, Hoffmann A, de Cassan D, Menzel H, Hoheisel AL, Glasmacher B, and Reifenrath J
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- Aged, Animals, Biomechanical Phenomena, Humans, Male, Materials Testing, Orthopedic Procedures methods, Polymers chemistry, Polyurethanes chemistry, Porosity, Rats, Rats, Inbred Lew, Rotator Cuff Injuries pathology, Rupture pathology, Stress, Mechanical, Sutures, Tendons pathology, Wound Healing, Chitosan chemistry, Electrochemistry methods, Polyesters chemistry, Rotator Cuff surgery, Rotator Cuff Injuries surgery, Tissue Engineering methods, Tissue Scaffolds chemistry
- Abstract
Acute and chronic rotator cuff tears remain challenging for therapy. A wide range of therapeutic approaches were developed but re-tears and postoperative complications occur regularly. Especially in elderly people, the natural regeneration processes are decelerated, and graft materials are often necessary to stabilize the tendon-to-bone attachment and to improve the healing process. We here investigated in a small animal model a newly developed electrospun polycaprolactone fiber implant coated with a chitosan-polycaprolactone graft copolymer and compared these implants biomechanically and histologically with either a commercially available porous polyurethane implant (Biomerix 3D Scaffold) or suture-fixed tendons. Fifty-one rats were divided into three groups of 17 animals each. In the first surgery, the left infraspinatus tendons of all rats were detached, and the animals recovered for 4 weeks. In the second surgery, the tendons were fixed with suture material only (suture-fixed group; n = 17), whereas in the two experimental groups, the tendons were fixed with suture material and the polyurethane implant (Biomerix scaffold group; n = 17) or the modified electrospun polycaprolactone fiber implant (CS-g-PCL scaffold group; n=17), respectively. The unaffected right infraspinatus tendons were used as native controls. After a recovery of 8 weeks, all animals were clinically inconspicuous. In 12 animals of each group, repaired entheses were biomechanically tested for force at failure, stiffness, and modulus of elasticity, and in five animals, repaired entheses were analyzed histologically. Biomechanically, all parameters did not differ statistically significant between both implant groups, and the entheses failed typically at the surgical site. However, with respect to the force at failure, the median values of the two implant groups were smaller than the median value of the suture-fixed group. Histologically, the modified polycaprolactone fiber implant showed no acute inflammation processes, a good infiltration with cells, ingrowth of blood vessels and tendinous tissue, and a normal fibrous ensheathment. Further improvement of the implant material could be achieved by additional implementation of drug delivery systems. Therewith, the used CS-g-PCL fiber mat is a promising basic material to reach the goal of a clinically usable graft for rotator cuff tear repair., (© 2019 John Wiley & Sons, Ltd.)
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- 2020
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18. Mueller Matrix Measurement of Electrospun Fiber Scaffolds for Tissue Engineering.
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Fricke D, Becker A, Jütte L, Bode M, de Cassan D, Wollweber M, Glasmacher B, and Roth B
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Electrospun fiber scaffolds are gaining in importance in the area of tissue engineering. They can be used, for example, to fabricate graded implants to mimic the tendon bone junction. For the grading of the tensile strength of the fiber scaffolds, the orientation of the fibers plays a major role. This is currently measured by hand in scanning electron microscope (SEM) images. In this work, a correlation between polarimetric information generated by measuring the Mueller matrix (MM) and the orientation of the fibers of electrospun fiber scaffolds is reported. For this, the MM of fiber scaffolds, which were manufactured with different production parameters, was measured and analyzed. These data were correlated with fiber orientation and mechanical properties, which were evaluated in an established manner. We found that by measurement of the MM the production parameters as well as the relative orientation of the fibers in space can be determined. Thus, the MM measurement is suitable as an alternative tool for non-contact, non-destructive determination of the production parameters and, thus, the degree of alignment of electrospun fiber scaffolds., Competing Interests: The authors declare no conflict of interest.
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- 2019
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19. In vivo analysis of vascularization and biocompatibility of electrospun polycaprolactone fibre mats in the rat femur chamber.
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Gniesmer S, Brehm R, Hoffmann A, de Cassan D, Menzel H, Hoheisel AL, Glasmacher B, Willbold E, Reifenrath J, Wellmann M, Ludwig N, Tavassol F, Zimmerer R, Gellrich NC, and Kampmann A
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- Animals, Chitosan chemistry, Chitosan pharmacology, Femur blood supply, Femur pathology, Male, Porosity, Rats, Rats, Inbred Lew, Femur metabolism, Implants, Experimental, Materials Testing, Neovascularization, Physiologic, Polycarboxylate Cement chemistry, Polycarboxylate Cement pharmacology
- Abstract
In orthopaedic medicine, connective tissues are often affected by traumatic or degenerative injuries, and surgical intervention is required. Rotator cuff tears are a common cause of shoulder pain and disability among adults. The development of graft materials for bridging the gap between tendon and bone after chronic rotator cuff tears is essentially required. The limiting factor for the clinical success of a tissue engineering construct is a fast and complete vascularization of the construct. Otherwise, immigrating cells are not able to survive for a longer period of time, resulting in the failure of the graft material. The femur chamber allows the observation of microhaemodynamic parameters inside implants located in close vicinity to the femur in repeated measurements in vivo. We compared a porous polymer patch (a commercially available porous polyurethane-based scaffold from Biomerix™) with electrospun polycaprolactone (PCL) fibre mats and chitosan (CS)-graft-PCL modified electrospun PCL (CS-g-PCL) fibre mats in vivo. By means of intravital fluorescence microscopy, microhaemodynamic parameters were analysed repetitively over 20 days at intervals of 3 to 4 days. CS-g-PCL modified fibre mats showed a significantly increased vascularization at Day 10 compared with Day 6 and at Day 14 compared with the porous polymer patch and the unmodified PCL fibre mats at the same day. These results could be verified by histology. In conclusion, a clear improvement in terms of vascularization and biocompatibility is achieved by graft-copolymer modification compared with the unmodified material., (© 2019 The Authors Journal of Tissue Engineering and Regenerative Medicine Published by John Wiley & Sons Ltd.)
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- 2019
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20. Impact of sterilization by electron beam, gamma radiation and X-rays on electrospun poly-(ε-caprolactone) fiber mats.
- Author
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de Cassan D, Hoheisel AL, Glasmacher B, and Menzel H
- Subjects
- Electrons, Electroplating methods, Gamma Rays, Humans, Materials Testing, Microscopy, Electron, Scanning, Polyesters radiation effects, Polymers chemistry, Polymers radiation effects, Sterilization methods, Stress, Mechanical, Surface Properties, Tissue Engineering methods, X-Rays, Disinfection methods, Membranes, Artificial, Nanofibers chemistry, Nanofibers radiation effects, Polyesters chemistry, Tissue Scaffolds chemistry
- Abstract
Biodegradable polymers such as polycaprolactone (PCL) are increasingly used for electrospinning substrates for tissue engineering. These materials offer great advantages such as biocompatibility and good mechanical properties. However, in order to be approved for human implantation they have to be sterilized. The impact of commonly used irradiation sterilization methods on electrospun PCL fiber mats was investigated systematically. Electron beam (β-irradiation), gamma and X-ray irradiation with two different doses (25 and 33 kGy) were investigated. To determine the impact on the fiber mats, mechanical, chemical, thermal properties and crystallinity were investigated. Irradiation resulted in a significant decrease in molecular weight. At the same time, crystallinity of fiber mats increased significantly. However, the mechanical properties did not change significantly upon irradiation, mostly likely because effects of a lower molecular weight were balanced with the higher degree of crystallinity. The irradiation effects were dose dependent, a higher irradiation dose led to stronger changes. Gamma irradiation seemed to be the least suited method, while electron beams (β irradiation) had a lower impact. Therefore, β irradiation is recommended as sterilization method for electrospun PCL fiber mats.
- Published
- 2019
- Full Text
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21. Layer-by-layer deposition of chitosan nanoparticles as drug-release coatings for PCL nanofibers.
- Author
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Sydow S, de Cassan D, Hänsch R, Gengenbach TR, Easton CD, Thissen H, and Menzel H
- Subjects
- Alginates chemistry, Chitosan chemistry, Chondroitin Sulfates chemistry, Drug Delivery Systems, Drug Liberation, Humans, Hyaluronic Acid chemistry, Nanofibers ultrastructure, Nanoparticles ultrastructure, Transforming Growth Factor beta3 chemistry, Chitosan analogs & derivatives, Coated Materials, Biocompatible chemistry, Drug Carriers chemistry, Nanofibers chemistry, Nanoparticles chemistry, Polyesters chemistry, Transforming Growth Factor beta3 administration & dosage
- Abstract
Nanogels were prepared by ionotropic gelation of chitosan (CS) with tripolyphosphate (TPP). The use of such nanogels to prepare coatings by layer-by-layer deposition (LbL) was studied. The nanogels were characterized in terms of particle size, zeta-potential and stability. Nanogel suspensions were used to build polyelectrolyte multilayers on silicon wafers and on PCL fiber mats by LbL-deposition. Three different polysaccharides were used as polyanions, namely chondroitin sulfate, alginate and hyaluronic acid. The ellipsometric thickness was demonstrated to depend significantly on the type of polyanion. XPS analysis with depth profiling further substantiated the differences in the chemical composition of the films with the different polyanions. Furthermore, XPS data clearly indicated a strong penetration of the polyanions into the CS-TPP layer, resulting in a complete exchange and release of the TPP ions. The LbL-deposition also was studied with PCL fiber mats, which were modified with a chitosan-PCL-graft polymer and alginate. The possibility to create graded coatings on the fiber mats was shown employing fluorescently labelled CS-TPP nanoparticles. The potential of the coatings as drug delivery system for therapeutic proteins was exemplified with the release of Transforming Growth Factor β3 (TGF-β3). The CS-TPP nanogels were shown to encapsulate and release therapeutic proteins. In combination with the layer-by-layer deposition they will allow the creation of PCL fiber mat implants having with drug gradients for applications at tissue transitions.
- Published
- 2018
- Full Text
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22. Attachment of nanoparticulate drug-release systems on poly(ε-caprolactone) nanofibers via a graftpolymer as interlayer.
- Author
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de Cassan D, Sydow S, Schmidt N, Behrens P, Roger Y, Hoffmann A, Hoheisel AL, Glasmacher B, Hänsch R, and Menzel H
- Subjects
- Cell Death drug effects, Chitosan chemistry, Crystallization, Drug Delivery Systems, Humans, Nanofibers ultrastructure, Polyesters chemical synthesis, Proton Magnetic Resonance Spectroscopy, Water chemistry, Wettability, Ciprofloxacin pharmacology, Drug Liberation, Nanofibers chemistry, Nanoparticles chemistry, Polyesters chemistry
- Abstract
Electrospun poly(ε-caprolactone) (PCL) fiber mats are modified using a chitosan grafted with PCL (CS-g-PCL), to improve the biological performance and to enable further modifications. The graft copolymer is immobilized by the crystallization of the PCL grafts on the PCL fiber surface as binding mechanism. In this way, the surface of the fibers is covered with chitosan bearing cationic amino groups, which allow adsorption of oppositely charged nanoparticulate drug-delivery systems. The modification of the fiber mats and the attachment of the drug delivery systems are easy and scalable dip processes. The process is also versatile; it is possible to attach different polymeric and inorganic nanoparticulate drug-release systems of cationic or anionic nature. The modifications are verified using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). As proof of principle, the release of ciprofloxacin from silica nanoparticles attached to the modified fiber mats is shown; however, the method is also suited for other biologically active substances including growth factors. The initial cellular attachment and proliferation as well as vitality of the cells is improved by the modification with CS-g-PCL and is further influenced by the type of the drug delivery system attached. Hence, this method can be used to transfer PCL fiber mats into bioactive implants for in-situ tissue engineering applications., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
- Full Text
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23. Structural, Culinary, Nutritional and Anti-Nutritional Properties of High Protein, Gluten Free, 100% Legume Pasta.
- Author
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Laleg K, Cassan D, Barron C, Prabhasankar P, and Micard V
- Subjects
- Cooking, Diet, Gluten-Free, Dietary Fiber analysis, Glutens adverse effects, Glutens chemistry, Glycemic Index, Humans, Starch chemistry, Starch pharmacology, Triticum adverse effects, Triticum chemistry, Digestion, Fabaceae chemistry, Flour analysis, Nutritive Value
- Abstract
Wheat pasta has a compact structure built by a gluten network entrapping starch granules resulting in a low glycemic index, but is nevertheless unsuitable for gluten-intolerant people. High protein gluten-free legume flours, rich in fibers, resistant starch and minerals are thus a good alternative for gluten-free pasta production. In this study, gluten-free pasta was produced exclusively from faba, lentil or black-gram flours. The relationship between their structure, their cooking and Rheological properties and their in-vitro starch digestion was analyzed and compared to cereal gluten-free commercial pasta. Trypsin inhibitory activity, phytic acid and α-galactosides were determined in flours and in cooked pasta. All legume pasta were rich in protein, resistant starch and fibers. They had a thick but weak protein network, which is built during the pasta cooking step. This particular structure altered pasta springiness and increased cooking losses. Black-gram pasta, which is especially rich in soluble fibers, differed from faba and lentil pasta, with high springiness (0.85 vs. 0.75) and less loss during cooking. In comparison to a commercial cereal gluten-free pasta, all the legume pasta lost less material during cooking but was less cohesive and springy. Interestingly, due to their particular composition and structure, lentil and faba pasta released their starch more slowly than the commercial gluten-free pasta during the in-vitro digestion process. Anti-nutritional factors in legumes, such as trypsin inhibitory activity and α-galactosides were reduced by up to 82% and 73%, respectively, by pasta processing and cooking. However, these processing steps had a minor effect on phytic acid. This study demonstrates the advantages of using legumes for the production of gluten-free pasta with a low glycemic index and high nutritional quality., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2016
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24. Enrichment of pasta with faba bean does not impact glycemic or insulin response but can enhance satiety feeling and digestive comfort when dried at very high temperature.
- Author
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Greffeuille V, Marsset-Baglieri A, Molinari N, Cassan D, Sutra T, Avignon A, and Micard V
- Subjects
- Adult, Digestion, Female, Flour analysis, Food Additives chemistry, Food Handling, Hot Temperature, Humans, Male, Temperature, Triticum chemistry, Vicia faba chemistry, Young Adult, Blood Glucose metabolism, Food Additives metabolism, Insulin metabolism, Satiation, Triticum metabolism, Vicia faba metabolism
- Abstract
Enrichment of durum wheat pasta with legume flour enhances their protein and essential amino acid content, especially lysine content. However, despite its nutritional potential, the addition of a legume alters the rheological properties of pasta. High temperature drying of pasta reduces this negative effect by strengthening its protein network. The aim of our study was to determine if these changes in the pasta structure alter its in vitro carbohydrate digestibility, in vivo glycemic, insulin and satiety responses. We also investigated if high temperature drying of pasta can reduce the well-known digestive discomfort associated with the consumption of legume grains. Fifteen healthy volunteers consumed three test meals: durum wheat pasta dried at a low temperature (control), and pasta enriched with 35% faba bean dried at a low and at a very high temperature. When enriched with 35% legume flour, pasta maintained its nutritionally valuable low glycemic and insulin index, despite its weaker protein network. Drying 35% faba bean pasta at a high temperature strengthened its protein network, and decreased its in vitro carbohydrate digestion with no further decrease in its in vivo glycemic or insulin index. Drying pasta at a very high temperature reduced digestive discomfort and enhanced self-reported satiety, and was not associated with a modification of energy intake in the following meal.
- Published
- 2015
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25. Blood rheology and body composition as determinants of exercise performance in female rugby players.
- Author
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Brun JF, Varlet-Marie E, Cassan D, and Raynaud de Mauverger E
- Subjects
- Adiposity physiology, Adult, Erythrocyte Deformability physiology, Female, Hand Strength, Humans, Oxygen Consumption physiology, Young Adult, Athletic Performance physiology, Body Composition physiology, Football physiology, Hemorheology physiology
- Abstract
Athletes involved in rugby are characterized by a very specific pattern of body composition with an unusually important muscle mass. In a preceding study about rugbymen we evidenced that they exhibit a correlation between red blood cell aggregability and the amount of body fat although it remains within a normal range, and that red cell rigidity was correlated to isometric adductor strength. We had the opportunity of studying the relationships among exercise performance, body composition and hemorheology in 19 female rugby players (age 19-26, mean: 24.47 ± 0.67 yr) practising 4 - 10 hr/wk (mean 7.15 ± 0.3) since 1-12 yr (mean 4,05 ± 0,694). VO2max was not related by its own to blood rheology, either hematocrit (r = -0.0717 p = 0.7706) or plasma viscosity (r = 0.0144; p = 0.9533), but other markers of performance exhivited a correlation with red cell rheology. Relationships between fitness and body composition were evidenced. Isometric handgrip strength was negatively correlated to red blood cell aggregability (Myrenne M, r = -0.57839; p = 0.00948 M1 r = -0.58910; p = 0.00795). Adductor isometric strength was negatively correlated to red blood cell aggregability Myrenne M (r = -0.5033; p = 0.0280) but not to M1 (r = -0.4227; p = 0.0714). Fat mass is a major determinant of the maximal oxygen consumption VO2max either measured by a field test (r = -0.766; p = 0.00013) or exercise test (r = -0.575; p = 0.00994) and was also negatively correlated to both handgrip (r = -0.4918; p = 0.0325) and RBC aggregability M (r = -0.57839; p = 0.00948 and M1 r = -0.5891; p = 0.00795). Independently of fat mass, FFM appears to be a determinant of blood viscosity (r = 0.4622; p = 0.0463) due to its correlation with RBC rigidity (r = 0.4781; p = 0.0384). Thus, trained young women exercising 4-10 hr/wk and thus exhibiting a low percentage of body fat exhibit clear relationships between body composition and hemorheology, but fat mass being low, the parameter correlated with blood rheology is in this case fat-free mass, consistent with recent findings indicating that high fat mass in women is sometimes correlated with parameters of the metabolic syndrome such as insulin resistance or inflammation. In addition, parameters quantifying fatness even within such a physiological range are in this sample negatively related with exercise performance.
- Published
- 2011
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26. Prevalence and clinical implications of anemia in congestive heart failure patients followed at a specialized heart function clinic.
- Author
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Elabbassi W, Fraser M, Williams K, Cassan D, and Haddad H
- Subjects
- Aged, Anemia etiology, Comorbidity, Female, Heart Failure complications, Heart Failure mortality, Humans, Male, Middle Aged, Ontario epidemiology, Prevalence, Risk Assessment, Risk Factors, Survival, Anemia epidemiology, Cardiac Care Facilities, Heart Failure epidemiology
- Abstract
The objective of this study was to assess the prevalence of anemia and to investigate its association with comorbidities and its impact on clinical outcomes in patients with heart failure. The association of predefined anemia, as well as the correlation of serum hemoglobin level as a continuous variable, with outcomes of emergency department visits, hospitalization, and mortality was investigated. There were fewer anemic patients in New York Heart Association classes I and II than in classes III and IV. Anemia was associated with higher rates of emergency department visits, hospital admissions, and all-cause mortality. Multivariable analysis showed that anemia is independently associated with mortality. When hemoglobin level was considered as a continuous variable, the authors noted that the mortality risk correlated with hemoglobin level disappears when hemoglobin level exceeds 140 g/L. The authors conclude that anemia has strong impacts on functional class and other clinical outcomes in patients with heart failure.
- Published
- 2006
- Full Text
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27. Effects of medium-pressure UV lamps radiation on water quality in a chlorinated indoor swimming pool.
- Author
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Cassan D, Mercier B, Castex F, and Rambaud A
- Subjects
- Hydrocarbons, Chlorinated radiation effects, Water analysis, Water Pollutants, Chemical radiation effects, Water Purification instrumentation, Hydrocarbons, Chlorinated analysis, Swimming Pools standards, Ultraviolet Rays, Water standards, Water Pollutants, Chemical analysis, Water Purification standards
- Abstract
The aim of our study was to determine the impact of medium-pressure UV lamps radiation on water quality in a chlorinated indoor swimming pool. An indoor swimming pool was equipped with two medium-pressure UV lamps. We collected eight samples of water daily over a four-weeks period and measured total and free chlorine, pH, water temperature, bacteriological parameters, total organic carbon and trihalomethanes. During the first week, which served as control, medium-pressure UV lamps were turned off. During the next three weeks, medium-pressure UV lamps were kept on 24 h per day. The third week, we reduced the level of the injected chlorine into water, and the last week we also reduced the water renewal volume by 27%. Our results showed that bacteriological parameters remained within allowable french limits. When medium-pressure UV lamps were kept on, total, free and active chlorine levels were significantly increased (P<0.001), whereas combined chlorine level were significantly decreased (P<0.001 and P<0.05, respectively). The levels of chloroform and bromodichloromethane were significantly increased when medium-pressure UV lamps were kept on (P<0.001), whereas chlorodibromomethane and bromoform levels significantly decreased (P<0.05 and P<0.001, respectively). The additional formation of chloroform and bromodichloromethane may be explained by the increase in active chlorine and by radicalizing mechanisms initiated by UV radiation.
- Published
- 2006
- Full Text
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28. Contributions of social influences and psychopathological factors to cannabis use and dependence in high-school students.
- Author
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Chabrol H, Rey A, Cassan D, Julliot M, Carlin E, and Rodgers R
- Abstract
Objectives: To evaluate the relative contributions of peers cannabis use or non use, parental approval of such use, adolescents' own beliefs about use, self-esteem, and depressive and symptoms of borderline personality disorder, in the prediction of cannabis use and dependence., Method: Participants were 257 high-school students who completed questionnaires assessing cannabis use frequency, cannabis dependence, the number of peers using cannabis, the number of peers opposed to cannabis use, parental attitude toward cannabis use, participants' attitude toward use, self-esteem, depressive and borderline symptomatology. Logistic regression analyses were conducted to predict cannabis use, daily use and dependence., Results: The number of peers using cannabis, participants' approval of cannabis use, and the intensity of symptoms of borderline personality disorder were risk factors for use whereas the number of peers opposed to cannabis use was a protective factor. Parental attitudes toward use and participants' depressive symptomatology were not significant independent predictors of use. There were no significant predictors of probable cannabis dependence., Conclusion: To inform adolescents that a significant proportion of them disapprove of cannabis use may be an effective component of prevention of use. Symptoms of borderline personality disorder may account for the association between depression and cannabis use. The social influences and psychopathological factors evaluated in this study did not appear to play a major role in the development of cannabis dependence.
- Published
- 2005
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29. Blood fluidity is related to the ability to oxidize lipids at exercise.
- Author
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Brun JF, Varlet-Marie E, Cassan D, Manetta J, and Mercier J
- Subjects
- Erythrocyte Aggregation, Humans, Models, Biological, Oxidation-Reduction, Exercise physiology, Hemorheology, Lipids blood
- Abstract
We previously reported in rugbywomen correlations between RBC deformability and the ability to oxidize at exercise more lipids. This surprising finding might of course be spurious, or reflect the importance of the balance of substrates at exercise on baseline parameters that regulate blood rheology. Actually, the capacity of skeletal muscle to utilize either lipid or carbohydrate as fuels strongly influences whole body metabolism both at rest and during exercise. While the healthy skeletal muscle has substantial metabolic flexibility and is able to switch from predominantly lipid o oxidation during fasting or endurance exercise to increased glucose oxidation in conditions of insulin stimulation, obese individuals and those with type 2 diabetes manifest higher lipid oxidation during insulin-stimulated conditions despite lower rates of lipid oxidation during fasting or prolonged exercise. A low ability to oxidize and to periodically deplete triglyceride in muscle is associated with raised blood lipids. In addition, high carbohydrate oxidation rates in the mitochondrion are likely to promote more free radical generation. An increase in either blood lipids or free radicals is likely to induce profound hemorheological effects. We present here hemorheological studies in various populations with the use of exercise calorimetry in order to assess this switch of substrates. These studies further evidence negative correlations between the ability to oxidize lipids at exercise and parameters of blood viscosity. Correlations found between RBC deformability and the ability to oxidize at exercise more lipids may be due to effects of endurance training on lipid oxidation which may in turn modify both lipid metabolism and free radical generation, thus influencing RBC rheology., (Copyright 2004 IOS Press)
- Published
- 2004
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