Search

Your search keyword '"Casciati, Arianna"' showing total 125 results
Start Over Author "Casciati, Arianna"
125 results on '"Casciati, Arianna"'

1. Comparing the effects of irradiation with protons or photons on neonatal mouse brain: Apoptosis, oncogenesis and hippocampal alterations

Author

Giovannini, Daniela, Antonelli, Francesca, Casciati, Arianna, De Angelis, Cinzia, Astorino, Maria Denise, Bazzano, Giulia, Fratini, Emiliano, Ampollini, Alessandro, Vadrucci, Monia, Cisbani, Evaristo, Nenzi, Paolo, Picardi, Luigi, Saran, Anna, Marino, Carmela, Mancuso, Mariateresa, Ronsivalle, Concetta, and Pazzaglia, Simonetta

Published
2024
Full Text
View/download PDF

4. Lab-on-Chip Label-Free Sensing System for Electroporation Based on Time-Lapse Microscopy

Author

Antonelli, Gianni, Camera, Francesca, Mencattini, Arianna, Casciati, Arianna, Tanori, Mirella, Zambotti, Alessandro, Casti, Paola, Curci, Giorgia, Filippi, Joanna, D'Orazio, Michele, Merla, Caterina, and Martinelli, Eugenio

Abstract

Electroporation has been shown to have considerable potential in biological research and clinical applications. This technique permits the opening of reversible or irreversible nanometric pores in cellular membranes by the application of a high-intensity electric field characterized by extremely short duration (i.e., microseconds, nanoseconds down to tens of picoseconds). Recently, also thanks to the spreading use of lab-on-chip, it was discovered that applying a high electric field to single cells can cause them to contract and generate blebs. Even though these effects are known in the literature, their correlation with the electroporation phenomenon is still unclear. For this reason, in this work, we present a reliable, low-cost sensing system based on a lab-on-chip platform with transferred laser-induced graphene (LIG) electrodes to study electroporation by bright-field and fluorescence time-lapse microscopy. The acquired video was then used to extrapolate the calcium intake due to electroporation, cell contractility, and morphology over time. The two abovementioned side effects were finally correlated with electroporation and used to estimate its efficacy on single cells, exploiting calcium intake information as the ground truth. The proposed sensing system could be used to predict the electroporation phenomenon easily and even in a label-free modality, using exclusively the morphological variations induced in the single cells.

Published
2024
Full Text
View/download PDF

5. Dosimetry for repetitive transcranial magnetic stimulation: a translational study from Alzheimer's disease patients to controlled in vitro investigations.

Author

Camera, Francesca, Colantoni, Eleonora, Casciati, Arianna, Tanno, Barbara, Mencarelli, Lucia, Di Lorenzo, Francesco, Bonnì, Sonia, Koch, Giacomo, and Merla, Caterina

Subjects
ALZHEIMER'S patients, TRANSCRANIAL magnetic stimulation, MEDICAL dosimetry, ALZHEIMER'S disease, ELECTRIC fields, CELL culture
Abstract

Objective. Recent studies have indicated that repetitive transcranial magnetic stimulation (rTMS) could enhance cognition in Alzheimer's Disease (AD) patients, but to now the molecular-level interaction mechanisms driving this effect remain poorly understood. While cognitive scores have been the primary measure of rTMS effectiveness, employing molecular-based approaches could offer more precise treatment predictions and prognoses. To reach this goal, it is fundamental to assess the electric field (E-field) and the induced current densities (J) within the stimulated brain areas and to translate these values to in vitro systems specifically devoted in investigating molecular-based interactions of this stimulation. Approach. This paper offers a methodological procedure to guide dosimetric assessment to translate the E-field induced in humans (in a specific pilot study) into in vitro settings. Electromagnetic simulations on patients' head models and cellular holders were conducted to characterize exposure conditions and determine necessary adjustments for in vitro replication of the same dose delivered in humans using the same stimulating coil. Main results. Our study highlighted the levels of E-field and J induced in the target brain region and showed that the computed E-field and J were different among patients that underwent the treatment, so to replicate the exposure to the in vitro system, we have to consider a range of electric quantities as reference. To match the E-field to the levels calculated in patients' brains, an increase of at least the 25% in the coil feeding current is necessary when in vitro stimulations are performed. Conversely, to equalize current densities, modifications in the cells culture medium conductivity have to be implemented reducing it to one fifth of its value. Significance. This dosimetric assessment and subsequent experimental adjustments are essential to achieve controlled in vitro experiments to better understand rTMS effects on AD cognition. Dosimetry is a fundamental step for comparing the cognitive effects with those obtained by stimulating a cellular model at an equal dose rigorously evaluated. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

6. Involvement of Mitochondria in the Selective Response to Microsecond Pulsed Electric Fields on Healthy and Cancer Stem Cells in the Brain

Author

Casciati, Arianna, primary, Taddei, Anna Rita, additional, Rampazzo, Elena, additional, Persano, Luca, additional, Viola, Giampietro, additional, Cani, Alice, additional, Bresolin, Silvia, additional, Cesi, Vincenzo, additional, Antonelli, Francesca, additional, Mancuso, Mariateresa, additional, Merla, Caterina, additional, and Tanori, Mirella, additional

Published
2024
Full Text
View/download PDF

7. On the effects of 30.5 GHz sinusoidal wave exposure on glioblastoma organoids.

Author

Rampazzo, Elena, Persano, Luca, Karim, Nissar, Hodgking, George, Pinto, Rosanna, Casciati, Arianna, Tanori, Mirella, Zambotti, Alessandro, Bresolin, Silvia, Cani, Alice, Pannicelli, Alessandro, Davies, Ilan W., Hancock, Cristopher, Palego, Cristiano, Viola, Giampietro, Mancuso, Mariateresa, and Merla, Caterina

Subjects
GLIOBLASTOMA multiforme, BRAIN tumors, MILLIMETER waves, ORGANOIDS, ROOT-mean-squares, MEDICAL dosimetry
Abstract

Introduction: Glioblastoma (grade IV) is the most aggressive primary brain tumor in adults, representing one of the biggest therapeutic challenges due to its highly aggressive nature. In this study, we investigated the impact of millimeter waves on tridimensional glioblastoma organoids derived directly from patient tumors. Our goal was to explore novel therapeutic possibilities in the fight against this challenging disease. Methods: The exposure setup was meticulously developed in-house, and we employed a comprehensive dosimetry approach, combining numerical and experimental methods. Biological endpoints included a global transcriptional profiling analysis to highlight possible deregulated pathways, analysis of cell morphological changes, and cell phenotypic characterization which are all important players in the control of glioblastoma progression. Results and discussion: Our results revealed a significant effect of continuous millimeter waves at 30.5 GHz on cell proliferation and apoptosis, although without affecting the differentiation status of glioblastoma cells composing the organoids. Excitingly, when applying a power level of 0.1 W (Root Mean Square), we discovered a remarkable (statistically significant) therapeutic effect when combined with the chemotherapeutic agent Temozolomide, leading to increased glioblastoma cell death. These findings present a promising interventional window for treating glioblastoma cells, harnessing the potential therapeutic benefits of 30.5 GHz CW exposure. Temperature increase during treatments was carefully monitored and simulated with a good agreement, demonstrating a negligible involvement of the temperature elevation for the observed effects. By exploring this innovative approach, we pave the way for improved future treatments of glioblastoma that has remained exceptionally challenging until now. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

8. Characterization of Glioblastoma Organoid Bio-Responses Upon 30.5 GHz Continuous Wave Exposure

Author

Rampazzo, Elena, primary, Karim, Nissar, additional, Persano, Luca, additional, Pinto, Rosanna, additional, Casciati, Arianna, additional, Tanori, Mirella, additional, Hodgkins, George, additional, Davies, Ilan W., additional, Zambotti, Alessandro, additional, Hancock, Cristopher, additional, Palego, Cristiano, additional, Viola, Gimapietro, additional, Mancuso, Mariateresa, additional, and Merla, Caterina, additional

Published
2023
Full Text
View/download PDF

12. Gut–Brain Axis: Insights from Hippocampal Neurogenesis and Brain Tumor Development in a Mouse Model of Experimental Colitis Induced by Dextran Sodium Sulfate

Author

Vitali, Roberta, primary, Prioreschi, Clara, additional, Lorenzo Rebenaque, Laura, additional, Colantoni, Eleonora, additional, Giovannini, Daniela, additional, Frusciante, Sarah, additional, Diretto, Gianfranco, additional, Marco-Jiménez, Francisco, additional, Mancuso, Mariateresa, additional, Casciati, Arianna, additional, and Pazzaglia, Simonetta, additional

Published
2022
Full Text
View/download PDF

15. Effects of Ultra-Short Pulsed Electric Field Exposure on Glioblastoma Cells

Author

Casciati, Arianna, primary, Tanori, Mirella, additional, Gianlorenzi, Isabella, additional, Rampazzo, Elena, additional, Persano, Luca, additional, Viola, Giampietro, additional, Cani, Alice, additional, Bresolin, Silvia, additional, Marino, Carmela, additional, Mancuso, Mariateresa, additional, and Merla, Caterina, additional

Published
2022
Full Text
View/download PDF

18. SUMCASTEC_200621_NA_BIOEM 2020_Conference_Paper_pdf_Oxford_M.Tanori_Public_NA

Author

Tanori, Mirella, Casciati, Arianna, Zambotti, Alessandro, Pinto, Rosanna, Marino, Carmela, Mariateresa Mancuso, and Merla, Caterina

Subjects
stomatognathic diseases, neoplasms, nervous system diseases
Abstract

Ultrashort pulsed electric fields as specific radiosensitizers of medulloblastoma cancer stem cells paper accepted to presentation to BIOEM2020 conference

Published
2020
Full Text
View/download PDF

19. Immature cancer cell discrimination using combined high frequency Electromagnetic Fields and dielectrophoresis forces

Author

Dalmay, Claire, Manczak, Rémi, Saada, Sofiane, Provent, Thomas, Bessette, Barbara, Lalloué, Fabrice, Casciati, Arianna, Tanori, Mirella, Merla, Caterina, Mancuso, Mariateresa, Porcù, Elena, Rampazzo, Elena, Persano, Luca, Viola, Giampetro, Baristiran Kaynak, Canan, Kaynak, Mehmet, Palego, Cristiano, Pothier, Arnaud, RF-ELITE : RF-Electronique Imprimée pour les Télécommunications et l'Energie (XLIM-RFEI), XLIM (XLIM), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS)-Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Contrôle de l’Activation Cellulaire, Progression Tumorale et Résistance thérapeutique (CAPTuR), Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Agenzia Nazionale per le nuove Tecnologie, l’energia e lo sviluppo economico sostenibile (ENEA), Universita degli Studi di Padova, Innovations for High Performance Microelectronics (IHP), and Bangor University

Subjects
[SPI.ELEC]Engineering Sciences [physics]/Electromagnetism, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020

20. Human Medulloblastoma Cell Lines: Investigating on Cancer Stem Cell-Like Phenotype

Author

Casciati, Arianna, primary, Tanori, Mirella, additional, Manczak, Rémi, additional, Saada, Sofiane, additional, Tanno, Barbara, additional, Giardullo, Paola, additional, Porcù, Elena, additional, Rampazzo, Elena, additional, Persano, Luca, additional, Viola, Giampietro, additional, Dalmay, Claire, additional, Lalloué, Fabrice, additional, Pothier, Arnaud, additional, Merla, Caterina, additional, and Mancuso, Mariateresa, additional

Published
2020
Full Text
View/download PDF

21. SUMCASTEC_190914_NA_IJMWT_Journal paper_.pdf_Bath_I. Davies_Public_NA

Author

Davies, Ilan Wyn, Merla, Caterina, Casciati, Arianna, Tanori, Mirella, Zambotti, Alessandro, Mariateresa Mancuso, Bishop, John, White, Malcom, Palego, Cristiano, and Hancock, Christopher

Subjects
Circuit design and applications, EM field theory and numerical techniques, medical and biological effects, passive components and circuits, Si-based devices and IC technologie
Abstract

International Journal of Microwave and Wireless Technologies (JMWT) paper entitled "Push–pull configuration of high-power MOSFETs for generation of nanosecond pulses for electropermeabilization of cells". Accepted in April 2019., The published version DOI is: https://doi.org/10.1017/ S1759078719000576

Published
2019
Full Text
View/download PDF

27. SUMCASTEC_181003_NA_EMF-Med 2018 Pres_Conference presentation_.pdf_Limoges_A.Pothier_Public_NA

Author

Manczak, Rémi, Saada, Sofiane, Tanori, Mirella, Casciati, Arianna, Dalmay, Claire, Bessette, Barbara, Begaud, Gaelle, Battu, Serge, Blondy, Pierre, Jauberteau, Marie Odile, Baristiran Kaynak, Canan, Kaynak, Mehmet, Palego, Cristiano, Merla, Caterina, Tanno, Barbara, Mancuso, Mariateresa, Lalloue, Fabrice, and Pothier, Arnaud

Subjects
DEP discrimination, differentiated vs undifferentiated, medulloblastoma cells
Abstract

Presentation of conference paper entitled "High-Frequency Dielectrophoresis Characterizationof Differentiated vs UndifferentiatedMedulloblastoma Cells" at the 1st EMF-Med World Conference on Biomedical Applications of Electromagnetic Fields. Split, Croatia. Presented by Dr A. Pothier on10thSeptember 2018.

Published
2018
Full Text
View/download PDF

28. SUMCASTEC_181003_NA_EMF-Med 2018 Paper_Conference paper_.pdf_Bangor_C. Palego_Public_Last Draft

Author

Gamal, Wesam, Pinto, Rosanna, Tanori, Mirella, Casciati, Arianna, Mancuso, Mariateresa, Persano, Luca, Viola, Giampietro, Saada, Sofiane, Bessette, Barbara, Lalloue, Fabrice, Pothier, Arnaud, Palego, Cristiano, and Merla, Caterina

Subjects
Permittivity, Cancer Stem Cells, Electromagnetic Stimulation
Abstract

Conference paper entitled "Permittivity measurements of brain cancer cell towards the electric characterisation of single Cancer Stem Cells" accepted in the 1st EMF-Med World Conference on Biomedical Applications of Electromagnetic Fields. Split, Croatia, 10-13 September 2018., Bangor University also gratefully acknowledges the financial support provided by the Welsh Government and Higher Education Funding Council for Wales through the Sêr Cymru National Research Network in Advanced Engineering and Materials.

Published
2018
Full Text
View/download PDF

29. Permittivity measurements of brain cancer cell solutions: towards the electric characterisation of single cancer stem cells

Author

Gamal, Wesam, Tanori, Mirella, Casciati, Arianna, Mancuso, Mariateresa, Persano, Luca, Viola, Giampetro, Saada, Sofiane, Bessette, Barbara, Lalloué, Fabrice, Pothier, Arnaud, Palego, Cristiano, Merla, Caterina, RF-ELITE : RF-Electronique Imprimée pour les Télécommunications et l'Energie (XLIM-RFEI), XLIM (XLIM), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS)-Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Agenzia Nazionale per le nuove Tecnologie, l’energia e lo sviluppo economico sostenibile (ENEA), Universita degli Studi di Padova, Contrôle de l’Activation Cellulaire, Progression Tumorale et Résistance thérapeutique (CAPTuR), Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), and Bangor University

Subjects
[SPI]Engineering Sciences [physics], [SPI.ELEC]Engineering Sciences [physics]/Electromagnetism, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

31. High-Frequency Dielectrophoresis Characterization of Differentiated vs Undifferentiated Medulloblastoma Cells

Author

Manczak, Remi, primary, Saada, Sofiane, additional, Tanori, Mirella, additional, Casciati, Arianna, additional, Dalmay, Claire, additional, Bessette, Barbara, additional, Begaud, Gaelle, additional, Battu, Serge, additional, Blondy, Pierre, additional, Jauberteau, Marie Odile, additional, Kaynak, Canan Baristiran, additional, Kaynak, Mehmet, additional, Palego, Cristiano, additional, Merla, Caterina, additional, Tanno, Barbara, additional, Mancuso, Mariateresa, additional, Lalloue, Fabrice, additional, and Pothier, Arnaud, additional

Published
2018
Full Text
View/download PDF

33. Synthetic lethal genetic interactions between Rad54 and PARP-1 in mouse development and oncogenesis

Author

Tanori, Mirella, primary, Casciati, Arianna, additional, Berardinelli, Francesco, additional, Leonardi, Simona, additional, Pasquali, Emanuela, additional, Antonelli, Francesca, additional, Tanno, Barbara, additional, Giardullo, Paola, additional, Pannicelli, Alessandro, additional, Babini, Gabriele, additional, De Stefano, Ilaria, additional, Sgura, Antonella, additional, Mancuso, Mariateresa, additional, Saran, Anna, additional, and Pazzaglia, Simonetta, additional

Published
2016
Full Text
View/download PDF

34. Age-related effects of X-ray irradiation on mouse hippocampus

Author

Casciati, Arianna, primary, Dobos, Katalin, additional, Antonelli, Francesca, additional, Benedek, Anett, additional, Kempf, Stefan J., additional, Bellés, Montserrat, additional, Balogh, Andrea, additional, Tanori, Mirella, additional, Heredia, Luis, additional, Atkinson, Michael J., additional, von Toerne, Christine, additional, Azimzadeh, Omid, additional, Saran, Anna, additional, Sáfrány, Geza, additional, Benotmane, Mohammed A., additional, Linares-Vidal, M. Victoria, additional, Tapio, Soile, additional, Lumniczky, Katalin, additional, and Pazzaglia, Simonetta, additional

Published
2016
Full Text
View/download PDF

35. A multidisciplinary approach unravels early and persistent effects of X-ray exposure at the onset of prenatal neurogenesis

Author

Verreet, Tine, primary, Quintens, Roel, additional, Van Dam, Debby, additional, Verslegers, Mieke, additional, Tanori, Mirella, additional, Casciati, Arianna, additional, Neefs, Mieke, additional, Leysen, Liselotte, additional, Michaux, Arlette, additional, Janssen, Ann, additional, D’Agostino, Emiliano, additional, Vande Velde, Greetje, additional, Baatout, Sarah, additional, Moons, Lieve, additional, Pazzaglia, Simonetta, additional, Saran, Anna, additional, Himmelreich, Uwe, additional, De Deyn, Peter Paul, additional, and Benotmane, Mohammed Abderrafi, additional

Published
2015
Full Text
View/download PDF

36. The cognitive defects of neonatally irradiated miceare accompanied by changed synaptic plasticity,adult neurogenesis and neuroinflammation

Author

Kempf, Stefan J., Casciati, Arianna, Buratovic, Sonja, Janik, Dirk, von Toerne, Christine, Ueffing, Marius, Neff, Frauke, Moertl, Simone, Stenerlöw, Bo, Saran, Anna, Atkinson, Michael J., Eriksson, Per, Pazzaglia, Simonetta, Tapio, Soile, Kempf, Stefan J., Casciati, Arianna, Buratovic, Sonja, Janik, Dirk, von Toerne, Christine, Ueffing, Marius, Neff, Frauke, Moertl, Simone, Stenerlöw, Bo, Saran, Anna, Atkinson, Michael J., Eriksson, Per, Pazzaglia, Simonetta, and Tapio, Soile

Abstract

Background/purpose of the study: Epidemiological evidence suggests that low doses of ionising radiation(≤1.0 Gy) produce persistent alterations in cognition if the exposure occurs at a young age. The mechanismsunderlying such alterations are unknown. We investigated the long-term effects of low doses of total body gammaradiation on neonatally exposed NMRI mice on the molecular and cellular level to elucidate neurodegeneration.Results: Significant alterations in spontaneous behaviour were observed at 2 and 4 months following a single 0.5or 1.0 Gy exposure. Alterations in the brain proteome, transcriptome, and several miRNAs were analysed 6–7months post-irradiation in the hippocampus, dentate gyrus (DG) and cortex. Signalling pathways related to synapticactin remodelling such as the Rac1-Cofilin pathway were altered in the cortex and hippocampus. Further, synapticproteins MAP-2 and PSD-95 were increased in the DG and hippocampus (1.0 Gy). The expression of synapticplasticity genes Arc, c-Fos and CREB was persistently reduced at 1.0 Gy in the hippocampus and cortex. Thesechanges were coupled to epigenetic modulation via increased levels of microRNAs (miR-132/miR-212, miR-134).Astrogliosis, activation of insulin-growth factor/insulin signalling and increased level of microglial cytokine TNFαindicated radiation-induced neuroinflammation. In addition, adult neurogenesis within the DG was persistentlynegatively affected after irradiation, particularly at 1.0 Gy.Conclusion: These data suggest that neurocognitive disorders may be induced in adults when exposed at a youngage to low and moderate cranial doses of radiation. This raises concerns about radiation safety standards andregulatory practices.

Published
2014
Full Text
View/download PDF

37. The cognitive defects of neonatally irradiated mice are accompanied by changed synaptic plasticity, adult neurogenesis and neuroinflammation

Author

Kempf, Stefan J, primary, Casciati, Arianna, additional, Buratovic, Sonja, additional, Janik, Dirk, additional, Toerne, Christine von, additional, Ueffing, Marius, additional, Neff, Frauke, additional, Moertl, Simone, additional, Stenerlöw, Bo, additional, Saran, Anna, additional, Atkinson, Michael J, additional, Eriksson, Per, additional, Pazzaglia, Simonetta, additional, and Tapio, Soile, additional

Published
2014
Full Text
View/download PDF

45. Astroglial Inhibition of NF-kB Does Not Ameliorate Disease Onset and Progression in a Mouse Model for Amyotrophic Lateral Sclerosis (ALS).

Author

Crosio, Claudia, Valle, Cristiana, Casciati, Arianna, Iaccarino, Ciro, and Carrì, Maria Teresa

Subjects
AMYOTROPHIC lateral sclerosis, ASTROCYTES, NEUROGLIA, NEURONS, INFLAMMATION, MOTOR neurons, TRANSGENIC mice
Abstract

Motor neuron death in amyotrophic lateral sclerosis (ALS) is considered a ''non-cell autonomous'' process, with astrocytes playing a critical role in disease progression. Glial cells are activated early in transgenic mice expressing mutant SOD1, suggesting that neuroinflammation has a relevant role in the cascade of events that trigger the death of motor neurons. An inflammatory cascade including COX2 expression, secretion of cytokines and release of NO from astrocytes may descend from activation of a NF-κB-mediated pathway observed in astrocytes from ALS patients and in experimental models. We have attempted rescue of transgenic mutant SOD1 mice through the inhibition of the NF-κB pathway selectively in astrocytes. Here we show that despite efficient inhibition of this major pathway, double transgenic mice expressing the mutant SOD1G93A ubiquitously and the dominant negative form of IkBa (IκBaAA) in astrocytes under control of the GFAP promoter show no benefit in terms of onset and progression of disease. Our data indicate that motor neuron death in ALS cannot be prevented by inhibition of a single inflammatory pathway because alternative pathways are activated in the presence of a persistent toxic stimulus. [ABSTRACT FROM AUTHOR]

Published
2011
Full Text
View/download PDF

46. SUMCASTEC_200621_NA_BIOEM 2020_Conference_Paper_pdf_Oxford_M.Tanori_Public_NA

Author

Tanori, Mirella, Casciati, Arianna, Zambotti, Alessandro, Pinto, Rosanna, Marino, Carmela, Mariateresa Mancuso, and Merla, Caterina

Subjects
stomatognathic diseases, neoplasms, nervous system diseases, 3. Good health
Abstract

Ultrashort pulsed electric fields as specific radiosensitizers of medulloblastoma cancer stem cells paper accepted to presentation to BIOEM2020 conference

47. SUMCASTEC_181003_NA_EMF-Med 2018 Pres_Conference presentation_.pdf_Bangor_C. Palego_Public_NA

Author

Gamal, Wesam, Pinto, Rosanna, Tanori, Mirella, Casciati, Arianna, Mancuso, Mariateresa, Persano, Luca, Viola, Giampietro, Saada, Sofiane, Bessette, Barbara, Lalloue, Fabrice, Pothier, Arnaud, Palego, Cristiano, and Merla, Caterina

Subjects
Permittivity, Cancer Stem Cells, Electromagnetic Stimulation, 4. Education
Abstract

Presentation of paper entitled "Permittivity measurements of brain cancer cell towards the electric characterisation of single Cancer Stem Cells" at the 1st EMF-Med World Conference on Biomedical Applications of Electromagnetic Fields. Presented by Dr C. Palego in Split, Croatia, September 10th 2018., Bangor University also gratefully acknowledges the financial support provided by the Welsh Government and Higher Education Funding Council for Wales through the Sêr Cymru National Research Network in Advanced Engineering and Materials.

48. SUMCASTEC_181003_NA_EMF-Med 2018 Pres_Conference presentation_.pdf_Bangor_C. Palego_Public_NA

Author

Gamal, Wesam, Pinto, Rosanna, Tanori, Mirella, Casciati, Arianna, Mancuso, Mariateresa, Persano, Luca, Viola, Giampietro, Saada, Sofiane, Bessette, Barbara, Lalloue, Fabrice, Pothier, Arnaud, Palego, Cristiano, and Merla, Caterina

Subjects
Permittivity, Cancer Stem Cells, Electromagnetic Stimulation, 4. Education
Abstract

Presentation of paper entitled "Permittivity measurements of brain cancer celltowards the electric characterisation ofsingle Cancer Stem Cells" at the 1st EMF-Med World Conference on Biomedical Applications of Electromagnetic Fields. Presented by Dr C. Palego inSplit, Croatia,September 10th 2018.

49. Synthetic lethal genetic interactions between Rad54 and PARP-1 in mouse development and oncogenesis

Author

Anna Saran, Simona Leonardi, Francesca Antonelli, Gabriele Babini, Emanuela Pasquali, Barbara Tanno, Ilaria De Stefano, Arianna Casciati, Mariateresa Mancuso, Alessandro Pannicelli, Paola Giardullo, Simonetta Pazzaglia, Francesco Berardinelli, Antonella Sgura, Mirella Tanori, Pazzaglia, S., Saran, A., Mancuso, M., Pannicelli, A., Tanno, B., Antonelli, F., Pasquali, E., Leonardi, S., Casciati, A., Tanori, M., Tanori, Mirella, Casciati, Arianna, Berardinelli, Francesco, Leonardi, Simona, Pasquali, Emanuela, Antonelli, Francesca, Tanno, Barbara, Pannicelli, Alessandro, Babini, Gabriele, De Stefano, Ilaria, Sgura, Antonella, Mancuso, Mariateresa, Saran, Anna, and Pazzaglia, Simonetta

Subjects
0301 basic medicine, Senescence, Expression profiles, DNA repair, DNA damage, Poly ADP ribose polymerase, Apoptosis, Biology, medicine.disease_cause, 03 medical and health sciences, Germline mutation, Expression profile, Cerebellum, medicine, Genetics, Medulloblastoma, fungi, Apoptosi, medicine.disease, 030104 developmental biology, Oncology, Cancer research, Homologous recombination, Carcinogenesis, Research Paper
Abstract

// Mirella Tanori 1 , Arianna Casciati 1 , Francesco Berardinelli 2 , Simona Leonardi 1 , Emanuela Pasquali 1 , Francesca Antonelli 1 , Barbara Tanno 1 , Paola Giardullo 2,3 , Alessandro Pannicelli 4 , Gabriele Babini 5 , Ilaria De Stefano 3 , Antonella Sgura 2 , Mariateresa Mancuso 1 , Anna Saran 1 and Simonetta Pazzaglia 1 1 Laboratory of Biomedical Technologies, Agenzia Nazionale per le Nuove Tecnologie, l’Energia e lo Sviluppo Economico Sostenibile (ENEA), CR-Casaccia, Rome, Italy 2 Department of Science, University Roma Tre, Rome, Italy 3 Department of Radiation Physics, Universita degli Studi Guglielmo Marconi, Rome, Italy 4 Technical Unit of Energetic Efficiency, ENEA, Rome, Italy 5 Department of Physics, University of Pavia, Pavia, Italy Correspondence to: Simonetta Pazzaglia, email: // Keywords : cerebellum, expression profiles, medulloblastoma, apoptosis, senescence Received : June 14, 2016 Accepted : June 26, 2016 Published : July 07, 2016 Abstract Mutations in DNA repair pathways are frequent in human cancers. Hence, gaining insights into the interaction of DNA repair genes is key to development of novel tumor-specific treatment strategies. In this study, we tested the functional relationship in development and oncogenesis between the homologous recombination (HR) factor Rad54 and Parp-1 , a nuclear enzyme that plays a multifunctional role in DNA damage signaling and repair. We introduced single or combined Rad54 and Parp-1 inactivating germline mutations in Ptc1 heterozygous mice, a well-characterized model of medulloblastoma, the most common malignant pediatric brain tumor. Our study reveals that combined inactivation of Rad54 and Parp-1 causes a marked growth delay culminating in perinatallethality, providing for the first time evidence of synthetic lethal interactions between Rad54 and Parp-1 in vivo . Although the double mutation hampered investigation of Rad54 and Parp-1 interactions in cerebellum tumorigenesis, insights were gained by showing accumulation of endogenous DNA damage and increased apoptotic rate in granule cell precursors (GCPs). A network-based approach to detect differential expression of DNA repair genes in the cerebellum revealed perturbation of p53 signaling in Rad54 -/- / Parp-1 -/- / Ptc1 +/- , and MEFs from combined Rad54/Parp-1 mutants showed p53/p21-dependent typical senescent features. These findings help elucidate the genetic interplay between Rad54 and Parp-1 by suggesting that p53/p21-mediated apoptosis and/or senescence may be involved in synthetic lethal interactions occurring during development and inhibition of tumor growth.

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results