508 results on '"Carpino G"'
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2. Correction: Circulating miR‑26b‑5p and miR‑451a as diagnostic biomarkers in medullary thyroid carcinoma patients
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Besharat, Z. M., Trocchianesi, S., Verrienti, A., Ciampi, R., Cantara, S., Romei, C., Sabato, C., Noviello, T. M. R., Po, A., Citarella, A., Caruso, F. P., Panariello, I., Gianno, F., Carpino, G., Gaudio, E., Chiacchiarini, M., Masuelli, L., Sponziello, M., Pecce, V., Ramone, T., Maino, F., Dotta, F., Ceccarelli, M., Pezzullo, L., Durante, C., Castagna, M. G., Elisei, R., and Ferretti, E.
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- 2024
- Full Text
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3. OC.06.2: POTENTIALOFA NATURAL COMPOUNDAS HEDGEHOG PATHWAY INHIBITOR FOR THE TREATMENT OF INTRAHEPATIC CHOLANGIOCARCINOMA
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Paradiso, S., primary, Carpino, G., additional, Quaglio, D., additional, Ghirga, F., additional, Di Meo, C., additional, Paoletti, L., additional, De Luca, T., additional, Franchitto, M., additional, Di Marcotullio, L., additional, Infante, P., additional, Gaudio, E., additional, Alvaro, D., additional, and Cardinale, V., additional
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- 2024
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4. Cell Therapy and Bioengineering in Experimental Liver Regenerative Medicine: In Vivo Injury Models and Grafting Strategies
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Amato, G., Saleh, T., Carpino, G., Gaudio, E., Alvaro, D., and Cardinale, V.
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- 2021
- Full Text
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5. Criteria for preclinical models of cholangiocarcinoma: scientific and medical relevance
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Calvisi, D, Boulter, L, Vaquero, J, Saborowski, A, Fabris, L, Rodrigues, P, Coulouarn, C, Castro, R, Segatto, O, Raggi, C, van der Laan, L, Carpino, G, Goeppert, B, Roessler, S, Kendall, T, Evert, M, Gonzalez-Sanchez, E, Valle, J, Vogel, A, Bridgewater, J, Borad, M, Gores, G, Roberts, L, Marin, J, Andersen, J, Alvaro, D, Forner, A, Banales, J, Cardinale, V, Macias, R, Vicent, S, Chen, X, Braconi, C, Verstegen, M, Fouassier, L, Scheiter, A, Selaru, F, Evert, K, Utpatel, K, Broutier, L, Cadamuro, M, Huch, M, Goldin, R, Gradilone, S, Saito, Y, Calvisi D. F., Boulter L., Vaquero J., Saborowski A., Fabris L., Rodrigues P. M., Coulouarn C., Castro R. E., Segatto O., Raggi C., van der Laan L. J. W., Carpino G., Goeppert B., Roessler S., Kendall T. J., Evert M., Gonzalez-Sanchez E., Valle J. W., Vogel A., Bridgewater J., Borad M. J., Gores G. J., Roberts L. R., Marin J. J. G., Andersen J. B., Alvaro D., Forner A., Banales J. M., Cardinale V., Macias R. I. R., Vicent S., Chen X., Braconi C., Verstegen M. M. A., Fouassier L., Roberts L., Scheiter A., Selaru F. M., Evert K., Utpatel K., Broutier L., Cadamuro M., Huch M., Goldin R., Gradilone S. A., Saito Y., Calvisi, D, Boulter, L, Vaquero, J, Saborowski, A, Fabris, L, Rodrigues, P, Coulouarn, C, Castro, R, Segatto, O, Raggi, C, van der Laan, L, Carpino, G, Goeppert, B, Roessler, S, Kendall, T, Evert, M, Gonzalez-Sanchez, E, Valle, J, Vogel, A, Bridgewater, J, Borad, M, Gores, G, Roberts, L, Marin, J, Andersen, J, Alvaro, D, Forner, A, Banales, J, Cardinale, V, Macias, R, Vicent, S, Chen, X, Braconi, C, Verstegen, M, Fouassier, L, Scheiter, A, Selaru, F, Evert, K, Utpatel, K, Broutier, L, Cadamuro, M, Huch, M, Goldin, R, Gradilone, S, Saito, Y, Calvisi D. F., Boulter L., Vaquero J., Saborowski A., Fabris L., Rodrigues P. M., Coulouarn C., Castro R. E., Segatto O., Raggi C., van der Laan L. J. W., Carpino G., Goeppert B., Roessler S., Kendall T. J., Evert M., Gonzalez-Sanchez E., Valle J. W., Vogel A., Bridgewater J., Borad M. J., Gores G. J., Roberts L. R., Marin J. J. G., Andersen J. B., Alvaro D., Forner A., Banales J. M., Cardinale V., Macias R. I. R., Vicent S., Chen X., Braconi C., Verstegen M. M. A., Fouassier L., Roberts L., Scheiter A., Selaru F. M., Evert K., Utpatel K., Broutier L., Cadamuro M., Huch M., Goldin R., Gradilone S. A., and Saito Y.
- Abstract
Cholangiocarcinoma (CCA) is a rare malignancy that develops at any point along the biliary tree. CCA has a poor prognosis, its clinical management remains challenging, and effective treatments are lacking. Therefore, preclinical research is of pivotal importance and necessary to acquire a deeper understanding of CCA and improve therapeutic outcomes. Preclinical research involves developing and managing complementary experimental models, from in vitro assays using primary cells or cell lines cultured in 2D or 3D to in vivo models with engrafted material, chemically induced CCA or genetically engineered models. All are valuable tools with well-defined advantages and limitations. The choice of a preclinical model is guided by the question(s) to be addressed; ideally, results should be recapitulated in independent approaches. In this Consensus Statement, a task force of 45 experts in CCA molecular and cellular biology and clinicians, including pathologists, from ten countries provides recommendations on the minimal criteria for preclinical models to provide a uniform approach. These recommendations are based on two rounds of questionnaires completed by 35 (first round) and 45 (second round) experts to reach a consensus with 13 statements. An agreement was defined when at least 90% of the participants voting anonymously agreed with a statement. The ultimate goal was to transfer basic laboratory research to the clinics through increased disease understanding and to develop clinical biomarkers and innovative therapies for patients with CCA.
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- 2023
6. Reply to: “Ductular reaction is a prognostic factor in primary biliary cholangitis”
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Carpino, G, Cardinale, V, Carbone, M, Carpino G., Cardinale V., Carbone M., Carpino, G, Cardinale, V, Carbone, M, Carpino G., Cardinale V., and Carbone M.
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- 2023
7. Correction: Circulating miR‑26b‑5p and miR‑451a as diagnostic biomarkers in medullary thyroid carcinoma patients
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Besharat, Z. M., primary, Trocchianesi, S., additional, Verrienti, A., additional, Ciampi, R., additional, Cantara, S., additional, Romei, C., additional, Sabato, C., additional, Noviello, T. M. R., additional, Po, A., additional, Citarella, A., additional, Caruso, F. P., additional, Panariello, I., additional, Gianno, F., additional, Carpino, G., additional, Gaudio, E., additional, Chiacchiarini, M., additional, Masuelli, L., additional, Sponziello, M., additional, Pecce, V., additional, Ramone, T., additional, Maino, F., additional, Dotta, F., additional, Ceccarelli, M., additional, Pezzullo, L., additional, Durante, C., additional, Castagna, M. G., additional, Elisei, R., additional, and Ferretti, E., additional
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- 2023
- Full Text
- View/download PDF
8. Role of ductular reaction and ductular–canalicular junctions in identifying severe primary biliary cholangitis
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Overi, D, Carpino, G, Cristoferi, L, Onori, P, Kennedy, L, Francis, H, Zucchini, N, Rigamonti, C, Vigano, M, Floreani, A, D'Amato, D, Gerussi, A, Venere, R, Alpini, G, Glaser, S, Alvaro, D, Invernizzi, P, Gaudio, E, Cardinale, V, Carbone, M, Overi D., Carpino G., Cristoferi L., Onori P., Kennedy L., Francis H., Zucchini N., Rigamonti C., Vigano M., Floreani A., D'Amato D., Gerussi A., Venere R., Alpini G., Glaser S., Alvaro D., Invernizzi P., Gaudio E., Cardinale V., Carbone M., Overi, D, Carpino, G, Cristoferi, L, Onori, P, Kennedy, L, Francis, H, Zucchini, N, Rigamonti, C, Vigano, M, Floreani, A, D'Amato, D, Gerussi, A, Venere, R, Alpini, G, Glaser, S, Alvaro, D, Invernizzi, P, Gaudio, E, Cardinale, V, Carbone, M, Overi D., Carpino G., Cristoferi L., Onori P., Kennedy L., Francis H., Zucchini N., Rigamonti C., Vigano M., Floreani A., D'Amato D., Gerussi A., Venere R., Alpini G., Glaser S., Alvaro D., Invernizzi P., Gaudio E., Cardinale V., and Carbone M.
- Abstract
Background & Aims: Primary biliary cholangitis (PBC) is a chronic cholangiopathy characterised by immuno-mediated injury of interlobular bile ducts leading to intrahepatic cholestasis and progressive liver fibrosis. PBC histology is characterised by portal inflammation, progressive fibrosis, ductopenia, and the appearance of the so-called ductular reaction. The aim of the present study was to investigate the pathogenetic relevance of ductular reaction in PBC. Methods: Liver biopsies were collected from naïve people with PBC (N = 87). Clinical–serological parameters were obtained at diagnosis and after 1 year of ursodeoxycholic acid (UDCA) treatment. Histological staging was performed on all slides according to multiple scoring systems and criteria for PBC. Liver samples were obtained from Mdr2−/− mice treated with or without UDCA. Samples were processed for histology, immunohistochemistry, and immunofluorescence. Results: Ductular reaction in people with PBC correlated with the disease stage and liver fibrosis, but not with disease activity; an extensive ductular reaction correlated with serum alkaline phosphatase levels at diagnosis, response to UDCA, and individuals’ estimated survival, independently from other histological parameters, including disease stage. In people with PBC, reactive ductules were associated with the establishment of junctions with bile canaliculi and with fibrogenetic cell activation. Consistently, in a mouse model of intrahepatic cholestasis, UDCA treatment was effective in reducing ductular reaction and fibrosis and increasing ductular–canalicular junctions. Conclusions: Extensive ductular reaction outlines a severe histologic phenotype in PBC and is associated with an inadequate therapy response and a worse estimated prognosis. Lay summary: In people affected by primary biliary cholangitis (PBC), the histological appearance of extensive ductular reaction identifies individuals at risk of progressive fibrosis. Ductular reaction at dia
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- 2022
9. The Italian law on body donation: A position paper of the Italian College of Anatomists
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De Caro, R, Boscolo-Berto, R, Artico, M, Bertelli, E, Cannas, M, Cappello, F, Carpino, G, Castorina, S, Cataldi, A, Cavaletti, G, Cinti, S, Cocco, L, Cremona, O, Crivellato, E, De Luca, A, Falconi, M, Familiari, G, Ferri, G, Fornai, F, Gesi, M, Geuna, S, Gibelli, D, Giordano, A, Gobbi, P, Guerra, G, Gulisano, M, Macchi, V, Macchiarelli, G, Manzoli, L, Michetti, F, Miscia, S, Montagnani, S, Montella, A, Morini, S, Onori, P, Palumbo, C, Papa, M, Porzionato, A, Quacci, D, Raspanti, M, Rende, M, Rezzani, R, Ribatti, D, Ripani, M, Rodella, L, Rossi, P, Sbarbati, A, Secchiero, P, Sforza, C, Stecco, C, Toni, R, Vercelli, A, Vitale, M, Zancanaro, C, Zauli, G, Zecchi, S, Anastasi, G, Gaudio, E, De Caro R., Boscolo-Berto R., Artico M., Bertelli E., Cannas M., Cappello F., Carpino G., Castorina S., Cataldi A., Cavaletti G. A., Cinti S., Cocco L. I., Cremona O., Crivellato E., De Luca A., Falconi M., Familiari G., Ferri G. L., Fornai F., Gesi M., Geuna S., Gibelli D. M., Giordano A., Gobbi P., Guerra G., Gulisano M., Macchi V., Macchiarelli G., Manzoli L., Michetti F., Miscia S., Montagnani S., Montella A. C. M., Morini S., Onori P., Palumbo C., Papa M., Porzionato A., Quacci D. E., Raspanti M., Rende M., Rezzani R., Ribatti D., Ripani M., Rodella L. F., Rossi P., Sbarbati A., Secchiero P., Sforza C., Stecco C., Toni R., Vercelli A., Vitale M., Zancanaro C., Zauli G., Zecchi S., Anastasi G. P., Gaudio E., De Caro, R, Boscolo-Berto, R, Artico, M, Bertelli, E, Cannas, M, Cappello, F, Carpino, G, Castorina, S, Cataldi, A, Cavaletti, G, Cinti, S, Cocco, L, Cremona, O, Crivellato, E, De Luca, A, Falconi, M, Familiari, G, Ferri, G, Fornai, F, Gesi, M, Geuna, S, Gibelli, D, Giordano, A, Gobbi, P, Guerra, G, Gulisano, M, Macchi, V, Macchiarelli, G, Manzoli, L, Michetti, F, Miscia, S, Montagnani, S, Montella, A, Morini, S, Onori, P, Palumbo, C, Papa, M, Porzionato, A, Quacci, D, Raspanti, M, Rende, M, Rezzani, R, Ribatti, D, Ripani, M, Rodella, L, Rossi, P, Sbarbati, A, Secchiero, P, Sforza, C, Stecco, C, Toni, R, Vercelli, A, Vitale, M, Zancanaro, C, Zauli, G, Zecchi, S, Anastasi, G, Gaudio, E, De Caro R., Boscolo-Berto R., Artico M., Bertelli E., Cannas M., Cappello F., Carpino G., Castorina S., Cataldi A., Cavaletti G. A., Cinti S., Cocco L. I., Cremona O., Crivellato E., De Luca A., Falconi M., Familiari G., Ferri G. L., Fornai F., Gesi M., Geuna S., Gibelli D. M., Giordano A., Gobbi P., Guerra G., Gulisano M., Macchi V., Macchiarelli G., Manzoli L., Michetti F., Miscia S., Montagnani S., Montella A. C. M., Morini S., Onori P., Palumbo C., Papa M., Porzionato A., Quacci D. E., Raspanti M., Rende M., Rezzani R., Ribatti D., Ripani M., Rodella L. F., Rossi P., Sbarbati A., Secchiero P., Sforza C., Stecco C., Toni R., Vercelli A., Vitale M., Zancanaro C., Zauli G., Zecchi S., Anastasi G. P., and Gaudio E.
- Abstract
In Italy, recent legislation (Law No. 10/2020) has tuned regulations concerning the donation of one's postmortem body and tissues for study, training, and scientific research purposes. This study discusses several specific issues to optimise the applicability and effectiveness of such an important, novel regulatory setting. Critical issues arise concerning the learners, the type of training and teaching activities that can be planned, the position of academic anatomy institutes, the role of family members in the donation process, the time frame of the donation process, the eligibility of partial donation, or the simultaneous donation of organs and tissues to patients awaiting transplantation. In particular, a universal time limit for donations (i.e., one year) makes it impossible to plan the long-term use of specific body parts, which could be effectively preserved for the advanced teaching and training of medical students and surgeons. The abovementioned conditions lead to the limited use of corpses, thus resulting in the inefficiency of the whole system of body donation. Overall, the donors’ scope for the donation of their body could be best honoured by a more flexible and tuneable approach that can be used on a case-by-case basis. Furthermore, it is deemed necessary to closely monitor the events scheduled for corpses in public nonacademic institutions or private enterprises. This paper presents useful insights from Italian anatomists with the hope of providing inspiration for drafting the regulations. In conclusion, this paper focuses on the critical issues derived from the recently introduced Italian law on the donation and use of the body after death and provides suggestions to lawmakers for future implementations.
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- 2021
10. Assessment of bile duct injury of donor livers during ex situ normothermic machine perfusion
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de Jong, I. E. M., Bodewes, S. B., Overi, D., van Leeuwen, O. B., van den Heuvel, M. C., Carpino, G., Gaudio, E., de Meijer, V. E., Porte, R. J., Groningen Institute for Organ Transplantation (GIOT), and Center for Liver, Digestive and Metabolic Diseases (CLDM)
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- 2022
11. Cholangiocarcinoma 2020: the next horizon in mechanisms and management
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Banales, J, Marin, J, Lamarca, A, Rodrigues, P, Khan, S, Roberts, L, Cardinale, V, Carpino, G, Andersen, J, Braconi, C, Calvisi, D, Perugorria, M, Fabris, L, Boulter, L, Macias, R, Gaudio, E, Alvaro, D, Gradilone, S, Strazzabosco, M, Marzioni, M, Coulouarn, C, Fouassier, L, Raggi, C, Invernizzi, P, Mertens, J, Moncsek, A, Rizvi, S, Heimbach, J, Koerkamp, B, Bruix, J, Forner, A, Bridgewater, J, Valle, J, Gores, G, Banales JM, Marin JJG, Lamarca A, Rodrigues PM, Khan SA, Roberts LR, Cardinale V, Carpino G, Andersen JB, Braconi C, Calvisi DF, Perugorria MJ, Fabris L, Boulter L, Macias RIR, Gaudio E, Alvaro D, Gradilone SA, Strazzabosco M, Marzioni M, Coulouarn C, Fouassier L, Raggi C, Invernizzi P, Mertens JC, Moncsek A, Rizvi S, Heimbach J, Koerkamp BG, Bruix J, Forner A, Bridgewater J, Valle JW, Gores GJ, Banales, J, Marin, J, Lamarca, A, Rodrigues, P, Khan, S, Roberts, L, Cardinale, V, Carpino, G, Andersen, J, Braconi, C, Calvisi, D, Perugorria, M, Fabris, L, Boulter, L, Macias, R, Gaudio, E, Alvaro, D, Gradilone, S, Strazzabosco, M, Marzioni, M, Coulouarn, C, Fouassier, L, Raggi, C, Invernizzi, P, Mertens, J, Moncsek, A, Rizvi, S, Heimbach, J, Koerkamp, B, Bruix, J, Forner, A, Bridgewater, J, Valle, J, Gores, G, Banales JM, Marin JJG, Lamarca A, Rodrigues PM, Khan SA, Roberts LR, Cardinale V, Carpino G, Andersen JB, Braconi C, Calvisi DF, Perugorria MJ, Fabris L, Boulter L, Macias RIR, Gaudio E, Alvaro D, Gradilone SA, Strazzabosco M, Marzioni M, Coulouarn C, Fouassier L, Raggi C, Invernizzi P, Mertens JC, Moncsek A, Rizvi S, Heimbach J, Koerkamp BG, Bruix J, Forner A, Bridgewater J, Valle JW, and Gores GJ
- Abstract
Cholangiocarcinoma (CCA) includes a cluster of highly heterogeneous biliary malignant tumours that can arise at any point of the biliary tree. Their incidence is increasing globally, currently accounting for ~15% of all primary liver cancers and ~3% of gastrointestinal malignancies. The silent presentation of these tumours combined with their highly aggressive nature and refractoriness to chemotherapy contribute to their alarming mortality, representing ~2% of all cancer-related deaths worldwide yearly. The current diagnosis of CCA by non-invasive approaches is not accurate enough, and histological confirmation is necessary. Furthermore, the high heterogeneity of CCAs at the genomic, epigenetic and molecular levels severely compromises the efficacy of the available therapies. In the past decade, increasing efforts have been made to understand the complexity of these tumours and to develop new diagnostic tools and therapies that might help to improve patient outcomes. In this expert Consensus Statement, which is endorsed by the European Network for the Study of Cholangiocarcinoma, we aim to summarize and critically discuss the latest advances in CCA, mostly focusing on classification, cells of origin, genetic and epigenetic abnormalities, molecular alterations, biomarker discovery and treatments. Furthermore, the horizon of CCA for the next decade from 2020 onwards is highlighted.
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- 2020
12. Accuracy of Liver Stiffness Measurement in assessing liver fibrosis in naïve patients with Primary Biliary Cholangitis
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Cristoferi, L, Nardi, A, Viganò, M, Rigamonti, C, Degasperi, E, Cardinale, V, Labanca, S, Zucchini, N, Leutner, M, Venere, R, Picciotto, A, Cazzagon, N, Lucà, M, Overi, D, Gerussi, A, D’Amato, D, O’Donnell, S, Cerini, F, De Benedittis, C, Cadamuro, M, Malinverno, F, Floreani, A, Alvaro, D, Gaudio, E, Invernizzi, P, Carpino, G, Carbone, M, Cristoferi L, Nardi A, Viganò M, Rigamonti C, Degasperi E, Cardinale V, Labanca S, Zucchini N, Leutner M, Venere R, Picciotto A, Cazzagon N, Lucà M, Overi D, Gerussi A, D’Amato D, O’Donnell S, Cerini F, De Benedittis C, Cadamuro M, Malinverno F, Floreani A, Alvaro D, Gaudio E, Invernizzi P, Carpino G, Carbone M, Cristoferi, L, Nardi, A, Viganò, M, Rigamonti, C, Degasperi, E, Cardinale, V, Labanca, S, Zucchini, N, Leutner, M, Venere, R, Picciotto, A, Cazzagon, N, Lucà, M, Overi, D, Gerussi, A, D’Amato, D, O’Donnell, S, Cerini, F, De Benedittis, C, Cadamuro, M, Malinverno, F, Floreani, A, Alvaro, D, Gaudio, E, Invernizzi, P, Carpino, G, Carbone, M, Cristoferi L, Nardi A, Viganò M, Rigamonti C, Degasperi E, Cardinale V, Labanca S, Zucchini N, Leutner M, Venere R, Picciotto A, Cazzagon N, Lucà M, Overi D, Gerussi A, D’Amato D, O’Donnell S, Cerini F, De Benedittis C, Cadamuro M, Malinverno F, Floreani A, Alvaro D, Gaudio E, Invernizzi P, Carpino G, and Carbone M
- Abstract
Background and Aims: Non-invasive evaluation of liver fibrosis in primary biliary cholangitis (PBC) with Liver Stiffness Measurements (LSM) by Transient Elastography (TE) is routinely undertaken at diagnosis for disease staging and risk stratification. However, evidence on correlation between LSM and liver fibrosis is based on cross-sectional studies in PBC-treated patients. Moreover, the impact of potential confounders, e.g. cholestasis and steatosis, is unclear. Aim of our study was to investigate the accuracy of LSM to predict moderate to severe fibrosis in newly diagnosed PBC patients naïve to therapy. Method: We collected data from 182 adult patients who underwent liver biopsy (LB) for PBC at diagnosis in five Italian liver centers from Jan 2006 through Aug 2019. TE examinations within 3 months from LB were included. LB were scored centrally by two expert pathologists, blinded to clinical data and disease stage, according to Ludwig staging system. In all patients Fibrosis-4 (FIB-4) and aspartate aminotransferase [AST]/platelet ratio (APRI) score have been calculated. Diagnostic accuracy of LSM, FIB-4 and APRI score was estimated using the area under the receiver operating characteristic curves (AUROCs) for fibrosis. The effects of liver biochemistry and histological parameters were appraised using multivariable logistic model. Results: 123 PBC patients had adequate LB (≥9 portal tracts) and valid LSM values. According to histological assessment, Ludwig stage distribution was as follows: stage I = 38 (30.9%), stage II = 46(37.4%), stage III = 27 (21.9%), stage IV = 12 (9.8%). TE identified patients with Ludwig stage III/IV with an AUROC of 0.83 (95% confidence interval [CI] (0.74, 0.90)) (Fig.1). The optimal threshold was identified at 6.75kPa (CI (6.55, 7.50)), with 85% of sensitivity, 75% of specificity, 78% of accuracy and 6 false negative. TE was superior to APRI and FIB-4 having AUROC of 0.638 (CI = (0.535, 0.740)) and 0.641 (CI = (0.516, 0.766)), respecti
- Published
- 2020
13. Accuracy of Transient Elastography in assessing fibrosis at diagnosis in naïve patients with Primary Biliary Cholangitis: a dual cut-off approach
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Cristoferi, L, Calvaruso, V, Overi, D, Viganò, M, Rigamonti, C, Degasperi, E, Cardinale, V, Labanca, S, Zucchini, N, Fichera, A, Di Marco, V, Leutner, M, Venere, R, Picciotto, A, Lucà, M, Mulinacci, G, Palermo, A, Gerussi, A, D'Amato, D, O'Donnell, S, Cerini, F, De Benedittis, C, Malinverno, F, Ronca, V, Mancuso, C, Cazzagon, N, Ciaccio, A, Barisani, D, Marzioni, M, Floreani, A, Alvaro, D, Gaudio, E, Invernizzi, P, Carpino, G, Nardi, A, Carbone, M, Cristoferi, Laura, Calvaruso, Vincenza, Overi, Diletta, Viganò, Mauro, Rigamonti, Cristina, Degasperi, Elisabetta, Cardinale, Vincenzo, Labanca, Sara, Zucchini, Nicola, Fichera, Anna, Di Marco, Vito, Leutner, Monica, Venere, Rosanna, Picciotto, Antonino, Lucà, Martina, Mulinacci, Giacomo, Palermo, Andrea, Gerussi, Alessio, D'Amato, Daphne, O'Donnell, Sarah Elisabeth, Cerini, Federica, De Benedittis, Carla, Malinverno, Federica, Ronca, Vincenzo, Mancuso, Clara, Cazzagon, Nora, Ciaccio, Antonio, Barisani, Donatella, Marzioni, Marco, Floreani, Annarosa, Alvaro, Domenico, Gaudio, Eugenio, Invernizzi, Pietro, Carpino, Guido, Nardi, Alessandra, Carbone, Marco, Cristoferi, L, Calvaruso, V, Overi, D, Viganò, M, Rigamonti, C, Degasperi, E, Cardinale, V, Labanca, S, Zucchini, N, Fichera, A, Di Marco, V, Leutner, M, Venere, R, Picciotto, A, Lucà, M, Mulinacci, G, Palermo, A, Gerussi, A, D'Amato, D, O'Donnell, S, Cerini, F, De Benedittis, C, Malinverno, F, Ronca, V, Mancuso, C, Cazzagon, N, Ciaccio, A, Barisani, D, Marzioni, M, Floreani, A, Alvaro, D, Gaudio, E, Invernizzi, P, Carpino, G, Nardi, A, Carbone, M, Cristoferi, Laura, Calvaruso, Vincenza, Overi, Diletta, Viganò, Mauro, Rigamonti, Cristina, Degasperi, Elisabetta, Cardinale, Vincenzo, Labanca, Sara, Zucchini, Nicola, Fichera, Anna, Di Marco, Vito, Leutner, Monica, Venere, Rosanna, Picciotto, Antonino, Lucà, Martina, Mulinacci, Giacomo, Palermo, Andrea, Gerussi, Alessio, D'Amato, Daphne, O'Donnell, Sarah Elisabeth, Cerini, Federica, De Benedittis, Carla, Malinverno, Federica, Ronca, Vincenzo, Mancuso, Clara, Cazzagon, Nora, Ciaccio, Antonio, Barisani, Donatella, Marzioni, Marco, Floreani, Annarosa, Alvaro, Domenico, Gaudio, Eugenio, Invernizzi, Pietro, Carpino, Guido, Nardi, Alessandra, and Carbone, Marco
- Abstract
Background and Aims: Liver fibrosis holds a relevant prognostic meaning in primary biliary cholangitis (PBC). Noninvasive fibrosis evaluation using vibration-controlled transient elastography (VCTE) is routinely performed. However, there is limited evidence on its accuracy at diagnosis in PBC. We aimed to estimate the diagnostic accuracy of VCTE in assessing advanced fibrosis (AF) at disease presentation in PBC. Approach and Results: We collected data from 167 consecutive treatment-naïve PBC patients who underwent liver biopsy (LB) at diagnosis at six Italian centers. VCTE examinations were completed within 12 weeks of LB. Biopsies were scored by two blinded expert pathologists, according to the Ludwig system. Diagnostic accuracy was estimated using the area under the receiver operating characteristic curves (AUROCs) for AF (Ludwig stage ≥III). Effects of biochemical and clinical parameters on liver stiffness measurement (LSM) were appraised. The derivation cohort consisted of 126 patients with valid LSM and LB; VCTE identified patients with AF with an AUROC of 0.89. LSM cutoffs ≤6.5 and >11.0 kPa enabled to exclude and confirm, respectively, AF (negative predictive value [NPV] = 0.94; positive predictive value [PPV] = 0.89; error rate = 5.6%). These values were externally validated in an independent cohort of 91 PBC patients (NPV = 0.93; PPV = 0.89; error rate = 8.6%). Multivariable analysis found that the only parameter affecting LSM was fibrosis stage. No association was found with BMI and liver biochemistry. Conclusions: In a multicenter study of treatment-naïve PBC patients, we identified two cutoffs (LSM ≤6.5 and >11.0 kPa) able to discriminate at diagnosis the absence or presence, respectively, of AF in PBC patients, with external validation. In patients with LSM between these two cutoffs, VCTE is not reliable and liver biopsy should be evaluated for accurate disease staging. BMI and liver biochemistry did not affect LSMs.
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- 2021
14. DCLK1, a putative novel stem cell marker in human cholangiocarcinoma
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Nevi, L, Di Matteo, S, Carpino, G, Zizzari, I, Safarikia, S, Ambrosino, V, Costantini, D, Overi, D, Giancotti, A, Monti, M, Bosco, D, De Peppo, V, Oddi, A, De Rose, A, Melandro, F, Bragazzi, M, Faccioli, J, Massironi, S, Grazi, G, Panici, P, Berloco, P, Giuliante, F, Cardinale, V, Invernizzi, P, Caretti, G, Gaudio, E, Alvaro, D, Nevi, Lorenzo, Di Matteo, Sabina, Carpino, Guido, Zizzari, Ilaria, Safarikia, Samira, Ambrosino, Valeria, Costantini, Daniele, Overi, Diletta, Giancotti, Antonella, Monti, Marco, Bosco, Daniela, De Peppo, Valerio, Oddi, Andrea, De Rose, Agostino Maria, Melandro, Fabio, Bragazzi, Maria Consiglia, Faccioli, Jessica, Massironi, Sara, Grazi, Gian Luca, Panici, Pierluigi Benedetti, Berloco, Paquale Bartomeo, Giuliante, Felice, Cardinale, Vincenzo, Invernizzi, Pietro, Caretti, Giuseppina, Gaudio, Eugenio, Alvaro, Domenico, Nevi, L, Di Matteo, S, Carpino, G, Zizzari, I, Safarikia, S, Ambrosino, V, Costantini, D, Overi, D, Giancotti, A, Monti, M, Bosco, D, De Peppo, V, Oddi, A, De Rose, A, Melandro, F, Bragazzi, M, Faccioli, J, Massironi, S, Grazi, G, Panici, P, Berloco, P, Giuliante, F, Cardinale, V, Invernizzi, P, Caretti, G, Gaudio, E, Alvaro, D, Nevi, Lorenzo, Di Matteo, Sabina, Carpino, Guido, Zizzari, Ilaria, Safarikia, Samira, Ambrosino, Valeria, Costantini, Daniele, Overi, Diletta, Giancotti, Antonella, Monti, Marco, Bosco, Daniela, De Peppo, Valerio, Oddi, Andrea, De Rose, Agostino Maria, Melandro, Fabio, Bragazzi, Maria Consiglia, Faccioli, Jessica, Massironi, Sara, Grazi, Gian Luca, Panici, Pierluigi Benedetti, Berloco, Paquale Bartomeo, Giuliante, Felice, Cardinale, Vincenzo, Invernizzi, Pietro, Caretti, Giuseppina, Gaudio, Eugenio, and Alvaro, Domenico
- Abstract
Background & aims: Cholangiocarcinoma (CCA) is a very aggressive cancer showing high cancer stem cells (CSCs) presence. Doublecortin-like kinase1 (DCLK1) has been demonstrated as a CSC marker in different gastroenterological solid tumours. Our aim was to evaluate in vitro the expression and the biological function of DCLK1 in intrahepatic CCA (iCCA) and perihilar CCA (pCCA). Approach & results: Specimens surgically resected of human CCA were enzymatically digested, submitted to immunosorting for specific CSC markers (LGR5, CD90, EpCAM, CD133, CD13) and primary cell cultures were prepared. DCLK1 expression was analysed in CCA cell cultures by real-time quantitative polymerase chain reaction (RT-qPCR), Western Blot and immunofluorescence. Functional studies have been performed by evaluating the effects of selective DCLK1 inhibitor (LRRK2-IN-1) on cell proliferation (MTS-Assay, cell population doubling time), apoptosis and colony formation capacity. DCLK1 was investigated in situ by immunohistochemistry and RT-qPCR. DCLK1 serum concentration was analysed by enzyme-linked immunosorbent assay (ELISA). We describe DCLK1 in CCA with an increased gene and protein DCLK1 expression in pCCALGR5+ and in iCCACD133+ cells compared to unsorted cells. LRRK2-IN-1 showed an anti-proliferative effect in dose-dependent manner. LRRK2-IN-1 markedly impaired cell proliferation, induced apoptosis, decreased colony formation capacity and colony size in both iCCA and pCCA compared to untreated cells. In situ analysis confirm that DCLK1 is present only in tumours, but not in healthy tissue. Interestingly, DCLK1 was detected in the human serum samples of iCCA (high), pCCA (high), HCC (low) and cirrhotic (low) patients, but it was almost undetectable in healthy controls. Conclusion: DCLK1 characterizes a specific CSC-subpopulation of iCCACD133+ and pCCALGR5+ and its inhibition exerts anti-neoplastic effects in primary CCA cell cultures. Human DCLK1 serum might represent a serum biomarke
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- 2021
15. Oncogenic signaling pathways in the Cancer Genome Atlas
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Sanchez-Vega, F., Mina, M., Armenia, J., Chatila, W. K., Luna, A., La, K. C., Dimitriadoy, S., Liu, D. L., Kantheti, H. S., Saghafinia, S., Chakravarty, D., Daian, F., Gao, Q., Bailey, M. H., Liang, W. -W., Foltz, S. M., Shmulevich, I., Ding, L., Heins, Z., Ochoa, A., Gross, B., Gao, J., Zhang, H., Kundra, R., Kandoth, C., Bahceci, I., Dervishi, L., Doğrusöz, Uğur, Zhou, W., Shen, H., Laird, P. W., Way, G. P., Greene, C. S., Liang, H., Xiao, Y., Wang, C., Iavarone, A., Berger, A. H., Bivona, T. G., Lazar, A. J., Hammer, G. D., Giordano, T., Kwong, L. N., McArthur, G., Huang, C., Tward, A. D., Frederick, M. J., McCormick, F., Meyerson, M., Caesar-Johnson, S. J., Demchok, J. A., Felau, I., Kasapi, M., Ferguson, M. L., Hutter, C. M., Sofia, H. J., Tarnuzzer, R., Wang, Z., Yang, L., Zenklusen, J. C., Zhang, J. J., Chudamani, S., Liu, J., Lolla, L., Naresh, R., Pihl, T., Sun, Q., Wan, Y., Wu, Y., Cho, J., DeFreitas, T., Frazer, S., Gehlenborg, N., Getz, G., Heiman, D. I., Kim, J., Lawrence, M. S., Lin, P., Meier, S., Noble, M. S., Saksena, G., Voet, D., Bernard, B., Chambwe, N., Dhankani, V., Knijnenburg, T., Kramer, R., Leinonen, K., Liu, Y., Miller, M., Reynolds, S., Thorsson, V., Zhang, W., Akbani, R., Broom, B. M., Hegde, A. M., Ju, Z., Kanchi, R. S., Korkut, A., Li, J., Ling, S., Liu W., Lu, Y., Mills, G. B., Ng, K. -S., Rao, A., Ryan, M., Wang, J., Weinstein, J. N., Zhang, J., Abeshouse, A., de, Bruijn, I., Gross, B. E., Heins, Z. J., La, K., Ladanyi, M., Nissan, M. G., Phillips, S. M., Reznik, E., Sander, C., Schultz, N., Sheridan, R., Sumer, S. O., Sun, Y., Taylor, B. S., Anur, P., Peto, M., Spellman, P., Benz, C., Stuart, J. M., Wong, C. K., Yau, C., Hayes, D. N., Parker, J. S., Wilkerson, M. D., Ally, A., Balasundaram, M., Bowlby, R., Brooks, D., Carlsen, R., Chuah, E., Dhalla, N., Holt, R., Jones, S. J. M., Kasaian, K., Lee, D., Ma, Y., Marra, M. A., Mayo, M., Moore, R. A., Mungall, A. J., Mungall, K., Robertson, A. G., Sadeghi, S., Schein, J. E., Sipahimalani, P., Tam, A., Thiessen, N., Tse, K., Wong, T., Berger, A. C., Beroukhim, R., Cherniack, A. D., Cibulskis, C., Gabriel, S. B., Gao, G. F., Ha, G., Schumacher, S. E., Shih, J., Kucherlapati, M. H., Kucherlapati, R. S., Baylin, S., Cope, L., Danilova, L., Bootwalla, M. S., Lai, P. H., Maglinte, D. T., Van, Den, Berg, D. J., Weisenberger, D. J., Auman, J. T., Balu, S., Bodenheimer, T., Fan, C., Hoadley, K. A., Hoyle, A. P., Jefferys, S. R., Jones, C. D., Meng, S., Mieczkowski, P. A., Mose, L. E., Perou, A. H., Perou, C. M., Roach, J., Shi, Y., Simons, J. V., Skelly, T., Soloway, M. G., Tan, D., Veluvolu, U., Fan, H., Hinoue, T., Bellair, M., Chang, K., Covington, K., Creighton, C. J., Dinh, H., Doddapaneni, H., Donehower, L. A., Drummond, J., Gibbs, R. 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Walker J., Zuna R., Feldman M., Valdivieso F., Dhir R., Luketich J., Pinero E.M.M., Quintero-Aguilo M., Carlotti C.G., Dos Santos J.S., Kemp R., Sankarankuty A., Tirapelli D., Catto J., Agnew K., Swisher E., Creaney J., Robinson B., Shelley C.S., Godwin E.M., Kendall S., Shipman C., Bradford C., Carey T., Haddad A., Moyer J., Peterson L., Prince M., Rozek L., Wolf G., Bowman R., Fong K.M., Yang I., Korst R., Rathmell W.K., Fantacone-Campbell J.L., Hooke J.A., Kovatich A.J., Shriver C.D., DiPersio J., Drake B., Govindan R., Heath S., Ley T., Van Tine B., Westervelt P., Rubin M.A., Lee J.I., Aredes N.D., Mariamidze A., Van Allen E.M., and Ciriello G.
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0301 basic medicine ,cancer genome atlas ,cancer genomics ,combination therapy ,pan-cancer ,PanCanAtlas ,precision oncology ,signaling pathways ,TCGA ,therapeutics ,whole exome sequencing ,Signaling pathways ,Somatic cell ,Wnt Protein ,Cancer Genome Atlas Research Network ,Biochemistry ,Medical and Health Sciences ,Phosphatidylinositol 3-Kinases ,Transforming Growth Factor beta ,Neoplasms ,Databases, Genetic ,LS2_1 ,Cancer genomics ,LS4_6 ,610 Medicine & health ,11 Medical and Health Sciences ,Cancer ,biology ,Wnt signaling pathway ,cancer genomic ,Precision oncology ,Biological Sciences ,Cell cycle ,DNA methylation ,Signal transduction ,CICLO CELULAR ,Life Sciences & Biomedicine ,Genes, Neoplasm ,Humans ,Neoplasms/genetics ,Neoplasms/pathology ,Phosphatidylinositol 3-Kinases/genetics ,Phosphatidylinositol 3-Kinases/metabolism ,Signal Transduction/genetics ,Transforming Growth Factor beta/genetics ,Transforming Growth Factor beta/metabolism ,Tumor Suppressor Protein p53/genetics ,Tumor Suppressor Protein p53/metabolism ,Wnt Proteins/genetics ,Wnt Proteins/metabolism ,Biotechnology ,Human ,Signal Transduction ,signaling pathway ,EXPRESSION ,Biochemistry & Molecular Biology ,GENES ,Pan-cancer ,Therapeutics ,General Biochemistry, Genetics and Molecular Biology ,NO ,Databases ,03 medical and health sciences ,Genetic ,Genetics ,Combination therapy ,Protein kinase B ,Gene ,SIGNATURES ,Cancer genome atlas ,Science & Technology ,LANDSCAPE ,MUTATIONS ,Biochemistry, Genetics and Molecular Biology(all) ,Human Genome ,Whole exome sequencing ,Cell Biology ,Transforming growth factor beta ,cancer genome atla ,06 Biological Sciences ,COMPREHENSIVE MOLECULAR CHARACTERIZATION ,Wnt Proteins ,therapeutic ,Good Health and Well Being ,030104 developmental biology ,Genes ,PanCanAtla ,biology.protein ,Cancer research ,Neoplasm ,Phosphatidylinositol 3-Kinase ,Tumor Suppressor Protein p53 ,Digestive Diseases ,Genetics and Molecular Biology(all) ,Developmental Biology - Abstract
Genetic alterations in signaling pathways that control cell-cycle progression, apoptosis, and cell growth are common hallmarks of cancer, but the extent, mechanisms, and co-occurrence of alterations in these pathways differ between individual tumors and tumor types. Using mutations, copy-number changes, mRNA expression, gene fusions and DNA methylation in 9,125 tumors profiled by The Cancer Genome Atlas (TCGA), we analyzed the mechanisms and patterns of somatic alterations in ten canonical pathways: cell cycle, Hippo, Myc, Notch, Nrf2, PI-3-Kinase/Akt, RTK-RAS, TGFβ signaling, p53 and β-catenin/Wnt. We charted the detailed landscape of pathway alterations in 33 cancer types, stratified into 64 subtypes, and identified patterns of co-occurrence and mutual exclusivity. Eighty-nine percent of tumors had at least one driver alteration in these pathways, and 57% percent of tumors had at least one alteration potentially targetable by currently available drugs. Thirty percent of tumors had multiple targetable alterations, indicating opportunities for combination therapy. An integrated analysis of genetic alterations in 10 signaling pathways in >9,000 tumors profiled by TCGA highlights significant representation of individual and co-occurring actionable alterations in these pathways, suggesting opportunities for targeted and combination therapies. Michael Seiler, Peter G. Smith, Ping Zhu, Silvia Buonamici, and Lihua Yu are employees of H3 Biomedicine, Inc. Parts of this work are the subject of a patent application: WO2017040526 titled “Splice variants associated with neomorphic sf3b1 mutants.” Shouyoung Peng, Anant A. Agrawal, James Palacino, and Teng Teng are employees of H3 Biomedicine, Inc. Andrew D. Cherniack, Ashton C. Berger, and Galen F. Gao receive research support from Bayer Pharmaceuticals. Gordon B. Mills serves on the External Scientific Review Board of Astrazeneca. Anil Sood is on the Scientific Advisory Board for Kiyatec and is a shareholder in BioPath. Jonathan S. Serody receives funding from Merck, Inc. Kyle R. Covington is an employee of Castle Biosciences, Inc. Preethi H. Gunaratne is founder, CSO, and shareholder of NextmiRNA Therapeutics. Christina Yau is a part-time employee/consultant at NantOmics. Franz X. Schaub is an employee and shareholder of SEngine Precision Medicine, Inc. Carla Grandori is an employee, founder, and shareholder of SEngine Precision Medicine, Inc. Robert N. Eisenman is a member of the Scientific Advisory Boards and shareholder of Shenogen Pharma and Kronos Bio. Daniel J. Weisenberger is a consultant for Zymo Research Corporation. Joshua M. Stuart is the founder of Five3 Genomics and shareholder of NantOmics. Marc T. Goodman receives research support from Merck, Inc. Andrew J. Gentles is a consultant for Cibermed. Charles M. Perou is an equity stock holder, consultant, and Board of Directors member of BioClassifier and GeneCentric Diagnostics and is also listed as an inventor on patent applications on the Breast PAM50 and Lung Cancer Subtyping assays. Matthew Meyerson receives research support from Bayer Pharmaceuticals; is an equity holder in, consultant for, and Scientific Advisory Board chair for OrigiMed; and is an inventor of a patent for EGFR mutation diagnosis in lung cancer, licensed to LabCorp. Eduard Porta-Pardo is an inventor of a patent for domainXplorer. Han Liang is a shareholder and scientific advisor of Precision Scientific and Eagle Nebula. Da Yang is an inventor on a pending patent application describing the use of antisense oligonucleotides against specific lncRNA sequence as diagnostic and therapeutic tools. Yonghong Xiao was an employee and shareholder of TESARO, Inc. Bin Feng is an employee and shareholder of TESARO, Inc. Carter Van Waes received research funding for the study of IAP inhibitor ASTX660 through a Cooperative Agreement between NIDCD, NIH, and Astex Pharmaceuticals. Raunaq Malhotra is an employee and shareholder of Seven Bridges, Inc. Peter W. Laird serves on the Scientific Advisory Board for AnchorDx. Joel Tepper is a consultant at EMD Serono. Kenneth Wang serves on the Advisory Board for Boston Scientific, Microtech, and Olympus. Andrea Califano is a founder, shareholder, and advisory board member of DarwinHealth, Inc. and a shareholder and advisory board member of Tempus, Inc. Toni K. Choueiri serves as needed on advisory boards for Bristol-Myers Squibb, Merck, and Roche. Lawrence Kwong receives research support from Array BioPharma. Sharon E. Plon is a member of the Scientific Advisory Board for Baylor Genetics Laboratory. Beth Y. Karlan serves on the Advisory Board of Invitae.
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- 2018
16. Italian practical clinical guidelines on cholangiocarcinoma: Part II, treatment
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Alvaro, D., Hassan, C., Cardinale, V., Carpino, G., Fabris, L., Gringeri, E., Granata, V., Mutignani, M., Morement, H., Giuliante, F., Guglielmi, A., Ridola, L., Tonini, G., Marzioni, M., Grazi, G., Guido, M., Di Giulio, E., Pantano, F., Venere, R., Bragazzi, M. C., Biancanello, F., Faccioli, J., Giannetti, A., Cintolo, M., Di Giunta, M., Gambato, M., Lasagni, A., Izzo, F., Avallone, A., Banales, J., Rossi, M., Catalano, C., Laghi, A., D'Amati, G., and Mancino, M. G.
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surgery ,distal cholangiocarcinoma ,intrahepatic cholangiocarcinoma ,liver transplantation ,locoregional treatments ,perihilar cholangiocarcinoma ,target therapies - Published
- 2020
17. Cholangiocarcinoma 2020: the next horizon in mechanisms and management
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Banales, J.M., Marin, J.J.G., LaMarca, A., Rodrigues, P.M., Khan, S.A., Roberts, L.R., Cardinale, V., Carpino, G., Andersen, J.B., Braconi, C., Calvisi, D.F., Perugorria, M.J., Fabris, L., Boulter, L, Macias, R.I.R., Gaudio, E., Alvaro, D., Gradilone, S.A., Strazzabosco, M, Marzioni, M., Coulouarn, C., Fouassier, L., Raggi, C., Invernizzi, P, Mertens, J.C., Moncsek, A., Rizvi, S., Heimbach, J., Groot Koerkamp, B. (Bas), Bruix, J. (J.), Forner, A., Bridgewater, J., Valle, J.W., Gores, G.J. (Gregory), Banales, J.M., Marin, J.J.G., LaMarca, A., Rodrigues, P.M., Khan, S.A., Roberts, L.R., Cardinale, V., Carpino, G., Andersen, J.B., Braconi, C., Calvisi, D.F., Perugorria, M.J., Fabris, L., Boulter, L, Macias, R.I.R., Gaudio, E., Alvaro, D., Gradilone, S.A., Strazzabosco, M, Marzioni, M., Coulouarn, C., Fouassier, L., Raggi, C., Invernizzi, P, Mertens, J.C., Moncsek, A., Rizvi, S., Heimbach, J., Groot Koerkamp, B. (Bas), Bruix, J. (J.), Forner, A., Bridgewater, J., Valle, J.W., and Gores, G.J. (Gregory)
- Abstract
Cholangiocarcinoma (CCA) includes a cluster of highly heterogeneous biliary malignant tumours that can arise at any point of the biliary tree. Their incidence is increasing globally, currently accounting for ~15% of all primary liver cancers and ~3% of gastrointestinal malignancies. The silent presentation of these tumours combined with their highly aggressive nature and refractoriness to chemotherapy contribute to their alarming mortality, representing ~2% of all cancer-related deaths worldwide yearly. The current diagnosis of CCA by non-invasive approaches is not accurate enough, and histological confirmation is necessary. Furthermore, the high heterogeneity of CCAs at the genomic, epigenetic and molecular levels severely compromises the efficacy of the available therapies. In the past decade, increasing efforts have been made to understand the complexity of these tumours and to develop new diagnostic tools and therapies that might help to improve patient outcomes. In this expert Consensus Statement, which is endorsed by the European Network for the Study of Cholangiocarcinoma, we aim to summarize and critically discuss the latest advances in CCA, mostly focusing on classification, cells of origin, genetic and epigenetic abnormalities, molecular alterations, biomarker discovery and treatments. Furthermore, the horizon of CCA for the next decade from 2020 onwards is highlighted.
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- 2020
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18. Primary Biliary Cholangitis management: controversies, perspectives, and daily practice implications from an expert panel
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Alvaro, D, Carpino, G, Craxi, A, Floreani, A, Moschetta, A, Invernizzi, P, Alvaro, Domenico, Carpino, Guido, Craxi, Antonio, Floreani, Annarosa, Moschetta, Antonio, Invernizzi, Pietro, Alvaro, D, Carpino, G, Craxi, A, Floreani, A, Moschetta, A, Invernizzi, P, Alvaro, Domenico, Carpino, Guido, Craxi, Antonio, Floreani, Annarosa, Moschetta, Antonio, and Invernizzi, Pietro
- Abstract
Primary biliary cholangitis (PBC) is a rare progressive immune-mediated liver disease that, if not adequately treated, may culminate in end-stage disease and need for transplantation. According to current guidelines, PBC is diagnosed in the presence of anti-mitochondrial antibodies (AMA)- or specific anti-nuclear antibodies, and of a cholestatic biochemical profile, while biopsy is recommended only in selected cases. All patients receive ursodeoxycholic acid (UDCA) in first line; the only registered second line-therapy is obeticholic acid for UDCA-inadequate responders. Despite the recent advances in understanding PBC pathogenesis and developing new treatments, many grey areas remain. Six Italian experts selected the following topics as the most urgent to address in PBC management: diagnosis and natural history of PBC: as a portion of the subjects with isolated AMA, normal alkaline phosphatase levels and no symptoms of liver disease could have PBC by histology, defining how to manage and follow this population is crucial; role of liver biopsy: recent evidence suggests that biopsy may provide relevant information for risk stratification and prediction of UDCA response, possibly facilitating personalized approaches; risk stratification: the tools for risk stratification are well established, but some issues (e.g. bile acid dosage in routine practice) remain controversial; therapy: those in more advanced stages of development are nuclear receptor modulators and fibrates, but more data are needed to plan personalized strategies. In this manuscript, for each topic, current evidence, controversies, and future perspectives are summarized with the possible implications for clinical practice.
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- 2020
19. Cholangiocarcinoma 2020: the next horizon in mechanisms and management
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Banales, JM, Marin, JJG, LaMarca, A, Rodrigues, PM, Khan, SA, Roberts, LR, Cardinale, V, Carpino, G, Andersen, JB, Braconi, C, Calvisi, DF, Perugorria, MJ, Fabris, L, Boulter, L, Macias, RIR, Gaudio, E, Alvaro, D, Gradilone, SA, Strazzabosco, M, Marzioni, M, Coulouarn, C, Fouassier, L, Raggi, C, Invernizzi, P, Mertens, JC, Moncsek, A, Rizvi, S, Heimbach, J, Groot Koerkamp, B, Bruix, J, Forner, A, Bridgewater, J, Valle, JW, Gores, GJ, Banales, JM, Marin, JJG, LaMarca, A, Rodrigues, PM, Khan, SA, Roberts, LR, Cardinale, V, Carpino, G, Andersen, JB, Braconi, C, Calvisi, DF, Perugorria, MJ, Fabris, L, Boulter, L, Macias, RIR, Gaudio, E, Alvaro, D, Gradilone, SA, Strazzabosco, M, Marzioni, M, Coulouarn, C, Fouassier, L, Raggi, C, Invernizzi, P, Mertens, JC, Moncsek, A, Rizvi, S, Heimbach, J, Groot Koerkamp, B, Bruix, J, Forner, A, Bridgewater, J, Valle, JW, and Gores, GJ
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- 2020
20. Alpha-SMA expression in hepatic stellate cells and quantitative analysis of hepatic fibrosis in cirrhosis and in recurrent chronic hepatitis after liver transplantation
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Carpino, G., Morini, S., Ginanni Corradini, S., Franchitto, A., Merli, M., Siciliano, M., Gentili, F., Onetti Muda, A., Berloco, P., Rossi, M., Attili, A.F, and Gaudio, E.
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- 2005
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21. HUMAN DUODENAL SUBMUCOSAL GLANDS CONTAIN STEM CELLS WITH POTENTIAL FOR LIVER AND PANCREATIC FATES
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Cardinale, V, Carpino, G, Safarikia, S, Overi, D, Costantini, D, Ww, Lu, Riccioni, O, Nevi, L, Zhang, W, Melandro, F, Zizzari, I, Moretti, M, Nuti, M, Maroder, M, Berloco, Pb, Forbes, S, Reid, L, Gaudio, E, and Alvaro, D
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- 2019
22. Erratum: The Immune Landscape of Cancer (Immunity (2018) 48(4) (812–830.e14), (S1074761318301213), (10.1016/j.immuni.2018.03.023))
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Thorsson, V., Gibbs, D. L., Brown, S. D., Wolf, D., Bortone, D. S., Ou Yang, T. -H., Porta-Pardo, E., Gao, G. F., Plaisier, C. L., Eddy, J. A., Ziv, E., Culhane, A. C., Paull, E. O., Sivakumar, I. K. A., Gentles, A. J., Malhotra, R., Farshidfar, F., Colaprico, A., Parker, J. S., Mose, L. E., N. S., Vo, Liu, J., Liu, Y., Rader, J., Dhankani, V., Reynolds, S. M., Bowlby, R., Califano, A., Cherniack, A. D., Anastassiou, D., Bedognetti, D., Mokrab, Y., Newman, A. M., Rao, A., Chen, K., Krasnitz, A., Hu, H., Malta, T. M., Noushmehr, H., Pedamallu, C. S., Bullman, S., Ojesina, A. I., Lamb, A., Zhou, W., Shen, H., Choueiri, T. K., Weinstein, J. N., Guinney, J., Saltz, J., Holt, R. A., Rabkin, C. S., Caesar-Johnson, S. J., Demchok, J. A., Felau, I., Kasapi, M., Ferguson, M. L., Hutter, C. M., Sofia, H. J., Tarnuzzer, R., Wang, Z., Yang, L., Zenklusen, J. C., Zhang, J. J., Chudamani, S., Lolla, L., Naresh, R., Pihl, T., Sun, Q., Wan, Y., Wu, Y., Cho, J., Defreitas, T., Frazer, S., Gehlenborg, N., Getz, G., Heiman, D. I., Kim, J., Lawrence, M. S., Lin, P., Meier, S., Noble, M. S., Saksena, G., Voet, D., Zhang, H., Bernard, B., Chambwe, N., Knijnenburg, T., Kramer, R., Leinonen, K., Miller, M., Reynolds, S., Shmulevich, I., Zhang, W., Akbani, R., Broom, B. M., Hegde, A. M., Ju, Z., Kanchi, R. S., Korkut, A., Li, J., Liang, H., Ling, S., Liu, W., Lu, Y., Mills, G. B., K. -S., Ng, Ryan, M., Wang, J., Zhang, J., Abeshouse, A., Armenia, J., Chakravarty, D., Chatila, W. K., de Bruijn, I., Gao, J., Gross, B. E., Heins, Z. J., Kundra, R., La, K., Ladanyi, M., Luna, A., Nissan, M. G., Ochoa, A., Phillips, S. M., Reznik, E., Sanchez-Vega, F., Sander, C., Schultz, N., Sheridan, R., Sumer, S. O., Sun, Y., Taylor, B. S., Anur, P., Peto, M., Spellman, P., Benz, C., Stuart, J. M., Wong, C. K., Yau, C., Hayes, D. N., Wilkerson, M. D., Ally, A., Balasundaram, M., Brooks, D., Carlsen, R., Chuah, E., Dhalla, N., Holt, R., Jones, S. J. M., Kasaian, K., Lee, D., Ma, Y., Marra, M. A., Mayo, M., Moore, R. A., Mungall, A. J., Mungall, K., Robertson, A. G., Sadeghi, S., Schein, J. E., Sipahimalani, P., Tam, A., Thiessen, N., Tse, K., Wong, T., Berger, A. C., Beroukhim, R., Cibulskis, C., Gabriel, S. B., Ha, G., Meyerson, M., Schumacher, S. E., Shih, J., Kucherlapati, M. H., Kucherlapati, R. S., Baylin, S., Cope, L., Danilova, L., Bootwalla, M. S., Lai, P. H., Maglinte, D. T., Van Den Berg, D. J., Weisenberger, D. J., Auman, J. T., Balu, S., Bodenheimer, T., Fan, C., Hoadley, K. A., Hoyle, A. P., Jefferys, S. R., Jones, C. D., Meng, S., Mieczkowski, P. A., Perou, A. H., Perou, C. M., Roach, J., Shi, Y., Simons, J. V., Skelly, T., Soloway, M. G., Tan, D., Veluvolu, U., Fan, H., Hinoue, T., Laird, P. W., Bellair, M., Chang, K., Covington, K., Creighton, C. J., Dinh, H., Doddapaneni, H., Donehower, L. A., Drummond, J., Gibbs, R. A., Glenn, R., Hale, W., Han, Y., Hu, J., Korchina, V., Lee, S., Lewis, L., Li, W., Liu, X., Morgan, M., Morton, D., Muzny, D., Santibanez, J., Sheth, M., Shinbrot, E., Wang, L., Wang, M., Wheeler, D. A., Xi, L., Zhao, F., Hess, J., Appelbaum, E. L., Bailey, M., Cordes, M. G., Ding, L., Fronick, C. C., Fulton, L. A., Fulton, R. S., Kandoth, C., Mardis, E. R., Mclellan, M. D., Miller, C. A., Schmidt, H. K., Wilson, R. K., Crain, D., Curley, E., Gardner, J., Lau, K., Mallery, D., Morris, S., Paulauskis, J., Penny, R., Shelton, C., Shelton, T., Sherman, M., Thompson, E., Yena, P., Bowen, J., Gastier-Foster, J. M., Gerken, M., Leraas, K. M., Lichtenberg, T. M., Ramirez, N. C., Wise, L., Zmuda, E., Corcoran, N., Costello, T., Hovens, C., Carvalho, A. L., de Carvalho, A. C., Fregnani, J. H., Longatto-Filho, A., Reis, R. M., Scapulatempo-Neto, C., Silveira, H. C. S., Vidal, D. O., Burnette, A., Eschbacher, J., Hermes, B., Noss, A., Singh, R., Anderson, M. L., Castro, P. D., Ittmann, M., Huntsman, D., Kohl, B., Le, X., Thorp, R., Andry, C., Duffy, E. R., Lyadov, V., Paklina, O., Setdikova, G., Shabunin, A., Tavobilov, M., Mcpherson, C., Warnick, R., Berkowitz, R., Cramer, D., Feltmate, C., Horowitz, N., Kibel, A., Muto, M., Raut, C. P., Malykh, A., Barnholtz-Sloan, J. S., Barrett, W., Devine, K., Fulop, J., Ostrom, Q. T., Shimmel, K., Wolinsky, Y., Sloan, A. E., De Rose, A., Giuliante, F., Goodman, M., Karlan, B. Y., Hagedorn, C. H., Eckman, J., Harr, J., Myers, J., Tucker, K., Zach, L. A., Deyarmin, B., Kvecher, L., Larson, C., Mural, R. J., Somiari, S., Vicha, A., Zelinka, T., Bennett, J., Iacocca, M., Rabeno, B., Swanson, P., Latour, M., Lacombe, L., Tetu, B., Bergeron, A., Mcgraw, M., Staugaitis, S. M., Chabot, J., Hibshoosh, H., Sepulveda, A., Su, T., Wang, T., Potapova, O., Voronina, O., Desjardins, L., Mariani, O., Roman-Roman, S., Sastre, X., Stern, M. -H., Cheng, F., Signoretti, S., Berchuck, A., Bigner, D., Lipp, E., Marks, J., Mccall, S., Mclendon, R., Secord, A., Sharp, A., Behera, M., Brat, D. J., Chen, A., Delman, K., Force, S., Khuri, F., Magliocca, K., Maithel, S., Olson, J. J., Owonikoko, T., Pickens, A., Ramalingam, S., Shin, D. M., Sica, G., Van Meir, E. G., Eijckenboom, W., Gillis, A., Korpershoek, E., Looijenga, L., Oosterhuis, W., Stoop, H., van Kessel, K. E., Zwarthoff, E. C., Calatozzolo, C., Cuppini, L., Cuzzubbo, S., Dimeco, F., Finocchiaro, G., Mattei, L., Perin, A., Pollo, B., Chen, C., Houck, J., Lohavanichbutr, P., Hartmann, A., Stoehr, C., Stoehr, R., Taubert, H., Wach, S., Wullich, B., Kycler, W., Murawa, D., Wiznerowicz, M., Chung, K., Edenfield, W. J., Martin, J., Baudin, E., Bubley, G., Bueno, R., De Rienzo, A., Richards, W. G., Kalkanis, S., Mikkelsen, T., Scarpace, L., Girard, N., Aymerich, M., Campo, E., Gine, E., Guillermo, A. L., Van Bang, N., Hanh, P. T., Phu, B. D., Tang, Y., Colman, H., Evason, K., Dottino, P. R., Martignetti, J. A., Gabra, H., Juhl, H., Akeredolu, T., Stepa, S., Hoon, D., Ahn, K., Kang, K. J., Beuschlein, F., Breggia, A., Birrer, M., Bell, D., Borad, M., Bryce, A. H., Castle, E., Chandan, V., Cheville, J., Copland, J. A., Farnell, M., Flotte, T., Giama, N., Ho, T., Kendrick, M., Kocher, J. -P., Kopp, K., Moser, C., Nagorney, D., O'Brien, D., O'Neill, B. P., Patel, T., Petersen, G., Que, F., Rivera, M., Roberts, L., Smallridge, R., Smyrk, T., Stanton, M., Thompson, R. H., Torbenson, M., Yang, J. D., Zhang, L., Brimo, F., Ajani, J. A., Gonzalez, A. M. A., Behrens, C., Bondaruk, J., Broaddus, R., Czerniak, B., Esmaeli, B., Fujimoto, J., Gershenwald, J., Guo, C., Lazar, A. J., Logothetis, C., Meric-Bernstam, F., Moran, C., Ramondetta, L., Rice, D., Sood, A., Tamboli, P., Thompson, T., Troncoso, P., Tsao, A., Wistuba, I., Carter, C., Haydu, L., Hersey, P., Jakrot, V., Kakavand, H., Kefford, R., Lee, K., Long, G., Mann, G., Quinn, M., Saw, R., Scolyer, R., Shannon, K., Spillane, A., Stretch, O., Synott, M., Thompson, J., Wilmott, J., Al-Ahmadie, H., Chan, T. A., Ghossein, R., Gopalan, A., Levine, D. A., Reuter, V., Singer, S., Singh, B., Tien, N. V., Broudy, T., Mirsaidi, C., Nair, P., Drwiega, P., Miller, J., Smith, J., Zaren, H., Park, J. -W., Hung, N. P., Kebebew, E., Linehan, W. M., Metwalli, A. R., Pacak, K., Pinto, P. A., Schiffman, M., Schmidt, L. S., Vocke, C. D., Wentzensen, N., Worrell, R., Yang, H., Moncrieff, M., Goparaju, C., Melamed, J., Pass, H., Botnariuc, N., Caraman, I., Cernat, M., Chemencedji, I., Clipca, A., Doruc, S., Gorincioi, G., Mura, S., Pirtac, M., Stancul, I., Tcaciuc, D., Albert, M., Alexopoulou, I., Arnaout, A., Bartlett, J., Engel, J., Gilbert, S., Parfitt, J., Sekhon, H., Thomas, G., Rassl, D. M., Rintoul, R. C., Bifulco, C., Tamakawa, R., Urba, W., Hayward, N., Timmers, H., Antenucci, A., Facciolo, F., Grazi, G., Marino, M., Merola, R., de Krijger, R., Gimenez-Roqueplo, A. -P., Piche, A., Chevalier, S., Mckercher, G., Birsoy, K., Barnett, G., Brewer, C., Farver, C., Naska, T., Pennell, N. A., Raymond, D., Schilero, C., Smolenski, K., Williams, F., Morrison, C., Borgia, J. A., Liptay, M. J., Pool, M., Seder, C. W., Junker, K., Omberg, L., Dinkin, M., Manikhas, G., Alvaro, D., Bragazzi, M. C., Cardinale, V., Carpino, G., Gaudio, E., Chesla, D., Cottingham, S., Dubina, M., Moiseenko, F., Dhanasekaran, R., Becker, K. -F., Janssen, K. -P., Slotta-Huspenina, J., Abdel-Rahman, M. H., Aziz, D., Bell, S., Cebulla, C. M., Davis, A., Duell, R., Elder, J. B., Hilty, J., Kumar, B., Lang, J., Lehman, N. L., Mandt, R., Nguyen, P., Pilarski, R., Rai, K., Schoenfield, L., Senecal, K., Wakely, P., Hansen, P., Lechan, R., Powers, J., Tischler, A., Grizzle, W. E., Sexton, K. C., Kastl, A., Henderson, J., Porten, S., Waldmann, J., Fassnacht, M., Asa, S. L., Schadendorf, D., Couce, M., Graefen, M., Huland, H., Sauter, G., Schlomm, T., Simon, R., Tennstedt, P., Olabode, O., Nelson, M., Bathe, O., Carroll, P. R., Chan, J. M., Disaia, P., Glenn, P., Kelley, R. K., Landen, C. N., Phillips, J., Prados, M., Simko, J., Smith-McCune, K., Vandenberg, S., Roggin, K., Fehrenbach, A., Kendler, A., Sifri, S., Steele, R., Jimeno, A., Carey, F., Forgie, I., Mannelli, M., Carney, M., Hernandez, B., Campos, B., Herold-Mende, C., Jungk, C., Unterberg, A., von Deimling, A., Bossler, A., Galbraith, J., Jacobus, L., Knudson, M., Knutson, T., Ma, D., Milhem, M., Sigmund, R., Godwin, A. K., Madan, R., Rosenthal, H. G., Adebamowo, C., Adebamowo, S. N., Boussioutas, A., Beer, D., Giordano, T., Mes-Masson, A. -M., Saad, F., Bocklage, T., Landrum, L., Mannel, R., Moore, K., Moxley, K., Postier, R., Walker, J., Zuna, R., Feldman, M., Valdivieso, F., Dhir, R., Luketich, J., Pinero, E. M. M., Quintero-Aguilo, M., Carlotti, C. G., Dos Santos, J. S., Kemp, R., Sankarankuty, A., Tirapelli, D., Catto, J., Agnew, K., Swisher, E., Creaney, J., Robinson, B., Shelley, C. S., Godwin, E. M., Kendall, S., Shipman, C., Bradford, C., Carey, T., Haddad, A., Moyer, J., Peterson, L., Prince, M., Rozek, L., Wolf, G., Bowman, R., Fong, K. M., Yang, I., Korst, R., Rathmell, W. K., Fantacone-Campbell, J. L., Hooke, J. A., Kovatich, A. J., Shriver, C. D., Dipersio, J., Drake, B., Govindan, R., Heath, S., Ley, T., Van Tine, B., Westervelt, P., Rubin, M. A., Lee, J. I., Aredes, N. D., Mariamidze, A., Serody, J. S., Demicco, E. G., Disis, M. L., and Vincent, B. G.
- Subjects
immune ,cancer ,methods - Published
- 2019
23. Ductular reaction, intermediate hepatocytes and fibrosis extension correlate with prediction of treatment failure to ursodeoxycholic acid in primary biliary cholangitis
- Author
-
Cristoferi, L, Carpino, G, Cardinale, V, Rigamonti, C, Overi, D, Zucchini, N, Leutner, M, Viganò, M, Nardi, A, Gerussi, A, Ronca, V, Cadamuro, M, Bonato, G, Alvaro, D, Gaudio, E, Invernizzi, P, Mells, G, Carbone, M, Mells, GF, Cristoferi, L, Carpino, G, Cardinale, V, Rigamonti, C, Overi, D, Zucchini, N, Leutner, M, Viganò, M, Nardi, A, Gerussi, A, Ronca, V, Cadamuro, M, Bonato, G, Alvaro, D, Gaudio, E, Invernizzi, P, Mells, G, Carbone, M, and Mells, GF
- Published
- 2019
24. FRI-011-Ductular reaction, intermediate hepatocites and fibrosis extension correlate with prediction of treatment failure to ursodeoxycholic acid in primary biliary cholangitis
- Author
-
Cristoferi, L, Carpino, G, Cardinale, V, Rigamonti, C, Overi, D, Zucchini, N, Leutner, M, Viganò, M, Nardi, A, Gerussi, A, Ronca, V, Cadamuro, M, Bonato, G, Alvaro, D, Gaudio, E, Jones, D, Invernizzi, P, Mells, G, Carbone, M, Cristoferi, Laura, Carpino, Guido, Cardinale, Vincenzo, Rigamonti, Cristina, Overi, Diletta, Zucchini, Nicola, Leutner, Monica, Viganò, Mauro, Nardi, Alessandra, Gerussi, Alessio, Ronca, Vincenzo, Cadamuro, Massimiliano, Bonato, Giulia, Alvaro, Domenico, Gaudio, Eugenio, Jones, David, Invernizzi, Pietro, Mells, George, Carbone, Marco, Cristoferi, L, Carpino, G, Cardinale, V, Rigamonti, C, Overi, D, Zucchini, N, Leutner, M, Viganò, M, Nardi, A, Gerussi, A, Ronca, V, Cadamuro, M, Bonato, G, Alvaro, D, Gaudio, E, Jones, D, Invernizzi, P, Mells, G, Carbone, M, Cristoferi, Laura, Carpino, Guido, Cardinale, Vincenzo, Rigamonti, Cristina, Overi, Diletta, Zucchini, Nicola, Leutner, Monica, Viganò, Mauro, Nardi, Alessandra, Gerussi, Alessio, Ronca, Vincenzo, Cadamuro, Massimiliano, Bonato, Giulia, Alvaro, Domenico, Gaudio, Eugenio, Jones, David, Invernizzi, Pietro, Mells, George, and Carbone, Marco
- Published
- 2019
25. SAT-161 - Obeticholic acid, a FXR agonist, inhibits the cancerogenic potential of primary human cholangiocarcinoma (CCA) cells cultures
- Author
-
Matteo, S.D., Nevi, L., Constantini, D., Colantonio, M., Giulitti, F., Napoletano, C., Safarikia, S., Manzi, E., Rose, A.M.D., Melandro, F., Bragazzi, M., Berloco, P.B., Giuliante, F., Carpino, G., Cardinale, V., Gaudio, E., and Alvaro, D.
- Published
- 2018
- Full Text
- View/download PDF
26. OC.01.1 BILIARY TREE STEM CELLS PLAY A KEY ROLE IN THE REGENERATION OF BILIARY EPITHELIUM AFTER INJURY
- Author
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Nevi, L., primary, Carpino, G., additional, Cardinale, V., additional, Lu, W., additional, Di Matteo, S., additional, Overi, D., additional, Safarikia, S., additional, Costantini, D., additional, Melandro, F., additional, Berloco, P.B., additional, Gaudio, E., additional, Forbes, S., additional, and Alvaro, D., additional
- Published
- 2019
- Full Text
- View/download PDF
27. PC.01.6 HUMAN DUODENAL SUBMUCOSAL GLANDS CONTAIN STEM CELLS WITH POTENTIAL FOR LIVER AND PANCREATIC FATES
- Author
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Cardinale, V., primary, Carpino, G., additional, Safarikia, S., additional, Overi, D., additional, Costantini, D., additional, Lu, W. Wei, additional, Riccioni, O., additional, Nevi, L., additional, Zhang, W., additional, Melandro, F., additional, Zizzari, I., additional, Moretti, M., additional, Nuti, M., additional, Maroder, M., additional, Berloco, P.B., additional, Forbes, S., additional, Reid, L., additional, Gaudio, E., additional, and Alvaro, D., additional
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- 2019
- Full Text
- View/download PDF
28. Human duodenal submucosal glands contain stem cells with potential for liver and pancreatic regenerative medicine
- Author
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Carpino, G., primary, Cardinale, V., additional, Safarikia, S., additional, Overi, D., additional, Costantini, D., additional, Lu, W.-Yu, additional, Riccioni, O., additional, Nevi, L., additional, Zhang, W., additional, Melandro, F., additional, Zizzarri, I., additional, Nuti, M., additional, Moretti, M., additional, Maroder, M., additional, Berloco, P.B., additional, Forbes, S.J., additional, Reid, L.M., additional, Gaudio, E., additional, and Alvaro, D., additional
- Published
- 2019
- Full Text
- View/download PDF
29. Ductular reaction, intermediate hepatocytes and fibrosis extension correlate with prediction of treatment failure to ursodeoxycholic acid in primary biliary cholangitis
- Author
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Cristoferi, L., primary, Carpino, G., additional, Cardinale, V., additional, Rigamonti, C., additional, Overi, D., additional, Zucchini, N., additional, Leutner, M., additional, Viganò, M., additional, Nardi, A., additional, Gerussi, A., additional, Ronca, V., additional, Cadamuro, M., additional, Bonato, G., additional, Alvaro, D., additional, Gaudio, E., additional, Invernizzi, P., additional, Mells, G.F., additional, and Carbone, M., additional
- Published
- 2019
- Full Text
- View/download PDF
30. The FXR agonist obeticholic acid inhibits the cancerogenic potential of human cholangiocarcinoma
- Author
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Di Matteo, S., primary, Nevi, L., additional, Costantini, D., additional, Overi, D., additional, Carpino, G., additional, Safarikia, S., additional, Giulitti, F., additional, Napoletano, C., additional, Manzi, E., additional, De Rose, A. M., additional, Melandro, F., additional, Bragazzi, M., additional, Berloco, P. B., additional, Giuliante, F., additional, Grazi, G., additional, Giorgi, A., additional, Cardinale, V., additional, Adorini, L., additional, Gaudio, E., additional, and Alvaro, D., additional
- Published
- 2019
- Full Text
- View/download PDF
31. Pretreatment prediction of response to ursodeoxycholic acid in primary biliary cholangitis: development and validation of the UDCA Response Score
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Carbone, M, Nardi, A, Flack, S, Carpino, G, Varvaropoulou, N, Gavrila, C, Spicer, A, Badrock, J, Bernuzzi, F, Cardinale, V, Ainsworth, H, Heneghan, M, Thorburn, D, Bathgate, A, Jones, R, Neuberger, J, Battezzati, P, Zuin, M, Taylor-Robinson, S, Donato, M, Kirby, J, Mitchell-Thain, R, Floreani, A, Sampaziotis, F, Muratori, L, Alvaro, D, Marzioni, M, Miele, L, Marra, F, Giannini, E, Gaudio, E, Ronca, V, Bonato, G, Cristoferi, L, Malinverno, F, Gerussi, A, Stocken, D, Cordell, H, Hirschfield, G, Alexander, G, Sandford, R, Jones, D, Invernizzi, P, Mells, G, Thomas, C, Rahman, M, Yapp, T, Lye Ch'ng, C, Harrison, M, Sturgess, R, Galaska, R, Healey, C, Whiteman, J, Czaijkowski, M, Gray, C, Gunasekera, A, Gyawli, P, Premchand, P, Mann, S, Elliott, K, Kapur, K, Watson, A, Foster, G, Trembling, P, Subhani, J, Harvey, R, Mccorry, R, Adgey, C, Hobson, L, Mulvaney-Jones, C, Evans, R, Mathialahan, T, Ramanaden, D, Gasem, J, Van Duyvenvoorde, G, Shorrock, C, Seward, K, Southern, P, Tibble, J, Penn, R, Gorard, D, Maiden, J, Damant, R, Palegwala, A, Jones, S, Dolwani, S, Prince, M, Silvestre, V, Foxton, M, Dungca, E, Mitchison, H, Wheatley, N, Gooding, I, Doyle, H, Karmo, M, Kent, M, Saksena, S, Braim, D, Patel, M, Lord, S, Ede, R, Paton, A, Austin, A, Lancaster, N, Sayer, J, Gibbins, A, Hogben, K, Hovell, C, Fisher, N, Carter, M, Koss, K, Musselwhite, J, Muscariu, F, Piotreowicz, A, Mckay, A, Grimley, C, Neal, D, Ting Tan, L, Lim, G, Brighton, J, Foale, C, Ala, A, Saeed, A, Flahive, K, Wood, G, Townshend, P, Ford, C, Brown, J, Kordula, J, Bowles, J, Wilkinson, M, Palmer, C, Ramage, J, Gordon, H, Featherstone, J, Ridpath, J, Ngatchu, T, Levi, S, Shaukat, S, Sadeghian, J, Shidrawi, R, Williams, B, Abouda, G, Duggan, C, Hynes, A, Narain, M, Rees, I, Salam, I, Crossey, M, Brown, A, Macnicol, C, Williams, S, Wilhelmsen, E, Banim, P, Raymode, P, Chilton, A, Das, D, Lee, H, Curtis, H, Gess, M, Durant, E, Drake, I, Bishop, R, Davies, M, Aldersley, M, Ncube, N, Mcnair, A, Srirajaskanthan, R, Sen, S, Casey, R, Bird, G, Mendall, M, Cowley, C, Barnardo, A, Kitchen, P, Yoong, K, Amore, K, Sirdefield, D, Orpe, J, Mathew, R, Macfaul, G, Wrigth, A, Shah, A, Evans, C, Keggans, J, Bird, B, Baxter, G, Saha, S, Pollock, K, Hughes, M, Bramley, P, Grieve, E, Young, K, Fraser, A, Mukhopadhya, A, Ocker, K, Mills, P, Hines, F, Shallcross, C, Wilkins, J, Grellier, L, Campbell, S, Martin, K, Innes, C, Shepherd, A, Rushbrook, S, Valliani, T, Przemioslo, R, Fairlamb, H, Macdonald, C, Eastick, A, Metcalf, J, Tanqueray, E, Shmueli, U, Holbrook, B, Davis, A, Browning, J, Naqvi, A, Walker, K, Lee, T, Verheyden, J, Slininger, S, Ryder, S, Chapman, R, Collier, J, O'Donnell, D, Stafford, L, Williamson, K, Kent, L, Klass, H, Ninkovic, M, March, L, Cramp, M, Simpson, D, Dickson, C, Sharer, N, Hayes, M, Goggin, P, Quinne, M, Pearson, S, Hoeroldt, B, Jones, L, Wright, A, Booth, J, Loftus, A, Lipscomb, G, Dewhurst, H, Gunter, E, Williams, E, Fouracres, A, Farrington, L, Graves, L, Hussaini, H, Stableforth, B, Marriott, S, Ayres, R, Leoni, M, Burroughs, A, Marshall, E, Tyrer, D, Lombard, M, Patanwala, I, Dali-Kemmery, L, Lambourne, V, Maltby, J, Vyas, S, Colley, J, Shinder, B, Singhal, S, Jones, J, Mills, M, Gleeson, D, Carnahan, M, Butterworth, J, Boulton, K, Taylor, N, George, K, Harding, T, Tregonning, J, Douglass, A, Brown, C, Clifford, G, Panter, S, Gocher, D, Shearman, J, Bray, G, Hamilton, M, Butcher, G, Forton, D, Mclindon, J, Curtis, J, Shewan, T, Cowan, M, Whatley, G, Nasseri, M, Grover, B, Sivaramakrishnan, N, Ducker, S, Houghton, K, Griffiths, L, Tripoli, S, Pitcher, M, Shpuza, E, White, N, Ghosh, D, Douds, A, Green, M, Brookes, M, Cumlat, L, Wong, V, Warner, K, Netherton, K, Mandal, A, Jain, S, Gupta, H, Sanghi, P, Pereira, S, Gunson, B, Lim, R, Gallagher, S, Clement, D, Brind, A, Watts, G, Mupudzi, M, Wright, M, Gitahi, J, Gordon, F, Unitt, E, Pateman, H, Batham, S, Delahooke, T, Grant, A, Conder, J, Higham, A, Cox, M, O'Donohoe, L, Currie, L, King, A, Oblak, M, Collins, C, Whalley, S, Quinn, M, Baird, Y, Amey, I, Fraser, J, Li, A, Cotterill, D, Bell, A, Singhal, A, Gee, I, Greer, S, Ang, Y, Ransford, R, Allison, J, Gotto, J, Dyer, S, Sweeting, H, Millson, C, Labbadia, G, Bragazzi, M, Andreone, P, Azzaroli, F, Galli, A, Tarocchi, M, Gasbarrini, A, Grieco, A, Marrone, G, Valenti, L, Maroni, L, Rigamonti, C, Picciotto, A, Carbone, Marco, Nardi, Alessandra, Flack, Steve, Carpino, Guido, Varvaropoulou, Nikoletta, Gavrila, Caius, Spicer, Ann, Badrock, Jonathan, Bernuzzi, Francesca, Cardinale, Vincenzo, Ainsworth, Holly F, Heneghan, Michael A, Thorburn, Douglas, Bathgate, Andrew, Jones, Rebecca, Neuberger, James M, Battezzati, Pier Maria, Zuin, Massimo, Taylor-Robinson, Simon, Donato, Maria F, Kirby, John, Mitchell-Thain, Robert, Floreani, Annarosa, Sampaziotis, Fotios, Muratori, Luigi, Alvaro, Domenico, Marzioni, Marco, Miele, Luca, Marra, Fabio, Giannini, Edoardo, Gaudio, Eugenio, Ronca, Vincenzo, Bonato, Giulia, Cristoferi, Laura, Malinverno, Federica, Gerussi, Alessio, Stocken, Deborah D, Cordell, Heather J, Hirschfield, Gideon M, Alexander, Graeme J, Sandford, Richard N, Jones, David E, Invernizzi, Pietro, Mells, George F, Thomas, Caradog, Rahman, Meshbah, Yapp, Tom, Lye Ch'ng, Chin, Harrison, Melanie, Sturgess, Richard, Galaska, Roman, Healey, Chris, Whiteman, Jessica, Czaijkowski, Marek, Gray, Catherine, Gunasekera, Anton, Gyawli, Pranab, Premchand, Purushothaman, Mann, Steven, Elliott, Keith, Kapur, Kapil, Watson, Alan, Foster, Graham, Trembling, Paul, Subhani, Javaid, Harvey, Rory, McCorry, Roger, Adgey, Carolyn, Hobson, Lucie, Mulvaney-Jones, Caroline, Evans, Richard, Mathialahan, Thiriloganathan, Ramanaden, David, Gasem, Jaber, Van Duyvenvoorde, Greta, Shorrock, Christopher, Seward, Katie, Southern, Paul, Tibble, Jeremy, Penn, Ruth, Gorard, David, Maiden, Jane, Damant, Rose, Palegwala, Altaf, Jones, Susan, Alexander, Graeme, Mells, George, Sandford, Richard, Dolwani, Sunil, Prince, Martin, Silvestre, Valeria, Foxton, Matthew, Dungca, Eleanor, Mitchison, Harriet, Wheatley, Natalie, Gooding, Ian, Doyle, Helen, Karmo, Mazn, Kent, Melanie, Saksena, Sushma, Braim, Delyth, Patel, Minesh, Lord, Susan, Ede, Roland, Paton, Alison, Austin, Andrew, Lancaster, Nicola, Sayer, Joanna, Gibbins, Andrew, Hogben, Karen, Hovell, Chris, Fisher, Neil, Carter, Martyn, Koss, Konrad, Musselwhite, Janine, Muscariu, Florin, Piotreowicz, Andrzej, McKay, Alexandra, Grimley, Charles, Neal, David, Ting Tan, Lai, Lim, Guan, Brighton, Jacqueline, Foale, Carole, Ala, Aftab, Saeed, Athar, Flahive, Kerry, Wood, Gordon, Townshend, Paula, Ford, Chris, Brown, Jonathan, Kordula, Jean, Bowles, Jane, Wilkinson, Mark, Palmer, Caroline, Ramage, John, Gordon, Harriet, Featherstone, James, Ridpath, Jo, Ngatchu, Theodore, Levi, Sass, Shaukat, Syed, Sadeghian, Joy, Shidrawi, Ray, Williams, Bronwen, Abouda, George, Jones, Sarah, Duggan, Claire, Hynes, Abigail, Narain, Mark, Rees, Ian, Salam, Imroz, Crossey, Mary, Brown, Ashley, MacNicol, Carolyn, Williams, Simon, Wilhelmsen, Elva, Banim, Paul, Raymode, Parizade, Chilton, Andrew, Das, Debasish, Lee, Hye-Jeong, Curtis, Howard, Heneghan, Michael, Gess, Markus, Durant, Emma, Drake, I. M., Bishop, Rebecca, Davies, Mervyn, Aldersley, Mark, Ncube, Noma, McNair, Alistair, Srirajaskanthan, Raj, Sen, Sambit, Casey, Rebecca, Bird, George, Mendall, Mike, Cowley, Caroline, Barnardo, Adrian, Kitchen, Paul, Yoong, Kevin, Amore, Kelly, Sirdefield, Dawn, Orpe, Jacky, Mathew, Ray, MacFaul, George, Wrigth, Aruna, Shah, Amir, Evans, Chris, Keggans, Janie, Bird, Bridget, Baxter, Gwen, Saha, Subrata, Pollock, Katharine, Hughes, Maggie, Bramley, Peter, Grieve, Emma, Young, Karin, Fraser, Andrew, Mukhopadhya, Ashis, Ocker, Kate, Mills, Peter, Hines, Francis, Shallcross, Chris, Wilkins, Joy, Grellier, Leonie, Campbell, Stewart, Martin, Kirsty, Innes, Caron, Shepherd, Alan, Rushbrook, Simon, Valliani, Talal, Przemioslo, Robert, Fairlamb, Helen, Macdonald, Chris, Eastick, Anne, Metcalf, Jane, Tanqueray, Elizabeth, Shmueli, Udi, Holbrook, Becky, Davis, Andrew, Browning, Julie, Naqvi, Asifabbas, Walker, Kirsten, Lee, Tom, Verheyden, Juliette, Slininger, Susan, Ryder, Stephen D, Chapman, Roger, Collier, Jane, O'Donnell, Denise, Stafford, Lizzie, Williamson, Kate, Kent, Linda, Klass, Howard, Ninkovic, Mary, March, Linda, Cramp, Matthew, Simpson, Diane, Dickson, Christine, Sharer, Nicholas, Hayes, Maria, Goggin, Patrick, Quinne, Mary, Pearson, Sallyanne, Hoeroldt, Barbara, Jones, Linda, Wright, Alice, Booth, Jonathan, Loftus, Alison, Lipscomb, George, Dewhurst, Hannah, Gunter, Emma, Williams, Earl, Fouracres, Anna, Farrington, Liz, Graves, Lyn, Hussaini, Hyder, Stableforth, Bill, Marriott, Suzie, Ayres, Reuben, Leoni, Marina, Burroughs, Andrew, Marshall, Eileen, Tyrer, David, Martin, Kate, Lombard, Martin, Patanwala, Imran, Dali-Kemmery, Lola, Lambourne, Victoria, Maltby, Julia, Vyas, Samir, Colley, Julie, Shinder, Bal, Singhal, Saket, Jones, Jayne, Mills, Marisa, Gleeson, Dermot, Carnahan, Mandy, Butterworth, Jeff, Boulton, Kerenza, Taylor, Natalie, George, Keith, Harding, Tim, Tregonning, Julie, Douglass, Andrew, Brown, Carly, Clifford, Gayle, Panter, Simon, Gocher, Denise, Shearman, Jeremy, Bray, Gary, Hamilton, Maria, Butcher, Graham, Forton, Daniel, Mclindon, John, Curtis, Janette, Das, Debashis, Shewan, Tracey, Cowan, Matthew, Whatley, Gregory, Nasseri, Mariam, Grover, Bob, Sivaramakrishnan, Nurani, Ducker, Samantha, Houghton, Kathryn, Jones, David, Griffiths, Laura, Tripoli, Sherill, Pitcher, Maxton, Shpuza, Ervin, White, Nikki, Ghosh, Deb, Douds, Andrew, Green, Marie, Brookes, Matthew, Cumlat, Lourdes, Wong, Voi Shim, Warner, Karen, Netherton, Kimberley, Mandal, Adtya, Jain, Snjiv, Gupta, Hemant, Sanghi, Pradeep, Pereira, Steve, Neuberger, James, Gunson, Bridget, Hirschfield, Gideon, Lim, Reina Teegan, Gallagher, Susan, Clement, Darren, Brind, Alison, Watts, Gill, Mupudzi, Mcdonald, Wright, Mark, Gitahi, Jane, Gordon, Fiona, Gocher, Denis, Unitt, Esther, Pateman, Hilary, Batham, Sally, Delahooke, Toby, Grant, Allister, Conder, Jill, Higham, Andrew, Cox, Mark, O'Donohoe, Lynn, Currie, Lynn, King, Alistair, Oblak, Metod, Collins, Carole, Whalley, Simon, Quinn, Marie, Baird, Yolanda, Amey, Isobel, Fraser, Jocelyn, Li, Andy, Cotterill, Donna, Bell, Andrew, Singhal, Amit, Gee, Ian, Greer, Sandra, Ang, Yeng, Ransford, Rupert, Allison, Joanna, Gotto, James, Dyer, Simon, Sweeting, Helen, Millson, Charles, Labbadia, Giancarlo, Bragazzi, Maria Consiglia, Andreone, Pietro, Azzaroli, Francesco, Galli, Andrea, Tarocchi, Mirko, Gasbarrini, Antonio, GRIECO, ANTONIO, Marrone, Giuseppe, Donato, Maria Francesca, Valenti, Luca, Maroni, Luca, Rigamonti, Cristina, Picciotto, Antonino, Carbone, M, Nardi, A, Flack, S, Carpino, G, Varvaropoulou, N, Gavrila, C, Spicer, A, Badrock, J, Bernuzzi, F, Cardinale, V, Ainsworth, H, Heneghan, M, Thorburn, D, Bathgate, A, Jones, R, Neuberger, J, Battezzati, P, Zuin, M, Taylor-Robinson, S, Donato, M, Kirby, J, Mitchell-Thain, R, Floreani, A, Sampaziotis, F, Muratori, L, Alvaro, D, Marzioni, M, Miele, L, Marra, F, Giannini, E, Gaudio, E, Ronca, V, Bonato, G, Cristoferi, L, Malinverno, F, Gerussi, A, Stocken, D, Cordell, H, Hirschfield, G, Alexander, G, Sandford, R, Jones, D, Invernizzi, P, Mells, G, Thomas, C, Rahman, M, Yapp, T, Lye Ch'ng, C, Harrison, M, Sturgess, R, Galaska, R, Healey, C, Whiteman, J, Czaijkowski, M, Gray, C, Gunasekera, A, Gyawli, P, Premchand, P, Mann, S, Elliott, K, Kapur, K, Watson, A, Foster, G, Trembling, P, Subhani, J, Harvey, R, Mccorry, R, Adgey, C, Hobson, L, Mulvaney-Jones, C, Evans, R, Mathialahan, T, Ramanaden, D, Gasem, J, Van Duyvenvoorde, G, Shorrock, C, Seward, K, Southern, P, Tibble, J, Penn, R, Gorard, D, Maiden, J, Damant, R, Palegwala, A, Jones, S, Dolwani, S, Prince, M, Silvestre, V, Foxton, M, Dungca, E, Mitchison, H, Wheatley, N, Gooding, I, Doyle, H, Karmo, M, Kent, M, Saksena, S, Braim, D, Patel, M, Lord, S, Ede, R, Paton, A, Austin, A, Lancaster, N, Sayer, J, Gibbins, A, Hogben, K, Hovell, C, Fisher, N, Carter, M, Koss, K, Musselwhite, J, Muscariu, F, Piotreowicz, A, Mckay, A, Grimley, C, Neal, D, Ting Tan, L, Lim, G, Brighton, J, Foale, C, Ala, A, Saeed, A, Flahive, K, Wood, G, Townshend, P, Ford, C, Brown, J, Kordula, J, Bowles, J, Wilkinson, M, Palmer, C, Ramage, J, Gordon, H, Featherstone, J, Ridpath, J, Ngatchu, T, Levi, S, Shaukat, S, Sadeghian, J, Shidrawi, R, Williams, B, Abouda, G, Duggan, C, Hynes, A, Narain, M, Rees, I, Salam, I, Crossey, M, Brown, A, Macnicol, C, Williams, S, Wilhelmsen, E, Banim, P, Raymode, P, Chilton, A, Das, D, Lee, H, Curtis, H, Gess, M, Durant, E, Drake, I, Bishop, R, Davies, M, Aldersley, M, Ncube, N, Mcnair, A, Srirajaskanthan, R, Sen, S, Casey, R, Bird, G, Mendall, M, Cowley, C, Barnardo, A, Kitchen, P, Yoong, K, Amore, K, Sirdefield, D, Orpe, J, Mathew, R, Macfaul, G, Wrigth, A, Shah, A, Evans, C, Keggans, J, Bird, B, Baxter, G, Saha, S, Pollock, K, Hughes, M, Bramley, P, Grieve, E, Young, K, Fraser, A, Mukhopadhya, A, Ocker, K, Mills, P, Hines, F, Shallcross, C, Wilkins, J, Grellier, L, Campbell, S, Martin, K, Innes, C, Shepherd, A, Rushbrook, S, Valliani, T, Przemioslo, R, Fairlamb, H, Macdonald, C, Eastick, A, Metcalf, J, Tanqueray, E, Shmueli, U, Holbrook, B, Davis, A, Browning, J, Naqvi, A, Walker, K, Lee, T, Verheyden, J, Slininger, S, Ryder, S, Chapman, R, Collier, J, O'Donnell, D, Stafford, L, Williamson, K, Kent, L, Klass, H, Ninkovic, M, March, L, Cramp, M, Simpson, D, Dickson, C, Sharer, N, Hayes, M, Goggin, P, Quinne, M, Pearson, S, Hoeroldt, B, Jones, L, Wright, A, Booth, J, Loftus, A, Lipscomb, G, Dewhurst, H, Gunter, E, Williams, E, Fouracres, A, Farrington, L, Graves, L, Hussaini, H, Stableforth, B, Marriott, S, Ayres, R, Leoni, M, Burroughs, A, Marshall, E, Tyrer, D, Lombard, M, Patanwala, I, Dali-Kemmery, L, Lambourne, V, Maltby, J, Vyas, S, Colley, J, Shinder, B, Singhal, S, Jones, J, Mills, M, Gleeson, D, Carnahan, M, Butterworth, J, Boulton, K, Taylor, N, George, K, Harding, T, Tregonning, J, Douglass, A, Brown, C, Clifford, G, Panter, S, Gocher, D, Shearman, J, Bray, G, Hamilton, M, Butcher, G, Forton, D, Mclindon, J, Curtis, J, Shewan, T, Cowan, M, Whatley, G, Nasseri, M, Grover, B, Sivaramakrishnan, N, Ducker, S, Houghton, K, Griffiths, L, Tripoli, S, Pitcher, M, Shpuza, E, White, N, Ghosh, D, Douds, A, Green, M, Brookes, M, Cumlat, L, Wong, V, Warner, K, Netherton, K, Mandal, A, Jain, S, Gupta, H, Sanghi, P, Pereira, S, Gunson, B, Lim, R, Gallagher, S, Clement, D, Brind, A, Watts, G, Mupudzi, M, Wright, M, Gitahi, J, Gordon, F, Unitt, E, Pateman, H, Batham, S, Delahooke, T, Grant, A, Conder, J, Higham, A, Cox, M, O'Donohoe, L, Currie, L, King, A, Oblak, M, Collins, C, Whalley, S, Quinn, M, Baird, Y, Amey, I, Fraser, J, Li, A, Cotterill, D, Bell, A, Singhal, A, Gee, I, Greer, S, Ang, Y, Ransford, R, Allison, J, Gotto, J, Dyer, S, Sweeting, H, Millson, C, Labbadia, G, Bragazzi, M, Andreone, P, Azzaroli, F, Galli, A, Tarocchi, M, Gasbarrini, A, Grieco, A, Marrone, G, Valenti, L, Maroni, L, Rigamonti, C, Picciotto, A, Carbone, Marco, Nardi, Alessandra, Flack, Steve, Carpino, Guido, Varvaropoulou, Nikoletta, Gavrila, Caius, Spicer, Ann, Badrock, Jonathan, Bernuzzi, Francesca, Cardinale, Vincenzo, Ainsworth, Holly F, Heneghan, Michael A, Thorburn, Douglas, Bathgate, Andrew, Jones, Rebecca, Neuberger, James M, Battezzati, Pier Maria, Zuin, Massimo, Taylor-Robinson, Simon, Donato, Maria F, Kirby, John, Mitchell-Thain, Robert, Floreani, Annarosa, Sampaziotis, Fotios, Muratori, Luigi, Alvaro, Domenico, Marzioni, Marco, Miele, Luca, Marra, Fabio, Giannini, Edoardo, Gaudio, Eugenio, Ronca, Vincenzo, Bonato, Giulia, Cristoferi, Laura, Malinverno, Federica, Gerussi, Alessio, Stocken, Deborah D, Cordell, Heather J, Hirschfield, Gideon M, Alexander, Graeme J, Sandford, Richard N, Jones, David E, Invernizzi, Pietro, Mells, George F, Thomas, Caradog, Rahman, Meshbah, Yapp, Tom, Lye Ch'ng, Chin, Harrison, Melanie, Sturgess, Richard, Galaska, Roman, Healey, Chris, Whiteman, Jessica, Czaijkowski, Marek, Gray, Catherine, Gunasekera, Anton, Gyawli, Pranab, Premchand, Purushothaman, Mann, Steven, Elliott, Keith, Kapur, Kapil, Watson, Alan, Foster, Graham, Trembling, Paul, Subhani, Javaid, Harvey, Rory, McCorry, Roger, Adgey, Carolyn, Hobson, Lucie, Mulvaney-Jones, Caroline, Evans, Richard, Mathialahan, Thiriloganathan, Ramanaden, David, Gasem, Jaber, Van Duyvenvoorde, Greta, Shorrock, Christopher, Seward, Katie, Southern, Paul, Tibble, Jeremy, Penn, Ruth, Gorard, David, Maiden, Jane, Damant, Rose, Palegwala, Altaf, Jones, Susan, Alexander, Graeme, Mells, George, Sandford, Richard, Dolwani, Sunil, Prince, Martin, Silvestre, Valeria, Foxton, Matthew, Dungca, Eleanor, Mitchison, Harriet, Wheatley, Natalie, Gooding, Ian, Doyle, Helen, Karmo, Mazn, Kent, Melanie, Saksena, Sushma, Braim, Delyth, Patel, Minesh, Lord, Susan, Ede, Roland, Paton, Alison, Austin, Andrew, Lancaster, Nicola, Sayer, Joanna, Gibbins, Andrew, Hogben, Karen, Hovell, Chris, Fisher, Neil, Carter, Martyn, Koss, Konrad, Musselwhite, Janine, Muscariu, Florin, Piotreowicz, Andrzej, McKay, Alexandra, Grimley, Charles, Neal, David, Ting Tan, Lai, Lim, Guan, Brighton, Jacqueline, Foale, Carole, Ala, Aftab, Saeed, Athar, Flahive, Kerry, Wood, Gordon, Townshend, Paula, Ford, Chris, Brown, Jonathan, Kordula, Jean, Bowles, Jane, Wilkinson, Mark, Palmer, Caroline, Ramage, John, Gordon, Harriet, Featherstone, James, Ridpath, Jo, Ngatchu, Theodore, Levi, Sass, Shaukat, Syed, Sadeghian, Joy, Shidrawi, Ray, Williams, Bronwen, Abouda, George, Jones, Sarah, Duggan, Claire, Hynes, Abigail, Narain, Mark, Rees, Ian, Salam, Imroz, Crossey, Mary, Brown, Ashley, MacNicol, Carolyn, Williams, Simon, Wilhelmsen, Elva, Banim, Paul, Raymode, Parizade, Chilton, Andrew, Das, Debasish, Lee, Hye-Jeong, Curtis, Howard, Heneghan, Michael, Gess, Markus, Durant, Emma, Drake, I. M., Bishop, Rebecca, Davies, Mervyn, Aldersley, Mark, Ncube, Noma, McNair, Alistair, Srirajaskanthan, Raj, Sen, Sambit, Casey, Rebecca, Bird, George, Mendall, Mike, Cowley, Caroline, Barnardo, Adrian, Kitchen, Paul, Yoong, Kevin, Amore, Kelly, Sirdefield, Dawn, Orpe, Jacky, Mathew, Ray, MacFaul, George, Wrigth, Aruna, Shah, Amir, Evans, Chris, Keggans, Janie, Bird, Bridget, Baxter, Gwen, Saha, Subrata, Pollock, Katharine, Hughes, Maggie, Bramley, Peter, Grieve, Emma, Young, Karin, Fraser, Andrew, Mukhopadhya, Ashis, Ocker, Kate, Mills, Peter, Hines, Francis, Shallcross, Chris, Wilkins, Joy, Grellier, Leonie, Campbell, Stewart, Martin, Kirsty, Innes, Caron, Shepherd, Alan, Rushbrook, Simon, Valliani, Talal, Przemioslo, Robert, Fairlamb, Helen, Macdonald, Chris, Eastick, Anne, Metcalf, Jane, Tanqueray, Elizabeth, Shmueli, Udi, Holbrook, Becky, Davis, Andrew, Browning, Julie, Naqvi, Asifabbas, Walker, Kirsten, Lee, Tom, Verheyden, Juliette, Slininger, Susan, Ryder, Stephen D, Chapman, Roger, Collier, Jane, O'Donnell, Denise, Stafford, Lizzie, Williamson, Kate, Kent, Linda, Klass, Howard, Ninkovic, Mary, March, Linda, Cramp, Matthew, Simpson, Diane, Dickson, Christine, Sharer, Nicholas, Hayes, Maria, Goggin, Patrick, Quinne, Mary, Pearson, Sallyanne, Hoeroldt, Barbara, Jones, Linda, Wright, Alice, Booth, Jonathan, Loftus, Alison, Lipscomb, George, Dewhurst, Hannah, Gunter, Emma, Williams, Earl, Fouracres, Anna, Farrington, Liz, Graves, Lyn, Hussaini, Hyder, Stableforth, Bill, Marriott, Suzie, Ayres, Reuben, Leoni, Marina, Burroughs, Andrew, Marshall, Eileen, Tyrer, David, Martin, Kate, Lombard, Martin, Patanwala, Imran, Dali-Kemmery, Lola, Lambourne, Victoria, Maltby, Julia, Vyas, Samir, Colley, Julie, Shinder, Bal, Singhal, Saket, Jones, Jayne, Mills, Marisa, Gleeson, Dermot, Carnahan, Mandy, Butterworth, Jeff, Boulton, Kerenza, Taylor, Natalie, George, Keith, Harding, Tim, Tregonning, Julie, Douglass, Andrew, Brown, Carly, Clifford, Gayle, Panter, Simon, Gocher, Denise, Shearman, Jeremy, Bray, Gary, Hamilton, Maria, Butcher, Graham, Forton, Daniel, Mclindon, John, Curtis, Janette, Das, Debashis, Shewan, Tracey, Cowan, Matthew, Whatley, Gregory, Nasseri, Mariam, Grover, Bob, Sivaramakrishnan, Nurani, Ducker, Samantha, Houghton, Kathryn, Jones, David, Griffiths, Laura, Tripoli, Sherill, Pitcher, Maxton, Shpuza, Ervin, White, Nikki, Ghosh, Deb, Douds, Andrew, Green, Marie, Brookes, Matthew, Cumlat, Lourdes, Wong, Voi Shim, Warner, Karen, Netherton, Kimberley, Mandal, Adtya, Jain, Snjiv, Gupta, Hemant, Sanghi, Pradeep, Pereira, Steve, Neuberger, James, Gunson, Bridget, Hirschfield, Gideon, Lim, Reina Teegan, Gallagher, Susan, Clement, Darren, Brind, Alison, Watts, Gill, Mupudzi, Mcdonald, Wright, Mark, Gitahi, Jane, Gordon, Fiona, Gocher, Denis, Unitt, Esther, Pateman, Hilary, Batham, Sally, Delahooke, Toby, Grant, Allister, Conder, Jill, Higham, Andrew, Cox, Mark, O'Donohoe, Lynn, Currie, Lynn, King, Alistair, Oblak, Metod, Collins, Carole, Whalley, Simon, Quinn, Marie, Baird, Yolanda, Amey, Isobel, Fraser, Jocelyn, Li, Andy, Cotterill, Donna, Bell, Andrew, Singhal, Amit, Gee, Ian, Greer, Sandra, Ang, Yeng, Ransford, Rupert, Allison, Joanna, Gotto, James, Dyer, Simon, Sweeting, Helen, Millson, Charles, Labbadia, Giancarlo, Bragazzi, Maria Consiglia, Andreone, Pietro, Azzaroli, Francesco, Galli, Andrea, Tarocchi, Mirko, Gasbarrini, Antonio, GRIECO, ANTONIO, Marrone, Giuseppe, Donato, Maria Francesca, Valenti, Luca, Maroni, Luca, Rigamonti, Cristina, and Picciotto, Antonino
- Abstract
Background: Treatment guidelines recommend a stepwise approach to primary biliary cholangitis: all patients begin treatment with ursodeoxycholic acid (UDCA) monotherapy and those with an inadequate biochemical response after 12 months are subsequently considered for second-line therapies. However, as a result, patients at the highest risk can wait the longest for effective treatment. We determined whether UDCA response can be accurately predicted using pretreatment clinical parameters. Methods: We did logistic regression analysis of pretreatment variables in a discovery cohort of patients in the UK with primary biliary cholangitis to derive the best-fitting model of UDCA response, defined as alkaline phosphatase less than 1·67 times the upper limit of normal (ULN), measured after 12 months of treatment with UDCA. We validated the model in an external cohort of patients with primary biliary cholangitis and treated with UDCA in Italy. Additionally, we assessed correlations between model predictions and key histological features, such as biliary injury and fibrosis, on liver biopsy samples. Findings: 2703 participants diagnosed with primary biliary cholangitis between Jan 1, 1998, and May 31, 2015, were included in the UK-PBC cohort for derivation of the model. The following pretreatment parameters were associated with lower probability of UDCA response: higher alkaline phosphatase concentration (p<0·0001), higher total bilirubin concentration (p=0·0003), lower aminotransferase concentration (p=0·0012), younger age (p<0·0001), longer interval from diagnosis to the start of UDCA treatment (treatment time lag, p<0·0001), and worsening of alkaline phosphatase concentration from diagnosis (p<0·0001). Based on these variables, we derived a predictive score of UDCA response. In the external validation cohort, 460 patients diagnosed with primary biliary cholangitis were treated with UDCA, with follow-up data until May 31, 2016. In this validation cohort, the area under the re
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- 2018
32. Pre-treatment risk stratification in primary biliary cholangitis: A predictive model to guide first-line combination therapy
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Carbone, M, Nardi, A, Carpino, G, Cardinale, V, Ainsworth, H, Heneghan, M, Thorburn, D, Bathgate, A, Jones, R, Neuberger, J, Battezzati, P, Zuin, M, Taylor-Robinson, S, Donato, M, Kirby, J, Mitchell-Thain, R, Floreani, A, Sampaziotis, F, Muratori, L, Alvaro, D, Marzioni, M, Miele, L, Marra, F, Giannini, E, Gaudio, E, Ronca, V, Bonato, G, Cristoferi, L, Malinverno, F, Gerussi, A, Cordell, H, Hirschfield, G, Stocken, D, Alexander, G, Sandford, R, Jones, D, Invernizzi, P, Mells, G, Neuberger, JM, Battezzati, PM, Cordell, HJ, Hirschfield, GM, Alexander, GJ, Sandford, RN, Jones, DE, Mells, GF, Carbone, M, Nardi, A, Carpino, G, Cardinale, V, Ainsworth, H, Heneghan, M, Thorburn, D, Bathgate, A, Jones, R, Neuberger, J, Battezzati, P, Zuin, M, Taylor-Robinson, S, Donato, M, Kirby, J, Mitchell-Thain, R, Floreani, A, Sampaziotis, F, Muratori, L, Alvaro, D, Marzioni, M, Miele, L, Marra, F, Giannini, E, Gaudio, E, Ronca, V, Bonato, G, Cristoferi, L, Malinverno, F, Gerussi, A, Cordell, H, Hirschfield, G, Stocken, D, Alexander, G, Sandford, R, Jones, D, Invernizzi, P, Mells, G, Neuberger, JM, Battezzati, PM, Cordell, HJ, Hirschfield, GM, Alexander, GJ, Sandford, RN, Jones, DE, and Mells, GF
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- 2018
33. Anatomia Umana. Raccolta di quesiti a risposta multipla per la verifica e l'autoverifica degli apprendimenti SSD BIO-16
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Bandiera, P., Bucchieri, F., Carpino, G., Castaldo, C., Cavaletti, G., Conconi, M. T., Consalez, G., Cremona, O., CUSELLA DE ANGELIS, M. G., DE LUCA, A., DI MEGLIO, F., YUNG FOLLO, M., Franchitto, A., Giampà, C., Manzoli, L., Mazzone, V., Morini, S., Nurzynska, D., Onori, P., Papa, M., Paternostro, F., Raspanti, M., Relucenti, M., Rezzani, R., Rizzi, A., Rodella, L. F., Rumio, C., Toesca, A., Tortorella, C., Vercelli, A., and Zecchi, S.
- Subjects
anatomia umana - Published
- 2017
34. An Adaptive Arm-Weight Support Platform for 3D Upper Limb Robot-Aided Neuro-Rehabilitation
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di Luzio, F. Scotto, primary, Simonetti, D., additional, Cordella, F., additional, Carpino, G., additional, Draicchio, F., additional, and Zollo, L., additional
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- 2018
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35. Obeticholic acid, a FXR agonist, inhibits the cancerogenic potential of primary human cholangiocarcinoma (CCA) cells cultures
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Matteo, S.D., primary, Nevi, L., additional, Constantini, D., additional, Colantonio, M., additional, Giulitti, F., additional, Napoletano, C., additional, Safarikia, S., additional, Manzi, E., additional, Rose, A.M.D., additional, Melandro, F., additional, Bragazzi, M., additional, Berloco, P.B., additional, Giuliante, F., additional, Carpino, G., additional, Cardinale, V., additional, Gaudio, E., additional, and Alvaro, D., additional
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- 2018
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36. Development of self-renewing 3D organoid culture from human fetal biliary tree stem cells (hBTSCs) as a potential system for regenerative medicine and disease modelling
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Safarikia, S., primary, Cardinale, V., additional, Carpino, G., additional, Costantini, D., additional, DI Matteo, S., additional, Nevi, L., additional, Bosco, D., additional, Gaudio, E., additional, and Alvaro, D., additional
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- 2018
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37. Peribiliary glands and biliary tree stem cells are involved in the pathogenesis of cholangiocarcinoma arising in patients affected by primary sclerosing cholangitis
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Overi, D., primary, Cardinale, V., additional, Folseraas, T., additional, Carpino, G., additional, Grzyb, K., additional, Costantini, D., additional, Berloco, P.B., additional, Alvaro, D., additional, and Gaudio, E., additional
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- 2018
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38. Different micro-environtmental factors induce proliferation, epithelial-mesenchymal transition (EMT) and senescence of primary cultures of human biliary tree stem/progenitor cells (hBTSCs), recapitulating the pathological features typical of human cholangiopathies
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Costantini, D., primary, Carpino, G., additional, Cardinale, V., additional, Overi, D., additional, Nevi, L., additional, Di Matteo, S., additional, Safarikia, S., additional, Melandro, F., additional, Berloco, P.B., additional, Gaudio, E., additional, and Alvaro, D., additional
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- 2018
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39. Pre-treatment risk stratification in primary biliary cholangitis: a predictive model to guide first-line combination therapy
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Carbone, M., primary, Nardi, A., additional, Carpino, G., additional, Heneghan, M., additional, Thorburn, D., additional, Taylor-Robinson, S., additional, Bathgate, A., additional, Zuin, M., additional, Battezzati, P.M., additional, Floreani, A., additional, Giannini, E.G., additional, Donato, M.F., additional, Marzioni, M., additional, Alvaro, D., additional, Miele, L., additional, Marra, F., additional, Ainsworth, H., additional, Muratori, L., additional, Bonato, G., additional, Ronca, V., additional, Cristoferi, L., additional, Stocken, D., additional, Cardinale, V., additional, Hirschfield, G., additional, Alexander, G.J.M., additional, Sandford, R., additional, Jones, D., additional, Invernizzi, P., additional, and Mells, G., additional
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- 2018
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40. OC.08.2 THE FXR AGONIST, OBETICHOLIC ACID, INHIBITS THE CANCEROGENIC POTENTIAL OF PRIMARY HUMAN CHOLANGIOCARCINOMA CELLS: A STUDY ON PRIMARY HUMAN CELL CULTURES
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Di Matteo, S., primary, Nevi, L., additional, Costantini, D., additional, Colantonio, M., additional, Giulitti, F., additional, Napoletano, C., additional, Safarikia, S., additional, Manzi, E., additional, Derose, A.M., additional, Meandro, F., additional, Bragazzi, M.C., additional, Grazi, G., additional, Berloco, P.B., additional, Giuliante, F., additional, Carpino, G., additional, Cardinale, V., additional, Gaudio, E., additional, and Alvaro, D., additional
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- 2018
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41. P.04.6 PRIMARY HUMAN BILIARY TREE STEM/PROGENITOR CELLS (HBTSCS) EXPOSED TO MICROENVIRONMENTAL FACTORS SHOWED PROLIFERATION, EPITHELIAL-MESENCHYMAL TRANSITION (EMT) AND SENESCENCE, RECAPITULATING THE PATHOLOGICAL FEATURES TYPICAL OF HUMAN CHOLANGIOPATHIES
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Costantini, D., primary, Cardinale, V., additional, Carpino, G., additional, Nevi, L., additional, Di Matteo, S., additional, Safarikia, S., additional, Melandro, F., additional, Berloco, P.B., additional, Gaudio, E., additional, and Alvaro, D., additional
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- 2018
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42. OC.04.1 GENERATION OF 3D ORGANOIDS OF HUMAN FETAL BILIARY TREE STEM CELLS (HBTSCS) AS INNOVATIVE TOOL FOR THE REGENERATIVE MEDICINE OF LIVER AND PANCREAS
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Safarikia, S., primary, Cardinale, V., additional, Costantini, D., additional, Di Matteo, S., additional, Nevi, L., additional, Carpino, G., additional, Bosco, D., additional, and Alvaro, D., additional
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- 2018
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43. OC.08.1 DOUBLECORTIN-LIKE KINASE 1 (DCLK1) IS A MARKER OF SPECIFIC SUBPOPULATIONS OF CANCER STEM CELLS (CSCS) IN HUMAN CHOLANGIOCARCINOMA (CCA) AND ITS INHIBITION EXERTS ANTI-CANCER EFFECTS
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Nevi, L., primary, Di Matteo, S., additional, Carpino, G., additional, Cardinale, V., additional, Ambrosino, V., additional, Costantini, D., additional, Safarikia, S., additional, Manzi, E., additional, De Rosa, A.M., additional, Melandro, F., additional, Bragazzi, M.C., additional, Grazi, G., additional, Berloco, P.B., additional, Giuliante, F., additional, Gaudio, E., additional, and Alvaro, D., additional
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- 2018
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44. Specific human cholangiocarcinoma (CCA) subpopulations of cancer stem cells (CSCs) express DoubleCortin-Like Kinase 1 (DCLK1) and DCLK1 inhibition induces anti-cancer effects
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Nevi, L., primary, Di Matteo, S., additional, Carpino, G., additional, Cardinale, V., additional, Zizzari, I., additional, Ambrosino, V., additional, Costantini, D., additional, Safarikia, S., additional, Manzi, E., additional, Derose, A.M., additional, Melandro, F., additional, Bragazzi, M.C., additional, Grazi, G., additional, Berloco, P.B., additional, Giuliante, F., additional, Gaudio, E., additional, and Alvaro, D., additional
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- 2018
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45. Establishment of expanding 3D-organoids cultures from human fetal biliary tree stem cells (hBTSCs) as a potential tool for regenerative medicine and disease modeling
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Safarikia, S., primary, Cardinale, V., additional, Carpino, G., additional, Costantini, D., additional, Di Matteo, S., additional, Nevi, L., additional, Bosco, D., additional, Gaudio, E., additional, and Alvaro, D., additional
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- 2018
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46. The cancerogenic potential of primary human Cholangioracinoma cells is inhibited by Obeticholic Acid, a Farnesoid X Receptor (FXR) agonist
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Di Matteo, S., primary, Nevi, L., additional, Costantini, D., additional, Colantonio, M., additional, Giulitti, F., additional, Napoletano, C., additional, Safarikia, S., additional, Manzi, E., additional, Derose, A.M., additional, Melandro, F., additional, Bragazzi, M.C., additional, Grazi, G., additional, Berloco, P.B., additional, Giuliante, F., additional, Carpino, G., additional, Cardinale, V., additional, Gaudio, E., additional, and Alvaro, D., additional
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- 2018
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47. The exposure of primary cultures of human biliary tree stem/progenitor cells (hBTSCs) to different micro-environmental factors induces proliferation, epithelial-mesenchymal transition (EMT) and senescence, which are typical pathological features of human cholangiopathies
- Author
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Costantini, D., primary, Cardinale, V., additional, Carpino, G., additional, Nevi, L., additional, Di Matteo, S., additional, Safarikia, S., additional, Melandro, F., additional, Berloco, P., additional, Gaudio, E., additional, and Alvaro, D., additional
- Published
- 2018
- Full Text
- View/download PDF
48. Taurocholate Feeding to Bile Duct Ligated Rats Prevents Caffeic Acid-Induced Bile Duct Damage by Changes in Cholangiocyte VEGF Expression
- Author
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Mancinelli, Romina, Onori, Paolo, Gaudio, Eugenio, Franchitto, Antonio, Carpino, G., Ueno, Y., Alvaro, Domenico, Pannarale, Luigi, Annarale, L. P., Demorrow, S., and Francis, H.
- Subjects
Male ,Taurocholic Acid ,Cholagogues and Choleretics ,medicine.medical_specialty ,Cholangiocyte proliferation ,Apoptosis ,Article ,General Biochemistry, Genetics and Molecular Biology ,Cholangiocyte ,chemistry.chemical_compound ,Caffeic Acids ,Internal medicine ,medicine ,Animals ,Bile ,Caffeic acid phenethyl ester ,Autocrine signalling ,Ligation ,vegf ,Cell Proliferation ,bile acids ,Cytotoxins ,Vascular Endothelial Growth Factors ,Bile duct ,Biliary hyperplasia ,intrahepatic biliary epithelium ,apoptosis ,biliary hyperplasia ,Phenylethyl Alcohol ,Taurocholic acid ,Immunohistochemistry ,Rats, Inbred F344 ,Rats ,Receptors, Vascular Endothelial Growth Factor ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Bile Ducts - Abstract
Cholangiocytes are the target cells in cholestatic models of ductal hyperplasia including bile duct ligation (BDL). We have shown that: (i) cholangiocytes express VEGFR-2 and VEGFR-3; (ii) VEGF-A and VEGF-C stimulate cholangiocyte proliferation via an autocrine mechanism; and (iii) chronic administration of VEGF-A prevents cholangiocyte damage induced by hepatic artery ligation. Caffeic acid phenethyl ester (CAPE) induces growth inhibition in different cells. Taurocholic acid (TC) protects cholangiocytes against injury induced by parasympathetic or sympathetic denervation. The aims of this study were to determine if: (i) CAPE induces bile duct damage; and (ii) TC prevents CAPE-induced bile duct damage by increasing cholangiocyte VEGF expression. Methods: Normal and BDL rats (immediately after surgery) were fed 1% TC or control diet in the absence/presence of daily IP injections of CAPE (10 mg/Kg BW). One week later, we evaluated: (i) cholangiocyte apoptosis, proliferation and ductal mass in liver sections; (ii) functional activity by measuring secretin-stimulated bile and bicarbonate secretion; and (iii) VEGF-A/C and VEGFR-2/R-3 expression in liver sections. In vitro, BDL cholangiocytes were exposed to CAPE (40 μM) in the absence/presence of TC (40 μM) with and without pretreatment with VEGF receptor inhibitors before evaluating cholangiocyte apoptosis and proliferation. Results: Chronic CAPE administration to BDL rats increased cholangiocyte apoptosis and decreased ductal mass. This effect was associated with reduced expression of VEGF-A, VEGF-C, VEGFR-2 and VEGFR-3. In vivo, TC feeding partly prevented CAPE-induced changes in cholangiocyte apoptosis and growth and loss of ductal secretion. The protective effect of TC was associated with enhanced VEGF-A, VEGF-C, VEGFR-2 and VEGFR-3. In vitro, TC partially prevented CAPE-induced increases in apoptosis and decreases in cholangiocyte proliferation. These changes were reversed by pretreatment with VEGF-receptor inhibitors. Conclusion: Manipulation of cholangiocyte VEGF expression by bile acids may be important in preventing the impairment of cholangiocyte proliferation by exogenous agents.
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- 2009
49. P.10.4: The Differentiation and Metabolism of Human Hepatic and Biliary Tree Stem/Progenitor Cells can be Significantly Modulated by Microgravity
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Costantini, D., primary, Cardinale, V., additional, Casadei, L., additional, Carpino, G., additional, Nevi, L., additional, Di Matteo, S., additional, Lustri, A.M., additional, Verdesca, L., additional, Melandro, F., additional, Berloco, P.B., additional, Manetti, C., additional, and Alvaro, D., additional
- Published
- 2017
- Full Text
- View/download PDF
50. P.10.2: Hyaluronic Acid Improves the Engraftment Efficiency of Human Biliary Tree Stem/Progenitor Cells (HBTSCS)
- Author
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Nevi, L., primary, Cardinale, V., additional, Carpino, G., additional, Costantini, D., additional, Di Matteo, S., additional, Safarikia, S., additional, Melandro, F., additional, Berloco, P.B., additional, Reid, L., additional, Gaudio, E., additional, and Alvaro, D., additional
- Published
- 2017
- Full Text
- View/download PDF
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