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The 8-7 crystalline-electric-field (CEF) transition of CeB6 has been identified near 530 K (46 meV, 372 cm-1) with use of inelastic neutron and polarized Raman scattering. From the anomalous temperature behavior of the transition energy observed in Raman scattering we deduce a 8 ground state split by 20 cm-1 (30 K). The novel CEF level scheme yields a consistent and unified interpretation of so far seemingly unrelated thermal, elastic, and magnetic data. © 1984 The American Physical Society.

An inelastic electronic excitation has been observed in CeB6 near 47 meV (545 K) by means of neutron and Raman spectroscopy. This excitation has been identified as the Γ8→Γ7 crystalline-electric-field (CEF) transition. From the anomalous energy shift of this excitation at low temperatures, detectable due to the high resolution of Raman spectroscopy (±5 K), we deduce a Γ8 ground-state split by about 30 K. With this new CEF level scheme the first consistent interpretation of so far seemingly unrelated thermal, elastic, and magnetic data is achieved. In spite of the large body of thermal, magnetic, and elastic data of CeB6 accumulated over the past years, various diverging proposals for the CEF level scheme have been reported. In all schemes a Γ7 ground state was assumed, but the CEF splittings ranged from 10 K6 to more than 400 K. The absence of CEF excitations in direct spectroscopic measurements up to 400 K has been puzzling, and pointed to the necessity of high-energy neutron experiments. We have performed inelastic magnetic neutron scattering experiments using high-energy incident neutrons up to 185 meV from the Intense Pulsed Neutron Source at Argonne National Laboratory. An inelastic peak at 46 meV (530 K) is clearly identified as magnetic scattering by its Q dependence. The absolute intensity corresponds to the value of a Γ8→Γ7 transition. In addition, we have carried out Raman measurements on (100) faces of CeB6. At room temperature we found an inelastic excitation at 372 cm−1 (530 K). It is absent in the reference compound LaB6. From the polarization analysis we found that the transition has Γ3+ and Γ5+ symmetry. Both facts characterize this excitation as the Γ8→Γ7 CEF transition, which is the first CEF excitation seen in a metal by means of Raman spectroscopy. The high resolution of Raman spectroscopy enabled us to detect a 10-cm−1 shift of the Γ8→Γ7 transition energy to 382 cm−1 for temperatures below 20 K. This can only be explained by assuming a Γ8 ground state, which is split by 30 K. From the spectroscopic data we can establish a completely new CEF level scheme for CeB6 with a Γ8 ground state, split by about 30 K and a Γ7 state 545 K above. This allows a straightforward interpretation of various other experimental data, such as magnetic entropy, static magnetic susceptibility, high-field magnetization, magnetic form factor,8 temperature, and magnetic-field-dependent elastic constants and the antiferroquadrupolar ordering below TQ =3.3 K. A recent theoretical investigation of magnetic ordering of a periodic Anderson Hamiltonian with orbital degeneracy has been applied to CeB6. On the basis of entropy and magnetization data it was assumed that the quartet Γ8 is the ground state. Hence it was predicted that the low-temperature phase II (2.1 K

Background: Mesenchymal stromal cell derived extracellular vesicles (MSC-EVs) are a promising therapeutic for neuroinflammation. MSC-EVs can interact with microglia, the resident immune cells of the brain, to exert their immunomodulatory effects. In response to inflammatory cues, such as cytokines, microglia undergo phenotypic changes indicative of their function e.g. morphology and secretion. However, these changes in response to MSC-EVs are not well understood. Additionally, no disease-relevant screening tools to assess MSC-EV bioactivity exist, which has further impeded clinical translation. Here, we developed a quantitative, high throughput morphological profiling approach to assess the response of microglia to neuroinflammation- relevant signals and whether this morphological response can be used to indicate the bioactivity of MSC-EVs., Results: Using an immortalized human microglia cell-line, we observed increased size (perimeter, major axis length) and complexity (form factor) upon stimulation with interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α). Upon treatment with MSC-EVs, the overall morphological score (determined using principal component analysis) shifted towards the unstimulated morphology, indicating that MSC-EVs are bioactive and modulate microglia. The morphological effects of MSC-EVs in TNF-α /IFN-γ stimulated cells were concomitant with reduced secretion of 14 chemokines/cytokines (e.g. CXCL6, CXCL9) and increased secretion of 12 chemokines/cytokines (e.g. CXCL8, CXCL10). Proteomic analysis of cell lysates revealed significant increases in 192 proteins (e.g. HIBADH, MEAK7, LAMC1) and decreases in 257 proteins (e.g. PTEN, TOM1, MFF) with MSC-EV treatment. Of note, many of these proteins are involved in regulation of cell morphology and migration. Gene Set Variation Analysis revealed upregulation of pathways associated with immune response, such as regulation of cytokine production, immune cell infiltration (e.g. T cells, NK cells) and morphological changes (e.g. Semaphorin, RHO/Rac signaling). Additionally, changes in microglia mitochondrial morphology were measured suggesting that MSC-EV modulate mitochondrial metabolism., Conclusion: This study comprehensively demonstrates the effects of MSC-EVs on human microglial morphology, cytokine secretion, cellular proteome, and mitochondrial content. Our high-throughput, rapid, low-cost morphometric approach enables screening of MSC-EV batches and manufacturing conditions to enhance EV function and mitigate EV functional heterogeneity in a disease relevant manner. This approach is highly generalizable and can be further adapted and refined based on selection of the disease-relevant signal, target cell, and therapeutic product., (© 2024. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)

γ-aminobutyric acid (GABA) is an abundant neurotransmitter that plays multiple roles in the vertebrate central nervous system (CNS). In the early developing CNS, GABAergic signaling acts to depolarize cells. It mediates several aspects of neural development, including cell proliferation, neuronal migration, neurite growth, and synapse formation, as well as the development of critical periods. Later in CNS development, GABAergic signaling acts in an inhibitory manner when it becomes the predominant inhibitory neurotransmitter in the brain. This behavior switch occurs due to changes in chloride/cation transporter expression. Abnormalities of GABAergic signaling appear to underlie several human neurological conditions, including seizure disorders. However, the impact of reduced GABAergic signaling on brain development has been challenging to study in mammals. Here we take advantage of zebrafish and light sheet imaging to assess the impact of reduced GABAergic signaling on the functional circuitry in the larval zebrafish optic tectum. Zebrafish have three gad genes: two gad1 paralogs known as gad1a and gad1b , and gad2. The gad1b and gad2 genes are expressed in the developing optic tectum. Null mutations in gad1b significantly reduce GABA levels in the brain and increase electrophysiological activity in the optic tectum. Fast light sheet imaging of genetically encoded calcium indicator (GCaMP)-expressing gab1b null larval zebrafish revealed patterns of neural activity that were different than either gad1b-normal larvae or gad1b -normal larvae acutely exposed to pentylenetetrazole (PTZ). These results demonstrate that reduced GABAergic signaling during development increases functional connectivity and concomitantly hyper-synchronization of neuronal networks.

Gulf War Illness (GWI) affects nearly 30% of veterans from the 1990-1991 Gulf War (GW) and is a multi-symptom illness with many neurological effects attributed to in-theater wartime chemical overexposures. Brain-focused studies have revealed persistent structural and functional alterations in veterans with GWI, including reduced volumes, connectivity, and signaling that correlate with poor cognitive and motor performance. GWI symptomology components have been recapitulated in rodent models as behavioral, neurochemical, and neuroinflammatory aberrations. However, preclinical structural imaging studies remain limited. This study aimed to characterize the progression of brain structural alterations over the course of 12 months in two established preclinical models of GWI. In the PB/PM model, male C57BL/6 J mice (8-9 weeks) received daily exposure to the nerve agent prophylactic pyridostigmine bromide (PB) and the pyrethroid insecticide permethrin (PM) for 10 days. In the PB/DEET/CORT/DFP model, mice received daily exposure to PB and the insect repellent DEET (days 1-14) and corticosterone (CORT; days 7-14). On day 15, mice received a single injection of the sarin surrogate diisopropylfluorophosphate (DFP). Using a Varian 7 T Bore MRI System, structural (sagittal T2-weighted) scans were performed at 6-, 9-, and 12-months post GWI exposures. Regions of interest, including total brain, ventricles, cortex, hippocampus, cerebellum, and brainstem were delineated in the open source Aedes Toolbox in MATLAB, followed by brain volumetric and cortical thickness analyses in ImageJ. Limited behavioral testing 1 month after the last MRI was also performed. The results of this study compare similarities and distinctions between these exposure paradigms and aid in the understanding of GWI pathogenesis. Major similarities among the models include relative ventricular enlargement and reductions in hippocampal volumes with age. Key differences in the PB/DEET/CORT/DFP model included reduced brainstem volumes and an early and persistent loss of total brain volume, while the PB/PM model produced reductions in cortical thickness with age. Behaviorally, at 13 months, motor function was largely preserved in both models. However, the GWI mice in the PB/DEET/CORT/DFP model exhibited an elevation in anxiety-like behavior., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Carpenter, Hughes and Filipov.)

Tetraopes longhorn beetles are known for their resistance to milkweed plant toxins and their coevolutionary dynamics with milkweed plants (Asclepias). This association is considered a textbook example of coevolution, in which each species of Tetraopes is specialized to feed on one or a few species of Asclepias. A major challenge to investigating coevolutionary hypotheses and conducting molecular ecology studies lies in the limited understanding of the evolutionary history and biogeographical patterns of Tetraopes. By integrating genomic, morphological, paleontological, and geographical data, we present a robust phylogeny of Tetraopes and their relatives, using three inference methods with varying subsets of data, encompassing 2-12 thousand UCE loci. We elucidate the diversification patterns of Tetraopes species across major biogeographical regions and their colonization of the American continent. Our findings suggest that the genus originated in Central America approximately 21 million years ago during the Miocene and diversified from the Mid-Miocene to the Pleistocene. These events coincided with intense geological activity in Central America. Additionally, independent colonization events in North America occurred from the Late Miocene to the early Pleistocene, potentially contributing to the early diversification of the group. Our data suggest that a common ancestor of Tetraopini migrated into North America, likely facilitated by North Atlantic land bridges, while closely related tribes diverged in Asia and Europe during the Paleocene. Establishing a robust and densely sampled phylogeny of Tetraopes beetles provides a foundation for investigating micro- and macroevolutionary phenomena, including clinal variation, coevolution, and detoxification mechanisms in this ecologically important group., (© 2024. The Author(s).)

The Neotropical social wasp genus Chartergus is reviewed and comparative diagnoses, images of type material, and an updated identification key for species are presented. To investigate the phylogenetic relationships within the genus, we used a combined dataset of morphological characters, nest architecture traits, and molecular data. The results revealed the following relationships among the Chartergus species: (C. globiventris + (C. artifex + C. metanotalis)).

Although MALDI-ToF platforms for microbial identifications have found great success in clinical microbiology, the sole use of protein fingerprints for the discrimination of closely related species, strain-level identifications, and detection of antimicrobial resistance remains a challenge for the technology. Several alternative mass spectrometry-based methods have been proposed to address the shortcomings of the protein-centric approach, including MALDI-ToF methods for fatty acid/lipid profiling and LC-MS profiling of metabolites. However, the molecular diversity of microbial pathogens suggests that no single "ome" will be sufficient for the accurate and sensitive identification of strain- and susceptibility-level profiling of bacteria. Here, we describe the development of an alternative approach to microorganism profiling that relies upon both metabolites and lipids rather than a single class of biomolecule. Single-phase extractions based on butanol, acetonitrile, and water (the BAW method) were evaluated for the recovery of lipids and metabolites from Gram-positive and -negative microorganisms. We found that BAW extraction solutions containing 45% butanol provided optimal recovery of both molecular classes in a single extraction. The single-phase extraction method was coupled to hydrophilic interaction liquid chromatography (HILIC) and ion mobility-mass spectrometry (IM-MS) to resolve similar-mass metabolites and lipids in three dimensions and provide multiple points of evidence for feature annotation in the absence of tandem mass spectrometry. We demonstrate that the combined use of metabolites and lipids can be used to differentiate microorganisms to the species- and strain-level for four of the ESKAPE pathogens ( Enterococcus faecium , Staphylococcus aureus , Acinetobacter baumannii , and Pseudomonas aeruginosa ) using data from a single ionization mode. These results present promising, early stage evidence for the use of multiomic signatures for the identification of microorganisms by liquid chromatography, ion mobility, and mass spectrometry that, upon further development, may improve upon the level of identification provided by current methods., Competing Interests: The authors declare no competing financial interest., (© 2023 The Authors. Published by American Chemical Society.)

Charterginus Fox, 1898 is a neotropical genus of swarm-founding social wasps consisting of six species. Despite its ecological significance, there are limited studies on various aspects of these wasps. To address this, this study aims to provide a comprehensive comparative diagnosis for all Charterginus species, accompanied by high-quality images of the type material. Moreover, a distribution map incorporating both previous and newly recorded occurrences is presented, shedding light on the geographical range of these species. Through these efforts, we aim to enhance the knowledge and facilitate future research on Charterginus, ultimately contributing to a better understanding of swarm-founding social wasps.

The genus Protopolybia Ducke was analyzed using a multilocus phylogenetic approach, which confirmed its monophyly. In contrast with the arrangements derived solely from morphological data, however, this analysis divides the genus into only two major clades. The study also presents divergence times for the origin and diversification of Protopolybia, which are related to possible vicariant events occurring in the Amazon biome.

A catalogue of Eumeninae occurring in Oceania is presented, the first in more than a century.

Fescue toxicosis is caused by grazing ergot alkaloid-producing endophyte ( Epichloë coenophiala )-infected tall fescue (E+). Summer grazing of E+ leads to decreased productivity, associated impaired thermoregulation, and altered behavior. The goal of this study was to determine the role of E+ grazing-climate interaction on animal behavior and thermoregulation during late fall. Eighteen Angus steers were placed on nontoxic (NT), toxic (E+) and endophyte-free (E-) fescue pastures for 28 days. Physiological parameters, such as rectal temperature (RT), respiration rate (RR), ear and ankle surface temperature (ET, AT), and body weights, were measured. Skin surface temperature (SST) and animal activity were recorded continuously with temperature and behavioral activity sensors, respectively. Environmental conditions were collected using paddocks-placed data loggers. Across the trial, steers on E+ gained about 60% less weight than the other two groups. E+ steers also had higher RT than E- and NT, and lower SST than NT post-pasture placement. Importantly, animals grazing E+ spent more time lying, less time standing, and took more steps. These data suggest that late fall E+ grazing impairs core and surface temperature regulation and increases non-productive lying time, which may be partly responsible for the observed decreased weight gains.

A taxonomic study on the Afrotropical species in the genus Anterhynchium de Saussure, 1863 is presented. Some subspecies are elevated to specific rank (Anterhynchium argenteopilosellum (Giordani Soika, 1937) stat. nov., A. bugandanum Giordani Soika, 1987 stat. nov., A. cariosum Giordani Soika, 1987 stat. nov., A. denticulatum (Mocsáry, 1903) stat. nov., A. sulphureomaculatum (von Schulthess, 1928) stat. nov.). New synonymies are proposed for Anterhynchium fallax (de Saussure, 1852) (=Rhynchium luctuosum Gerstäcker 1857 syn. nov.), Anterhynchium grandidieri (de Saussure, 1890) (= Epiodynerus grandidieri limbatulus Giordani Soika, 1991 syn. nov.), Anterhynchium grayi (de Saussure, 1855) (= Synagris inermis var. atrata Mocsáry, 1903 syn. nov.; = Rhynchium grayi var. sumptuosum Gribodo, 1895 syn. nov.), Anterhynchium mephisto (Gribodo, 1892) (= Anterhynchium beninum Gusenleitner, 2018 syn. nov.; = Odynerus rufoniger Bequaert, 1918 syn. nov.; = Synagris uncata Tullgren, 1904 syn. nov.); Anterhynchium synagroide (de Saussure, 1852) (=Rhynchium synagroides var. alpha von Schulthess, 1923 syn. nov.; Rhynchium synagroides var. beta von Schulthess, 1923 syn. nov.), Anterhynchium andreanum (de Saussure, 1890) (= Odynerus andreanus discolor Giordani Soika, 1941 syn. nov.); Anterhynchium synagroide gamma (von Schulthess, 1924) is considered as a doubtful synonym of A. argenteopilosellum syn. nov. The nominal subgenus is redefined and divided into the following species-groups: grayi species-group, mephisto species-group, osborni species-group and synagroide species-group. The Malagasy species are transferred from Epiodynerus Giordani Soika to the nominal subgenus but considered as incertae sedis in it. Odynerus natalensis de Saussure, 1855 and Anterhynchium uniforme Gusenleitner, 2012 are transferred to Antodynerus de Saussure, 1855 (Antodynerus natalensis comb. nov. and Antodynerus uniforme comb. nov.), Rhynchium histrionicum Gerstäcker, 1857 is transferred to Euodynerus Dalla Torre, 1904 (Euodynerus (Euodynerus) histrionicus comb. nov.), Rhynchium holomelas André, 1895 is transferred to Pseudagris de Saussure, 1863 (Pseudagris holomelas comb. nov.; = Rhynchium junodianum von Schulthess, 1899 syn. nov.; = Synagris aterrima Maidl, 1914 syn. nov.), Anterhynchium obscurum Gusenleitner, 2007 is transferred to Tricarinodynerus Giordani Soika, 1952 (Tricarinodynerus obscurus comb. nov.). Anterhynchium dahomeyicus Gusenleitner, 2018 is a junior synonym of Aethiopicodynerus punctiventris (Gusenleitner, 2002) syn. nov. Lectotypes for Odynerus andreanus de Saussure, 1890 and Rhynchium sulphureomaculatum von Schulthess, 1928 are designated. A key to the species is provided.

A catalogue of the social wasps occurring in Australia is presented. New synonymies include Vespa cohabitatrix Curtiss, 1938 (= Polistes olivaceus (DeGeer, 1773)) and Vespula vulgaris vetus Eck, 1999 (= Vespula vulgaris (Linnaeus, 1758)).

Chemical overexposures and war-related stress during the 1990-1991 Gulf War (GW) are implicated in the persisting pathological symptoms that many GW veterans continue to endure. These symptoms culminate into a disease known as Gulf War Illness (GWI) and affect about a third of the GW veteran population. Currently, comprehensive effective GWI treatment options are unavailable. Here, an established GWI mouse model was utilized to explore the (1) long-term behavioral and neuroinflammatory effects of deployment-related GWI chemicals exposure and (2) ability of the immunotherapeutic lacto-N-fucopentaose III (LNFPIII) to improve deficits when given months after the end of exposure. Male C57BL6/J mice (8-9 weeks old) were administered pyridostigmine bromide (PB) and DEET for 14 days along with corticosterone (CORT; latter 7 days) to emulate wartime stress. On day 15, a single injection of the nerve agent surrogate diisopropylfluorophosphate (DFP) was given. LNFPIII treatment began 7 months post GWI chemicals exposure and continued until study completion. A battery of behavioral tests for assessment of cognition/memory, mood, and motor function in rodents was performed beginning 8 months after exposure termination and was then followed by immunohistochemcal evaluation of neuroinflammation and neurogenesis. Within tests of motor function, prior GWI chemical exposure led to hyperactivity, impaired sensorimotor function, and altered gait. LNFPIII attenuated these motor-related deficits and improved overall grip strength. GWI mice also exhibited more anxiety-like behavior that was reduced by LNFPIII; this was test-specific. Short-term, but not long-term memory, was impaired by prior GWI exposure; LNFPIII improved this measure. In the brains of GWI mice, but not in mice treated with LNFPIII, glial activation was increased. Overall, it appears that months after exposure to GWI chemicals, behavioral deficits and neuroinflammation are present. Many of these deficits were attenuated by LNFPIII when treatment began long after GWI chemical exposure termination, highlighting its therapeutic potential for veterans with GWI., (© 2022 The Authors.)

A cladistic analysis of the genus Synagris is performed to assess the taxonomic status and limits of its subgenera, examining external morphology and male genitalia. The subgenera S. (Rhynchagris) and S. (Pseudagris) are raised to generic rank (Rhynchagris stat. nov. and Pseudagris stat. nov.), with the former as senior synonym of Pteromenes Giordani Soika syn. nov.; the subgenus S. (Hypagris) stat. res. is no longer a synonym of S. (Paragris); while S. (Synagris) is monophyletic and retains its status as subgenus. Replacement name due to homonymy is proposed for Synagris cornuta maculata Bequaert, 1918 (Synagris cornuta maculatoides nom. nov.).

The subfamily Eumeninae plays a significant role in the biological control of agricultural pests. However, the characteristics of eumenine mitogenomes that are important molecular markers for phylogenetics are not clearly revealed. Here, 52 eumenine mitogenomes are newly sequenced and annotated, and the phylogenetic relationships of the subfamily are comprehensively analyzed based on 87 vespid mitogenomes. Through the comparative analysis of the 54 eumenine mitogenomes, the gene compositions of about one half of the 54 species match with ancestral insect mitogenome, and remaining others contain two trnM which are highly similar, with 51.86% ( Eumenes tripunctatus ) to 90.65% ( Pseumenes nigripectus ) sequence identities, which is unique among the reported mitogenomes of the family Vespidae. Moreover, the translocation trnL1 upstream of nad1 is a common rearrangement event in all eumenine mitogenomes. The results of phylogenetic analyses support the paraphyly of the subfamily Eumeninae and the tribe Odynerini, respectively, and the monophyly of the tribe Eumenini, and verify that the tribe Zethini is a valid subfamily Zethinae. In this study, the relationships between some genera such as Allorhynchium and Pararrhynchium or the taxonomic status of the subgenera such as Eremodynerus and Dirhynchium are found to be confusing and there should be further inquiry with more samples.

An account of the Antonio Giordani Soika Eumeninae collection housed at the Natural History Museum of Venice Giancarlo Ligabue is presented, following its recent reorganization. A list of identified species, with the count of specimens, is provided, as well as details on type specimens. With over 21,000 identified specimens and 2,756 type specimens from all zoogeographical regions, the collection covers over 60% of the worlds Eumeninae species. Several incongruities emerged during the verification of type specimens. A brief history of the collection is presented, with comments on Giordani Soikas activity.

Bovine fescue toxicosis (FT) is caused by grazing ergot alkaloid-producing endophyte (Epichloë coenophiala)-infected tall fescue. Endophyte's effects on the animal's microbiota and metabolism were investigated recently, but its effects in planta or on the plant-animal interactions have not been considered. We examined multi-compartment microbiota-metabolome perturbations using multi-'omics (16S and ITS2 sequencing, plus untargeted metabolomics) in Angus steers grazing non-toxic (Max-Q) or toxic (E+) tall fescue for 28 days and in E+ plants. E+ altered the plant/animal microbiota, decreasing most ruminal fungi, with mixed effects on rumen bacteria and fecal microbiota. Metabolic perturbations occurred in all matrices, with some plant-animal overlap (e.g., Vitamin B6 metabolism). Integrative interactomics revealed unique E+ network constituents. Only E+ had ruminal solids OTUs within the network and fecal fungal OTUs in E+ had unique taxa (e.g., Anaeromyces). Three E+-unique urinary metabolites that could be potential biomarkers of FT and targeted therapeutically were identified., (© 2022. The Author(s).)

Sex and age have distinct influences and roles in behavior and immune reactivity; yet, most studies use adult male rodents with little attention to middle age, a time associated with key physiological transitions in both sexes. Thus, this study investigated sex differences during middle age in behavior, immune response to lipopolysaccharide (LPS), and glucose regulation in C57BL/6 mice with GFP-tagged monocytes/microglia. Behaviorally, males performed better in tests of motor function (Open Field [OF], Grip Strength, Sticker Removal, Gait, and Pole tests) and displayed less depressive- and anxiety-like behaviors across multiple mood tests (OF, Elevated Zero Maze, Sucrose Preference, and Swim test). However, females performed better in tests of cognition (Barnes Maze and Novel Object Recognition). Following behavioral assessment, mice were given LPS to characterize sex-dependent inflammagen responses. Females displayed greater sickness behavior in the OF, higher levels of peripheral cytokines, and subtle neuroinflammation in the cortex, striatum, and hippocampus. A separate middle-aged cohort was used for glucose tolerance and insulin sensitivity testing. Both sexes had excessive blood glucose rebound after insulin challenge, but displayed differences following glucose administration, where males had higher baseline glucose and females remained hyperglycemic. This study suggests that during middle-age male mice have better emotional regulation and motor function, but not cognitive ability than females. Further, males are less sensitive than females to the acute effects of LPS peripherally and centrally, but both sexes showed sex-specific impairments in blood glucose regulation. Overall, it appears that middle age is an important transition point with multiple sex differences, some of which are unique to this stage of life., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Up to 80% of the fatty acids in Staphylococcus aureus membrane lipids are branched, rather than straight-chain, fatty acids. The branched fatty acids (BCFAs) may have either an even or odd number of carbons, and the branch position may be at the penultimate carbon ( iso ) or the antepenultimate ( anteiso ) carbon of the tail. This results in two sets of isomeric fatty acid species with the same number of carbons that cannot be resolved by mass spectrometry. The isomer/isobar challenge is further complicated when the mixture of BCFAs and straight-chain fatty acids (SCFAs) are esterified into diacylated lipids such as the phosphatidylglycerol (PG) species of the S. aureus membrane. No conventional chromatographic method has been able to resolve diacylated lipids containing mixtures of SCFAs, anteiso -odd, iso -odd, and iso -even BCFAs. A major hurdle to method development in this area is the lack of relevant analytical standards for lipids containing BCFA isomers. The diversity of the S. aureus lipidome and its naturally high levels of BCFAs present an opportunity to explore the potential of resolving diacylated lipids containing BCFAs and SFCAs. Using our knowledge of lipid and fatty acid biosynthesis in S. aureus , we have used a stable-isotope-labeling strategy to develop and validate a 30 min C18 reversed-phase liquid chromatography method combined with traveling-wave ion mobility-mass spectrometry to provide resolution of diacylated lipids based on the number of BCFAs that they contain.

Residual effects of the 1990-1991 Gulf War (GW) still plague veterans 30 years later as Gulf War Illness (GWI). Thought to stem mostly from deployment-related chemical overexposures, GWI is a disease with multiple neurological symptoms with likely immunological underpinnings. Currently, GWI remains untreatable, and the long-term neurological disease manifestation is not characterized fully. The present study sought to expand and evaluate the long-term implications of prior GW chemicals exposure on neurological function 6-8 months post GWI-like symptomatology induction. Additionally, the beneficial effects of delayed treatment with the glycan immunotherapeutic lacto-N-fucopentaose III (LNFPIII) were evaluated. Male C57BL/6J mice underwent a 10-day combinational exposure (i.p.) to GW chemicals, the nerve agent prophylactic pyridostigmine bromide (PB) and the insecticide permethrin (PM; 0.7 and 200 mg/kg, respectively). Beginning 4 months after PB/PM exposure, a subset of the mice were treated twice a week until study completion with LNFPIII. Evaluation of cognition/memory, motor function, and mood was performed beginning 1 month after LNFPIII treatment initiation. Prior exposure to PB/PM produced multiple locomotor, neuromuscular, and sensorimotor deficits across several motor tests. Subtle anxiety-like behavior was also present in PB/PM mice in mood tests. Further, PB/PM-exposed mice learned at a slower rate, mostly during early phases of the learning and memory tests employed. LNFPIII treatment restored or improved many of these behaviors, particularly in motor and cognition/memory domains. Electrophysiology data collected from hippocampal slices 8 months post PB/PM exposure revealed modest aberrations in basal synaptic transmission and long-term potentiation in the dorsal or ventral hippocampus that were improved by LNFPIII treatment. Immunohistochemical analysis of tyrosine hydroxylase (TH), a dopaminergic marker, did not detect major PB/PM effects along the nigrostriatal pathway, but LNFPIII increased striatal TH. Additionally, neuroinflammatory cells were increased in PB/PM mice, an effect reduced by LNFPIII. Collectively, long-term neurobehavioral and neurobiological dysfunction associated with prior PB/PM exposure was characterized; delayed LNFPIII treatment provided multiple behavioral and biological beneficial effects in the context of GWI, highlighting its potential as a GWI therapeutic., (Copyright © 2021 Elsevier Inc. All rights reserved.)

Approximately one-third of Persian Gulf War veterans are afflicted by Gulf War Illness (GWI), a chronic multisymptom condition that fundamentally presents with cognitive deficits (i.e., learning and memory impairments) and neuroimmune dysfunction (i.e., inflammation). Factors associated with GWI include overexposures to neurotoxic pesticides and nerve agent prophylactics such as permethrin (PM) and pyridostigmine bromide (PB), respectively. GWI-related neurological impairments associated with PB-PM overexposures have been recapitulated in animal models; however, there is a paucity of studies assessing PB-PM-related aberrations in hippocampal synaptic plasticity and transmission that may underlie behavioral impairments. Importantly, FDA-approved neuroactive treatments are currently unavailable for GWI. In the present study, we assessed the efficacy of an immunomodulatory therapeutic, lacto-N-fucopentaose-III (LNFPIII), on ameliorating acute effects of in vivo PB-PM exposure on synaptic plasticity and transmission as well as trophic factor/cytokine expression along the hippocampal dorsoventral axis. PB-PM exposure resulted in hippocampal synaptic transmission deficits 48 h post-exposure, a response that was ameliorated by LNFPIII coadministration, particularly in the dorsal hippocampus (dH). LNFPIII coadministration also enhanced synaptic transmission in the dH and the ventral hippocampus (vH). Notably, LNFPIII coadministration elevated long-term potentiation in the dH. Further, PB-PM exposure and LNFPIII coadministration uniquely altered key inflammatory cytokine and trophic factor production in the dH and the vH. Collectively, these findings demonstrate that PB-PM exposure impaired hippocampal synaptic responses 48 h post-exposure, impairments that differentially manifested along the dorsoventral axis. Importantly, LNFPIII ameliorated GWI-related electrophysiological deficits, a beneficial effect indicating the potential efficacy of LNFPIII for treating GWI., (Copyright © 2021. Published by Elsevier B.V.)

Aims: The present study investigated if treatment with the immunotherapeutic, lacto-N-fucopentaose-III (LNFPIII), resulted in amelioration of acute and persisting deficits in synaptic plasticity and transmission as well as trophic factor expression along the hippocampal dorsoventral axis in a mouse model of Gulf War Illness (GWI)., Main Methods: Mice received either coadministered or delayed LNFPIII treatment throughout or following, respectively, exposure to a 15-day GWI induction paradigm. Subsets of animals were subsequently sacrificed 48 h, seven months, or 11 months post GWI-related (GWIR) exposure for hippocampal qPCR or in vitro electrophysiology experiments., Key Findings: Progressively worsened impairments in hippocampal synaptic plasticity, as well as a biphasic effect on hippocampal synaptic transmission, were detected in GWIR-exposed animals. Dorsoventral-specific impairments in hippocampal synaptic responses became more pronounced over time, particularly in the dorsal hippocampus. Notably, delayed LNFPIII treatment ameliorated GWI-related aberrations in hippocampal synaptic plasticity and transmission seven and 11 months post-exposure, an effect that was consistent with enhanced hippocampal trophic factor expression and absence of increased interleukin 6 (IL-6) in animals treated with LNFPIII., Significance: Approximately a third of Gulf War Veterans have GWI; however, GWI therapeutics are presently limited to targeted and symptomatic treatments. As increasing evidence underscores the substantial role of persisting neuroimmune dysfunction in GWI, efficacious neuroactive immunotherapeutics hold substantial promise in yielding GWI remission. The findings in the present report indicate that LNFPIII may be an efficacious candidate for ameliorating persisting neurological abnormalities presented in GWI., (Copyright © 2021 Elsevier Inc. All rights reserved.)

Neotropical swarm-founding wasps are divided into 19 genera in the tribe Epiponini (Vespidae, Polistinae). They display extensive variation in several colony-level traits that make them an attractive model system for reconstructing the evolution of social phenotypes, including caste dimorphism and nest architecture. Epiponini has been upheld as a solid monophyletic group in most phylogenetic analyses carried out so far, supported by molecular, morphological and behavioural data. Recent molecular studies, however, propose different relationships among the genera of swarm-founding wasps. This study is based on the most comprehensive epiponine sampling so far and was analyzed by combining morphological, nesting and molecular data. The resulting phylogenetic hypothesis shows many of the traditional clades but still impacts the way certain behavioural characters, such as nest structure and castes, evolved, and thus requires some re-interpretations. Angiopolybia as sister to the remaining Epiponini implies that nest envelopes and a casteless system are plesiomorphic in the tribe. Molecular dating points to an early tribal diversification during the Eocene (c. 55-38 Ma), with the major differentiation of current genera concentrated in the Oligocene/Miocene boundary., (© The Willi Hennig Society 2020.)

A catalogue of Eumeninae occurring in Australia is presented. The genus Lissodynerus Giordani Soika is synonymized with Pararrhynchium de Saussure. Species placed in the genus Pseudepipona de Saussure, by Giordani Soika and Borsato are transferred to Euodynerus Dalla Torre, and species placed in the genus Syneuodynerus Blüthgen, by Giordani Soika are transferred to Stenodyneriellus Giordani Soika. New combinations are Anterhynchium decoratum (de Saussure), A. tamarinum inviolatum (Giordani Soika), Australodynerus flavoniger (Giordani Soika), Deuterodiscoelius confusus (Giordani Soika), D. ephippium (de Saussure), D. insignis (de Saussure), D. spinosus (de Saussure), Euodynerus aborigenus (Borsato), E. alaris (de Saussure), E. angulatus alexandriae (Giordani Soika), E. angulatus angulatus (de Saussure), E. aspra (Giordani Soika), E. clypalaris (Giordani Soika), E. flavus (Giordani Soika), E. pallidus (Giordani Soika), E. succinctus purgatus(Giordani Soika), E. succinctus succinctus (de Saussure), Pararrhynchium desaussurei (Borsato), P. kurandensis (Giordani Soika), Pseudalastor aurantiacus (Borsato), P. pecularis (Borsato), Stenodyneriellus aurantiacus (Giordani Soika), S. aurantiopilosella (Giordani Soika), S. cylindrellus (Giordani Soika), S. longebispinosa (Giordani Soika) and S. occidentatus (Giordani Soika). Revised combinations are Australodynerus yanchepensis nigrithorax Giordani Soika and A. yanchepensis yanchepensis Giordani Soika, which are returned to that genus. Other new synonymies are Anterhynchium tamarinum septentrionale Giordani Soika = Anterhynchium tamarinum tamarinum (de Saussure), Ischnocoelia robusta analis Giordani Soika = I. robusta robusta (Meade-Waldo), Pseudabispa bicolor flavescentula (Giordani Soika) = P bicolor bicolor (de Saussure), Pseudalastor tridentatus septentrionalis Giordani Soika = P. tridentatus tridentatus Giordani Soika and Rhynchium australense Perkins = R. magnificum Smith.

Background: Physiological homeostasis decline, immunosenescence, and increased risk for multiple diseases, including neurodegeneration, are all hallmarks of ageing. Importantly, it is known that the ageing process is sex-biased. For example, there are sex differences in predisposition for multiple age-related diseases, including neurodegenerative and autoimmune diseases. However, sex differences in age-associated immune phenotypes are not clearly understood., Results: Here, we examined the effects of age on immune cell phenotypes in both sexes of C57BL/6J mice with a particular focus on NK cells. We found female-specific spleen weight increases with age and concordant reduction in the number of splenocytes per gram of spleen weight compared to young females. To evaluate sex- and age-associated changes in splenic immune cell composition, we performed flow cytometry analysis. In male mice, we observed an age-associated reduction in the frequencies of monocytes and NK cells; female mice displayed a reduction in B cells, NK cells, and CD8 + T cells and increased frequency of monocytes and neutrophils with age. We then performed a whole blood stimulation assay and multiplex analyses of plasma cytokines and observed age- and sex-specific differences in immune cell reactivity and basal circulating cytokine concentrations. As we have previously illustrated a potential role of NK cells in Parkinson's disease, an age-related neurodegenerative disease, we further analyzed age-associated changes in NK cell phenotypes and function. There were distinct differences between the sexes in age-associated changes in the expression of NK cell receptors, IFN-γ production, and impairment of α-synuclein endocytosis., Conclusions: This study demonstrates sex- and age-specific alterations in splenic lymphocyte composition, circulating cytokine/chemokine profiles, and NK cell phenotype and effector functions. Our data provide evidence that age-related physiological perturbations differ between the sexes which may help elucidate sex differences in age-related diseases, including neurodegenerative diseases, particularly Parkinson's disease, where immune dysfunction is implicated in their etiology.

A taxonomic revision of the Protopolybia chartergoides species-group was undertaken. Protopolybia isthmensis (Richards) stat. nov. is elevated to the level of species. The male genitalia of P. chartergoides (Gribodo), P. fuscata (Fox), and P. pallidibalteatus (Cameron) are illustrated and described. New collection records and new identification keys are presented for the species groups of Protopolybia, and the species within the P. chartergoides species-group.

The microbiota's influence on host (patho) physiology has gained interest in the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut-brain-immune axis. This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model. Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total). Fecal 16S rRNA sequencing was performed on all samples post-LNFPIII treatment to assess microbiota effects of GWI chemicals and acute/delayed LNFPIII administration. Although PB/PM did not affect species composition on a global scale, it affected specific taxa in both short- and long-term settings. PB/PM elicited more prominent long-term effects, notably, on the abundances of bacteria belonging to Lachnospiraceae and Ruminococcaceae families and the genus Allobaculum . LNFPIII improved a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus , Ruminococcous ) in PB/PM-treated mice, indicating a positive selection pressure for these bacteria. Multiple operational taxonomic units correlated with aberrant behavior and lipocalin-2 in PB/PM samples; LNFPIII was modulatory. Overall, significant and lasting GWI effects occurred on specific microbiota and LNFPIII treatment was beneficial.

This study presents a checklist of the vespid wasps (Hymenoptera: Vespidae) of India. A total of 288 species belonging to 60 genera and 5 subfamilies of Vespidae are known to occur within the political boundaries of India. A complete list of species, comprising valid scientific names, synonyms, geographical distribution within and outside India, along with references, is provided. One new synonymy is proposed.

An updated checklist of the Arabian species of Vespidae is provided here. The list includes 163 identified species in 49 genera and four subfamilies: Eumeninae (41 genera, 130 species), Masarinae (4 genera, 14 species), Polistinae (3 genera, 17 species) and Vespinae (1 genus, 2 species). Distributions, both on the Peninsula and extra-limital, are provided for each species and subspecies; 53 species (33%) are endemic to the Arabian Peninsula, as are 15 subspecies. A new combination is proposed: Knemodynerus sauditus (Giordani Soika, 1989) comb. nov., transferred from Trachyodynerus Giordani Soika, 1989.

Gulf War Illness (GWI) manifests a multitude of symptoms, including neurological and immunological, and approximately a third of the 1990-1991 Gulf War (GW) veterans suffer from it. This study sought to characterize the acute neurochemical (monoamine) and neuroinflammatory profiles of two established GWI animal models and examine the potential modulatory effects of the novel immunotherapeutic Lacto-N-fucopentaose III (LNFPIII). In Model 1, male C57BL/6 J mice were treated for 10 days with pyridostigmine bromide (PB) and permethrin (PM). In Model 2, a separate cohort of mice were treated for 14 days with PB and N,N-Diethyl-methylbenzamide (DEET), plus corticosterone (CORT) via drinking water on days 8-14 and diisopropylfluorophosphate (DFP) on day 15. LNFPIII was administered concurrently with GWI chemicals treatments. Brain and spleen monoamines and hippocampal inflammatory marker expression were examined by, respectively, HPLC-ECD and qPCR, 6 h post treatment cessation. Serotonergic (5-HT) and dopaminergic (DA) dyshomeostasis caused by GWI chemicals was apparent in multiple brain regions, primarily in the nucleus accumbens (5-HT) and hippocampus (5-HT, DA) for both models. Splenic levels of 5-HT (both models) and norepinephrine (Model 2) were also disrupted by GWI chemicals. LNFPIII treatment prevented many of the GWI chemicals induced monoamine alterations. Hippocampal inflammatory cytokines were increased in both models, but the magnitude and spread of inflammation was greater in Model 2; LNFPIII was anti-inflammatory, more so in the apparently milder Model 1. Overall, in both models, GWI chemicals led to monoamine disbalance and neuroinflammation. LNFPIII co-treatment prevented many of these disruptions in both models, which is indicative of its promise as a potential GWI therapeutic., Competing Interests: Declaration of Competing Interest The authors declare no conflicts of interest., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Individuals frequently choose between accomplishing goals using unaided cognitive abilities or offloading cognitive demands onto external tools and resources. For example, in order to remember an upcoming appointment one might rely on unaided memory or create a reminder by setting a smartphone alert. Setting a reminder incurs both a cost (the time/effort to set it up) and a benefit (increased likelihood of remembering). Here we investigate whether individuals weigh such costs/benefits optimally or show systematic biases. In 3 experiments, participants performed a memory task where they could choose between (a) earning a maximum reward for each remembered item, using unaided memory; or (b) earning a lesser amount per item, using external reminders to increase the number remembered. Participants were significantly biased toward using external reminders, even when they had a financial incentive to choose optimally. Individual differences in this bias were stable over time, and predicted by participants' erroneous metacognitive underconfidence in their memory abilities. Bias was eliminated, however, when participants received metacognitive advice about which strategy was likely to maximize performance. Furthermore, we found that metacognitive interventions (manipulation of feedback valence and practice-trial difficulty) yielded shifts in participants' reminder bias that were mediated by shifts in confidence. However, the bias could not be fully attributed to metacognitive error. We conclude that individuals have stable biases toward using external versus internal cognitive resources, which result at least in part from inaccurate metacognitive evaluations. Finding interventions to mitigate these biases can improve individuals' adaptive use of cognitive tools. (PsycINFO Database Record (c) 2020 APA, all rights reserved).

At low oxygen concentrations, respiration of Pseudomonas aeruginosa (Pa) and other bacteria relies on activity of cytochrome cbb 3 oxidases. A diheme cytochrome c 4 (cyt c 4 ) donates electrons to Pa cbb 3 oxidases to enable oxygen reduction and proton pumping by these enzymes. Given the importance of this redox pathway for bacterial pathogenesis, both cyt c 4 and cbb 3 oxidase are potential targets for new antibacterial strategies. The structural information about these two proteins, however, is scarce, and functional insights for Pa and other bacteria have been primarily drawn from analyses of the analogous system from Pseudomonas stutzeri (Ps). Herein, we describe characterization of structural and redox properties of cyt c 4 from Pa. The crystal structure of Pa cyt c 4 has revealed that this protein is organized in two monoheme domains. The interdomain interface is more hydrophobic in Pa cyt c 4 , and the protein surface does not show the dipolar distribution of charges found in Ps cyt c 4 . The reduction potentials of the two hemes are similar in Pa cyt c 4 but differ by about 100 mV in Ps cyt c 4 . Analyses of structural models of these and other cyt c 4 proteins suggest that multiple factors contribute to the potential difference of the two hemes in these proteins, including solvent accessibility of the heme group, the distribution of surface charges, and the nature of the interdomain interface. The distinct properties of cyt c 4 proteins from closely-related Pa and Ps bacteria emphasize the importance of examining the cbb 3 /cyt c 4 redox pathway in multiple species., (Copyright © 2019. Published by Elsevier Inc.)

A taxonomic revision of the Protopolybia picteti-emortualis species-group now demonstrates that this group is possibly the largest in Protopolybia, comprising 15 species: P. eldinaris sp. nov.; P. djaneteae sp. nov.; P. cameranii (Zavattari 1906) = P. bella (Von Ihering 1903) syn. nov.; P. fulvotincta stat. rev.; P. biguttata, P. nitida, P. alvarengai, P. iheringi, P. perfulvula, P. steinbachi, P. wheeleri, P. rotundata, P. picteti, P. emortualis and P. duckei. Male and female genitalia are illustrated for P. biguttata Bequaert, P. bella, P. nitida, P. fulvotincta and P. picteti. New collection records and a new identification key are presented for this species-group.

The genus Gastrodynerus is here revised based on external morphology and male genitalia. Bohart (1984) proposed Gastrodynerus as a new genus with three species: G. vanduzeei (Bohart 1948), G. searsi Bohart, 1984, and G. stangei Bohart, 1984. Later, Rodriguez-Palafox (1996) added G. tacubayae (de Saussure, 1857). Herein, we describe four new species from Central America and South America: G. guatemalensis sp. nov.; G. barretti sp. nov.; G. aimara sp. nov. and G. yungaensis sp. nov. After this taxonomic revision, the Gastrodynerus includes eight species widely distributed in the New World. An identification key is provided for all of the species.

In this study, the mitochondrial genomes of three Stenogastrinae species, Eustenogaster scitula, Liostenogaster nitidipennis and Parishnogaster mellyi were sequenced and annotated, and a total of 16 vespid mtgenomes are comparatively analyzed. Our results indicate that codon usage bias is mainly dominated by mutational pressure, and affected only slightly by natural selection. Selective pressure analysis of protein-coding genes (PCGs) shows that the highest evolutionary rate is present in NADH complex I, and the lowest in cox1. Compared with the reported mtgenomes of other Vespidae, in Stenogastrinae, trnH is shifted to a new position. Phylogenetic analyses are performed using Bayesian method and Maximum Parsimony. Phylogenetic analysis further confirms that the Stenogastrinae is the sister group of all remaining Vespidae. Divergence time of Stenogastrinae from other Vespidae is estimated at ~ 166 Mya. Our results also support that eusociality evolved twice in the family Vespidae., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Eight new species, namely Parancistrocerus latitergus sp. nov. (China), P. lamnulus sp. nov. (China), P. laticlypeus sp. nov. (Thailand), P. prominens sp. nov. (Thailand), P. guangxiensis sp. nov. (China), P. discarinatus sp. nov. (Thailand), P. abyssicavus sp. nov. (Thailand), and P. similiandrocles sp. nov. (China) are described and illustrated. Parancistrocerus samarensis (von Schulthess, 1934) and P. incorruptus incorruptus Giordani Soika, 1972, are newly recorded from China and Thailand, respectively. An updated key to the Oriental species of the genus Parancistrocerus is also given.

Three new species, namely Stenodyneriellusangustus sp. n. from Thailand, S.profundus sp. n. from Philippines, and S.longitergus sp. n. from Indonesia, are described and illustrated in detail. Stenodyneriellusmaculatus Gusenleitner, 2013 is newly recorded from Vietnam. Two keys to some related species are also provided.

The genus Orientalicesa has six species, and all of them have been recorded in the Oriental region (including Indonesia, Malaysia, Phillippines, Laos, China). In this paper, the genus Orientalicesa is newly recorded from Vietnam, represented by one species O. confasciatus Tan and Carpenter. This species was mistakenly redescribed as Stenodyneriellus rangpocus Kumar, Carpenter Kishore, 2017. A new synonym of O. confasciatus is proposed, and that species is a new record for India.

Neutron crystallography is a powerful technique for directly visualizing the locations of H atoms in biological macromolecules. This information has provided key new insights into enzyme mechanisms, ligand binding and hydration. However, despite the importance of this information, the application of neutron crystallography in biology has been limited by the relatively low flux of available neutron beams and the large incoherent neutron scattering from hydrogen, both of which contribute to weak diffraction data with relatively low signal-to-background ratios. A method has been developed to fit weak data based on three-dimensional profile fitting of Bragg peaks in reciprocal space by an Ikeda-Carpenter function with a bivariate Gaussian. When applied to data collected from three different proteins, three-dimensional profile fitting yields intensities with higher correlation coefficients (CC 1/2 ) at high resolutions, decreased R free factors, extended resolutions and improved nuclear density maps. Importantly, additional features are revealed in nuclear density maps that may provide additional scientific information. These results suggest that three-dimensional profile fitting will help to extend the capabilities of neutron macromolecular crystallography., (open access.)

The male genitalia of 58 species of the New World Polistes are described: 29 of Polistes (Aphanilopterus), 12 of Polistes (Epicnemius), 13 of Polistes (Fuscopolistes), one of Polistes (Onerarius) and three of Polistes (Palisotius). Comments and remarks are also provided.

The hypothesis that eusociality originated once in Vespidae has shaped interpretation of social evolution for decades and has driven the supposition that preimaginal morphophysiological differences between castes were absent at the outset of eusociality. Many researchers also consider casteless nest-sharing an antecedent to eusociality. Together, these ideas endorse a stepwise progression of social evolution in wasps (solitary → casteless nest-sharing → eusociality with rudimentary behavioral castes → eusociality with preimaginal caste-biasing (PCB) → morphologically differentiated castes). Here, we infer the phylogeny of Vespidae using sequence data generated via anchored hybrid enrichment from 378 loci across 136 vespid species and perform ancestral state reconstructions to test whether rudimentary and monomorphic castes characterized the initial stages of eusocial evolution. Our results reject the single origin of eusociality hypothesis, contest the supposition that eusociality emerged from a casteless nest-sharing ancestor, and suggest that eusociality in Polistinae + Vespinae began with castes having morphological differences. An abrupt appearance of castes with ontogenetically established morphophysiological differences conflicts with the current conception of stepwise social evolution and suggests that the climb up the ladder of sociality does not occur through sequential mutation. Phenotypic plasticity and standing genetic variation could explain how cooperative brood care evolved in concert with nest-sharing and how morphologically dissimilar castes arose without a rudimentary intermediate. Furthermore, PCB at the outset of eusociality implicates a subsocial route to eusociality in Polistinae + Vespinae, emphasizing the role of mother-daughter interactions and subfertility (i.e. the cost component of kin selection) in the origin of workers., (© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.)

The Oriental species of Allorhynchium van der Vecht, 1963 (Hymenoptera: Vespidae: Eumeninae) are keyed for the first time and their distribution is summarised, with special reference to China and Vietnam. Archancistrocerus Giordani Soika, 1986, is synonymized with Allorhynchium van der Vecht (syn. nov.). Allorhynchium diffinis (Giordani Soika, 1986) is a new combination and A. quadrimaculatum Gusenleitner, 1997, is recorded from Vietnam for the first time.

[This corrects the article DOI: 10.3897/zookeys.740.22654.].