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3. Cruciferous Weed Isolates of Xanthomonas campestris Yield Insight into Pathovar Genomic Relationships and Genetic Determinants of Host and Tissue Specificity

Author

Dubrow, Zoë E., primary, Carpenter, Sara C. D., additional, Carter, Morgan E., additional, Grinage, Ayress, additional, Gris, Carine, additional, Lauber, Emmanuelle, additional, Butchachas, Jules, additional, Jacobs, Jonathan M., additional, Smart, Christine D., additional, Tancos, Matthew A., additional, Noël, Laurent D., additional, and Bogdanove, Adam J., additional

Published
2022
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6. Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants

Author

Wang, Li, primary, Kaya, Hilal Betul, additional, Zhang, Ning, additional, Rai, Rhitu, additional, Willmann, Matthew R., additional, Carpenter, Sara C. D., additional, Read, Andrew C., additional, Martin, Federico, additional, Fei, Zhangjun, additional, Leach, Jan E., additional, Martin, Gregory B., additional, and Bogdanove, Adam J., additional

Published
2021
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7. An xa5 Resistance Gene-Breaking Indian Strain of the Rice Bacterial Blight Pathogen Xanthomonas oryzae pv. oryzae Is Nearly Identical to a Thai Strain

Author

Carpenter, Sara C. D., primary, Mishra, Prashant, additional, Ghoshal, Chandrika, additional, Dash, Prasanta K., additional, Wang, Li, additional, Midha, Samriti, additional, Laha, Gouri S., additional, Lore, Jagjeet S., additional, Kositratana, Wichai, additional, Singh, Nagendra K., additional, Singh, Kuldeep, additional, Patil, Prabhu B., additional, Oliva, Ricardo, additional, Patarapuwadol, Sujin, additional, Bogdanove, Adam J., additional, and Rai, Rhitu, additional

Published
2020
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8. Spelling changes and fluorescent tagging with prime editing vectors for plants

Author

Wang, Li, primary, Kaya, Hilal Betul, additional, Zhang, Ning, additional, Rai, Rhitu, additional, Willmann, Matthew R., additional, Carpenter, Sara C. D., additional, Read, Andrew C., additional, Martin, Federico, additional, Fei, Zhangjun, additional, Leach, Jan E., additional, Martin, Gregory B., additional, and Bogdanove, Adam J., additional

Published
2020
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10. A TAL effector-like protein of an endofungal bacterium increases the stress tolerance and alters the transcriptome of the host.

Author

Carter, Morgan E., Carpenter, Sara C. D., Dubrow, Zoë E., Sabol, Mark R., Rinaldi, Fabio C., Lastovetsky, Olga A., Mondo, Stephen J., Pawlowska, Teresa E., and Bogdanove, Adam J.

Subjects
*BACTERIAL proteins, *PHYTOPATHOGENIC bacteria, *DNA-binding proteins, *BACTERIAL typing, *GENE knockout
Abstract

Symbioses of bacteria with fungi have only recently been described and are poorly understood. In the symbiosis of Mycetohabitans (formerly Burkholderia) rhizoxinica with the fungus Rhizopus microsporus, bacterial type III (T3) secretion is known to be essential. Proteins resembling T3-secreted transcription activator-like (TAL) effectors of plant pathogenic bacteria are encoded in the three sequenced Mycetohabitans spp. genomes. TAL effectors nuclearlocalize in plants, where they bind and activate genes important in disease. The Burkholderia TAL-like (Btl) proteins bind DNA but lack the N- and C-terminal regions, in which TAL effectors harbor their T3 and nuclear localization signals, and activation domain. We characterized a Btl protein, Btl19-13, and found that, despite the structural differences, it can be T3-secreted and can nuclearlocalize. A btl19-13 gene knockout did not prevent the bacterium from infecting the fungus, but the fungus became less tolerant to cell membrane stress. Btl19-13 did not alter transcription in a plantbased reporter assay, but 15 R. microsporus genes were differentially expressed in comparisons both of the fungus infected with the wild-type bacterium vs. the mutant and with the mutant vs. a complemented strain. Southern blotting revealed btl genes in 14 diverse Mycetohabitans isolates. However, banding patterns and available sequences suggest variation, and the btl19-13 phenotype could not be rescued by a btl gene from a different strain. Our findings support the conclusion that Btl proteins are effectors that act on host DNA and play important but varied or possibly host genotypespecific roles in the M. rhizoxinica-R. microsporus symbiosis. [ABSTRACT FROM AUTHOR]

Published
2020
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11. A Strain of an Emerging Indian Xanthomonas oryzae pv. oryzae Pathotype Defeats the Rice Bacterial Blight Resistance Gene xa13 Without Inducing a Clade III SWEET Gene and Is Nearly Identical to a Recent Thai Isolate

Author

Carpenter, Sara C. D., primary, Mishra, Prashant, additional, Ghoshal, Chandrika, additional, Dash, Prasanta K., additional, Wang, Li, additional, Midha, Samriti, additional, Laha, Gouri S., additional, Lore, Jagjeet S., additional, Kositratana, Wichai, additional, Singh, Nagendra K., additional, Singh, Kuldeep, additional, Patil, Prabhu B., additional, Oliva, Ricardo, additional, Patarapuwadol, Sujin, additional, Bogdanove, Adam J., additional, and Rai, Rhitu, additional

Published
2018
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12. A strain of an emerging Indian pathotype of Xanthomonas oryzae pv. oryzae defeats the rice bacterial blight resistance gene xa13 without inducing a clade III SWEET gene and is nearly identical to a recent Thai isolate

Author

Carpenter, Sara C. D., primary, Mishra, Prashant, additional, Ghoshal, Chandrika, additional, Dash, Prasanta, additional, Wang, Li, additional, Midha, Samriti, additional, Laha, Gouri S., additional, Lore, Jagjeet S, additional, Kositratana, Wichai, additional, Singh, Nagendra K., additional, Singh, Kuldeep, additional, Patil, Prabhu B., additional, Oliva, Ricardo, additional, Patarapuwadol, Sujin, additional, Bogdanove, Adam J, additional, and Rai, Rhitu, additional

Published
2018
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13. Functional analysis of African Xanthomonas oryzae pv. oryzae TALomes reveals a new susceptibility gene in bacterial leaf blight of rice

Author

Tran, Tuan T., primary, Pérez-Quintero, Alvaro L., additional, Wonni, Issa, additional, Carpenter, Sara C. D., additional, Yu, Yanhua, additional, Wang, Li, additional, Leach, Jan E., additional, Verdier, Valérie, additional, Cunnac, Sébastien, additional, Bogdanove, Adam J., additional, Koebnik, Ralf, additional, Hutin, Mathilde, additional, and Szurek, Boris, additional

Published
2018
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14. Functional analysis of African Xanthomonas oryzae pv. oryzae TALomes reveals a new susceptibility gene in bacterial leaf blight of rice

Author

Tran, Tuan Tu, primary, Pérez-Quintero, Alvaro L, additional, Wonni, Issa, additional, Carpenter, Sara C. D., additional, Yu, Yanhua, additional, Wang, Li, additional, Leach, Jan E., additional, Verdier, Valérie, additional, Cunnac, Sébastien, additional, Bogdanove, Adam J., additional, Koebnik, Ralf, additional, Hutin, Mathilde, additional, and Szurek, Boris, additional

Published
2018
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15. TAL effector driven induction of a SWEET gene confers susceptibility to bacterial blight of cotton

Author

Cox, Kevin L., primary, Meng, Fanhong, additional, Wilkins, Katherine E., additional, Li, Fangjun, additional, Wang, Ping, additional, Booher, Nicholas J., additional, Carpenter, Sara C. D., additional, Chen, Li-Qing, additional, Zheng, Hui, additional, Gao, Xiquan, additional, Zheng, Yi, additional, Fei, Zhangjun, additional, Yu, John Z., additional, Isakeit, Thomas, additional, Wheeler, Terry, additional, Frommer, Wolf B., additional, He, Ping, additional, Bogdanove, Adam J., additional, and Shan, Libo, additional

Published
2017
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20. Evaluation of Onion (Allium cepa L.) Lines and Hybrids Possessing Bs1for Resistance against Multiple Isolates of Botrytis squamosa

Author

Hyde, Peter T., Leckie, Brian M., Carpenter, Sara C. D., Lorbeer, James W., Hoepting, Christine A., Ullrich, Maire R., and Mutschler, Martha A.

Abstract

Onion (Allium cepaL.) lines resistant to Botrytisleaf blight (BLB) were produced by backcrossing the Bs1BLB resistance of Allium royleiStearn into cultivated onion. This study evaluated important issues for the commercial use of BLB‐resistant onions: the relative degree of BLB control possible when Bs1is homozygous rather than heterozygous, the control achieved against multiple pathogen isolates, and the potential for negative traits associated with transfer of Bs1. Homozygous BLB‐resistant onions tested in inoculated growth chamber screens with five distinct Botrytis squamosaWalker isolates and in naturally infested regional field trials showed significantly lower BLB symptoms than susceptible commercial hybrid controls. F1hybrids heterozygous for the Bs1gene showed levels of BLB symptoms between those of the homozygous resistant and susceptible controls, confirming previous reports of partial dominance. The difference in BLB control in plants heterozygous for Bs1compared with homozygous plants was more noticeable under heavier disease pressure. Hybrids heterozygous for Bs1had similar level of BLB control in most of the field trials as their parental homozygous BLB‐resistant lines, indicating that either heterozygous or homozygous BLB resistant hybrids could be used commercially for BLB control. There were no differences in the degree of pathogenicity across the five B. squamosaisolates, which were genetically different based on polymorphism for DNA markers, or among the naturally occurring pathogens present at the six different field locations used. No obvious association was noted between presence of the Bs1resistance gene with unfavorable characteristics such as reduced bulb size or yield.

Published
2015
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21. Secretion and translocation signals and DspB/F- binding domains in the type Ill effector DspA/E of Erwinia amylovora.

Author

Chang-Sik Oh, Carpenter, Sara C. D., Hayes, Marshall L., and Beer, Steven V.

Subjects
*PLANT translocation, *REGULATION of secretion, *PHYSIOLOGICAL control systems, *BACTERIAL proteins, *PLANT plasma membranes
Abstract

The article presents a study which aims to determine the DspB/F-binding sites and secretion and translocation signals in the DspA/E protein. The study conducted secretion and translocation assays with the use of serial N-terminal lengths of DspA/E mixed with the active form of AvrRpt2. Results show the presence of secretion and translocation signals in the N terminus of DspA/E, determining that at leas one DspB/F-binding motif is needed for effective translocation into plant cells.

Published
2010
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22. A ubiquitin carboxyl extension protein secreted from a plant-parasitic nematode Globodera rostochiensis is cleaved in planta to promote plant parasitism.

Author

Chronis D, Chen S, Lu S, Hewezi T, Carpenter SC, Loria R, Baum TJ, and Wang X

Subjects
Animals, Helminth Proteins genetics, Molecular Sequence Data, Plants, Genetically Modified genetics, Plants, Genetically Modified metabolism, Plants, Genetically Modified parasitology, Solanum tuberosum genetics, Solanum tuberosum metabolism, Solanum tuberosum parasitology, Nicotiana genetics, Nicotiana metabolism, Nicotiana parasitology, Helminth Proteins metabolism, Tylenchoidea metabolism, Tylenchoidea pathogenicity
Abstract

Nematode effector proteins originating from esophageal gland cells play central roles in suppressing plant defenses and in formation of the plant feeding cells that are required for growth and development of cyst nematodes. A gene (GrUBCEP12) encoding a unique ubiquitin carboxyl extension protein (UBCEP) that consists of a signal peptide for secretion, a mono-ubiquitin domain, and a 12 amino acid carboxyl extension protein (CEP12) domain was cloned from the potato cyst nematode Globodera rostochiensis. This GrUBCEP12 gene was expressed exclusively within the nematode's dorsal esophageal gland cell, and was up-regulated in the parasitic second-stage juvenile, correlating with the time when feeding cell formation is initiated. We showed that specific GrUBCEP12 knockdown via RNA interference reduced nematode parasitic success, and that over-expression of the secreted Gr(Δ) (SP) UBCEP12 protein in potato resulted in increased nematode susceptibility, providing direct evidence that this secreted effector is involved in plant parasitism. Using transient expression assays in Nicotiana benthamiana, we found that Gr(Δ) (SP) UBCEP12 is processed into free ubiquitin and a CEP12 peptide (GrCEP12) in planta, and that GrCEP12 suppresses resistance gene-mediated cell death. A target search showed that expression of RPN2a, a gene encoding a subunit of the 26S proteasome, was dramatically suppressed in Gr(Δ) (SP) UBCEP12 but not GrCEP12 over-expression plants when compared with control plants. Together, these results suggest that, when delivered into host plant cells, Gr(Δ) (SP) UBCEP12 becomes two functional units, one acting to suppress plant immunity and the other potentially affecting the host 26S proteasome, to promote feeding cell formation., (© 2013 The Authors The Plant Journal © 2013 Blackwell Publishing Ltd.)

Published
2013
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