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Background The main cytogenetic studies of the Characidae family comprise the genera Astyanax and Psalidodon involving the use of repetitive DNA probes. However, for the microsatellite classes, studies are still scarce and the function of these sequences in the genome of these individuals is still not understood. Thus, we aimed to analyze and compare the distribution of microsatellite sequences in the species Astyanax bimaculatus and Psalidodon scabripinnis. Methods We collected biopsies from the fins of A. bimaculatus and P. scabripinnis to perform cell culture, followed by chromosome extraction, and mapped the distribution of 14 microsatellites by FISH in both species. Results and Discussion The diploid number observed for both species was 2n = 50, with an acrocentric B microchromosome in A. bimaculatus and a metacentric B chromosome in P. scabripinnis. Regarding FISH, 11 probes hybridized in the karyotype of A. bimaculatus mainly in centromeric regions, and 13 probes hybridized in P. scabripinnis, mainly in telomeric regions, in addition to a large accumulation of microsatellite hybridization on its B chromosome. Conclusion Comparative FISH mapping of 14 microsatellite motifs revealed different patterns of distribution both in autosomes and supernumerary chromosomes of A. bimaculatus and P. scabripinnis, suggesting independent evolutionary processes in each of these species, representing excellent data on chromosome rearrangements and cytotaxonomy.

A pandemia da Covid-19 provocou questionamentos e intensa busca por adaptação às aulas remotas emergenciais. A busca por metodologias para tornar o Ensino de Ciências mais dinâmico e próximo da realidade dos alunos inquietou muitos professores. Nesse sentido, o estudo apresenta uma sequência didática (SD) elaborada e aplicada durante o período letivo de 2020, em três turmas do Ensino Fundamental. A sequência abordou o tema sexualidade com intuito de trazer à sala virtual um clima envolvente, descontraído, de partilha e construção de conhecimento. E assim, nasceu a SQ “Adolescência e Puberdade”, composta por: (1) Sondagem inicial, (2) Abordagem de conteúdo: mudanças corporais, (3) Abordagem de conteúdo: hormônios sexuais, (4) Abordagem de conteúdo: comportamento do adolescente e por fim, (5) Sondagem final. Os resultados fornecem apoio ao docente tornando suas atividades e interações mais participativas, sendo também viável para o trabalho com temas sensíveis, polêmicos e ainda repletos de tabus.

Abstract INTRODUCTION: Female sex workers (FSWs) are considered a bridge for transmission of pathogens from high-risk to general populations. We assessed the epidemiological status of syphilis in FSWs along the Pará highway system. METHODS: Two hundred and twenty-two FSWs were interviewed and samples were analyzed using rapid qualitative tests and real-time polymerase chain reaction. RESULTS: The prevalence of syphilis was high (36.94%). The high rate of prostitution, use of illicit drugs, and search for financial resources increased Treponema pallidum transmission through unprotected sex. CONCLUSIONS: Several characteristics of FSWs were identified, which reinforce the need for measures guaranteeing their health and protection.

O presente estudo buscou diagnosticar o conhecimento de estudantes sobre os aspectos que envolvem a alimentação saudável e a partir deste realizar uma atividade de intervenção de cunho teórico-prático. Dessa forma, foi aplicado um questionário a estudantes do 5º ano do ensino fundamental de uma escola da periferia do município de Capanema, Pará, norte do Brasil. O questionário continha cinco questões fechadas compostas por três alternativas que buscavam observar se os alunos faziam relação ao conteúdo da pirâmide alimentar com a sua dieta alimentar diária. A partir deste estudo pode-se perceber que existe uma grande dificuldade por parte dos estudantes em aplicar os conhecimentos que se trabalham na escola. Para o aluno o mundo da teoria é ainda muito distante da vivência. Auxiliar na consolidação desses conhecimentos trazendo para a vivência desses estudantes é de fundamental importância para estimular o pensar e o agir fazendo com que estes reflitam sobre seus próprios hábitos e possam ser agentes de sua própria saúde.

Introduction: Illicit drug users (DUs) are vulnerable to hepatitis C virus (HCV) infection. The shared use of illicit drugs is the main method of HCV transmission. Methods: A cross-sectional study was conducted in Breves, in northern Brazil. We surveyed 187 DUs to determine the prevalence of and factors associated with HCV infection. Results: The prevalence of anti-HCV antibodies was 36.9%, and the prevalence of hepatitis C virus-ribonucleic acid (HCV-RNA) was 31%. Hepatitis C virus infection was associated with tattoos, intravenous drug use, shared use of equipment for drug use, drug use for longer than 3 years, and daily drug use. Conclusions: Strategies for preventing and controlling HCV transmission should be implemented among DUs.

This study determined the prevalence and factors associated with illicit drug useamongstudents inhighschools in the municipality of Bragança, Pará, northern Brazil. School-based cross-sectional study, a total of five public schools and 1632 students in the municipality ofBragança. The self-administered questionnaire was adapted from other epidemiological studies tothe appropriate population group, with rigorous procedure, guaranteeing anonymity andconfidentiality. The prevalence of illicit drug use among students was 4.37%. Several risk factors tothe use of licit and illicit drugs were detected, of which include: the lack of parental involvement inschool life of students, family income above 3 minimum wages, parents, friends and family whouse illicit drugs and lack of incentive to not use illegal drugs. In summary, this study found thatregular use of illicit drugs is associated with social and economic factors, which should beconsidered in the development of measures to control and prevent the use of illicit drugs in the cityof Bragança.