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1. Loop-mediated isothermal amplification (LAMP) assay coupled with gold nanoparticles for colorimetric detection of Trichoderma spp. in Agaricus bisporus cultivation substrates

Author: Mila Djisalov, Ljiljana Janjušević, Vincent Léguillier, Ljiljana Šašić Zorić, Carole Farre, Jamila Anba-Mondoloni, Jasmina Vidic, and Ivana Gadjanski
Subjects: Medicine, Science
Abstract: Abstract One of the significant challenges in organic cultivation of edible mushrooms is the control of invasive Trichoderma species that can hinder the mushroom production and lead to economic losses. Here, we present a novel loop-mediated isothermal amplification (LAMP) assay coupled with gold nanoparticles (AuNPs) for rapid colorimetric detection of Trichoderma spp. The specificity of LAMP primers designed on the tef1 gene was validated in silico and through gel-electrophoresis on Trichoderma harzianum and non-target soil-borne fungal and bacterial strains. LAMP amplification of genomic DNA templates was performed at 65 °C for only 30 min. The results were rapidly visualized in a microplate format within less than 5 min. The assay is based on salt-induced aggregation of AuNPs that is being prevented by the amplicons produced in case of positive LAMP reaction. As the solution color changes from red to violet upon nanoparticle aggregation can be observed with the naked eye, the developed LAMP-AuNPs assay can be easily operated to provide a simple initial screening for the rapid detection of Trichoderma in button mushroom cultivation substrate.
Published: 2024
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2. Aflatoxin B1 Detection Using a Highly-Sensitive Molecularly-Imprinted Electrochemical Sensor Based on an Electropolymerized Metal Organic Framework

Author: Mengjuan Jiang, Mohamed Braiek, Anca Florea, Amani Chrouda, Carole Farre, Anne Bonhomme, Francois Bessueille, Francis Vocanson, Aidong Zhang, and Nicole Jaffrezic-Renault
Subjects: electrochemical sensors, aflatoxin B1, molecularly imprinted polymers, metal organic framework, gold nanoparticles, Medicine
Abstract: A sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p-aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognize and bind AFB1 through π-π interactions between AFB1 molecules and aniline moities. The performance of the developed sensor for the detection of AFB1 was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as a redox probe, the electron transfer rate increasing when the concentration of AFB1 increases, due to a p-doping effect. The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM. Compared to the non-imprinted sensor, the imprinting factor was found to be 10. Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.
Published: 2015
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3. Responsive Polydiacetylene Vesicles for Biosensing Microorganisms

Author: Estelle Lebègue, Carole Farre, Catherine Jose, Joelle Saulnier, Florence Lagarde, Yves Chevalier, Carole Chaix, and Nicole Jaffrezic-Renault
Subjects: vesicles, polydiacetylene, biosensing, bacteria, toxins, virus, peptides, Chemical technology, TP1-1185
Abstract: Polydiacetylene (PDA) inserted in films or in vesicles has received increasing attention due to its property to undergo a blue-to-red colorimetric transition along with a change from non-fluorescent to fluorescent upon application of various stimuli. In this review paper, the principle for the detection of various microorganisms (bacteria, directly detected or detected through the emitted toxins or through their DNA, and viruses) and of antibacterial and antiviral peptides based on these responsive PDA vesicles are detailed. The analytical performances obtained, when vesicles are in suspension or immobilized, are given and compared to those of the responsive vesicles mainly based on the vesicle encapsulation method. Many future challenges are then discussed.
Published: 2018
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4. A novel adenosine triphosphate (ATP) biosensor based on electrospun polymer nanofibers with entrapped hexokinase and glucose oxidase

Author: Ivan S. Kucherenko, Carole Farre, Gaetan Raimondi, Carole Chaix, Nicole Jaffrezic-Renault, Jean-François Chateaux, Maksim Sobolevskyi, Oleksandr O. Soldatkin, Sergei V. Dzyadevych, Alexei P. Soldatkin, and Florence Lagarde
Subjects: Materials Science (miscellaneous), Cell Biology, Electrical and Electronic Engineering, Physical and Theoretical Chemistry, Atomic and Molecular Physics, and Optics, Biotechnology
Published: 2023
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5. Mechanisms of Influenza Virus HA2 Peptide Interaction with Liposomes Studied by Dual-Wavelength MP-SPR

Author: Meryem Belkilani, Carole Farre, Yves Chevalier, Sylvain Minot, François Bessueille, Adnane Abdelghani, Nicole Jaffrezic-Renault, and Carole Chaix
Subjects: General Materials Science
Abstract: A phospholipid-based liposome layer was used as an effective biomimetic membrane model to study the binding of the pH-dependent fusogenic peptide (E4-GGYC) from the influenza virus hemagglutinin HA2 subunit. To this end, a multiparameter surface plasmon resonance approach (MP-SPR) was used for monitoring peptide-liposome interactions at two pH values (4.5 and 8) by means of recording sensorgrams in real time without the need for labeling. Biotinylated liposomes were first immobilized as a monolayer onto the surface of an SPR gold chip coated with a streptavidin layer. Multiple sets of sensorgrams with different HA2 peptide concentrations were generated at both pHs. Dual-wavelength Fresnel layer modeling was applied to calculate the thickness (
Published: 2022
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6. Click and Bioorthogonal Chemistry: The Future of Active Targeting of Nanoparticles for Nanomedicines?

Author: Carole Chaix, Emmanuel Moreau, Carole Farre, Ludivine Taiariol, Imagerie Moléculaire et Stratégies Théranostiques (IMoST), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA), Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Chemistry, [SDV]Life Sciences [q-bio], Two step, Cancer therapy, Nanoparticle, Nanotechnology, 02 engineering and technology, General Chemistry, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, Nanomedicine, Click chemistry, Nanoparticles, [CHIM]Chemical Sciences, Click Chemistry, Bioorthogonal chemistry, 0210 nano-technology
Abstract: International audience; Over the years, click and bioorthogonal reactions have been the subject of considerable research efforts. These high-performance chemical reactions have been developed to meet requirements not often provided by the chemical reactions commonly used today in the biological environment, such as selectivity, rapid reaction rate and biocompatibility. Click and bioorthogonal reactions have been attracting increasing attention in the biomedical field for the engineering of nanomedicines. In this review, we study a compilation of articles from 2014 to the present, using the terms "click chemistry and nanoparticles (NPs)" to highlight the application of this type of chemistry for applications involving NPs intended for biomedical applications. This study identifies the main strategies offered by click and bioorthogonal chemistry, with respect to passive and active targeting, for NP functionalization with specific and multiple properties for imaging and cancer therapy. In the final part, a novel and promising approach for "two step" targeting of NPs, called pretargeting (PT), is also discussed; the principle of this strategy as well as all the studies listed from 2014 to the present are presented in more detail.
Published: 2021
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7. Specific and sensitive detection of Influenza A virus using a biotin-coated nanoparticle enhanced immunomagnetic assay

Author: Alice Wright, Jasmina Vidic, Carole Chaix, Sara Viezzi, Carole Farre, Nathalie Lejal, Marisa Manzano, Perrine Robin, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Dipartimento di Scienze AgroAlimentari, Ambientali e Animali - [Udine] (DI4A), Università degli Studi di Udine - University of Udine [Italie], Virologie et Immunologie Moléculaires (VIM (UR 0892)), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), and AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: viruses, Biotin, Context (language use), 02 engineering and technology, medicine.disease_cause, Sensitivity and Specificity, 01 natural sciences, Biochemistry, Analytical Chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Lysis buffer, medicine, Influenza A virus, [CHIM]Chemical Sciences, Magnetic bead, Diagnostics, Paper in Forefront, Nucleoprotein, Immunoassay, Detection limit, Chromatography, medicine.diagnostic_test, Immunomagnetic Separation, Biotin-nanoparticle, Chemistry, 010401 analytical chemistry, Antibodies, Monoclonal, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Biotinylation, Nanoparticles, Respiratory virus, 0210 nano-technology
Abstract: This study reports the development of a sensitive magnetic bead-based enzyme-linked immunoassay (MELISA) for the pan-reactive detection of the Influenza A virus. The assay combines immunomagnetic beads and biotin-nanoparticle-based detection to quantify a highly conserved viral nucleoprotein in virus lysates. At the capture step, monoclonal antibody-coated magnetic microbeads were used to bind and concentrate the nucleoprotein in samples. The colorimetric detection signal was amplified using biotinylated silica nanoparticles (NP). These nanoparticles were functionalized on the surface with short DNA spacers bearing biotin groups by an automated supported synthesis method performed on nano-on-micro assemblies with a DNA/RNA synthesizer. A biotin-nanoparticle and immunomagnetic bead-based assay was developed. We succeeded in detecting Influenza A viruses directly in the lysis buffer supplemented with 10% saliva to simulate the clinical context. The biotin-nanoparticle amplification step enabled detection limits as low as 3 × 103 PFU mL−1 and 4 × 104 PFU mL−1 to be achieved for the H1N1 and H3N2 strains respectively. In contrast, a classical ELISA test based on the same antibody sandwich showed detection limit of 1.2 × 107 PFU mL−1 for H1N1. The new enhanced MELISA proved to be specific, as no cross-reactivity was found with a porcine respiratory virus (PRRSV). Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00216-020-03081-x.
Published: 2020
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8. Surface‐enhanced Raman spectroscopy using uncoated gold nanoparticles for bacteria discrimination

Author: Carine Commun, Claire Bordes, Sandrine Bourgeois, François Bessueille, Carole Farre, Elie Akanny, Anne Bonhomme, Anne Doléans-Jordheim, Chemometrics and Theoretical Chemistry - Chimiométrie et chimie théorique, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'automatique, de génie des procédés et de génie pharmaceutique (LAGEPP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Surfaces, Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de Bactériologie de l'Hôpital de la Croix-Rousse, Hospices Civils de Lyon (HCL), Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Pharmaceutiques et Biologiques (ISPB), and Université de Lyon-Université de Lyon
Subjects: 02 engineering and technology, medicine.disease_cause, 01 natural sciences, Silver nanoparticle, symbols.namesake, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, medicine, Molecule, General Materials Science, Escherichia coli, Spectroscopy, biology, Chemistry, 010401 analytical chemistry, Substrate (chemistry), Surface-enhanced Raman spectroscopy, 021001 nanoscience & nanotechnology, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, 0104 chemical sciences, Colloidal gold, symbols, 0210 nano-technology, Raman spectroscopy, [CHIM.CHEM]Chemical Sciences/Cheminformatics, Bacteria, Nuclear chemistry
Abstract: International audience; In this work, surface-enhanced Raman spectroscopy (SERS) method based on uncoated spherical gold nanoparticles as SERS substrate was applied for the detection and discrimination of three different bacterial strains: Gram-positive Bacillus subtilis and Lactobacillus rhamnosus GG and Gram-negative Escherichia coli. The exploitation of bacteria SERS spectra by multivariate data analysis (principal component analysis and partial least squares-discriminant analysis) proved the robustness of the developed method and its suitability to discriminate between the three different strains despite their relatively similar Raman signals. The SERS performance of the developed method was compared with that of the classical silver nanoparticles indicating a better bacteria signal enhancement, up to 15 times higher in case of E. coli, likely due to stronger interactions of gold nanoparticles with components such as purine molecules that show dominant bands in the bacterial SERS spectra. K E Y W O R D S bacteria detection, bacteria discrimination, gold nanoparticles, SERS
Published: 2020
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9. Self‐Organized 3D Graphene as a Robust Sensing Platform

Author: Nicole Jaffrezic-Renault, V. Barnier, Gaetan Raimondi, Florence Garrelie, Florent Bourquard, Florence Lagarde, Carole Chaix, Anne-Sophie Loir, Francis Vocanson, Carole Farre, and C. Donnet
Subjects: Materials science, Graphene, law, Nanotechnology, Surface-enhanced Raman spectroscopy, law.invention
Published: 2019
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10. Surface Plasmon Resonance Monitoring of Mono-Rhamnolipid Interaction with Phospholipid-Based Liposomes

Author: Carole Chaix, François Bessueille, Maryam Shokouhi, Carole Farre, Sylvain Minot, Yves Chevalier, Meryem Belkilani, A. Abdelghani, Nicole Jaffrezic-Renault, Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), ENSIT - University of Tunis, Research Unit of Nanobiotechnology and Valorisation of Medicinal Plants, INSAT - University of Carthage, Department of chemistry, University of Isfahan, Laboratoire d'automatique, de génie des procédés et de génie pharmaceutique (LAGEPP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Surfaces, and Micro & Nanobiotechnologies
Subjects: Streptavidin, education, Phospholipid, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Rhamnose, chemistry.chemical_compound, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Monolayer, Electrochemistry, [CHIM]Chemical Sciences, General Materials Science, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Vesicles, Surface plasmon resonance, Lipid bilayer, Surface plasmon resonance spectroscopy, Spectroscopy, ComputingMilieux_MISCELLANEOUS, Phospholipids, Liposome, Vesicle, Decanoates, Surfaces and Interfaces, Surface Plasmon Resonance, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 0104 chemical sciences, Membrane, chemistry, Liposomes, Biophysics, Gold, 0210 nano-technology, Layers
Abstract: The interactions of mono-rhamnolipids (mono-RLs) with model membranes were investigated through a biomimetic approach using phospholipid-based liposomes immobilized on a gold substrate and also by the multiparametric surface plasmon resonance (MP-SPR) technique. Biotinylated liposomes were bound onto an SPR gold chip surface coated with a streptavidin layer. The resulting MP-SPR signal proved the efficient binding of the liposomes. The thickness of the liposome layer calculated by modeling the MP-SPR signal was about 80 nm, which matched the average diameter of the liposomes. The mono-RL binding to the film of the phospholipid liposomes was monitored by SPR and the morphological changes of the liposome layer were assessed by modeling the SPR signal. We demonstrated the capacity of the MP-SPR technique to characterize the different steps of the liposome architecture evolution, i.e., from a monolayer of phospholipid liposomes to a single phospholipid bilayer induced by the interaction with mono-RLs. Further washing treatment with Triton X-100 detergent left a monolayer of phospholipid on the surface. As a possible practical application, our method based on a biomimetic membrane coupled to an SPR measurement proved to be a robust and sensitive analytical tool for the detection of mono-RLs with a limit of detection of 2 μg mL-1.
Published: 2021
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11. Boron doped graphene synthesis using pulsed laser deposition and its electrochemical characterization

Author: Carole Chaix, Florence Garrelie, Christophe Donnet, Carole Farre, Florent Bourquard, Jules Galipaud, Anne-Sophie Loir, Y. Bleu, Vincent Barnier, Laboratoire Hubert Curien [Saint Etienne] (LHC), Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS), Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), École Centrale de Lyon (ECL), Université de Lyon, École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), and Institut Mines-Télécom [Paris] (IMT)
Subjects: Materials science, Heteroatom, chemistry.chemical_element, 02 engineering and technology, 010402 general chemistry, Electrochemistry, 01 natural sciences, Pulsed laser deposition, law.invention, [SPI.MAT]Engineering Sciences [physics]/Materials, symbols.namesake, X-ray photoelectron spectroscopy, law, Materials Chemistry, [CHIM]Chemical Sciences, Electrical and Electronic Engineering, Boron, ComputingMilieux_MISCELLANEOUS, Graphene, Mechanical Engineering, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Electronic, Optical and Magnetic Materials, Chemical engineering, chemistry, Electrode, symbols, 0210 nano-technology, Raman spectroscopy
Abstract: During the design of graphene (G), their electronic, chemical and electrochemical properties among others can be tuned by incorporating heteroatoms in the honeycomb structure. In this study, we investigated boron doped graphene (BG) synthesis and the effect of boron content on the electrochemical property of graphene. To this end, BG was synthesized using pulsed laser deposition (PLD) with carbon and boron as solid sources, followed by rapid thermal annealing in the presence of nickel catalyst. Raman spectroscopy revealed that the BG films consisted of few-layer graphene and boron doping increased the defects level in the films. X-ray photoelectron spectroscopy confirmed the successful boron doping into the graphene with the control of boron content. Electrochemical characterization demonstrated that BG electrodes exhibit higher reversibility and electron transfer constant than the G electrode. All these results prove that PLD method is a viable alternative way to produce BG films with excellent electrochemical properties.
Published: 2021
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12. Highly sensitive detection of Campylobacter spp. In chicken meat using a silica nanoparticle enhanced dot blot DNA biosensor

Author: Carole Chaix, Marisa Manzano, Jasmina Vidic, Priya Vizzini, Thierry Meylheuc, Nalini Ramarao, Carole Farre, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), The authors thank the Centre Technologique des Microstructures of the Lyon 1 University for providing access to its TEM facilities, and the MIMA2 platform Jouy en Josas for access to electron microscopy equipment (MIMA2, INRAE, 2018. Microscopy and Imaging Facility for Microbes, Animals and Foods, https://doi.org/10.15454/1.5572348210007727E12).JV thanks Maria-Vesna Nikolic (University of Belgrade, Serbia) for English editing. PV acknowledges a doctoral fellowship from the University of Udine, Italy. This research was supported in part by the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 872662 (IPANEMA), the European Union's Horizon 2020 research and by the University Paris-Saclay through the Poc in labs 2019 grant agreement No 00003469 (OSCAR)., European Project: 872662 ,IPANEMA, Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)
Subjects: Campylobacter, DNA dot Blot, Food safety, Multiplex bacterial detection, Si-nanoparticles, Meat, DNA polymerase, Biomedical Engineering, Biophysics, Dot blot, Food Contamination, Biosensing Techniques, 02 engineering and technology, 01 natural sciences, Horseradish peroxidase, Luminol, chemistry.chemical_compound, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Electrochemistry, Animals, [CHIM]Chemical Sciences, Multiplex, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Chromatography, biology, Chemistry, 010401 analytical chemistry, technology, industry, and agriculture, DNA, Hydrogen Peroxide, General Medicine, Silicon Dioxide, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Blot, Biotinylation, biology.protein, Nanoparticles, Campylobacter, DNA dot Blot, Si-nanoparticles, Food safety, Multiplex bacterial detection, 0210 nano-technology, Chickens, Biosensor, Biotechnology
Abstract: The authors thank the Centre Technologique des Microstructures of the Lyon 1 University for providing access to its TEM facilities, and the MIMA2 platform Jouy en Josas for access to electron microscopy equipment (MIMA2, INRAE, 2018. Microscopy and Imaging Facility for Microbes, Animals and Foods, https://doi.org/10.15454/1. 5572348210007727E12). JV thanks Maria-Vesna Nikolic (University of Belgrade, Serbia) for English editing.; International audience; Paper-based DNA biosensors are powerful tools in point-of-care diagnostics since they are affordable, portable, user-friendly, rapid and robust. However, their sensitivity is not always as high as required to enable DNA quantification. To improve the response of standard dot blots, we have applied a new enhancement strategy that increases the sensitivity of assays based on the use of biotinylated silica-nanoparticles (biotin-Si-NPs). After immobilization of a genomic Campylobacter DNA onto a paper membrane, and addition of a biotinylated-DNA detection probe, hybridization was evidenced using streptavidin-conjugated to horseradish peroxidase (HRP) in the presence of luminol and H2O2. Replacement of the single biotin by the biotin-Si-NPs boosted on average a 30 fold chemiluminescent read-out of the biosensor. Characterization of biotin-Si-NPs onto a paper with immobilized DNA was done using a scanning electron microscope. A limit of detection of 3 pg/μL of DNA, similar to the available qPCR kits, is achieved, but it is cheaper, easier and avoids inhibition of DNA polymerase by molecules from the food matrices. We demonstrated that the new dot blot coupled to biotin-Si-NPs successfully detected Campylobacter from naturally contaminated chicken meat, without needing a PCR step. Hence, such an enhanced dot blot paves the path to the development of a portable and multiplex paper based platform for point-of-care screening of chicken carcasses for Campylobacter.
Published: 2021
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13. Highly Sensitive Detection of Campylobacter spp. in Chicken Meat using a Silica Nanoparticle Enhanced Dot Blot DNA Biosensor

Author: Marisa Manzano, Carole Chaix, Nalini Ramarao, Carole Farre, Jasmina Vidic, Priya Vizzini, and Thierry Meylheuc
Subjects: Blot, chemistry.chemical_compound, Chromatography, biology, DNA polymerase, Chemistry, Biotinylation, biology.protein, Dot blot, Multiplex, Horseradish peroxidase, Biosensor, Luminol
Abstract: Paper-based DNA biosensors are powerful tools in point-of-care diagnostics since they are affordable, portable, user-friendly, rapid and robust. However, their sensitivity is not always as high as required to enable DNA quantification. To improve the response of standard dot blots, we have applied a new enhancement strategy that increases the sensitivity of assays based on the use of biotinylated silica-nanoparticles (biotin-Si-NPs). After immobilization of a genomic Campylobacter DNA onto a paper membrane, and addition of a biotinylated-DNA detection probe, hybridization was evidenced using streptavidin-conjugated to horseradish peroxidase (HRP) in the presence of luminol and H2O2. Replacement of the single biotin by the biotin-Si-NPs boosted on average a 30 fold chemiluminescent read-out of the biosensor. Characterization of biotin-Si-NPs onto a paper with immobilized DNA was done using a scanning electron microscope. A limit of detection of 3 pg/μL of DNA, similar to the available qPCR kits, is achieved, but it is cheaper, easier and avoids inhibition of DNA polymerase by molecules from the food matrices. We demonstrated that the new dot blot coupled to biotin-Si-NPs successfully detected Campylobacter from naturally contaminated chicken meat, without needing a PCR step. Hence, such an enhanced dot blot paves the path to the development of a portable and multiplex paper based platform for point-of-care screening of chicken carcasses for Campylobacter.Graphical abstract
Published: 2020
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14. Highly labeled methylene blue-ds DNA silica nanoparticles for signal enhancement of immunoassays: application to the sensitive detection of bacteria in human platelet concentrates

Author: Jeannette Fareh, Lionel Valera, Carole Farre, Carole Chaix, Ludivine Vossier, Agnès Pouzet, Nicole Jaffrezic-Renault, Typhaine Dagland, Fanny Leon, Romaric Bonnet, Chantal Fournier-Wirth, Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Sysdiag-Modélisation et Ingénierie des Systèmes Complexes Biologiques pour le Diagnostic (SysDiag ), BIO-RAD-Centre National de la Recherche Scientifique (CNRS), Laboratoire TransDiag, Etablissement Français du Sang, Pathogénèse et contrôle des infections chroniques (PCCI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), Laboratoire de recherche, Etablissement Français du Sang Pyrénées-Méditerranée, and This work was supported by Bio-Rad Laboratories.
Subjects: Blood Platelets, Nanoparticle, Biosensing Techniques, 02 engineering and technology, 01 natural sciences, Biochemistry, Analytical Chemistry, Matrix (chemical analysis), chemistry.chemical_compound, Limit of Detection, Escherichia coli, Electrochemistry, medicine, Humans, [CHIM]Chemical Sciences, Environmental Chemistry, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Methylene, Spectroscopy, Immunoassay, Detection limit, Chromatography, medicine.diagnostic_test, Oligonucleotide, 010401 analytical chemistry, DNA, Electrochemical Techniques, Silicon Dioxide, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Methylene Blue, chemistry, Nanoparticles, 0210 nano-technology, Methylene blue
Abstract: The authors would like to express their sincere gratitude to Gaelle-Anne Cremer for furnishing the streptavidin-antibody conjugates, Patrice Sarfati and Pierre Sonigo for their expert supervision of the work, François Rieunier and Stephane Rihet for their pertinent advices on the work.; International audience; A nanoparticle-based electrochemical sandwich immunoassay was developed for bacteria detection in platelet concentrates. For the assay, magnetic beads were functionalized with antibodies to allow the specific capture of bacteria from the complex matrix, and innovative methylene blue-DNA/nanoparticle assemblies provided the electrochemical response for amplified detection. This nanoparticular system was designed as a temperature-sensitive nano-tool for electrochemical detection. First, oligonucleotide-functionalized nanoparticles were obtained by direct synthesis of the DNA strands on the nanoparticle surface using an automated oligonucleotide synthesizer. Densely packed DNA coverage was thus obtained. Then, DNA duplexes were constructed on the NP surface with a complementary strand bearing a 3 methylene blue tag. This strategy ultimately produced highly functionalized nanoparticles with electrochemical markers. These assemblies enabled amplification of the electrochemical signal, resulting in a very good sensitivity. A proof-of-concept was carried out for E. coli detection in human platelet concentrates. Bacterial contamination of this complex biological matrix is the highest residual infectious risk in blood transfusion. The development of a rapid assay that could reach 10–102 CFU mL−1 sensitivity is a great challenge. The nanoparticle-based electrochemical sandwich immunoassay carried out on a boron doped diamond electrode proved to be sensitive for E. coli detection in human platelets. Two antibody pairs were used to develop either a generic assay against certain Gram negative strains or a specific assay for E. coli. The methylene blue-DNA/nanoparticles amplify sensitivity ×1000 compared with the assay run without NPs for electrochemical detection. A limit of detection of 10 CFU mL−1 in a biological matrix was achieved for E. coli using the highly specific antibody pair.
Published: 2018
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15. Self-organized 3D graphene as a robust sensing platform

Author: Bourquand, F., Donnet, C., Florence GARRELIE, S Loir, A., Francis Vocanson, Carole Chaix, Carole Farre, Nicole Jaffrezic-Renault, Florence Lagarde, Gaëtan Raimondi, Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), in Palys Barbara (Ed), and Bussy, Agnès
Subjects: [CHIM] Chemical Sciences, [CHIM]Chemical Sciences, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2019

16. Combining culture and microbead-based immunoassay for the early and generic detection of bacteria in platelet concentrates

Author: Jeannette Fareh, Typhaine Dagland, Ludivine Vossier, Stéphanie Roche, Agnès Pouzet, Fanny Leon, Carole Farre, Christine Favier, Laetitia Rubrecht, Chantal Fournier-Wirth, Stéphane Rihet, Carole Chaix, Romaric Bonnet, Nicole Jaffrezic-Renault, Lionel Valera, Pascale Galea, Gaelle‐Anne Cremer, Dominique Piquer, Laboratoire TransDiag, Etablissement Français du Sang, Pathogénèse et contrôle des infections chroniques (PCCI), Centre Hospitalier Universitaire de Montpellier (CHU Montpellier )-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Sysdag, Sysdiag CNRS Bio-Rad UMR 3145, Cap Delta/Parc Euromédecine, Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), BIO-RAD, Sysdiag-Modélisation et Ingénierie des Systèmes Complexes Biologiques pour le Diagnostic (SysDiag ), BIO-RAD-Centre National de la Recherche Scientifique (CNRS), Systèmes d'élevage, nutrition animale et humaine (SENAH), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Centre National de la Recherche Scientifique (CNRS)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École normale supérieure - Lyon (ENS Lyon), Département de Chimie Moléculaire (DCM), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier )-Université de Montpellier (UM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: Acinetobacter baumannii, Blood Platelets, Microbiological culture, Immunology, 030204 cardiovascular system & hematology, Bacterial growth, Microbiology, 03 medical and health sciences, 0302 clinical medicine, medicine, Escherichia coli, Immunology and Allergy, Humans, Platelet, Serratia marcescens, ComputingMilieux_MISCELLANEOUS, Immunoassay, biology, medicine.diagnostic_test, Bacteria, Chemistry, Klebsiella oxytoca, Antibodies, Monoclonal, Hematology, Microbead (research), biology.organism_classification, Antibody production, Pseudomonas aeruginosa, biology.protein, Antibody, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, 030215 immunology
Abstract: BACKGROUND Despite current preventive strategies, bacterial contamination of platelets is the highest residual infectious risk in transfusion. Bacteria can grow from an initial concentration of 0.03-0.3 colony-forming units (CFUs)/mL up to 108 to 109 CFUs/mL over the product shelf life. The aim of this study was to develop a cost-effective approach for an early, rapid, sensitive, and generic detection of bacteria in platelet concentrates. STUDY DESIGN AND METHODS A large panel of bacteria involved in transfusion reactions, including clinical isolates and reference strains, was established. Sampling was performed 24 hours after platelet spiking. After an optimized culture step for increasing bacterial growth, a microbead-based immunoassay allowed the generic detection of bacteria. Antibody production and immunoassay development took place exclusively with bacteria spiked in fresh platelet concentrates to improve the specificity of the test. RESULTS Antibodies for the generic detection of either gram-negative or gram-positive bacteria were selected for the microbead-based immunoassay. Our approach, combining the improved culture step with the immunoassay, allowed sensitive detection of 1 to 10 CFUs/mL for gram-negative and 1 to 102 CFUs/mL for gram-positive species. CONCLUSION In this study, a new approach combining bacterial culture with immunoassay was developed for the generic and sensitive detection of bacteria in platelet concentrates. This efficient and easily automatable approach allows tested platelets to be used on Day 2 after collection and could represent an alternative strategy for reducing the risk of transfusion-transmitted bacterial infections. This strategy could be adapted for the detection of bacteria in other cellular products.
Published: 2019
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17. Biomimetic vesicles for electrochemical sensing

Author: Carole Chaix, Carole Farre, Yves Chevalier, Catherine Jose, Estelle Lebègue, Florence Lagarde, Nicole Jaffrezic-Renault, Joëlle Saulnier, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes-Centre National de la Recherche Scientifique (CNRS), Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Laboratoire d'automatique et de génie des procédés (LAGEP), Université de Lyon-Université de Lyon-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Sciences Analytiques (SA), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Laboratoire d'automatique, de génie des procédés et de génie pharmaceutique (LAGEPP), EC, Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Materials science, Vesicle, 010401 analytical chemistry, Nanotechnology, 02 engineering and technology, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, Key issues, 01 natural sciences, 0104 chemical sciences, Analytical Chemistry, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, Membrane, [CHIM.POLY]Chemical Sciences/Polymers, [SDV.SP.PG]Life Sciences [q-bio]/Pharmaceutical sciences/Galenic pharmacology, Microfluidic chip, Electrochemistry, Electrochemical biosensor, 0210 nano-technology, Biosensor, ComputingMilieux_MISCELLANEOUS
Abstract: International audience; Biomimetic vesicles, mainly composed of self-assembled bilayers of phospholipids, have attracted great attention for applications in the biosensor field over a number of decades, as a means to amplify the signal through encapsulated signal probes. In this review paper the most important developments in biomimetic vesicles for electrochemical biosensing within the last 2 years are presented, with a focus on the format of bioassays, their inclusion in microfluidic chip devices and their use in mimicking cell membranes. Key issues and the remaining challenges for future commercialization are analyzed.
Published: 2018
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18. Responsive Polydiacetylene Vesicles for Biosensing Microorganisms

Author: Joëlle Saulnier, Carole Chaix, Florence Lagarde, Carole Farre, Catherine Jose, Yves Chevalier, Nicole Jaffrezic-Renault, and Estelle Lebègue
Subjects: chemistry.chemical_compound, biology, Chemistry, Microorganism, Vesicle, Biophysics, biology.organism_classification, Biosensor, Fluorescence, DNA, Bacteria
Abstract: Polydiacetylene (PDA) inserted in films or in vesicles have received increasing attention due to PDA property to undergo a blue-to-red colorimetric transition along with a change from non-fluorescent to fluorescent upon application of various stimuli. In this review paper, the principle of the detection of various microorganisms (bacteria: directly detected or detected through the emitted toxins or through their DNA, and viruses) and of antibacterial and antiviral peptides based on these responsive PDA vesicles are detailed. The obtained analytical performances, when vesicles are in suspension or immobilized, are given and compared to those of the responsive vesicles mainly based on the vesicle encapsulation method. Many future challenges are then discussed.
Published: 2018
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19. Label-free electrochemical monitoring of protein addressing through electroactivated 'click' chemistry on gold electrodes

Author: Nicole Jaffrezic-Renault, Gabriel De Crozals, Carole Chaix, Nadir Meini, Carole Farre, R. Kherrat, Micaël Ripert, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratory of Environmental Engineering, Université Badji Mokhtar Annaba (UBMA), ANR (ANR-09-PIRI-0023), AUF, EC (NANODEV project FP7-PEOPLE-2012-IRSES-318524), CAMPUS-France (PHC Maghreb no. 27960UG)., ANR-09-PIRI-0023,VirProbe,Conception d'un microsystème basé sur des sondes électrochimiques ultra-sensibles pour une multi-détection sans marquage d'acides nucléiques: application au génotypage HCV(2009), European Project, Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), and Université Badji Mokhtar - Annaba [Annaba] (UBMA)
Subjects: Streptavidin, Surface Properties, Gold chip, Analytical chemistry, Biotin, Bioengineering, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Biomaterials, chemistry.chemical_compound, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Electrochemistry, Humans, Biotinylation, Electroaddressing, Electrodes, Serum Albumin, Phosphoramidite, "Click" reaction, Staining and Labeling, Dithiol, Click" reaction, Pentetic Acid, 021001 nanoscience & nanotechnology, Combinatorial chemistry, 0104 chemical sciences, Dielectric spectroscopy, Protein immobilization, Microscopy, Fluorescence, chemistry, Mechanics of Materials, Dielectric Spectroscopy, Reagent, Calibration, Click chemistry, Surface modification, Click Chemistry, Gold, Azide, 0210 nano-technology, Electrochemical impedance spectroscopy, Protein Binding
Abstract: International audience; In this work, using electrochemical impedance spectroscopy (EIS), we have, for the first time, label-free monitored protein immobilization on a gold surface through a strategy of electroaddressing, compatible with the production of microarrays for multi-detection. This functionalization is achieved via the alkyne/azide cycloaddition, better known as the "click" reaction. The electroaddressing was applied to a polythiol hexynyl derivative previously grafted onto the gold surface. This compound consists of two dithiol phosphate groups and a hexynyl function and was synthesized through a supported synthesis approach, from a dithiol reagent, phosphoramidite (DTPA), and a hexynyl phosphoramidite. Next, an azide-PEG3-biotin derivative was grafted onto the modified gold surface by electro-chronocoulometry. The "click" reaction was controlled by electrochemical impedance spectroscopy, showing the change in impedance only when the electroaddressing was performed at − 300 mV. No effect on the EIS signal was observed when a positive potential was applied, confirming the specificity of the electroactivation. Biotin-modified electrodes were used to fix streptavidin and the immobilization was monitored using EIS. Fluorescent streptavidin-functionalized silica nanoparticles were also specifically grafted onto the biotinylated gold surface in order to confirm the "click" reaction using fluorescence microscopy. The obtained streptavidin platform was used to detect the surface coverage by biotinylated human serum albumin (HSA). The lowest detectable concentration is 10 pg/mL, and surface saturation is obtained with concentrations higher than 100 ng/mL
Published: 2014
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20. Selective functionalization of Au electrodes by electrochemical activation of the 'click' reaction catalyst

Author: Micaël Ripert, Carole Farre, Carole Chaix, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), and the ANR (ANR-09-PIRI-0023)
Subjects: Gold electrode, General Chemical Engineering, TRANSFER KINETICS, SINGLE-NUCLEOTIDE MISMATCHES, Alkyne, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, chemistry.chemical_compound, SELF-ASSEMBLED MONOLAYERS, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Click reaction, Electrochemistry, Organic chemistry, GOLD, FERROCENE, chemistry.chemical_classification, Bioconjugation, SURFACES, DNA HYBRIDIZATION, MODIFIED OLIGONUCLEOTIDES, Addressing, 021001 nanoscience & nanotechnology, Combinatorial chemistry, Cycloaddition, 0104 chemical sciences, Electrochemical gas sensor, Electrochemical sensor, chemistry, Click chemistry, Surface modification, BIOSENSORS, GENE DETECTION, Cyclic voltammetry, 0210 nano-technology, Electrochemical catalysis, Derivative (chemistry)
Abstract: International audience; The potential-assisted copper-catalyzed alkyne-azide cycloaddition was investigated to modify a gold electrode surface. Firstly, a tetrathiol-hexynyl derivative was used to introduce alkyne functions on the surface. This anchor proved its robustness in conditions used for the "click" reaction and in wet storage. Then, the potential-assisted "click" reaction was studied with an azido ferrocene derivative. The experimental conditions were optimized according to the electrochemical response of the sensor by cyclic voltammetry and the "click" reaction yield was established. After functionalization, the presence of the 1,2,3-triazole group was confirmed by XPS. A fluorescent azido oligonucleotide was grafted onto the gold surface allowing visualization of the reaction by fluorescence microscopy.
Published: 2013
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21. Gold Nanoparticle/Polymer/Enzyme Nanocomposite for the Development of Adenosine Triphosphate Biosensor

Author: A. P. Soldatkin, Florence Lagarde, Carole Chaix, I. S. Kucherenko, Oleksandr O. Soldatkin, Carole Farre, Nicole Jaffrezic-Renault, and Gaetan Raimondi
Subjects: Polyethylenimine, biology, 010401 analytical chemistry, technology, industry, and agriculture, 02 engineering and technology, 021001 nanoscience & nanotechnology, 01 natural sciences, Polyvinyl alcohol, Molecular biology, Amperometry, 0104 chemical sciences, Matrix (chemical analysis), chemistry.chemical_compound, chemistry, Colloidal gold, biology.protein, Glucose oxidase, Bovine serum albumin, 0210 nano-technology, Biosensor, Nuclear chemistry
Abstract: Development of the enzyme-containing nanocomposites provides an excellent opportunity for the development of the sensitive and effective analytical devices – biosensors. In the present work, we used the nanocomposites that contained two enzymes (glucose oxidase and hexokinase), polymers, and gold nanoparticles (GNPs). Such nanostructure was expected to increase electron transfer in the bioselective element of biosensor and to improve the enzyme stability during the immobilization process. An amperometric biosensor based on the bienzyme system has been developed. The biosensor is sensitive to adenosine-5′-triphosphate (ATP) and glucose. The enzymes were immobilized onto the surface of a platinum disk electrode that was used as amperometric transducer. Three different methods of immobilization were investigated: cross-linking of the enzymes in the presence of bovine serum albumin, entrapment in a photo-cross-linkable modified polyvinyl alcohol (PVA-SbQ) matrix, and entrapment of the enzymes in a PVA/polyethylenimine matrix. The best results during the ATP determination were obtained with PVA-SbQ, and this immobilization method was modified by addition of 18-nm GNPs to the reacting mixture. The ATP detection could be achieved in all cases except for PVA/polyethylenimine, the best sensitivity and linear range being achieved by co-immobilizing glucose oxidase/hexokinase into the photo-cross-linked PVA-SbQ/GNP polymer matrix.
Published: 2017
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22. Boron-doped Diamond Electrodes Modified with Fe 3 O 4 @Au Magnetic Nanocomposites as Sensitive Platform for Detection of a Cancer Biomarker, Interleukin-8

Author: Abdelhamid Errachid, Abdoullatif Baraket, Yi Yang, Aidong Zhang, Mohamed Braiek, Carole Farre, Carole Chaix, François Bessueille, Nicole Jaffrezic-Renault, Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Key Lab Pesticide & Chem Biol, Central China Normal University [Wuhan, China], Surfaces, Micro & Nanobiotechnologies, The authors thank the EC through the Marie Curie NANODEV Grant No318524 for financial support. We acknowledge the funding through the European Communities Seventh Framework Programme entitled Sea-on-a-Chip (FP7-OCEAN-2013) (No.614168) and the European Union's Horzion 2020 Research and Innovation Programme entitled HEARTEN under grant agreement No.643694., European Project: 643694,H2020,H2020-PHC-2014-single-stage,HEARTEN(2015), European Project: 614168,EC:FP7:KBBE,FP7-OCEAN-2013,SEA-ON-A-CHIP(2013), European Project: 318524,EC:FP7:PEOPLE,FP7-PEOPLE-2012-IRSES,NANODEV(2013), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Detection limit, Nanocomposite, Analytical chemistry, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, Electrochemistry, 01 natural sciences, 0104 chemical sciences, Analytical Chemistry, Dielectric spectroscopy, chemistry.chemical_compound, chemistry, Colloidal gold, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Magnetic nanoparticles, Ferricyanide, Cyclic voltammetry, 0210 nano-technology, Nuclear chemistry
Abstract: International audience; Interleukin-8 (IL-8), a dimeric protein composed of two identical subunits, is overexpressed in different human cancers, among them pancreatic cancers and head and neck cancers. There is a strong demand for monitoring of this biomarker protein for early cancer detection. A new concept of label-free impedimetric immunosensors for IL-8 detection is described in this work. A nanocomposite of gold nanoparticles (AuNPs) decorating a magnetic Fe3O4 core is synthesized using cysteamine as linker, and characterized using TEM and UV-Vis spectrometry. After immobilization of these Fe3O4@Au magnetic nanocomposites on a boron doped diamond (BDD) electrode using a simple magnet, anti-human IL-8 monoclonal antibody is linked to the AuNPs, previously functionalized with thioctic acid. The functionalization of the immunosensor was characterized using two electrochemical techniques: cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), performed in phosphate buffer with ferro/ferricyanide as the redox probe. The EIS technique was used for affinity assays: antibody-Antigen binding. A linear relationship between the increment in the electron transfer resistance (R-CT) and the logarithmic value of IL-8 concentration was observed between 0.1pg/mL and 1000pg/mL. The limit of detection (LOD) was estimated at 0.03pg/mL.
Published: 2016
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23. Semiconducting properties of thin films with embedded nanoparticles

Author: Loïc J. Blum, Christophe A. Marquette, Carole Farre, Marcus F. Lawrence, Volodymyr Lysenko, Carole Chaix, Mouhssine Benlarbi, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Sciences Analytiques (SA), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), and Depierre, Frédérique
Subjects: Thin-film, Materials science, Fabrication, Silicon, Nanoparticle, Mineralogy, chemistry.chemical_element, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Spin-coating, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Materials Chemistry, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Thin film, Spin coating, Nanocomposite, business.industry, Mechanical Engineering, Metals and Alloys, Impedance, Semiconductor, Epoxy, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 0104 chemical sciences, Electronic, Optical and Magnetic Materials, chemistry, Mechanics of Materials, visual_art, visual_art.visual_art_medium, Nanoparticles, Optoelectronics, 0210 nano-technology, business
Abstract: International audience; We demonstrate here the possibility of designing semiconducting thin films with controlled electrochemical properties. The thin films are composed of (i) an insulating binder and (ii) a semiconductor nanopowder which enables the fine tuning of the semiconducting properties of the layers. Thus, p-and n-type silicon particles (obtained from a top-down technique), or metal-oxide ZnO, SnO(2) and NiO nanoparticles (synthesized using a bottom-up protocol) are successfully integrated into spin-coated or screen-printed thin films and used as semiconducting materials. The flat band potential (Vfb) of the films is then easily tuned from DV to -1.138 V. (C) 2010 Elsevier B.V. All rights reserved.
Published: 2010
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24. Effect of Perfluorinated-Hexaethylene Glycol Functionalization of Gold Nanoparticles on the Enhancement of the Response of an Enzymatic Conductometric Biosensor for Urea Detection

Author: Carole Farre, Nicole Jaffrezic-Renault, Mohamed Braiek, Mengjuan Jiang, Anne Bonhomme, Carole Chaix, Aidong Zhang, Jean-François Chateaux, Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), spectroscopies vibrationnelles, INL - Lab-On-Chip et Instrumentation (INL - LOCI), Institut des Nanotechnologies de Lyon (INL), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Lyon (ECL), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Key Lab Pesticides & Chem Biol, Central China Normal University [Wuhan, China], and Bussy, Agnès
Subjects: [CHIM.ANAL] Chemical Sciences/Analytical chemistry, Materials science, Urease, Analytical chemistry, Nanoparticle, 02 engineering and technology, 010501 environmental sciences, 01 natural sciences, chemistry.chemical_compound, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Spectrophotometry, medicine, 0105 earth and related environmental sciences, Detection limit, biology, medicine.diagnostic_test, General Medicine, 021001 nanoscience & nanotechnology, chemistry, Colloidal gold, biology.protein, Urea, Surface modification, 0210 nano-technology, Biosensor, Nuclear chemistry
Abstract: International audience; In conductometric enzymatic biosensors, enzymatic reaction is confined close to the interdigitated electrode surface, because enzyme is cross-linked in contact with this surface in the presence or absence of nanoparticles. The effect of the use of a new type of doubly-functionalized gold nanoparticles (PF-HEG-Au NPs) on the response of conductometric biosensor based on interdigitated electrodes (IDEs), for the detection of enzymatic substrates was studied. Gold nanoparticles (AuNPs) were first synthesized following the citrate process, with an average diameter of 14 nm. AuNPs were then functionalized with 11-mercaptoundecylhexaethyleneglycol (HEG) and then with 1H,1H,2H,2H-perfluorodecanethiol (PF). The doubly-functionalized AuNPs were characterized using TEM, UV-Vis spectrophotometry and FTIR spectroscopy. Urease, mixed with these doubly functionalized AuNPs, was then cross-linked with glutaraldhedyde vapor on the IDE surface. In the presence of urea, the conductometric response was measured in a differential mode. The best sensitivities for urea detection were obtained with PF-HEG-Au NPs (520 µS /mM and 0.5µM of detection limit), as compared to 284µS/mM and 2µM of detection limit with bare Au NPs, PF-AuNPs and HEG-AuNPs, and 1.07µS/mM and 100 µM of detection limit with urease directly crosslinked on IDEs.When stored in phosphate buffer (5 mM, pH 6.7) at 4 °C, the biosensor with PF-HEG-Au NPs showed good stability for more than 12 days.
Published: 2016

25. Molecularly imprinted polymer-based electrochemical sensor for the sensitive detection of glyphosate herbicide

Author: Minh Huy Do, Anca Florea, Francis Vocanson, Tran-Thi Nhu-Trang, François Bessueille, Carole Farre, Nicole Jaffrezic-Renault, Anne Bonhomme, Dept Analyt Chem, VNUHCM Univ Sci, Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Surfaces, Laboratoire Hubert Curien [Saint Etienne] (LHC), Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), and This work was supported by European Social Fund, Human Resources Development Operational Programme 2007-2013 [project number POSDRU/159/1.5/S/136893].
Subjects: EXTRACTION, animal structures, Health, Toxicology and Mutagenesis, GAS-CHROMATOGRAPHY, Soil Science, 02 engineering and technology, 01 natural sciences, metal organic framework, Analytical Chemistry, chemistry.chemical_compound, Aniline, glyphosate, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, METAL-ORGANIC FRAMEWORK, Environmental Chemistry, Molecule, WATER, Electrochemical sensors, Aminomethylphosphonic acid, Waste Management and Disposal, Water Science and Technology, Chromatography, FLUORESCENCE DETECTION, integumentary system, 010401 analytical chemistry, Public Health, Environmental and Occupational Health, Molecularly imprinted polymer, MASS-SPECTROMETRY, 021001 nanoscience & nanotechnology, Pollution, Combinatorial chemistry, PERFORMANCE LIQUID-CHROMATOGRAPHY, 0104 chemical sciences, Electrochemical gas sensor, chemistry, Colloidal gold, gold nanoparticles, Linear sweep voltammetry, embryonic structures, RESIDUES, molecularly imprinted polymers, AMINOMETHYLPHOSPHONIC ACID, 0210 nano-technology, Selectivity
Abstract: International audience; A sensitive electrochemical molecularly imprinted sensor was developed for the detection of glyphosate (Gly), by electropolymerisation of p-aminothiophenol-functionalised gold nanoparticles in the presence of Gly as template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognise and bind Gly through hydrogen bonds between Gly molecules and aniline moieties. The performance of the developed sensor for the detection of Gly was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as redox probe, the electron transfer rate increasing when concentration of Gly increases, due to a p-doping effect. The molecularly imprinted sensor exhibits a broad linear range, between 1 pg/L and 1 mu g/L and a quantification limit of 0.8 pg/L. The selectivity of the proposed sensor was investigated towards the binding of Gly metabolite, aminomethylphosphonic acid, revealing excellent selectivity towards Gly. The developed sensor was successfully applied to detect Gly in tap water samples.
Published: 2015
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26. Methylene blue phosphoramidite for DNA labelling

Author: Monique Sigaud, Corinne Sanglar, Gabriel De Crozals, Philippe Fortgang, Carole Chaix, Carole Farre, Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Micro & Nanobiotechnologies, and Lyon Science Transfert-Universite de Lyon and the French Ministry of Research for their financial support.
Subjects: SALTS, Photochemistry, SEQUENCE, Catalysis, OLIGONUCLEOTIDES, chemistry.chemical_compound, Electron transfer, PHOTODYNAMIC THERAPY, Organophosphorus Compounds, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Labelling, Materials Chemistry, Molecule, ELECTRON-TRANSFER, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Solid-Phase Synthesis Techniques, Phosphoramidite, ELECTROCHEMICAL HYBRIDIZATION INDICATOR, Oligonucleotide, Metals and Alloys, SENSOR, Electrochemical Techniques, General Chemistry, GOLD ELECTRODES, Combinatorial chemistry, 3. Good health, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Methylene Blue, chemistry, Ceramics and Composites, PHENOTHIAZINIUM PHOTOSENSITIZERS, Derivative (chemistry), Methylene blue, DNA, STEM-LOOP PROBE
Abstract: We thank Oceane Clabaux and Alexandre Escoffier for their contribution to the work.; International audience; We report the first synthesis of a methylene blue (MB) phosphoramidite derivative suitable for DNA solid-phase synthesis. The electrochemical and optical properties of the resulting MB modified oligonucleotides were confirmed. This new molecule is an important breakthrough in the design of new probes labelled with MB.
Published: 2015
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27. One-Step Fabrication of Electrospun Photo-Cross-Linkable Polymer Nanofibers Incorporating Multiwall Carbon Nanotubes and Enzyme for Biosensing

Author: Madjid Arab, Carole Farre, Mohamed Braiek, Florence Lagarde, Eleni Sapountzi, Jean-François Chateaux, Nicole Jaffrezic-Renault, Interfaces & biosensors - Interfaces & biocapteurs, Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Interfaces et Matériaux Avancés [Monastir] (LIMA), Faculté des Sciences de Monastir (FSM), Université de Monastir - University of Monastir (UM)-Université de Monastir - University of Monastir (UM), Institut des Matériaux, de Microélectronique et des Nanosciences de Provence (IM2NP), Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), INL - Lab-On-Chip et Instrumentation (INL - LOCI), Institut des Nanotechnologies de Lyon (INL), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Lyon (ECL), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), the authors thank the French government for the financial support., Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Lyon (ECL), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)
Subjects: COMPOSITE NANOFIBERS, GLUCOSE-OXIDASE, Materials science, POLY(VINYL ALCOHOL), Nanotechnology, Carbon nanotube, MEMBRANES, Polyvinyl alcohol, law.invention, chemistry.chemical_compound, DISPERSION, law, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Materials Chemistry, Electrochemistry, SENSORS, Glucose oxidase, biology, Renewable Energy, Sustainability and the Environment, MECHANICAL-PROPERTIES, [CHIM.MATE]Chemical Sciences/Material chemistry, PERFORMANCE, Condensed Matter Physics, Electrospinning, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Dielectric spectroscopy, Chemical engineering, chemistry, IMMOBILIZATION, Nanofiber, biology.protein, Cyclic voltammetry, Biosensor, FIBERS
Abstract: The authors thank INL institute (Institut des Nanotechnologies de Lyon, France) for providing the facilities for the electrospinning technique, the required access to the SEM facilities and for their remarkable assistance whenever it was needed. The Centre Technologique des Microstructures of Lyon 1 University is also acknowledged for providing the authors access to its TEM facilities. Finally, the authors thank the French government for the financial support.; International audience; Herein, we report the easy and rapid fabrication of water-stable electrospun nanofibers from blends of the photochemically cross-linkable polyvinyl alcohol styrylpyridinium polymer (PVA-SbQ), carboxylated multiwall carbon nanotubes (MWCNT-COOHs) and gluccise oxidase (GOx) for electrochemical biosensor application. Different electrospinning parameters including flow rate, applied voltage, distance between tip and collector, polymer concentration and MWCNT-COOHs content in the electrospun solution were tailored to produce PVA-SbQ/MWCNT-COOH nanofibers with minimal beading and enhanced electrical properties. Optimal PVA-SbQ and MWCNT-COOHs concentrations were 6.7 wt% and 5 wt% with respect to the total solution and polymer mass, respectively. The nanofibers were rendered water insoluble via exposure to UV irradiation for 10 mm. The electrochemical properties of electrospun PVA-SbQ/MWCNT-COOH nanofibrous mats were characterized by cyclic voltammetry and electrochemical impedance spectroscopy. Scanning electron microscopy and transmission electron microscopy were used to characterize the morphology of the nanofibers. G0x, used as model enzyme, was further incorporated into the PVA-SbQ/MWCNT-COOH mixture before electrospinning. The obtained biosensor enabled successful detection of glucose by cyclic voltammetry. The resulting novel glucose biosensor revealed very good storage and operational stabilities, a low limit of detection (2 mu M) and a wide linear range (up to at least 4 mM).
Published: 2015
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28. Development of innovative and versatile polythiol probes for use on ELOSA or electrochemical biosensors: application in hepatitis C virus genotyping

Author: Myriam Lereau, Julie Mayen, Carole Chaix, Jean-Jacques Vasseur, Chantal Fournier-Wirth, Carole Farre, Albert Meyer, Jean-François Cantaloube, François Morvan, Vincent Dugas, Laboratoire TransDiag-Sécurité Transfusionnelle et Innovation Diagnostique, Etablissement Français du Sang Pyrénées-Méditerranée, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), TECHSEP - TECHniques de SEParations (2011-2014), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Etablissement Français du Sang - Alpes-Méditerranée (EFS - Alpes-Méditerranée), Etablissement Français du Sang, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), ANR-09-PIRI-0023 VirProbe, LyonBio-Pole, Eurobiomed, and ANR-09-PIRI-0023,VirProbe,Conception d'un microsystème basé sur des sondes électrochimiques ultra-sensibles pour une multi-détection sans marquage d'acides nucléiques: application au génotypage HCV(2009)
Subjects: Genotype, Polymers, Hepatitis C virus, RT-PCR, Computational biology, Biosensing Techniques, Hepacivirus, 010402 general chemistry, medicine.disease_cause, Real-Time Polymerase Chain Reaction, 01 natural sciences, Analytical Chemistry, 03 medical and health sciences, chemistry.chemical_compound, optical enzyme-linked oligosorbent assay, medicine, Electrochemical biosensor, Humans, Sulfhydryl Compounds, Genotyping, NS5B, 030304 developmental biology, Detection limit, 0303 health sciences, Molecular Structure, Chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, real-time polymease chain reaction, Electrochemical Techniques, Amplicon, Molecular biology, NS5b, 0104 chemical sciences, ELOSA, HCV, Differential pulse voltammetry, Oligonucleotide Probes, Biosensor
Abstract: International audience; The aim of this study was to develop versatile diagnostic tools based on the use of innovative polythiolated probes for the detection of multiple viruses. This approach is compatible with optical enzyme-linked oligosorbent assay (ELOSA) or electrochemical (biosensors) detection methods. The application targeted here concerns the rapid genotyping of Hepatitis C virus (HCV). HCV genotyping is one of the predictive parameters currently used to define the antiviral treatment strategy and is based on the sequencing of the viral NS5b region. Generic and specific NS5b amplicons were produced by real-time polymease chain reaction (RT-PCR) on HCV(+) human plasma. Original NS5b probes were designed for genotypes 1a/1b, 2a/2b/2c, 3a, and 4a/4d. Robust polythiolated probes were anchored with good efficacy on maleimide-activated microplates (MAM) and gold electrodes. Their grafting on MAM greatly increased the sensitivity of the ELOSA test which was able to detect HCV amplicons with good sensitivity (10 nM) and specificity. Moreover, the direct and real-time electrochemical detection by differential pulse voltammetry enabled a detection limit of 10 fM to be reached with good reproducibility. These innovative polythiolated probes have allowed us to envisage developing flexible, highly sensitive, and easy-to-handle platforms dedicated to the rapid screening and genotyping of a wide range of viral agents.
Published: 2013
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29. Nanoparticules fluorescentes ciblantes pour l'imagerie médicale

Author: Grégoire Hantier, Gabriel de Crozals, Carole Farre, Bonazza, P., Claire Billotey, Marc Janier, Jean-Jacques Toulmé, Marquette, Christophe A., Cédric Louis, Carole Chaix, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physico-Chimie des Matériaux Luminescents (LPCML), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie (IECB), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Nano-H SAS, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes, Institut des Sciences Analytiques ( ISA ), École normale supérieure - Lyon ( ENS Lyon ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ) -École normale supérieure - Lyon ( ENS Lyon ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Physico-Chimie des Matériaux Luminescents ( LPCML ), Université Claude Bernard Lyon 1 ( UCBL ), Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie ( IECB ), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique ( CNRS ), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires ( ICBMS ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique ( CNRS ), and Bussy, Agnès
Subjects: [CHIM.ANAL] Chemical Sciences/Analytical chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [ CHIM.ANAL ] Chemical Sciences/Analytical chemistry, ComputingMilieux_MISCELLANEOUS
Abstract: communication par affiche; National audience
Published: 2012

30. Oligonucleotide solid-phase synthesis on fluorescent silica particles of nanometric size

Author: Gabriel de Crozals, Carole Farre, Marquette, Christophe A., Mandon, Céline A., Matteo Martini, Jean-Jacques Toulmé, Darocha, S., Claire Billotey, Marc Janier, Laurence Marmuse, Cédric Louis, Carole Chaix, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes, Institut des Sciences Analytiques ( ISA ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Lyon-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-École normale supérieure - Lyon ( ENS Lyon ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Lyon-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-École normale supérieure - Lyon ( ENS Lyon ), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires ( ICBMS ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Physico-Chimie des Matériaux Luminescents ( LPCML ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie ( IECB ), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique ( CNRS ), Nano-H SAS, Bussy, Agnès, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physico-Chimie des Matériaux Luminescents (LPCML), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie (IECB), and Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS)
Subjects: [CHIM.ANAL] Chemical Sciences/Analytical chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [ CHIM.ANAL ] Chemical Sciences/Analytical chemistry
Published: 2012

31. A sensitive and selective thrombin impedimetric aptasensor based on tailored aptamers obtained by solid-phase synthesis

Author: R. Kherrat, Nicole Jaffrezic-Renault, N. Meini, Sergei V. Dzyadevych, Carole Chaix, Carole Farre, Laboratoire Génie de l'Environnement, Université Badji Mokhtar - Annaba [Annaba] (UBMA), SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratory of Biomolecular Electrochemistry, National Academy of Sciences of Ukraine (NASU), AUF ( PhD thesis grant), EC (NANOBIOSENS Project PIRSES-GA-2008-2300802), NATO project CBP.NUKR.CLG 984221, European Project, Université Badji Mokhtar Annaba (UBMA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Aptamer, PROTEINS, Phosphoramidite synthons, Nanotechnology, 02 engineering and technology, 01 natural sciences, Solid-phase synthesis, Thrombin, BIOSENSOR, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Materials Chemistry, medicine, SURFACE-PLASMON RESONANCE, ASSAY, Electrical and Electronic Engineering, Surface plasmon resonance, Instrumentation, Detection limit, Phosphoramidite, Chemistry, 010401 analytical chemistry, Metals and Alloys, Aptasensor, DNA, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Dielectric spectroscopy, IMPEDANCE SPECTROSCOPY DETECTION, LIGANDS, 0210 nano-technology, Biosensor, Electrochemical impedance spectroscopy, medicine.drug
Abstract: International audience; The aim of the present work is to show the interest of a generic concept of aptamers directly obtained through a supported synthesis, using phosphoramidite synthons, for biosensor application. By that way, we were able to tailor the anchoring groups and specific spacers. The concept is validated on an impedimetric aptasensor for detection of thrombin. Two aptamer sequences, selected from literature (15 and 29 mers), were synthesized with a multi-thiol modification at one extremity. After their grafting on gold electrode, the faradic electrochemical impedance measurements allow the direct detection of thrombin in the range of 3-30 ng mL(-1) ancl 3-50 ng mL(- 1) for 15 meraptamer and 29 meraptamer respectively, with a detection limit of 3.1 ng mL(-1) (80 pmol/l) for both sequences. This study demonstrates that these aptasensors exhibit high sensitivity (higher sensitivity for 29 meraptamer), high selectivity (compared to a model protein, BSA), stability and reproducibility
Published: 2012
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32. Oligonucleotide solid-phase synthesis on fluorescent nanoparticles grafted on controlled pore glass

Author: Carole Chaix, Carole Farre, Grégoire Hantier, Jean-Jacques Toulmé, Marc Janier, Claire Billotey, Didier Léonard, Christophe A. Marquette, Cédric Louis, Gabriel De Crozals, Laurence Marmuse, Céline A. Mandon, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes, Institut des Sciences Analytiques ( ISA ), École normale supérieure - Lyon ( ENS Lyon ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ) -École normale supérieure - Lyon ( ENS Lyon ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires ( ICBMS ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Nano-H SAS, Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie ( IECB ), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Physico-Chimie des Matériaux Luminescents ( LPCML ), ANR (ANR-08-Biotecs-003), LyonBiopole, Lyon Science Transfert, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie (IECB), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physico-Chimie des Matériaux Luminescents (LPCML), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon
Subjects: Materials science, General Chemical Engineering, Nanoparticle, Nanotechnology, BIOANALYSIS, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, chemistry.chemical_compound, Solid-phase synthesis, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, OXIDE NANOPARTICLES, Phosphoramidite, DNA synthesis, Oligonucleotide, General Chemistry, DNA, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Chemical engineering, chemistry, IMMOBILIZATION, Colloidal gold, GOLD NANOPARTICLES, Surface modification, FUNCTIONALIZATION, [ CHIM.ANAL ] Chemical Sciences/Analytical chemistry, 0210 nano-technology
Abstract: International audience; Oligonucleotide solid-phase synthesis is now possible on nano-sized particles, thanks to the use of controlled pore glass-nanoparticle assemblies. We succeeded in anchoring silica nanoparticles (NPs) inside the pores of micrometric glass via a reversible covalent binding. The pore diameter must be at least six times the diameter of the nanoparticle in order to maintain efficient synthesis of oligonucleotides in the synthesizer. We demonstrated that the pores protect NP anchoring during DNA synthesis without decreasing the coupling rate of the phosphoramidite synthons. This bottom-up strategy for NP functionalization with DNA results in unprecedented DNA loading efficiency. We also confirmed that the DNA synthesized on the nanoparticle surface was accessible for hybridization with its complementary DNA strand.
Published: 2012
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33. A 'four-ferrocene' modified stem-loop structure as a probe for sensitive detection and single-base mismatch discrimination of DNA

Author: Carole Chaix, Carole Farre, Grégory Chatelain, Salomé Ansanay-Alex, Micaël Ripert, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)
Subjects: Detection limit, Electrochemical transduction, 010405 organic chemistry, Oligonucleotide, General Chemical Engineering, Analytical chemistry, DNA, 010402 general chemistry, 01 natural sciences, Genosensor, 0104 chemical sciences, chemistry.chemical_compound, Ferrocene, chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Electrode, Electrochemistry, Stem-loop structure, Differential pulse voltammetry, Cyclic voltammetry, Biochip, Biosensor
Abstract: International audience; We report the use of a four-ferrocene modified oligonucleotide as a probe for DNA detection with a gold electrode microsystem. This oligonucleotide is synthesized by automated solid-phase synthesis with four successive ferrocene moieties at the 5′-end and a C6-thiol modifier group at the 3′-end. The grafting of this 4Fc-DNA probe on a gold electrode microsystem results in the appearance of the ferrocene redox couple in cyclic voltammetry. The probe sequence is a stem-loop structure that folds efficiently on the electrode, thus optimizing electron transfer. Such architecture serves as sensor for DNA detection which is based on hybridization. The resulting disposable voltammetric sensor allowed direct, reagentless DNA detection in a small volume (20 μL). Electrochemical response upon hybridization with complementary short sequence (30-base length) and long sequence (50-base length) strands was observed by differential pulse voltammetry. Current variations were compared. The longer the sequence, the greater the decrease in current. The system's detection limit was estimated at 3.5 pM (0.07 fmol in 20 μL) with the 50-base length target and provided a dynamic detection range between 3.5 pM and 5 nM. Single mismatch detection showed a good level of sensitivity. The system was regenerated twice with no significant loss of Fc signal. Finally, 1 pM sensitivity was reached with a long chain analog of DNA PCR products of Influenza virus.
Published: 2012
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34. Impedimetric aptasensor for thrombin detection

Author: Nicole Jaffrezic-Renault, N. Meini, Carole Farre, K. Kherrat, Sergei V. Dzyadevych, Carole Chaix, Université Badji Mokhtar - Annaba [Annaba] (UBMA), SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes (2011-2014), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratory of Biomolecular Electrochemistry, and National Academy of Sciences of Ukraine (NASU)
Subjects: Aptamer, High selectivity, Analytical chemistry, 02 engineering and technology, 01 natural sciences, Thrombin, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, medicine, aptasensor, Engineering(all), Detection limit, Reproducibility, Chromatography, Chemistry, 010401 analytical chemistry, Model protein, aptamer, electrochemical impedance spectroscopy (EIS), General Medicine, 021001 nanoscience & nanotechnology, thrombin, 0104 chemical sciences, Electrode, 0210 nano-technology, [CHIM.OTHE]Chemical Sciences/Other, medicine.drug
Abstract: International audience; The goal of this work is to develop an impedimetric aptasensor sensitive, selective and stable for detection of thrombin. Thiol modified aptamer sequence being grafted on gold electrode, the faradic electrochemical impedance measurements allow the direct detection of thrombin in the range of 3.1-200 ngmL(-1) with a detection limit of 3.1 ngmL(-1) (80 fM). This study demonstrates that these aptasensors exhibit high sensitivity, high selectivity (compared to a model protein, BSA), stability and reproducibility.
Published: 2011
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35. Automated oligonucleotide solid-phase synthesis on nanosized silica particles using nano-on-micro assembled particle supports

Author: Gaëlle Catanante, Stéphane Roux, Christophe A. Marquette, Cédric Louis, Carole Chaix, Loïc J. Blum, Carole Farre, Muriel Lansalot, Nicolas Charvet, Rana Bazzi, Laboratoire de chimie et procédés de polymérisation (LCPP), École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Département Economie et Sociologie des Transports (INRETS/DEST), Institut National de Recherche sur les Transports et leur Sécurité (INRETS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institute of Molecular Biology and Biotechnology (IMBB-FORTH), Foundation for Research and Technology - Hellas (FORTH), Department of Biology, University of Crete [Heraklion] (UOC), Sciences Analytiques (SA), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), and Depierre, Frédérique
Subjects: Scanning electron microscope, Oligonucleotides, Binary compound, Nanoparticle, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, chemistry.chemical_compound, Solid-phase synthesis, Microscopy, Electron, Transmission, Nano, Scanning transmission electron microscopy, Electrochemistry, General Materials Science, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Spectroscopy, Surfaces and Interfaces, Models, Theoretical, respiratory system, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Silicon Dioxide, 0104 chemical sciences, chemistry, Oligodeoxyribonucleotides, Transmission electron microscopy, Microscopy, Electron, Scanning, Particle, Nanoparticles, 0210 nano-technology
Abstract: International audience; This article describes an original strategy to enable solid-phase oligodeoxyribonucleotide (ODN) synthesis on nanosized silica particles. It consists of the reversible immobilization of silica nanoparticles (NPs) on micrometric silica beads. The resulting assemblies, called nano-on-micro (NOM) systems, are well adapted to ODN synthesis in an automated instrument. First, NPs are derivatized with OH functions. For NOM assembly preparation, these functions react with the silanols of the microbeads under specific experimental conditions. Furthermore, OH groups allow ODN synthesis on the nanoparticles via phosphoramidite chemistry. The stability of the NOM assemblies during ODN solid-phase synthesis is confirmed by scanning and transmission electron microscopy (SEM and TEM, respectively), together with dynamic light scattering analyses. Then, the release of ODN-functionalized nanoparticles is performed under mild conditions (1% NH4OH in water, 1 h, 60 °C). Our technique provides silica nanoparticles well functionalized with oligonucleotides, as demonstrated by hybridization experiments conducted with the cDNA target.
Published: 2010

36. A one-step derivatization of silica supports with various hydroxylated compounds (reporter molecules, nanoparticles) for oligonucleotide solid-phase synthesis

Author: Carole Chaix, Stéphane Roux, Muriel Lansalot, Christophe A. Marquette, Cédric Louis, Rana Bazzi, Carole Farre, Nicolas Charvet, Alain Laurent, and Loïc J. Blum
Subjects: chemistry.chemical_compound, Solid-phase synthesis, chemistry, Oligonucleotide, Nanoparticle, Organic chemistry, Molecule, One-Step, Derivatization, Combinatorial chemistry
Published: 2008
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37. Are we mis-estimating chemotherapy-induced peripheral neuropathy? Analysis of assessment methodologies from a prospective, multinational, longitudinal cohort study of patients receiving neurotoxic chemotherapy

Author: Alex Molassiotis, Hui Lin Cheng, Violeta Lopez, Joseph S. K. Au, Alexandre Chan, Aishwarya Bandla, K. T. Leung, Y. C. Li, K. H. Wong, Lorna K. P. Suen, Choi Wan Chan, Janelle Yorke, Carole Farrell, and Raghav Sundar
Subjects: Peripheral neuropathy, Neurotoxicity, Chemotherapy, Cancer, Assessment, Taxanes, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract: Abstract Background There are inconsistencies in the literature regarding the prevalence and assessment of chemotherapy-induced peripheral neuropathy (CIPN). This study explored CIPN natural history and its characteristics in patients receiving taxane- and platinum-based chemotherapy. Patients and methods Multi-country multisite prospective longitudinal observational study. Patients were assessed before commencing and three weekly during chemotherapy for up to six cycles, and at 6,9, and 12 months using clinician-based scales (NCI-CTCAE; WHO-CIPN criterion), objective assessments (cotton wool test;10 g monofilament); patient-reported outcome measures (FACT/GOG-Ntx; EORTC-CIPN20), and Nerve Conduction Studies. Results In total, 343 patients were recruited in the cohort, providing 2399 observations. There was wide variation in CIPN prevalence rates using different assessments (14.2–53.4%). Prevalence of sensory neuropathy (and associated symptom profile) was also different in each type of chemotherapy, with paclitaxel (up to 63%) and oxaliplatin (up to 71.4%) showing the highest CIPN rates in most assessments and a more complex symptom profile. Peak prevalence was around the 6-month assessment (up to 71.4%). Motor neurotoxicity was common, particularly in the docetaxel subgroup (up to 22.1%; detected by NCI-CTCAE). There were relatively moderately-to-low correlations between scales (rs = 0.15,p
Published: 2019
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