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3 results on '"Carole Capitaine"'

1. Comparisons of liver proteomes in the European flounder Platichthys flesus from three contrasted estuaries

Author

Michel Auffret, Vianney Pichereau, Louis Quiniou, Claire Galland, Cécile Dupuy, Carole Capitaine, Jean Laroche, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Sentinel species, Platichthys flesus, Vitelline membrane, Liver Proteomics, 010501 environmental sciences, Aquatic Science, Biology, Oceanography, 01 natural sciences, 03 medical and health sciences, Juvenile, 14. Life underwater, EUROPEAN FLOUNDER, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, 0105 earth and related environmental sciences, 0303 health sciences, geography, geography.geographical_feature_category, Ecology, Estuary, Biomarker, biology.organism_classification, Pollution, Platichthys, [SDE]Environmental Sciences, Proteome
Abstract

International audience; Estuaries are important areas highly susceptible to anthropogenic degradations like pollution. Estuarine species have thus to cope with many types of constraints depending on the estuaries' characteristics. The European flounder Platichthys flesus is considered as a sentinel species for the monitoring of estuarine water quality. In this study, juvenile flounders (0+ group) were sampled from three contrasted Channel estuaries, i.e. the Seine, the Canche and the Tamar, and we characterized their liver proteomes by using a two-dimensional electrophoresis based proteomic approach. We showed that 27 protein spots differentially accumulated between the 3 populations. Six of these proteins were identified by MALDI TOF-TOF mass spectrometry. Flounders from the Seine and from the Tamar, two highly polluted estuaries, displayed common differences, i.e. an increase of the energetic- and the glutathione-metabolism. The most accumulated protein in the Seine's samples (6.7-fold) was a Vitelline Membrane Outer layer protein 1 homolog, suggesting oogenesis deregulation in these juvenile (sexually immature) flounders. Future works applying this kind of proteomic approach on flounders experimentally exposed to conditions that mimic environmental constraints will help to better understand the significance of these environmental proteomic signatures.

Published
2013
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2. Vibrio harveyi Adheres to and Penetrates Tissues of the European Abalone Haliotis tuberculata within the First Hours of Contact

Author

Antoine M. Dujon, Christine Paillard, Carole Capitaine, Annaïck Barbou, Adeline Bidault, Dario Moraga, Marion Cardinaud, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Gills, Gill, Time Factors, Hypobranchial gland, food.ingredient, Abalone, [SDV]Life Sciences [q-bio], Gastropoda, Green Fluorescent Proteins, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Applied Microbiology and Biotechnology, Microbiology, food, Hemolymph, Vibrio Infections, Invertebrate Microbiology, Animals, 14. Life underwater, Haliotis, Vibrio, Ecology, biology, Vibrio harveyi, ACL, fungi, biology.organism_classification, 3. Good health, Host-Pathogen Interactions, bacteria, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Food Science, Biotechnology
Abstract

Vibrio harveyi is a marine bacterial pathogen responsible for episodic epidemics generally associated with massive mortalities in many marine organisms, including the European abalone Haliotis tuberculata . The aim of this study was to identify the portal of entry and the dynamics of infection of V. harveyi in the European abalone. The results indicate that the duration of contact between V. harveyi and the European abalone influences the mortality rate and precocity. Immediately after contact, the epithelial and mucosal area situated between the gills and the hypobranchial gland was colonized by V. harveyi . Real-time PCR analyses and culture quantification of a green fluorescent protein-tagged strain of V. harveyi in abalone tissues revealed a high density of bacteria adhering to and then penetrating the whole gill-hypobranchial gland tissue after 1 h of contact. V. harveyi was also detected in the hemolymph of a significant number of European abalones after 3 h of contact. In conclusion, this article shows that a TaqMan real-time PCR assay is a powerful and useful technique for the detection of a marine pathogen such as V. harveyi in mollusk tissue and for the study of its infection dynamics. Thus, we have revealed that the adhesion and then the penetration of V. harveyi in European abalone organs begin in the first hours of contact. We also hypothesize that the portal of entry of V. harveyi in the European abalone is the area situated between the gills and the hypobranchial gland.

Published
2014
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3. Assessment of the European flounder responses to chemical stress in the English Channel, considering biomarkers and life history traits

Author

Michel Fournier, Claire Galland, Jean Laroche, Maylis Labonne, Rachid Amara, Ricardo Riso, Grégory Charrier, Vianney Pichereau, Célie Dupuy, Wilfried Sanchez, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique [Québec] (INRS)-Réseau International des Instituts Pasteur (RIIP), Institut National de l'Environnement Industriel et des Risques (INERIS), Institut de Recherche pour le Développement (IRD [France-Ouest]), Université Lille Nord de France (COMUE), Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG), Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), This study was supported firstly by the EEC INTERREG IV program: DIESE (50% of a PhD grant was obtained by the first author, for the development of immune markers in ecotoxicology), and secondly by the DEVIL-INERIS program and by the EVOLFISH project (ANR-VMCS). Financial support was also provided by the Canada Research Chair in Environmental Immunotoxicology (Dr. Michel Fournier) and Collège Doctoral International de l’UniversitéEuropéenne de Bretagne. Authors thank Carole Capitaine for her excellent technical assistance, and also wish to thank Dr. W. Langston for fish sampling in the Laboratory of the Marine Biological Association, in Plymouth, ANR-08-VULN-0004,EVOLFISH,Effects of global warming and contamination on the evolutionnary response of two coastal fishes(2008), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Nord])

Subjects
Pollution, media_common.quotation_subject, [SDV]Life Sciences [q-bio], Context (language use), Flounder, Aquatic Science, Oceanography, Life history theory, Life-history traits, Metals, Heavy, medicine, Animals, Immunotoxicity, 14. Life underwater, EUROPEAN FLOUNDER, Polycyclic Aromatic Hydrocarbons, Ecosystem, Otolith, media_common, Life Cycle Stages, geography, geography.geographical_feature_category, biology, Ecology, ACL, Estuary, biology.organism_classification, Platichthys, Up-Regulation, Europe, medicine.anatomical_structure, Fish, Gene expression, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Estuaries, Biomarkers, Water Pollutants, Chemical, Environmental Monitoring
Abstract

International audience; A multi-biomarker approach was developed to evaluate responses of European flounder (Platichthys flesus) in three contrasted estuaries over the English Channel: the Canche (pristine site), Tamar (heavy metals and PAHs contamination) and Seine (heavily pollution with a complex cocktail of contaminants). The condition factor and several biomarkers of the immune system, antioxidant enzymes, energetic metabolism and detoxification processes were investigated in young-of-the-year (0+) and one-year-old (1+) flounder. Results underlined the difference between the pristine site and the Seine estuary which showed a lower condition factor, a modulation of the immune system, a higher Cytochrome C oxidase activity, and an up-regulation of BHMT expression. The moderate biomarker responses in the Tamar fish could be linked to the specific contamination context of this estuary. Flounder life history traits were analyzed by otolith microchemistry, in order to depict how the fish use their habitat and thus respond to chemical stress in estuaries.

Published
2015
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