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16 results on '"Caro, Tristan"'

1. Growth rate as a link between microbial diversity and soil biogeochemistry

Author

Foley, Megan M., Stone, Bram W. G., Caro, Tristan A., Sokol, Noah W., Koch, Benjamin J., Blazewicz, Steven J., Dijkstra, Paul, Hayer, Michaela, Hofmockel, Kirsten, Finley, Brianna K., Mack, Michelle, Marks, Jane, Mau, Rebecca L., Monsaint-Queeney, Victoria, Morrissey, Ember, Propster, Jeffrey, Purcell, Alicia, Schwartz, Egbert, Pett-Ridge, Jennifer, Fierer, Noah, and Hungate, Bruce A.

Published
2024
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2. Single-cell measurement of microbial growth rate with Raman microspectroscopy.

Author

Caro, Tristan A, Kashyap, Srishti, Brown, George, Chen, Claudia, Kopf, Sebastian H, and Templeton, Alexis S

Subjects
*SECONDARY ion mass spectrometry, *STABLE isotope analysis, *MICROBIAL growth, *BIOMASS production, *RAMAN spectroscopy
Abstract

Rates of microbial growth are fundamental to understanding environmental geochemistry and ecology. However, measuring the heterogeneity of microbial activity at the single-cell level, especially within complex populations and environmental matrices, remains a forefront challenge. Stable isotope probing (SIP) is a method for assessing microbial growth and involves measuring the incorporation of an isotopic label into microbial biomass. Here, we assess Raman microspectroscopy as a SIP technique, specifically focusing on the measurement of deuterium (2H), a tracer of microbial biomass production. We correlatively measured cells grown in varying concentrations of deuterated water with both Raman spectroscopy and nanoscale secondary ion mass spectrometry (nanoSIMS), generating isotopic calibrations of microbial 2H. Relative to Raman, we find that nanoSIMS measurements of 2H are subject to substantial dilution due to rapid exchange of H during sample washing. We apply our Raman-derived calibration to a numerical model of microbial growth, explicitly parameterizing the factors controlling growth rate quantification and demonstrating that Raman–SIP can sensitively measure the growth of microorganisms with doubling times ranging from hours to years. The measurement of single-cell growth with Raman spectroscopy, a rapid, nondestructive technique, represents an important step toward application of single-cell analysis into complex sample matrices or cellular assemblages. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Chapter 8: Searching for Life Beyond Earth

Author

Chou, Luoth, primary, Grefenstette, Natalie, additional, Borges, Schuyler, additional, Caro, Tristan, additional, Catalano, Enrico, additional, Harman, Chester E., additional, McKaig, Jordan, additional, Raj, Chinmayee Govinda, additional, Trubl, Gareth, additional, and Young, Amber, additional

Published
2024
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4. Chapter 1: The Astrobiology Primer 3.0

Author

Schaible, Micah J., primary, Szeinbaum, Nadia, additional, Bozdag, G. Ozan, additional, Chou, Luoth, additional, Grefenstette, Natalie, additional, Colón-Santos, Stephanie, additional, Rodriguez, Laura E., additional, Styczinski, M.J., additional, Thweatt, Jennifer L., additional, Todd, Zoe R., additional, Vázquez-Salazar, Alberto, additional, Adams, Alyssa, additional, Araújo, M.N., additional, Altair, Thiago, additional, Borges, Schuyler, additional, Burton, Dana, additional, Campillo-Balderas, José Alberto, additional, Cangi, Eryn M., additional, Caro, Tristan, additional, Catalano, Enrico, additional, Chen, Kimberly, additional, Conlin, Peter L., additional, Cooper, Z.S., additional, Fisher, Theresa M., additional, Fos, Santiago Mestre, additional, Garcia, Amanda, additional, Glaser, D.M., additional, Harman, Chester E., additional, Hermis, Ninos Y., additional, Hooks, M., additional, Johnson-Finn, K., additional, Lehmer, Owen, additional, Hernández-Morales, Ricardo, additional, Hughson, Kynan H.G., additional, Jácome, Rodrigo, additional, Jia, Tony Z., additional, Marlow, Jeffrey J., additional, McKaig, Jordan, additional, Mierzejewski, Veronica, additional, Muñoz-Velasco, Israel, additional, Nural, Ceren, additional, Oliver, Gina C., additional, Penev, Petar I., additional, Raj, Chinmayee Govinda, additional, Roche, Tyler P., additional, Sabuda, Mary C., additional, Schaible, George A., additional, Sevgen, Serhat, additional, Sinhadc, Pritvik, additional, Steller, Luke H., additional, Stelmach, Kamil, additional, Tarnas, J., additional, Tavares, Frank, additional, Trubl, Gareth, additional, Vidaurri, Monica, additional, Vincent, Lena, additional, Weber, Jessica M., additional, Weng, Maggie Meiqi, additional, Wilpiszeki, Regina L., additional, and Young, Amber, additional

Published
2024
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11. In-space Biomanufacturing Using CO2 with Methylobacterium Extorquens

Author

Caro, Tristan, Stankiewiz, Jamie, Rahman, Asif, Hogan, John A, and Galazka, Jonathan M

Subjects
Life Sciences (General)
Abstract

Long duration missions to deep space will require new approaches for supplying astronauts. In-space microbial manufacturing could generate many important compounds (such as nutrients, pharmaceuticals and fuels) but there are significant barriers to deploying reliable bioproduction platforms to space. These include ensuring adequate production and proper purification of the desired product, especially in the unique radiation and microgravity environment. Here we are focused on developing methods and technologies to feed microbial factories using the resources available in space. CO2, found in abundance in spacecraft cabins and the Mars atmosphere, can be sequestered and converted into bioproducts. While autotrophic organisms can use CO2 directly, they are generally slow growing and have less-developed biotechnology toolkits. Therefore we are developing an alternative paradigm in which CO2 is first reduced to more energetic carbon compounds that can support more rapid growth of workhorse biotechnology platforms (E. coli, S. cerevisiae, P. pastoris).Various technologies exist or are being developed to convert CO2. For example, the Sabatier system currently installed on the ISS, reacts CO2 and H2 to generate CH4 and H2O. This methane could be consumed by engineered methanotrophic bacteria. Alternatively, electrochemical systems can convert CO2 into formate (CHO2) which could be consumed by formatotrophic bacteria. In either case, synthetic biology techniques allow these microbes to serve as reprogrammable biofactories capable of producing a vast number or products.

Published
2018

14. Ultraviolet light measurements (280–400 nm) acquired from stratospheric balloon flight to assess influence on bioaerosols

Author

Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences, Caro, Tristan A, Wendeln, Matthew, Freeland, Matthew, Bryan, Noelle, Waters, Samantha M, McIntyre, Alexa, Nicoll, Patrick, Madronich, Sasha, Smith, David J, Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences, Caro, Tristan A, Wendeln, Matthew, Freeland, Matthew, Bryan, Noelle, Waters, Samantha M, McIntyre, Alexa, Nicoll, Patrick, Madronich, Sasha, and Smith, David J

Abstract

Viable microorganisms collected from the Earth’s upper atmosphere are mysterious considering the intensely biocidal ultraviolet (UV) light conditions dominating rarefied air. Historically, most investigations examining the relationship between bioaerosols and UV conditions in the upper atmosphere have relied upon model-generated data. To address the shortage of in situ UV measurements in the upper troposphere and lower/middle stratosphere, we flew a meteorological balloon equipped with a UV radiometer and other core environmental sensors. The balloon payload launched from Illinois, USA, on October 6, 2018, and acquired UVA (315–400 nm) + UVB (280–315 nm) measurements for ~ 2 h up to 30.9 km. Above the atmospheric boundary layer, UVA + UVB values registered around 6 mW cm−2, results that were largely consistent with Tropospheric Ultraviolet–Visible model predictions. Performed in a low-cost, reusable manner with commercially available instruments, we show that reliable UV flux data can be acquired with meteorological balloon payload systems. This short communication provides relevant UVA + UVB results for aerobiology and astrobiology studies evaluating the survivability of microorganisms in the upper atmosphere.

Published
2021

15. On the Past, Present, and Future Role of Biology in NASA’s Exploration of our Solar System

Author

Hand, Kevin, primary, Phillips, Cynthia B., additional, Chyba, Christopher F., additional, Toner, Brandy, additional, Katija, Kakani, additional, Orphan, Victoria, additional, Huber, Julie, additional, Cavanaugh, Colleen M., additional, Carlson, Marian, additional, Christner, Brent, additional, Templeton, Alexis, additional, Seewald, Jeffrey, additional, Hofgartner, Jason D., additional, Amend, Jan P., additional, Orcutt, Beth N., additional, Bartlett, Douglas H., additional, Falkowski, Paul, additional, Anderson, Rika, additional, Spear, John R., additional, Shank, Tim, additional, Fischer, Woodward W., additional, Hazen, Robert M., additional, Hoehler, Tori, additional, D'Hondt, Steven, additional, Pitesky, Jo, additional, Lynch, Kennda, additional, Shock, Everett L., additional, Craft, Kate, additional, Boyd, Eric, additional, House, Christopher H., additional, Reysenbach, Anna-Louise, additional, Glass, Jennifer, additional, Fike, David, additional, Baross, John A., additional, Gogarten, Johann Peter, additional, Kaçar, Betül, additional, El-Naggar, Moh, additional, Murray, Alison E., additional, Dupont, Christopher, additional, Scully, Jennifer, additional, Rothschild, Lynn, additional, Trembath-Reichert, Elizabeth, additional, Klein, Frieder, additional, Cohen, Phoebe A., additional, Gile, Gillian H., additional, Lloyd, Karen, additional, Dekas, Anne, additional, Delaney, John R., additional, Skidmore, Mark, additional, Buongiorno, Joy, additional, Rogers, Karyn, additional, Hofmann, Amy, additional, Brazelton, William J., additional, Anbar, Ariel, additional, Manalang, Dana Ann, additional, Stevenson, Bradley, additional, Neuer, Susanne, additional, Hara, Ellie, additional, Nordheim, Thomas, additional, Shapiro, Russell, additional, Bradley, Alexander S., additional, Mikucki, Jill, additional, Brown, Michael E., additional, Glamoclija, Mihaela, additional, Reyes, Carolina, additional, Sánchez-Román, Mónica, additional, Farmer, Jack D., additional, Giovannelli, Donato, additional, Suel, Gurol, additional, Trumbo, Samantha, additional, Cameron, Marissa, additional, Osburn, Magdalena, additional, Bradley, James Andrew, additional, Garcia-Pichel, Ferran, additional, Steen, Andrew D., additional, Marlow, Jeffrey, additional, Trubl, Gareth, additional, Robinson, Kirtland, additional, Caro, Tristan, additional, Fulfer, Victoria, additional, Parker, Ceth W., additional, Feyhl-Buska, Jayme, additional, and Roussel, Anaïs, additional

Published
2021
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16. A High-Altitude Balloon Platform for Space Life Sciences Education

Author

McKaig, Jordan, primary, Caro, Tristan, additional, Hyer, Alex, additional, Talburt, Elizabeth Delgadillo, additional, Verma, Sonali, additional, Cui, Kaixin, additional, Boguraev, Anna-Sophia, additional, Heit, Molly, additional, Johnson, Aimee, additional, Johnson, Emily, additional, Jong, Andrew, additional, Shepard, Brooke, additional, Stankiewiz, Jamie, additional, Tran, Nhung, additional, and Rask, Jon, additional

Published
2019
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