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5 results on '"Carmen-Elena Arias"'

1. Differential cardiomyocyte transcriptomic remodeling during in vitro Trypanosoma cruzi infection using laboratory strains provides implications on pathogenic host responses

Author

Katherine-Sofia Candray-Medina, Yu Nakagama, Masamichi Ito, Shun Nakagama, Evariste Tshibangu-Kabamba, Norihiko Takeda, Yuki Sugiura, Yuko Nitahara, Yu Michimuko-Nagahara, Natsuko Kaku, Yoko Onizuka, Carmen-Elena Arias, Maricela Mejia, Karla Alas, Susana Peña, Yasuhiro Maejima, Issei Komuro, Junko Nakajima-Shimada, and Yasutoshi Kido

Subjects
Trypanosoma cruzi, Chagas disease, Transcriptome, HL-1, In vitro modeling, Dilated cardiomyopathy, Arctic medicine. Tropical medicine, RC955-962
Abstract

Abstract Background Chagas disease can lead to life-threatening cardiac manifestations. Regional factors, including genetic characteristics of circulating Trypanosoma cruzi (T. cruzi), have attracted attention as likely determinants of Chagas disease phenotypic expression and Chagas cardiomyopathy (CCM) progression. Our objective was to elucidate the differential transcriptomic signatures of cardiomyocytes resulting from infection with genetically discrete T. cruzi strains and explore their relationships with CCM pathogenesis and progression. Methods HL-1 rodent cardiomyocytes were infected with T. cruzi trypomastigotes of the Colombian, Y, or Tulahuen strain. RNA was serially isolated post-infection for microarray analysis. Enrichment analyses of differentially expressed genes (fold-change ≥ 2 or ≤ 0.5) highlighted over-represented biological pathways. Intracellular levels of reactive oxygen species (ROS) were compared between T. cruzi-infected and non-infected HL-1 cardiomyocytes. Results We found that oxidative stress-related gene ontology terms (GO terms), ‘Hypertrophy model’, ‘Apoptosis’, and ‘MAPK signaling’ pathways (all with P

Published
2023
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2. Re-emerging threat of Trypanosoma cruzi vector transmission in El Salvador, update from 2018 to 2020

Author

Marvin Stanley Rodríguez, Yuko Nitahara, Michelle Cornejo, Kevin Siliezar, Rafael Grande, Ana González, Kotaro Tasaki, Yu Nakagama, Yu Michimuko, Yoko Onizuka, Junko Nakajima-Shimada, José Eduardo Romero, José Ricardo Palacios, Carmen Elena Arias, William Mejía, Yasutoshi Kido, and Ricardo Cardona Alvarenga

Subjects
Chagas disease, Vector transmission, Triatomine, Trypanosoma cruzi, Triatoma dimidiata, Infectious and parasitic diseases, RC109-216, Public aspects of medicine, RA1-1270
Abstract

Abstract Background Since the late twentieth century, Chagas disease gained global attention to suppress the vector burden as a main control strategy in endemic countries. In Central America, multi-national initiative successfully achieved significant reduction in the estimated disease prevalence as well as elimination of the region’s principal vector species at the time in 2012. While the last decade has witnessed significant changes in ecosystem—such as urbanization and replacement of the main vector species—that can possibly affect the vector’s habitation and residual transmission, the up-to-date vector burden in the region has not been evaluated thoroughly due to the cessation of active vector surveillance. The aim of this study was to update the risk of vector-borne Trypanosoma cruzi infection in El Salvador, the top Chagas disease-endemic country in Central America. Methods A nationwide vector survey was conducted in the domestic environment of El Salvador from September 2018 to November 2020. The selection of the houses for inspection was based on expert purposeful sampling. Infection for T. cruzi was examined by microscopic observation of the insects’ feces, followed by a species confirmation using PCR. The data were analyzed using R software version 4.1.3. Proportion estimates with 95% confidence intervals were inferred using the Jeffrey’s method provided under the epiR package. Results A total of 1529 Triatoma dimidiata was captured from 107 houses (infestation rate, 34.4%; 107/311) in all the fourteen departments of the country visited within the period; prevalence of T. cruzi infection was as high as 10% (153/1529). In the country, domestic T. dimidiata infestation was distributed ubiquitously, while T. cruzi infection rates varied across the departments. Five out of fourteen departments showed higher infection rates than the average, suggesting sporadic high-risk areas in the country. Conclusions Our comprehensive study revealed substantial T. cruzi infection of T. dimidiata across the country, indicating potential active transmission of the disease. Therefore, strengthened surveillance for both vector and human infection is required to truly eliminate the risk of T. cruzi transmission in Central America.

Published
2022
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3. Constructo teórico para la gestión de proyectos de tecnología de información

Author

Christian Ronceros Morales and Carmen Elena Arias Delgado

Subjects
Automotive Engineering
Abstract

El objetivo de la presente investigación fue desarrollar un constructo teórico para la gestión de proyectos de tecnología de información (TI), tomándose como caso de estudio la Gerencia de AIT Región Oriente Sur perteneciente a Petróleos de Venezuela S.A. (PDVSA). El trabajo se enmarca dentro una investigación con modalidad proyecto factible, con un nivel de investigación descriptivo y de tipo de campo. Las técnicas de recolección de datos utilizadas fueron la revisión documental, la observación directa y las entrevistas no estructuradas. La muestra estuvo representada por los lideres de proyectos adscritos a la gerencia. El constructo teórico se encuentra soportado en una oficina de proyectos y en un modelo de madurez (basado en el OPM3 - Organizacional Project Management Maturity Model) en gestión de proyectos compuesta por cinco (5) niveles, desde el más bajo (nivel 0) hasta lograr el objetivo deseado (nivel 4), es decir, el mejoramiento continuo y el uso de las mejores prácticas. Este efecto permite identificar, implementar y optimizar las capacidades críticas de manera ordenada. El constructo teórico para la gestión de los proyectos TI provee una representación sencilla de este proceso, lo cual guía y facilita la ejecución de proyectos a los diferentes actores que intervienen en el mismo, asegurándose la comprensión de los subprocesos y relaciones que lo componen. De esta manera se garantiza el óptimo funcionamiento requerido para proveer y mantener los servicios y soluciones integrales de tecnologías de información.

Published
2022
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4. Cardiomyocyte transcriptomic signatures in response toTrypanosoma cruziinfection underpin Chagas cardiomyopathy progression

Author

Katherine-Sofia Candray-Medina, Yu Nakagama, Masamichi Ito, Shun Nakagama, Evariste Tshibangu-Kabamba, Norihiko Takeda, Yuki Sugiura, Yuko Nitahara, Yu Michimuko-Nagahara, Natsuko Kaku, Yoko Onizuka, Carmen-Elena Arias, Maricela Mejia, Karla Alas, Susana Peña, Yasuhiro Maejima, Issei Komuro, Junko Nakajima-Shimada, and Yasutoshi Kido

Abstract

Chagas disease can lead to life-threatening cardiac manifestations that occur more frequently in geographic areas more prevalent with the TcI/II circulating genetic strains. To elucidate the differential transcriptomic signatures of the cardiomyocyte resulting from infection with TcI/II or TcVIT. cruzistrains and explore their relationships with pathogenesis, HL-1 rodent cardiomyocytes were infected with TcI/II or TcVIT. cruzitrypomastigotes. RNA was isolated serially post-infection for microarray analysis. Enrichment analyses of differentially expressed genes (fold-change ≥2 or ≤ 0.5) highlighted the over-represented biological pathways. We found that Oxidative stress-related GO terms, ‘Hypertrophy model’, ‘Apoptosis’, and ‘MAPK signaling’ pathways (all with pImportanceChagas disease affects more than 6 million people worldwide. One-third of those chronically infected will develop the life-threatening condition Chagas Cardiomyopathy (CCM).Trypanosoma cruzi (T. cruzi), grouped based on their genetic variability into six discrete typing units (DTU), are associated with DTU-specific clinical phenotypes. The diverse genetic make-up of parasite virulence factors shall evoke unique host defense responses of variable magnitude, collectively affecting the phenotypic expression of CCM. To address this, we performed a transcriptome analysis of cardiomyocytes infected with three differentT. cruzistrains each belonging to a different DTU. As a result, we were able to point out dysregulation in nitrogen metabolic processes, Glutathione, and one-carbon metabolism pathways as main features in the host response against cardiomyopathy-proneT. cruzistrains. Further research on these pathways could serve not only in the lookout for progression biomarkers but also in the lead toward the discovery of new therapeutic targets.

Published
2023
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5. Optimization of Antitrypanosomatid Agents: Identification of Nonmutagenic Drug Candidates with in Vivo Activity

Author

Pablo Márquez, Sandra Milena Leal, Hugo Cerecetto, Martín Gabay, Marlus Chorilli, Guzmán Álvarez, Patricia Escobar, Oscar E. Piro, Javier Varela, Mercedes González, Elva Serna, Susana Torres, Gloria Yaluff, Ninfa Vera de Bilbao, Karina Cuchilla, Gustavo A. Echeverría, and Carmen Elena Arias Rivas

Subjects
Drug, Chagas disease, Trypanosoma cruzi, Ciencias Físicas, media_common.quotation_subject, Pharmacology, Otras Ciencias Físicas, Mice, Drug Stability, Mutagenicity, In vivo, parasitic diseases, Drug Discovery, medicine, Animals, media_common, Mice, Inbred BALB C, biology, Chemistry, biology.organism_classification, medicine.disease, Trypanocidal Agents, In vitro, Mutation, Toxicity, Molecular Medicine, Female, In vivo-activity, Identification (biology), Thiazole, CIENCIAS NATURALES Y EXACTAS
Abstract

Chagas disease, caused by Trypanosoma cruzi parasite, was described thousands of years ago. Currently, it affects millions of people, mostly in Latin America, and there are not suitable drugs for treating it. As an attempt to find appropriate drugs to deal with this problem, we report here on the design, synthesis and characterization of eighty-two new compounds. Trypanosomicidal behavior in vitro showed more than twenty outstanding derivatives with anti-Trypanosoma cruzi activity. Furthermore, we studied the nonspecific toxicity against mammalian cells determining their selectivity and also performed mutagenicity studies. Proof of concept, in vivo studies, was conducted with two of the most promising derivatives (77 and 80). They were identified as candidates because they have: (i) very simple and cost-effective syntheses; (ii) activity against different stages and strains of the parasite showing excellent in vivo behavior during the acute phase of Chagas disease; (iii) neither nonspecific toxicity nor mutagenic activity. Fil: Guzmán Álvarez, Javier Varela. Universidad de la República; Uruguay Fil: Varela, Javier. Universidad de la República; Uruguay Fil: Márquez, Pablo. Universidad de la República; Uruguay Fil: Gabay, Martín. Universidad de la República; Uruguay Fil: Arias Rivas, Carmen Elena. Centro Nacional de Investigaciones Científicas; El Salvador Fil: Cuchilla, Karina. Centro Nacional de Investigaciones Científicas; El Salvador Fil: Echeverría, Gustavo Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física La Plata; Argentina Fil: Piro, Oscar Enrique. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física La Plata; Argentina Fil: Chorilli, Marlus. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil Fil: Leal, Sandra M.. Universidad Industrial Santander; Colombia Fil: Escobar, Patricia. Universidad Industrial Santander; Colombia Fil: Serna, Elva. Universidad Nacional de Asunción; Paraguay Fil: Torres, Susana. Universidad Nacional de Asunción; Paraguay Fil: Yaluff, Gloria. Universidad Nacional de Asunción; Paraguay Fil: Vera de Bilbao, Ninfa I.. Universidad Nacional de Asunción; Paraguay Fil: González, Mercedes. Universidad de la República; Uruguay Fil: Cerecetto, Hugo. Universidad de la República; Uruguay

Published
2014
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