1. Pooling of urine samples for molecular detection of Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium as a screening strategy among young adults in Catalonia
- Author
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Evelin López-Corbeto, Victoria González, Rossie Lugo, Belen Rivaya, Jordi Casabona, Lurdes Matas, Nayade Crespo, M. Jose Garrofe, Rosa Alzuria, Elena Bureu, Isabel Fernandez, Montserrat Piquet, Yolanda Florensa, Carme Sarroca, Judit Mari, Elisabet Mitjans, Ivana Jorda, Sonia Garcia, Hildegard Mausbach, Teresa Gomez, Ainhoa Borras, Alba Llobera, Ana Estruch, Mabel Cayuela, Josep Grau, Judit Tarres, Dolors Guix, Lorena Serrano, Paula Amezcua, Meritxell Angelet, Carmen Barrionuevo, Rocio Calviño, Margarida Colldeforns, Mercè Duran, M. Jose Garcia, Maria Gonzalez, Gemma Hernandez, Jon Ander Modenes, Anna Vila, Carme Basset, Angels Avecilla, M. Pilar Blasco, Lucia Burgos, Ramon Espelt, Edith Lopez-Grado, M. Ines Molina, Elisenda Prats, Demetria Patricio, Gemma March, Irene Aguilar, Sonia Argiles, M. Jose Bayarri, Judith Bertran, Carmen Burgos, Montserrat Carreres, Ana Corredor, Victoria Fernandez, Gemma Martinez, Gracia Moreno, Alba Pallie, Teresa Pinto, Maria Ramirez, Cristina Rebollo, Eusebia Romano, M. Consuelo Muxi, Rosa Escriche, Judit Pelegri, Luciano Carmelo Caccioppoli, Elisabet Grau, Jordi Xandri, M. Julia Cid, Laia Aguilar, Helena Almenar, Carlos Navales, Jordi Baroja, M. Paz Oliver, Carme Fornells, Marta Hernandez, and Estrella Arranz
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0301 basic medicine ,Microbiology (medical) ,medicine.medical_specialty ,Adolescent ,030106 microbiology ,Pooling ,Population ,Sexually Transmitted Diseases ,Context (language use) ,Chlamydia trachomatis ,Mycoplasma genitalium ,Urine ,urologic and male genital diseases ,medicine.disease_cause ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Environmental health ,Epidemiology ,medicine ,Humans ,030212 general & internal medicine ,education ,Reproductive health ,education.field_of_study ,biology ,business.industry ,biology.organism_classification ,female genital diseases and pregnancy complications ,Neisseria gonorrhoeae ,Cross-Sectional Studies ,Spain ,business - Abstract
Introduction Bacterial sexually transmitted infections (STIs) have an important impact on reproductive health, highlighting the increase in Chlamydia trachomatis infection rates among young people. To reduce the costs of STI detection, the pooling strategy is beneficial for high-throughput tests in low-prevalence populations using non-invasive samples. Objectives (1) To describe the performance of a 7-STI PCR assay using the pooling of three urine samples to detect C. trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium; (2) to estimate the cost saving of the pooling strategy; (3) to describe the prevalence, risk factors and coinfections of C. trachomatis, N. gonorrhoeae and M. genitalium in young people ≤25 years in Catalonia. Methods cross-sectional prevalence study conducted in 2016 among young people ≤25 years of age seen in sexual and reproductive health centres throughout Catalonia from pools of three urine samples. A standardized questionnaire was used to collect clinical-epidemiological and behavioural variables. Results 1032 young people were tested. The prevalence of C. trachomatis, N. gonorrhoeae and M. genitalium was 8.5%, 0.6% and 3.5%, respectively. The pooling strategy provided a 33% savings in reagent costs. Conclusions The pooling strategy implemented for epidemiological studies in our context provides a savings that has an impact on the viability of STI detection programmes. In the same way, this study shows that C. trachomatis prevalence continues to increase in this population and, for the first time in Catalonia, the prevalence of M. genitalium in young people is shown.
- Published
- 2019