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46 results on '"Carlotti Jr, Carlos Gilberto"'

1. Clinical Prognostic Implications of Wnt Hub Genes Expression in Medulloblastoma

Author

Martins-da-Silva, Andrea, Baroni, Mirella, Salomão, Karina Bezerra, das Chagas, Pablo Ferreira, Bonfim-Silva, Ricardo, Geron, Lenisa, Cruzeiro, Gustavo Alencastro Veiga, da Silva, Jr, Wilson Araújo, Corrêa, Carolina Alves Pereira, Carlotti, Jr, Carlos Gilberto, de Paula Queiroz, Rosane Gomes, Marie, Suely Kazue Nagahashi, Brandalise, Silvia Regina, Yunes, José Andrés, Scrideli, Carlos Alberto, Valera, Elvis Terci, and Tone, Luiz Gonzaga

Published
2023
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3. High-throughput microRNA profile in adult and pediatric primary glioblastomas: the role of miR-10b-5p and miR-630 in the tumor aggressiveness

Author

Junior, Luiz Guilherme Darrigo, Baroni, Mirella, Lira, Régia Caroline Peixoto, Teixeira, Silvia, Fedatto, Paola Fernanda, Silveira, Vanessa Silva, Suazo, Veridiana Kill, Veronez, Luciana Chain, Panepucci, Rodrigo Alexandre, Antônio, David Santos Marco, Yunes, José Andres, Brandalise, Silvia Regina, dos Santos Aguiar, Simone, Neder, Luciano, de Oliveira, Ricardo Santos, Machado, Hélio Rubens, Carlotti, Jr, Carlos Gilberto, Tone, Luiz Gonzaga, Valera, Elvis Terci, and Scrideli, Carlos Alberto

Published
2020
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4. Expression of MicroRNAs miR-145, miR-181c, miR-199a and miR-1183 in the Blood and Hippocampus of Patients with Mesial Temporal Lobe Epilepsy

Author

Antônio, Luana Grupioni Lourenço, Freitas-Lima, Priscila, Pereira-da-Silva, Gabriela, Assirati, Jr, João Alberto, Matias, Caio Marconato, Cirino, Mucio Luiz Assis, Tirapelli, Luis Fernando, Velasco, Tonicarlo Rodrigues, Sakamoto, Americo Ceiki, Carlotti, Jr, Carlos Gilberto, and Tirapelli, Daniela Pretti da Cunha

Published
2019
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6. Expression of microRNAs miR-21 and miR-326 associated with HIF-1α regulation in neurospheres of glioblastoma submitted to ionizing radiation treatment

Author

Turra, Letícia Passi, primary, Rodrigues, Andressa Romualdo, additional, Lizarte Neto, Fermino Sanches, additional, Novais, Paulo Cezar, additional, Nunes, Maria Julia, additional, Tirapelli, Victor Cunha, additional, Peria, Fernanda Maris, additional, Carneiro, Vinícius Marques, additional, Cirino, Mucio Luiz De Assis, additional, Carlotti Jr, Carlos Gilberto, additional, and Tirapelli, Daniela Pretti da Cunha, additional

Published
2022
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7. Online graduate activities during the pandemic at the University of São Paulo, Brazil.

Author

Nunes, Fátima L. S., Trindade-Suedam, Ivy Kiemle, Carlotti Jr, Carlos Gilberto, Marangoni, Yara R., Fischer Rubira-Bullen, Izabel Regina, Barreto-Chaves, Maria Luiza, and Gir, Elucir

Subjects
VIRTUAL classrooms, DISTANCE education, ONLINE education, SARS-CoV-2, PANDEMICS
Abstract

The pandemic caused by SARS-COV-2 virus required changes in the academic procedures and routines in general. In March 2020, the University of São Paulo (USP) decided to temporarily replace face-to-face classes by remote online learning using digital platforms. Due to its considerable number of faculties (approximately 6000) and graduate students (approximately 30,000) from the 267 Graduate Programs, USP had to face the challenge of dealing with new forms of learning, as well as new ways to make feasible thesis and dissertation defenses. In order to evaluate the changes required during the pandemic, the University Provost for graduate affairs conducted a survey, by using forms containing questions in order to allow the academic community the identification of the best and most efficient practices used by faculty members, as well as weaknesses that should be overcome. This article presents and discusses the results of this evaluation, considering 1454 answers about defenses and 2656 answers about online courses. The results showed that most of the faculty members and students approved the procedures adopted, but some improvements regarding remote defense sessions and learning strategies were necessary. The article also presents some immediate actions performed by USP in order to overcome the challenges presented during remote activities. [ABSTRACT FROM AUTHOR]

Published
2023
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10. Tetra-O-methyl nordihydroguaiaretic acid, an inhibitor of Sp1-mediated survivin transcription, induces apoptosis and acts synergistically with chemo-radiotherapy in glioblastoma cells

Author

Castro-Gamero, Angel Mauricio, Borges, Kleiton Silva, Moreno, Daniel Antunes, Suazo, Veridiana Kill, Fujinami, Mayara Missono, de Paula Gomes Queiroz, Rosane, de Oliveira, Harley Francisco, Carlotti, Jr., Carlos Gilberto, Scrideli, Carlos Alberto, and Tone, Luiz Gonzaga

Published
2013
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15. AIF, PARP-1 and MicroRNA-9 Expression in Cerebral Ischemia Associated to Alcoholism Experimental Model

Author

Abrahão, Dayana Pousa Siqueira, Lizarte Neto, Fermino Sanches, Rodrigues, Andressa Romualdo, Cirino, Múcio Luiz de Assis, Silva, Jairo Pinheiro da, Sarraipo, Vagner Schiavoni, Carvalho, Camila Albuquerque Melo de, Tazima, Maria de Fátima Galli Sorita, Carlotti-Jr., Carlos Gilberto, Colli, Benedicto Oscar, Tirapelli, Luís Fernando, and Tirapelli, Daniela Pretti da Cunha

Subjects
AIF, Alcoholism, miRNA-9, Alcoholismo, microRNA-9, Isquemia cerebral, PARP-1, Cerebral ischemia
Abstract

SUMMARY: The chronic consumption of alcohol causes a worsening of the events that follow the cerebral ischemia. These events are regulated through the expression of several genes and microRNAs. The aimof this work was To analyze and describe the expression profile of PARP and AIF and miRNA-9 proteins in rats submitted to focal cerebral ischemia, associated or not with chronic alcoholism model. Methods: Twenty adult Wistar rats, subdivided into: control; ischemic; alcoholic and ischemic / alcoholized for immunohistochemical analysis and miRNA-9 gene expression. Results: There was a reduction in the protein expression of PARP-1 and a positive marking for AIF in the ischemic / alcoholized group. The miRNA-9 did not obtain significant expression. The association of ischemia with chronic alcohol use promoted a tendency to low expression of miRNA-9, low expression of PARP-1 and high expression of AIF, indicating an interference in the protective effect of miRNA-9 be observed in the other groups. RESUMEN: El consumo crónico de alcohol provoca un empeoramiento de los eventos que siguen a la isquemia cerebral. Estos eventos están regulados a través de la expresión de varios genes y microRNA. El objetivo de este trabajo fue analizar y describir el perfil de expresión de las proteínas PARP y AIF y microRNA-9 en ratas sometidas a isquemia cerebral focal, asociadas o no, con el modelo de alcoholismo crónico. Veinte ratas Wistar adultas se dividieron en: grupo control, isquémico alcohólico, e isquémico / alcoholizado para análisis inmunohistoquímico y expresión de genes microRNA-9. Resultados: Hubo una reducción en la expresión de proteínas de PARP-1 y un marcado positivo para AIF en el grupo isquémico / alcoholizado. No se observó una expresión significativa en el microRNA-9. La asociación de la isquemia con el consumo crónico de alcohol promovió una tendencia a la baja expresión de microRNA-9, baja expresión de PARP1 y alta expresión de AIF, lo que indica una interferencia en el efecto protector de microRNA-9 en los otros grupos.

Published
2020

16. Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen

Author

Tirapelli, Daniela P. C, Cirino, Mucio Luiz de Assis, Carvalho, Camila A. Melo de, Novais, Paulo Cezar, Figueredo, Rafael Zimak, Porsani, Lucas Barbosa, Gula, Isabella Stracieri, Rodrigues, Andressa Romualdo, Silva, Jairo Pinheiro da, Pereira, Adriana Lis, Lizarte Neto, Fermino Sanches, Schimming, Bruno Cesar, Tazima, Maria de Fátima Galli Sorita, Fazan, Valéria de Paula Sassoli, Carlotti-Jr, Carlos Gilberto, Tirapelli, Luis Fernando, and Colli, Benedicto Oscar

Subjects
Caspase-3, Ketoprofen, Neuroprotección, Caspasa-3, Isquemia cerebral, Apoptosis, Gene expression, Hypothermia, Cerebral ischemia, Ketoprofeno, Neuroprotection, Hipotermia, Expresión génica
Abstract

SUMMARY: This study aimed to investigate the morphometric and the pattern of protein and gene expression related to the extrinsic apoptotic pathway in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. For this analysis, 120 rats were randomly divided into 3 groups (20 animals each): control - no surgery (20 animals); sham - simulation of surgery (20 animals); ischemic - focal ischemia for 1 hour, without reperfusion (80 animals) and divided into four subgroups with 20 animals each: ischemic + intraischemic hypothermia; ischemic + previous intravenous ketoprofen, and ischemic + hypothermia and ketoprofen. The infarct volume was measured using morphometric analysis of infarct areas defined by triphenyl tetrazolium chloride and the patterns of expression of the apoptosis genes (Fas, c-Flip, caspase-8 and caspase-3) and the apoptosis protein caspase-3 were evaluated by quantitative real-time PCR and immunohistochemistry, respectively. Hypo expression of genes of extrinsic pathway of apoptosis was observed: Fas receptor, c-Flip and caspase-8 in the ischemics areas. Increases in the gene and protein caspase-3 in the ischemic areas were also observed, and these increases were reduced by hypothermia and ketoprofen, also noted in the morphometric study. The caspases-3 increase suggests that this gene plays an important role in apoptosis, probably culminating in cell death and that the neuroprotective effect of hypothermia and ketoprofen is involved. RESUMEN: Este estudio tuvo como objetivo investigar la morfometría y el patrón de expresión de proteínas y genes relacionados con la vía apoptótica extrínseca en la isquemia cerebral focal experimental y el agujero de neuroprotección con hipotermia y ketoprofeno. Se dividieron aleatoriamente 120 ratas en 3 grupos (20 animales cada uno): control - sin cirugía (20 animales); simulación - simulación de cirugía (20 animales); isquemia isquemia focal durante 1 hora, sin reperfusión (80 animales) y dividida en cuatro subgrupos con 20 animales cada uno: isquemia + hipotermia intraisquémica; isquemia + ketoprofeno intravenoso previo, e isquemia + hipotermia y ketoprofeno. El volumen del infarto se midió utilizando un análisis morfométrico de áreas de infarto definidas por cloruro de trifenil tetrazolio y los patrones de expresión de los genes de apoptosis (Fas, c-Flip, caspase-8 y caspase-3) y la proteína de apoptosis caspase-3 fueron evaluados por PCR cuantitativa en tiempo real e inmunohistoquímica, respectivamente. Se observó hipoexpresión de genes de la vía extrínseca de la apoptosis: receptor Fas, c-Flip y caspasa-8 en las áreas isquémicas. También se observaron aumentos en el gen y la proteína caspasa-3 en las áreas isquémicas y estos aumentos se redujeron por hipotermia y ketoprofeno, también observado por estudio morfométrico. El aumento de caspasas-3 sugiere que este gen tiene un papel importante en la apoptosis, y probable causa de muerte celular, involucrando el efecto neuroprotector de la hipotermia y el ketoprofeno.

Published
2020

17. AIF, PARP-1 and MicroRNA-9 Expression in Cerebral Ischemia Associated to Alcoholism Experimental Model

Author

Abrahão, Dayana Pousa Siqueira, primary, Lizarte Neto, Fermino Sanches, additional, Rodrigues, Andressa Romualdo, additional, Cirino, Múcio Luiz de Assis, additional, Silva, Jairo Pinheiro da, additional, Sarraipo, Vagner Schiavoni, additional, Carvalho, Camila Albuquerque Melo de, additional, Tazima, Maria de Fátima Galli Sorita, additional, Carlotti-Jr., Carlos Gilberto, additional, Colli, Benedicto Oscar, additional, Tirapelli, Luís Fernando, additional, and Tirapelli, Daniela Pretti da Cunha, additional

Published
2020
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18. Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen

Author

Tirapelli, Daniela P. C, primary, Cirino, Mucio Luiz de Assis, additional, Carvalho, Camila A. Melo de, additional, Novais, Paulo Cezar, additional, Figueredo, Rafael Zimak, additional, Porsani, Lucas Barbosa, additional, Gula, Isabella Stracieri, additional, Rodrigues, Andressa Romualdo, additional, Silva, Jairo Pinheiro da, additional, Pereira, Adriana Lis, additional, Lizarte Neto, Fermino Sanches, additional, Schimming, Bruno Cesar, additional, Tazima, Maria de Fátima Galli Sorita, additional, Fazan, Valéria de Paula Sassoli, additional, Carlotti-Jr, Carlos Gilberto, additional, Tirapelli, Luis Fernando, additional, and Colli, Benedicto Oscar, additional

Published
2020
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20. Analysis of Caspase-9 protein and microRNAs miR-21, miR-126 and miR-155 related to the apoptosis mechanism in the cerebellum of rats submitted to focal cerebral ischemia associated with an alcoholism model

Author

SILVA, Jairo Pinheiro da, primary, LIZARTE NETO, Fermino Sanches, additional, CIRINO, Mucio Luiz de Assis, additional, CARVALHO, Camila Albuquerque Melo de, additional, CARLOTTI JR, Carlos Gilberto, additional, COLLI, Benedicto Oscar, additional, TIRAPELLI, Daniela Pretti da Cunha, additional, and TIRAPELLI, Luís Fernando, additional

Published
2019
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21. microRNA-181d associated with the methylation status of the MGMT gene in Glioblastoma multiforme cancer stem cells submitted to treatments with ionizing radiation and temozolomide

Author

Lizarte Neto, Fermino Sanches, primary, Rodrigues, Andressa Romualdo, additional, Trevisan, Felipe Amstalden, additional, de Assis Cirino, Mucio Luiz, additional, Matias, Caio César Marconato Simões, additional, Pereira-da-Silva, Gabriela, additional, Peria, Fernanda Maris, additional, Tirapelli, Daniela Pretti da Cunha, additional, and Carlotti Jr., Carlos Gilberto, additional

Published
2019
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22. Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism

Author

Schiavoni, Vagner Sarraipo, primary, Silva, Jairo Pinheiro da, additional, Lizarte Neto, Fermino Sanches, additional, Assis, Múcio Luiz Cirino de, additional, Tazima, Maria de Fátima Galli Sorita, additional, Carvalho, Camila Albuquerque Melo de, additional, Tirapelli, Daniela Pretti da Cunha, additional, Carlotti Jr, Carlos Gilberto, additional, Colli, Benedicto Oscar, additional, and Tirapelli, Luis Fernando, additional

Published
2018
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23. An Integrated TCGA Pan-Cancer Clinical Data Resource to Drive High-Quality Survival Outcome Analytics

Author

Liu, Jianfang, primary, Lichtenberg, Tara, additional, Hoadley, Katherine A., additional, Poisson, Laila M., additional, Lazar, Alexander J., additional, Cherniack, Andrew D., additional, Kovatich, Albert J., additional, Benz, Christopher C., additional, Levine, Douglas A., additional, Lee, Adrian V., additional, Omberg, Larsson, additional, Wolf, Denise M., additional, Shriver, Craig D., additional, Thorsson, Vesteinn, additional, Hu, Hai, additional, Caesar-Johnson, Samantha J., additional, Demchok, John A., additional, Felau, Ina, additional, Kasapi, Melpomeni, additional, Ferguson, Martin L., additional, Hutter, Carolyn M., additional, Sofia, Heidi J., additional, Tarnuzzer, Roy, additional, Wang, Zhining, additional, Yang, Liming, additional, Zenklusen, Jean C., additional, Zhang, Jiashan (Julia), additional, Chudamani, Sudha, additional, Liu, Jia, additional, Lolla, Laxmi, additional, Naresh, Rashi, additional, Pihl, Todd, additional, Sun, Qiang, additional, Wan, Yunhu, additional, Wu, Ye, additional, Cho, Juok, additional, DeFreitas, Timothy, additional, Frazer, Scott, additional, Gehlenborg, Nils, additional, Getz, Gad, additional, Heiman, David I., additional, Kim, Jaegil, additional, Lawrence, Michael S., additional, Lin, Pei, additional, Meier, Sam, additional, Noble, Michael S., additional, Saksena, Gordon, additional, Voet, Doug, additional, Zhang, Hailei, additional, Bernard, Brady, additional, Chambwe, Nyasha, additional, Dhankani, Varsha, additional, Knijnenburg, Theo, additional, Kramer, Roger, additional, Leinonen, Kalle, additional, Liu, Yuexin, additional, Miller, Michael, additional, Reynolds, Sheila, additional, Shmulevich, Ilya, additional, Zhang, Wei, additional, Akbani, Rehan, additional, Broom, Bradley M., additional, Hegde, Apurva M., additional, Ju, Zhenlin, additional, Kanchi, Rupa S., additional, Korkut, Anil, additional, Li, Jun, additional, Liang, Han, additional, Ling, Shiyun, additional, Liu, Wenbin, additional, Lu, Yiling, additional, Mills, Gordon B., additional, Ng, Kwok-Shing, additional, Rao, Arvind, additional, Ryan, Michael, additional, Wang, Jing, additional, Weinstein, John N., additional, Zhang, Jiexin, additional, Abeshouse, Adam, additional, Armenia, Joshua, additional, Chakravarty, Debyani, additional, Chatila, Walid K., additional, de Bruijn, Ino, additional, Gao, Jianjiong, additional, Gross, Benjamin E., additional, Heins, Zachary J., additional, Kundra, Ritika, additional, La, Konnor, additional, Ladanyi, Marc, additional, Luna, Augustin, additional, Nissan, Moriah G., additional, Ochoa, Angelica, additional, Phillips, Sarah M., additional, Reznik, Ed, additional, Sanchez-Vega, Francisco, additional, Sander, Chris, additional, Schultz, Nikolaus, additional, Sheridan, Robert, additional, Sumer, S. Onur, additional, Sun, Yichao, additional, Taylor, Barry S., additional, Wang, Jioajiao, additional, Zhang, Hongxin, additional, Anur, Pavana, additional, Peto, Myron, additional, Spellman, Paul, additional, Benz, Christopher, additional, Stuart, Joshua M., additional, Wong, Christopher K., additional, Yau, Christina, additional, Hayes, D. Neil, additional, Parker, Joel S., additional, Wilkerson, Matthew D., additional, Ally, Adrian, additional, Balasundaram, Miruna, additional, Bowlby, Reanne, additional, Brooks, Denise, additional, Carlsen, Rebecca, additional, Chuah, Eric, additional, Dhalla, Noreen, additional, Holt, Robert, additional, Jones, Steven J.M., additional, Kasaian, Katayoon, additional, Lee, Darlene, additional, Ma, Yussanne, additional, Marra, Marco A., additional, Mayo, Michael, additional, Moore, Richard A., additional, Mungall, Andrew J., additional, Mungall, Karen, additional, Robertson, A. Gordon, additional, Sadeghi, Sara, additional, Schein, Jacqueline E., additional, Sipahimalani, Payal, additional, Tam, Angela, additional, Thiessen, Nina, additional, Tse, Kane, additional, Wong, Tina, additional, Berger, Ashton C., additional, Beroukhim, Rameen, additional, Cibulskis, Carrie, additional, Gabriel, Stacey B., additional, Gao, Galen F., additional, Ha, Gavin, additional, Meyerson, Matthew, additional, Schumacher, Steven E., additional, Shih, Juliann, additional, Kucherlapati, Melanie H., additional, Kucherlapati, Raju S., additional, Baylin, Stephen, additional, Cope, Leslie, additional, Danilova, Ludmila, additional, Bootwalla, Moiz S., additional, Lai, Phillip H., additional, Maglinte, Dennis T., additional, Van Den Berg, David J., additional, Weisenberger, Daniel J., additional, Auman, J. Todd, additional, Balu, Saianand, additional, Bodenheimer, Tom, additional, Fan, Cheng, additional, Hoyle, Alan P., additional, Jefferys, Stuart R., additional, Jones, Corbin D., additional, Meng, Shaowu, additional, Mieczkowski, Piotr A., additional, Mose, Lisle E., additional, Perou, Amy H., additional, Perou, Charles M., additional, Roach, Jeffrey, additional, Shi, Yan, additional, Simons, Janae V., additional, Skelly, Tara, additional, Soloway, Matthew G., additional, Tan, Donghui, additional, Veluvolu, Umadevi, additional, Fan, Huihui, additional, Hinoue, Toshinori, additional, Laird, Peter W., additional, Shen, Hui, additional, Zhou, Wanding, additional, Bellair, Michelle, additional, Chang, Kyle, additional, Covington, Kyle, additional, Creighton, Chad J., additional, Dinh, Huyen, additional, Doddapaneni, HarshaVardhan, additional, Donehower, Lawrence A., additional, Drummond, Jennifer, additional, Gibbs, Richard A., additional, Glenn, Robert, additional, Hale, Walker, additional, Han, Yi, additional, Hu, Jianhong, additional, Korchina, Viktoriya, additional, Lee, Sandra, additional, Lewis, Lora, additional, Li, Wei, additional, Liu, Xiuping, additional, Morgan, Margaret, additional, Morton, Donna, additional, Muzny, Donna, additional, Santibanez, Jireh, additional, Sheth, Margi, additional, Shinbro, Eve, additional, Wang, Linghua, additional, Wang, Min, additional, Wheeler, David A., additional, Xi, Liu, additional, Zhao, Fengmei, additional, Hess, Julian, additional, Appelbaum, Elizabeth L., additional, Bailey, Matthew, additional, Cordes, Matthew G., additional, Ding, Li, additional, Fronick, Catrina C., additional, Fulton, Lucinda A., additional, Fulton, Robert S., additional, Kandoth, Cyriac, additional, Mardis, Elaine R., additional, McLellan, Michael D., additional, Miller, Christopher A., additional, Schmidt, Heather K., additional, Wilson, Richard K., additional, Crain, Daniel, additional, Curley, Erin, additional, Gardner, Johanna, additional, Lau, Kevin, additional, Mallery, David, additional, Morris, Scott, additional, Paulauskis, Joseph, additional, Penny, Robert, additional, Shelton, Candace, additional, Shelton, Troy, additional, Sherman, Mark, additional, Thompson, Eric, additional, Yena, Peggy, additional, Bowen, Jay, additional, Gastier-Foster, Julie M., additional, Gerken, Mark, additional, Leraas, Kristen M., additional, Lichtenberg, Tara M., additional, Ramirez, Nilsa C., additional, Wise, Lisa, additional, Zmuda, Erik, additional, Corcoran, Niall, additional, Costello, Tony, additional, Hovens, Christopher, additional, Carvalho, Andre L., additional, de Carvalho, Ana C., additional, Fregnani, José H., additional, Longatto-Filho, Adhemar, additional, Reis, Rui M., additional, Scapulatempo-Neto, Cristovam, additional, Silveira, Henrique C.S., additional, Vidal, Daniel O., additional, Burnette, Andrew, additional, Eschbacher, Jennifer, additional, Hermes, Beth, additional, Noss, Ardene, additional, Singh, Rosy, additional, Anderson, Matthew L., additional, Castro, Patricia D., additional, Ittmann, Michael, additional, Huntsman, David, additional, Kohl, Bernard, additional, Le, Xuan, additional, Thorp, Richard, additional, Andry, Chris, additional, Duffy, Elizabeth R., additional, Lyadov, Vladimir, additional, Paklina, Oxana, additional, Setdikova, Galiya, additional, Shabunin, Alexey, additional, Tavobilov, Mikhail, additional, McPherson, Christopher, additional, Warnick, Ronald, additional, Berkowitz, Ross, additional, Cramer, Daniel, additional, Feltmate, Colleen, additional, Horowitz, Neil, additional, Kibel, Adam, additional, Muto, Michael, additional, Raut, Chandrajit P., additional, Malykh, Andrei, additional, Barnholtz-Sloan, Jill S., additional, Barrett, Wendi, additional, Devine, Karen, additional, Fulop, Jordonna, additional, Ostrom, Quinn T., additional, Shimmel, Kristen, additional, Wolinsky, Yingli, additional, Sloan, Andrew E., additional, De Rose, Agostino, additional, Giuliante, Felice, additional, Goodman, Marc, additional, Karlan, Beth Y., additional, Hagedorn, Curt H., additional, Eckman, John, additional, Harr, Jodi, additional, Myers, Jerome, additional, Tucker, Kelinda, additional, Zach, Leigh Anne, additional, Deyarmin, Brenda, additional, Kvecher, Leonid, additional, Larson, Caroline, additional, Mural, Richard J., additional, Somiari, Stella, additional, Vicha, Ales, additional, Zelinka, Tomas, additional, Bennett, Joseph, additional, Iacocca, Mary, additional, Rabeno, Brenda, additional, Swanson, Patricia, additional, Latour, Mathieu, additional, Lacombe, Louis, additional, Têtu, Bernard, additional, Bergeron, Alain, additional, McGraw, Mary, additional, Staugaitis, Susan M., additional, Chabot, John, additional, Hibshoosh, Hanina, additional, Sepulveda, Antonia, additional, Su, Tao, additional, Wang, Timothy, additional, Potapova, Olga, additional, Voronina, Olga, additional, Desjardins, Laurence, additional, Mariani, Odette, additional, Roman-Roman, Sergio, additional, Sastre, Xavier, additional, Stern, Marc-Henri, additional, Cheng, Feixiong, additional, Signoretti, Sabina, additional, Berchuck, Andrew, additional, Bigner, Darell, additional, Lipp, Eric, additional, Marks, Jeffrey, additional, McCall, Shannon, additional, McLendon, Roger, additional, Secord, Angeles, additional, Sharp, Alexis, additional, Behera, Madhusmita, additional, Brat, Daniel J., additional, Chen, Amy, additional, Delman, Keith, additional, Force, Seth, additional, Khuri, Fadlo, additional, Magliocca, Kelly, additional, Maithel, Shishir, additional, Olson, Jeffrey J., additional, Owonikoko, Taofeek, additional, Pickens, Alan, additional, Ramalingam, Suresh, additional, Shin, Dong M., additional, Sica, Gabriel, additional, Van Meir, Erwin G., additional, Zhang, Hongzheng, additional, Eijckenboom, Wil, additional, Gillis, Ad, additional, Korpershoek, Esther, additional, Looijenga, Leendert, additional, Oosterhuis, Wolter, additional, Stoop, Hans, additional, van Kessel, Kim E., additional, Zwarthoff, Ellen C., additional, Calatozzolo, Chiara, additional, Cuppini, Lucia, additional, Cuzzubbo, Stefania, additional, DiMeco, Francesco, additional, Finocchiaro, Gaetano, additional, Mattei, Luca, additional, Perin, Alessandro, additional, Pollo, Bianca, additional, Chen, Chu, additional, Houck, John, additional, Lohavanichbutr, Pawadee, additional, Hartmann, Arndt, additional, Stoehr, Christine, additional, Stoehr, Robert, additional, Taubert, Helge, additional, Wach, Sven, additional, Wullich, Bernd, additional, Kycler, Witold, additional, Murawa, Dawid, additional, Wiznerowicz, Maciej, additional, Chung, Ki, additional, Edenfield, W. Jeffrey, additional, Martin, Julie, additional, Baudin, Eric, additional, Bubley, Glenn, additional, Bueno, Raphael, additional, De Rienzo, Assunta, additional, Richards, William G., additional, Kalkanis, Steven, additional, Mikkelsen, Tom, additional, Noushmehr, Houtan, additional, Scarpace, Lisa, additional, Girard, Nicolas, additional, Aymerich, Marta, additional, Campo, Elias, additional, Giné, Eva, additional, Guillermo, Armando López, additional, Van Bang, Nguyen, additional, Hanh, Phan Thi, additional, Phu, Bui Duc, additional, Tang, Yufang, additional, Colman, Howard, additional, Evason, Kimberley, additional, Dottino, Peter R., additional, Martignetti, John A., additional, Gabra, Hani, additional, Juhl, Hartmut, additional, Akeredolu, Teniola, additional, Stepa, Serghei, additional, Hoon, Dave, additional, Ahn, Keunsoo, additional, Kang, Koo Jeong, additional, Beuschlein, Felix, additional, Breggia, Anne, additional, Birrer, Michael, additional, Bell, Debra, additional, Borad, Mitesh, additional, Bryce, Alan H., additional, Castle, Erik, additional, Chandan, Vishal, additional, Cheville, John, additional, Copland, John A., additional, Farnell, Michael, additional, Flotte, Thomas, additional, Giama, Nasra, additional, Ho, Thai, additional, Kendrick, Michael, additional, Kocher, Jean-Pierre, additional, Kopp, Karla, additional, Moser, Catherine, additional, Nagorney, David, additional, O’Brien, Daniel, additional, O’Neill, Brian Patrick, additional, Patel, Tushar, additional, Petersen, Gloria, additional, Que, Florencia, additional, Rivera, Michael, additional, Roberts, Lewis, additional, Smallridge, Robert, additional, Smyrk, Thomas, additional, Stanton, Melissa, additional, Thompson, R. Houston, additional, Torbenson, Michael, additional, Yang, Ju Dong, additional, Zhang, Lizhi, additional, Brimo, Fadi, additional, Ajani, Jaffer A., additional, Angulo Gonzalez, Ana Maria, additional, Behrens, Carmen, additional, Bondaruk, Jolanta, additional, Broaddus, Russell, additional, Czerniak, Bogdan, additional, Esmaeli, Bita, additional, Fujimoto, Junya, additional, Gershenwald, Jeffrey, additional, Guo, Charles, additional, Logothetis, Christopher, additional, Meric-Bernstam, Funda, additional, Moran, Cesar, additional, Ramondetta, Lois, additional, Rice, David, additional, Sood, Anil, additional, Tamboli, Pheroze, additional, Thompson, Timothy, additional, Troncoso, Patricia, additional, Tsao, Anne, additional, Wistuba, Ignacio, additional, Carter, Candace, additional, Haydu, Lauren, additional, Hersey, Peter, additional, Jakrot, Valerie, additional, Kakavand, Hojabr, additional, Kefford, Richard, additional, Lee, Kenneth, additional, Long, Georgina, additional, Mann, Graham, additional, Quinn, Michael, additional, Saw, Robyn, additional, Scolyer, Richard, additional, Shannon, Kerwin, additional, Spillane, Andrew, additional, Stretch, Jonathan, additional, Synott, Maria, additional, Thompson, John, additional, Wilmott, James, additional, Al-Ahmadie, Hikmat, additional, Chan, Timothy A., additional, Ghossein, Ronald, additional, Gopalan, Anuradha, additional, Reuter, Victor, additional, Singer, Samuel, additional, Singh, Bhuvanesh, additional, Tien, Nguyen Viet, additional, Broudy, Thomas, additional, Mirsaidi, Cyrus, additional, Nair, Praveen, additional, Drwiega, Paul, additional, Miller, Judy, additional, Smith, Jennifer, additional, Zaren, Howard, additional, Park, Joong-Won, additional, Hung, Nguyen Phi, additional, Kebebew, Electron, additional, Linehan, W. Marston, additional, Metwalli, Adam R., additional, Pacak, Karel, additional, Pinto, Peter A., additional, Schiffman, Mark, additional, Schmidt, Laura S., additional, Vocke, Cathy D., additional, Wentzensen, Nicolas, additional, Worrell, Robert, additional, Yang, Hannah, additional, Moncrieff, Marc, additional, Goparaju, Chandra, additional, Melamed, Jonathan, additional, Pass, Harvey, additional, Botnariuc, Natalia, additional, Caraman, Irina, additional, Cernat, Mircea, additional, Chemencedji, Inga, additional, Clipca, Adrian, additional, Doruc, Serghei, additional, Gorincioi, Ghenadie, additional, Mura, Sergiu, additional, Pirtac, Maria, additional, Stancul, Irina, additional, Tcaciuc, Diana, additional, Albert, Monique, additional, Alexopoulou, Iakovina, additional, Arnaout, Angel, additional, Bartlett, John, additional, Engel, Jay, additional, Gilbert, Sebastien, additional, Parfitt, Jeremy, additional, Sekhon, Harman, additional, Thomas, George, additional, Rassl, Doris M., additional, Rintoul, Robert C., additional, Bifulco, Carlo, additional, Tamakawa, Raina, additional, Urba, Walter, additional, Hayward, Nicholas, additional, Timmers, Henri, additional, Antenucci, Anna, additional, Facciolo, Francesco, additional, Grazi, Gianluca, additional, Marino, Mirella, additional, Merola, Roberta, additional, de Krijger, Ronald, additional, Gimenez-Roqueplo, Anne-Paule, additional, Piché, Alain, additional, Chevalier, Simone, additional, McKercher, Ginette, additional, Birsoy, Kivanc, additional, Barnett, Gene, additional, Brewer, Cathy, additional, Farver, Carol, additional, Naska, Theresa, additional, Pennell, Nathan A., additional, Raymond, Daniel, additional, Schilero, Cathy, additional, Smolenski, Kathy, additional, Williams, Felicia, additional, Morrison, Carl, additional, Borgia, Jeffrey A., additional, Liptay, Michael J., additional, Pool, Mark, additional, Seder, Christopher W., additional, Junker, Kerstin, additional, Dinkin, Mikhail, additional, Manikhas, George, additional, Alvaro, Domenico, additional, Bragazzi, Maria Consiglia, additional, Cardinale, Vincenzo, additional, Carpino, Guido, additional, Gaudio, Eugenio, additional, Chesla, David, additional, Cottingham, Sandra, additional, Dubina, Michael, additional, Moiseenko, Fedor, additional, Dhanasekaran, Renumathy, additional, Becker, Karl-Friedrich, additional, Janssen, Klaus-Peter, additional, Slotta-Huspenina, Julia, additional, Abdel-Rahman, Mohamed H., additional, Aziz, Dina, additional, Bell, Sue, additional, Cebulla, Colleen M., additional, Davis, Amy, additional, Duell, Rebecca, additional, Elder, J. Bradley, additional, Hilty, Joe, additional, Kumar, Bahavna, additional, Lang, James, additional, Lehman, Norman L., additional, Mandt, Randy, additional, Nguyen, Phuong, additional, Pilarski, Robert, additional, Rai, Karan, additional, Schoenfield, Lynn, additional, Senecal, Kelly, additional, Wakely, Paul, additional, Hansen, Paul, additional, Lechan, Ronald, additional, Powers, James, additional, Tischler, Arthur, additional, Grizzle, William E., additional, Sexton, Katherine C., additional, Kastl, Alison, additional, Henderson, Joel, additional, Porten, Sima, additional, Waldmann, Jens, additional, Fassnacht, Martin, additional, Asa, Sylvia L., additional, Schadendorf, Dirk, additional, Couce, Marta, additional, Graefen, Markus, additional, Huland, Hartwig, additional, Sauter, Guido, additional, Schlomm, Thorsten, additional, Simon, Ronald, additional, Tennstedt, Pierre, additional, Olabode, Oluwole, additional, Nelson, Mark, additional, Bathe, Oliver, additional, Carroll, Peter R., additional, Chan, June M., additional, Disaia, Philip, additional, Glenn, Pat, additional, Kelley, Robin K., additional, Landen, Charles N., additional, Phillips, Joanna, additional, Prados, Michael, additional, Simko, Jeffry, additional, Smith-McCune, Karen, additional, VandenBerg, Scott, additional, Roggin, Kevin, additional, Fehrenbach, Ashley, additional, Kendler, Ady, additional, Sifri, Suzanne, additional, Steele, Ruth, additional, Jimeno, Antonio, additional, Carey, Francis, additional, Forgie, Ian, additional, Mannelli, Massimo, additional, Carney, Michael, additional, Hernandez, Brenda, additional, Campos, Benito, additional, Herold-Mende, Christel, additional, Jungk, Christin, additional, Unterberg, Andreas, additional, von Deimling, Andreas, additional, Bossler, Aaron, additional, Galbraith, Joseph, additional, Jacobus, Laura, additional, Knudson, Michael, additional, Knutson, Tina, additional, Ma, Deqin, additional, Milhem, Mohammed, additional, Sigmund, Rita, additional, Godwin, Andrew K., additional, Madan, Rashna, additional, Rosenthal, Howard G., additional, Adebamowo, Clement, additional, Adebamowo, Sally N., additional, Boussioutas, Alex, additional, Beer, David, additional, Giordano, Thomas, additional, Mes-Masson, Anne-Marie, additional, Saad, Fred, additional, Bocklage, Therese, additional, Landrum, Lisa, additional, Mannel, Robert, additional, Moore, Kathleen, additional, Moxley, Katherine, additional, Postier, Russel, additional, Walker, Joan, additional, Zuna, Rosemary, additional, Feldman, Michael, additional, Valdivieso, Federico, additional, Dhir, Rajiv, additional, Luketich, James, additional, Mora Pinero, Edna M., additional, Quintero-Aguilo, Mario, additional, Carlotti, Jr, Carlos Gilberto, additional, Dos Santos, Jose Sebastião, additional, Kemp, Rafael, additional, Sankarankuty, Ajith, additional, Tirapelli, Daniela, additional, Catto, James, additional, Agnew, Kathy, additional, Swisher, Elizabeth, additional, Creaney, Jenette, additional, Robinson, Bruce, additional, Shelley, Carl Simon, additional, Godwin, Eryn M., additional, Kendall, Sara, additional, Shipman, Cassaundra, additional, Bradford, Carol, additional, Carey, Thomas, additional, Haddad, Andrea, additional, Moyer, Jeffey, additional, Peterson, Lisa, additional, Prince, Mark, additional, Rozek, Laura, additional, Wolf, Gregory, additional, Bowman, Rayleen, additional, Fong, Kwun M., additional, Yang, Ian, additional, Korst, Robert, additional, Rathmell, W. Kimryn, additional, Fantacone-Campbell, J. Leigh, additional, Hooke, Jeffrey A., additional, DiPersio, John, additional, Drake, Bettina, additional, Govindan, Ramaswamy, additional, Heath, Sharon, additional, Ley, Timothy, additional, Van Tine, Brian, additional, Westervelt, Peter, additional, Rubin, Mark A., additional, Lee, Jung Il, additional, Aredes, Natália D., additional, and Mariamidze, Armaz, additional

Published
2018
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24. High expression of XIAP and Bcl-2 may inhibit programmed cell death in glioblastomas

Author

Tirapelli, Daniela Pretti da Cunha, primary, Lustosa, Isis Lacrose, additional, Menezes, Sarah Bomfim, additional, Franco, Indira Maynart, additional, Rodrigues, Andressa Romualdo, additional, Peria, Fernanda Maris, additional, Marinho, Alexandre Magno da Nóbrega, additional, Serafini, Luciano Neder, additional, Carlotti Jr, Carlos Gilberto, additional, and Tirapelli, Luís Fernando, additional

Published
2017
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25. High expression of anti-apoptotic genes in grade I and II meningiomas

Author

Tirapelli, Daniela Pretti da Cunha, primary, Menezes, Sarah Bomfim, additional, Franco, Indira Maynart, additional, Lustosa, Isis Lacrose, additional, Rodrigues, Andressa Romualdo, additional, Novais, Paulo Cézar, additional, Santiago, Antônio César Mendes, additional, Peria, Fernanda Maris, additional, Serafini, Luciano Neder, additional, Marinho, Alexandre Magno da Nóbrega, additional, Carlotti Jr, Carlos Gilberto, additional, Colli, Benedicto Oscar, additional, and Tirapelli, Luís Fernando, additional

Published
2017
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27. Analysis of Caspase-9 protein and microRNAs miR-21, miR-126 and miR-155 related to the apoptosis mechanism in the cerebellum of rats submitted to focal cerebral ischemia associated with an alcoholism model.

Author

da SILVA, Jairo Pinheiro, LIZARTE NETO, Fermino Sanches, de Assis CIRINO, Mucio Luiz, de CARVALHO, Camila Albuquerque Melo, CARLOTTI JR., Carlos Gilberto, COLLI, Benedicto Oscar, da Cunha TIRAPELLI, Daniela Pretti, and TIRAPELLI, Luís Fernando

Abstract

Copyright of Arquivos de Neuro-Psiquiatria is the property of Thieme Medical Publishing Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2019
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28. Qualidade de vida e sintomas de ansiedade e depressão em pacientes com tumores cerebrais primários

Author

Bigatão, Marcela dos Reis, Carlotti Jr, Carlos Gilberto, and Carlo, Marysia Mara Rodrigues do Prado de

Subjects
quality of life, qualidade de vida, glioma, depression, depressão, tumor cerebral, Ansiedade, Anxiety, meningioma, brain tumor
Abstract

Objetivo: Avaliar a qualidade de vida e sintomas de ansiedade e depressão em pacientes com diagnóstico de meningioma e glioma de alto grau submetidos à neurocirurgia oncológica. Métodos: Para a coleta de dados, foram aplicados dois instrumentos validados no Brasil: Hospital Anxiety and Depression Scale (HAD) e Item Short-Form Health Survey (SF-36). Resultados: Foram identificadas diferenças estatisticamente significativas quando comparados os dados do SF-36 de ambos os grupos tumorais, no pré e pós-operatório, nos aspectos: capacidade funcional (p = 0,043), aspecto emocional (p = 0,042) e saúde mental (p = 0,042) referente ao grupo meningioma. Quando comparados com respectivos grupos controle, houve diferenças significativas entre os grupos meningioma e controle, nos aspectos físico (p = 0,002) e emocional (p = 0,004), e entre os grupos glioma de alto grau e controle, nos aspectos capacidade funcional (p = 0,003) e físico (p = 0,003). Conclusão: A cirurgia oncológica gerou alterações de humor e na qualidade de vida em ambos os grupos, independente do tipo histológico do tumor. Apesar da relevância do tema, ainda são poucos os estudos sobre o tema. Objective: To evaluate the quality of life, symptoms of anxiety and depression in patients with meningioma and high-grade glioma undergoing oncologic neurosurgery. Methods: Anxiety and Depression Scale (HADS) and Item Short-Form Health Survey (SF-36) Hospital were both applied to collect data. Results: Statistically significant differences were found when comparing the data from the SF-36 in both tumor groups. For the first group (meningioma), the preoperative and postoperative results were: physical functioning (p = 0.043), mental, emotional (p = 0.042) and health (p = 0.042). There were significant differences between the first group (meningioma) and the second group (control groups) in emotional (p = 0.004), physical (p = 0.002) and between the groups of high-grade glioma and control aspects in functional capacity (p = 0.003) and physical capacity (p = 0.003). Conclusion: It was concluded that cancer surgery caused changes in psychological mood and quality of life in both groups, regardless of histological diagnosis type of the tumor and, despite the relevance of the topic, still there are few studies on the topic.

Published
2014

29. High expression of XIAP and Bcl-2 may inhibit programmed cell death in glioblastomas.

Author

da Cunha Tirapelli, Daniela Pretti, Lustosa, Isis Lacrose, Menezes, Sarah Bomfim, Franco, Indira Maynart, Rodrigues, Andressa Romualdo, Peria, Fernanda Maris, da Nóbrega Marinho, Alexandre Magno, Serafini, Luciano Neder, Carlotti Jr, Carlos Gilberto, and Tirapelli, Luís Fernando

Abstract

Copyright of Arquivos de Neuro-Psiquiatria is the property of Thieme Medical Publishing Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2017
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30. High expression of anti-apoptotic genes in grade I and II meningiomas.

Author

da Cunha Tirapelli, Daniela Pretti, Menezes, Sarah Bomfim, Franco, Indira Maynart, Lustosa, Isis Lacrose, Rodrigues, Andressa Romualdo, Novais, Paulo Cézar, Santiago, Antônio César Mendes, Peria, Fernanda Maris, Serafini, Luciano Neder, da Nóbrega Marinho, Alexandre Magno, Carlotti Jr, Carlos Gilberto, Colli, Benedicto Oscar, and Tirapelli, Luís Fernando

Abstract

Copyright of Arquivos de Neuro-Psiquiatria is the property of Thieme Medical Publishing Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2017
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31. Expression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients.

Author

de Sousa, Juliana Ferreira, Torrieri, Raul, Serafim, Rodolfo Bortolozo, Di Cristofaro, Luis Fernando Macedo, Escanfella, Fábio Dalbon, Ribeiro, Rodrigo, Zanette, Dalila Lucíola, Paçó-Larson, Maria Luisa, da Silva Jr, Wilson Araujo, da Cunha Tirapelli, Daniela Pretti, Neder, Luciano, Carlotti Jr, Carlos Gilberto, and Valente, Valeria

Abstract

Astrocytomas are the most common primary brain tumors. They are very resistant to therapies and usually progress rapidly to high-grade lesions. Here, we investigated the potential role of DNA repair genes in astrocytoma progression and resistance. To this aim, we performed a polymerase chain reaction array-based analysis focused on DNA repair genes and searched for correlations between expression patters and survival prognoses. We found 19 genes significantly altered. Combining these genes in all possible arrangements, we found 421 expression signatures strongly associated with poor survival. Importantly, five genes (DDB2, EXO1, NEIL3, BRCA2, and BRIP1) were independently correlated with worse prognoses, revealing single-gene signatures. Moreover, silencing of EXO1, which is remarkably overexpressed, promoted faster restoration of double-strand breaks, while NEIL3 knockdown, also highly overexpressed, caused an increment in DNA damage and cell death after irradiation of glioblastoma cells. These results disclose the importance of DNA repair pathways for the maintenance of genomic stability of high-grade astrocytomas and suggest that EXO1 and NEIL3 overexpression confers more efficiency for double-strand break repair and resistance to reactive oxygen species, respectively. Thereby, we highlight these two genes as potentially related with tumor aggressiveness and promising candidates as novel therapeutic targets. [ABSTRACT FROM AUTHOR]

Published
2017
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32. Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism.

Author

Iozzi Silva, Cristiane, Novais, Paulo Cézar, Rodrigues, Andressa Romualdo, Carvalho, Camila A. M., Colli, Benedicto Oscar, Carlotti Jr., Carlos Gilberto, Tirapelli, Luís Fernando, and Tirapelli, Daniela P. C.

Abstract

Copyright of Arquivos de Neuro-Psiquiatria is the property of Thieme Medical Publishing Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2017
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35. Late-onset social anxiety disorder following traumatic brain injury

Author

Chaves, Cristiano, primary, Trzesniak, Clarissa, additional, Derenusson, Guilherme Nogueira, additional, Araújo, David, additional, Wichert-Ana, Lauro, additional, Machado-de-Sousa, JoÃo Paulo, additional, Carlotti Jr, Carlos Gilberto, additional, Nardi, Antonio E., additional, Zuardi, Antônio W., additional, de S. Crippa, José Alexandre, additional, and Hallak, Jaime E. C., additional

Published
2012
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38. Mesial temporal lobe epilepsy with psychiatric comorbidities: a place for differential neuroinflammatory interplay.

Author

Kandratavicius, Ludmyla, Peixoto-Santos, Jose Eduardo, Monteiro, Mariana Raquel, Scandiuzzi, Renata Caldo, Carlotti Jr., Carlos Gilberto, Assirati Jr., Joao Alberto, Hallak, Jaime Eduardo, and Leite, Joao Pereira

Subjects
KLUVER-Bucy syndrome, CEREBRAL cortex, MENTAL illness, SCHIZOPHRENIA, MEDICAL care
Abstract

Background: Despite the strong association between epilepsy and psychiatric comorbidities, few biological substrates are currently described. We have previously reported neuropathological alterations in mesial temporal lobe epilepsy (MTLE) patients with major depression and psychosis that suggest a morphological and neurochemical basis for psychopathological symptoms. Neuroinflammatory-related structures and molecules might be part of the altered neurochemical milieu underlying the association between epilepsy and psychiatric comorbidities, and such features have not been previously investigated in humans. Methods: MTLE hippocampi of subjects without psychiatric history (MTLEW), MTLE + major depression (MTLE + D), and MTLE + interictal psychosis (MTLE + P) derived from epilepsy surgery and control necropsies were investigated for reactive astrocytes (glial fibrillary acidic protein (GFAP)), activated microglia (human leukocyte antigen, MHC class II (HLA-DR)), glial metallothionein-I/II (MT-I/II), and aquaporin 4 (AQP4) immunohistochemistry. Results: We found an increased GFAP immunoreactive area in the molecular layers, granule cell layer, and cornus ammonis region 2 (CA2) and cornus ammonis region 1 (CA1) of MTLEW and MTLE + P, respectively, compared to MTLE + D. HLA-DR immunoreactive area was higher in cornus ammonis region 3 (CA3) of MTLE + P, compared to MTLE + D and MTLEW, and in the hilus, when compared to MTLEW. MTLEW cases showed increased MT-I/II area in the granule cell layer and CA1, compared to MTLE + P, and in the parasubiculum, when compared to MTLE + D and MTLE + P. Differences between MTLE and control, such as astrogliosis, microgliosis, increased MT-I/II, and decreased perivascular AQP4 in the epileptogenic hippocampus, were in agreement to what is currently described in the literature. Conclusions: Neuroinflammatory-related molecules in MTLE hippocampus show a distinct pattern of expression when patients present with a comorbid psychiatric diagnosis, similar to what is found in the pure forms of schizophrenia and major depression. Future studies focusing on inflammatory characteristics of MTLE with psychiatric comorbidities might help in the design of better therapeutic strategies. [ABSTRACT FROM AUTHOR]

Published
2015
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40. BUB1 and BUBR1 inhibition decreases proliferation and colony formation, and enhances radiation sensitivity in pediatric glioblastoma cells.

Author

Morales, Andressa Gois, Pezuk, Julia Alejandra, Brassesco, María Sol, de Oliveira, Jaqueline Carvalho, de Paula Queiroz, Rosane Gomes, Machado, Hélio Rubens, Carlotti Jr, Carlos Gilberto, Neder, Luciano, de Oliveira, Harley Francisco, Scrideli, Carlos Alberto, and Tone, Luiz Gonzaga

Subjects
TUMORS, CELL culture, RADIATION, CELL growth, GENE expression, APOPTOSIS
Abstract

Purpose: Glioblastoma (GBM) is a very aggressive and lethal brain tumor with poor prognosis. Despite new treatment strategies, patients’ median survival is still lower than 1 year in most cases. The expression of the BUB gene family has demonstrated to be altered in a variety of solid tumors, pointing to a role as putative therapeutic target. The purpose of this study was to determine BUB1, BUB3, and BUBR1 gene expression profiles in glioblastoma and to analyze the effects of BUB1 and BUBR1 inhibition combined or not with Temozolomide and radiation in the pediatric SF188 GBM cell line. Methods: For gene expression analysis, 8 cell lines and 18 tumor samples were used. The effect of BUB1 and BUBR1 inhibition was evaluated using siRNA. Apoptosis, cell proliferation, cell cycle kinetics, micronuclei formation, and clonogenic capacity were analyzed after BUB1 and BUBR1 inhibition. Additionally, combinatorial effects of gene inhibition and radiation or Temozolomide (TMZ) treatment were evaluated through proliferation and clonogenic capacity assays. Results: We report the upregulation of BUB1 and BUBR1 expression and the downregulation of BUB3 in GBM samples and cell lines when compared to white matter samples ( p < 0.05). Decreased cell proliferation and colony formation after BUB1 and BUBR1 inhibition were observed, along with increased micronuclei formation. Combinations with TMZ also caused cell cycle arrest and increased apoptosis. Moreover, our results demonstrate that BUB1 and BUBR1 inhibition sensitized SF188 cells to γ-irradiation as shown by decreased growth and abrogation of colony formation capacity. Conclusion: BUB1 and BUBR1 inhibition decreases proliferation and shows radiosensitizing effects on pediatric GBM cells, which could improve treatment strategies for this devastating tumor. Collectively, these findings highlight the potentials of BUB1 and BUBR1 as putative therapeutic targets for glioblastoma treatment. [ABSTRACT FROM AUTHOR]

Published
2013
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42. Modulation of HJURP (Holliday Junction-Recognizing Protein) Levels Is Correlated with Glioblastoma Cells Survival.

Author

Valente, Valeria, Serafim, Rodolfo Bortolozo, de Oliveira, Leonardo Cesar, Adorni, Fernando Soares, Torrieri, Raul, da Cunha Tirapelli, Daniela Pretti, Espreafico, Enilza Maria, Oba-Shinjo, Sueli Mieko, Marie, Suely Kazue Nagahashi, Paçó-Larson, Maria Luisa, and Carlotti Jr, Carlos Gilberto

Subjects
GLIOBLASTOMA multiforme, CANCER cells, ASTROCYTOMAS, GENE expression, CELLULAR aging, HEALTH risk assessment, EPIDEMIOLOGY, ONCOLOGY
Abstract

Background: Diffuse astrocytomas are the most common type of primary brain cancer in adults. They present a wide variation in differentiation and aggressiveness, being classified into three grades: low-grade diffuse astrocytoma (grade II), anaplastic astrocytoma (grade III) and glioblastoma multiforme (grade IV), the most frequent and the major lethal type. Recent studies have highlighted the molecular heterogeneity of astrocytomas and demonstrated that large-scale analysis of gene expression could help in their classification and treatment. In this context, we previously demonstrated that HJURP, a novel protein involved in the repair of DNA double-strand breaks, is highly overexpressed in glioblastoma. Methodology/Principal Findings: Here we show that HJURP is remarkably overexpressed in a cohort composed of 40 patients with different grade astrocytomas. We also observed that tumors presenting the higher expression levels of HJURP are associated with poor survival prognosis, indicating HJURP overexpression as an independent prognostic factor of death risk for astrocytoma patients. More importantly, we found that HJURP knockdown strongly affects the maintenance of glioblastoma cells in a selective manner. Glioblastoma cells showed remarkable cell cycle arrest and premature senescence that culminated in elevated levels of cell death, differently from non-tumoral cells that were minimally affected. Conclusions: These data suggest that HJURP has an important role in the maintenance of extremely proliferative cells of high-grade gliomas and point to HJURP as a potential therapeutic target for the development of novel treatments for glioma patients. [ABSTRACT FROM AUTHOR]

Published
2013
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43. Microtubule-Associated Proteins in Mesial Temporal Lobe Epilepsy with and without Psychiatric Comorbidities and Their Relation with Granular Cell Layer Dispersion.

Author

Kandratavicius, Ludmyla, Monteiro, Mariana Raquel, Hallak, Jaime Eduardo, Carlotti Jr., Carlos Gilberto, Assirati Jr., Joao Alberto, and Leite, Joao Pereira

Abstract

Background. Despite strong association between epilepsy and psychiatric comorbidities, biological substrates are unknown. We have previously reported decreased mossy fiber sprouting in mesial temporal lobe epilepsy (MTLE) patients with psychosis and increased in those with major depression. Microtubule associated proteins (MAPs) are essentially involved in dendritic and synaptic sprouting. Methods. MTLE hippocampi of subjects without psychiatric history, MTLE + major depression, and MTLE + interictal psychosis derived from epilepsy surgery and control necropsies were investigated for neuronal density, granular layer dispersion, and MAP2 and tau immunohistochemistry. Results. Altered MAP2 and tau expression in MTLE and decreased tau expression in MTLE with psychosis were found. Granular layer dispersion correlated inversely with verbal memory scores, and with MAP2 and tau expression in the entorhinal cortex. Patients taking fluoxetine showed increased neuronal density in the granular layer and those taking haloperidol decreased neuronal density in CA3 and subiculum. Conclusions. Our results indicate relations between MAPs, granular layer dispersion, and memory that have not been previously investigated. Differential MAPs expression in human MTLE hippocampi with and without psychiatric comorbidities suggests that psychopathological states in MTLE rely on differential morphological and possibly neurochemical backgrounds. This clinical study was approved by our institution's Research Ethics Board (HC-FMRP no. 1270/2008) and is registered under the Brazilian National System of Information on Ethics in Human Research (SISNEP) no. 0423.0.004.000-07. [ABSTRACT FROM AUTHOR]

Published
2013
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44. Enhancement of blood-tumor barrier permeability by Sar-[D-Phe8]des-Arg9BK, a metabolically resistant bradykinin B1 agonist, in a rat C6 glioma model.

Author

Cardoso, Ronie Cleverson, Lobão-Soares, Bruno, Bianchin, Marino Muxfeldt, Carlotti Jr., Carlos Gilberto, Walz, Roger, Alvarez-Silva, Márcio, Trentin, Andréa Gonçalves, and Nicolau, Mauro

Subjects
HEMATOMA, BRAIN tumors, BRADYKININ, BLOOD proteins, GLIOMAS
Abstract

Background: While it is well known that bradykinin B2 agonists increase plasma protein extravasation (PPE) in brain tumors, the bradykinin B1 agonists tested thus far are unable to produce this effect. Here we examine the effect of the selective B1 agonist bradykinin (BK) Sar-[DPhe8] des-Arg9BK (SAR), a compound resistant to enzymatic degradation with prolonged activity on PPE in the blood circulation in the C6 rat glioma model. Results: SAR administration significantly enhanced PPE in C6 rat brain glioma compared to saline or BK (p < 0.01). Pre-administration of the bradykinin B1 antagonist [Leu8]-des-Arg (100 nmol/Kg) blocked the SAR-induced PPE in the tumor area. Conclusions: Our data suggest that the B1 receptor modulates PPE in the blood tumor barrier of C6 glioma. A possible role for the use of SAR in the chemotherapy of gliomas deserves further study. [ABSTRACT FROM AUTHOR]

Published
2004
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45. Experimental microaneurysms in rats: II. Comparison between cotton wrapping and microbipolar coagulation

Author

Guerreiro, Nilton Eduardo, Colli, Benedicto Oscar, Carlotti Jr., Carlos Gilberto, and Neder, Luciano

Subjects
*ANEURYSMS, *RATS, *COTTON
Abstract

Background: Microsurgical aneurysm clipping is considered the best treatment for intracranial aneurysms, but often it cannot be used for microaneurysms. These aneurysms may require different type of treatment like bipolar coagulation or wrapping to reinforce the aneurysm wall, but these treatments have no experimentally proven efficacy. In this study the authors analyze the efficacy of 2 methods of treatment to prevent rupture of experimentally induced microaneurysms.Methods: Microaneurysms were induced using mechanical compression of the wall of the aortic bifurcation in 60 rats. The animals were divided in three groups according to aneurysm treatment: Group 1—aneurysms treated using microbipolar coagulation; Group 2—aneurysms wrapped with cotton, and Group 3—no treatment. Four weeks after the treatment, the resistance of the microaneurysm was tested applying intraluminal hyperpressure (up to 2,427 mm Hg). After that, the vessels were microscopically analyzed.Results: No rupture of the aneurysms was observed in Group 2 and all aneurysms ruptured in Groups 1 and 3 during the resistance test. Comparison between the mean bursting pressure of Groups 1 and 3 showed no significant difference (Student''s t test, p > 0.05). The probability of rupture of the microaneurysms was significantly different between Groups 1 and 2, favoring Group 2 (Fisher exact test, p < 0.001).Conclusions: Wrapping of experimental microaneurysms with cotton was effective to protect aneurysm rupture under hyperpressure; and there was no protection using microbipolar coagulation. [Copyright &y& Elsevier]

Published
2004
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46. Experimental microaneurysms in rats: I. Model for induction

Author

Guerreiro, Nilton Eduardo, Colli, Benedicto Oscar, Carlotti Jr., Carlos Gilberto, and Chimelli, Leila

Subjects
*ANEURYSMS, *RATS, *MURIDAE
Abstract

Background: Small aneurysms (lesser than 2 mm) in humans called sessile, baby aneurysms, or microaneurysms, generally are not able to be clipped or to be coil-packed through endovascular route. Among the modalities of treatment that have been used for treating microaneurysms, bipolar coagulation and wrapping of the lesion are outstanding. Nevertheless, demonstration of the efficacy of these treatments is difficult because most reported experimental models for inducting aneurysms are complex and difficult to be reproduced. This study aimed to develop a simple and reproducible model for inducing microaneurysms.Methods: Microaneurysms were induced using a mechanical lesion of the bifurcation of the aorta in 72 rats. Three groups of 10 animals were sacrificed 7, 14, and 21 days after the lesion and 2 groups (35 and 7 animals) after 30 days. The aortic bifurcation was macro/microscopically analyzed in the first 4 groups and a resistance test was applied in the fifth group.Results: Microaneurysms occurred in 77.8% of cases. Microscopically, degenerative changes were observed in the intima, media, and adventitia and in the internal elastic lamina. The bursting pressure ranged from 368 to 1,472 mm Hg during the resistance test in the fifth group.Conclusions: The presented model of experimental microaneurysm induction is simple, reproducible and gives a high rate of positivity. [Copyright &y& Elsevier]

Published
2004
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