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1. MPXV DNA kinetics in bloodstream and other body fluids samples

Author

Meschi, Silvia, Colavita, Francesca, Carletti, Fabrizio, Mazzotta, Valentina, Matusali, Giulia, Specchiarello, Eliana, Ascoli Bartoli, Tommaso, Mondi, Annalisa, Minosse, Claudia, Giancola, Maria Letizia, Pinnetti, Carmela, Valli, Maria Beatrice, Lapa, Daniele, Mizzoni, Klizia, Sullivan, David J., Ou, Jiangda, Focosi, Daniele, Girardi, Enrico, Nicastri, Emanuele, Antinori, Andrea, and Maggi, Fabrizio

Published
2024
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3. Kinetics of viral DNA in body fluids and antibody response in patients with acute Monkeypox virus infection

Author

Colavita, Francesca, Mazzotta, Valentina, Rozera, Gabriella, Abbate, Isabella, Carletti, Fabrizio, Pinnetti, Carmela, Matusali, Giulia, Meschi, Silvia, Mondi, Annalisa, Lapa, Daniele, Vita, Serena, Minosse, Claudia, Aguglia, Camilla, Gagliardini, Roberta, Specchiarello, Eliana, Bettini, Aurora, Nicastri, Emanuele, Girardi, Enrico, Vaia, Francesco, Antinori, Andrea, and Maggi, Fabrizio

Published
2023
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5. SARS-CoV-2 nasopharyngeal viral load in individuals infected with BA.2, compared to Alpha, Gamma, Delta and BA.1 variants: A single-center comparative analysis

Author

Mastrorosa, Ilaria, Cozzi-Lepri, Alessandro, Colavita, Francesca, Lalle, Eleonora, Mazzotta, Valentina, Cimaglia, Claudia, Paulicelli, Jessica, Matusali, Giulia, Fabeni, Lavinia, Carletti, Fabrizio, Rosati, Silvia, Vita, Serena, Giannico, Giuseppina, Piselli, Pierluca, Biliotti, Elisa, Moghazi, Samir Al, Mosti, Silvia, Girardi, Enrico, Nicastri, Emanuele, Garbuglia, Anna Rosa, Maggi, Fabrizio, Vaia, Francesco, and Antinori, Andrea

Published
2022
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7. Infectious Toscana Virus in Seminal Fluid of Young Man Returning from Elba Island, Italy

Author

Matusali, Giulia, D'Abramo, Alessandra, Terrosi, Chiara, Carletti, Fabrizio, Colavita, Francesca, Vairo, Francesco, Savellini, Gianni Gori, Gandolfo, Claudia, Anichini, Gabriele, Lalle, Eleonora, Bordi, Licia, Corpolongo, Angela, Maritti, Micaela, Marchioni, Luisa, Capobianchi, Maria Rosaria, Castilletti, Concetta, Cusi, Maria Grazia, and Nicastri, Emanuele

Subjects
Semen -- Case studies -- Health aspects, Bunyaviruses -- Case studies -- Identification and classification, RNA virus infections -- Case studies -- Risk factors -- Diagnosis, Viral meningitis -- Case studies -- Risk factors -- Diagnosis, Health
Abstract

Toscana virus (TOSV) is an arthropodborne virus, belonging to the genus Phlebovirus, that was first isolated in Monte Argentario in 1971 from the sand flies Phlebotomus perniciosus and P. perfiliewi [...]

Published
2022
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8. Risk and predictive factors of prolonged viral RNA shedding in upper respiratory specimens in a large cohort of COVID-19 patients admitted to an Italian reference hospital

Author

Abbonizio, Maria Alessandra, Abdeddaim, Amina, Agostini, Elisabetta, Agrati, Chiara, Albarello, Fabrizio, Amadei, Gioia, Amendola, Alessandra, Antinori, Andrea, Antonica, Maria Assunta, Antonini, Mario, Bartoli, Tommaso Ascoli, Baldini, Francesco, Barbaro, Raffaella, Bartolini, Barbara, Bellagamba, Rita, Benigni, Martina, Bevilacqua, Nazario, Biava, Gianluigi, Bibas, Michele, Bordi, Licia, Bordoni, Veronica, Boumis, Evangelo, Branca, Marta, Buonomo, Rosanna, Busso, Donatella, Camici, Marta, Campioni, Paolo, Canichella, Flaminia, Capobianchi, Maria Rosaria, Capone, Alessandro, Caporale, Cinzia, Caraffa, Emanuela, Caravella, Ilaria, Carletti, Fabrizio, Castilletti, Concetta, Cataldo, Adriana, Cerilli, Stefano, Cerva, Carlotta, Chiappini, Roberta, Chinello, Pierangelo, Cianfarani, Maria Assunta, Ciaralli, Carmine, Cimaglia, Claudia, Cinicola, Nicola, Ciotti, Veronica, Cicalini, Stefania, Colavita, Francesca, Corpolongo, Angela, Cristofaro, Massimo, Curiale, Salvatore, D’Abramo, Alessandra, Dantimi, Cristina, De Angelis, Alessia, De Angelis, Giada, De Palo, Maria Grazia, De Zottis, Federico, Di Bari, Virginia, Di Lorenzo, Rachele, Di Stefano, Federica, D’Offizi, Gianpiero, Donno, Davide, Evangelista, Francesca, Faraglia, Francesca, Farina, Anna, Ferraro, Federica, Fiorentini, Lorena, Frustaci, Andrea, Fusetti, Matteo, Galati, Vincenzo, Gagliardini, Roberta, Gallì, Paola, Garotto, Gabriele, Gaviano, Ilaria, Tekle, Saba Gebremeskel, Giancola, Maria Letizia, Giansante, Filippo, Giombini, Emanuela, Granata, Guido, Greci, Maria Cristina, Grilli, Elisabetta, Grisetti, Susanna, Gualano, Gina, Iacomi, Fabio, Iaconi, Marta, Iannicelli, Giuseppina, Inversi, Carlo, Ippolito, Giuseppe, Lalle, Eleonora, Lamanna, Maria Elena, Lanini, Simone, Lapa, Daniele, Lepore, Luciana, Libertone, Raffaella, Lionetti, Raffaella, Liuzzi, Giuseppina, Loiacono, Laura, Lucia, Andrea, Lufrani, Franco, Macchione, Manuela, Maffongelli, Gaetano, Marani, Alessandra, Marchioni, Luisa, Mariano, Andrea, Marini, Maria Cristina, Maritti, Micaela, Mastrobattista, Annelisa, Mastrorosa, Ilaria, Matusali, Giulia, Mazzotta, Valentina, Mencarini, Paola, Meschi, Silvia, Messina, Francesco, Micarelli, Sibiana, Mogavero, Giulia, Mondi, Annalisa, Montalbano, Marzia, Montaldo, Chiara, Mosti, Silvia, Murachelli, Silvia, Musso, Maria, Nardi, Michela, Navarra, Assunta, Nicastri, Emanuele, Nocioni, Martina, Noto, Pasquale, Noto, Roberto, Oliva, Alessandra, Onnis, Ilaria, Ottou, Sandrine, Palazzolo, Claudia, Pallini, Emanuele, Palmieri, Fabrizio, Palombi, Giulio, Pareo, Carlo, Passeri, Virgilio, Pelliccioni, Federico, Penna, Giovanna, Petrecchia, Antonella, Petrone, Ada, Petrosillo, Nicola, Pianura, Elisa, Pinnetti, Carmela, Pisciotta, Maria, Piselli, Pierluca, Pittalis, Silvia, Pontarelli, Agostina, Proietti, Costanza, Puro, Vincenzo, Ramazzini, Paolo Migliorisi, Rianda, Alessia, Rinonapoli, Gabriele, Rosati, Silvia, Rubino, Dorotea, Rueca, Martina, Ruggeri, Alberto, Sacchi, Alessandra, Sampaolesi, Alessandro, Sanasi, Francesco, Santagata, Carmen, Scarabello, Alessandra, Scarcia, Silvana, Schininà, Vincenzo, Scognamiglio, Paola, Scorzolini, Laura, Stazi, Giulia, Strano, Giacomo, Taglietti, Fabrizio, Taibi, Chiara, Taloni, Giorgia, Nardi, Tetaj, Tonnarini, Roberto, Topino, Simone, Tozzi, Martina, Vaia, Francesco, Vairo, Francesco, Valli, Maria Beatrice, Vergori, Alessandra, Vincenzi, Laura, Visco-Comandini, Ubaldo, Vita, Serena, Vittozzi, Pietro, Zaccarelli, Mauro, Zanetti, Antonella, Zito, Sara, Lorenzini, Patrizia, Di Gennaro, Francesco, and Girardi, Enrico

Published
2021
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9. Torquetenovirus Viremia Quantification Using Real-Time PCR Developed on a Fully Automated, Random-Access Platform.

Author

Spezia, Pietro Giorgio, Carletti, Fabrizio, Novazzi, Federica, Specchiarello, Eliana, Genoni, Angelo, Drago Ferrante, Francesca, Minosse, Claudia, Matusali, Giulia, Mancini, Nicasio, Focosi, Daniele, Antonelli, Guido, Girardi, Enrico, and Maggi, Fabrizio

Subjects
*VIREMIA, *TURNAROUND time, *CLINICAL pathology, *TRANSPLANTATION of organs, tissues, etc., *IMMUNITY, *TACROLIMUS
Abstract

Quantification of Torquetenovirus (TTV) viremia is becoming important for evaluating the status of the immune system in solid organ transplant recipients, monitoring the appearance of post-transplant complications, and controlling the efficacy of maintenance immunosuppressive therapy. Thus, diagnostic approaches able to scale up TTV quantification are needed. Here, we report on the development and validation of a real-time PCR assay for TTV quantification on the Hologic Panther Fusion® System by utilizing its open-access channel. The manual real-time PCR previously developed in our laboratories was optimized to detect TTV DNA on the Hologic Panther Fusion® System. The assay was validated using clinical samples. The automated TTV assay has a limit of detection of 1.6 log copies per ml of serum. Using 112 samples previously tested via manual real-time PCR, the concordance in TTV detection was 93% between the assays. When the TTV levels were compared, the overall agreement between the methods, as assessed using Passing–Bablok linear regression and Bland–Altman analyses, was excellent. In summary, we validated a highly sensitive and accurate method for the diagnostic use of TTV quantification on a fully automated Hologic Panther Fusion® System. This will greatly improve the turnaround time for TTV testing and better support the laboratory diagnosis of this new viral biomarker. [ABSTRACT FROM AUTHOR]

Published
2024
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10. Rapid Determination of SARS-CoV-2 Integrity and Infectivity by Using Propidium Monoazide Coupled with Digital Droplet PCR.

Author

Sberna, Giuseppe, Mija, Cosmina, Lalle, Eleonora, Rozera, Gabriella, Matusali, Giulia, Carletti, Fabrizio, Girardi, Enrico, and Maggi, Fabrizio

Subjects
PROPIDIUM monoazide, SARS-CoV-2, VIRAL load, COVID-19 pandemic, CIRCULATING tumor DNA
Abstract

SARS-CoV-2 is a highly infectious virus responsible for the COVID-19 pandemic. Therefore, it is important to assess the risk of SARS-CoV-2 infection, especially in persistently positive patients. Rapid discrimination between infectious and non-infectious viruses aids in determining whether prevention, control, and treatment measures are necessary. For this purpose, a method was developed and utilized involving a pre-treatment with 50 µM of propidium monoazide (PMAxx, a DNA intercalant) combined with a digital droplet PCR (ddPCR). The ddPCR method was performed on 40 nasopharyngeal swabs (NPSs) both before and after treatment with PMAxx, revealing a reduction in the viral load at a mean of 0.9 Log copies/mL (SD ± 0.6 Log copies/mL). Furthermore, six samples were stratified based on the Ct values of SARS-CoV-2 RNA (Ct < 20, 20 < Ct < 30, Ct > 30) and analyzed to compare the results obtained via a ddPCR with viral isolation and a negative-chain PCR. Of the five samples found positive via a ddPCR after the PMAxx treatment, two of the samples showed the highest post-treatment SARS-CoV-2 loads. The virus was isolated in vitro from both samples and the negative strand chains were detected. In three NPS samples, SARS CoV-2 was present post-treatment at a low level; it was not isolated in vitro, and, when detected, the strand was negative. Our results indicate that the established method is useful for determining whether the SARS-CoV-2 within positive NPS samples is intact and capable of causing infection. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Molnupiravir increases SARS‐CoV‐2 genome diversity and complexity: A case‐control cohort study.

Author

Gruber, Cesare Ernesto Maria, Tucci, Fabio Giovanni, Giombini, Emanuela, Mazzotta, Valentina, Spezia, Pietro Giorgio, Rueca, Martina, Mastrorosa, Ilaria, Fabeni, Lavinia, Berno, Giulia, Butera, Ornella, Rosati, Silvia, Specchiarello, Eliana, Carletti, Fabrizio, Focosi, Daniele, Nicastri, Emanuele, Girardi, Enrico, Antinori, Andrea, and Maggi, Fabrizio

Subjects
MOLNUPIRAVIR, SARS-CoV-2, WHOLE genome sequencing, COVID-19 pandemic, APOLIPOPROTEIN B
Abstract

Molnupiravir, an oral direct‐acting antiviral effective in vitro against SARS‐CoV‐2, has been largely employed during the COVID‐19 pandemic, since December 2021. After marketing and widespread usage, a progressive increase in SARS‐CoV‐2 lineages characterized by a higher transition/transversion ratio, a characteristic signature of molnupiravir action, appeared in the Global Initiative on Sharing All Influenza Data (GISAID) and International Nucleotide Sequence Database Collaboration (INSDC) databases. Here, we assessed the drug effects by SARS‐CoV‐2 whole‐genome sequencing on 38 molnupiravir‐treated persistently positive COVID‐19 outpatients tested before and after treatment. Seventeen tixagevimab/cilgavimab‐treated outpatients served as controls. Mutational analyses confirmed that SARS‐CoV‐2 exhibits an increased transition/transversion ratio seven days after initiation of molnupiravir. Moreover we observed an increased G‐>A ratio compared to controls, which was not related to apolipoprotein B mRNAediting enzyme, catalytic polypeptide‐like (APOBEC) activity. In addition, we demonstrated for the first time an increased diversity and complexity of the viral quasispecies. [ABSTRACT FROM AUTHOR]

Published
2024
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13. 2019-novel Coronavirus severe adult respiratory distress syndrome in two cases in Italy: An uncommon radiological presentation

Author

Abbonizio, Maria Alessandra, Agrati, Chiara, Albarello, Fabrizio, Amadei, Gioia, Amendola, Alessandra, Antonini, Mario, Barbaro, Raffaella, Bartolini, Barbara, Benigni, Martina, Bevilacqua, Nazario, Bordi, Licia, Bordoni, Veronica, Branca, Marta, Campioni, Paolo, Capobianchi, Maria Rosaria, Caporale, Cinzia, Caravella, Ilaria, Carletti, Fabrizio, Castilletti, Concetta, Chiappini, Roberta, Ciaralli, Carmine, Colavita, Francesca, Corpolongo, Angela, Cristofaro, Massimo, Curiale, Salvatore, D’Abramo, Alessandra, Dantimi, Cristina, Angelis, Alessia De, Angelis, Giada De, Lorenzo, Rachele Di, Stefano, Federica Di, Ferraro, Federica, Fiorentini, Lorena, Frustaci, Andrea, Gallì, Paola, Garotto, Gabriele, Giancola, Maria Letizia, Giansante, Filippo, Giombini, Emanuela, Greci, Maria Cristina, Ippolito, Giuseppe, Lalle, Eleonora, Lanini, Simone, Lapa, Daniele, Lepore, Luciana, Lucia, Andrea, Lufrani, Franco, Macchione, Manuela, Marani, Alessandra, Marchioni, Luisa, Mariano, Andrea, Marini, Maria Cristina, Maritti, Micaela, Matusali, Giulia, Meschi, Silvia, Montaldo, Francesco Messina Chiara, Murachelli, Silvia, Nicastri, Emanuele, Noto, Roberto, Palazzolo, Claudia, Pallini, Emanuele, Passeri, Virgilio, Pelliccioni, Federico, Petrecchia, Antonella, Petrone, Ada, Petrosillo, Nicola, Pianura, Elisa, Pisciotta, Maria, Pittalis, Silvia, Proietti, Costanza, Puro, Vincenzo, Rinonapoli, Gabriele, Rueca, Martina, Sacchi, Alessandra, Sanasi, Francesco, Santagata, Carmen, Scarcia, Silvana, Schininà, Vincenzo, Scognamiglio, Paola, Scorzolini, Laura, Stazi, Giulia, Vaia, Francesco, Vairo, Francesco, Valli, Maria Beatrice, Di Stefano, Federica, Eskild, Petersen, and Zumla, Alimuddin

Published
2020
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14. SARS-CoV-2 Phylogenetic Analysis, Lazio Region, Italy, February-March 2020

Author

Bartolini, Barbara, Rueca, Martina, Gruber, Cesare Ernesto Maria, Messina, Francesco, Carletti, Fabrizio, Giombini, Emanuela, Lalle, Eleonora, Bordi, Licia, Matusali, Giulia, Colavita, Francesca, Castilletti, Concetta, Vairo, Francesco, Ippolito, Giuseppe, Capobianchi, Maria Rosaria, and Caro, Antonino Di

Subjects
Severe acute respiratory syndrome -- Health aspects -- Analysis, Coronaviruses -- Analysis -- Health aspects, Phylogeny -- Analysis -- Health aspects, Epidemiology -- Analysis -- Health aspects, COVID-19 -- Analysis -- Health aspects, Health
Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has raised serious concerns because of its rapid dissemination worldwide. Italy is one of the countries with the highest number of coronavirus disease [...]

Published
2020
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15. Kinetics of TTV Loads in Peripheral Blood Mononuclear Cells of Early Treated Acute HIV Infections

Author

Abbate, Isabella, primary, Rozera, Gabriella, additional, Cimini, Eleonora, additional, Carletti, Fabrizio, additional, Tartaglia, Eleonora, additional, Rubino, Marika, additional, Pittalis, Silvia, additional, Esvan, Rozenn, additional, Gagliardini, Roberta, additional, Mondi, Annalisa, additional, Mazzotta, Valentina, additional, Camici, Marta, additional, Girardi, Enrico, additional, Vaia, Francesco, additional, Puro, Vincenzo, additional, Antinori, Andrea, additional, and Maggi, Fabrizio, additional

Published
2023
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16. A Case of Severe Mpox Complicated with Streptococcus pyogenes Sepsis in a Patient with HIV Infection

Author

Di Bari, Silvia, primary, Mondi, Annalisa, additional, Pinnetti, Carmela, additional, Mazzotta, Valentina, additional, Carletti, Fabrizio, additional, Matusali, Giulia, additional, Vincenti, Donatella, additional, Gagliardini, Roberta, additional, Santoro, Raffaele, additional, Fontana, Carla, additional, Maggi, Fabrizio, additional, Girardi, Enrico, additional, Vaia, Francesco, additional, and Antinori, Andrea, additional

Published
2023
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17. Effect of tecovirimat on healing time and viral clearance by emulation of a target trial in patients hospitalized for mpox

Author

Mazzotta, Valentina, primary, Cozzi‐Lepri, Alessandro, additional, Lanini, Simone, additional, Mondi, Annalisa, additional, Carletti, Fabrizio, additional, Tavelli, Alessandro, additional, Gagliardini, Roberta, additional, Vita, Serena, additional, Pinnetti, Carmela, additional, Aguglia, Camilla, additional, Colavita, Francesca, additional, Faccendini, Paolo, additional, Matusali, Giulia, additional, Faraglia, Francesca, additional, Beccacece, Alessia, additional, Paulicelli, Jessica, additional, Girardi, Enrico, additional, Nicastri, Emanuele, additional, Vaia, Francesco, additional, Maggi, Fabrizio, additional, and Antinori, Andrea, additional

Published
2023
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18. Evolution of SARS‐CoV‐2 variants of concern over a period of Delta and Omicron cocirculation, among patients hospitalized for COVID‐19 in an Italian reference hospital: Impact on clinical outcomes

Author

Mondi, Annalisa, primary, Mastrorosa, Ilaria, additional, Piselli, Pierluca, additional, Cimaglia, Claudia, additional, Matusali, Giulia, additional, Carletti, Fabrizio, additional, Giannico, Giuseppina, additional, Milozzi, Eugenia, additional, Biliotti, Elisa, additional, Di Bari, Silvia, additional, Chinello, Pierangelo, additional, Beccacece, Alessia, additional, Faraglia, Francesca, additional, Vittozzi, Pietro, additional, Mosti, Silvia, additional, Tetaj, Nardi, additional, Stazi, Giulia Valeria, additional, Pinnetti, Carmela, additional, Camici, Marta, additional, D'Annunzio, Alberto, additional, Marani, Alessandra, additional, Fabeni, Lavinia, additional, Specchiarello, Eliana, additional, Gruber, Cesare Ernesto Maria, additional, Villanacci, Alberta, additional, Minicucci, Sabrina, additional, Garbuglia, Anna Rosa, additional, Ianniello, Stefania, additional, Marchioni, Luisa, additional, Taglietti, Fabrizio, additional, D'Offizi, Gianpiero, additional, Palmieri, Fabrizio, additional, Nicastri, Emanuele, additional, Maggi, Fabrizio, additional, Vaia, Francesco, additional, Girardi, Enrico, additional, and Antinori, Andrea, additional

Published
2023
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19. Temporal intra‐host variability of mpox virus genomes in multiple body tissues

Author

Rueca, Martina, primary, Tucci, Fabio Giovanni, additional, Mazzotta, Valentina, additional, Gramigna, Giulia, additional, Gruber, Cesare Ernesto Maria, additional, Fabeni, Lavinia, additional, Giombini, Emanuela, additional, Matusali, Giulia, additional, Pinnetti, Carmela, additional, Mariano, Andrea, additional, Butera, Ornella, additional, Specchiarello, Eliana, additional, Mondi, Annalisa, additional, Lanini, Simone, additional, Carletti, Fabrizio, additional, Girardi, Enrico, additional, Vaia, Francesco, additional, Nicastri, Emanuele, additional, Antinori, Andrea, additional, and Maggi, Fabrizio, additional

Published
2023
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20. Pharyngo-tonsillar involvement of Mpox in a cohort of men who have sex with men (MSM): A serious risk of missing diagnosis.

Author

Pinnetti, Carmela, Mondi, Annalisa, Mazzotta, Valentina, Vita, Serena, Carletti, Fabrizio, Aguglia, Camilla, Beccacece, Alessia, Vergori, Alessandra, Gagliardini, Roberta, Specchiarello, Eliana, Ascoli Bartoli, Tommaso, Baldini, Francesco, Giancola, Maria Letizia, Valli, Maria Beatrice, D'Abramo, Alessandra, Gebremeskel Teklè, Saba, Fontana, Carla, Garbuglia, Anna Rosa, Girardi, Enrico, and Maggi, Fabrizio

Abstract

During the 2022-outbreak, peculiar clinical presentations of Mpox have been described, some of which can make the diagnosis of the disease extremely challenging. Here we report a case series of fourteen patients with Mpox pharynogotonsillar involvement (PTI) seen at National Institute for Infectious Diseases, " Lazzaro Spallanzani ", in Rome, Italy from May to September 2022. All included patients were men who have sex with men (median age 38 years) reporting unprotected sex within three weeks from symptoms onset. Seven out of fourteen patients needed hospitalization due to uncontrolled pain, reduced airspace and difficulty swallowing, of whom five were effectively treated with tecovirimat or cidofovir. The remaining two patients were treated with symptomatic drugs. The typical Mpox muco-cutaneous manifestations were not observed simultaneously with PTI in three patients, two of whom developed the lesions after several days, while one never manifested them. Polymerase Chain Reaction (PCR) for Mpox virus was positive in oropharyngeal swab, saliva and serum. Although PTI occurs in only a small percentage of Mpox cases, its diagnosis is of utmost importance. In fact, this localization, if not identified, could lead to serious complications in the absence of early antiviral treatment and to missed diagnosis with an increased risk of disease transmission. [ABSTRACT FROM AUTHOR]

Published
2024
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21. Outbreaks of autochthonous Dengue in Lazio region, Italy, August to September 2023: preliminary investigation.

Author

De Carli, Gabriella, Carletti, Fabrizio, Spaziante, Martina, Maria Gruber, Cesare Ernesto, Rueca, Martina, Spezia, Pietro Giorgio, Vantaggio, Valentina, Barca, Alessandra, De Liberato, Claudio, Romiti, Federico, Scicluna, Maria Teresa, Vaglio, Stefania, Feccia, Mariano, Di Rosa, Enrico, Gianzi, Francesco Paolo, Giambi, Cristina, Scognamiglio, Paola, Nicastri, Emanuele, Girardi, Enrico, and Maggi, Fabrizio

Published
2023
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22. Analysis of Diagnostic Findings From the European Mobile Laboratory in Guéckédou, Guinea, March 2014 Through March 2015

Author

Kerber, Romy, Krumkamp, Ralf, Diallo, Boubacar, Jaeger, Anna, Rudolf, Martin, Lanini, Simone, Bore, Joseph Akoi, Koundouno, Fara Raymond, Becker-Ziaja, Beate, Fleischmann, Erna, Stoecker, Kilian, Meschi, Silvia, Mély, Stéphane, Newman, Edmund N. C., Carletti, Fabrizio, Portmann, Jasmine, Korva, Misa, Wolff, Svenja, Molkenthin, Peter, Kis, Zoltan, Kelterbaum, Anne, Bocquin, Anne, Strecker, Thomas, Fizet, Alexandra, Castilletti, Concetta, Schudt, Gordian, Ottowell, Lisa, Kurth, Andreas, Atkinson, Barry, Badusche, Marlis, Cannas, Angela, Pallasch, Elisa, Bosworth, Andrew, Yue, Constanze, Pályi, Bernadett, Ellerbrok, Heinz, Kohl, Claudia, Oestereich, Lisa, Logue, Christopher H., Lüdtke, Anja, Richter, Martin, Ngabo, Didier, Borremans, Benny, Becker, Dirk, Gryseels, Sophie, Abdellati, Saïd, Vermoesen, Tine, Kuisma, Eeva, Kraus, Annette, Liedigk, Britta, Maes, Piet, Thom, Ruth, Duraffour, Sophie, Diederich, Sandra, Hinzmann, Julia, Afrough, Babak, Repits, Johanna, Mertens, Marc, Vitoriano, Inês, Bah, Amadou, Sachse, Andreas, Boettcher, Jan Peter, Wurr, Stephanie, Bockholt, Sabrina, Nitsche, Andreas, Županc, Tatjana Avšič, Strasser, Marc, Ippolito, Giuseppe, Becker, Stephan, Raoul, Herve, Carroll, Miles W., De Clerck, Hilde, Van Herp, Michel, Sprecher, Armand, Koivogui, Lamine, Magassouba, N'Faly, Keïta, Sakoba, Drury, Patrick, Gurry, Cèline, Formenty, Pierre, May, Jürgen, Gabriel, Martin, Wölfel, Roman, Günther, Stephan, and Di Caro, Antonino

Published
2016

23. Clinical experience with use of oral Tecovirimat or Intravenous Cidofovir for the treatment of Monkeypox in an Italian reference hospital

Author

Mondi, Annalisa, primary, Gagliardini, Roberta, additional, Mazzotta, Valentina, additional, Vita, Serena, additional, Carletti, Fabrizio, additional, Pinnetti, Carmela, additional, Giancola, Maria Letizia, additional, Specchiarello, Eliana, additional, Lanini, Simone, additional, Faraglia, Francesca, additional, Minosse, Claudia, additional, Paulicelli, Jessica, additional, Mariano, Andrea, additional, Rozera, Gabriella, additional, Fontana, Carla, additional, Faccendini, Paolo, additional, Maggi, Fabrizio, additional, Girardi, Enrico, additional, Vaia, Francesco, additional, Nicastri, Emanuele, additional, and Antinori, Andrea, additional

Published
2023
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25. External quality assessment of SARS-CoV-2 serology in European expert laboratories, April 2021

Author

Mögling, Ramona, Colavita, Francesca, Reimerink, Johan, Melidou, Angeliki, Leitmeyer, Katrin, Keramarou, Maria, Lapa, Daniele, Francalancia, Massimo, Murk, Jean-Luc, Vossen, Ann, Carletti, Fabrizio, Hogema, Boris, Meijer, Adam, Deprez, Liesbet, Di Caro, Antonino, Castilletti, Concetta, and Reusken, Chantal Bem

Subjects
Europe, SARS-CoV-2, COVID-19, EQA, antibodies, serology
Published
2022

26. External quality assessment of SARS-CoV-2 serology in European expert laboratories, April 2021

Author

Mögling, Ramona, primary, Colavita, Francesca, additional, Reimerink, Johan, additional, Melidou, Angeliki, additional, Leitmeyer, Katrin, additional, Keramarou, Maria, additional, Lapa, Daniele, additional, Francalancia, Massimo, additional, Murk, Jean-Luc, additional, Vossen, Ann, additional, Carletti, Fabrizio, additional, Hogema, Boris, additional, Meijer, Adam, additional, Deprez, Liesbet, additional, di Caro, Antonino, additional, Castilletti, Concetta, additional, and Reusken, Chantal BEM, additional

Published
2022
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27. Monkeypox virus isolation from a semen sample collected in the early phase of infection in a patient with prolonged seminal viral shedding

Author

Lapa, Daniele, primary, Carletti, Fabrizio, additional, Mazzotta, Valentina, additional, Matusali, Giulia, additional, Pinnetti, Carmela, additional, Meschi, Silvia, additional, Gagliardini, Roberta, additional, Colavita, Francesca, additional, Mondi, Annalisa, additional, Minosse, Claudia, additional, Scorzolini, Laura, additional, Cicalini, Stefania, additional, Maffongelli, Gaetano, additional, Specchiarello, Eliana, additional, Camici, Marta, additional, Bettini, Aurora, additional, Baldini, Francesco, additional, Francalancia, Massimo, additional, Mizzoni, Klizia, additional, Garbuglia, Anna Rosa, additional, Nicastri, Emanuele, additional, Girardi, Enrico, additional, Antinori, Andrea, additional, Vaia, Francesco, additional, and Maggi, Fabrizio, additional

Published
2022
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28. Passengers screening for SARS-CoV-2 infection at Rome Fiumicino Airport: a strategy aimed at limiting the spreading of the infection through COVID-free air flights revealed the airport role as a formidable sentinel center for monitoring pandemic trends and viral variants circulation

Author

Amendola, Alessandra, primary, Minosse, Claudia, additional, Carletti, Fabrizio, additional, Specchiarello, Elena, additional, Lalle, Eleonora, additional, Bordi, Licia, additional, Castilletti, Concetta, additional, Forbici, Federica, additional, Pisciotta, Maria, additional, Rossi, Fabrizio, additional, Scarparo, Tiziano, additional, Garbuglia, Anna Rosa, additional, and Vaia, Francesco, additional

Published
2022
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29. Molecular Characterization of Autochthonous Chikungunya Cluster in Latium Region, Italy

Author

Bordi, Licia, Carletti, Fabrizio, Lalle, Eleonora, Colavita, Francesca, Meschi, Silvia, Caro, Antonino Di, Nicastri, Emanuele, Scognamiglio, Paola, Vairo, Francesco, Lallo, Domenico Di, Panella, Vincenzo, Capobianchi, Maria R., Ippolito, Giuseppe, and Castilletti, Concetta

Subjects
Mosquitoes -- Health aspects, Epidemics -- Health aspects, Infection -- Health aspects, Health
Abstract

Local transmission of chikungunya virus (CHIKV) has been confirmed in the Lazio region of Italy, with 2 related autochthonous clusters in the cities of Anzio and Rome (1). This event [...]

Published
2018
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30. Detection of SARS-CoV-2 Variants via Different Diagnostics Assays Based on Single-Nucleotide Polymorphism Analysis.

Author

Specchiarello, Eliana, Matusali, Giulia, Carletti, Fabrizio, Gruber, Cesare Ernesto Maria, Fabeni, Lavinia, Minosse, Claudia, Giombini, Emanuela, Rueca, Martina, Maggi, Fabrizio, Amendola, Alessandra, and Garbuglia, Anna Rosa

Subjects
SARS-CoV-2, SARS-CoV-2 Delta variant, SARS-CoV-2 Omicron variant, SINGLE nucleotide polymorphisms
Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is characterized by fast evolution with the appearance of several variants. Next-Generation Sequencing (NGS) technology is considered the gold standard for monitoring known and new SARS-CoV-2 variants. However, the complexity of this technology renders this approach impracticable in laboratories located in areas with limited resources. We analyzed the capability of the ThermoFisher TaqPath COVID-19 RT-PCR (TaqPath) and the Seegene Novaplex SARS-CoV-2 Variant assay (Novaplex) to detect Omicron variants; the Allplex VariantII (Allplex) was also evaluated for Delta variants. Sanger sequencing (SaS) was the reference method. The results obtained with n = 355 nasopharyngeal samples were: negative with TaqPath, although positive with other qualitative molecular assays (n = 35); undetermined (n = 40) with both the assays; negative for the ∆69/70 mutation and confirmed as the Delta variant via SaS (n = 100); positive for ∆69/70 and confirmed as Omicron BA.1 via SaS (n = 80); negative for ∆69/70 and typed as Omicron BA.2 via SaS (n = 80). Novaplex typed 27.5% of samples as undetermined with TaqPath, 11.4% of samples as negative with TaqPath, and confirmed 100% of samples were Omicron subtypes. In total, 99/100 samples were confirmed as the Delta variant with Allplex with a positive per cent agreement (PPA) of 98% compared to SaS. As undermined samples with Novaplex showed RdRp median Ct values (Ct = 35.4) statistically higher than those of typed samples (median Ct value = 22.0; p < 0.0001, Mann–Whitney test), the inability to establish SARS-CoV-2 variants was probably linked to the low viral load. No amplification was obtained with SaS among all 35 negative TaqPath samples. Overall, 20% of samples which were typed as negative or undetermined with TaqPath, and among them, twelve were not typed even by SaS, but they were instead correctly identified with Novaplex. Although full-genome sequencing remains the elected method to characterize new strains, our data show the high ability of a SNP-based assay to identify VOCs, also resolving samples typed as undetermined with TaqPath. [ABSTRACT FROM AUTHOR]

Published
2023
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31. Unique human immune signature of Ebola virus disease in Guinea

Author

Ruibal, Paula, Oestereich, Lisa, Ldtke, Anja, Becker-Ziaja, Beate, Wozniak, David M., Kerber, Romy, Korva, Mia, Cabeza-Cabrerizo, Mar, Bore, Joseph A., Koundouno, Fara Raymond, Duraffour, Sophie, Weller, Romy, Thorenz, Anja, Cimini, Eleonora, Viola, Domenico, Agrati, Chiara, Repits, Johanna, Afrough, Babak, Cowley, Lauren A., Ngabo, Didier, Hinzmann, Julia, Mertens, Marc, Vitoriano, Ins, Logue, Christopher H., Boettcher, Jan Peter, Pallasch, Elisa, Sachse, Andreas, Bah, Amadou, Nitzsche, Katja, Kuisma, Eeva, Michel, Janine, Holm, Tobias, Zekeng, Elsa-Gayle, Garca-Dorival, Isabel, Wlfel, Roman, Stoecker, Kilian, Fleischmann, Erna, Strecker, Thomas, Di Caro, Antonino, Avi-upanc, Tatjana, Kurth, Andreas, Meschi, Silvia, Mly, Stephane, Newman, Edmund, Bocquin, Anne, Kis, Zoltan, Kelterbaum, Anne, Molkenthin, Peter, Carletti, Fabrizio, Portmann, Jasmine, Wolff, Svenja, Castilletti, Concetta, Schudt, Gordian, Fizet, Alexandra, Ottowell, Lisa J., Herker, Eva, Jacobs, Thomas, Kretschmer, Birte, Severi, Ettore, Ouedraogo, Nobila, Lago, Mar, Negredo, Anabel, Franco, Leticia, Anda, Pedro, Schmiedel, Stefan, Kreuels, Benno, Wichmann, Dominic, Addo, Marylyn M., Lohse, Ansgar W., De Clerck, Hilde, Nanclares, Carolina, Jonckheere, Sylvie, Van Herp, Michel, Sprecher, Armand, Xiaojiang, Gao, Carrington, Mary, Miranda, Osvaldo, Castro, Carlos M., Gabriel, Martin, Drury, Patrick, Formenty, Pierre, Diallo, Boubacar, Koivogui, Lamine, Magassouba, NFaly, Carroll, Miles W., Gnther, Stephan, and Muoz-Fontela, Csar

Subjects
Ebola hemorrhagic fever -- Physiological aspects, Medical research, Immune response -- Research, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): Paula Ruibal [1, 2, 3, 4]; Lisa Oestereich [2, 3, 4]; Anja Ldtke [1, 2, 3, 4]; Beate Becker-Ziaja [2, 3, 4]; David M. Wozniak [2, 3, 4]; Romy [...]

Published
2016
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32. Clinical, virologic, and epidemiologic characteristics of dengue outbreak, Dar es Salaam, Tanzania, 2014

Author

Vairo, Francesco, Mboera, Leonard E.G., De Nardo, Pasquale, Oriyo, Ndekya M., Meschi, Silvia, Rumisha, Susan F., Colavita, Francesca, Mhina, Athanas, Carletti, Fabrizio, Mwakapeje, Elibariki, Capobianchi, Maria Rosaria, Castilletti, Concetta, Di Caro, Antonino, Nicastri, Emanuele, Malecela, Mwelecele N., and Ippolito, Giuseppe

Subjects
Biological products industry -- Health aspects, Dengue -- Health aspects, Infection -- Health aspects, Malaria -- Health aspects, Dengue viruses -- Health aspects, Health, World Health Organization
Abstract

Data are scarce on seroprevalence of dengue virus (DENV) in Tanzania. Cross-sectional studies conducted during 2007-2014 indicated that DENV seroprevalence ranged from 50%, depending on geographic area and epidemiologic characteristics [...]

Published
2016
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33. Temporal and spatial analysis of the 2014–2015 Ebola virus outbreak in West Africa

Author

Carroll, Miles W., Matthews, David A., Hiscox, Julian A., Elmore, Michael J., Pollakis, Georgios, Rambaut, Andrew, Hewson, Roger, García-Dorival, Isabel, Bore, Joseph Akoi, Koundouno, Raymond, Abdellati, Saïd, Afrough, Babak, Aiyepada, John, Akhilomen, Patience, Asogun, Danny, Atkinson, Barry, Badusche, Marlis, Bah, Amadou, Bate, Simon, Baumann, Jan, Becker, Dirk, Becker-Ziaja, Beate, Bocquin, Anne, Borremans, Benny, Bosworth, Andrew, Boettcher, Jan Peter, Cannas, Angela, Carletti, Fabrizio, Castilletti, Concetta, Clark, Simon, Colavita, Francesca, Diederich, Sandra, Donatus, Adomeh, Duraffour, Sophie, Ehichioya, Deborah, Ellerbrok, Heinz, Fernandez-Garcia, Maria Dolores, Fizet, Alexandra, Fleischmann, Erna, Gryseels, Sophie, Hermelink, Antje, Hinzmann, Julia, Hopf-Guevara, Ute, Ighodalo, Yemisi, Jameson, Lisa, Kelterbaum, Anne, Kis, Zoltan, Kloth, Stefan, Kohl, Claudia, Korva, Miša, Kraus, Annette, Kuisma, Eeva, Kurth, Andreas, Liedigk, Britta, Logue, Christopher H., Lüdtke, Anja, Maes, Piet, McCowen, James, Mély, Stéphane, Mertens, Marc, Meschi, Silvia, Meyer, Benjamin, Michel, Janine, Molkenthin, Peter, Muñoz-Fontela, César, Muth, Doreen, Newman, Edmund N. C., Ngabo, Didier, Oestereich, Lisa, Okosun, Jennifer, Olokor, Thomas, Omiunu, Racheal, Omomoh, Emmanuel, Pallasch, Elisa, Pályi, Bernadett, Portmann, Jasmine, Pottage, Thomas, Pratt, Catherine, Priesnitz, Simone, Quartu, Serena, Rappe, Julie, Repits, Johanna, Richter, Martin, Rudolf, Martin, Sachse, Andreas, Schmidt, Kristina Maria, Schudt, Gordian, Strecker, Thomas, Thom, Ruth, Thomas, Stephen, Tobin, Ekaete, Tolley, Howard, Trautner, Jochen, Vermoesen, Tine, Vitoriano, Inês, Wagner, Matthias, Wolff, Svenja, Yue, Constanze, Capobianchi, Maria Rosaria, Kretschmer, Birte, Hall, Yper, Kenny, John G., Rickett, Natasha Y., Dudas, Gytis, Coltart, Cordelia E. M., Kerber, Romy, Steer, Damien, Wright, Callum, Senyah, Francis, Keita, Sakoba, Drury, Patrick, Diallo, Boubacar, de Clerck, Hilde, Van Herp, Michel, Sprecher, Armand, Traore, Alexis, Diakite, Mandiou, Konde, Mandy Kader, Koivogui, Lamine, Magassouba, N’Faly, Avšič-Županc, Tatjana, Nitsche, Andreas, Strasser, Marc, Ippolito, Giuseppe, Becker, Stephan, Stoecker, Kilian, Gabriel, Martin, Raoul, Hervé, Di Caro, Antonino, Wölfel, Roman, Formenty, Pierre, and Günther, Stephan

Published
2015
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34. Virological and Serological Characterisation of SARS-CoV-2 Infections Diagnosed After mRNA BNT162b2 Vaccination Between December 2020 and March 2021

Author

Colavita, Francesca, primary, Meschi, Silvia, additional, Gruber, Cesare Ernesto Maria, additional, Rueca, Martina, additional, Vairo, Francesco, additional, Matusali, Giulia, additional, Lapa, Daniele, additional, Giombini, Emanuela, additional, De Carli, Gabriella, additional, Spaziante, Martina, additional, Messina, Francesco, additional, Bonfiglio, Giulia, additional, Carletti, Fabrizio, additional, Lalle, Eleonora, additional, Fabeni, Lavinia, additional, Berno, Giulia, additional, Puro, Vincenzo, additional, Bartolini, Barbara, additional, Di Caro, Antonino, additional, Ippolito, Giuseppe, additional, Capobianchi, Maria Rosaria, additional, and Castilletti, Concetta, additional

Published
2022
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35. SARS-CoV-2 Nasopharyngeal Viral Load in Individuals Infected with BA.2, Compared to Alpha, Gamma, Delta and BA.1 Variants: A Single-Center Comparative Analysis

Author

Mastrorosa, Ilaria, primary, Lepri, Alessandro Cozzi, additional, Colavita, Francesca, additional, Lalle, Eleonora, additional, Mazzotta, Valentina, additional, Cimaglia, Claudia, additional, Paulicelli, Jessica, additional, Matusali, Giulia, additional, Fabeni, Lavinia, additional, Carletti, Fabrizio, additional, Rosati, Silvia, additional, Vita, Serena, additional, Giannico, Giuseppina, additional, Piselli, Pierluca, additional, Biliotti, Elisa, additional, Al Moghazi, Samir, additional, Mosti, Silvia, additional, Girardi, Enrico, additional, Nicastri, Emanuele, additional, Garbuglia, Anna Rosa, additional, Maggi, Fabrizio, additional, Vaia, Francesco, additional, and Antinori, Andrea, additional

Published
2022
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37. Viral load decrease in SARS‐CoV‐2 BA.1 and BA.2 Omicron sublineages infection after treatment with monoclonal antibodies and direct antiviral agents.

Author

Mazzotta, Valentina, Cozzi Lepri, Alessandro, Colavita, Francesca, Rosati, Silvia, Lalle, Eleonora, Cimaglia, Claudia, Paulicelli, Jessica, Mastrorosa, Ilaria, Vita, Serena, Fabeni, Lavinia, Vergori, Alessandra, Maffongelli, Gaetano, Carletti, Fabrizio, Lanini, Simone, Caraffa, Emanuela, Milozzi, Eugenia, Libertone, Raffaella, Piselli, Pierluca, Girardi, Enrico, and Garbuglia, AnnaRosa

Subjects
ANTIVIRAL agents, MONOCLONAL antibodies, SARS-CoV-2 Omicron variant, VIRAL load, SARS-CoV-2
Abstract

The efficacy on the Omicron variant of the approved early coronavirus disease‐2019 (COVID‐19) therapies, especially monoclonal antibodies, has been challenged by in vitro neutralization data, while data on in vivo antiviral activity are lacking. We assessed potential decrease from Day 1 to Day 7 viral load (VL) in nasopharyngeal swabs of outpatients receiving Sotrovimab, Molnupiravir, Remdesivir, or Nirmatrelvir/ritonavir for mild‐to‐moderate COVID‐19 due to sublineages BA.1 or BA.2, and average treatment effect by weighted marginal linear regression models. A total of 521 patients (378 BA.1 [73%], 143 [27%] BA.2) received treatments (Sotrovimab 202, Molnupiravir 117, Nirmatrelvir/ritonavir 84, and Remdesivir 118): median age 66 years, 90% vaccinated, median time from symptoms onset 3 days. Day 1 mean VL was 4.12 log2 (4.16 for BA.1 and 4.01 for BA.2). The adjusted analysis showed that Nirmatrelvir/ritonavir significantly reduced VL compared to all the other drugs, except versus Molnupiravir in BA.2. Molnupiravir was superior to Remdesivir in both BA.1 and BA.2, and to Sotrovimab in BA.2. Sotrovimab had better activity than Remdesivir only against BA.1. Nirmatrelvir/ritonavir showed the greatest antiviral activity against Omicron variant, comparable to Molnupiravir only in the BA.2 subgroup. VL decrease could be a valuable surrogate of drug activity in the context of the high prevalence of vaccinated people and low probability of hospital admission. [ABSTRACT FROM AUTHOR]

Published
2023
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38. Virological and Serological Characterization of SARS-CoV-2 Infections Diagnosed After mRNA BNT162b2 Vaccination

Author

Colavita, Francesca, primary, Meschi, Silvia, additional, Maria Gruber, Cesare Ernesto, additional, Rueca, Martina, additional, Vairo, Francesco, additional, Matusali, Giulia, additional, Lapa, Daniele, additional, Giombini, Emanuela, additional, Carli, Gabriella De, additional, Spaziante, Martina, additional, Messina, Francesco, additional, Bonfiglio, Giulia, additional, Carletti, Fabrizio, additional, Lalle, Eleonora, additional, Fabeni, Lavinia, additional, Berno, Giulia, additional, Puro, Vincenzo, additional, Caro, Antonino Di, additional, Bartolini, Barbara, additional, Ippolito, Giuseppe, additional, Capobianchi, Maria Rosaria, additional, and Castilletti, Concetta, additional

Published
2021
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40. Molecular Characterization of Whole-Genome SARS-CoV-2 from the First Suspected Cases of the XE Variant in the Lazio Region, Italy.

Author

Rueca, Martina, Giombini, Emanuela, Gramigna, Giulia, Gruber, Cesare Ernesto Maria, Fabeni, Lavinia, Corpolongo, Angela, Mazzotta, Valentina, Corso, Luisella, Butera, Ornella, Valli, Maria Beatrice, Carletti, Fabrizio, Pignalosa, Stefano, Vairo, Francesco, Nicastri, Emanuele, Antinori, Andrea, Girardi, Enrico, Vaia, Francesco, and Maggi, Fabrizio

Subjects
SARS-CoV-2, GENETIC recombination, NUCLEOTIDE sequencing, HOSPITAL patients
Abstract

We report two cases of SARS-CoV-2 recombinant variant XE detected in nasopharyngeal swabs (NPS) of hospitalized patients with no evident epidemiological link in Lazio, Central Italy. Whole-Genome Sequencing (WGS) performed on an Ion Torrent GSS5 platform according to Italian flash surveys showed genomes corresponding to the PANGOLIN unclassified lineage and the Nextclade XE clade. Further analyses were then carried out to investigate more deeply the genetic characteristics of these XE-like sequences. When phylogenetic trees, by using IQ-TREE, were built splitting the genome into two regions according to the putative XE recombination site, the upstream and downstream regions were seen to be clustered near BA.1 and BA.2 sequences, respectively. However, our XE-like sequences clustered separately, with a significant bootstrap, from the classified European and Italian XE strains, although the recombination site between BA.1 and BA.2 was identified at the nucleotide site 11556 by RDP4 software, consistent with the putative XE breakpoint. These findings show the risk of the introduction of novel recombinant variants of SARS-CoV-2 and the existence of XE-like strains, phylogenetically separated, that could make their exact taxonomy difficult. It follows the need for continued SARS-CoV-2 surveillance by WGS. [ABSTRACT FROM AUTHOR]

Published
2022
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41. Phylogenetic analysis of West Nile virus isolates, Italy, 2008-2009

Author

Rossini, Giada, Carletti, Fabrizio, Bordi, Licia, Cavrini, Francesca, Gaibani, Paolo, Landini, Maria P., Pierro, Anna, Capobianchi, Maria R., Caro, Antonino Di, and Sambri, Vittorio

Subjects
West Nile fever -- Analysis, Infection -- Analysis, Health
Abstract

West Nile virus (WNV) is an arthropod-borne flavivirus affecting a wide range of vertebrates, including birds and mammals. The natural cycle of infection involves birds and mosquitoes (1); many species [...]

Published
2011
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42. Alkhurma hemorrhagic fever in travelers returning from Egypt, 2010

Author

Carletti, Fabrizio, Castilletti, Concetta, Di Caro, Antonino, Capobianchi, Maria R., Nisii, Carla, Suter, Fredy, Rizzi, Marco, Tebaldi, Alessandra, Goglio, Antonio, Tosi, Cristiana Passerini, and Ippolito, Giuseppe

Subjects
Hemorrhagic fever -- Health aspects, Travelers -- Health aspects, Health
Abstract

Alkhurma virus (ALKV) is a recently described member of the tick-borne hemorrhagic fever group of the genus Flavivirus. It was initially isolated in the late 1990s (1,2) and is today [...]

Published
2010
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44. Epidemiological, clinical and virological characteristics of four cases of monkeypox support transmission through sexual contact, Italy, May 2022.

Author

Antinori, Andrea, Mazzotta, Valentina, Vita, Serena, Carletti, Fabrizio, Tacconi, Danilo, Lapini, Laura Emma, D'Abramo, Alessandra, Cicalini, Stefania, Lapa, Daniele, Pittalis, Silvia, Puro, Vincenzo, Rivano Capparuccia, Marco, Giombini, Emanuela, Maria Gruber, Cesare Ernesto, Garbuglia, Anna Rosa, Marani, Alessandra, Vairo, Francesco, Girardi, Enrico, Vaia, Francesco, and Nicastri, Emanuele

Published
2022
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45. Risk and predictive factors of prolonged viral RNA shedding in upper respiratory specimens in a large cohort of COVID-19 patients admitted to an Italian reference hospital

Author

Mondi, Annalisa, primary, Lorenzini, Patrizia, additional, Castilletti, Concetta, additional, Gagliardini, Roberta, additional, Lalle, Eleonora, additional, Corpolongo, Angela, additional, Valli, Maria Beatrice, additional, Taglietti, Fabrizio, additional, Cicalini, Stefania, additional, Loiacono, Laura, additional, Di Gennaro, Francesco, additional, D’Offizi, Gianpiero, additional, Palmieri, Fabrizio, additional, Nicastri, Emanuele, additional, Agrati, Chiara, additional, Petrosillo, Nicola, additional, Ippolito, Giuseppe, additional, Vaia, Francesco, additional, Girardi, Enrico, additional, Capobianchi, Maria Rosaria, additional, Antinori, Andrea, additional, Zito, Sara, additional, Abbonizio, Maria Alessandra, additional, Abdeddaim, Amina, additional, Agostini, Elisabetta, additional, Albarello, Fabrizio, additional, Amadei, Gioia, additional, Amendola, Alessandra, additional, Antonica, Maria Assunta, additional, Antonini, Mario, additional, Bartoli, Tommaso Ascoli, additional, Baldini, Francesco, additional, Barbaro, Raffaella, additional, Bartolini, Barbara, additional, Bellagamba, Rita, additional, Benigni, Martina, additional, Bevilacqua, Nazario, additional, Biava, Gianluigi, additional, Bibas, Michele, additional, Bordi, Licia, additional, Bordoni, Veronica, additional, Boumis, Evangelo, additional, Branca, Marta, additional, Buonomo, Rosanna, additional, Busso, Donatella, additional, Camici, Marta, additional, Campioni, Paolo, additional, Canichella, Flaminia, additional, Capone, Alessandro, additional, Caporale, Cinzia, additional, Caraffa, Emanuela, additional, Caravella, Ilaria, additional, Carletti, Fabrizio, additional, Cataldo, Adriana, additional, Cerilli, Stefano, additional, Cerva, Carlotta, additional, Chiappini, Roberta, additional, Chinello, Pierangelo, additional, Cianfarani, Maria Assunta, additional, Ciaralli, Carmine, additional, Cimaglia, Claudia, additional, Cinicola, Nicola, additional, Ciotti, Veronica, additional, Colavita, Francesca, additional, Cristofaro, Massimo, additional, Curiale, Salvatore, additional, D’Abramo, Alessandra, additional, Dantimi, Cristina, additional, De Angelis, Alessia, additional, De Angelis, Giada, additional, De Palo, Maria Grazia, additional, De Zottis, Federico, additional, Di Bari, Virginia, additional, Di Lorenzo, Rachele, additional, Di Stefano, Federica, additional, Donno, Davide, additional, Evangelista, Francesca, additional, Faraglia, Francesca, additional, Farina, Anna, additional, Ferraro, Federica, additional, Fiorentini, Lorena, additional, Frustaci, Andrea, additional, Fusetti, Matteo, additional, Galati, Vincenzo, additional, Gallì, Paola, additional, Garotto, Gabriele, additional, Gaviano, Ilaria, additional, Tekle, Saba Gebremeskel, additional, Giancola, Maria Letizia, additional, Giansante, Filippo, additional, Giombini, Emanuela, additional, Granata, Guido, additional, Greci, Maria Cristina, additional, Grilli, Elisabetta, additional, Grisetti, Susanna, additional, Gualano, Gina, additional, Iacomi, Fabio, additional, Iaconi, Marta, additional, Iannicelli, Giuseppina, additional, Inversi, Carlo, additional, Lamanna, Maria Elena, additional, Lanini, Simone, additional, Lapa, Daniele, additional, Lepore, Luciana, additional, Libertone, Raffaella, additional, Lionetti, Raffaella, additional, Liuzzi, Giuseppina, additional, Lucia, Andrea, additional, Lufrani, Franco, additional, Macchione, Manuela, additional, Maffongelli, Gaetano, additional, Marani, Alessandra, additional, Marchioni, Luisa, additional, Mariano, Andrea, additional, Marini, Maria Cristina, additional, Maritti, Micaela, additional, Mastrobattista, Annelisa, additional, Mastrorosa, Ilaria, additional, Matusali, Giulia, additional, Mazzotta, Valentina, additional, Mencarini, Paola, additional, Meschi, Silvia, additional, Messina, Francesco, additional, Micarelli, Sibiana, additional, Mogavero, Giulia, additional, Mondi, Annalisa, additional, Montalbano, Marzia, additional, Montaldo, Chiara, additional, Mosti, Silvia, additional, Murachelli, Silvia, additional, Musso, Maria, additional, Nardi, Michela, additional, Navarra, Assunta, additional, Nocioni, Martina, additional, Noto, Pasquale, additional, Noto, Roberto, additional, Oliva, Alessandra, additional, Onnis, Ilaria, additional, Ottou, Sandrine, additional, Palazzolo, Claudia, additional, Pallini, Emanuele, additional, Palombi, Giulio, additional, Pareo, Carlo, additional, Passeri, Virgilio, additional, Pelliccioni, Federico, additional, Penna, Giovanna, additional, Petrecchia, Antonella, additional, Petrone, Ada, additional, Pianura, Elisa, additional, Pinnetti, Carmela, additional, Pisciotta, Maria, additional, Piselli, Pierluca, additional, Pittalis, Silvia, additional, Pontarelli, Agostina, additional, Proietti, Costanza, additional, Puro, Vincenzo, additional, Ramazzini, Paolo Migliorisi, additional, Rianda, Alessia, additional, Rinonapoli, Gabriele, additional, Rosati, Silvia, additional, Rubino, Dorotea, additional, Rueca, Martina, additional, Ruggeri, Alberto, additional, Sacchi, Alessandra, additional, Sampaolesi, Alessandro, additional, Sanasi, Francesco, additional, Santagata, Carmen, additional, Scarabello, Alessandra, additional, Scarcia, Silvana, additional, Schininà, Vincenzo, additional, Scognamiglio, Paola, additional, Scorzolini, Laura, additional, Stazi, Giulia, additional, Strano, Giacomo, additional, Taibi, Chiara, additional, Taloni, Giorgia, additional, Nardi, Tetaj, additional, Tonnarini, Roberto, additional, Topino, Simone, additional, Tozzi, Martina, additional, Vairo, Francesco, additional, Vergori, Alessandra, additional, Vincenzi, Laura, additional, Visco-Comandini, Ubaldo, additional, Vita, Serena, additional, Vittozzi, Pietro, additional, Zaccarelli, Mauro, additional, and Zanetti, Antonella, additional

Published
2021
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46. Pharmacokinetics of remdesivir and GS-441524 in two critically ill patients who recovered from COVID-19

Author

Tempestilli, Massimo, Caputi, Priscilla, Avataneo, Valeria, Notari, Stefania, Forini, Olindo, Scorzolini, Laura, Marchioni, Luisa, Ascoli Bartoli, Tommaso, Castilletti, Concetta, Lalle, Eleonora, Capobianchi, Maria R, Nicastri, Emanuele, D’Avolio, Antonio, Ippolito, Giuseppe, Agrati, Chiara, Abbonizio, Maria Alessandra, Albarello, Fabrizio, Amadei, Gioia, Amendola, Alessandra, Antinori, Andrea, Antonini, Mario, Barbaro, Raffaella, Bartolini, Barbara, Benigni, Martina, Bevilacqua, Nazario, Bordi, Licia, Bordoni, Veronica, Branca, Marta, Campioni, Paolo, Capobianchi, Maria Rosaria, Caporale, Cinzia, Caravella, Ilaria, Carletti, Fabrizio, Casetti, Rita, Chiappini, Roberta, Ciaralli, Carmine, Cimini, Eleonora, Colavita, Francesca, Corpolongo, Angela, Cristofaro, Massimo, Curiale, Salvatore, D’Abramo, Alessandra, Dantimi, Cristina, De Angelis, Alessia, De Angelis, Giada, Di Lorenzo, Rachele, Di Stefano, Federica, D’Offizi, Gianpiero, Ferraro, Federica, Fiorentini, Lorena, Frustaci, Andrea, Gallì, Paola, Garotto, Gabriele, Giancola, Maria Letizia, Giansante, Filippo, Giombini, Emanuela, Grassi, Germana, Greci, Maria Cristina, Lanini, Simone, Lapa, Daniele, Lepore, Luciana, Lucia, Andrea, Lufrani, Franco, Macchione, Manuela, Marani, Alessandra, Mariano, Andrea, Marini, Maria Cristina, Maritti, Micaela, Matusali, Giulia, Meschi, Silvia, Messina, Francesco, Montaldo, Chiara, Murachelli, Silvia, Noto, Roberto, Palazzolo, Claudia, Pallini, Emanuele, Palmieri, Fabrizio, Passeri, Virgilio, Pelliccioni, Federico, Petrecchia, Antonella, Petrone, Ada, Petrosillo, Nicola, Pianura, Elisa, Pisciotta, Maria, Pittalis, Silvia, Proietti, Costanza, Puro, Vincenzo, Rinonapoli, Gabriele, Rueca, Martina, Sacchi, Alessandra, Sanasi, Francesco, Santagata, Carmen, Scarcia, Silvana, Schininà, Vincenzo, Scognamiglio, Paola, Stazi, Giulia, Vaia, Francesco, Vairo, Francesco, and Valli, Maria Beatrice

Subjects
0301 basic medicine, Drug, Male, Microbiology (medical), medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), COVID19, media_common.quotation_subject, Critical Illness, 030106 microbiology, Pneumonia, Viral, remdesivir, Loading dose, Gastroenterology, Antiviral Agents, NO, 03 medical and health sciences, Betacoronavirus, Adenosine Triphosphate, Pharmacokinetics, Internal medicine, medicine, Humans, Pharmacology (medical), Pandemics, media_common, EC50, Original Research, Aged, Pharmacology, Lung, Alanine, Nucleoside analogue, business.industry, SARS-CoV-2, COVID-19, Recovery of Function, Prodrug, Adenosine Monophosphate, 030104 developmental biology, medicine.anatomical_structure, Infectious Diseases, Pharmacokinetics, COVID19, remdesivir, Female, business, Coronavirus Infections, medicine.drug
Abstract

Background Remdesivir is a prodrug of the nucleoside analogue GS-441524 and is under evaluation for treatment of SARS-CoV-2-infected patients. Objectives To evaluate the pharmacokinetics of remdesivir and GS-441524 in plasma, bronchoalveolar aspirate (BAS) and CSF in two critically ill COVID-19 patients. Methods Remdesivir was administered at 200 mg loading dose on the first day followed by 12 days of 100 mg in two critically ill patients. Blood samples were collected immediately after (C0) and at 1 (C1) and 24 h (C24) after intravenous administration on day 3 until day 9. BAS samples were collected on Days 4, 7 and 9 from both patients while one CSF on Day 7 was obtained in one patient. Remdesivir and GS-441524 concentrations were measured in these samples using a validated UHPLC-MS/MS method. Results We observed higher concentrations of remdesivir at C0 (6- to 7-fold higher than EC50 from in vitro studies) and a notable decay at C1. GS-441524 plasma concentrations reached a peak at C1 and persisted until the next administration. Higher concentrations of GS-441524 were observed in the patient with mild renal dysfunction. Mean BAS/plasma concentration ratios of GS-441524 were 2.3% and 6.4% in Patient 1 and Patient 2, respectively. The CSF concentration found in Patient 2 was 25.7% with respect to plasma. GS-441524 levels in lung and CNS suggest compartmental differences in drug exposure. Conclusions We report the first pharmacokinetic evaluation of remdesivir and GS-441524 in recovered COVID-19 patients. Further study of the pharmacokinetic profile of remdesivir, GS-441524 and the intracellular triphosphate form are required.

Published
2020
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47. Specialist laboratory networks as preparedness and response tool - the Emerging Viral Diseases-Expert Laboratory Network and the Chikungunya outbreak, Thailand, 2019

Author

Venturi, Giulietta, Aberle, Stephan W, Avšič-Županc, Tatjana, Barzon, Luisa, Batejat, Christoph, Burdino, Elisa, Carletti, Fabrizio, Charrel, Rémi, Christova, Iva, Connell, Jeff, Corman, Victor Max, Emmanouil, Mary, Jääskeläinen, Anne J, Kurolt, Ivan, Lustig, Yaniv, Martinez, Miguel J, Koopmans, Marion, Nagy, Orsolya, Nguyen, Trung, Papa, Anna, Pérez-Ruiz, Mercedes, Pfeffer, Martin, Protic, Jelena, Reimerink, Johan, Rossini, Giada, Sánchez-Seco Fariñas, María Paz, Schmidt-Chanasit, Jonas, Söderholm, Sandra, Sudre, Bertrand, Van Esbroeck, Marjan, and Reusken, Chantal B

Subjects
EVD-LabNet, chikungunya, lab surveillance, Thailand, lab preparedness and response, travel
Published
2020

49. Lymphofollicular lesions associated with monkeypox (Mpox) virus proctitis

Author

Mazzotta, Valentina, Scorzolini, Laura, Falasca, Laura, Lionetti, Raffaella, Aguglia, Camilla, Kontogiannis, Dimitra, Colombo, Daniele, Colavita, Francesca, De Palo, Maria Grazia, Carletti, Fabrizio, Mondi, Annalisa, Pinnetti, Carmela, Maffongelli, Gaetano, Garbuglia, Anna Rosa, Baldini, Francesco, Corpolongo, Angela, Maggi, Fabrizio, D'Offizi, Gianpiero, Girardi, Enrico, Vaia, Francesco, Nicastri, Emanuele, Del Nonno, Franca, and Antinori, Andrea

Published
2023
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50. SARS-CoV-2 Isolation From Ocular Secretions of a Patient With COVID-19 in Italy With Prolonged Viral RNA Detection

Author

Colavita, Francesca, primary, Lapa, Daniele, additional, Carletti, Fabrizio, additional, Lalle, Eleonora, additional, Bordi, Licia, additional, Marsella, Patrizia, additional, Nicastri, Emanuele, additional, Bevilacqua, Nazario, additional, Giancola, Maria Letizia, additional, Corpolongo, Angela, additional, Ippolito, Giuseppe, additional, Capobianchi, Maria Rosaria, additional, and Castilletti, Concetta, additional

Published
2020
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