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Made available in DSpace on 2022-04-29T07:25:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-01-01 This article presents the case of an 8-year-old patient who presented with a large radiolucency associated with the maxillary left canine and a supernumerary tooth. A computed tomography scan showed the radiolucency was in close proximity to the roots of the anterior teeth, with no areas of root resorption, and expansion into the left maxillary sinus. The boundaries of the maxillary sinus floor were still preserved. After positive aspiration of intralesional liquid and due to the large size of the radiolucency, a decompression technique was selected to preserve the permanent canine. Surgery was performed to remove the supernumerary tooth, followed by orthodontic treatment and surgery to allow access for orthodontic traction of the permanent canine. More than 5 years post-treatment, no recurrence was observed and the therapeutic option to position and preserve the permanent canine was successful. Department of Diagnosis and Surgery, Sao Jose Dos Campos Dental School, Sao Paulo State University Department of Oral and Maxillofacial Surgery and Traumatology, Sao Jose Dos Campos Dental School, Sao Paulo State University Department of Restorative Dentistry, Sao Jose Dos Campos Dental School, Sao Paulo State University Department of Diagnosis and Surgery, Sao Jose Dos Campos Dental School, Sao Paulo State University Department of Oral and Maxillofacial Surgery and Traumatology, Sao Jose Dos Campos Dental School, Sao Paulo State University Department of Restorative Dentistry, Sao Jose Dos Campos Dental School, Sao Paulo State University

Made available in DSpace on 2022-04-28T21:24:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-06-01 Purpose: To quantify the amount of peroxide penetration from the pulp chamber to the external surface of teeth during the walking bleaching technique. Methods: Seventy-two bovine lateral incisors were randomly divided over five experimental groups and one control (n= 12 per group): (1) 35% hydrogen peroxide (HP); (2) 35% carbamide peroxide (CP); (3) sodium perborate (SP); (4) (HP+SP); (5) (CP+SP) and (6) Control (CG), deionized water. All groups were treated according to the walking bleach technique. After 7 days at 37°C in an acetate buffer solution, 100 μl violet leukocrystal coloring and 50 μl peroxidase was added, producing a blue stain that could be measured in a spectrophotometer and then converted into peroxide μg/ml. Results: G5 exhibited the greatest penetration, while G2 and G3 produced the lowest values. All bleaching agents penetrated from the pulp chamber to the external root surface. There was a direct correlation between the presence of oxidative agents and penetration potential. Sodium perborate in distilled water was less oxidative and appeared to be the least aggressive bleaching agent. Department of Endodontics São Paulo State University, Araraquara, SP Department of Biochemistry São Paulo State University, São José dos Campos, SP University of Connecticut, Farmington, CT Department of Endodontics São Paulo State University, Araraquara, SP Department of Biochemistry São Paulo State University, São José dos Campos, SP

Made available in DSpace on 2014-06-11T19:31:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-08-26Bitstream added on 2014-06-13T20:22:21Z : No. of bitstreams: 1 cardoso_pe_dr_sjc.pdf: 421203 bytes, checksum: 78d99972a840d87b24c7feb43f8fd9db (MD5) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) A proposta deste estudo foi avaliar a citotoxicidade dos peróxidos de hidrogênio (PH) e carbamida (PC), para uso caseiro e profissional, sobre cultura de fibroblastos de mucosa de tecido gengival humano (FMM1). As células utilizadas foram cultivadas em DMEM e após 24 horas foi colocado meio de cultivo condicionado com os agentes clareadores: G1- PC 35% sem fotoativação (SF); G2- PC 35% ativado por luz halógena; G3- PC 35% ativado por diodo emissor de luz (LED); G4- PC 37% SF; G5- PC 37% ativado por luz halógena; G6- PC 37% ativado por LED; G7- PH 3% SF; G8- PH 7,5% SF; G9- PH 9,5% SF; G10- PC 10% SF; G11- PC 15% SF e G12- PC 20% SF. Uma curva padrão de viabilidade celular foi obtida a partir de células que não receberam tratamento (controle). O ensaio com MTT foi realizado após 24 e 48 horas, para avaliar a viabilidade celular. Paralelamente, foi medido colorimetricamente a quantidade de PH liberado nas condições experimentais. Os dados da viabilidade celular obtidos foram analisados estatisticamente através dos testes ANOVA e Tukey, (p

Made available in DSpace on 2022-04-28T21:00:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-01-01 Purpose: The aim of this study was to quantitatively evaluate and qualitatively describe autogenous bone graft healing with or without an expanded polytetrafluoroethylene (e-PTFE) membrane in ovariectomized rats. Materials and Methods: Eighty Wistar rats, weighing approximately 300 g each, were used. A graft was obtained from the parietal bone and fixed to the sidewall of each animal’s left mandibular ramus. The animals were randomly divided into four experimental groups (n = 20 in each group): group 1, sham operated and autogenous bone graft only; group 2, sham operated and autogenous bone graft covered by e-PTFE membrane; group 3, ovariectomized (OVX) and autogenous bone graft only; group 4, OVX and autogenous bone graft covered by e-PTFE membrane. The animals were sacrificed at five different time points: immediately after grafting or at 7, 21, 45, or 60 days after grafting. Histologic examination and morphometric measurement of the sections were performed, and values were submitted to statistical analyses. Results: Both groups (sham and OVX) experienced loss of the original graft volume when it was not covered by the membrane, whereas use of the membrane resulted in additional bone formation beyond the edges of the graft and under the membrane. Histologic analysis showed integration of the grafts in all animals, although a larger number of marrow spaces was found in OVX groups. Conclusions: Association of bone graft with an e-PTFE membrane resulted in maintenance of its original volume as well as formation of new bone that filled the space under the membrane. Osteopenia did not influence bone graft repair, regardless of whether or not it was associated with e-PTFE membrane, but descriptive histologic analysis showed larger numbers of marrow spaces in the bone graft and receptor bed and formation of new bone in the OVX animals. © 2009 by Quintessence Publishing Co Inc. Department of Diagnosis and Surgery São Paulo State University (UNESP - São José dos Campos) School of Dentistry Ibirapuera University, SP Department of Restorative Dentistry São Paulo State University (UNESP - São José dos Campos) School of Dentistry Department of Stomatology University of São Paulo, São Paulo Department of Diagnosis and Surgery São Paulo State University (UNESP - São José dos Campos) School of Dentistry Department of Restorative Dentistry São Paulo State University (UNESP - São José dos Campos) School of Dentistry

Made available in DSpace on 2022-04-28T20:55:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-01-01 This aim of the present study was to evaluate the pulp chamber penetration of 35% hydrogen peroxide activated by LED (light-emitting diode) or Nd:YAG laser in bovine teeth, after an in-office bleaching technique. Forty-eight bovine lateral incisors were divided into four groups, acetate buffer was placed into the pulp chamber and bleaching agent was applied as follows: for group A (n = 12), activation was performed by LED; for group B (n = 12), activation was performed by Nd:YAG laser (60 mJ, 20 Hz); group C (n = 12) received no light or laser activation; and the control group (n = 12) received no bleaching gel application or light or laser activation. The acetate buffer solution was transferred to a glass tube and Leuco Crystal Violet and horseradish peroxidase were added, producing a blue solution. The optical density of this solution was determined spectrophotometrically and converted into microgram equivalents of hydrogen peroxide. The results were analysed using ANOVA and Tukey's test (5%). It was verified that the effect of activation was significant, as groups activated by LED or laser presented greater hydrogen peroxide penetration into the pulp chamber (0.499 +/- 0.622 microg) compared with groups that were not (0.198 +/- 0.218 microg). There was no statistically significant difference in the penetration of hydrogen peroxide into the pulp chamber between the two types of activation (LED or laser). The results suggest that activation by laser or LED caused an increase in hydrogen peroxide penetration into the pulp chamber. School of Dentistry Department of Oral Pathology São Paulo State University - UNESP São José dos Campos School of Dentistry Department of Oral Pathology São Paulo State University - UNESP São José dos Campos