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4. Potential Prebiotic Effect of Inulin-Enriched Pasta after In Vitro Gastrointestinal Digestion and Simulated Gut Fermentation

Author

Bavaro, Anna Rita, primary, Di Biase, Mariaelena, additional, Linsalata, Vito, additional, D’Antuono, Isabella, additional, Di Stefano, Vita, additional, Lonigro, Stella Lisa, additional, Garbetta, Antonella, additional, Valerio, Francesca, additional, Melilli, Maria Grazia, additional, and Cardinali, Angela, additional

Published
2024
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5. Spotlight on Secondary Metabolites Produced by an Early-Flowering Apulian Artichoke Ecotype Sanitized from Virus Infection by Meristem-Tip-Culture and Thermotherapy.

Author

Spanò, Roberta, Gena, Patrizia, Linsalata, Vito, Sini, Valeria, D'Antuono, Isabella, Cardinali, Angela, Cotugno, Pietro, Calamita, Giuseppe, and Mascia, Tiziana

Subjects
SUPERCRITICAL fluid extraction, CARDOON, REACTIVE oxygen species, VIRUS diseases, METABOLITES, POLYPHENOLS, CHLOROGENIC acid
Abstract

Globe artichoke (Cynara cardunculus L. subsp. scolymus) is an important crop of the Mediterranean basin characterized by many properties, like hepatoprotective, anticarcinogenic, antioxidant, antibacterial, and beneficial to human health. The high bioactive compounds (BACs) content, as polyphenols, has attracted the research interest in artichoke extracts. We analysed the changes in polyphenol transcriptome profile between sanitized (S) virus-free and non-sanitized (NS) artichoke plants, focusing on genes involved in phenylpropanoid metabolic pathway and flavonoid biosynthesis. A total of 2458 upregulated and 2154 downregulated differentially expressed genes (DEGs) were functionally characterized. Among them, 31 and 35 KEGG orthology entries characterized by upregulated and downregulated DEGs, respectively, were involved in the biosynthesis of other secondary metabolites. A downregulation of PAL, C4H, 4CL, HST/HQT, C3′H, CCoAMT, CCR1, and F5H, was observed in S artichoke compared to NS one, whereas the CSE, CHS, and CHI genes were upregulated in S samples. Transcriptome results were compared to the polyphenols accumulation in S and NS artichoke leaves. A higher content of total polyphenols was observed in older leaves of NS samples, compared to extracts obtained from young leaves or from S plants, and this result was associated with the presence of viral infections in NS plants. In all the conditions tested, the most represented compound was chlorogenic acid, followed by luteolin-7-O-glucoside. The different composition of each extract was evaluated by a polyphenol dose–response treatment on the rodent hepatoma FaO cell line to the accumulation of reactive oxygen species (ROS). A significant reduction in ROS content ranging between −40% and −48% was observed when 10–20 mg/L of polyphenols from NS or S plants were used, characterized by a specific profile of compounds. To reduce MetOH residues in polyphenol extracts, a supercritical fluid CO2 extraction was evaluated to propose a sustainable green extraction. [ABSTRACT FROM AUTHOR]

Published
2024
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19. Innovative Use of Olive, Winery and Cheese Waste By-Products as Functional Ingredients in Broiler Nutrition

Author

Bonos, Eleftherios, primary, Skoufos, Ioannis, additional, Petrotos, Konstantinos, additional, Giavasis, Ioannis, additional, Mitsagga, Chrysanthi, additional, Fotou, Konstantina, additional, Vasilopoulou, Konstantina, additional, Giannenas, Ilias, additional, Gouva, Evangelia, additional, Tsinas, Anastasios, additional, D’Alessandro, Angela Gabriella, additional, Cardinali, Angela, additional, and Tzora, Athina, additional

Published
2022
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22. INNOVATIVE USE OF OLIVE, WINERY AND CHEESE WASTE BY PRODUCTS IN ANIMAL NUTRITION FOR THE PRODUCTION OF FUNCTIONAL FOODS FROM ANIMALS INNOTRITION 6 Progress Report PB4

Author

Cardinali Angela, Isabella D'Antuono, Federico Baruzzi, Antonella Garbetta, Vito Linsalata, and Mariella Quarto

Subjects
cheese whey, Silage, Functional foods, Grape Pomace, Polyphenols, Olive mill waste water
Abstract

6 report del progetto INNOTRITION relativo al periodo tra 1 Luglio 2021 sino a 31 Dicembre 2021

Published
2021

23. INNOVATIVE USE OF OLIVE, WINERY AND CHEESE WASTE BY PRODUCTS IN ANIMAL NUTRITION FOR THE PRODUCTION OF FUNCTIONAL FOODS FROM ANIMALS INNOTRITION 5 Progress Report PB4

Author

Cardinali Angela, Isabella D'Antuono, Federico Baruzzi, Antonella Garbetta, Vito Linsalata, and Mariella Quarto

Subjects
cheese whey, Silage, Functional foods, Grape Pomace, Polyphenols, Olive mill waste water
Abstract

5 report del progetto INNOTRITION relativo al periodo tra 1 Gennaio 2021 sino a 30 giugno 2021

Published
2021

25. INNOVATIVE USE OF OLIVE, WINERY AND CHEESE WASTE BY PRODUCTS IN ANIMAL NUTRITION FOR THE PRODUCTION OF FUNCTIONAL FOODS FROM ANIMALS INNOTRITION 3 Progress Report PB4

Author

Cardinali Angela, Isabella D'Antuono, Federico Baruzzi, Antonella Garbetta, Vito Linsalata, and Mariella Quarto

Subjects
cheese whey, Silage, Functional foods, Grape Pomace, Polyphenols, Olive mill waste water
Abstract

3 report del progetto INNOTRITION relativo al periodo tra 1 Gennaio 2020 sino a 30 giugno 2020.

Published
2020

26. INNOVATIVE USE OF OLIVE, WINERY AND CHEESE WASTE BY PRODUCTS IN ANIMAL NUTRITION FOR THE PRODUCTION OF FUNCTIONAL FOODS FROM ANIMALS INNOTRITION 4 Progress Report PB4

Author

Cardinali Angela, Isabella D'Antuono, Federico Baruzzi, Antonella Garbetta, Vito Linsalata, and Mariella Quarto

Subjects
cheese whey, Silage, Functional foods, Grape Pomace, Polyphenols, Olive mill waste water
Abstract

4 report del progetto INNOTRITION relativo al periodo tra 1 Luglio 2020 sino a 31 gennaio 2020.

Published
2020

29. Report scientifico Joint Lab 'Functional Lab' 3 anno

Author

Cardinali Angela, D'Antuono Isabella, and Maria Quarto

Subjects
Pasta funzionale, Inulina, Opuntia, Digestione in vitro, Olive da Tavola, Polifenoli
Abstract

L'attività di ricerca condotta durante il III ed ultimo anno del progetto Functional Lab ha riguardato l'avvio di alcune nuove attività di ricerca ed il completamento di alcune sperimentazioni in collaborazione con il partner straniero. In particolare, è stata studiata la componente bioattiva in cladodi di Opuntia Ficus indica allo scopo di valorizzare questo sottoprodotto per possibili ricadute salutistiche. Lo studio è stato realizzato in collaborazione di un istituto di ricerca dell'Università di Al Lamar Tunisia. Si è inoltre avviato lo studio su di una pasta arricchita con inulina a differente grado di polimerizzazione, allo scopo di evidenziare come tale parametro possa influire sull'effetto prebiotico della stessa. E' stato approvato dall'Istituto Superiore di Sanità il progetto di sperimentazione in vivo su animali da laboratorio (Autorizzazione n° 849/2019-PR. Risp. a prot. BC793.26), per valutare l'influenza della componente fenolica caratteristica della cultivar di olive Cellina di Nardò, sulla riduzione e prevenzione della steatosi epatica non alcolica in topi obesi. Il progetto sarà svolto in collaborazione con l'Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) "De Bellis" di Castellana Grotte (BA). La Dr.ssa Isabella D'Antuono ricercatrice dell'CNR-ISPA ha trascorso 2 mesi (maggio-luglio 2019) presso l'Università Laval Quebéc, Canada per studiare come viene modulato il microbiota umano da polifenoli della filiera dell'olio di oliva. Interessante opportunità derivata dalla collaborazione con i partner Canadesi dell'Università Laval, ha riguardato il coinvolgimento dei ricercatori del CNR-ISPA, nel progetto Joint International Research Unit between the CNR and Université Laval for Chemical and Biochemical Research on the Microbiome and its Impact on Metabolic Health and Nutrition (UMI-MicroMeNu). Nell'ambito dell'UMI-MicroMeNu sono stati avviati 8 progetti satellite dei quali, il progetto Targeting the gut microbiota with new extracts enriched with omega-3 PUFAs and polyphenols from nordic biosources to alleviate cardiometabolic diseases, vede la partecipazione della Dr.ssa Cardinali Angela (CNR- ISPA, Italia) insieme al Prof André Marette dell'Università Laval Quebéc, Canada, ed al Prof. Sebastiano Banni dell'Università di Cagliari, Italia.

Published
2019

30. INNOVATIVE USE OF OLIVE, WINERY AND CHEESE WASTE BY PRODUCTS IN ANIMAL NUTRITION FOR THE PRODUCTION OF FUNCTIONAL FOODS FROM ANIMALS INNOTRITION 2 Progress Report PB4

Author

Cardinali Angela, Isabella D'Antuono, Federico Baruzzi, Antonella Garbetta, Vito Linsalata, and Mariella Quarto

Subjects
cheese whey, Silage, olive mill waste water, Functional foods, Grape Pomace, Polyphenols, Animal feed
Abstract

2 report del progetto INNOTRITION relativo al periodo tra 1 Ottobre 2019 sino a 31 Dicembre 2019. Durante questo periodo si è anche tenuto il 1 meeting di progetto al quale hanno partecipato tutti i Partners. Tale informazione è anche visibile nel sito web ISPA al link: https://www.ispacnr.it/progetto-inno-trition-comunicato-stampa/

Published
2019

31. INNOVATIVE USE OF OLIVE, WINERY AND CHEESE WASTE BY PRODUCTS IN ANIMAL NUTRITION FOR THE PRODUCTION OF FUNCTIONAL FOODS FROM ANIMALS INNOTRITION 1 Progress Report

Author

Cardinali Angela, Isabella D'Antuono, Federico Baruzzi, Antonella Garbetta, Vito Linsalata, and Mariella Quarto

Subjects
cheese whey, Silage, Functional foods, Grape Pomace, Polyphenols, Olive mill waste water, Animal feed
Abstract

I Project Report describing the project implementation for PB4 from the April 1th 2019 to September 30th 2019

Published
2019

32. An Integrated Caco-2TC7cells/biosensors Device for the Real Time Monitoring of Intestinal Glucose and Polyphenols Absorption and Hypoglycemic Effect of Phytochemicals

Author

D’hallewin Guy, D’Antuono Isabella, Garbetta Antonella, Barberis Antonio, Bazzu Gianfranco, Minervini Fiorenza, Rocchitta Gaia, Linsalata Vito, Cardinali Angela, Fadda Angela, and Serra Pier Andrea

Subjects
0301 basic medicine, Laccase, Absorption (pharmacology), biology, Chemistry, Tyrosinase, 010401 analytical chemistry, technology, industry, and agriculture, macromolecular substances, 01 natural sciences, 0104 chemical sciences, 03 medical and health sciences, 030104 developmental biology, Postprandial, Biochemistry, Cell culture, Polyphenol, biology.protein, General Earth and Planetary Sciences, Glucose oxidase, Biosensor, General Environmental Science
Abstract

An integrated device, for real-time monitoring of glucose and phenols absorption, that consists of a sensors/biosensors system (SB) and a Caco-2TC7 human intestinal cell culture, is shown here. The SB was made of a glucose oxidase-based biosensor, a sentinel platinum sensor, a laccase/tyrosinase-based biosensor and a sentinel carbon sensor located in the basolateral compartment (BC) of a cell culture plate. This system was able to monitor the glucose absorption and the hypoglycemic effect induced by different polyphenols and could be proposed to provide an effective strategy to manage postprandial hyperglycemia with natural compounds.

Published
2017

33. Altered morphokinetics in equine embryos from oocytes exposed to DEHP during IVM

Author

Marzano, Giuseppina, primary, Mastrorocco, Antonella, additional, Zianni, Rosalia, additional, Mangiacotti, Michele, additional, Chiaravalle, Antonio E., additional, Lacalandra, Giovanni M., additional, Minervini, Fiorenza, additional, Cardinali, Angela, additional, Macciocca, Maria, additional, Vicenti, Rossella, additional, Fabbri, Raffaella, additional, Hinrichs, Katrin, additional, Dell’Aquila, Maria E., additional, and Martino, Nicola A., additional

Published
2019
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35. In vitro activity of antimicrobial compounds against X. fastidiosa causing OQDS in Apulia (IT)

Author

Bleve Gianluca, Altomare Claudio Fabio, Cardinali Angela, Vurro Maurizio, Gallo Antonia, Logrieco Antonio Francesco, Marchi Guido, and Mita Giovanni

Subjects
microbial metabolites, antibiotics, phenolics, olive mill wastewater, fungal extracts, toxins
Abstract

Olive quick decline syndrome (OQDS) caused by X. fastidiosa is currently causing severe damages to the production and reducing the life span of the plants in the Salento peninsula of Apulia (Italy). No effective means of control of X. fastidiosa is currently available. The objective of this study was to evaluate in vitro antimicrobial activities against X. fastidiosa (strain Salento-1) of different classes of compounds having diverse origins, i.e. traditional antibiotics, plant-derived natural products, and microbial metabolites. A preliminary bioassay, performed by the agar disc diffusion method, revealed that 17 of the 31 antibiotics tested did not affect bacterial growth at a dose of 5 ?g. Olive mill wastewaters (OMWs), which are known to possess a broad range of antimicrobial activity, are able to inhibit X. fastidiosa in vitro. Most interestingly when we analysed different OMWs derived micro, ultra and nano-filtered fractions as well as some of the single phenolic compounds that they contain, we found that the OMWs micro-filtered fraction is the most effective against the bacterium but only few phenolics are active in their pure form. Also some fungal extracts and bacteria toxins showed noteworthy inhibitory effect to strain Salento-1 growth. The possible use of some of these products for curative/preventive treating OQDS-affected or at-risk olive plants will be discussed.

Published
2017

36. Biological Activity of High Molecular Weight Phenolics from Olive Mill Wastewater

Author

Cardinali, Angela, Cicco, Nunzia, Linsalata, Vito, Minervini, Fiorenza, Pati, Sandra, Pieralice, Maria, Tursi, Nunzia, and Lattanzio, Vincenzo

Abstract

Olive oil production generates large amounts of recalcitrant compounds, the olive oil mill wastewater (OMWW), which represent one of the most contaminating effluents among those produced by the agrofood industries. Nowadays, this view has changed to one that recognizes the waste as a low-cost starting material rich in bioactive compounds, particularly biophenols, that can be extracted and applied as natural antioxidants for the food and pharmaceutical industries. The data reported in this paper indicate that the OMWW extracts, besides low molecular weight antioxidant phenolics such as tyrosol and hydroxytyrosol, also contain phenolics with a molecular weight in the range of 600−5000 Da, which exhibit efficient scavenging activities against hydroxyl and peroxyl radicals. This group of phenolics includes, besides verbascoside, isoverbascoside, and an oxidized form of verbascoside, a number of higher molecular weight phenolics arising from oxidative polymerization of hydroxytyrosol and caffeic acid. Overall, these higher molecular weight phenolics prove to be, in some in vitro tests, more efficient scavengers of hydrophilic hydroxyl radicals than hydroxytyrosol, which could be used for industrial applications as natural nontoxic antioxidants.

Published
2024
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37. Use of Olive Oil Industrial By-Product for Pasta Enrichment

Author

Padalino, Lucia, primary, D’Antuono, Isabella, additional, Durante, Miriana, additional, Conte, Amalia, additional, Cardinali, Angela, additional, Linsalata, Vito, additional, Mita, Giovanni, additional, Logrieco, Antonio, additional, and Del Nobile, Matteo, additional

Published
2018
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39. Rapporto tecnico Finale PROINNOBIT: Sviluppo di prodotti alimentari innovativi mediante soluzioni biotecnologiche, impiantistiche e tecnologiche

Author

Boari Francesca, Cardinali Angela, Caputo Leonardo, Baruzzi Federico, Giuseppe Cozzi, Linsalata Vito, Minervini Fiorenza, D'Antuono Isabella, Garbetta Antonella, Logrieco Antonio, and Martena Simonetta

Subjects
Shelf life, Uva da tavola, Termopallet, Polifenoli, Refrigerazione Passiva, Ozono
Abstract

Rapporto tecnico Finale progetto PROINNOBIT

Published
2016

40. Relazione scientifica CISIA FINALE 2014- 2016

Author

Cardinali Angela, Minervini Fiorenza, D'Antuono Isabella, Garbetta Antonella, and Lavermicocca Paola

Subjects
antinfiammatorio, digestione in vitro, carciofo probiotico, antiossidante, carciofo
Abstract

Relazione scientifica finale Progetto CISIA

Published
2016

43. VII SAL Progetto PROINNOBIT

Author

Baruzzi Federico, Boari Francesca, Caputo Leonardo, Cardinali Angela, Cozzi Giuseppe, D'Antuono Isabella, Garbetta Antonella, Linsalata Vito, Logrieco Antonio F., and Minervini Fiorenza Martena Simonetta .

Subjects
Uva da tavola, Termopallet, Refrigerazione Passiva, Polifenoli, Ozono
Abstract

RAPPORTO TECNICO DELLO STATO D'AVANZAMENTO INTERMEDIO DEL PROGETTO DI RICERCA Titolo del progetto: PROINNOBIT: Sviluppo di prodotti alimentari innovativi mediante soluzioni biotecnologiche, impiantistiche e tecnologiche Codice Progetto: PON02_00186_3401737 Stato di avanzamento n. 7 dal 01/01/2015 al 30/06/2015

Published
2015

44. VIII SAL PROINNOBIT: Sviluppo di prodotti alimentari innovativi mediante soluzioni biotecnologiche, impiantistiche e tecnologiche

Author

Baruzzi Federico, Boari Francesca, Caputo Leonardo, Cardinali Angela, Cozzi Giuseppe, D'Antuono Isabella, Garbetta Antonella, Linsalata Vito, Logrieco Antonio F., Minervini Fiorenza, and Martena Simonetta.

Subjects
Refrigerazione passiva, Uva da tavola, Termopallet, Polifenoli, Ozono
Abstract

Stato di avanzamento n. 8 Progetto PROINNOBIT:Sviluppo di prodotti alimentari innovativi mediante soluzioni biotecnologiche, impiantistiche e tecnologiche

Published
2015

45. Identification of biological parameters for monitoring the quality of olive oil mill waste waters after purification and recovery of bioactive compounds

Author

Giorgetti Lucia, Longo Vincenzo, Cardinali Angela, D'Antuono Isabella, Linsalata Vito, Nazzaro Filomena, Fratianni Florinda, and Coppola Raffaele

Subjects
OMWW, antibiotic activity, Phytotoxicity, genotoxicity, food and beverages
Abstract

Olive oil mill waste waters (OMWWs), a polluting byproduct of the olive oil production process, contain large amount of organics and inorganics compounds (polyphenols, salts, etc) that, if inappropriately discharged, can cause environmental pollution. Polyphenols have several biological activity, but at high concentration they are toxic and their removal from OMWW is an important matter. In this work the detoxification of OMWW, performed by membrane filtration systems, was investigated with the dual aims, of reducing the organic load of OMWW and recovering polyphenols. The OMWWs were processed by ceramic and polymeric membranes with different porosity, to obtain three fractions: microfiltrate (MF, 100,000 Da), ultrafiltrate (UF, 5000 Da) and nanofiltrate (NF, 200 Da). The fractions were characterized by HPLC analysis, to quantify the main polyphenols present: Hydroxytyrosol, Tyrosol, Caffeic and Coumaric Acids, Verbascoside, Isoverbascoside, Secologanoside, and Comselogoside. MF and UF fractions exhibited antimicrobial activity against Escherichia coli, as well as against Pseudomonas aeruguinosa and Staphylococcus aureus pathogen strains. No activity was given by NF fraction. Thus, MF and UF might be considered as natural antibiotics, taking into account mainly their effect in inhibiting the growth of E. coli and S. aureus. Phytotoxic and genotoxic effects were analysed in Vicia faba L. plants germinated in MF, UF and NF fractions. The major phytotoxic effects were shown in seeds germinated in MF fraction. Genotoxicity analysis relied on mitotic activity (MI), aberration index (AI) and micronucleus frequency (MCN): MI was generally reduced, cytogenetic aberrations were found in all the treatments and MCN were mostly present in MF fraction. Results generally indicated the efficiency in polyphenols recovery and natural antibiotic activity of MF and UF fractions. Genotoxicity and phytotoxicity effects of filtrated OMWWs suggested the need of further treatments/ dilutions before their discharge in the environment.

Published
2015

48. V SAL PROINNO-BIT: Sviluppo di prodotti alimentari innovativi mediante soluzioni biotecnologiche, impiantistiche e tecnologiche

Author

Boari Francesca, Cardinali Angela, Caputo Leonardo, Baruzzi Federico, Giuseppe Cozzi, Linsalata Vito, Minervini Fiorenza, D'Antuono Isabella, Garbetta Antonella, Logrieco Antonio, Martena Simonetta, and Lattanzio Vincenzo

Subjects
Refrigerazione passiva, Uva da Tavola, Termopallet, Polifenoli
Abstract

Stato di avanzamento n. 5 dal 1/01/2014 al 30/06/2014 (Proroga attività progettuali dal 1/11/2014 al 30/06/2015). Le attività previste sono state condotte nei termini previsti dal progetto e non ci sono stati scostamenti nell'esecuzione delle stesse.

Published
2014

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