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26 results on '"Carcione, Claudia"'

3. Caspase-8 activation by cigarette smoke induces pro-inflammatory cell death of human macrophages exposed to lipopolysaccharide

Author

Cristaldi, Marta, Buscetta, Marco, Cimino, Maura, La Mensa, Agnese, Giuffrè, Maria Rita, Fiore, Luigi, Carcione, Claudia, Bucchieri, Fabio, Rappa, Francesca, Coronnello, Claudia, Sciaraffa, Nicolina, Amato, Santina, Aronica, Tommaso Silvano, Lo Iacono, Giovanna, Bertani, Alessandro, Pace, Elisabetta, and Cipollina, Chiara

Published
2023
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4. Human Amniotic MSC Response in LPS-Stimulated Ascites from Patients with Cirrhosis: FOXO1 Gene and Th17 Activation in Enhanced Antibacterial Activation

Author

Pampalone, Mariangela, primary, Cuscino, Nicola, additional, Iannolo, Gioacchin, additional, Amico, Giandomenico, additional, Ricordi, Camillo, additional, Vitale, Giampiero, additional, Carcione, Claudia, additional, Castelbuono, Salvatore, additional, Scilabra, Simone Dario, additional, Coronnello, Claudia, additional, Gruttadauria, Salvatore, additional, and Pietrosi, Giada, additional

Published
2024
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5. Proteomic analysis and functional validation reveal distinct therapeutic capabilities related to priming of mesenchymal stromal/stem cells with IFN-γ and hypoxia: potential implications for their clinical use.

Author

Calligaris, Matteo, Zito, Giovanni, Busà, Rosalia, Bulati, Matteo, Iannolo, Gioacchin, Gallo, Alessia, Carreca, Anna Paola, Cuscino, Nicola, Castelbuono, Salvatore, Carcione, Claudia, Centi, Claudio, Amico, Giandomenico, Bertani, Alessandro, Chinnici, Cinzia Maria, Conaldi, Pier Giulio, Scilabra, Simone Dario, and Miceli, Vitale

Subjects
STEM cells, FUNCTIONAL analysis, PROTEOMICS, HYPOXEMIA, AMNION, CARCINOSARCOMAS, CEREBRAL anoxia-ischemia
Abstract

Mesenchymal stromal/stem cells (MSCs) are a heterogeneous population of multipotent cells that can be obtained from various tissues, such as dental pulp, adipose tissue, bone marrow and placenta. MSCs have gained importance in the field of regenerative medicine because of their promising role in cell therapy and their regulatory abilities in tissue repair and regeneration. However, a better characterization of these cells and their products is necessary to further potentiate their clinical application. In this study, we used unbiased high-resolution mass spectrometry-based proteomic analysis to investigate the impact of distinct priming strategies, such as hypoxia and IFN-γ treatment, on the composition and therapeutic functionality of the secretome produced by MSCs derived from the amniotic membrane of the human placenta (hAMSCs). Our investigation revealed that both types of priming improved the therapeutic efficacy of hAMSCs, and these improvements were related to the secretion of functional factors present in the conditioned medium (CM) and exosomes (EXOs), which play crucial roles in mediating the paracrine effects of MSCs. In particular, hypoxia was able to induce a pro-angiogenic, innate immune responseactivating, and tissue-regenerative hAMSC phenotype, as highlighted by the elevated production of regulatory factors such as VEGFA, PDGFRB, ANGPTL4, ENG, GRO-γ, IL8, and GRO-α. IFN-γ priming, instead, led to an immunosuppressive profile in hAMSCs, as indicated by increased levels of TGFB1, ANXA1, THBS1, HOMER2, GRN, TOLLIP and MCP-1. Functional assays validated the increased angiogenic properties of hypoxic hAMSCs and the enhanced immunosuppressive activity of IFN-γ-treated hAMSCs. This study extends beyond the direct priming effects on hAMSCs, demonstrating that hypoxia and IFN-γ can influence the functional characteristics of hAMSC-derived secretomes, which, in turn, orchestrate the production of functional factors by peripheral blood cells. This research provides valuable insights into the optimization of MSC-based therapies by systematically assessing and comparing the priming type-specific functional features of hAMSCs. These findings highlight new strategies for enhancing the therapeutic efficacy of MSCs, particularly in the context of multifactorial diseases, paving the way for the use of hAMSC-derived products in clinical practice. [ABSTRACT FROM AUTHOR]

Published
2024
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6. 3D Culture and Interferon-γ Priming Modulates Characteristics of Mesenchymal Stromal/Stem Cells by Modifying the Expression of Both Intracellular and Exosomal microRNAs

Author

Bulati, Matteo, primary, Gallo, Alessia, additional, Zito, Giovanni, additional, Busà, Rosalia, additional, Iannolo, Gioacchin, additional, Cuscino, Nicola, additional, Castelbuono, Salvatore, additional, Carcione, Claudia, additional, Centi, Claudio, additional, Martucci, Gennaro, additional, Bertani, Alessandro, additional, Baiamonte, Maria Pia, additional, Chinnici, Cinzia Maria, additional, Conaldi, Pier Giulio, additional, and Miceli, Vitale, additional

Published
2023
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8. Oncolytic Effect of Zika Virus in Neuroendocrine Pancreatic Tumors: New Perspectives for Therapeutic Approaches.

Author

Cocco, Martina Maria, Carcione, Claudia, Miceli, Vitale, Tinnirello, Rosaria, Chinnici, Cinzia Maria, Carbone, Carmine, Zito, Giovanni, Conaldi, Pier Giulio, and Iannolo, Gioacchin

Subjects
*PANCREATIC tumors, *NEUROENDOCRINE tumors, *ZIKA virus, *CELL death inhibition, *PANCREATIC cancer
Abstract

Pancreatic cancer (PCa) is the fifth leading cause of cancer mortality. Recently, our group and others have demonstrated the oncolytic activity of the Zika virus (ZIKV) against glioblastoma. The peculiar features of this virus offer the opportunity to use an agent already tested in vivo through natural transmission, with minimal effects on adults, to specifically target a tumor such as glioblastoma. This remarkable specificity prompted us to explore the potential use of ZIKV oncolytic action against other tumor types. In particular, we focused on the subgroup of pancreatic tumors with a neuroendocrine origin known as neuroendocrine tumors (NETs). We found that ZIKV exerts its oncolytic activity by specifically infecting NET cells, leading to growth inhibition and cell death. We also assessed whether the oncolytic action could be extended to pancreatic tumors different from NETs. However, as expected, the viral specificity is limited to NETs and is not applicable to adenocarcinoma tumors, indicating a narrow spectrum of action for this virus. These findings support the potential use of ZIKV in therapeutic approaches not only in glioblastoma, but also against other tumors, such as neuroendocrine pancreatic tumors. [ABSTRACT FROM AUTHOR]

Published
2023
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11. Galectin-9 and Interferon-Gamma Are Released by Natural Killer Cells upon Activation with Interferon-Alpha and Orchestrate the Suppression of Hepatitis C Virus Infection

Author

Carreca, Anna Paola, primary, Gaetani, Massimiliano, additional, Busà, Rosalia, additional, Francipane, Maria Giovanna, additional, Gulotta, Maria Rita, additional, Perricone, Ugo, additional, Iannolo, Gioacchin, additional, Russelli, Giovanna, additional, Carcione, Claudia, additional, Conaldi, Pier Giulio, additional, and Badami, Ester, additional

Published
2022
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12. Human Amnion-Derived Mesenchymal Stromal/Stem Cells Pre-Conditioning Inhibits Inflammation and Apoptosis of Immune and Parenchymal Cells in an In Vitro Model of Liver Ischemia/Reperfusion

Author

Zito, Giovanni, primary, Miceli, Vitale, additional, Carcione, Claudia, additional, Busà, Rosalia, additional, Bulati, Matteo, additional, Gallo, Alessia, additional, Iannolo, Gioacchin, additional, Pagano, Duilio, additional, and Conaldi, Pier Giulio, additional

Published
2022
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14. Changes in the Transcriptome Profiles of Human Amnion-Derived Mesenchymal Stromal/Stem Cells Induced by Three-Dimensional Culture: A Potential Priming Strategy to Improve Their Properties

Author

Gallo, Alessia, primary, Cuscino, Nicola, additional, Contino, Flavia, additional, Bulati, Matteo, additional, Pampalone, Mariangela, additional, Amico, Giandomenico, additional, Zito, Giovanni, additional, Carcione, Claudia, additional, Centi, Claudio, additional, Bertani, Alessandro, additional, Conaldi, Pier Giulio, additional, and Miceli, Vitale, additional

Published
2022
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17. Klebsiella pneumoniae Lipopolysaccharides Serotype O2afg Induce Poor Inflammatory Immune Responses Ex Vivo

Author

Bulati, Matteo, primary, Busà, Rosalia, additional, Carcione, Claudia, additional, Iannolo, Gioacchin, additional, Di Mento, Giuseppina, additional, Cuscino, Nicola, additional, Di Gesù, Roberto, additional, Piccionello, Antonio Palumbo, additional, Buscemi, Silvestre, additional, Carreca, Anna Paola, additional, Barbera, Floriana, additional, Monaco, Francesco, additional, Cardinale, Francesca, additional, Conaldi, Pier Giulio, additional, and Douradinha, Bruno, additional

Published
2021
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18. Extracellular Vesicle-Derived microRNAs of Human Wharton’s Jelly Mesenchymal Stromal Cells May Activate Endogenous VEGF-A to Promote Angiogenesis

Author

Chinnici, Cinzia Maria, primary, Iannolo, Gioacchin, additional, Cittadini, Ettore, additional, Carreca, Anna Paola, additional, Nascari, David, additional, Timoneri, Francesca, additional, Bella, Mariangela Di, additional, Cuscino, Nicola, additional, Amico, Giandomenico, additional, Carcione, Claudia, additional, and Conaldi, Pier Giulio, additional

Published
2021
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19. Conditioned Medium from Human Amnion-Derived Mesenchymal Stromal/Stem Cells Attenuating the Effects of Cold Ischemia-Reperfusion Injury in an In Vitro Model Using Human Alveolar Epithelial Cells

Author

Miceli, Vitale, primary, Bertani, Alessandro, additional, Chinnici, Cinzia Maria, additional, Bulati, Matteo, additional, Pampalone, Mariangela, additional, Amico, Giandomenico, additional, Carcione, Claudia, additional, Schmelzer, Eva, additional, Gerlach, Jörg C., additional, and Conaldi, Pier Giulio, additional

Published
2021
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21. Identification of a Circulating miRNA Signature to Stratify Acute Respiratory Distress Syndrome Patients

Author

Martucci, Gennaro, primary, Arcadipane, Antonio, additional, Tuzzolino, Fabio, additional, Occhipinti, Giovanna, additional, Panarello, Giovanna, additional, Carcione, Claudia, additional, Bonicolini, Eleonora, additional, Vitiello, Chiara, additional, Lorusso, Roberto, additional, Conaldi, Pier Giulio, additional, and Miceli, Vitale, additional

Published
2020
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25. Identification of a Circulating miRNA Signature to Stratify Acute Respiratory Distress Syndrome Patients.

Author

Martucci, Gennaro, Arcadipane, Antonio, Tuzzolino, Fabio, Occhipinti, Giovanna, Panarello, Giovanna, Carcione, Claudia, Bonicolini, Eleonora, Vitiello, Chiara, Lorusso, Roberto, Conaldi, Pier Giulio, and Miceli, Vitale

Subjects
ADULT respiratory distress syndrome, MICRORNA, EXTRACORPOREAL membrane oxygenation, TISSUE remodeling, HIERARCHICAL clustering (Cluster analysis)
Abstract

There is a need to improve acute respiratory distress syndrome (ARDS) diagnosis and management, particularly with extracorporeal membrane oxygenation (ECMO), and different biomarkers have been tested to implement a precision-focused approach. We included ARDS patients on veno-venous (V-V) ECMO in a prospective observational pilot study. Blood samples were obtained before cannulation, and screened for the expression of 754 circulating microRNA (miRNAs) using high-throughput qPCR and hierarchical cluster analysis. The miRNet database was used to predict target genes of deregulated miRNAs, and the DIANA tool was used to identify significant enrichment pathways. A hierarchical cluster of 229 miRNAs (identified after quality control screening) produced a clear separation of 11 patients into two groups: considering the baseline SAPS II, SOFA, and RESP score cluster A (n = 6) showed higher severity compared to cluster B (n = 5); p values < 0.05. After analysis of differentially expressed miRNAs between the two clusters, 95 deregulated miRNAs were identified, and reduced to 13 by in silico analysis. These miRNAs target genes implicated in tissue remodeling, immune system, and blood coagulation pathways. The blood levels of 13 miRNAs are altered in severe ARDS. Further investigations will have to match miRNA results with inflammatory biomarkers and clinical data. [ABSTRACT FROM AUTHOR]

Published
2021
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26. Klebsiella pneumoniae Lipopolysaccharides Serotype O2afg Induce Poor Inflammatory Immune Responses Ex Vivo

Author

Rosalia Busà, Antonio Palumbo Piccionello, Roberto Di Gesù, Matteo Bulati, Nicola Cuscino, Silvestre Buscemi, Claudia Carcione, Anna Paola Carreca, Pier Giulio Conaldi, Francesco Monaco, Bruno Douradinha, Francesca Cardinale, Giuseppina Di Mento, Gioacchin Iannolo, Floriana Barbera, Bulati, Matteo, Busà, Rosalia, Carcione, Claudia, Iannolo, Gioacchin, Di Mento, Giuseppina, Cuscino, Nicola, Di Gesù, Roberto, Piccionello, Antonio Palumbo, Buscemi, Silvestre, Carreca, Anna Paola, Barbera, Floriana, Monaco, Francesco, Cardinale, Francesca, Conaldi, Pier Giulio, and Douradinha, Bruno

Subjects
0301 basic medicine, Microbiology (medical), Serotype, Chemokine, QH301-705.5, Klebsiella pneumoniae, 030106 microbiology, Microbiology, Article, NF-κB, 03 medical and health sciences, chemistry.chemical_compound, Immune system, Antigen, Virology, antimicrobial resistance, Biology (General), Pathogen, immune evasion, biology, lipopolysaccharide, Settore CHIM/06 - Chimica Organica, lipopolysaccharides, biology.organism_classification, 030104 developmental biology, chemistry, nosocomial infection, biology.protein, lipids (amino acids, peptides, and proteins), Ex vivo
Abstract

Currently, Klebsiella pneumoniae is a pathogen of clinical relevance due to its plastic ability of acquiring resistance genes to multiple antibiotics. During K. pneumoniae infections, lipopolysaccharides (LPS) play an ambiguous role as they both activate immune responses but can also play a role in immune evasion. The LPS O2a and LPS O2afg serotypes are prevalent in most multidrug resistant K. pneumoniae strains. Thus, we sought to understand if those two particular LPS serotypes were involved in a mechanism of immune evasion. We have extracted LPS (serotypes O1, O2a and O2afg) from K. pneumoniae strains and, using human monocytes ex vivo, we assessed the ability of those LPS antigens to induce the production of pro-inflammatory cytokines and chemokines. We observed that, when human monocytes are incubated with LPS serotypes O1, O2a or O2afg strains, O2afg and, to a lesser extent, O2a but not O1 failed to elicit the production of pro-inflammatory cytokines and chemokines, which suggests a role in immune evasion. Our preliminary data also shows that nuclear translocation of NF-κB, a process which regulates an immune response against infections, occurs in monocytes incubated with LPS O1 and, to a smaller extent, with LPS O2a, but not with the LPS serotype O2afg. Our results indicate that multidrug resistant K. pneumoniae expressing LPS O2afg serotypes avoid an initial inflammatory immune response and, consequently, are able to systematically spread inside the host unharmed, which results in the several pathologies associated with this bacterium.

Published
2021
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