1. Continuous exposure to low-frequency noise and carbon disulfide: Combined effects on hearing.
- Author
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Venet T, Carreres-Pons M, Chalansonnet M, Thomas A, Merlen L, Nunge H, Bonfanti E, Cosnier F, Llorens J, and Campo P
- Subjects
- Acoustic Stimulation, Analysis of Variance, Animals, Carbon Disulfide blood, Dose-Response Relationship, Radiation, Female, Hearing Tests, Microscopy, Atomic Force, Myosins metabolism, Organ of Corti drug effects, Organ of Corti metabolism, Organ of Corti radiation effects, Organ of Corti ultrastructure, Rats, Rats, Wistar, Spiral Ganglion drug effects, Spiral Ganglion metabolism, Spiral Ganglion radiation effects, Spiral Ganglion ultrastructure, Thiazolidines urine, Time Factors, Carbon Disulfide toxicity, Hearing drug effects, Hearing radiation effects, Noise adverse effects
- Abstract
Carbon disulfide (CS
2 ) is used in industry; it has been shown to have neurotoxic effects, causing central and distal axonopathies.However, it is not considered cochleotoxic as it does not affect hair cells in the organ of Corti, and the only auditory effects reported in the literature were confined to the low-frequency region. No reports on the effects of combined exposure to low-frequency noise and CS2 have been published to date. This article focuses on the effects on rat hearing of combined exposure to noise with increasing concentrations of CS2 (0, 63,250, and 500ppm, 6h per day, 5 days per week, for 4 weeks). The noise used was a low-frequency noise ranging from 0.5 to 2kHz at an intensity of 106dB SPL. Auditory function was tested using distortion product oto-acoustic emissions, which mainly reflects the cochlear performances. Exposure to noise alone caused an auditory deficit in a frequency area ranging from 3.6 to 6 kHz. The damaged area was approximately one octave (6kHz) above the highest frequency of the exposure noise (2.8kHz); it was a little wider than expected based on the noise spectrum.Consequently, since maximum hearing sensitivity is located around 8kHz in rats, low-frequency noise exposure can affect the cochlear regions detecting mid-range frequencies. Co-exposure to CS2 (250-ppm and over) and noise increased the extent of the damaged frequency window since a significant auditory deficit was measured at 9.6kHz in these conditions.Moreover, the significance at 9.6kHz increased with the solvent concentrations. Histological data showed that neither hair cells nor ganglion cells were damaged by CS2 . This discrepancy between functional and histological data is discussed. Like most aromatic solvents, carbon disulfide should be considered as a key parameter in hearing conservation régulations., (Copyright © 2017 Elsevier B.V. All rights reserved.)- Published
- 2017
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