1. Roles of a Cryptochrome in Carbon Fixation and Sucrose Metabolism in the Liverwort Marchantia polymorpha .
- Author
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Li T, Zhang L, Su S, Li S, Zhang J, Yang Z, and Zuo Z
- Subjects
- Amino Acid Sequence, Cryptochromes chemistry, Gene Expression Profiling, Gene Expression Regulation, Plant radiation effects, Glucose metabolism, Light, Marchantia radiation effects, Mutation genetics, Photosynthesis genetics, Photosynthesis radiation effects, Phylogeny, Plant Proteins chemistry, Plant Proteins metabolism, Transcription, Genetic radiation effects, Carbon Cycle radiation effects, Cryptochromes metabolism, Marchantia metabolism, Sucrose metabolism
- Abstract
In vascular plants, cryptochromes acting as blue-light photoreceptors have various functions to adapt plants to the fluctuating light conditions on land, while the roles of cryptochromes in bryophytes have been rarely reported. In this study, we investigated functions of a single-copy ortholog of cryptochrome ( MpCRY ) in the liverwort Marchantia polymorpha. Knock-out of Mp CRY showed that a large number of the mutant plants exhibited asymmetric growth of thalli under blue light. Transcriptome analyses indicated that Mp CRY is mainly involved in photosynthesis and sugar metabolism. Further physiological analysis showed that Mp cry mutant exhibited a reduction in CO
2 uptake and sucrose metabolism. In addition, exogenous application of sucrose or glucose partially restored the symmetrical growth of the Mp cry mutant thalli. Together, these results suggest that Mp CRY is involved in the symmetrical growth of thallus and the regulation of carbon fixation and sucrose metabolism in M. polymorpha .- Published
- 2021
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