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To preserve the Siberian roe deer, the most vulnerable species of the deer family, in conditions of high snow conditions, integrated methods of its biotechnical protection are necessary. The involvement of fallow lands in the biotechnical crop rotation, the creation of large forage areas is the basic basis for the conservation of this biological species in the anomalously critical periods of the winter cycle.

Livestock grazing is one of the most widespread types of anthropogenic land use, even occurs in many protected areas and has become a threat to wildlife worldwide. Understanding livestock-wildlife interactions is crucial for rare large carnivores conservation. In China, free-ranging cattle within forests degrade the habitat of the tigers (Panthera tigris) and leopards (Panthera pardus), but quantitative assessments of how livestock affect the spatial and temporal use by the major ungulate prey of the two endangered felids are very limited. This study aimed to examine the interactions of several sympatric wildlife species with livestock at a fine spatiotemporal scale in a human-dominated forest landscape. Based on a large-scale camera-trapping data across the China-Russia border, we used N-mixture models, two-species occupancy models and activity pattern overlap to understand the effects of cattle grazing on three ungulate species (sika deer Cervus nippon, wild boars Sus scrofa and roe deer Capreolus pygargus). Spatially, with cattle activity increasing, wild boar and roe deer had different degrees of decline in the intensity of habitat use. Sika deer were displaced as more cattle encroached on forest habitat. Temporally, in the presence of cattle, wild boar and sika deer decreased their activities in the day. In addition, three wild ungulates trend to exhibit lower spatiotemporal overlap with cattle at shared camera sites. Our study shows that wildlife species may reduce the probability of habitat use by spatial avoidance and changing the daily activity patterns. We underscore that fine-scale (i.e. camera-site level) spatiotemporal avoidance is likely a key component of co-occurrence between livestock and the sympatry of competing ungulates inhabiting forest ecosystems. Given prey were depressed, efforts to minimize the livestock disturbance on these species need to be considered to ensure their sustained recoveries.

The increasing animosity towards wolves (Canislupus) by livestock-keeping nomads in Mongolia and the accompanying conflicts highlight the urgent need for knowledge about the feeding behavior of wolves, since information on the feeding ecology of wolves in Mongolia is rare, especially in the mountain taiga and mountain forest steppe regions of Northern Mongolia. Those regions are characterized by a relatively high wildlife diversity and are sparsely populated by humans. To face this problem, 137 wolf scats were collected in the Khentii Mountain range in Northern Mongolia between 2008 and 2012. Almost all wolf faeces contained remnants of wild ungulates, which made up 89% of the consumed biomass. Siberian roe deer (Capreoluspygargus) was the most important and positively selected prey species. It was followed by red deer (Cervuselaphus) and wild boar (Susscrofa), which was negatively selected by wolves. Wolves also fed on buffer prey species such as lagomorphs and small mammals. No evidence of domestic ungulates was found in the wolf diet. Thus, near-natural habitats with a diverse fauna of wild animals are important to limit livestock depredation.

There is evidence that cattle grazing in forests limits big cat abundance. There is concern, too, about competition with wild ungulate prey through bottom-up effects on vegetation. Hence, there have been calls to remove or control forest livestock grazing in aid of restoring endangered large carnivores and their prey. To help inform scientific debate and decision making, we explored cattle-vegetation-prey dynamics in Northeast Tiger and Leopard National Park, northeast China and present a reappraisal of livestock grazing in the context of coexistence and an integrated approach to land use in China. Inside long-term forest grazing enclosures, wild boar (Sus scrofa) density increased with cattle density. Roe deer (Capreolus pygargus) density and occurrence probability of wild boar and roe deer were not influenced by cattle density. Wild ungulate densities were not related with the number of annual shoots, i.e., forage plant abundance. The presence/absence and abundance of annual shoots was not related with cattle density. Wild ungulate density had mixed associations with vegetation structure; arbor tree diameter at breast height (DBH) and habitat “openness” increased with cattle density. Finally, inside and outside enclosures had different vegetation characteristics and wild boar densities, while roe deer densities were equal. We conclude that cattle density and associated changes to vegetation have positive, negative, and neutral effects on two wild ungulate prey species. Each of these factors warrant consideration in evidence-based management decisions in regard to regulating ungulate community composition to support different large predators as preferred prey in core areas and corridors of habitats.

The construction and continued use of transportation infrastructure, specifically roads, has had a significant global impact on biodiversity and the environment. The Republic of Korea, or South Korea, has a road density of 1.13 km/km2. So far, three nationwide studies about vertebrate road-killed species have been reported, showing bias towards medium to large mammals, the most common victims being the Korean water deer (Hydropotes inermis), Korean hare (Lepus coreanus), Siberian roe deer (Capreolus pygargus), and the common raccoon dog (Nyctereutes procyonoides). Road-kills, or wildlife-vehicle collisions (WVCs), tend to occur in or near preferred habitat types or in highly fragmented areas, with roads additionally being linked to habitat fragmentation and loss. Alongside WVCs and habitat effects, information about other adverse effects on biodiversity is scant, although there are reports that heavy metals and other pollutants from road runoff impact marine biodiversity, vegetation, soil, and groundwater. Furthermore, roads have been linked to a prevalence of invasive plant species. To mitigate road impacts, the South Korean government has constructed, with mixed results, 530 wildlife crossing structures, mainly including overpasses and tunnels. To mitigate road impacts more effectively, the country will need more construction, monitoring, and consistent management of wildlife crossing structures. Further, incorporating plans for wildlife crossing structures in early stages of road development will be required.

Habitat loss and fragmentation are serious threats to biodiversity worldwide. In order to promote animal movement and gene flow between habitat islands, it is important to identify and evaluate the ecological network of species. In Tieli Forestry Bureau (TFB) in Northeast China, we simulated and evaluated the ecological network of roe deer, aiming to provide theoretical support to population conservation. By using three sources of roe deer occurrence data and 15 environmental variables, we simulated the suitability of habitats using the Maximum Entropy Model (MaxEnt). Then, the corridor design model Linkage Mapper was used to identify and evaluate the linkages between habitat cores which are defined by the home range size of roe deer. The results showed that, of the terrain, land cover, vegetation, and anthropogenic interference factors, the anthropogenic interference factors played the most important and complex role in roe deer habitat selection, creating large-area suitable habitats by farming. Two innovative variables of farmland-forest edge length/patch perimeter (FFE:PP) and viewshed introduced in simulation contributed 8.5% and 2.3%, respectively. Surrounded by the unsuitable habitats, the suitable habitats mainly distributed along both sides of the river, covering 41.8% of the total area of TFB. The ecological network of roe deer was composed of 11 habitat cores and 17 linkages. Three linkages connecting the key habitat cores had low quality and linkage suitability ratios, which were probably due to barrier effect of the HeHa expressway intersecting with the linkages. Our study suggests the priority areas for protecting roe deer and the key locations where the wildlife road-crossing structures or animal guidance measures are needed.

The Changbaishan reserve and the forests around it are one of the priority areas for Amur tiger (Panthera tigris altaica) recovery in northeastern China. Previous habitat suitability analyses only took the ecological requirements of tigers into consideration, so this study aims to determine habitat suitability for a tiger-prey community in the region, by analysing ungulate prey availability and habitat suitability for both predator and prey. Three prey species were found, using the snow tacking method: red deer (Cervus canadensis xanthopygus), wild boar (Sus scrofa), and roe deer (Capreolus pygargus). Habitat suitability was evaluated for tigers, red deer, and wild boar, using a multi-criteria evaluation (MCE) process. MCE results showed that (1) habitat suitability is generally low outside the reserve for all three species; (2) suitability values were the lowest for tigers due to high intensity of human impact in the area, with suitable habitat restricted to the centre of the reserve; and (3) red deer and wild boar would find pockets of suitable habitat outside the reserve. A combination of low forest quality and high human impact intensity imposes significant environmental pressure to those ungulates. To recover tiger population in Changbaishan, forest quality and human impacts should be properly managed, which should increase prey availability.

A Correction to this paper has been published

Wildlife–vehicle collisions (WVCs) are increasing worldwide, posing a threat to both humans and wildlife in terms of human injury and loss of wildlife. Understanding the temporal and spatial non-randomness of WVCs is an essential process for effective WVC management. Roe deer (Capreolus pygargus) is the most prevalent wildlife species in WVCs on Jeju Island, South Korea. This study analyzed individual temporal patterns and spatial hotspots (clusters) based on roe deer–vehicle collisions (RDVC) on Jeju Island from 2014 to 2017. RDVCs occur frequently from July to October, and RDVCs are higher in male deer than with females in all seasons, except in winter. The clusters in the high-risk periods were more regionally distributed compared to the clusters in the low-risk periods. Regardless of the period, some road sections have been identified as crucial clusters that should be given priority for road fencing to mitigate human-deer conflicts. Our findings could also provide road administrators with practical options for management such as temporarily imposing vehicle speed limits and installing signboards.

We sequenced the complete mitochondrial genome (mitogenome) of Siberian Roe Deer, Capreolus pygargus, in China by the shotgun genome-skimming methods. The mitogenome of C. pygargus is totally 16,353 bp in length and contains 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a control region. The sequence has a higher A + T content of 63.4% than G + C 36.6% and with a base composition of 33.4% A, 23.2% C, 13.4% G, and 30.0% T. All of the 13 PCGs initiate a typical ATN codon except Nd4L with GTG. Six PCGs terminate with a TAA codon, while Cyt b, Atp8, and Nd1 terminate with AGA, TAG, and TA–, respectively. Whereas, Cox3, Nd2, Nd3, and Nd4 terminate with a single T-. The phylogenetic trees of the subfamily Capreolinae with 13 PCGs indicated that Capreolus species were well-supported as a monophyletic group, which is sister to the clade of Hydropotes with well-support.

It is necessary to estimate the population abundance of deer for managing their populations. However, most estimates are from high-density populations inhabiting the forests of North America or Europe, there is currently a lack of necessary knowledge regarding low-density deer populations in different forest habitats. In this article, we used fecal DNA based on the capture-mark-recapture method to estimate the population abundance of Siberian roe deer (Capreolus pygargus) in Liangshui National Nature Reserve in the Lesser Xing&rsquo, an Mountains, northeast China, where the deer population was found to be of a low density by limited studies. We used a robust survey design to collect 422 fecal pellet groups in 2016 and extracted DNA from those samples, generating 265 different genotypes, we thus identified 77 deer individuals based on six microsatellite markers (Roe1, Roe8, Roe9, BM757, MB25 and OarFCB304). With capture and recapture records of these 77 individuals, the abundance of roe deer was estimated to be 87 deer (80&ndash, 112, 95% CI) using the Program CAPTURE. Using an effective sampling area which resulted from the mean maximum recapture distance (MMRD), we converted the population abundance to a density of 2.9 deer/km2 (2.7&ndash, 3.7, 95% CI). Our study estimated the roe deer population abundance by a feces-based capture-mark-recapture approach in northeast China, successfully demonstrating the applicability of non-invasive genetic sampling in monitoring populations of deer in this area, which contributes to the development of low-density deer population ecology and management.

This study examined and compared the branching pattern of the aortic arch (AA) and its major branches in the Siberian roe deer (Capreolus pygargus) on Jeju Island (Jeju roe deer [JRD]) with those in the roe deer of the Korean peninsula (mainland roe deer [MRD]). Seven of the nine expected types was observed in the arterial silicone casts of 29 deer (10 males, 19 females). The JRD was identical to the MRD in that absence of the typical pattern; however, the main three pattern types differed between the two. This difference resulted from differences in the branching patterns of the right subclavian artery and costocervical trunk. In conclusion, the JRD has different type of AA from the MRD.

Sequence analysis of the mtDNA cytochrome b gene (974 bp) was performed using 139 roe deer specimens from different regions of the European part of Russia and Ukraine. The data obtained showed that, at present, both European and Siberian roe deer inhabit this part of the range with predominance of the later: about 60% of individuals carry various Siberian haplotypes, most of which are similar to those in the populations of Capreolus pygargus from the Urals, Siberia, and the Far East. A great variety of mtDNA haplotypes of Siberian roe deer in Eastern Europe is undoubtedly caused by the heterogeneity of founder individuals (immigrants) that were imported from different parts of Asia. Some problems of coexistence of closely related species are discussed.

The paper is devoted to studying of the relations between the formation of “private” hunting grounds, the increase in the density of ungulates and the decline in the numbers of wolves, and the role of other factors determining the abundance of these game animals. Six ungulate species constantly live in the Saratov Region, namely: the elk ( Alces alces L.), the red deer ( Cervus elaphus L.), the axis deer ( Cervus nippon Temminck), the roe deer ( Capreolus capreolus L.), the Tartarian roe ( Capreolus pygargus Pallas), and the wild boar ( Sus scrofa L.). All these species, except thered deer, are commercial. The roe deer is a rare species listed in the Red Data Book of the Saratov Region. The population dynamics of the ungulates and wolf in the described region was studied using winter field records and official data on animals shooting for the period from 2001 till 2018. The abundance of all the ungulate species for this period has increased significantly, whilst the numbers of wolves, on the contrary, have decreased. The influence of several factors, both natural and anthropogenic, on the population dynamics of the mentioned species was analyzed. First of all, the impact of official hunting on the game species was considered. Of all the factors examined, only one influenced directly the population dynamics of wild ungulates in theSaratov region at the beginning of this century. The very strong direct correlation between the increase in the area of hunting grounds assigned to private hunting managers, and the increase in the numbers of wild ungulates undoubtedly speaks for their causal relationship.

This study examined the branching pattern of the aortic arch (AA) and its major branches in the Siberian roe deer (Capreolus pygargus Pallas, 1771) from South Korea. A total of eight of the nine expected types, based on the branching site and bilateral levels of the costocervical trunk (CCT) and subclavian artery (SB), were observed in the arterial silicone casts of 35 deer (16 males, 19 females). This deer has no typical type. The three most common types were present in 28.6, 25.7 and 20.0% of cases and resulted from different branching patterns of the left CCT and left SB. These results suggest that the Siberian roe deer in the Korean peninsula has various AA branching patterns, which differs from other ruminants.

A molecular-genetic analysis of the nucleotide sequences of the cytochrome b gene (1140 base pairs) of the mitochondrial DNA and 17 microsatellite loci of eight samples of roe deer from the Samara forest of Dnipropetrovsk oblast (Ukraine) was carried out. For comparison, 212 corresponding mtDNA sequences of the Siberian and European roe deer and data on the variability of microsatellite markers in 49 representatives of these species were included in the study. It was noted that all the analyzed mitochondrial sequences of individuals from the Samara forest are characteristic of the Siberian roe Capreolus pygargus Pallas, 1771. Four haplotypes were described, all of which belonged to the haplogroup typical for the western part of the range of C. pygargus. A fragment analysis of the microsatellite loci of nuclear DNA confirmed the identification of the investigated group with the Siberian species.

Forestry practices can lead to changes in wildlife habitat that could have lasting effects on the distribution of species. Hence, the aim of this study was to determine the effects of forestry practices on habitat variables and habitat use by mammals in three different stands (control, clear-cut and clear-cut with reserves) in a Japanese larch Larix kaempferi plantation from April to October in 2016 and 2017. We recorded the presence of field signs for the following six mammal species: Korean hare Lepus coreanus, raccoon dog Nyctereutes procyonoides, Eurasian badger Meles meles, water deer Hydropotes inermis, roe deer Capreolus pygargus and wild boar Sus scrofa. Habitat variables significantly differed among three stands. Field sign (feces, foot prints and feeding signs) abundances of mammals showed significant differences between the control and clear-cut stands. Moreover, field sign abundances of all species were highest in the clear-cut with reserves stand. We calculated the impact of clear-cutting on habitat use by mammals using GLMMs. Both clear-cutting in the models were significant and had positive coefficients on habitat use by Korean hare, raccoon dog, deer and wild boar. In Eurasian badger models, only clear-cutting with reserves show positive impacts on habitat use. Habitat selection by forest edge species such as Korean hare and deer showed dependence on understory structure. Abundant understory in the clear-cut with reserves stand may not only provide vegetative food and cover for herbivores but also small prey species for omnivores and carnivores. The results of our study show that forest understory is one of the most instrumental factors mitigating the effects of clear-cutting on habitat use by mammal and it should, therefore, be given due importance in forestry practices.

Background Anaplasma spp. are tick-borne Gram-negative obligate intracellular bacteria that infect humans and a wide range of animals. Anaplasma capra has emerged as a human pathogen; however, little is known about the occurrence and genetic identity of this agent in wildlife. The present study aimed to determine the infection rate and genetic profile of this pathogen in wild animals in the Republic of Korea. Methods A total of 253 blood samples [198 from Korean water deer (Hydropotes inermis argyropus), 53 from raccoon dogs (Nyctereutes procyonoides) and one sample each from a leopard cat (Prionailurus bengalensis) and a roe deer (Capreolus pygargus)] were collected at Chungbuk Wildlife Center during the period 2015–2018. Genomic DNA was extracted from the samples and screened for presence of Anaplasma species by PCR/sequence analysis of 429 bp of the 16S rRNA gene marker. Anaplasma capra-positive isolates were genetically profiled by amplification of a longer fragment of 16S rRNA (rrs) as well as partial sequences of citrate synthase (gltA), heat-shock protein (groEL), major surface protein 2 (msp2) and major surface protein 4 (msp4). Generated sequences of each gene marker were aligned with homologous sequences in the database and phylogenetically analyzed. Results Anaplasma capra was detected in blood samples derived from Korean water deer, whereas samples from other animal species were negative. The overall infection rate in tested samples was 13.8% (35/253) and in the water deer the rate was 17.8% (35/198), distributed along the study period from 2015 to 2018. Genetic profiling and a phylogenetic analysis based on analyzed gene markers revealed the occurrence of two distinct strains, clustered in a single clade with counterpart sequences of A. capra in the database. Conclusions Anaplasma capra infection were detected in Korean water deer in the Republic of Korea, providing insight into the role of wildlife as a potential reservoir for animal and human anaplasmosis. However, further work is needed in order to evaluate the role of Korean water deer as a host/reservoir host of A. capra.

Background Small ruminants are important hosts for various tick species and tick-associated organisms, many of which are zoonotic. The aim of the present study was to determine the presence of tick-borne protozoans and bacteria of public health and veterinary significance in goats and wild Siberian roe deer (Capreolus pygargus) from Heilongjiang Province, northeastern China. Methods The occurrence of piroplasms, Anaplasma phagocytophilum, A. bovis, A. marginale, A. capra, A. ovis, Ehrlichia spp. and spotted fever group rickettsiae was molecularly investigated and analyzed in 134 goats and 9 free ranging C. pygargus living in close proximity. Results Piroplasm DNA was detected in 16 (11.9%) goats and 5 C. pygargus. Sequence analysis of 18S rRNA sequences identified 3 Theileria species (T. luwenshuni, T. capreoli and T. cervi). Four Anaplasma species (A. ovis, A. phagocytophilum, A. bovis and A. capra) were identified in goats and C. pygargus. Anaplasma ovis and A. bovis were detected in 11 (8.2%) and 6 (4.5%) goats, respectively; A. phagocytophilum, A. bovis and A. capra were found in 3, 7 and 3 C. pygargus, respectively. Sequence analysis of 16S rRNA sequences revealed the presence of 5 different genetic variants of A. bovis in goats and C. pygargus, while the analysis of 16S rRNA and gltA sequence data showed that A. capra isolates identified from C. pygargus were closely related to the genotype identified from sheep and Haemaphysalis qinghaiensis, but differed with the genotype from humans. Anaplasma/Theileria mixed infection was observed in 2 (1.5%) goats and 5 C. pygargus, and co-existence involving potential zoonotic organisms (A. phagocytophilum and A. capra) was found in 2 C. pygargus. All samples were negative for A. marginale, Ehrlichia spp. and SFG rickettsiae. Conclusions These findings report the tick-borne pathogens in goats and C. pygargus, and a greater diversity of these pathogens were observed in wild animals. Three Theileria (T. luwenshuni, T. capreoli and T. cervi) and four Anaplasma species (A. ovis, A. phagocytophilum, A. bovis and A. capra) with veterinary and medical significance were identified in small domestic and wild ruminants. The contact between wild and domestic animals may increase the potential risk of spread and transmission of tick-borne diseases.

Reductive acetogenesis by homoacetogens represents an alternative pathway to methanogenesis to remove metabolic hydrogen during rumen fermentation. In this study, we investigated the occurrence of homoacetogen in the rumens of pasture-fed roe deer (Capreolus pygargus) and sika deer (Cervus nippon) fed either oak-leaf-based (tannin-rich, 100 mg/kg dried matter), corn-stover-based, or corn-silage-based diets, by using formyltetrahydrofolate synthetase (FTHFS) gene sequences as a marker. The diversity and richness of FTHFS sequences was lowest in animals fed oak leaf, indicating that tannin-containing plants may affect rumen homoacetogen diversity. FTHFS amino acid sequences in the rumen of roe deer significantly differed from those of sika deer. The phylogenetic analyses showed that 44.8% of sequences in pasture-fed roe deer, and 72.1%, 81.1%, and 37.5% of sequences in sika deer fed oak-leaf-, corn-stover-, and corn-silage-based diets, respectively, may represent novel bacteria that have not yet been cultured. These results demonstrate that the rumens of roe deer and sika deer harbor potentially novel homoacetogens and that diet may influence homoacetogen community structure.

Patterns of habitat use profoundly influence interactions among wildlife species and ecological communities, the sustainability of species and the stability of populations. The Eurasian lynx Lynx lynx, sable Martes zibellina, moose Alces alces, roe deer Capreolus pygargus and mountain hare Lepus timidus are sympatric carnivore and herbivore species in Hanma Nature Reserve in the Greater Khingan Mountains, northeastern China. We conducted snow track sample plot surveys of these mammals during the winter of 2012–2013 and analyzed habitat selection characteristics and interspecific interactions. We screened the preferred habitat variables of each species and predicted their potential occurrence probability by generalized linear mixed model (GLMM) and further, selected the best model of each species that incorporated other species' potential occurrence probability in GLMM. Our results showed that when we only considered habitat factors in the model: 1) river valley was the most favored habitat factor in winte...

A new species of tapeworm, Taenia lynciscapreoli sp. n. (Cestoda, Cyclophyllidea), is described from the Eurasian lynx (Lynx lynx), the main definitive host, and the roe deer (Capreolus capreolus and Capreolus pygargus), the main intermediate hosts, from Finland and Russia (Siberia and the Russian Far East). The new species was found once also in the wolf (Canis lupus) and the Eurasian elk/moose (Alces alces), representing accidental definitive and intermediate hosts, respectively. The conspecificity of adult specimens and metacestodes of Taenia lynciscapreoli sp. n. in various host species and regions, and their distinction from related species of Taenia, was confirmed by partial nucleotide sequences of the mitochondrial cytochrome c oxidase subunit 1 gene. Morphologically, Taenia lynciscapreoli sp. n. can be separated unambiguously from all other species of Taenia by the shape of its large rostellar hooks, particularly the characteristically short, wide and strongly curved blade. If the large rostellar hooks are missing, Taenia lynciscapreoli may be separated from related species by a combination of morphological features of mature proglottids. It is suggested that Taenia lynciscapreoli has been present in published materials concerning the tapeworms of Lynx lynx and Lynx pardinus in Europe, but has been misidentified as Taenia pisiformis (Bloch, 1780). Taenia lynciscapreoli sp. n. has not been found in lynx outside the range of roe deer, suggesting a transmission pathway based on a specific predator–prey relationship. The present study applies a novel, simple approach to compare qualitative interspecific differences in the shape of rostellar hooks.

This study predicts future land-use changes and the resulting changes in habitat quality, suggesting a method for establishing land-use management to ensure sustainable wildlife habitats. The conservation effects were verified in terms of wild animal habitat quality according to the designation of protected areas. Land-use change until 2050 was predicted using the Dyna-Conversion of Land Use Change and its effects (Dyna-CLUE) model for Jeju Island, Korea, and the change in the quality of roe deer habitats was predicted using the Integrated Valuation and Environmental Services and Tradeoffs (InVEST) model. Results indicate that, compared to 2030, urbanized area increased by 42.55 km2, farmland decreased by 81.36 km2, and natural area increased by 38.82 km2 by 2050. The average habitat quality on Jeju Island was predicted to decrease from 0.306 in 2030 to 0.303 in 2050. The average habitat quality ranged from 0.477 in 2030 to 0.476 in 2050 in protected areas and 0.281 in 2030 to 0.278 in 2050 outside protected areas. Habitat quality in protected areas was relatively high, and its reduction was limited. Areas with lower habitat quality need approaches such as expanding greenery and improving its quality. By establishing appropriate land-use plans by predicting habitat quality, wildlife habitats can be better maintained and protected, which is a primary goal of green infrastructure.

Due to the multiple causes, the population of roe deer has declined significantly. In this study, we analyzed the complete mitogenome of Capreolus pygargus bedfordi, whose genome was 16,357 bp long. There were 13 protein-coding genes (PCG), two ribosomal RNA genes (12S rRNA and 16S rRNA), 22 transfer RNA genes, and one control region. Nine PCGs started with ATG, while NAD2, NAD3, and NAD5 genes commenced with ATA, and ND4L began with GTG. ND6 and eight tRNA genes were encoded on the L-strand. These results provide newer molecular information, which contribute to its molecular and phylogenetic studies, and genetic diversity conservation.

Capreolus pygargus (Pallas, 1771) —Siberian Roe Deer Cervus pygargus Pallas, 1771 p.453; Type locality- Bugulma uplands, source of River Sok, Russia. Cervus pygargus var. mantschurucus Noack, 1889 p.15. Capreolus bedfordi Thomas, 1908 p.645; Type locality- 100 mile NW of Taiyuen Fu (Taiyuan), Shanxi Province, China. Capreolus bedfordi bedfordi: Kuroda, 1917 p.363; (Gwangneung near Seoul): Kishida & Mori, 1931 p.380. Capreolus pygargus manchuricus: Kishida & Mori, 1931 p.380 Capreolus capreolus ochracea Barclay, 1935 p.626; Type locality- Korea; Kuroda, 1938 p.7. Capreolus pygargus pygargus: Kuroda, 1938 p.7. Capreolus capreolus bedfordi: Tate, 1947 p.337; Ellerman & Morrison-Scott, 1951 p.373; Won, 1958 p.432; Won, 1967 p.52; Won, 1968 p.367; Corbet, 1978 p.204; Yoon, 1992 p.133. Capreolus capreolus pygargus: Won, 1958 p.431; Won, 1968 p.362; Yoon, 1992 p.133. Capreolus capreolus: Won, 1968 p.360. Capreolus pygargus: Han, 1994 p.46; Won & Smith, 1999 p.23; Oh, 2004b p.269. Capreolus pygargus tianschanicus: Oh, 2004b p.270; Jo et al., 2012 p.252. Range: The range of C. pygargus covers all of the Korean Peninsula and Jeju Island, the latter having the most abundant population (Fig. 69). Remarks: A single subspecies is supposed to occur in Korea (Oh 2004b). However, while DNA analysis indicated a small genetic difference between the peninsular and continental populations, the Jeju Island population had substantial genetic differences compared to the peninsular and continental populations (Koh & Yang 2000; Koh et al. 2012a). Therefore, Koh et al. (2012a) suggested the population on Jeju Island should be recognized as a distinct subspecies. Xiao et al. (2007) showed that the average mtDNA distance between northeastern China and European roe deer (5.8%) was more than twice that between northeastern China and Korean roe deer (2.7%). The authors concluded that roe deer of northeastern China, Korea and Siberia were a single species (Capreolus pygargus), distinct from the European roe deer (Capreolus capreolus). Conservation status: The continuous use in folk medicine of antlers of roe deer caused a decline in populations in Korea. Due to active conservation efforts, the density of roe deer on Jeju Island in 2007 increased to 5.33 individuals per km 2 (Kim et al. 2007). On the other hand, the density of mainland populations in South Korea declined to 1.86 individuals per km 2 (unpublished data from Wildlife Survey by the NIBR). Siberian roe deer on Jeju Island are now considered an ‘agricultural pest’ because of their damage to crops (Oh 2011). Seoul, Ulsan, Gwangju Metropolitan governments and Jeollanam Province Provincial government designated this species as a Provincially Protected Species. Like mainland populations of Siberian roe deer, populations of most wildlife game species declined or even became extirpated in North Korea by illegal poachers using snares (William Duckworth, IUCN, Pers. Comm.)., Published as part of Jo, Yeong-Seok, Baccus, John T. & Koprowski, John L., 2018, Mammals of Korea: a review of their taxonomy, distribution and conservation status, pp. 1-216 in Zootaxa 4522 (1) on pages 105-107, DOI: 10.11646/zootaxa.4522.1.1, http://zenodo.org/record/2610198, {"references":["Pallas, P. S. (1771) Reise durch verschiedene Provinzen des Russischen Reichs Fol. 1. Kayserliche Academie der Wissenschaften, St. Petersburg, 524 pp. [in German]","Noack, T. (1889) zur saugetierfauna der mandschurischen subregion. Humboldt; Monatsschrift fur die gesamten Naturwissenschaften, 8, 8 - 16. [in German]","Thomas, O. (1908 a) The Duke of Bedford's Zoological Exploration in Eastern Asia. - VI. List of mammals from the Shantung Peninsula, N. China. 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The management of multi-use forests often drives forest fragmentation, which leads to decreased habitat areas and quality. We explored suitable habitat distributions of cervids to evaluate the conflict between small-scale human management and large-scale habitat conservation in human-disturbed forest landscapes. We estimated the potential habitat of roe deer (Capreolus pygargus) and determined the contribution of multiple environmental factors to habitat distribution using the presence of roe deer (N = 106) in a maximum entropy (MaxEnt) model. We simulated changes in the suitable habitat and characteristics of landscape patterns based on three forest management area scenarios. The results showed that the potential suitable habitat for roe deer was located mainly in the east. The variables affecting habitat suitability were similar in different scenarios, and included distance to farmland, settlements, rivers and management areas, and elevation. Distance to the management area was found to affect habitat suitability with a contribution probability from 4% to 6%. With an increase in the management area, the suitable habitat decreased. Landscape indexes showed that habitat quality decreased with management area expansion, but patch fragmentation was not aggravated. The expansion of the management area increased the range of human disturbance and had a negative impact on habitat area and quality, which adversely affected the environmental suitability for roe deer.

Patterns of expansion of geographical ranges of red deer and Siberian roe deer in Yakutia during the 20th century are considered. The area inhabited by red deer in Yakutia has increased almost fourfold over the past 50 years, with an expansion of over 200000 km2 toward the northwest. The geographical range of roe deer in the Lena–Amga interfluve has also changed considerably during the same period. Roe deer sightings in the subarctic zone were reported. The structure of geographical ranges of the species under investigation in different ecological and geographical regions of Yakutia is discussed, and the factors influencing the transformation of geographical range borders are characterized.