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428 results on '"Capparelli, Rosanna"'

3. Phage-resistance alters Lipid A reactogenicity: a new strategy for LPS-based conjugate vaccines against Salmonella Rissen.

Author

Cuomo, Paola, Medaglia, Chiara, Casillo, Angela, Gentile, Antonio, Fruggiero, Carmine, Corsaro, Maria Michela, and Capparelli, Rosanna

Subjects
SALMONELLA diseases, FOODBORNE diseases, SALMONELLA enterica, VACCINE development, DRUG resistance in bacteria
Abstract

Salmonella enterica serovar Rissen (S. Rissen) is an emerging causative agent of foodborne diseases. The current emergence of antibiotic resistance makes necessary alternative therapeutic strategies. In this study, we investigated the potential of a phage-resistant strain of S. Rissen (RR) as a tool for developing an effective lipopolysaccharide (LPS)-based vaccine. The LPS O-antigen is known to play critical roles in protective immunity against Salmonella. However, the high toxicity of the LPS lipid A moiety limits its use in vaccines. Here, we demonstrated that the acquisition of bacteriophage resistance by S. Rissen leads to structural modifications in the LPS structure. Using NMR and mass spectrometry, we characterized the LPS from phage-resistant strains as a smooth variant bearing under-acylated Lipid A portions (penta- and tetra-acylated forms). We then combined RT-qPCR and NMR-based metabolomics to explore the effects of phage resistance and LPS modification on bacterial fitness and virulence. Finally, we conducted in vivo studies to determine whether lysogeny-induced remodeling of LPS affects the host immune response. Results revealed that the under-acylated variant of LPS from RR attenuates the inflammatory response in BALB/c mice, while eliciting a specific antibody response that protects against S. Rissen (RW) infection. In conclusion, our findings suggest that phage resistance, through lipid A modification, may offer a novel strategy for reducing LPS toxicity, highlighting its potential as a promising biological approach for developing LPS-based vaccines against Salmonella infections. [ABSTRACT FROM AUTHOR]

Published
2024
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4. Extracellular Self-DNA Effects on Yeast Cell Cycle and Transcriptome during Batch Growth

Author

Palomba, Emanuela, primary, Chiusano, Maria Luisa, additional, Monticolo, Francesco, additional, Langella, Maria Chiara, additional, Sanchez, Massimo, additional, Tirelli, Valentina, additional, de Alteriis, Elisabetta, additional, Iannaccone, Marco, additional, Termolino, Pasquale, additional, Capparelli, Rosanna, additional, Carteni, Fabrizio, additional, Incerti, Guido, additional, and Mazzoleni, Stefano, additional

Published
2024
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5. Extracellular DNA secreted in yeast cultures is metabolism-specific and inhibits cell proliferation

Author

de Alteriis, Elisabetta, primary, Incerti, Guido, additional, Cartenì, Fabrizio, additional, Chiusano, Maria Luisa, additional, Colantuono, Chiara, additional, Palomba, Emanuela, additional, Termolino, Pasquale, additional, Monticolo, Francesco, additional, Esposito, Alfonso, additional, Bonanomi, Giuliano, additional, Capparelli, Rosanna, additional, Iannaccone, Marco, additional, Foscari, Alessandro, additional, Landi, Carmine, additional, Parascandola, Palma, additional, Sanchez, Massimo, additional, Tirelli, Valentina, additional, de Falco, Bruna, additional, Lanzotti, Virginia, additional, and Mazzoleni, Stefano, additional

Published
2023
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7. Polystyrene Microplastics Exacerbate Candida albicans Infection Ability In Vitro and In Vivo.

Author

Maione, Angela, Norcia, Mariangela, Sinoca, Marica, Galdiero, Marilena, Maselli, Valeria, Feola, Antonia, Carotenuto, Rosa, Cuomo, Paola, Capparelli, Rosanna, Guida, Marco, and Galdiero, Emilia

Subjects
CANDIDIASIS, MICROPLASTICS, POLYSTYRENE, GREATER wax moth, CANDIDA albicans, ANIMAL health, INSECT nematodes
Abstract

Plastic pollution is an important environmental problem, and microplastics have been shown to have harmful effects on human and animal health, affecting immune and metabolic physiological functions. Further, microplastics can interfere with commensal microorganisms and exert deleterious effects on exposure to pathogens. Here, we compared the effects of 1 µm diameter polystyrene microplastic (PSMPs) on Candida albicans infection in both in vitro and in vivo models by using HT29 cells and Galleria mellonella larvae, respectively. The results demonstrated that PSMPs could promote Candida infection in HT29 cells and larvae of G. mellonella, which show immune responses similar to vertebrates. In this study, we provide new experimental evidence for the risk to human health posed by PSMPs in conjunction with Candida infections. [ABSTRACT FROM AUTHOR]

Published
2024
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22. Harzianic Acid Activity against Staphylococcus aureus and Its Role in Calcium Regulation.

Author

Staropoli, Alessia, Cuomo, Paola, Salvatore, Maria Michela, De Tommaso, Gaetano, Iuliano, Mauro, Andolfi, Anna, Tenore, Gian Carlo, Capparelli, Rosanna, and Vinale, Francesco

Subjects
NUCLEAR magnetic resonance, CALCIUM, NASAL mucosa
Abstract

Staphylococcus aureus is a Gram-positive bacterium, which can be found, as a commensal microorganism, on the skin surface or in the nasal mucosa of the human population. However, S. aureus may become pathogenic and cause severe infections, especially in hospitalized patients. As an opportunistic pathogen, in fact, S. aureus interferes with the host Ca2+ signaling, favoring the spread of the infection and tissue destruction. The identification of novel strategies to restore calcium homeostasis and prevent the associated clinical outcomes is an emerging challenge. Here, we investigate whether harzianic acid, a bioactive metabolite derived from fungi of the genus Trichoderma, could control S. aureus-induced Ca2+ movements. First, we show the capability of harzianic acid to complex calcium divalent cations, using mass spectrometric, potentiometric, spectrophotometric, and nuclear magnetic resonance techniques. Then, we demonstrate that harzianic acid significantly modulates Ca2+ increase in HaCaT (human keratinocytes) cells incubated with S. aureus. In conclusion, this study suggests harzianic acid as a promising therapeutical alternative against diseases associated with Ca2+ homeostasis alteration. [ABSTRACT FROM AUTHOR]

Published
2023
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25. MicroRNA-223 controls susceptibility to tuberculosis by regulating lung neutrophil recruitment

Author

Dorhoi, Anca, Iannaccone, Marco, Farinacci, Maura, Fae, Kellen C., Schreiber, Jorg, Moura-Alves, Pedro, Nouailles, Geraldine, Mollenkopf, Hans-Joachim, Oberbeck-Muller, Dagmar, Jorg, Sabine, Heinemann, Ellen, Hahnke, Karin, Lowe, Delia, Nonno, Franca Del, Goletti, Delia, Capparelli, Rosanna, and Kaufmann, Stefan H.E.

Subjects
Tuberculosis -- Development and progression -- Genetic aspects -- Research, MicroRNA -- Physiological aspects -- Research, Genetic susceptibility -- Research, Health care industry
Abstract

The molecular mechanisms that control innate immune cell trafficking during chronic infection and inflam-mation, such as in tuberculosis (TB), are incompletely understood. During active TB, myeloid cells infiltrate the lung [...]

Published
2013

29. Biomimetic hydroxyapatite nanocrystals are an active carrier for Salmonella bacteriophages

Author

Fulgione, Andrea, Ianniello, Flora, Papaianni, Marina, Contaldi, Felice, Sgamma, Tiziana, Giannini, Cinzia, Pastore, Stella, Velotta, Raffaele, Della Ventura, Bartolomeo, Roveri, Norberto, Lelli, Marco, Capuano, Federico, Capparelli, Rosanna, Fulgione, Andrea, Ianniello, Flora, Papaianni, Marina, Contaldi, Felice, Sgamma, Tiziana, Giannini, Cinzia, Pastore, Stella, Velotta, Raffaele, Della Ventura, Bartolomeo, Roveri, Norberto, Lelli, Marco, Capuano, Federico, and Capparelli, Rosanna

Subjects
bacteriophages, antibiotic resistance, International Journal of Nanomedicine, Salmonella Rissen, biomimetic hydroxyapatite nanocrystals
Abstract

Andrea Fulgione,1,* Flora Ianniello,1,* Marina Papaianni,1 Felice Contaldi,1 Tiziana Sgamma,2 Cinzia Giannini,3 Stella Pastore,3 Raffaele Velotta,4 Bartolomeo Della Ventura,5 Norberto Roveri,6 Marco Lelli,6 Federico Capuano,7 Rosanna Capparelli1 1Department of Agriculture, University of Naples “FedericoII”, Portici, Naples, Italy; 2Biomolecular Technology Group, School of Allied Health Sciences, De Montfort University, Leicester, UK; 3Istituto di Cristallografia, Consiglio Nazionale delle Ricerche, Bari, Italy; 4Department of Physics “Ettore Pancini”, University of Naples “Federico II”, Portici, Naples, Italy; 5Department of Physics, Politecnico di Milano, Milano, Italy; 6Chemical Center Srl, Granarolo dell’Emilia, Bologna, Italy; 7Department of Food Microbiology, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Naples, Italy *These authors contributed equally to this work Purpose: The use of bacteriophages represents a valid alternative to conventional antimicrobial treatments, overcoming the widespread bacterial antibiotic resistance phenomenon. In this work, we evaluated whether biomimetic hydroxyapatite (HA) nanocrystals are able to enhance some properties of bacteriophages. The final goal of this study was to demonstrate that biomimetic HA nanocrystals can be used for bacteriophage delivery in the context of bacterial infections, and contribute – at the same time – to enhance some of the biological properties of the same bacteriophages such as stability, preservation, antimicrobial activity, and so on. Materials and methods: Phage isolation and characterization were carried out by using Mitomycin C and following double-layer agar technique. The biomimetic HA water suspension was synthesized in order to obtain nanocrystals with plate-like morphology and nanometric dimensions. The interaction of phages with the HA was investigated by dynamic light scattering and Zeta potential analyses. The cytotoxicity and intracellular killing activities of the phage–HA complex were evaluated in human hepatocellular carcinoma HepG2 cells. The bacterial inhibition capacity of the complex was assessed on chicken minced meat samples infected with Salmonella Rissen. Results: Our data highlighted that the biomimetic HA nanocrystal–bacteriophage complex was more stable and more effective than phages alone in all tested experimental conditions. Conclusion: Our results evidenced the important contribution of biomimetic HA nanocrystals: they act as an excellent carrier for bacteriophage delivery and enhance its biological characteristics. This study confirmed the significant role of the mineral HA when it is complexed with biological entities like bacteriophages, as it has been shown for molecules such as lactoferrin. Keywords: biomimetic hydroxyapatite nanocrystals, Salmonella Rissen, bacteriophages, antibiotic resistance

Published
2019

30. Production and Characterization of Medium-Sized and Short Antioxidant Peptides from Soy Flour-Simulated Gastrointestinal Hydrolysate

Author

Cavaliere, Chiara, primary, Montone, Angela Michela Immacolata, additional, Aita, Sara Elsa, additional, Capparelli, Rosanna, additional, Cerrato, Andrea, additional, Cuomo, Paola, additional, Laganà, Aldo, additional, Montone, Carmela Maria, additional, Piovesana, Susy, additional, and Capriotti, Anna Laura, additional

Published
2021
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37. The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm

Author

Papaianni, Marina, primary, Ricciardelli, Annarita, additional, Casillo, Angela, additional, Corsaro, Maria M., additional, Borbone, Fabio, additional, Della Ventura, Bartolomeo, additional, Velotta, Raffaele, additional, Fulgione, Andrea, additional, Woo, Sheridan L., additional, Tutino, Maria L., additional, Parrilli, Ermenegilda, additional, and Capparelli, Rosanna, additional

Published
2020
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42. Plant Dynamic Metabolic Response to Bacteriophage Treatment After Xanthomonas campestris pv. campestris Infection

Author

Papaianni, Marina, primary, Paris, Debora, additional, Woo, Sheridan L., additional, Fulgione, Andrea, additional, Rigano, Maria Manuela, additional, Parrilli, Ermenegilda, additional, Tutino, Maria L., additional, Marra, Roberta, additional, Manganiello, Gelsomina, additional, Casillo, Angela, additional, Limone, Antonio, additional, Zoina, Astolfo, additional, Motta, Andrea, additional, Lorito, Matteo, additional, and Capparelli, Rosanna, additional

Published
2020
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50. New perspectives for natural antimicrobial peptides: application as antinflammatory drugs in a murine model

Author

Capparelli Rosanna, De Chiara Francesco, Nocerino Nunzia, Montella Rosa, Iannaccone Marco, Fulgione Andrea, Romanelli Alessandra, Avitabile Concetta, Blaiotta Giuseppe, and Capuano Federico

Subjects
Immunologic diseases. Allergy, RC581-607
Abstract

Abstract Background Antimicrobial peptides (AMPs) are an ancient group of defense molecules. AMPs are widely distributed in nature (being present in mammals, birds, amphibians, insects, plants, and microorganisms). They display bactericidal as well as immunomodulatory properties. The aim of this study was to investigate the antimicrobial and anti-inflammatory activities of a combination of two AMPs (temporin B and the royal jellein I) against Staphylococcus epidermidis. Results The temporin B (TB-KK) and the royal jelleins I, II, III chemically modified at the C terminal (RJI-C, RJII-C, RJIII-C), were tested for their activity against 10 different Staphylococcus epidermidis strains, alone and in combination. Of the three royal jelleins, RJI-C showed the highest activity. Moreover, the combination of RJI-C and TB-KK (MIX) displayed synergistic activity. In vitro, the MIX displayed low hemolytic activity, no NO2- production and the ability to curb the synthesis of the pro-inflammatory cytokines TNF-α and IFN-γ to the same extent as acetylsalicylic acid. In vivo, the MIX sterilized mice infected with Staphylococcus epidermidis in eleven days and inhibited the expression of genes encoding the prostaglandin-endoperoxide synthase 2 (COX-2) and CD64, two important parameters of inflammation. Conclusion The study shows that the MIX – a combination of two naturally occurring peptides - displays both antimicrobial and anti-inflammatory activities.

Published
2012
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