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This investigation sought to comprehensively evaluate the protective properties of Milk Thistle seeds against Supracideinduced oxidative stress in the Marbled Spinefoot fish. Liquid chromatography--mass spectrometry (LC--MS) was employed to identify metabolites present in Silybum marianum seeds. The LC50 value of organophosphorous insecticide was determined as 7.5 μg L-1 through a 24-hour bath exposure. The experimental setup involved a 14-day dietary supplementation of S. marianum seeds followed by exposure to the determined Supracide LC50. Various physiological parameters, including lipid degradation, Alanine aminotransferase (ALT), Lactate dehydrogenase (LDH), and Total cholesterol (T.ch) levels, were measured to assess fish health and stress. Additionally, histopathological examinations were conducted on the hepatopancreas tissues of both normal and stressed fish. The obtained results indicated significant increases (P<0.05) in serum ALT and LDH activities in the group of fish exposed to the toxin. Moreover, the levels of lipid peroxidation products in hepatopancreas homogenates were significantly higher (P<0.05) in the exposed fish group, except for total cholesterol, which exhibited a significant decrease. Histological analysis further revealed notable tissue modifications in the examined organs, while the control group of fish exhibited normal appearance. Taken together, these findings suggest that Milk thistle seeds may possess protective effects against Supracide-induced oxidative stress in challenged S. rivulatus fish. As a result, this study provides valuable insights into potential alternative ameliorating agents for addressing aquaculture contamination caused by organophosphate insecticides. [ABSTRACT FROM AUTHOR]

According to their nutritional value, their ability to adapt to the various environmental conditions, and their versatility, cereals are among the most cultivated plants in the world. However, the ongoing climate changes subject crops to important environmental stress that for some varieties leads to high production losses. Therefore, the selection of species and varieties that are more versatile and adaptable to different environmental conditions can be important. However, the characteristics of some cereals are not completely known; this is a priority before aiming to improve their cultivation. The aim of this study is to characterize select species that are potentially suitable for local environmental conditions and that possess nutritional value. The elemental composition was assessed in different cereal species grown following intensive and organic agriculture practices. Six species were grown for this study with techniques of intensive agriculture: Triticum monococcum L., Triticum dicoccum L., Triticum aestivum L., variety Verna, Triticum durum Desf., variety Senatore Cappelli, Triticum durum Desf., variety Claudio, and Avena strigosa Schreb.; four of these were also grown following organic procedures: Triticum monococcum L., Triticum dicoccum L., Triticum aestivum L., variety Verna, and Triticum durum Desf., variety Senatore Cappelli. The study considered twenty elements, including major nutrients (Ca, K, Mg, P, and S), seven micronutrients (B, Cu, Fe, Mn, Mo, Se, and Zn), and trace elements with toxic properties (Al, Ba, Cd, Cr, Na, Rb, Sc, and Sr) that can be accumulated at the seed level. The results highlight the differences in the element concentrations in the cereal seeds in relation to the genus and species; the highest concentrations of the major nutrients appeared in T. monococcum; the concentrations were 6.9, 2.09, 7.2, and 2.9 mg/g for K, Mg, P, and S, respectively. The highest concentrations of certain micronutrients, B, Ca, Mo, and Se (16, 785, 3.69, and 0.34 μg/g), were in A. strigosa. There is also evidence that the element content can be affected by the adopted cultivation procedure; however, the effects of the growing procedure can be significantly different when different species are considered. T. monococcum, grown by an organic procedure, presented lower concentrations of the major nutrients, while it demonstrated a modest increase in the micronutrients in the T. durum variety organic S. Cappelli, and the production procedure did not affect the elemental composition of the T. aestivum variety Verna. The survey also highlights that the studied species and the growing procedure affected the capacity to accumulate and translocate trace hazardous elements for human health at the seed level. [ABSTRACT FROM AUTHOR]

Agricultural diversification and high-quality products deriving from sustainable crops such as hemp can represent a solution to revitalize marginal areas and reverse land abandonment. This study aimed at comparing four different hemp cultivars (Carmagnola Selezionata, "CS"; Futura 75, "FUT"; Felina 32, "FEL"; Secuieni Jubileu, "JUB") to provide information to select the best suited cultivar for cultivation in mountain marginal areas and for specific end-use applications. Hemp cultivars were cultivated in a single experimental field to compare their ecological and agronomic behavior (duration of life cycle phases, plant size and biomass allocation, and plant resource-use strategies). Through metabolomic analysis of both vegetative and reproductive parts of the plants we tested the presence of substances of nutraceutical interest and traced seed nutritional profile. The four cultivars had different ecological and agronomic behavior, and nutritional profile. We found several compounds with potential pharmaceutical and nutraceutical values in all parts of the plant (leaves, inflorescences, and stems). JUB resulted the most suitable for seed production while CS showed the highest content of bioactive compounds in flowers and leaves. FUT, showed the best suitability for multi-purpose cultivation, while FEL seemed to be not appropriate for the cultivation in mountain area. The multi-disciplinary approach we adopted was effective in distinguish across hemp cultivars and provided information to farmers for the selection of the best hemp cultivar to select. Hemp had a high potential for cultivation in marginal lands, demonstrating to be an economic resource due to its multi-purpose use and to the possibility to generate high-added values products. Our results could serve as a stimulus for the reintroduction of this culture in the study area and in other similar environments. [ABSTRACT FROM AUTHOR]

Even though vehicles are equipped with catalytic converters that decrease NO(sub x), CO, and hydrocarbons, there is substantial risk of contamination from elements in catalytic converters themselves. The study looked for platinum, palladium, and rhodium by testing and comparing Greenland snow from 7000 years ago and Greenland snow from the 1990s. Greenland snow from the 1990s has between approximately 40 to 120 times the levels of catalytic converter particles than the snow from 7000 years ago, suggesting that there is widespread tropospheric pollution in the Northern Hemisphere.

The performance of a double-focusing inductive coupled plasma mass spectrometer equipped with a microconcentric nebulizer was investigated for the direct simultaneous determination of Rh, Pd, and Pt in less than 1 mL of melted snow and ice samples originating from remote sites. Ultraclean procedures were adopted in the laboratories and during the pretreatment steps, to avoid possible contamination problems. Spectroscopic and nonspectroscopic interferences affecting the determination of Rh, Pd, and Pt were carefully considered. Detection limits of 0.02, 0.08, and 0.008 pg g(super -1) for Rh, Pd, and Pt, respectively, were obtained using the following isotopes: (super 103) Rh, (super 106) Pd, and (super 195) Pt. Repeatability of measurements, as RSD, was 27, 28, and 29%, for Ph, Pd, and Pt, respectively. The new method was applied to the analysis of samples coming from Greenland, Antarctica, and the Alps in order to assess the past natural background concentrations and to determine the present level of these polluting substances. The extremely low detection limits allowed the direct analysis of all samples except for two Greenland ice core sections dating from 7260 and 7760 years ago for which a preconcentration step was necessary. Concentration ranges for all snow samples were (in pg g(super 1) as follows: Rh (0.0005-0.39), Pd (0.01-16.9), and Pt (0.008-2.7). The lowest concentrations were measured in the enriched Greenland ice samples