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The swift recuperation of communities following natural hazards heavily relies on the efficiency of transportation systems, facilitating the timely delivery of vital resources and manpower to reconstruction sites. This paper delves into the pivotal role of transportation systems in aiding the recovery of built environments, proposing an evaluative metric that correlates transportation capacity with the speed of post-earthquake recovery. Focusing on optimizing urban population capacity in the aftermath of earthquakes, the study comprehensively examines the impact of pre-earthquake measures such as enhancing building or bridge seismic performance on post-earthquake urban population capacity. The methodology is demonstrated through an analysis of Beijing’s transportation system, elucidating how enhancements to transportation infrastructure fortify the resilience of built environments. Additionally, the concept of a resource supply rate is introduced to gauge the level of logistical support available after an earthquake. This rate tends to decrease when transportation damage is significant or when the demands for repairs overwhelm available resources, indicating a need for retrofitting. Through sensitivity analysis, this study explores how investments in the built environment or logistical systems can increase the resource supply rate, thereby contributing to more resilient urban areas in the face of seismic challenges.

The time-dependent resilience of an in-service aging structure provides quantitative measure of the structural ability to prepare for, adapt to, withstand and recover from disruptive events. Resilience models have been proposed in the literature to evaluate the resilience of aging structures subjected to discrete load processes, which are, however, not applicable to handle resilience problems considering continuous load processes. In this paper, a new method is developed to evaluate the time-dependent resilience of aging structures subjected to a continuous load process. The proposed method serves as the complement of the existing resilience models addressing discrete load processes, and takes into account the aging effects of the structural resistance/capacity and the nonstationarity in loads as a result of climate change. A structure suffers from a damage state upon the occurrence of an upcrossing of the load effect with respect to the resistance/capacity, leading to the reduction of the performance function, followed by a recovery process that restores the performance. The proposed method enables the time-dependent resilience to be evaluated via a closed form solution. It is also revealed that, the proposed resilience model takes an extended form of the existing formula for upcrossing-based time-dependent reliability, thus establishing a unified framework for the two quantities. The applicability of the proposed method is demonstrated through examining the time-dependent resilience of a residential building subjected to wind load. The effects of key factors on resilience, including the nonstationarity and correlation structure of the load process, as well as the resistance/capacity deterioration scenario, are investigated through an example. In particular, the structural resilience would be overestimated if ignoring the potential impacts of climate change, which is a relatively non-conservative evaluation.

Background Congenital absence of the pericardium (CAP) is a rare cardiac abnormality. As pericardial defects are usually asymptomatic, most cases are diagnosed during surgery or on autopsy. The patient in this case was found to have CAP during thoracoscope. Case We present the unusual case of a 69-year-old patient with CAP who experienced sudden ventricular arrhythmia and developed ventricular fibrillation during left upper lobectomy. Surgical operations, the lateral decubitus position, and other external stimuli may be important risk factors for ventricular fibrillation. The patient regained sinus rhythm soon after intrathoracic cardiac compression and pharmacological treatment, including lidocaine spray (2%, 10 ml) administered to the heart surface. The surgery was then completed without any additional instances of ventricular arrhythmia. Conclusions Patients with CAP are more susceptible to cardiac-related adverse events during thoracotomy or thoracoscopy. Treatment of ventricular arrhythmias that occur during lung resection in patients with CAP should be emphasized.

Abstract The lining structures of tunnels are typically constructed using sprayed or cast concrete materials, and their performance and quality during tunnel excavation and blasting are crucial for the stability and safety of tunnels. Therefore, the safe distance between the lining structure and blasting source should be determined to avoid concrete damage caused by blasting vibrations. In this study, taking the subway tunnel of Danshan Station in Qingdao as an example, the JH-2 model is introduced as the constitutive model of the tunnel blasting simulation, and the JH-2 model parameters of the local surrounding rock are obtained by experiments, and finally the numerical simulation and theoretical verification are carried out to study the safety distance of shotcrete under various safety judgment standards. The results indicate that the JH-2 model can effectively simulate the propagation of stress waves under different media conditions, and the closer the strength parameters and pressure constant of the lining structure are to those of the surrounding rock, the safer the concrete–rock bonding interface. During tunnel blasting construction using the ring blasting method, the peak particle velocity (PPV) of the lining structure increases with an increase in the arch angle. Based on the numerical simulation results, we recommend that concrete lining be constructed at a distance of at least 62 m from the blasting source to avoid damage caused by vibrations. The effect of concrete tensile failure caused by longitudinal stress is much smaller than the damage to the bonding interface caused by the PPV and can be neglected.

Aiming at the shortcomings of a traditional centralized control in an active distribution network (AND), this paper proposes a leader-follower distributed group cooperative control strategy to realize multiple operation and control tasks for an ADN. The distributed information exchange protocols of the distributed generation (DG) group devoted to node voltage regulation or exchange power control are developed using a DG power utilization ratio as the consensus variable. On these bases, this study further investigates the leader optimal selection method for a DG group to improve the response speed of the distributed control system. Furthermore, a single or multiple leader selection model is established to minimize the constraints of the one-step convergence factor and the number of leaders to improve the response speed of the distributed control system. The simulation results of the IEEE 33 bus standard test system show the effectiveness of the proposed distributed control strategy. In addition, the response speed of a DG control group can be improved effectively when the single or multiple leaders are selected optimally.

The concrete used in the main structures of subway stations has a high degree of constraint. Consequently, temperature changes and shrinkage during construction frequently lead to significant constraint stress, which can result in structural cracking. Therefore, cement with low hydration heat is commonly used in engineering to reduce the temperature of concrete during its age. Aiming at the problem of hydration and heat release caused by concrete construction, based on the principles of concrete hydration heat release and a numerical analysis method, an optimized semi-adiabatic temperature rise test method has been introduced to investigate concrete temperature rise characteristics with different mineral admixtures. The following conclusions were obtained: The effect of reducing the heat of hydration is related to the content and material properties of different mineral admixtures, but not the type of mineral admixture to be incorporated. The temperature rise performance of four common mineral admixtures is as follows: ① total cooling capacity: limestone powder > slag, fly ash > metakaolin; ② early heat generation rate: metakaolin > slag > fly ash > limestone powder; ③ heat reduction rate in the middle and late periods: metakaolin > limestone powder > fly ash > slag.

Abstract Resilience assessment of civil structures and infrastructure systems plays a vital role in quantitatively measuring the object's ability to withstand and recover from disruptive events (e.g., natural hazards). The performance of the object (structure or system) may degrade with time, and the external loads often display nonstationary characteristics with time‐varying occurrence rate and/or magnitude. In this context, the resilience would be dependent on the duration of the reference period of interest, known as time‐dependent resilience. This article investigates the effect of recovery profile dependency on the time‐dependent resilience of structures and systems. The term “recovery profile dependency” refers to the association of the post‐hazard recovery processes in the aftermath of different load events within the object's service life, and is reflected through the following three aspects: (1) the temporal correlation between the remaining functionalities associated with different load events; (2) the load–recovery interaction (i.e., the scenario that a load event occurs before the full recovery of the degraded functionality due to the previous load event), and (3) the resource allocation strategy in the presence of limited resources, which affects the expeditiousness of each recovery process. In particular, for (2), the concept of average ratio of interaction is proposed to measure the frequency of load‐recovery interaction. The evaluation of time‐dependent resilience is demonstrated through two examples. It is shown that, the time‐dependent resilience is sensitive to the impact of load–recovery interaction and the selection of resource allocation strategy, but is insensitive to the temporal correlation of the remaining functionalities.

IntroductionTo investigate the therapeutic effect of clitoris exposure + episioplasty + dermabrasion + platelet-rich plasma (PRP) injection + chemexfoliation on vulvar lichen sclerosus (VLS).MethodsTwenty children with VLS (under 14 years old) at our hospital from July 2020 to November 2022 were enrolled and treated with clitoris exposure + episioplasty + dermabrasion + PRP injection + chemexfoliation. Additionally, symptomatic changes and improvements in signs were recorded.ResultsSignificant therapeutic effects were achieved in all children enrolled in this study. The Cattanco score was 8.02 ± 1.22 points before surgery, 2.21 ± 0.70 points 3 months after surgery, and 2.61 ± 0.59 points 6 months after surgery, demonstrating that the score after surgery was significantly lower than that before surgery (p < 0.05). Mild complications (one case of mild vulvar swelling, one case of minor bleeding, and one case of superficial ulcer) were observed in three children after surgery, with an overall complication incidence of 15%; all complications were improved after the intervention, and no severe adverse reactions were observed. Recurrence was observed in one child (5%) 6 months after surgery.ConclusionClitoris exposure + episioplasty + dermabrasion + PRP injection + chemexfoliation is an effective approach for the treatment of VLS.Systematic Review Registrationhttps://www.chictr.org.cn/searchproj.html, identifier: ChiCTR2100054787.

Background To satisfy the pharmaceutical care needs of a growing number of patients seeking healthcare in primary care, it is increasingly important to integrate family doctor teams as providers of such care services, while the way bringing the strength of the teams into full play needs to be further explored. Objective To understand the needs of pharmaceutical care services in the community of Shanghai, providing a reference for improving the way of integrating community pharmacists into a family doctor team for delivering such services. Methods This study was conducted from September to December in 2020. Multistage sampling was used to select 349 elderly patients with chronic diseases in the community and 855 main members of family doctor teams〔general practitioners (n=481) , nurses (n=234) , and public health doctors (n=140) 〕 for questionnaire survey. The questionnaire for the patients mainly includes demographics, chronic condition, use of medications, needs and utilization of community-based pharmaceutical care. And the questionnaire for family doctor team members mainly contains demographics, understanding of community-based pharmaceutical care, and pharmaceutical services preferred to be involved in as a provider. Results According to the survey results, the prevalence of three or more chronic diseases, and needing to take medications two or more times a day was 35.8% (125/349) , and 87.4% (305/349) , respectively. Among medication management services, "medication re-prescribing and follow-up" was the most needed〔14.9% (52/349) 〕, and "medication follow-up" was the most used〔14.0% (49/349) 〕. Among medication education services, "universal education on medication cautions for chronic conditions" was the most needed〔70.8% (247/349) 〕, and "healthy lifestyle promotion" was the most used〔9.4% (33/349) 〕. Among family doctor team members, 440 (91.5%) general practitioners, 202 (86.3%) nurses and 112 (80.0%) public health doctors expressed that they had an understanding of pharmaceutical care. In terms of the level of trust in pharmacists, the self-reported level of 302 (62.8%) general practitioners, 183 (78.2%) nurses, and 103 (73.6%) public health doctors was high. The number of general practitioners, nurses, and public health doctors wanting to be involved in "assisting physicians in providing medication selection and use services" was 359 (74.6%) , 112 (47.9%) , and 81 (57.8%) , respectively. The number of general practitioners, nurses, and public health doctors wanting to be involved in "providing nurses with guidance on medication use and management" was 190 (39.5%) , 153 (65.4%) and 57 (40.7%) , respectively. Conclusion Elderly patients with chronic diseases were in urgent need of medication management services, and more medication education services. However, the strength of pharmaceutical professional workforce in the community was weak, and family doctor team members had limited understanding of community-based pharmaceutical services. To efficiently provide residents with such services, it is suggested to train family doctor team members with relevant trainings by pharmaceutical professionals to improve their understanding level and service capability regarding pharmaceutical services at the individual and team levels.

Background Most of the elderly are prone to more than two chronic diseases and polypharmacy. Community health institutions are the key to ensuring rational drug use for in this population. Given many problems existing in the delivery of community pharmaceutical services, how to make better use of existing resources to improve the level of community pharmaceutical services remains to be further explored. Objective To understand the status of community pharmaceutical services implemented in Shanghai, providing a reference for improving community pharmaceutical services. Methods We conducted a survey in Shanghai with 235 pharmaceutical professionals selected by use of phased sampling from September to December 2020 with a questionnaire named as "Community Pharmacists Questionnaire" developed by us based on the results of previous literature review and on-site visits. In November 2020, we conducted a focus group interview with 82 stakeholders (including one pharmaceutical professional, one general practitioner and one community administrator selected from each community, two or three administrators selected from the health commission of each district) in Shanghai guided by an outline named as "Rational Drug Use for the Elderly" developed by us based on the results of previous literature review and on-site visits. The allocation of community pharmaceutical care resources, implementation and management of community pharmaceutical care involved in the interview were extracted as supplements to the survey results. Results Among the 235 community pharmacists surveyed, the prevalence of providing prescription dispensing, prescription review, pharmaceutical drive-thru window services or outpatient consultation and guidance, and prescription comment services was 94.0% (221/235) , 82.6% (194/235) , 64.3% (151/235) , and 51.9% (122/235) , respectively. Among these services, prescription dispensing and prescription review were ranked as the top two in terms of average comprehensive score, indicating that implementing these two services required longer working hours of community pharmacists. In terms of the delivery methods of community pharmaceutical services, community pharmacists mainly used consultation windows〔226 cases (96.2%) 〕, pharmaceutical clinics〔119 cases (50.6%) 〕 and publicity lectures〔109 cases (46.4%) 〕. Only 53 (22.6%) participated in the family doctor team, among whom 22 (41.5%) had the qualification of clinical pharmacist; 47 (88.7%) mainly provided public education on rational drug use, and 45 (84.9%) mainly provided medication guidance and consultation services for patients. In terms of training, 180 cases (76.6%) participated in a training in the past one year, while other 55 (23.4%) cases did not. The number of trainings for most of cases was one or two times〔105 (58.3%) 〕. The way of trainings for most of cases was continuing education〔155 (86.1%) 〕. Both the questionnaire survey and interview results revealed that the main problems existing in the provision of community pharmaceutical services were heavy workload of dispensing/prescription review, lack of clinical knowledge patients' insufficient trust in pharmacists, and lack of performance incentives. Conclusion To meet the pharmaceutical needs of elderly patients with multiple chronic conditions, actions need to be taken to reduce the heavy workload of delivering traditional services in pharmacists, strengthen the clinical training of in-service pharmacists, and encourage pharmacists to join the family doctor team. Through providing pharmaceutical consultations and trainings for other family doctor team members by pharmacists, the scope of influence of pharmaceutical services will be expanded, which contributes to the ensuring of rational drug use.

Engineering structures are often subjected to the influences of performance deterioration and multiple hazards during their service lives, and consequently may suffer from damage/failure as a result of external loads. Structural reliability and resilience assessment is a powerful tool for quantifying the structural ability to withstand these environmental or operational attacks. This paper proposes new formulas for structural time-dependent reliability and resilience analyses in the presence of multiple hazards, which are functions of the duration of the reference period of interest. The joint impacts of nonstationarities in multiple hazards due to a changing environment, as well as the deterioration of structural performance, are explicitly incorporated. The correlation between the structural resistances/capacities associated with different hazard types is modeled by employing a copula function. It is observed that, under the context of multiple hazards and aging effects, the time-dependent resilience takes a generalized form of time-dependent reliability. The proposed formulas can be used to guide the adaptive design of structures, where adaptive strategies are identified across a range of possible future service conditions. An example is presented to demonstrate the applicability of the proposed method for structural reliability and resilience analyses.

Abstract Bone metastasis is one of the most serious complications in lung cancer patients. MicroRNAs (miRNAs) play important roles in tumour development, progression and metastasis. A previous study showed that miR-106a is highly expressed in the tissues of lung adenocarcinoma with bone metastasis, but its mechanism remains unclear. In this study, we showed that miR-106a expression is dramatically increased in lung cancer patients with bone metastasis (BM) by immunohistochemical analysis. MiR-106a promoted A549 and SPC-A1 cell proliferation, migration and invasion in vitro. The results of bioluminescence imaging (BLI), micro-CT and X-ray demonstrated that miR-106a promoted bone metastasis of lung adenocarcinoma in vivo. Mechanistic investigations revealed that miR-106a upregulation promoted metastasis by targeting tumour protein 53-induced nuclear protein 1 (TP53INP1)-mediated metastatic progression, including cell migration, autophagy-dependent death and epithelial–mesenchymal transition (EMT). Notably, autophagy partially attenuated the effects of miR-106a on promoting bone metastasis in lung adenocarcinoma. These findings demonstrated that restoring the expression of TP53INP1 by silencing miR-106a may be a novel therapeutic strategy for bone metastatic in lung adenocarcinoma.

Abstract This study aimed to construct a widely accepted prognostic nomogram in Chinese high-grade osteosarcoma (HOS) patients aged ≤ 30 years to provide insight into predicting 5-year overall survival (OS). Data from 503 consecutive HOS patients at our centre between 12/2012 and 05/2019 were retrospectively collected. Eighty-four clinical features and routine laboratory haematological and biochemical testing indicators of each patient at the time of diagnosis were collected. A prognostic nomogram model for predicting OS was constructed based on the Cox proportional hazards model. The performance was assessed by the concordance index (C-index), receiver operating characteristic curve and calibration curve. The utility was evaluated by decision curve analysis. The 5-year OS was 52.1% and 2.6% for the nonmetastatic and metastatic patients, respectively. The nomogram included nine important variables based on a multivariate analysis: tumour stage, surgical type, metastasis, preoperative neoadjuvant chemotherapy cycle, postoperative metastasis time, mean corpuscular volume, tumour-specific growth factor, gamma-glutamyl transferase and creatinine. The calibration curve showed that the nomogram was able to predict 5-year OS accurately. The C-index of the nomogram for OS prediction was 0.795 (range, 0.703–0.887). Moreover, the decision curve analysis curve also demonstrated the clinical benefit of this model. The nomogram provides an individualized risk estimate of the 5-year OS in patients with HOS aged ≤ 30 years in a Chinese population-based cohort.

In this study, one strain of Beauveria caledonica was isolated from wild fruiting bodies collected from Guizhou Province, China, and its species identification, biological characteristics, domestication, and cultivation methods were studied along with polysaccharide and adenosine content analysis. The mycelia were identified by ITS sequencing, and the fruiting bodies of B. caledonica were domestically cultivated for the first time using wheat and rice as basic cultivation media. The carbon sources, nitrogen sources, cultivation temperatures, and pH for mycelial growth were optimized through single-factor experiments and response surface methodology (RSM) experiments. The polysaccharide content was detected by the phenol–sulfuric acid method, and the adenosine content was measured by high-performance liquid chromatography (HPLC). The results confirmed that the identified mycelia were B. caledonica. The optimum medium for solid culture was 25.8 g/L glycerol, 10.9 g/L yeast extract, 1 g/L MgSO4·7H2O, 1 g/L KH2PO4, 10 mg/L vitamin B1, and 20 g/L agar; the optimum pH was 6.5, and the optimum culture temperature was 25 °C. The optimal liquid culture medium was 26.2 g/L glycerol, 11.1 g/L yeast extract, 1 g/L MgSO4·7H2O, 1 g/L KH2PO4, and 10 mg/L vitamin B1; the mycelia grew well at pH 6.6 and 25 °C. The average biological efficiencies of fruiting bodies on wheat and rice as culture media were 1.880% and 2.115%, respectively; the polysaccharide contents of fruiting bodies on the two media were 6.635% and 9.264%, respectively, while the adenosine contents were 0.145% and 0.150%, respectively. This study provides a valuable reference for further artificial cultivation and utilization of B. caledonica by investigating its biological characteristics, cultivation conditions for artificial domestication, and polysaccharide and adenosine contents in cultivated fruiting bodies. [ABSTRACT FROM AUTHOR]

Subway stations are important components of subway system planning and urban rail transit where passengers can board, alight, transfer, or wait. With an increase in urban passenger flow, subway stations must meet greater transfer‐space demands commensurate with growing station structure sizes. In this study, the field‐monitoring and analysis method was adopted to investigate the Huaxing Street Station of the Chengdu Metro Line 19 project and explore the temperature‐variation rule in the process of mass‐concrete pouring in the station floor. Based on an ABAQUS large‐finite‐element calculation programme and secondary development of damage and failure research methods, a mechanical model of mass‐concrete pouring in subway‐station floor construction was established. The pouring temperature effect, damage, and failure mechanism of mass‐concrete pouring in subway stations were comprehensively studied. The main results were as follows: (1) the maximum temperature difference between the upper and middle layers of the floor was dominant and reached a peak within 1–3 d after the completion of pouring, and the temperature difference gradually decreased with a decrease in the hydration‐heat reaction. (2) At 0–10 d, the damage ratio of the floor increased by 1.8%, predominantly in the form of longitudinal damage. Within 10–30 d, the damage and failure ratio of the floor increased by 1.86%, primarily in the form of transverse damage and failure. Within 30–40 d, the temperature of the floor stabilized and gradually approached the ambient temperature. The damage and failure ratio of the floor increased by 0.32% at this time. (3) The lower the ambient temperature, the greater was the damage sustained by the floor at different ages, and the ambient temperature had a significant influence on floor damage. [ABSTRACT FROM AUTHOR]

BackgroundIncreased plaque vulnerability and higher lipid variability are causes of adverse cardiovascular events. Despite a close association between glucose and lipid metabolisms, the influence of elevated glycated hemoglobin A1c (HbA1c) on plaque vulnerability and lipid variability remains unclear.MethodsAmong subjects undergoing percutaneous coronary intervention (PCI) from 2009 through 2019, 366 patients received intravascular optical coherence tomography (OCT) assessment and 4,445 patients underwent the scheduled follow-ups within 1 year after PCI. Vulnerability features of culprit vessels were analyzed by OCT examination, including the assessment of lipid, macrophage, calcium, and minimal fibrous cap thickness (FCT). Visit-to-visit lipid variability was determined by different definitions including standard deviation (SD), coefficient of variation (CV), and variability independent of the mean (VIM). Multivariable linear regression analysis was used to verify the influence of HbA1c on plaque vulnerability features and lipid variability. Exploratory analyses were also performed in non-diabetic patients.ResultsAmong enrolled subjects, the pre-procedure HbA1c was 5.90 ± 1.31%, and the average follow-up HbA1c was 5.98 ± 1.16%. By OCT assessment, multivariable linear regression analyses demonstrated that patients with elevated HbA1c had a thinner minimal FCT (β = −6.985, P = 0.048), greater lipid index (LI) (β = 226.299, P = 0.005), and higher macrophage index (β = 54.526, P = 0.045). Even in non-diabetic patients, elevated HbA1c also linearly decreased minimal FCT (β = −14.011, P = 0.036), increased LI (β = 290.048, P = 0.041) and macrophage index (β = 120.029, P = 0.048). Subsequently, scheduled follow-ups were performed during 1-year following PCI. Multivariable linear regression analyses proved that elevated average follow-up HbA1c levels increased the VIM of lipid profiles, including low-density lipoprotein cholesterol (β = 2.594, P < 0.001), high-density lipoprotein cholesterol (β = 0.461, P = 0.044), non-high-density lipoprotein cholesterol (β = 1.473, P < 0.001), total cholesterol (β = 0.947, P < 0.001), and triglyceride (β = 4.217, P < 0.001). The result was consistent in non-diabetic patients and was verified when SD and CV were used to estimate variability.ConclusionIn patients undergoing elective PCI, elevated HbA1c increases the atherosclerotic plaque vulnerability and the visit-to-visit variability of lipid profiles, which is consistent in non-diabetic patients.

Tissue-resident innate lymphoid cells (ILCs) play a vital role in the frontline defense of various tissues, including the lung. The development of type 2 ILCs (ILC2s) depends on transcription factors such as GATA3, RORα, GFI1, and Bcl11b; however, the factors regulating lung-resident ILC2s remain unclear. Through fate mapping analysis of the paralog transcription factors GFI1 and GFI1B, we show that GFI1 is consistently expressed during the transition from progenitor to mature ILC2s. In contrast, GFI1B expression is limited to specific subsets of bone marrow progenitors and lung-resident ILC progenitors. We found that GFI1B+ lung ILC progenitors represent a multi-lineage subset with tissue-resident characteristics and the potential to form lung-derived ILC subsets and liver-resident ILC1s. Loss of GFI1B in bone marrow progenitors led to the selective loss of lung-resident IL-18R+ ILCs and mature ILC2, subsequently preventing the emergence of effector ILCs that could protect the lung against inflammatory or tumor challenge. Editor's summary: Most innate lymphoid cells (ILCs) develop from lineage-restricted ILC progenitors in the bone marrow, but certain tissues such as the lungs are believed to harbor local ILC progenitor populations. How these local ILC progenitor pools are maintained remains unclear. Using dual reporter mice, Huang et al. mapped expression of the paralog transcription factors GFI1 and GFI1B in ILCs and their progenitors. GFI1B expression alone identified bone marrow and lung ILC progenitors that retained potential to differentiate into multiple ILC lineages. Loss of GFI1B-dependent ILCs impaired type 2 ILC development in the lungs, leading to reduced papain-induced inflammation but impaired protection against establishment of lung tumors. Together, these findings identify GFI1B expression as hallmark of lung-resident ILCs that retain multilineage developmental potential. —Claire Olingy [ABSTRACT FROM AUTHOR]

Fibroblastic reticular cells (FRCs) construct microanatomical niches that support lymph node (LN) homeostasis and coordination of immune responses. Transcription factors regulating the functionality of FRCs remain poorly understood. Here, we investigated the role of the transcription factor SpiB that is expressed in LN FRCs. Conditional ablation of SpiB in FRCs impaired the FRC network in the T‐cell zone of LNs, leading to reduced numbers of FRCs and altered homeostatic functions including reduced CCL21 and interleukin‐7 expression. The size and cellularity of LNs remained intact in the absence of SpiB but the space between the reticular network increased, indicating that although FRCs were reduced in number they stretched to maintain network integrity. Following virus infection, antiviral CD8+ T‐cell responses were impaired, suggesting a role for SpiB expression in FRCs in orchestrating immune responses. Together, our findings reveal a new role for SpiB as an important regulator of FRC functions and immunity in LNs. [ABSTRACT FROM AUTHOR]

The projected increase in sea surface temperature due to climate change is expected to substantially intensify future hurricanes. Wooden light-frame residential buildings are particularly vulnerable to hurricane damage, and their risk is expected to increase due to heightened exposure and intensifying hurricanes. Therefore, adaptation strategies need to be planned to reduce damage to such buildings while considering the impact of climate change on hurricanes. This study investigates the effectiveness of various climate change adaptation strategies for coastal wood-frame single-story residential buildings and demonstrates how these strategies can be planned. The study considers the four Representative Concentration Pathways (RCPs) proposed by the IPCC to investigate the impact of climate change on wind hazard and losses. Additionally, three locations in the coastal United States of varying sizes, exposure, and hurricane hazard levels are considered: Harris County, Texas; Mobile County, Alabama; and Miami-Dade County, Florida. The results show that the increase in wind speeds and losses will be non-linear with time. All considered adaptation strategies decreased losses, with some able to completely counter the increasing losses even under high emission scenarios. Investigating the effectiveness of adaptive measures can guide stakeholders in allocating funds and efforts for hurricane risk management and enhancing community resilience. [ABSTRACT FROM AUTHOR]