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Engineering vasculature networks in physiologically relevant hydrogelsrepresents a challenge in terms of both fabrication, due to the cell–bioinkinteractions, as well as the subsequent hydrogel-device interfacing. Here, anew cell-friendly fabrication strategy is presented to realize perfusablemulti-hydrogel vasculature models supporting co-culture integrated in amicrofluidic chip. The system comprises two different hydrogels to specificallysupport the growth and proliferation of two different cell types selected for thevessel model. First, the channels are printed in a gelatin-based ink bytwo-photon polymerization (2PP) inside the microfluidic device. Then, ahuman lung fibroblast-laden fibrin hydrogel is injected to surround the printednetwork. Finally, human endothelial cells are seeded inside the printedchannels. The printing parameters and fibrin composition are optimized toreduce hydrogel swelling and ensure a stable model that can be perfused withcell media. Fabricating the hydrogel structure in two steps ensures that nocells are exposed to cytotoxic fabrication processes, while still obtaining highfidelity printing. In this work, the possibility to guide the endothelial cellinvasion through the 3D printed scaffold and perfusion of the co-culturemodel for 10 days is successfully demonstrated on a custom-made perfusionsystem.

Despite the technological advances of the last decades, drug development remains a lengthy and costly process with uncertainties still associated with the poor predictive power of the in vitro and animal models. To address this limitation, microphysiological systems have been introduced in an attempt to increase the biological relevance of in vitro devices. One of the current challenges in MPS is the integration of a vasculature network to sustain 3D cultures to closely mimic human physiology. This thesis proposed a new strategy to recreate a more representative vasculature system directly on-chip. As a first step, the 2-photon polymerization was investigated as a 3D printing technique to recreate structures with cell-relevant feature size and resolution. Subsequently, the 2-photon polymerization 3D printing was combined with micromolding to recreate a multi-hydrogel vasculature network integrated on-chip for cell culture. The synergy of the two methods ensured the generation of a high-fidelity multi-hydrogel scaffold for cell co-culture. To preserve the delicate cell culture while still ensuring the sample manipulation for monitoring and analysis, a customized microphysiological system carrier with an integrated heating and perfusion system was also developed. Finally, the possibility of tuning the properties of the 3D-printed hydrogel by controlling the printing parameters was investigated to guide glioblastoma cells to a vascularized compartment. Overall, the thesis not only demonstrated the fabrication versatility of 2 photon polymerization for a vasculature model directly on-chip but also showed the benefits in integrating microphysiological systems on a carrier., FL

The aim of this contribution is to advance the hypothesis of a possible reading under the prism of the melodramatic mode of narratives belonging to the corpus of the generation of Argentinean HIJOS that focus on the theme of intergenerational affective bonds. After a reflection on the characteristics of melodrama and its tradition in Latin America, attention is focused on mapping texts with a testimonial matrix that nevertheless present melodramatic features, to then analyze the specific cases of two novels –Una muchacha muy bella (2013) by Julián López and Magdalufi (2018) by Verónica Sánchez Viamonte– in which the melodramatic mode functions as the articulating matrix.

In the heterogeneous corpus of works published by sons of Argentinean desaparecidos one of the most interesting and original contribution are the texts written by Félix Bruzzone. The aim of this article is to outline the main characteristics of the author’s style and then to analyse his last novel Campo de Mayo (2019). The novel actually originates as another type of text, a performance, so first of all, the peculiarities of this textuality will be identified, in order to reflect on the mechanisms that intervene when a performance is transposed into a novel. It will be demonstrated that the novel keeps many elements of the performance, all linked to the theme of the search for disappeared parents, that ensure that the dynamic corporeal characteristic of the hipotext is kept in its novelisation, in order to create a discourse that through the body of the protagonist displays all the contradictions and paradoxes that this impossible search implies, using de textual image of the moving body to give an account of the complex dynamics of identity definition concerning sons of desaparecidos., Entre el heterogéneo corpus de obras escritas por hijos de desaparecidos argentinos uno de los aportes más interesantes y originales son los textos de Félix Bruzzone. Este artículo se propone como objetivo encuadrar las características principales del estilo del autor para luego analizar su última novela Campo de Mayo (2019). La obra nace en realidad en otra dimensión textual, la de la performance, por esta razón primero se individuaran las peculiaridades de esta textualidad, para luego reflexionar sobre los mecanismos que intervienen en el pasaje de la performance a la novela. Se demostrará que en la novela quedan muchos elementos de la performance, todos vinculados a la temática de la búsqueda de padres desaparecidos, que hacen que la dimensión corporal y dinámica del hipotexto se quede en su ‘novelización’ para armar un discurso que a través de la corporalidad del protagonista pone en escena todas las contradicciones y la paradojas que esta búsqueda imposible implica, sirviéndose de la imagen textual del cuerpo en movimiento para dar cuenta de las complejas dinámicas de definición identitaria que atañen a los hijos de desaparecidos.

Microfluidic chips offer many benefits for cell studies, including an accurate spatial and temporal control over the growth conditions1. Despite the large expansion of microfluidics in biological applications2, there have been only a few developments of devices to simplify microfluidic chip handling and imaging. Here, we present a carrier of well-plate format with integrated cell media recirculation and heating systems to provide a stable environment for the cell culture during the imaging outside the incubator. Moreover, the absence of external tubing reduces the risk of contamination and bubble formation during the carrier transfers and reagent injections enabling long-term experiment monitoring. Our system was validated by repeatedly (day 1, 3, 7 and 10) taking the cultured mouse endothelial cells (bEnd.3) out of the incubator for imaging.

Current bio-interface models often rely upon a porous thin membrane integrated in a microfluidic system to combine the cell proximity with a precise delivery of biochemical and biophysical cues1. However, this strategy still fails to provide the cultured cells with a good mimic of the tissue physiology. Compared to a conventional porous membrane, a natural-sourced hydrogel represents a more suitable candidate to recapitulate the 3D environment of the biological counterpart2. The platform presented in this study includes a 200 µm thick methacrylated gelatin (GelMA) membrane fabricated with 2-photon polymerization3 in-between two microfluidic channels.