Search

Your search keyword '"Cantacessi Cinzia"' showing total 604 results
Start Over Author "Cantacessi Cinzia"
604 results on '"Cantacessi Cinzia"'

5. Major prospects for exploring canine vector borne diseases and novel intervention methods using 'omic technologies

Author

Hofmann Andreas, Campbell Bronwyn E, Cantacessi Cinzia, Gasser Robin B, and Otranto Domenico

Subjects
Infectious and parasitic diseases, RC109-216
Abstract

Abstract Canine vector-borne diseases (CVBDs) are of major socioeconomic importance worldwide. Although many studies have provided insights into CVBDs, there has been limited exploration of fundamental molecular aspects of most pathogens, their vectors, pathogen-host relationships and disease and drug resistance using advanced, 'omic technologies. The aim of the present article is to take a prospective view of the impact that next-generation, 'omics technologies could have, with an emphasis on describing the principles of transcriptomic/genomic sequencing as well as bioinformatic technologies and their implications in both fundamental and applied areas of CVBD research. Tackling key biological questions employing these technologies will provide a 'systems biology' context and could lead to radically new intervention and management strategies against CVBDs.

Published
2011
Full Text
View/download PDF

6. Differences in transcription between free-living and CO2-activated third-stage larvae of Haemonchus contortus

Author

Zhong Weiwei, Zawadzki Jodi L, Presidente Paul JA, Hall Ross S, Jex Aaron R, Young Neil D, Campbell Bronwyn E, Cantacessi Cinzia, Aleman-Meza Boanerges, Loukas Alex, Sternberg Paul W, and Gasser Robin B

Subjects
Biotechnology, TP248.13-248.65, Genetics, QH426-470
Abstract

Abstract Background The disease caused by Haemonchus contortus, a blood-feeding nematode of small ruminants, is of major economic importance worldwide. The infective third-stage larva (L3) of this gastric nematode is enclosed in a cuticle (sheath) and, once ingested with herbage by the host, undergoes an exsheathment process that marks the transition from the free-living (L3) to the parasitic (xL3) stage. This study explored changes in gene transcription associated with this transition and predicted, based on comparative analysis, functional roles for key transcripts in the metabolic pathways linked to larval development. Results Totals of 101,305 (L3) and 105,553 (xL3) expressed sequence tags (ESTs) were determined using 454 sequencing technology, and then assembled and annotated; the most abundant transcripts encoded transthyretin-like, calcium-binding EF-hand, NAD(P)-binding and nucleotide-binding proteins as well as homologues of Ancylostoma-secreted proteins (ASPs). Using an in silico-subtractive analysis, 560 and 685 sequences were shown to be uniquely represented in the L3 and xL3 stages, respectively; the transcripts encoded ribosomal proteins, collagens and elongation factors (in L3), and mainly peptidases and other enzymes of amino acid catabolism (in xL3). Caenorhabditis elegans orthologues of transcripts that were uniquely transcribed in each L3 and xL3 were predicted to interact with a total of 535 other genes, all of which were involved in embryonic development. Conclusion The present study indicated that some key transcriptional alterations taking place during the transition from the L3 to the xL3 stage of H. contortus involve genes predicted to be linked to the development of neuronal tissue (L3 and xL3), formation of the cuticle (L3) and digestion of host haemoglobin (xL3). Future efforts using next-generation sequencing and bioinformatic technologies should provide the efficiency and depth of coverage required for the determination of the complete transcriptomes of different developmental stages and/or tissues of H. contortus as well as the genome of this important parasitic nematode. Such advances should lead to a significantly improved understanding of the molecular biology of H. contortus and, from an applied perspective, to novel methods of intervention.

Published
2010
Full Text
View/download PDF

12. MIxS-SA: a MIxS extension defining the minimum information standard for sequence data from symbiont-associated micro-organisms

Author

Jorge, Fátima, Brealey, Jaelle C., Brindley, Paul J., Buysse, Marie, Cantacessi, Cinzia, Duron, Olivier, Fichorova, Raina, Fitzpatrick, Connor R., Hahn, Megan, Hunter, Christopher, Hervé, Vincent, Knoll, Laura J., Kohl, Kevin D., Lalle, Marco, Lukeš, Julius, Martínez, Joaquín Martínez, Perkins, Susan L., Poulin, Robert, Rosario, Karyna, Schneider, Adam C., Schriml, Lynn M., Thompson, Luke R., Walls, Ramona L., and Dheilly, Nolwenn M.

Published
2022
Full Text
View/download PDF

22. The dynamics of Toxoplasmosis epidemiology in large islands: the role of wild boar (Sus scrofa)

Author

Sini, Maria Francesca, primary, Manconi, Maria, additional, Varcasia, Antonio, additional, Massei, Giovanna, additional, Sandu, Ramona, additional, Mehmood, Naunain, additional, Ahmed, Fahad, additional, Carta, Carlo, additional, Cantacessi, Cinzia, additional, Scarano, Christian, additional, Scala, Antonio, additional, and Tamponi, Claudia, additional

Published
2023
Full Text
View/download PDF

26. Lungworms and gastrointestinal parasites of domestic cats: a European perspective

Author

Giannelli, Alessio, Capelli, Gioia, Joachim, Anja, Hinney, Barbara, Losson, Bertrand, Kirkova, Zvezdelina, René-Martellet, Magalie, Papadopoulos, Elias, Farkas, Róbert, Napoli, Ettore, Brianti, Emanuele, Tamponi, Claudia, Varcasia, Antonio, Margarida Alho, Ana, Madeira de Carvalho, Luís, Cardoso, Luís, Maia, Carla, Mircean, Viorica, Mihalca, Andrei Daniel, Miró, Guadalupe, Schnyder, Manuela, Cantacessi, Cinzia, Colella, Vito, Cavalera, Maria Alfonsa, Latrofa, Maria Stefania, Annoscia, Giada, Knaus, Martin, Halos, Lénaïg, Beugnet, Frederic, and Otranto, Domenico

Published
2017
Full Text
View/download PDF

30. Worms and gut microbes-Best of frenemies

Author

Cantacessi, Cinzia, Cantacessi, Cinzia [0000-0001-6863-2950], and Apollo - University of Cambridge Repository

Subjects
Helminths, Animals, Parasites, Gastrointestinal Microbiome
Abstract

Over millions of years, helminths have shared their host habitat with a myriad of other microorganisms, collectively known as the ‘microbiota’. Of the microbial communities that inhabit host organs and tissues, those residing in the gastrointestinal tract are known to play active roles in several key biological processes, including but not limited to, nutrient processing and absorption, defence against pathogens and, crucially, development and maintenance of innate and adaptive immunity. Over the past decade, the tight association between parasitic worms and the gut microbiota has attracted significant interest, as the concept of ‘host-parasite interactions’ has progressively shifted towards acknowledging the former as ‘holobiont’, i.e. the host in association with its associated mutualistic symbionts. Thus, a deep knowledge of worm-holobiont interactions is essential to better understand worm biology and mechanisms of helminth disease, and ultimately to identify parasite ‘Achille’s heels’ that may represent useful targets of helminth control.

Published
2023
Full Text
View/download PDF

34. Gastrointestinal worms and bacteria: from association to intervention

Author

Rooney, James, Cantacessi, Cinzia, Sotillo, Javier, Cortés, Alba, Rooney, James [0000-0001-9986-0307], Sotillo, Javier [0000-0002-1443-7233], Cortés, Alba [0000-0002-9027-8780], Apollo - University of Cambridge Repository, Instituto de Salud Carlos III, and Cantacessi, Cinzia [0000-0001-6863-2950]

Subjects
postbiotics, Bacteria, Probiotics, Microbiota, Immunology, Postbiotics, Bacterial extracellular vesicles, Gastrointestinal Microbiome, Prebiotics, probiotics, bacterial extracellular vesicles, Bioactive bacterial metabolites, helminth-gut microbiota interactions, bacterial vaccines, Humans, Parasitology, bioactive bacterial metabolites, prebiotics, Bacterial vaccines, Helminth-gut microbiota interactions
Abstract

A plethora of studies, both experimental and epidemiological, have indicated the occurrence of associations between infections by gastrointestinal (GI) helminths and the composition and function of the host gut microbiota. Given the worldwide risk and spread of anthelmintic resistance, particularly for GI parasites of livestock, a better understanding of the mechanisms underpinning the relationships between GI helminths and the gut microbiome, and between the latter and host health, may assist the development of novel microbiome-targeting and other bacteria-based strategies for parasite control. In this article, we review current and prospective methods to manipulate the host gut microbiome, and/or to exploit the immune stimulatory and modulatory properties of gut bacteria (and their products) to counteract the negative impact of GI worm infections; we also discuss the potential applications of these intervention strategies in programmes aimed to aid the fight against helminth diseases of humans and livestock. Javier Sotillo is a Miguel Servet Fellow funded by Instituto de Salud Carlos III (CP17III/00002). Sí

Published
2022

39. Investigation of host-microbe-parasite interactions in an in vitro 3D model of the vertebrate gut

Author

Owens, Roisin, Cantacessi, Cinzia, Moysidou, Chrysanthi, Bryant, Clare, Owens, Roisin [0000-0001-7856-2108], Cantacessi, Cinzia [0000-0001-6863-2950], Moysidou, Chrysanthi-Maria [0000-0001-9809-2764], Bryant, Clare [0000-0002-2924-0038], and Apollo - University of Cambridge Repository

Subjects
helminths, Bacteria, Vertebrates, gut microbiome, Animals, 3D biology, Parasites, host-microbe interactions, in vitro models, Gastrointestinal Microbiome, Host-Parasite Interactions
Abstract

In vitro models of the gut-microbiome axis are in high demand. Conventionally, intestinal monolayers grown on Transwell culture setups are used to test the effects of commensals/pathogens on the integrity of the barrier, both under homeostatic and pathophysiological conditions. While such models remain valuable for deepening our understanding of host-microbe interactions, often they lack key biological components that mediate this intricate crosstalk. Here, we develop a 3D in vitro model of the vertebrate intestinal epithelium, interfaced with immune cells surviving in culture for over three weeks, and demonstrate its applicability to proof-of-concept studies of host-microbe interactions. More specifically, we describe the establishment of stable host-microbe co-cultures and show functional and morphological changes in the intestinal barrier induced by the presence of commensal bacteria. Finally, we provide evidence that our vertebrate 3D gut models can be used as platforms to test host-microbe-parasite interactions. Exposure of gut-immune-bacteria co-cultures to helminth ‘excretory/secretory products’ (ESP) induces in vivo-like up-/down-regulation of certain cytokines. These findings support the robustness of our modular in vitro cell systems for investigating the dynamics of host-microbe crosstalk and pave the way toward new approaches for systems biology studies of pathogens that cannot be maintained in vitro, such as parasitic helminths., The authors wish to acknowledge funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement no. 723951 to RMO). This material is based upon work supported by the Air Force Office of Scientific Research under award number FA8655-20-1-7021 to RMO. The CC laboratory is funded by grants by the Biotechnology and Biological Sciences Research Council, the Isaac Newton Trust and the University of Cambridge. AJN is funded by the Scottish Government: Rural & Environment Science & Analytical Service and DRGP receives support from the Moredun Foundation.

Published
2022

Catalog

Books, media, physical & digital resources
See catalog results