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Introduction: Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease for which certain risk factors are well known: obesity and smoking (in particular). However, the factors associated with more severe conditions, and therefore potential aggravators of the disease, remain a matter of debate. Our study aims to determine the clinical factors associated with severe HS using several severity scores. Methods: The data were obtained via the ERHS questionnaire from patients exclusively recruited at Erasme Hospital in Brussels. The severity of HS was firstly estimated by the Hurley score, and secondly by a metascore, a system combining the iHS4, HS-PGA, SAHS, and DLQI. Univariable and multivariable analyses were performed. Results: Six hundred and forty-seven patients were included in the Hurley analysis, and 456 patients in the metascore analysis. In multivariable analysis, men have a more severe metascore than women (odds ratio [OR] = 1.89, p = 0.022), smoking was associated with a more severe disease according to metascore, especially in mild cases (OR = 0.76, p = 0.043), and an elevated body mass index was associated with having Hurley stage III disease compared to Hurley I or II disease (OR = 1.09, p = 0.001). A significant association is also shown between blood pressure and Hurley stage (OR = 0.97, p = 0.025). Self-reports of nonsteroidal anti-inflammatory drugs aggravating the disease is also a factor associated with greater severity according to the metascore (OR = 0.12, p = 0.008). Finally, several locations of HS lesions were associated with greater severity, in particular the armpits according to the metascore (OR = 0.29, p < 0.001), and the perianal area according to the Hurley score (OR = 0.15, p < 0.001). Conclusion: HS seems to be more severe in men; smoking seems to aggravate mild cases of HS, while increased body mass index plays a major role in the transition from Hurley II to Hurley III. [ABSTRACT FROM AUTHOR]

Macrophages play a pivotal role as host cells for Leishmania parasites, displaying a notable functional adaptability ranging from the proinflammatory, leishmanicidal M1 phenotype to the anti-inflammatory, parasite-permissive M2 phenotype. While macrophages can potentially eradicate amastigotes through appropriate activation, Leishmania employs diverse strategies to thwart this activation and redirect macrophages toward an M2 phenotype, facilitating its survival and replication. Additionally, a competition for iron between the two entities exits, as iron is vital for both and is also implicated in macrophage defensive oxidative mechanisms and modulation of their phenotype. This review explores the intricate interplay between macrophages, Leishmania, and iron. We focus the attention on the potential of iron oxide nanoparticles (IONPs) as a sort of immunotherapy to treat some leishmaniasis forms by reprogramming Leishmania-permissive M2 macrophages into antimicrobial M1 macrophages. Through the specific targeting of iron in macrophages, the use of IONPs emerges as a promising strategy to finely tune the parasite-host interaction, endowing macrophages with an augmented antimicrobial arsenal capable of efficiently eliminating these intrusive microbes. [ABSTRACT FROM AUTHOR]

Extramammary Paget disease (EMPD) is an uncommon adenocarcinoma of apocrine gland-rich areas, presenting significant diagnostic challenges due to its nonspecific clinical appearance and frequent misidentification as benign, inflammatory skin conditions. Traditional diagnostic methods such as biopsy are invasive and uncomfortable, often required repeatedly due to high recurrence rates. Dermoscopy and non-invasive imaging techniques have been used but provide limited diagnostic accuracy due to their constraints in depth penetration and resolution. Recent advancements in imaging technologies, such as line-field confocal optical coherence tomography (LC-OCT), show promise in enhancing diagnostic precision while minimizing invasive procedures. LC-OCT merges high-resolution imaging with deep penetration capabilities, capturing detailed horizontal and vertical skin images akin to histopathology. This study evaluated the diagnostic performance of LC-OCT in detecting EMPD and its recurrence in 17 clinically suspicious anogenital regions, belonging to six patients. Data were collected prospectively at the patient's bedside by an LC-OCT expert with poor training for EMPD, and, then, reviewed retrospectively by an independent LC-OCT expert with adequate training for EMPD and no concerns about time. The prospective examination yielded 64.7% accuracy (11 true results out of 17 total cases), 71.4% sensitivity (10 true positives out of 14 actual positives), and 33.3% specificity (1 true negative out of 3 actual negatives). The retrospective analysis achieved 94.1% accuracy (16 true results out of 17 total cases), 100% sensitivity (14 true positives out of 14 actual positives), and 66.7% specificity (2 true positives out of 3 actual positives), with the only false positive case being a difficult-to-diagnose concomitant presentation of a lichen sclerosus et atrophicus. Despite the need for specialized training, our results suggest that LC-OCT represents a valuable tool for accurately identifying EMPD and improving its management by reducing unnecessary biopsies. Further studies are needed to standardize its clinical application. [ABSTRACT FROM AUTHOR]

Contaminated food-contact surfaces can transfer pathogenic organisms within domestic settings, potentially leading to foodborne illness. The objective of this study was to evaluate the efficacy of an ozonated water handheld spray bottle to eliminate bacteria on food-contact surfaces. Escherichia coli, Listeria innocua, Pseudomonas spp., and Staphylococcus aureus were used to inoculate polypropylene cutting boards and stainless steel knives. A 2-ppm ozonated water spray was applied to the inoculated surfaces and allowed to act for 30 or 60 s. Ozonated water was able to significantly reduce (P < 0.05) generic E. coli on cutting boards (0.7 ± 0.1 log10 CFU/cm²). In addition, L. innocua was significantly reduced (P < 0.05) on polypropylene (0.9 ± 0.2 log10 CFU/cm²) and stainless steel (1.1 ± 0.1 log10 CFU/cm²). There were no significant reductions of Pseudomonas (0.5 ± 0.3 log10 CFU/cm²) or S. aureus (0.2 ± 0.1 log10 CFU/cm²). Significant reductions were only observed after 60-s treatments, and there was no significant effect of surface type on the reductions. Overall, low concentration-ozonated water is moderately effective at reducing nonpathogenic bacteria loosely attached to simulated clean food-contact surfaces. [ABSTRACT FROM AUTHOR]

This article discusses a new dermoscopic criterion for diagnosing basal cell carcinoma (BCC) called "negative maple-leaf-like areas" (NMLLAs). NMLLAs are round, nonpigmented structures with a whitish background and well-defined borders. They are associated with superficial BCC and correspond to non-pigmented tumor nests at the dermal-epidermal junction level. The article also introduces a new technology called line-field confocal optical coherence tomography (LC-OCT), which combines the advantages of reflectance confocal microscopy (RCM) and conventional OCT. LC-OCT allows for a precise correlation of dermoscopic criteria with skin images. The article presents a case study that demonstrates the correlation between NMLLAs seen in dermoscopy and hemispheric BCC lobules observed with LC-OCT. The use of LC-OCT may help clinicians identify NMLLAs, which can be difficult to recognize. Further studies are needed to confirm this hypothesis. [Extracted from the article]

The objective of this study was to evaluate the effectiveness of ozonated water to reduce Salmonella and aerobic plate counts (APCs) on raw chicken wing sections. Samples were inoculated with a cocktail of five poultry-borne Salmonella strains (mean 6.3 ± 0.3 log10 CFU/ml rinsate). The chicken wing sections (51.3 ± 12.4 g) were treated with ozonated water at three concentrations (2.5, 5.0, and 10.0 ppm) and three exposure times (15, 30, and 45 s) by either immersion or direct application (directly from a hose). Tap water was used as control, and the experiments were performed in triplicate with three wings per treatment. Salmonella reductions with ozonated water ranged from 0.3 ± 0.1 to 0.7 ± 0.1 log™ CFU/ml rinsate. APC reductions with ozonated water ranged from 0.3 ± 0.1 to 1.8 ± 0.7 log10 CFU/ml rinsate. Reductions were numerically higher, although not significantly, with ozonated water than with tap water. Ozone concentration had a significant effect (P = 0.003) on Salmonella reductions in immersion treatment. Although Salmonella reductions on chicken wing sections were considered marginal, future work should focus on evaluating longer exposure times and ozone concentrations on the microbiological quality of raw chicken parts, possibly as part of a multihurdle antimicrobial approach. [ABSTRACT FROM AUTHOR]

In recent years, violence by adolescents towards parents is an issue that has become a high profile concern among professionals and researchers. Cottrell (2001) defined parent abuse as 'any act [...], This study aimed to explore the family profile of adolescent-to-parent abuse cases. Concretely, this paper examines whether or not there is a different family profile of parent-abuse offenders compared with other types of offenders and with non-offender adolescents. The sample included 90 adolescents who were clustered into three groups (parent-abuse offenders, other type of offenders, and nonoffender adolescents). Participants completed measures of quality of communication with parents and parents&apos; educational styles. They were also evaluated using a brief interview with questions about family structure. Results indicate offenders who assault their parents have a different family structure and dynamics. Differences were found concerning the type of household, family size, and family incomes. In addition, parent-abuse offenders reported a lower quality of communication with both parents perceiving them as less warm, more rejecting, and less inductive than did the other groups. Keywords Family violence Young offenders Parent abuse * Aggressive behavior * Family profile

Wild lagomorphs including rabbits and hares can act as natural carriers or reservoirs of bacterial and parasitic zoonotic diseases. However, little is known on the epidemiology and potential public health significance of intestinal eukaryotes in wild leporids. We examined faecal samples from European wild rabbits (Oryctolagus cuniculus, n = 438) and Iberian hares (Lepus granatensis, n = 111) collected in the Autonomous Region of Andalusia in southern Spain during 2012–2021. We searched for the presence of DNA from the main intestinal protist and microsporidial pathogens of veterinary and public health concerns using molecular methods (PCR followed by Sanger and next‐generation sequencing). Giardia duodenalis was the most prevalent species found (27.8%, 153/550; 95% CI: 24.1–31.8), followed by Cryptosporidium spp. (1.3%, 7/550; 95% CI: 0.5–2.6), Blastocystis sp. (1.1%, 6/550; 95% CI: 0.4–2.4) and Encephalitozoon intestinalis (0.2%, 1/550; 95% CI: 0.0–10.1). All samples tested negative for Enterocytozoon bieneusi. Sequence analyses revealed the presence of sub‐assemblage BIV (n = 1) within G. duodenalis, and Cryptosporidium cuniculus (n = 6) and Cryptosporidium andersoni (n = 1) within Cryptosporidium. The presence of ruminant‐adapted C. andersoni is indicative of a potential cross‐species transmission event, although a spurious infection (mechanical carriage) cannot be ruled out. Samples assigned to C. cuniculus belonged to the gp60 subtype families Va (n = 3) and Vb (n = 2). The six Blastocystis‐positive samples were identified as ST2 (n = 3) and ST1 + ST2 (n = 3). Our molecular results suggest that wild rabbits and hares were primarily infected by leporid‐adapted species of eukaryotic pathogens. However, the occasional findings of zoonotic G. duodenalis sub‐assemblage BIV, Blastocystis sp. ST1 and ST2, and Encephalitozoon intestinalis could be of public health relevance. [ABSTRACT FROM AUTHOR]