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Background: Canarium species are reported rich of polyphenol, alkaloids, flavones, and saponins compounds. Canarium odontophyllum from Borneo's variety, on the other hand is remaining unclear especially on the antioxidant activity towards radicals. Purpose: To investigate phytochemical composition and antioxidant properties of C. odontophyllum. Methods: Antioxidant properties were evaluated using 2,2-diphenyl-1-picrylhydrazyl-hydrate (DPPH) assay, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) assay, and ferric-reducing antioxidant power (FRAP) assay with different polarity of extraction solvents (methanol, acetone and hexane). The phytochemical composition was determined by Liquid Chromatography Mass Spectrometry Quadrupole Time of Flight (LCMS-QTOF). Results: Acetone showed the highest scavenging activity in the DPPH (0.0307 mg/ml) and FRAP (3.926 mg/ml) assays, while hexane exhibited the highest activity in the ABTS assay (0.119 mg/ml). The LCMS-QTOF chromatogram identified 22 potential compounds. Among these, the major compounds oleamide (10.49 %) and emmotin A (7.23 %) have previously been reported to exhibit antioxidant activity. Other health-beneficial compounds were also found, such as palmitic amide (2.46 %), deoxymiroestrol (6.23 %), and N-Cyclohexanecarbonylpentadecylamine (48.92 %), which are important in preventing metal corrosion, regulating hormones, and distinguishing between N-palmitoylethanolamine-hydrolyzing acid amidase (NPAA) and fatty acid amide hydrolase (FAAH), respectively. Conclusion: The results indicate that C. odontophyllum shows a promising potential an alternative drug in pharmaceutical developments.

Acute exposure of eukaryotic cells to ultraviolet-B (UVB) radiation leads to a number of detrimental effects, one such prominent effect of UVB exposure is increased production of free radicals which can lead to oxidative damage. Although, the human skin is well equipped with endogenous antioxidant defence system, often increased levels of free radicals lead to oxidative damage in skin. Skin inflammation, accelerated skin aging, and formation of wrinkles are all consequences of UVB induced photodamage. Hence, it is posited that supplementation of an exogenous antioxidant derived from natural products could prevent and reduce oxidative damage in skin cells. This study set forth to investigate the antioxidative role of terpenoid rich Canarium odontophyllum Miq. (Dabai) extract on acute UVB-induced photodamage human keratinocyte cells (HaCaT). We first evaluated the antioxidative capacity of increasing concentrations of crude extracts of TRCO Dabai extracts (62.50 µg/mL, 125 µg/mL, 250 µg/mL, and 500 µg/mL) through FRAP assay. We found all the tested TRCO extract exhibited antioxidative capacity in dosage dependent manner. We further investigated the effects of pre-treatment 250 µg/mL and 500 µg/mL TRCO on UVB-induced photodamaged HaCaT cell by measuring oxidative stress markers of lipid peroxide (LPO content), protein carbonyl (PC) content, glutathione peroxidase (GSH-Px) and glutathione-S-transferase (GST) activities. Both 250 µg/mL and 500 µg/mL TRCO extract pre-treated UVB-induced HaCaT cell group exhibited significantly reduced lipid peroxides content and GST activity compared to the positive control (p<0.05). Pre-treatment of 250 µg/mL TRCO extract significant enhanced GSH-Px activity (p<0.05). However, no significant difference in protein carbonyl content could be established across all tested groups. Therefore, our results suggest that TRCO extract can offer protection against oxidative damages caused by UVB exposure, and said protective effects can be attributed by its antioxidant properties. [ABSTRACT FROM AUTHOR]

Dabai fruit is an exotic and seasonal fruit in Sarawak. Among the varieties available, the Song variety was chosen due to better taste and high demand amongst local consumers. This study determined the physical properties of dabai (Song variety) at three different fractions: whole fruit, nut, and kernel. According to the results, whole fruit had the highest values in geometric mean diameter (27.86 mm), volume (12.70 cm³), mass (13.89 g), surface area (2442.60 mm²) and angle of repose (39.06°) when compared to nut and kernel. Bulk density of dabai nut reached the highest with the value of 0.63 gcm-3. Kernel had the highest percentage of porosity (80.50) compared to others. The correlations of physical properties between whole fruit, nut and kernel were further analysed using Principal Component Analysis (PCA). The findings can potentially be useful in the design of handling and processing equipment. [ABSTRACT FROM AUTHOR]

Colorectal cancer is the most common malignant cancer in developing countries. Canarium odontophyllum, also known as "Dabai" or "Borneo Olive" is among the natural plants that can potentially be used as an anticancer agent. This study aims to determine the antiproliferative activities and cytotoxicity effects of acetone extract from C. odontophyllum stem bark against human colorectal cancer cell lines HCT 116 and HT 29. Acetone extract of C. odontophyllum stem bark exerted a significant cytotoxic effect on HCT 116 and HT 29 cells determined by MTT assay at the concentration of 12.5 μg/mL to 200 μg/mL for 24, 48, and 72 hours treatment. It was found that acetone extract of C. odontophyllum stem bark inhibited proliferation of HCT 116 with an IC50 value of 184.93 ±.0 μ g/mL, 61.24 ±.1 μ g/mL, 79.98 ±.029 for 24, 48 and 72 hours respectively. The findings also showed that acetone extract of C. odontophyllum stem bark revealed a lower inhibitory effect against HT-29 with an IC50 value of more than 200 μg/mL for 24, 48 and 72 hours. However, acetone extract of C. odontophyllum stem bark at similar concentrations and time points did not show any cytotoxic effect to normal colorectal fibroblast cell CCD18-Co. In conclusion, the acetone extract of C. odontophyllum stem bark exhibited more sensitivity against HCT 116 than HT 29. Its antiproliferative ability towards HCT 116 and HT 29 cells provides insight that this extract may serve as an anticancer agent against colorectal cancer. [ABSTRACT FROM AUTHOR]

The study evaluated the antioxidant capacity of hexane extract of Canarium odontophyllum Miq. leaf; its fatty acids and terpenoids content; and cytotoxic effects on UVB-induced human keratinocytes (HaCaT). FRAP assay was used to determine antioxidant capacity. GC-MS analysis to identify the fatty acids and terpenoids' in the hexane extract of Canarium odontophyllum Miq. leaf. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was carried out to measure the cytotoxic effects of the extract on UVB-induced human keratinocytes. Serial doses of up to 1000 µg/mL extract were administered before UVB irradiation of the cells. FRAP assay showed the extract was found to exhibit antioxidant activity but no significant difference in ascorbic acid equivalent antioxidant capacity (AEAC) between dose 500 µg/mL (5.00 ± 0.35 AEAC) and 1000 µg/mL (5.70 ± 0.29 AEAC) extract. GC-MS analysis showed the extract contained 88.93% of fatty acids and terpenoids, especially n-hexadecanoic acid, spathulenol, and phytol. MTT assay showed no IC50 value for the tested extract dose on UVB-induced HaCaT. Thus, the results suggest the potential application of hexane extract of C. odontophyllum Miq. leaf in terpenoids' studies. In-depth research and isolation of compounds of interest should be done to develop it as a viable medical phytotherapeutic agent. [ABSTRACT FROM AUTHOR]

In the present work, the influence of geographical location on the fatty acid profiles, antioxidant potential, as well as cytotoxicity of edible dabai fruit fractions (kernel, skin, and pulp) were analyzed. The fatty acid profiles were determined by Gas Chromatography (GC), and the antioxidant activity was quantified with free 2,2-diphenyl-1-picr/ylhdrazyl, while the cytotoxicity was assessed by the brine shrimp lethality test. The results showed that the samples from Sibu, Serian, and Kapit geographical locations had a high content of the saturated fatty acids, ranging from 46.63% to 53.31% in the three fractions. The highest mono-saturated fatty acids (MUFA) content was found in Sibu. Serian and Kapit kernel fractions MUFA, however, ranged from 21.2% to 45.91%. No fatty acid composition was detected in Bentong and Kanowit. The fatty acid composition and DPPH free radical scavenging antioxidant activity of dabai were statistically independent using a multivariate analysis in different localities in Malaysia. The skin fraction had a more appreciable antioxidant potential and toxicity level than the pulp and kernel fractions. The highest antioxidant activity (EC50 198.76 ± 1.06 µg/mL) with an LC50 value of 1387.22 µg/mL was obtained from the Sibu skin fraction. Therefore, the fatty acid composition, antioxidant, as well as cytotoxicity analyses of the extracts from different localities indicated that "geographical location" remarkably influenced fatty acid composition, antioxidant activity, and toxicity. [ABSTRACT FROM AUTHOR]

Background: Malaria is a parasite that is transmitted to human through the bite of a female Anopheles mosquito. Every year human was exposed to the threat of malaria infection. This disease becomes more fatal as these parasites show resistance towards the drug available. Thus, searches for new antimalarial drug are crucial. This study was carried out to evaluate the antimalarial activity in Canarium odontophyllum leaf extracts (methanol, acetone and aqueous) against erythrocytes infected with Plasmodium berghei NK65 using Plasmodium Lactate Dehydrogenase (pLDH) Assay and SYBR green I fluorescence Assay. Method: Three types of solvents were used to extracts Canarium odontophyllum leaf according to increasing polarity index; acetone, methanol and aqueous. These extracts were made into eight-fold serial dilution; concentrations ranging from 0.00001μg / ml as the lowest concentration until 100 μg / ml as the highest concentration and further tested on Plasmodium berghei NK65 infected erythrocytes via ex-vivo. The IC50 (inhibition concentration) 50 readings were taken at the point of 5% parasitemia level and in the synchronization process. Both PLDH assay and SYBR green I fluorescence assay were being carried out simultaneously. Result: The One-way ANOVA showed that there is no significant difference between extracts at 5% parasitemia level, even so methanol was further tested on synchronization process as it showed the lowest reading of IC50 among the three extracts for PLDH assay and SYBR green I fluorescence assay respectively, (IC50 0.00045μg / ml, 0.002 μg / ml). For synchronization stages, the One-way ANOVA result showed there is no significant difference between stages of morphology. However, methanol extracts showed the most potent on schizont, (1.16x10-5 μg / ml ) and young trophozoite, (0.00195 μg / ml ) stages for each method respectively. Conclusions: All three extracts of Canarium odontophyllum leaf were effective on Plasmodium berghei NK65, however methanol showed most promising results and further research on the fractions were required for proper drug development. The Ministry of Higher Learning funded this project, Government of Malaysia, under the Fundamental Research Grant Scheme Code No. FRGS/2/2014/SG05/UKM/02/3.

Defatted dabai (Canarium odontophyllum Miq.) parts are considered good sources of antioxidants. This study aimed to determine the nutritional values and antioxidant properties of dabai cocoa bars and the sensory profile scores of different parts of dabai fruits (oil, oleoresin, and kernel). The second part of this study included the formulation of dark dabai cocoa bars using different percentages (5%, 10%, 15%, 20%, 25%, and 35%) of dabai kernel. The antioxidant activities of the dark dabai and dabai cocoa bars were determined on the basis of DPPH free radical scavenging and thiobarbituric acid reactive species assays. The panelists of the sensory study were asked to score specific sensory attributes using a hedonic scale. Results of the triangle test indicated that the flavor score of the dabai cocoa bar with 40% dabai oleoresin was low and its other sensory scores (aroma and overall acceptability) were also lower than those of the other types of dabai and dark dabai cocoa bars. Furthermore, the dark dabai cocoa bars with 5–35% dabai kernel exhibited an improved formulation, and the cocoa bar containing 20% dabai kernel presented the lowest lipid peroxidation value and higher overall acceptability score than the cocoa bar with 5% dabai kernel. However, the cocoa bar with 5% dabai kernel achieved the highest estimated total phenolic content and DPPH radical scavenging capacity. The β-carotene bleaching inhibition of the dark cocoa (DC) bar was not significantly higher than the cocoa bar with 40% dabai oleoresin. Lastly, the sensory acceptance score of dabai cocoa bars was lower than that of the DC bar due to the crispiness of dabai kernel and the astringent taste of dabai parts. Thus, dabai kernel preparation should be optimized further.

Dabai (Canarium odontophyllum) nutshells have considerable potential to be used as a viable precursor for biomass-based activated carbon. The material was carbonized at a temperature of 700 °C for 2 h, at a heating rate of 10 °C/min under nitrogen gas flow. The char was analyzed the following ways: percentage of yield, percentage of ash content, Fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive x-ray analysis, and Brunauer, Emmett, and Teller surface area. The char had a maximum Brunauer, Emmett, and Teller surface area of 428 m2/g and the nitrogen adsorption-desorption isotherm of the char that was similar to a Type I adsorption isotherm, based on IUPAC classifications. The char also had a high carbon content (up to 93.6%) and a low ash content (3.67%). Therefore, dabai nutshells were found to be a suitable lignocellulosic precursor for the synthesis of activated carbon. [ABSTRACT FROM AUTHOR]

Dabai (Canarium odontophyllum Miq.) is a fruit that is often eaten by first blanching in hot water to make the flesh creamier and softer, before it is served as a snack or side dish. In this study, Dabai fruit was blanched at different temperatures between 60 and 100 °C, with an increment of 10 °C, for up to 10 min, and the kinetics of quality changes (color and texture) were studied. Kinetic models that were assessed for changes of color and texture were zero-order, first-order, and fractional conversion model. The results showed that L parameter had no change throughout the blanching process, while parameters a*, b*, chroma (C), and total color difference (TCD) resulted as significantly increased as the temperature and duration of blanching increased. However, the change of firmness was not significant due to minor changes of firmness as the temperature and time increased. In terms of kinetic models, zero and fractional-conversion order well described the changes of a* parameter; while zero, first and fractional conversion well described parameters b*, C and TCD. Change of firmness did not fit with zero or first-order. All of the kinetic models obeyed the Arrhenius equation. Thus, the fitted kinetic models can be used to design the blanching process of Dabai fruit.

Defatted dabai (Canarium odontophyllum Miq.) parts are considered good sources of antioxidants. This study aimed to determine the nutritional values and antioxidant properties of dabai cocoa bars and the sensory profile scores of different parts of dabai fruits (oil, oleoresin, and kernel). The second part of this study included the formulation of dark dabai cocoa bars using different percentages (5%, 10%, 15%, 20%, 25%, and 35%) of dabai kernel. The antioxidant activities of the dark dabai and dabai cocoa bars were determined on the basis of DPPH free radical scavenging and thiobarbituric acid reactive species assays. The panelists of the sensory study were asked to score specific sensory attributes using a hedonic scale. Results of the triangle test indicated that the flavor score of the dabai cocoa bar with 40% dabai oleoresin was low and its other sensory scores (aroma and overall acceptability) were also lower than those of the other types of dabai and dark dabai cocoa bars. Furthermore, the dark dabai cocoa bars with 5-35% dabai kernel exhibited an improved formulation, and the cocoa bar containing 20% dabai kernel presented the lowest lipid peroxidation value and higher overall acceptability score than the cocoa bar with 5% dabai kernel. However, the cocoa bar with 5% dabai kernel achieved the highest estimated total phenolic content and DPPH radical scavenging capacity. The β-carotene bleaching inhibition of the dark cocoa (DC) bar was not significantly higher than the cocoa bar with 40% dabai oleoresin. Lastly, the sensory acceptance score of dabai cocoa bars was lower than that of the DC bar due to the crispiness of dabai kernel and the astringent taste of dabai parts. Thus, dabai kernel preparation should be optimized further. [ABSTRACT FROM AUTHOR]

The present work was aimed to optimise the supercritical carbon dioxide extraction of dabai oil from dabai pulp and defatted pulp using RSM in comparison with hexane extraction method. Fatty acid profile and antioxidant properties of the extracted dabai oil were also determined. Optimal RSM conditions were 36 MPa pressure, 40°C temperature and 21 min of extraction time. The extracted yields ranged between 14.13% and 15.42%. Supercritical carbon dioxide extracted oils had lower total phenolics, total flavonoids, and antioxidative activities than the hexane-extracted oils. High-performance liquid chromatography results showed that only vanillic, protocatechuic and gallic acids were identified in both hexane and supercritical carbon dioxide extracted oils of defatted dabai pulp. The total saturated fatty acids were higher in hexane-extracted dabai oil as compared to the supercritical carbon dioxide extracted oil, and vice versa for the unsaturated fatty acid. Although supercritical carbon dioxide extracted dabai oils are inferior to the hexane-extracted oils, they are preferred as they are extracted using green technology. [ABSTRACT FROM AUTHOR]

Aims: To evaluate the anti-leptospiral activity of Canarium odontophyllum leaves against Leptospira interrogans serovar Bataviae and Leptospira borgpetersenii serovar Javanica. Methodology and results: The extracts (hexane, acetone, methanol and aqueous) used in this study were tested at concentration ranging from 0.049 mg/mL to 25 mg/mL using broth microdilution method. Percentage inhibition (%) was obtained through OD reading at 400 nm. Only methanol extract was incubated with Leptospira to observe population changes under dark field microscope prior to subjected for DNA damaging studies through gel electrophoresis of genomic DNA. Methanol extract showed the highest percentage inhibition of 66% against L.interrogans serovar Bataviae and 74% against L. borgpetersenii serovar Javanica. The IC50 value of methanol extract was 4.60 mg/mL and 2.25 mg/mL against serovar Bataviae and serovar Javanica, respectively. Both Leptospira culture which was treated with IC50 value of methanol extract showed drastic decrease in population compared to untreated Leptospira for both serovar. There was no DNA damage towards serovar Bataviae. However, serovar Javanica exhibited DNA damage as observed from the presence of DNA fragmentation on the gel electrophoresis. Conclusion, significance and impact of study: These findings confirmed that methanol leaves extract from of Canarium odontophyllum has a potential to control leptospirosis. [ABSTRACT FROM AUTHOR]

Supercritical CO2 (SC-CO2) was used to extract dabai oil. Total phenolic content, total flavonoid content, DPPH radical scavenging assay, and gas chromatography analysis were performed to determine the antioxidant properties of SC-CO2-extracted dabai oil and to compare with hexane-extracted dabai oil. The total phenolic content of SC-CO2-extracted oil was slightly higher than hexane-extracted oil, whereas the total flavonoid content of SC-CO2- extracted dabai oil was four times lower than the hexane-extracted oil. The percentage of radical scavenging activity for SC-CO2-extracted oil was 0.71%, but the hexane-extracted oil had a higher radical scavenging activity of 3%. Analysed using gas chromatography, SC-CO2-extracted oil had lower total saturated fatty acids than the hexane-extracted oil. [ABSTRACT FROM AUTHOR]

Type 1 diabetes mellitus is a chronic disease characterized by lack of insulin production. Immune mechanisms are implicated in the pathogenesis of Type 1 diabetes. Canarium odontophyllum (CO) fruits and leaves have been shown to possess high antioxidant activity. This study was conducted to evaluate the effects of CO leaves aqueous extract on the blood glucose and T lymphocyte population in the spleen of streptozotocin (STZ)-induced diabetic rats. Nineteen male Sprague–Dawley rats were randomly divided into three groups: normal, diabetic control and CO treated diabetic groups. Diabetes was induced by a single intraperitoneal injection of 65 mg STZ/kg body weight. The extract of CO leaves was administered orally by force feeding daily at the dose of 300 mg/kg for 28 days. The rats were sacrificed at the end of the study and the spleen was harvested for flow cytometry analysis. The results showed a significant decrease in body weight of diabetic and CO treated diabetic groups compared with the normal group ( p < 0.05). The fasting blood glucose level of CO treated diabetic group was significantly lower than the diabetic group ( p < 0.05). Diabetic and CO treated diabetic groups showed a significant increase in the percentage of spleen CD3 + CD4 + T lymphocytes ( p < 0.05) when compared with the normal group. However, there was no significant difference in the percentage of spleen CD3 + CD8 + T lymphocytes among all experimental groups. The finding suggested that an aqueous extract of CO leaves has the ability to reduce blood glucose levels in diabetic rats. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate the antimicrobial potential of methanol, acetone and distilled water stem bark extracts from Canarium odontophyllum against Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 6633, Escherichia coli ATCC 25932, Pseudomonas aeruginosa ATCC 27853, Acinetobacter baumannii strain sensitive, Candida albicans ATCC 64677, Candida glabrata ATCC 90028, Aspergillus niger and Fusarium solani M2781. The extracts from C. odontophyllum stem bark from 3.125 mg/ml to 25 mg/ml were screened against the tested microorganisms using disc diffusion method. The Minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extracts against susceptible organisms were determined using microbroth dilution method and streak-plate technique, respectively. From the antibacterial screening assay, the growth of S. aureus, B. cereus and A. baumannii were inhibited by methanol extract whereas the acetone extract was capable of inhibiting all the tested microorganisms except E.coli, F. solani and A. niger. The lowest MIC value for methanol extract was against A. baumannii (0.195 mg/ml) whereas its MBC value was twice its MIC value (0.391 mg/ml), indicating that methanol extract was bacteriostatic against A. baumannii. While for acetone extract, S. aureus showed bactericidal effect with equal MIC and MBC values at 0.195 mg/ml. In conclusion, stem bark of C. odontophyllum has the potential to be the source of antibacterial agent and can be exploited as an alternative phytoantimicrobial. [ABSTRACT FROM AUTHOR]

Background: Canarium odontophyllum Miq. is a plant species widely known as 'dabai' and can be vastly found in Sarawak. The aim of this study was to assess the cytotoxic and genotoxic effect of extracts from stem bark of C odontophyllum against HCT 116 human colorectal cancer cell line. Method: The IC50 values of the aqueous, methanol, and acetone extracts against HCT 116 cells as well as the acetone extract against human colon fibroblast cell line CCD-18co were determined using the MTT assay. The concentration of the extracts ranged from 12.5 to 200 µg/ml at treatment time of 24,48 and 72 h. Annexin V-FITC/PI labelling assay was employed to determine mode of HCT 116 cell death induced by acetone extract at 48 h. The DNA damage induced by the extract in HCT 116 cells was detected using alkaline comet assay at 30 min of IC10 and IC25 treatment. Results: Acetone extract exhibited the highest cytotoxic effect against HCT 116 cells compared to methanol and aqueous extract at 24,48 and 72 h. Despite no cytotoxic effect by acetone extract against CCD-18co cells at 24 and 48 h, however at 72 h, CCD-18co cells proliferated. Apoptosis assessment using Annexin V-FITC/PI labelling assay revealed that the primary cell death was via apoptosis after 48 h treatment. Low doses of acetone extract from stem bark of C. odontophyllum showed significant DNA damage in HCT 116 cells with tail moment of 6.187 ±0.718 A.U and 7.877 ± 0.142 A.U, respectively. Conclusions: Acetone extract from stem bark of C. odontophyllum has high potential in the development of anticancer agent against HCT 116 cells with no cytotoxic effect against human colon fibroblast cells. [ABSTRACT FROM AUTHOR]

This study aimed to determine the protective effects of CO pulp and kernel oils supplementation to normocholesterolemic and hypercholesterolemic rabbits. Rabbits from the treatment groups were supplemented with CO pulp and kernel oils for four weeks. Bloods were drawn from all experimental groups at baseline and fourth week to determine protective effects of CO oils supplementation on plasma total antioxidant status (TAS) and catalase (CAT) activity. Liver function tests (ALT, AST, and GGT activities) were also determined for all the groups. The results showed that CO oil supplementation increased plasma TAS in both normal and hypercholesterolemic groups. Plasma CAT activities in the hypercholesterolemic groups supplemented with CO oils were significantly reduced but not for the normocholesterolemic groups. Significant reduction of plasma AST was observed for the hypercholesterolemic rabbits given CO pulp and kernel oils compared with the hypercholesterolemic control rabbits, but not for plasma ALT and GGT. In the normocholesterolemic rabbits, CO pulp oil had caused a significant elevation of plasma ALT, AST, and GGT levels as compared to the negative control rabbits. Therefore, CO pulp and kernel oils are somehow not hepatotoxic, and the oils are potent functional foods. [ABSTRACT FROM AUTHOR]

Highlights: [•] Layered co-sensitization was evaluated in order to achieve high DSSC performance. [•] Natural pigments from Ixora and Canarium odontophyllum were extracted. [•] Adsorption studies were carried out for each extracted dyes. [•] Individual dyes, the mixture and layered co-sensitized were tested in DSSCs. [•] The layered co-sensitization gave the highest conversion efficiency among all. [ABSTRACT FROM AUTHOR]

Dabai ( Canarium odontophyllum) is a potential 'functional fruit'. Future commercialization of dabai fat as healthy oil may result in the accumulation of defatted dabai as a by-product. This study was carried out to determine the total phenolics and antioxidant capacity of defatted dabai parts as a new source of functional food and nutraceutical ingredient. In this study, defatted dabai parts were extracted using different extraction media (methanol, ethanol, ethyl acetate, acetone, and water) and analyzed for total phenolics, total flavonoids, total anthocyanins, and antioxidant capacity (ABTS radical scavenging and ferric-reducing antioxidant power assays) using spectrophotometric and high-performance liquid chromatography methods. Major phenolics in defatted dabai peel extracted using methanol were catechin and epigallocatechin while in water extract, major phenolic acid was ellagic acid. Defatted dabai peel also had higher anthocyanidin content than its pulp. The peel of a defatted dabai fruit extracted using methanol contained a high total phenolics and Trolox equivalent antioxidant capacity (TEAC). Ethyl acetate extract of defatted dabai parts had the least phenolics compared in ethanol and acetone extracts. Higher total phenolics and TEAC values were observed in water extract of a defatted dabai peel than ethanol, acetone, and ethyl acetate extracts. Hence, methanol extract of a defatted dabai peel could probably be used as a natural antioxidant. [ABSTRACT FROM AUTHOR]

Dabai ( Canarium odontophyllum Miq.) fruit is a popular seasonal indigenous fruit of Sarawak, Malaysia that is always appreciated as an exotic fruit by the local community. The present study was designed to identify and quantify the phenolic compounds of dabai fruits from different divisions of Sarawak. Difference between the purple and red varieties of the fruits was also investigated. Analysis of phenolic compounds was performed by reversed phase high-performance liquid chromatography coupled with diode array detector. Three detection wavelengths (280, 254 and 329 nm) were used to monitor all phenolic compounds (except for anthocyanidins) since they display absorbance maxima at different wavelengths. Characteristic spectra of eluted peaks provide useful information in confirming the identities of peaks. Hydrolysis of phenolic compounds of dabai fruits was insufficient under the milder conditions (30 °C, 2 h) employed thus resulted in unidentified peaks. Two phenolic acids (ellagic and vanillic acids), five flavonoids (catechin, epicatechin, epicatechin gallate, epigallocatechin gallate, apigenin) and ethyl gallate were detected in the fruits. Furthermore, three anthocyanidins (cyanidin, pelargonidin and delphinidin) and four anthocyanins (malvidin-3,5-di- O-glucoside, cyanidin-3- O-glucoside, cyanidin-3- O-rutinoside and peonidin-3- O-glucoside) were detected. All these phenolic compounds were reported in dabai fruits for the first time. The fruit variety affected the anthocyanidins and anthocyanins profile ( p < 0.01) but had little or no effect on the phenolic acids and flavonoids profile of the fruits. Within the same variety, diversity in environmental conditions had an impact on the quantitative differences of phenolic compounds ( p < 0.05). [ABSTRACT FROM AUTHOR]

Abstract: The present study sought to determine the nutritional composition and antioxidant properties of Canarium odontophyllum Miq. (dabai) fruits from different districts in Malaysia, namely Kanowit, Sarikei, Kapit and Song in Sarawak. Two varieties of the fruits were investigated. Lipid (21.16±4.71 to 25.76±3.03g/100g FW) was the major macronutrient in dabai fruits, while the predominant minerals were calcium, sodium and potassium. The fruit protein was rich in aspartic and glutamic acids which accounted for 45–49% of total amino acids. Purple dabai fruits from Kapit were found to contain the highest total phenolic levels, flavonoids and anthocyanin contents (p <0.01) and to exhibit the most significant antioxidant activities (p <0.01), using trolox equivalent antioxidant capacity (TEAC) and ferric reducing ability (FRAP) assays. Antioxidant activities were highly correlated with total phenolic and flavonoid contents of dabai fruits. [Copyright &y& Elsevier]

Abstract: This study aimed to evaluate the antioxidant capacities of various parts of the dabai (Canarium odontophyllum Miq.) fruit. The antioxidant capacities of C. odontophyllum were evaluated using a β-carotene bleaching assay, ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays. Total phenolic content of the dabai fruit was estimated using the Folin–Ciocalteu method. The antioxidant capacities ranked in the following order: skin>flesh+skin>flesh>kernel. Our findings show that a high correlation exists between total phenolic content and total antioxidant activity. This indicates that phenolic compounds could be the major contributors to the antioxidant capacity of C. odontophyllum fruit. Therefore, dabai fruit, especially the skin, can be recommended as a major source of natural antioxidants. [ABSTRACT FROM AUTHOR]

Abstract: Canarium odontophyllum Miq. fruit (dabai) is an important food and oil resource for the native people in Sarawak, Malaysia. However, there is little information regarding their oil composition. In this paper, fatty acid compositions, vitamin E contents and physicochemical properties of dabai pulp and kernel oils were studied. These parameters were compared with commercial olive and palm oils. The content of total saturated fatty acid was found to be 44.4% in dabai pulp, kernel (60.8%), palm oil (47.9%) and olive (25.5%) oils. Palmitic, myristic, oleic and linoleic acids were detected in dabai pulp oil (36.1, 5.8, 41.5 and 11.8%) dabai kernel oil (46.4, 9.3, 35.1 and 2.8%), palm oil (33.8, 9.2, 39.7 and 10.9%) and olive oil (9.9, 12.9, 64.4 and 5.1%). Vitamin E was not detected in the dabai pulp oil, while palm oil had the highest vitamin E content, followed by dabai kernel oil and olive oil. In addition, the physicochemical characteristics of dabai oils show better quality than the studied commercial oils. The present study also showed that dabai pulp and kernel oils have good fatty acid composition and a high potential to be developed into healthy cooking oils. [ABSTRACT FROM AUTHOR]

Dabai (Canarium odontophyllum Miq.) is a fruit that is often eaten by first blanching in hot water to make the flesh creamier and softer, before it is served as a snack or side dish. In this study, Dabai fruit was blanched at different temperatures between 60 and 100 °C, with an increment of 10 °C, for up to 10 min, and the kinetics of quality changes (color and texture) were studied. Kinetic models that were assessed for changes of color and texture were zero-order, first-order, and fractional conversion model. The results showed that L parameter had no change throughout the blanching process, while parameters a*, b*, chroma (C), and total color difference (TCD) resulted as significantly increased as the temperature and duration of blanching increased. However, the change of firmness was not significant due to minor changes of firmness as the temperature and time increased. In terms of kinetic models, zero and fractional-conversion order well described the changes of a* parameter; while zero, first and fractional conversion well described parameters b*, C and TCD. Change of firmness did not fit with zero or first-order. All of the kinetic models obeyed the Arrhenius equation. Thus, the fitted kinetic models can be used to design the blanching process of Dabai fruit. [ABSTRACT FROM AUTHOR]

In this novel study, oil was extracted from the kernel of an exotic indigenous species known as Canarium odontophyllum via an ultrasound-assisted process. The extraction process was optimized using response surface methodology (RSM) based on the Box-Behnken experimental design (BBD). The optimal conditions for the investigated parameters were determined as ultrasound amplitude level: 38.30%, ratio of n -hexane to kernel powder: 50:1 in mL/g, extraction time: 45.79 min, resulting in an oil extraction yield of 63.48%. For verification purposes, experiments were conducted using the same optimized values of the investigated parameters which resulted in the average oil yield of 63.27% and this prove the reliability of the regression model. The extracted oil's fatty acid composition was obtained using a gas chromatograph (GC) equipped with flame-ionization detection (FID). The low acid value of the extracted oil is another interesting finding. This is important because it circumvents pretreatment processes such as degumming and esterification prior to the transesterification process. Biodiesel was produced from the oil via ultrasound-assisted transesterification, with a yield of 95.2%. Physiochemical properties of the C. odontophyllum biodiesel were determined, and it was found that all the tested properties comply with fuel specifications based on ASTM D6751 and EN 14214 standards. Significant savings of 52.3% and 80.9% in energy consumption and extraction time, respectively were achieved via ultrasound-assisted extraction compared with the conventional Soxhlet extraction. This study establishes the foundation and the need to further explore the usage of C. odontophyllum as a potential feedstock for biodiesel production. Image 1 [ABSTRACT FROM AUTHOR]