781 results on '"Camundongos"'
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2. Estudo da influência do glúten sobre parâmetros comportamentais e bioquímicos em animais submetidos a modelos de doenças neuropsiquiátricas.
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Maria de Souza, Marcia, Krutzsch, Franciele, Viana Silva, Késia Pires, André Cazarin, Camila, Ramos Wollf, Fellippe, and Wilson Valachinski, Álex
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MENTAL illness risk factors ,ANIMAL behavior ,BIOCHEMISTRY ,BIOMARKERS ,BIOLOGICAL models ,FLUOXETINE ,GLUTEN ,NEUROPSYCHOLOGY ,HYPERGLYCEMIA ,ANIMAL experimentation ,LOW density lipoproteins ,RISK assessment ,NEUROPSYCHOLOGICAL tests ,WEIGHT gain ,MENTAL depression ,MEMORY disorders ,ANXIETY ,HIGH density lipoproteins ,MENTAL illness ,MICE ,GLUTEN-free diet ,DIAZEPAM - Abstract
Copyright of Revista da Associação Brasileira de Nutrição is the property of Associacao Brasileira de Nutricao and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2023
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3. Efeito do treinamento resistido com choque no diâmetro, força e peso muscular de camundongos C57BL/6
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Giovana Evelin de Oliveira Costa, Aldecy Batista de Sá Júnior, Ataualba Ramalho de Meirelles Filho, Magda Mendes Vieira, Mariana Rocha Alves, Alex Sander Freitas, and Vinicius Dias Rodrigues
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treinamento de resistência ,força ,camundongos ,Sports medicine ,RC1200-1245 - Abstract
Objetivo: Verificar os efeitos do treinamento resistido com choque no diâmetro, força e peso muscular de camundongos C57BL/6. Materiais e Métodos: Foi realizado um estudo experimental, onde os animais foram divididos em grupo controle (n=5) e grupo experimental (n=5). O grupo experimental realizou 21 sessões de exercício resistido. Foram realizadas seis séries de oito repetições com 90 segundos de intervalo entre as séries. A mensuração da força muscular (FM) relativa e absoluta dos quatro membros foi realizada por meio de um medidor de força de tração muscular (Bonther®). O teste Mann-Whitney foi realizado para comparar a diferença pós-pré (delta) da variável dependente e o nível de significância foi estabelecido em p ≤ 0,05. Resultados: Os resultados encontrados mostraram que a diferença na FM absoluta média foi significativa (p=0,016), mas na FM absoluta máxima (p=0,076), na FM relativa média (p=0,175) e na FM relativa máxima (p=0,076) não ocorreu diferença significativa. Com relação ao tamanho do efeito, a FM absoluta média foi classificada como grande (1,13), a FM absoluta máxima foi classificada como grande (1,40), a FM relativa média foi classificada como moderada (0,92) e a FM relativa máxima foi classificada como grande (1,46). Conclusão: O treinamento resistido com estímulo de choque promoveu aumento de força muscular nos membros dos animais do grupo experimental, mostrando uma perspectiva para a continuidade das pesquisas com o objetivo de investigar o efeito desse treinamento em diversas situações relacionadas a saúde e/ou desempenho.
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- 2021
4. Avaliação dos Efeitos Cardíacos de Lectina Solúvel em Água (WSMoL) de Sementes de Moringa Oleifera
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Ainhoa Rodríguez de Yurre, José Dayvid Ferreira da Silva, Marília Kalinne da Silva Torres, Eduarda Lopes Martins, Isalira Peroba Ramos, Wênio Sandoval Filho Lima da Silva, Jéssica da Silva Sarpa, Caio César da Silva Guedes, Thiago Henrique Napoleão, Luana Cassandra Breitenbach Barroso Coelho, Patrícia Maria Guedes Paiva, and Emiliano Medei
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Moringa Oleifera ,Lectinas ,Glicosídeos ,Carboidratos ,Coração ,Segurança Hídrica ,Camundongos ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Resumo Fundsamento As sementes de Moringa oleifera , que são utilizadas para clarificação de água, contêm uma lectina chamada WSMoL que tem mostrado atividade antibacteriana e imunomoduladora in vitro . Devido ao seu valor nutritivo e potencial terapêutico, as folhas e as sementes dessa árvore são consumidas em algumas comunidades. Algumas lectinas de plantas não são tóxicas para mamíferos, mas tem sido relatado que outras são prejudiciais quando ingeridas ou administradas por outros meios. Objetivo Como um dos passos necessários para determinar a segurança de WSMoL, nós avaliamos os possíveis efeitos cardiotóxicos desta proteína purificada. Métodos Durante 21 dias consecutivos, a WSMoL foi administrada a camundongos por gavagem. Foram investigadas as funções eletrofisiológicas, mecânicas e metabólicas in vivo e ex vivo por meio de registros eletrocardiográficos, ressonância magnética nuclear e respirometria de alta resolução. Resultados O tratamento com WSMoL não induziu alterações nos níveis de glicose no sangue ou peso corporal em comparação com o grupo controle. Adicionalmente, as relações peso cardíaco/peso corporal e peso cardíaco/comprimento tibial estavam semelhantes em ambos os grupos. A ingestão de lectina também não modificou a tolerância à glicose ou resistência à insulina. Não foram observadas alterações nos parâmetros eletrocardiográficos ou na duração do potencial de ação cardíaco. Os corações dos camundongos dos grupos controle e WSMoL mostraram função ventricular esquerda preservada. Além disso, a WSMoL não induziu alterações na função mitocondrial (em todos os casos, p > 0,05). Conclusões A administração de WSMoL demonstrou ter um perfil de segurança cardíaca. Estes resultados contribuem à avaliação de segurança do uso de sementes de M. oleifera para tratar água, visto que essa lectina está presente na preparação empregada por algumas populações com esse fim. (Arq Bras Cardiol. 2020; [online].ahead print, PP.0-0)
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- 2020
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5. Toxicidade aguda em camundongos BALB/c expostos a efluentes de curtume
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Dalilla Cristina Socorro de Lemos, Bianca Costa e Silva, Joyce Moreira de Souza, Wellington Alves Mizael da Silva, Dieferson da Costa Estrela, Raissa Ferreira de Oliveira, Abraão Tiago Batista Guimarães, and Guilherme Malafaia
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Toxicologia ,Modelos experimentais ,Curtume ,Camundongos ,Education (General) ,L7-991 ,Science - Abstract
O processamento da pele bovina constitui uma das atividades industriais que geram resíduos altamente tóxicos, por conterem elevadas concentrações de metais pesados, como cromo, cádmio, níquel e chumbo. Sabe-se que o descarte inadequado desses efluentes no ambiente pode incorrer em consequências danosas aos organismos de magnitude complexa e pouco conhecida. Nesse contexto, objetivou-se com o presente estudo determinar doses potencialmente letais de efluentes de curtume, por meio da indução de toxicidade aguda em camundongos da linhagem BALB/c, acompanhada de uma avaliação de parâmetros físicos (massa corpórea e massa relativa de distintos órgãos), bioquímicos e comportamentais. 24 fêmeas de camundongos BALB/c foram distribuídas em grupos que receberam, pela via intraperitoneal, duas doses fracionadas de efluentes de curtume (wet blue), diluídos em água, que totalizaram as seguintes doses: 11,25%, 22,5% e 45%. O grupo controle recebeu apenas soro fisiológico. Os encontrados neste estudo não apontam para qualquer alteração física na massa corpórea dos animais, tampouco nas massas relativas do fígado, baço, timo e rins. Além disso, nenhuma mudança comportamental pelo screening hipocrático foi observada nos animais, com exceção de um animal do grupo 45% que foi a óbito no 3º dia de avaliação, o que corresponde para essa dose uma concentração de efluente de curtume que mata 16,66% dos animais, não sendo possível calcular a DL50. Em relação aos parâmetros bioquímicos, foi observada uma diminuição nas concentrações séricas da enzima fosfatase alcalina nos animais que receberam efluentes de curtume, em relação ao grupo controle. Logo, conclui-se que efluentes de curtume do tipo wet blue, administrados intraperitonealmente, em fêmeas de camundongos BALB/c não causam sinais evidentes de toxicidade aguda nos animais. A DL16,16 foi obtida apenas para a maior dose de efluente pré-estabelecida (45%).
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- 2018
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6. Ação moduladora da genotoxicidade da Solanum lycocarpum St. Hil. em micronúcleos induzidos pela ciclofosfamida
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Paula Moiana da Costa, Heleno Dias Ferreira, Pedro Henrique Ferri, Suzana da Costa Santos, Lídia Andreu Guillo, and Lee Chen Chen
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Antigenotoxicidade ,camundongos ,ciclofosfamida ,micronúcleo ,Solanum lycocarpum ,Biology (General) ,QH301-705.5 ,Ecology ,QH540-549.5 - Abstract
Ver resumo em arquivo em pdf.
- Published
- 2018
7. EFEITO DO TREINAMENTO RESISTIDO COM CHOQUE NO DIÂMETRO, FORÇA E PESO MUSCULAR DE CAMUNDONGOS C57BL/6.
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de Oliveira Costa, Giovana Evelin, Batista de Sá Júnior, Aldecy, de Meirelles Filho, Ataualba Ramalho, Mendes Vieira, Magda, Rocha Alves, Mariana, Sander Freitas, Alex, and Dias Rodrigues, Vinicius
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MUSCLE strength ,LABORATORY mice ,RESISTANCE training ,LABORATORY animals ,STRENGTH training ,ISOMETRIC exercise ,EXPERIMENTAL groups ,DEPENDENT variables - Abstract
Copyright of Revista Brasileira de Prescrição e Fisiologia do Exercício is the property of Instituto Brasileiro de Pesquisa e Ensino em Fisiologia do Exercicio and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2020
8. Sericin as treatment of obesity: morphophysiological effects in obese mice fed with high-fat diet.
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Kunz, Regina Inês, Novi Capelassi, Angélica, Paiva Alegre-Maller, Ana Cláudia, Bonfleur, Maria Lúcia, de Fátima Chasko Ribeiro, Lucinéia, Meire Costa, Rose, and Marçal Natali, Maria Raquel
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OBESITY treatment , *SERICIN , *HIGH-fat diet , *LABORATORY mice , *DRUG efficacy - Abstract
Objective: To investigate the effects of sericin extracted from silkworm Bombyx mori cocoon on morphophysiological parameters in mice with obesity induced by high-fat diet. Methods: Male C57Bl6 mice aged 9 weeks were allocated to one of two groups - Control and Obese, and fed a standard or high-fat diet for 10 weeks, respectively. Mice were then further subdivided into four groups with seven mice each, as follows: Control, Control-Sericin, Obese, and Obese-Sericin. The standard or high fat diet was given for 4 more weeks; sericin (1,000mg/kg body weight) was given orally to mice in the Control-Sericin and Obese-Sericin Groups during this period. Weight gain, food intake, fecal weight, fecal lipid content, gut motility and glucose tolerance were monitored. At the end of experimental period, plasma was collected for biochemical analysis. Samples of white adipose tissue, liver and jejunum were collected and processed for light microscopy analysis; liver fragments were used for lipid content determination. Results: Obese mice experienced significantly greater weight gain and fat accumulation and had higher total cholesterol and glucose levels compared to controls. Retroperitoneal and periepididymal adipocyte hypertrophy, development of hepatic steatosis, increased cholesterol and triglyceride levels and morphometric changes in the jejunal wall were observed. Conclusion: Physiological changes induced by obesity were not fully reverted by sericin; however, sericin treatment restored jejunal morphometry and increased lipid excretion in feces in obese mice, suggesting potential anti-obesity effects. [ABSTRACT FROM AUTHOR]
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- 2020
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9. Reduction of oxidative stress improves insulin signaling in cardiac tissue of obese mice.
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Scarpatto Rodrigues, Matheus, da Silva Pieri, Bruno Luiz, de Bem Silveira, Gustavo, Pereira Zaccaron, Rubya, Milanez Venturini, Ligia, Comin, Vitor Hugo, Damian Luiz, Karine, and Lock Silveira, Paulo Cesar
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OXIDATIVE stress , *INSULIN resistance , *CELLULAR signal transduction , *MYOCARDIUM , *ACETYLCYSTEINE , *LABORATORY mice - Abstract
Objective: To evaluate the effects of oxidative stress on insulin signaling in cardiac tissue of obese mice. Methods: Thirty Swiss mice were equally divided (n=10) into three groups: Control Group, Obese Group, and Obese Group Treated with N-acetylcysteine. After obesity and insulin resistance were established, the obese mice were treated with N-acetylcysteine at a dose of 50mg/kg daily for 15 days via oral gavage. Results: Higher blood glucose levels and nitrite and carbonyl contents, and lower protein levels of glutathione peroxidase and phosphorylated protein kinase B were observed in the obese group when compared with their respective control. On the other hand, treatment with N-acetylcysteine was effective in reducing blood glucose levels and nitrite and carbonyl contents, and significantly increased protein levels of glutathione peroxidase and phosphorylated protein kinase B compared to the Obese Group. Conclusion: Obesity and/or a high-lipid diet may result in oxidative stress and insulin resistance in the heart tissue of obese mice, and the use of N-acetylcysteine as a methodological and therapeutic strategy suggested there is a relation between them. [ABSTRACT FROM AUTHOR]
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- 2020
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10. Muscular resistance, hypertrophy and strength training equally reduce adiposity, inflammation and insulin resistance in mice with diet-induced obesity.
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Mansur Guedes, Janesca, da Silva Pieri, Bruno Luiz, Fernandes Luciano, Thaís, de Oliveira Marques, Schérolin, Antonacci Guglielmo, Luiz Guilherme, and de Souza, Claudio Teodoro
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HYPERTROPHY , *STRENGTH training , *OBESITY , *INFLAMMATION , *INSULIN resistance - Abstract
Objective: To evaluate the effect of three types of muscular resistance training on adiposity, inflammation levels and insulin activity in Swiss mice with fat-rich diet-induced obesity. Methods: Lean and obese male Swiss mice were selected and allocated to one of eight groups comprising eight mice each, as follows: standard diet + no training; standard diet + muscular resistance training; standard diet + hypertrophy training; standard diet + strength training; high-fat diet + no training; high-fat diet + muscular resistance training; high-fat diet + hypertrophy training; high-fat diet + strength training. The training protocol consisted of stair climbing for a 10-week period. Blood samples were collected for lactate analysis, glucose level measurement and insulin tolerance test. After euthanasia, adipose tissues were removed and weighed for adiposity index determination. Fragments of epididymal adipose tissue were then embedded for histological analysis or homogenized for tumor necrosis factor alpha level determination using the ELISA method. Results: Ausency of differences in total training volume and blood lactate levels overall emphasize the similarity between the different resistance training protocols. Body weight loss, reduced adipocyte area and lower adiposity index were observed in trained obese mice, regardless of training modality. Different training protocols also improved insulin sensitivity and reduced inflammation levels. Conclusion: Resistance training protocols were equally effective in reducing body fat, inflammation levels and insulin resistance in obese mice. [ABSTRACT FROM AUTHOR]
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- 2020
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11. Autophagy deficiency abolishes liver mitochondrial DNA segregation
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Katiane Tostes, Angélica C. dos Santos, Lindomar O. Alves, Luiz R. G. Bechara, Rachel Marascalchi, Carolina H. Macabelli, Mateus P. Grejo, William T. Festuccia, Roberta A. Gottlieb, Julio C. B. Ferreira, and Marcos R. Chiaratti
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Adult ,Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone ,Ribosomal Proteins ,Ubiquinone ,Iron ,Ubiquitin-Protein Ligases ,DNA, Mitochondrial ,Electron Transport Complex IV ,Mitochondrial Proteins ,Electron Transport Complex III ,Mice ,Adenosine Triphosphate ,Sequestosome-1 Protein ,Autophagy ,NADP Transhydrogenases ,Animals ,Humans ,PPAR alpha ,Molecular Biology ,Ubiquitins ,Apolipoproteins B ,Mitophagy ,Cell Biology ,Carbon Dioxide ,Cytochromes b ,Peptidylprolyl Isomerase ,NAD ,DNA-Binding Proteins ,Mice, Inbred C57BL ,Succinate Dehydrogenase ,Apolipoproteins ,Liver ,Proto-Oncogene Proteins c-bcl-2 ,CAMUNDONGOS ,Microtubule-Associated Proteins ,Protein Kinases ,Sulfur ,Transcription Factors ,Research Paper - Abstract
Mutations in the mitochondrial genome (mtDNA) are ubiquitous in humans and can lead to a broad spectrum of disorders. However, due to the presence of multiple mtDNA molecules in the cell, co-existence of mutant and wild-type mtDNAs (termed heteroplasmy) can mask disease phenotype unless a threshold of mutant molecules is reached. Importantly, the mutant mtDNA level can change across lifespan as mtDNA segregates in an allele- and cell-specific fashion, potentially leading to disease. Segregation of mtDNA is mainly evident in hepatic cells, resulting in an age-dependent increase of mtDNA variants, including non-synonymous potentially deleterious mutations. Here we modeled mtDNA segregation using a well-established heteroplasmic mouse line with mtDNA of NZB/BINJ and C57BL/6N origin on a C57BL/6N nuclear background. This mouse line showed a pronounced age-dependent NZB mtDNA accumulation in the liver, thus leading to enhanced respiration capacity per mtDNA molecule. Remarkably, liver-specific atg7 (autophagy related 7) knockout abolished NZB mtDNA accumulat ion, resulting in close-to-neutral mtDNA segregation through development into adulthood. prkn (parkin RBR E3 ubiquitin protein ligase) knockout also partially prevented NZB mtDNA accumulation in the liver, but to a lesser extent. Hence, we propose that age-related liver mtDNA segregation is a consequence of macroautophagic clearance of the less-fit mtDNA. Considering that NZB/BINJ and C57BL/6N mtDNAs have a level of divergence comparable to that between human Eurasian and African mtDNAs, these findings have potential implications for humans, including the safe use of mitochondrial replacement therapy.Abbreviations: Apob: apolipoprotein B; Atg1: autophagy-related 1; Atg7: autophagy related 7; Atp5a1: ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1; BL6: C57BL/6N mouse strain; BNIP3: BCL2/adenovirus E1B interacting protein 3; FCCP: carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; MAP1LC3A: microtubule-associated protein 1 light chain 3 alpha; MAP1LC3B: microtubule-associated protein 1 light chain 3 beta; mt-Atp8: mitochondrially encoded ATP synthase 8; MT-CO1: mitochondrially encoded cytochrome c oxidase I; MT-CO2: mitochondrially encoded cytochrome c oxidase II; mt-Co3: mitochondrially encoded cytochrome c oxidase III; mt-Cytb: mitochondrially encoded cytochrome b; mtDNA: mitochondrial DNA; MUL1: mitochondrial ubiquitin ligase activator of NFKB 1; nDNA: nuclear DNA; Ndufa9: NADH:ubiquinone oxireductase subunit A9; NDUFB8: NADH:ubiquinone oxireductase subunit B8; Nnt: nicotinamide nucleotide transhydrogenase; NZB: NZB/BINJ mouse strain; OXPHOS: oxidative phosphorylation; PINK1: PTEN induced putative kinase 1; Polg2: polymerase (DNA directed), gamma 2, accessory subunit; Ppara: peroxisome proliferator activated receptor alpha; Ppia: peptidylprolyl isomerase A; Prkn: parkin RBR E3 ubiquitin protein ligase; P10: post-natal day 10; P21: post-natal day 21; P100: post-natal day 100; qPCR: quantitative polymerase chain reaction; Rpl19: ribosomal protein L19; Rps18: ribosomal protein S18; SD: standard deviation; SEM: standard error of the mean; SDHB: succinate dehydrogenase complex, subunit B, iron sulfur (Ip); SQSTM1: sequestosome 1; Ssbp1: single-stranded DNA binding protein 1; TFAM: transcription factor A, mitochondrial; Tfb1m: transcription factor B1, mitochondrial; Tfb2m: transcription factor B2, mitochondrial; TOMM20: translocase of outer mitochondrial membrane 20; UQCRC2: ubiquinol cytochrome c reductase core protein 2; WT: wild-type.
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- 2023
12. Long-term treatment with 3,4-methylenedioxymethamphetamine caused retina damage in C57BL/6 mice.
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Xiangqin Liu and Hong Ye
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ENZYME-linked immunosorbent assay ,RETINA ,NERVE endings ,SALINE injections ,INTRAPERITONEAL injections - Abstract
Copyright of Arquivos Brasileiros de Oftalmologia is the property of Arquivos Brasileiros de Oftalmologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2019
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13. Avaliação da atividade biológica de preparações homeopáticas de antiadiponectina em camundongos submetidos a dieta hipercalórica.
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Martins, Maíra B., Martini, Lucienne C., and Bonamin, Leoni V.
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Adiponectin was originally identified as a protein expressed and produced by the abdominal fat adipocytes of several animal species which function is to maintain the homeostasis of the adipose tissue vis-à-vis the body energy demands. Homeopathic preparations of endogenous substances are able to modulate several cell functions. In the present study we investigated possible effects of a homeopathic preparation of anti-adiponectin antibodies on the control of the abdominal fat by means of an experimental model. Forty Swiss male adult mice were fed a commercial dietary supplement for 1 month and 7 days and treated with anti-adiponectin 6cH per oral route. Parameters assessed were weight gain and macro- and microscopic characteristics of the subcutaneous adipose tissue and liver. The experimental group exhibited slightly less weight gain and statistically significant reduction of the adipocyte diameter. We did not detect significant inflammatory or degenerative changes in the liver. The mechanisms involved in the detected changes need to be investigated in future studies. [ABSTRACT FROM AUTHOR]
- Published
- 2019
14. Coumestrol pre‐treatment improves spatial learning and memory deficits following transient cerebral ischemia recruiting hippocampal <scp>GluR2 AMPA</scp> receptors
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Cibele Canal Castro, Aline Souza Pagnussat, Carolina Demarchi Munhoz, and Carlos Alexandre Netto
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Memory Disorders ,Mice ,Neuroprotective Agents ,Coumestrol ,Ischemia ,Ischemic Attack, Transient ,Cognitive Neuroscience ,Spatial Learning ,Animals ,CAMUNDONGOS ,Receptors, AMPA ,Hippocampus ,Brain Ischemia - Abstract
Transient global ischemia is a leading cause of learning and memory dysfunction and induces a pattern of delayed neuronal death in the CA1 subfield of the hippocampus by down-regulating GluR2 mRNA AMPA receptors in this cerebral area. This study sought to investigate the neuroprotective effect of coumestrol against spatial memory impairment induced by global ischemia that leads to neural death by reducing the GluR2 receptors content in the hippocampal CA1 area. Our studies demonstrated that coumestrol administration prevented spatial memory deficits in mice. These findings suggest a cognitive enhancement role of coumestrol against cognitive impairment in ischemic events.
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- 2022
15. Efeito do treinamento de natação e suplementação de creatina sobre os níveis sanguíneos de lactato de camundongos
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Wendel Fernandes and Ricardo César Alves Ferreira
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treinamento físico ,camundongos ,lactato ,Sports medicine ,RC1200-1245 - Abstract
Introdução: A utilização de recursos ergogênicos associados aos protocolos de exercícios físicos é uma crescente, buscando o aumento do desempenho esportivo e benefícios estéticos. Objetivo: Demonstrar o potencial do protocolo de treinamento de natação e da suplementação de creatina em alterar os níveis sanguíneos de lactato de camundongos. Materiais e métodos: Treinamento de natação associado a suplementação de creatina e posterior teste de esforço em esteira para coleta de lactato. Discussão: A suplementação de creatina pode atuar aumentando a reversa muscular de creatina fosfato, favorecendo o metabolismo fosfagênico e diminuindo a formação de lactato. Resultados: Diminuição nos níveis sanguíneos de lactato tanto no grupo natação quanto no grupo natação associado a utilização de creatina. Conclusão: Diante dos resultados é possível concluir que o protocolo de exercício envolvendo a modalidade de natação apresenta potencial em diminuir o acúmulo sanguíneo de lactato de camundongos, bem como a suplementação de creatina associada ao protocolo de treinamento potencializa a redução no acúmulo dos níveis sanguíneos de lactato. ABSTRACT Effects of swimming training and creatine supplementation on blood levels of mice lactate Introduction: The use of ergogenic resources associated with the exercise protocols is a growing, seeking the increased sports performance and aesthetic benefits. Objective: To demonstrate the potential of swimming training protocol and creatine supplementation to alter blood levels of lactate mice. Methods: Swimming training associated with creatine supplementation and subsequent test on a treadmill to collect lactate. Discussion: Creatine supplementation can act increasing creatine phosphate in muscle, favoring this metabolism and decreasing the formation of lactate. Results: Decrease in blood lactate levels in the group that swimming and the swimming associated with the use of creatine group. Conclusion: With the results we conclude that the exercise protocol involving the mode of swimming evince the potential decrease blood lactate accumulation in mice and creatine supplementation associated with the training protocol enhances the reduction in the accumulation of blood levels of lactate.
- Published
- 2016
16. Anxiolytic-like effect of Citrus limon (L.) Burm f. essential oil inhalation on mice
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M.D.M. VIANA, R.M. CARDOSO, N.K.G.T. SILVA, M.A.P. FALCÃO, A.C.S. VIEIRA, M.S. ALEXANDRE-MOREIRA, and E.A. CAMPESATTO
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Ansiolítico ,Citrus limon ,Óleo essencial ,Inalação ,Camundongos ,Pharmacy and materia medica ,RS1-441 ,Botany ,QK1-989 - Abstract
ABSTRACT Experimental in vivo study aimed to characterize the anxiolytic-like effect of the Citrus limon fruit peel’s essential oil (CLEO) in animal models of anxiety, besides evaluating the viability J774.A1 cells in vitro through the MTT reduction method at the concentrations of 10 and 100 µg/mL. The anxiolytic behavior was evaluated in Swiss mice (n = 8) using the methodology of Elevated Plus-Maze (EPM) and Open-Field (OF). CLEO was tested by inhalation at the doses of 100, 200, and 400 µL, and as control, animals were subjected to inhalation of the vehicle (saline solution 0.9% + Tween80®) and intraperitoneal administration of diazepam (1.5 mg/kg). In the cell viability assay, it was observed that none of the concentrations showed cytotoxicity. OF test showed significant anxiolytic activity at all tested doses of OECL, compared to the control group, without changing the motor performance of the animals. Corroborating OF data, the EPM test confirmed anxiolytic activity in at least two doses of the tested oil (200 and 400 µL), justified by the number of entries and increase in the percentage of time in the open arms. The data analysis of this study evidenced that inhalation of OECL was able to induce an anxiolytic behavior in mice; however, further studies are required to ensure its safe use by the population.
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- 2016
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17. Intra-uterine experimental infection by Ureaplasma diversum induces TNF-α mediated womb inflammation in mice
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Jamile R. Silva, Lício F.A.A. Ferreira, Percíllia V.S. Oliveira, Ivanéia V. Nunes, Ítalo S. Pereira, Jorge Timenetsky, Lucas M. Marques, Tiana B. Figueiredo, and Robson A.A. Silva
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Micoplasma ,útero ,Ureaplasma ,camundongos ,Science - Abstract
Ureaplasma diversum is an opportunistic pathogen associated with uterine inflammation, impaired embryo implantation, infertility, abortions, premature birth of calves and neonatal pneumonia in cattle. It has been suggested that the intra-uterine infection by Ureaplasma diversum can cause vascular changes that hinder the success of pregnancy. Thus, the aim of this study was to evaluate the changes of intrauterine site of A/J mice in estrus or proestrus phase inoculated with Ureaplasma diversum. The infection was monitored at 24, 48 and 72 hours by the PCR methodology to detect the Ureaplasma in the inoculation site and the profile of circulating blood cells. Morphological changes, intensity of inflammation and the production of cytokines were compared. The infected mice showed local inflammation through the production of IFN-γ and TNF-α. Ureaplasma diversum infections in the reproductive tract of studied mice seemed to be associated with the production of pro-inflammatory cytokines in uterine parenchyma. The levels of TNF-α of infected mice were dependent on the bacterial load of inoculated Ureaplasma. Uterine experimental infections by Ureaplasma diversum have not been mentioned yet and herein we presented the first report of an intrauterine infection model in mice.
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- 2016
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18. Ablation of miRNA-22 protects against obesity-induced adipocyte senescence and ameliorates metabolic disorders in middle-aged mice
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Caroline A. Lino, Tábatha de Oliveira-Silva, Guilherme Lunardon, Camila Balbino-Silva, Vanessa M. Lima, Zhan-Peng Huang, Jose Donato Jr, Ana Paula C. Takano, Maria Luiza Barreto-Chaves, Da-Zhi Wang, and Gabriela P. Diniz
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Aging ,CAMUNDONGOS ,Developmental Biology - Published
- 2023
19. Moderate Continuous Aerobic Exercise Training Improves Cardiomyocyte Contractility in Β1 Adrenergic Receptor Knockout Mice
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Aurora Corrêa Rodrigues, Antônio José Natali, Daise Nunes Queiroz da Cunha, Alexandre Jayme Lopes Dantas Costa, Anselmo Gomes de Moura, Miguel Araújo Carneiro-Júnior, Leonardo Bonato Félix, Patrícia Chakur Brum, and Thales Nicolau Prímola-Gomes
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Insuficiência Cardíaca ,Exercício ,Contração Miocárdica ,Miócitos Cardíacos ,Antagonistas de Receptores Adrenérgicos beta 1 ,Camundongos ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Background: The lack of cardiac β1-adrenergic receptors (β1-AR) negatively affects the regulation of both cardiac inotropy and lusitropy, leading, in the long term, to heart failure (HF). Moderate-intensity aerobic exercise (MCAE) is recommended as an adjunctive therapy for patients with HF. Objective: We tested the effects of MCAE on the contractile properties of left ventricular (LV) myocytes from β1 adrenergic receptor knockout (β1ARKO) mice. Methods: Four- to five-month-old male wild type (WT) and β1ARKO mice were divided into groups: WT control (WTc) and trained (WTt); and β1ARKO control (β1ARKOc) and trained (β1ARKOt). Animals from trained groups were submitted to a MCAE regimen (60 min/day; 60% of maximal speed, 5 days/week) on a treadmill, for 8 weeks. P ≤ 0.05 was considered significant in all comparisons. Results: The β1ARKO and exercised mice exhibited a higher (p < 0.05) running capacity than WT and sedentary ones, respectively. The β1ARKO mice showed higher body (BW), heart (HW) and left ventricle (LVW) weights, as well as the HW/BW and LVW/BW than WT mice. However, the MCAE did not affect these parameters. Left ventricular myocytes from β1ARKO mice showed increased (p < 0.05) amplitude and velocities of contraction and relaxation than those from WT. In addition, MCAE increased (p < 0.05) amplitude and velocities of contraction and relaxation in β1ARKO mice. Conclusion: MCAE improves myocyte contractility in the left ventricle of β1ARKO mice. This is evidence to support the therapeutic value of this type of exercise training in the treatment of heart diseases involving β1-AR desensitization or reduction.
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- 2018
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20. The influence of enriched environment on spatial memory in Swiss mice of different ages
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Alessandra Fernandes Druzian, José Aparecido de Oliveira Melo, and Albert Schiaveto de Souza
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camundongos ,memória ,comportamento espacial ,hipocampo ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
The objective of this study was to evaluate the influence of enriched environment on spatial memory acquisition in mice of three different age groups. Weanling, young, and young adult female Swiss mice were housed in a standard control or enriched environment for 50 days, and their spatial memory was tested with the Morris Water Maze. We did not observe an experimental effect for spatial memory acquisition, and there was neither an effect of time of analysis nor an interaction between experimental group and time of analysis. Regarding effects of experimental group and training day in relation to latency in finding the hidden platform, we did find an effect in the experimental young adult mice group (p = 0.027), but there was no interaction between these factors in all three groups. Based on these findings environmental enrichment did not enhance spatial memory acquisition in female Swiss mice in the tested age groups.
- Published
- 2015
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21. Antinociceptive and anti-inflammatory effects of Lantana camara L. extract in mice
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T.S.C. SILVA, I.B. SUFFREDINI, E.L. RICCI, S.R.C. FERNANDES, V.JR GONÇALVES, P ROMOFF, J.H.G LAGO, and M.M. BERNARDI
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Lantana camara L. ,teste da placa quente ,estímulo térmico na cauda ,teste de contorções abdominais ,camundongos ,Pharmacy and materia medica ,RS1-441 ,Botany ,QK1-989 - Abstract
ABSTRACT:he Lantana camara L. belongs to the family Verbenaceae, which contains several active compounds in leaves and roots and which are reported to have medicinal and insecticidal properties. Studies of plants within the same family show the existence of anti-inflammatory activity in paw edema induced by carrageenan, serotonin and histamine and analgesic activity in the acetic acid writhing and tail-flick tests. The present study investigated whether the L. camara extract (ACE) also exerts these effects. The ACE toxicity was studied in male mice, and the percentage of mortality recorded 7 days after treatment was assessed. The ACE was evaluated as an antinociceptive agent in the hot plate, tail-flick and acetic acid writhing tests at a nontoxic dose of 1.0 g/Kg. The results showed that 1.5 g/Kg of ACE was not able to cause death, and doses of 3.0 and 4.0 g/Kg caused 50% and 60% death, respectively, in male mice. In all of the antinociceptive tests, 1 g/Kg of ACE markedly reduced responses to pain. Our findings suggest that ACE may have active anti-inflammatory and antinociceptive properties in much smaller doses than toxic.
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- 2015
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22. Western Diet-Fed ApoE Knockout Male Mice as an Experimental Model of Non-Alcoholic Steatohepatitis
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Felipe N. Camargo, Sandro L. Matos, Layanne C. C. Araujo, Carla R. O. Carvalho, Andressa G. Amaral, and João Paulo Camporez
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Microbiology (medical) ,CAMUNDONGOS ,General Medicine ,NASH ,NAFLD ,insulin resistance ,Western diet ,Molecular Biology ,Microbiology - Abstract
One of the consequences of the Western lifestyle and high-fat diet is non-alcoholic fatty liver disease (NAFLD) and its aggressive form, non-alcoholic steatohepatitis (NASH), which can progress to cirrhosis and hepatocellular carcinoma (HCC) and is rapidly becoming the leading cause of end-stage liver disease or liver transplantation. Currently, rodent NASH models lack significant aspects of the full NASH spectrum, representing a major problem for NASH research. Therefore, this work aimed to characterize a fast rodent model with all characteristic features of NASH. Eight-week-old male ApoE KO mice were fed with Western diet (WD), high fatty diet (HFD) or normal chow (Chow) for 7 weeks. Whole-body fat was increased by ~2 times in WD mice and HFD mice and was associated with increased glucose intolerance, hepatic triglycerides, and plasma ALT and plasma AST compared with Chow mice. WD mice also showed increased galectin-3 expression compared with Chow or HFD mice and increased plasma cholesterol compared with Chow mice. WD and HFD displayed increased hepatic fibrosis and increased F4/80 expression. WD mice also displayed increased levels of plasma MCP-1. Hepatic inflammatory markers were evaluated, and WD mice showed increased levels of TNF-α, MCP-1, IL-6 and IFN-γ. Taken together, these data demonstrated that the ApoE KO mouse fed with WD is a great model for NASH research, once it presents the fundamental parameters of the disease, including hepatic steatosis, fibrosis, inflammation, and metabolic syndrome.
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- 2022
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23. INFLUÊNCIA DO TRATAMENTO COM DOXICICLINA NA MORFOLOGIA DO ÍLEO DE CAMUNDONGOS INFECTADOS COM SCHISTOSOMA MANSONI.
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da SILVA, Thiago Donizeth, GONÇALVES, Reggiani Vilela, SOUZA, Raquel Lopes Martins, CAETANO, José Edson, CALDAS, Ivo Santana, MARQUES, Marcos José, and NOVAES, Rômulo Dias
- Abstract
Introdução: A esquistossomose mansoni é uma doença parasitária negligenciada causada pelo helminto Schistosoma mansoni. Essa doença está intimamente associada à pobreza e causa elevada morbidade êntero-hepática, associada à intensa reação inflamatória granulomatosa desencadeada pelos ovos retidos na microcirculação do fígado e intestino. Na enteropatia esquistossomótica, a inflamação granulomatosa está associada à erosão da parede intestinal e à translocação de ovos de S. mansoni até a luz intestinal, resultando na excreção desses ovos nas fezes do hospedeiro infectado. Objetivo: Investigar os efeitos do tratamento com doxiciclina sobre a morfologia do íleo de camundongos infectados por S. mansoni. Metodologia: Foram utilizados 40 camundongos suíços machos, divididos em 4 grupos: Grupo controle (GC) = não infectado, Grupo infectado (GI) = infectados por S. mansoni; Grupo infectado tratado com praziquantel (GIP) = infectados por S. mansoni tratados com 200 mg/kg de praziquantel, Grupo infectado tratado com doxiciclina (GID) = infectados por S. mansoni tratados com 50 mg/kg de hiclato de doxiciclina. Após 80 dias de infecção, os animais foram submetidos a um tratamento com praziquantel (dose única - 200 mg/kg) e hiclato de doxiciclina (dose diária de 50 mg/kg) por 60 dias. Os animais foram eutanasiados, e o íleo foi coletado para processamento histológico (corados com HE e Alcian Blue) e análise histomorfométrica. Resultado: Os grupos GC e GIP apresentaram uma microestrutura normal, com epitélio de revestimento intacto, suportado por lâmina própria, capilares linfáticos lacteais evidentes e células caliciformes bem definidas. Nos grupos GI e GID, as vilosidades intestinais mostraram-se ligeiramente delgadas. No Grupo GC, a submucosa apresenta-se delgada e com baixa celularidade. Nos grupos infectados, a submucosa apresenta-se espessa, com aumento do tecido conjuntivo e presença de granulomas. O Grupo GIP apresentou granulomas pequenos e com baixa celularidade na bainha. O grupo GID apresentou maiores granulomas e maior celularidade. Conclusão: Com base nos resultados obtidos neste estudo, podemos concluir que a infecção por Schistosoma mansoni causou significativas alterações na morfologia do íleo dos camundongos. Essas alterações incluíram o adelgaçamento das vilosidades intestinais, o espessamento da submucosa, o aumento do tecido conjuntivo e a formação de granulomas. Além disso, observamos que o tratamento com doxiciclina exacerbou essas alterações histomorfológicas, resultando em criptas intestinais mais rasas e uma área aumentada nos animais infectados. Esse efeito adverso da doxiciclina na resposta inflamatória e nas mudanças estruturais do íleo sugere a necessidade de cautela ao considerar essa substância como uma opção de tratamento para a esquistossomose mansoni. É importante destacar que o praziquantel, utilizado como tratamento de referência neste estudo, pareceu ser mais eficaz em limitar as alterações morfológicas induzidas pela infecção, com a preservação de uma microestrutura mais próxima do normal. [ABSTRACT FROM AUTHOR]
- Published
- 2023
24. Revisão sistemática sobre modelos experimentais de asma aguda e crônica induzidos com extrato de ácaro da poeira doméstica
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Moisés Santos Dutra, Cristian Roncada, Rodrigo Godinho de Souza, Aline Andrea da Cunha, and Paulo Márcio Pitrez
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Asma ,ácaro ,alérgeno ,camundongos ,Medicine - Abstract
Introdução: Asma é uma doença crônica das vias aéreas inferiores com elevada prevalência. Pesquisadores no mundo todo têm desenvolvido vários estudos experimentais em camundongos com o objetivo de entender melhor os mecanismos da doença e testar novas terapias. Ácaros estão presentes de forma abundante na poeira doméstica, sendo considerados os alérgenos mais comuns desencadeantes de asma alérgica. Este estudo objetiva apresentar e discutir desfechos inflamatórios no tecido pulmonar dos camundongos, verificar a diferença entre os modelos agudo e crônico de asma alérgica, tempo de exposição ao alérgeno, dose administrada e seu impacto nas pesquisas em modelos experimentais com asma. Métodos: A revisão da literatura foi realizada em quatro bancos de dados (PubMed, Scielo, Scopus e ScienceDirect). Os artigos selecionados foram avaliados primeiramente por dois pesquisadores de forma independente, de acordo com os critérios de inclusão. Resultados: Foram separados 126 artigos. Aplicados os critérios de inclusão e exclusão, somente 15 foram selecionados. São artigos que apresentaram diferentes protocolos de exposição ao HDM. A dose de HDM mais encontrada foi 100µg seguida por 25µg, e o tipo de modelo foi agudo. Conclusão: No modelo agudo, observa-se um elevado nível de inflamação das vias aéreas. Já o modelo crônico reproduz melhor as características da asma em humanos, hiper-responsividade brônquica e remodelamento das vias aéreas. Palavras-chave: Asma; ácaro; alérgeno; camundongos
- Published
- 2017
25. Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting
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Alan Carlos Alves Fernandes, Franciane Pereira de Oliveira, Gimena Fernandez, Luane da Guia Vieira, Cristiane Gugelmin Rosa, Taís do Nascimento, Suzelei de Castro França, Jose Donato, Kristen R. Vella, Jose Antunes-Rodrigues, André Souza Mecawi, Mario Perello, Lucila Leico Kagohara Elias, and Rodrigo Rorato
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CAMUNDONGOS ,General Biochemistry, Genetics and Molecular Biology - Abstract
Background The activation of the hypothalamic–pituitary–adrenal (HPA) axis is essential for metabolic adaptation in response to fasting. However, the neurocircuitry connecting changes in the peripheral energy stores to the activity of hypothalamic paraventricular corticotrophin-releasing factor (CRFPVN) neurons, the master controller of the HPA axis activity, is not completely understood. Our main goal was to determine if hypothalamic arcuate nucleus (ARC) POMC and AgRP neurons can communicate fasting-induced changes in peripheral energy stores, associated to a fall in plasma leptin levels, to CRFPVN neurons to modulate the HPA axis activity in mice. Results We observed increased plasma corticosterone levels associate with increased CRFPVN mRNA expression and increased CRFPVN neuronal activity in 36 h fasted mice. These responses were associated with a fall in plasma leptin levels and changes in the mRNA expression of Agrp and Pomc in the ARC. Fasting-induced decrease in plasma leptin partially modulated these responses through a change in the activity of ARC neurons. The chemogenetic activation of POMCARC by DREADDs did not affect fasting-induced activation of the HPA axis. DREADDs inhibition of AgRPARC neurons reduced the content of CRFPVN and increased its accumulation in the median eminence but had no effect on corticosterone secretion induced by fasting. Conclusion Our data indicate that AgRPARC neurons are part of the neurocircuitry involved in the coupling of PVNCRF activity to changes in peripheral energy stores induced by prolonged fasting.
- Published
- 2022
26. Modelos experimentales para la inducción de lesiones musculares en roedores: una revisión de la literatura
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Santos, Pammela Weryka da Silva, Santos, Thalyta Cibele Passos dos, Hazime, Fuad Ahmad, and Filgueiras, Marcelo de Carvalho
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Musculoesquelético ,Lesiones y heridas ,Musculoskeletal ,Modelos experimentales ,Modelo animal ,Lesions and wounds ,Animal model ,Modelos experimentais ,Lesões e feridas ,Ratones ,Camundongos ,Mice ,Experimental models - Abstract
Objective: the present study aims to evidence the most recurrent muscle injury techniques in the literature. Methodology: This is a bibliographical research of the integrative review type of literature. The articles were searched in 2021, from November 22 to 24, in the PubMed database, using the eligibility criteria. Results and Discussion: According to the search strategy used in this study, of all articles only 28 studies met the eligibility criteria. When analyzing the publication journals, it was observed that the most recurrent were PLoS One (7.14%), Int J Med Sci (7.14%), and J Trauma Acute Care with (7.14%). It was also evidenced that the number of publications on the subject has been growing over the years, when it compared the year 2016 (10.71%) with later years, except in 2019 with the same percentage of 10.71% and 2021 with zero publication. The breeds most used in the experiments were Sprague-Dawley (32.14%) and Wistar with 25%. There was a predominance of models due to contusion (35.71%), followed by excessive use injury (10.71%), and traumatic injury (10.71%), for induction of muscle injury in rodents. Conclusion: According to the results of this review, the most recurrent muscle injury induction models were injury by contusion, followed by excessive use injury, and traumatic injury. However, all the techniques addressed in the present study were able to reproduce with excellence the mechanism of muscle injury. Objetivo: el presente estudio tiene como objetivo destacar las técnicas de lesión muscular más recurrentes en la literatura. Metodología: Se trata de una investigación bibliográfica del tipo revisión integradora de literatura. Los artículos fueron buscados en el año 2021, del 22 al 24 de noviembre, en la base de datos PubMed, utilizando los criterios de elegibilidad. Resultados y Discusión: De acuerdo con la estrategia de búsqueda utilizada en este estudio, de todos los artículos, solo 28 estudios cumplieron con los criterios de elegibilidad. Al analizar las revistas de publicación, se observa que las más recurrentes fueron PLoS One (7,14%), Int J Med Sci (7,14%) y J Trauma Acute Care with (7,14%). También se evidenció que el número de publicaciones sobre el tema ha ido creciendo a lo largo de los años, al comparar el año 2016 (10,71%) con años posteriores, excepto en el 2019 con el mismo porcentaje de 10,71% y el 2021 con cero publicaciones. Las razas más utilizadas en los experimentos fueron Sprague-Dawley (32,14%) y Wistar con un 25%. Predominaron los modelos de contusión (35,71%), seguido de lesión por sobreuso (10,71%) y lesión traumática (10,71%), por inducir lesión muscular en roedores. Conclusión: Según los resultados de esta revisión, los modelos de inducción de lesiones musculares más recurrentes fueron las lesiones por contusión, seguidas de las lesiones por uso excesivo y las lesiones traumáticas. Sin embargo, todas las técnicas discutidas en el presente estudio pudieron reproducir con excelencia el mecanismo de lesión muscular. Objetivo: O presente estudo tem como objetivo evidenciar as técnicas de lesão muscular mais recorrentes na literatura. Metodologia: Trata-se de uma pesquisa bibliográfica do tipo revisão integrativa da literatura. Os artigos foram pesquisados no ano de 2021, no período de 22 a 24 de novembro, na base de dados PubMed, usando os critérios de elegibilidade. Resultados e Discussão: De acordo com a estratégia de busca utilizada neste estudo, da totalidade dos artigos apenas 28 estudos atenderam os critérios de elegibilidade. Ao analisar as revistas de publicação, observa-se que as mais recorrentes foram PLoS One (7.14%), Int J Med Sci (7.14%), e J Trauma Acute Care com (7.14%). Evidenciou-se também, que o número de publicações sobre a temática vem crescendo ao longo dos anos, quando compara o ano de 2016 (10.71%) com anos posteriores, exceto em 2019 com o mesmo percentual de 10.71% e 2021 com zero publicação. As raças mais utilizadas nos experimentos foram Sprague-Dawley (32.14%) e Wistar com 25%. Houve uma predominância dos modelos por contusão (35.71%), seguido da lesão por uso excessivo (10.71%), e por lesão traumática (10.71%), para indução de lesão muscular em roedores. Conclusão: Conforme os resultados desta revisão, os modelos de indução de lesão muscular mais recorrente foi a lesão por contusão, seguido da lesão por uso excessivo, e por lesão traumática. Porém, todas as técnicas abordadas no presente estudo conseguiram reproduzir com excelência o mecanismo de injúria muscular.
- Published
- 2022
27. Moderate Exercise Training Combined With a High-Fat and Sucrose Diet Protects Pancreatic Islet Function in Male C57BL/6J Mice
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Katherine, Veras, Camila Ferraz, Lucena, Julia, Goedcke, Fabiana S, Evangelista, Angelo, Carpinelli, and Carla Roberta de Oliveira, Carvalho
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Male ,Sucrose ,Endocrinology, Diabetes and Metabolism ,Caseins ,Starch ,Diet, High-Fat ,Mice, Inbred C57BL ,Islets of Langerhans ,Mice ,Glucose ,Animals ,Insulin ,Obesity ,CAMUNDONGOS ,Insulin Resistance - Abstract
Obesity is mainly caused by excess energy intake and physical inactivity, and the number of overweight/obese individuals has been steadily increasing for decades. Previous studies showed that rodents fed westernized diets exhibit endocrine pancreas deterioration and a range of metabolic disorders. This study evaluated the effects of moderated aerobic treadmill exercise training on pancreatic islet cell viability and function in mice consuming a high-fat and sucrose diet. In the present study, 60-day-old male C57BL/6J mice were divided into four groups: control (C), fed a standard diet AIN-93M (3.83 kcal/g; 70% carbohydrate (cornstarch and dextrinized starch were chosen as the major source of carbohydrate for the AIN-93 diet. In addition, a small amount of sucrose), 20% protein (casein), and 10% fat (soybean) with no training (i.e., sedentary); C + training (CTR, fed the standard diet with eight weeks of exercise; high-fat diet + sucrose (HFDS), fed a high fat and sucrose diet (5.2 kcal/g; 20% carbohydrate (cornstarch and dextrinized starch were chosen as the major source of carbohydrate), 20% protein (casein), 60% fat (Lard was chosen as the major source of fat and a small amount of soybean) + 20% sucrose diluted in drinking water with no training; and HFDS + training (HFDSTR). After eight weeks, the HFDS mice displayed increased body weight (P
- Published
- 2022
28. Oxidative damage induced by cigarette smoke exposure in mice: impact on lung tissue and diaphragm muscle
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Samanta Portão de Carlos, Alexandre Simões Dias, Luiz Alberto Forgiarini Júnior, Patrícia Damiani Patricio, Thaise Graciano, Renata Tiscoski Nesi, Samuel Valença, Adriana Meira Guntzel Chiappa, Gerson Cipriano Jr, Claudio Teodoro de Souza, and Gaspar Rogério da Silva Chiappa
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Estresse oxidativo ,Camundongos ,Sistema respiratório ,Poluição por fumaça de tabaco ,Inflamação ,Diseases of the respiratory system ,RC705-779 - Abstract
OBJECTIVE: To evaluate oxidative damage (lipid oxidation, protein oxidation, thiobarbituric acid-reactive substances [TBARS], and carbonylation) and inflammation (expression of phosphorylated AMP-activated protein kinase and mammalian target of rapamycin [p-AMPK and p-mTOR, respectively]) in the lung parenchyma and diaphragm muscles of male C57BL-6 mice exposed to cigarette smoke (CS) for 7, 15, 30, 45, or 60 days. METHODS: Thirty-six male C57BL-6 mice were divided into six groups (n = 6/group): a control group; and five groups exposed to CS for 7, 15, 30, 45, and 60 days, respectively. RESULTS: Compared with control mice, CS-exposed mice presented lower body weights at 30 days. In CS-exposed mice (compared with control mice), the greatest differences (increases) in TBARS levels were observed on day 7 in diaphragm-muscle, compared with day 45 in lung tissue; the greatest differences (increases) in carbonyl levels were observed on day 7 in both tissue types; and sulfhydryl levels were lower, in both tissue types, at all time points. In lung tissue and diaphragm muscle, p-AMPK expression exhibited behavior similar to that of TBARS. Expression of p-mTOR was higher than the control value on days 7 and 15 in lung tissue, as it was on day 45 in diaphragm muscle. CONCLUSION: Our data demonstrate that CS exposure produces oxidative damage, not only in lung tissue but also (primarily) in muscle tissue, having an additional effect on respiratory muscle, as is frequently observed in smokers with COPD.
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- 2014
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29. Expressao genica associada ao estresse oxidativo no coracao de camundongo apos isquemia intestinal
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Frederico Somaio Neto, Adauto Tsutomu Ikejiri, Paulo Roberto Bertoletto, Jose Carlos Bertoletto Chaves, Roberto Teruya, Djalma Jose Fagundes, and Murched Omar Taha
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Expressao Genica ,Camundongos ,Extresse Oxidativo ,Intestinos / patologia ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
FUNDAMENTO: O fenômeno da isquemia e reperfusão intestinal é um evento frequente na clínica e está associado a repercussões deletérias em órgãos a distância, em especial ao coração. OBJETIVO: Investigar a expressão gênica do estresse oxidativo e defesa antioxidante no coração de camundongos isogênicos, submetidos a isquemia e reperfusão intestinal (IR). MÉTODOS: Doze camundongos (C57BL/6) foram distribuídos em dois grupos: Grupo IR (GIR) com 60 min de oclusão da artéria mesentérica superior, seguidos de 60 min de reperfusão. Grupo Controle (GC) submetidos a anestesia e a laparotomia sem o procedimento de IR observados por 120 min. As amostras de intestino e coração foram processadas pelo método (RT-qPCR / Reverse transcriptase - quantitative Polymerase Chain Reaction) para determinar a expressão gênica de 84 genes relacionados ao estresse oxidativo ("t" de Student, p < 0,05). RESULTADOS: Observou-se no tecido intestinal (GIR) uma expressão significantemente aumentada em 65 (74,71%) genes em relação ao tecido normal (GC), e 37 (44,04%) genes estiveram hiperexpressos (maior que três vezes o limiar permitido pelo algoritmo). No tocante aos efeitos da I/R intestinal a distância no tecido cardíaco verificou-se a expressão significantemente aumentada de 28 genes (33,33%), mas somente oito genes (9,52%) se hiperexpressaram três vezes acima do limiar. Quatro (7,14%) desses oito genes se expressaram simultaneamente nos tecidos intestinal e cardíaco. No GIR notaram-se cardiomiócitos com núcleos de menor tamanho, picnóticos, ricos em heterocromatina e raros nucléolos, indicando sofrimento cardíaco. CONCLUSÃO: A I/R intestinal promoveu a hiperexpressão estatisticamente significante de oito genes associados ao estresse oxidativo a distância no tecido miocárdico.
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- 2014
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30. Um possível mecanismo de ação para o efeito anticonvulsivante do p-cimeno
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Talita Mendes de Oliveira, Rusbene Bruno Fonseca de Carvalho, Rivelilson Mendes de Freitas, and Sidney Gonçalo de Lima
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Anticonvulsivante ,Camundongos ,p-Cimeno ,Monoterpenos ,Pharmaceutical industry ,HD9665-9675 ,Pharmacy and materia medica ,RS1-441 - Abstract
O presente estudo teve como objetivo investigar o efeito anticonvulsivante do p-cimeno (CIM), bem como verificar a concentração das monoaminas (dopamina (DA), noradrenalina (NA), serotonina (5-HT), e seus metabólitos (ácido dihidroxifenilacético (DOPAC), ácido homovanílico (HVA) e ácido 5-hidroxi-indol-acético (5- HIAA)) no hipocampo de camundongos tratados com cloridrato de pilocarpina 400 mg/kg (P400; i.p.) e nos grupos tratados com a associação de CIM (50, 100 ou 150 mg/kg) e P400. Nesse estudo foi avaliada a latência para a primeira crise epiléptica e a taxa de mortalidade. Os resultados revelaram que o CIM produziu um aumento na latência para primeira crise epilética, bem como promoveu uma proteção significativa contra a mortalidade induzida pelo processo convulsivo. O monoterpeno nas doses testadas também foi capaz de produzir um aumento da latência para instalação do estado de mal epilético induzido por pilocarpina. Além disso, durante os estudos para identificar o mecanismo de ação nenhum dos efeitos do p-cimeno nesse modelo foram bloqueados pelo pré-tratamento com atropina. Complementando os estudos para identificar o provável mecanismo de ação do p-cimeno, foi verificado que os efeitos desse monorterpeno foram revertidos pelo flumazenil, um antagonista do sistema GABAérgico, sugerindo que esse monoterpeno pode atuar por meio desse sistema. Também, em nossos resultados também foi visto uma diminuição dos níveis de DA e um aumento do conteúdo de seus metabólitos (DOPAC e HVA) durante as crises epilépticas. Por outro lado foi detectado um aumento na concentração de NA e 5-HT, e uma diminuição no metabólito da 5-HT (5- HIAA) durante as crises epilépticas. Dessa forma, o CIM na presença do estímulo convulsivo, reverte os efeitos produzidos nos níveis das monoaminas e seus metabólitos observados durante as crises epilépticas, sugerindo que este monoterpeno produz efeito anticonvulsivante por meio da modulação direta do sistema GABAérgico e indiretamente por meio de modificações contrárias no conteúdo das monoaminas as observadas durante processo convulsivo (DA, NA e 5-HT) na região hipocampal. No entanto, mais estudos são necessários para esclarecer completamente o mecanismo de ação anticonvulsivante do p-cimeno.
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- 2014
31. Estudo das propriedades físico-químicas e avaliação da toxicidade aguda do extrato etanólico das folhas de Mikania glomerata Sprengel
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L.C.L.R. Santana, M.R.M. Brito, G.F. Sousa, and R.M. Freitas
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Mikania glomerata ,Asteraceae ,Toxicidade Aguda ,Camundongos ,Pharmacy and materia medica ,RS1-441 ,Botany ,QK1-989 - Abstract
A Mikania glomerata é uma planta pertencente à família Asteraceae que é bastante utilizada na medicina popular devido às suas ações broncodilatadora, antiasmática, expectorante e antitussígena. O objetivo do presente estudo foi determinar as propriedades físico-químicas do pó obtido a partir das folhas de M. glomerata, bem como, avaliar a toxicidade em camundongos após tratamento agudo com doses repetidas do extrato etanólico padronizado preparado a partir das folhas dessa espécie. Durante o estudo das propriedades físico-químicas do pó obtido a partir das folhas de M. glomerata foram feitas as determinações da densidade bruta e de compactação, do teor de cinzas totais, do teor de umidade, e da granulometria. De acordo com os resultados obtidos podemos sugerir que o pó pode ser usado na formulação de uma forma farmacêutica sólida. Na segunda parte do estudo foi determinada a Dose Letal 50% (DL50), bem como, realizada a análise morfológica macroscópica e avaliados a toxicidade aguda com doses repetidas e os parâmetros bioquímicos e hematológicos de camundongos. De acordo com os dados obtidos na segunda parte deste estudo podemos sugerir que o extrato etanólico pode ser usado de forma segura em humanos, uma vez que apresentou valor de DL50 de aproximadamente 3000 mg Kg-1), bem como, não produziu nenhuma alteração morfológica nos principais órgãos, e nem provocou alterações nos parâmetros bioquímicos e hematológicos de camundongos.
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- 2014
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32. Effects of the Isolated and Combined Ablation of Growth Hormone and IGF-1 Receptors in Somatostatin Neurons
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Fernanda M Chaves, Frederick Wasinski, Mariana R Tavares, Naira S Mansano, Renata Frazao, Daniela O Gusmao, Paula G F Quaresma, João A B Pedroso, Carol F Elias, Edward O List, John J Kopchick, Raphael E Szawka, and Jose Donato
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Male ,Neurons ,Human Growth Hormone ,Receptors, Somatotropin ,Receptor, IGF Type 1 ,Mice ,Endocrinology ,Growth Hormone ,Animals ,Female ,CAMUNDONGOS ,Insulin-Like Growth Factor I ,Somatostatin ,hormones, hormone substitutes, and hormone antagonists ,Research Article - Abstract
Hypophysiotropic somatostatin (SST) neurons in the periventricular hypothalamic area express growth hormone (GH) receptor (GHR) and are frequently considered as the key neuronal population that mediates the negative feedback loop controlling the hypothalamic–GH axis. Additionally, insulin-like growth factor-1 (IGF-1) may also act at the hypothalamic level to control pituitary GH secretion via long-loop negative feedback. However, to the best of our knowledge, no study so far has tested whether GHR or IGF-1 receptor (IGF1R) signaling specifically in SST neurons is required for the homeostatic control of GH secretion. Here we show that GHR ablation in SST neurons did not impact the negative feedback mechanisms that control pulsatile GH secretion or body growth in male and female mice. The sex difference in hepatic gene expression profile was only mildly affected by GHR ablation in SST neurons. Similarly, IGF1R ablation in SST neurons did not affect pulsatile GH secretion, body growth, or hepatic gene expression. In contrast, simultaneous ablation of both GHR and IGF1R in SST-expressing cells increased mean GH levels and pulse amplitude in male and female mice, and partially disrupted the sex differences in hepatic gene expression. Despite the increased GH secretion in double knockout mice, no alterations in body growth and serum or liver IGF-1 levels were observed. In summary, GHR and IGF1R signaling in SST neurons play a redundant role in the control of GH secretion. Furthermore, our results reveal the importance of GH/IGF-1 negative feedback mechanisms on SST neurons for the establishment of sex differences in hepatic gene expression profile.
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- 2022
33. Avalia??o in vitro e in vivo dos efeitos de ?cidos graxos de cadeia curta no c?ncer de mama
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Murad?s, Tha?s Cristina and Campos, Maria Martha
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Mice ,Camundongos ,MEDICINA [CIENCIAS DA SAUDE] ,C?ncer de Mama ,Cell Culture ,Breast Cancer ,?cidos Graxos de Cadeia Curta ,Modelo de C?ncer de Mama Murino 4T1 ,Met?stases ,Mouse 4T1 Breast Tumor Model ,Cultivo Celular ,Short-Chain Fatty Acids ,Metastasis - Abstract
O c?ncer de mama apresenta a maior incid?ncia mundial entre todos os tipos de tumores. Os tratamentos s?o direcionados conforme o subtipo molecular: luminais A e B, HER2+ e triplo negativo. Apesar das taxas de sucesso para os tipos hormonais e HER2+, os pacientes que apresentam recidivas para os tumores mais agressivos ou triplos negativos permanecem com baixa sobrevida geral e o progn?stico ? desfavor?vel devido, por exemplo, ? aus?ncia de um alvo terap?utico ou elevada agressividade. Ademais, altera??es na composi??o da microbiota e, consequentemente, dos ?cidos graxos de cadeia curta (SCFA) ? atrav?s das condi??es cl?nicas basais ou por efeitos colaterais dos tratamentos ? pode afetar o risco e o progn?stico para o c?ncer de mama. Neste sentido, este estudo avaliou os efeitos dos SCFA em diferentes modelos de c?ncer de mama com o prop?sito de identificar uma nova abordagem para o tratamento desses tumores. Os ensaios in vitro utilizando as linhagens celulares de c?ncer de mama de diferentes subtipos moleculares, humanas, MCF-7, SK-BR-3 e MDA-MB-231; ou murina, 4T1 exibiram efeitos contrastantes ap?s os tratamentos com os SCFA. Na an?lise da viabilidade celular, o acetato apresentou efeitos positivos em concentra??es acima de 100 mM. Enquanto o propionato apresentou efeito dual dependente da concentra??o. De maneira tempo e concentra??o-dependente, o butirato, propionato e valerato reduziram significativamente a viabilidade celular das linhagens humanas e, o valerato reduziu a viabilidade celular da linhagem murina sob o IC50 de 9,6 mM em 48 h. Por outro lado, os agonistas seletivos de FFA2 e FFA3, 4-CMTB e AR420626, respectivamente, reduziram modestamente a viabilidade celular apenas das linhagens mais agressivas MDA-MB-231 e 4T1. Da mesma forma que na avalia??o dos antagonistas de FFA2 e FFA3, CATPB e ?-hidroxibutirato, respectivamente, somente o CATPB produziu efeitos inibit?rios na linhagem 4T1. Esses dados sugerem que os SCFA apresentam efeitos antitumorais no c?ncer de mama provavelmente por mecanismos independentes ? liga??o aos receptores FFA2 e FFA3. A partir desse screening, os SCFA com melhores efeitos inibit?rios, butirato, propionato e valerato, foram selecionados para investigar seus efeitos na capacidade de malignidade nas c?lulas MDA-MB-231 e 4T1. Altera??es significativas na morfologia celular foram observadas, assim como na redu??o da forma??o de novas col?nias. Na avalia??o da ades?o celular, o valerato reduziu este par?metro na linhagem MDA-MB231, no entanto, aumentou em rela??o ? linhagem 4T1. Em rela??o ? avalia??o da migra??o celular, somente o tratamento com butirato (10 mM) preveniu a migra??o da linhagem 4T1 em 24 h. Apesar de discretos, estes resultados corroboram os efeitos antitumorais observados na redu??o da forma??o de col?nias e altera??es da morfologia celular ap?s a exposi??o com o butirato na linhagem 4T1, principalmente. Para investigar os efeitos desses SCFA na progress?o tumoral foi utilizado o modelo ortot?pico de c?ncer de mama metast?tico em camundongos f?mea Balb/CJ induzido pela inocula??o das c?lulas 4T1. Os resultados preliminares da administra??o por via oral dos SCFA tanto no protocolo terap?utico quanto no preventivo n?o demonstraram um efeito espelhado de acordo com a avalia??o in vitro, exceto pelo n?mero de met?stases pulmonares que foram significativamente reduzidas pelo butirato (600 mg/kg) no esquema terap?utico. Essas evid?ncias sugerem que a participa??o do microambiente tumoral est? envolvida na a??o dos SCFA, assim como seus efeitos celulares podem ser modulados por mecanismos alternativos aos receptores FFA2 e FFA3, como pela inibi??o de HDAC. Portanto, para estabelecer um tratamento promissor, a defini??o desses mecanismos pode contribuir na determina??o dos SCFA como um alvo terap?utico no manejo do c?ncer de mama. Breast cancer has the highest worldwide incidence of all types of tumors. Treatments are defined according to molecular subtypes: luminal A and B, HER2+, and triple-negative. Despite the success rates for hormonal and HER2+ types, patients who relapse for the most aggressive or triple-negative tumors remain with low overall survival and the prognosis is poor due, for example, to the absence of a therapeutic target or high aggressiveness. Furthermore, alterations in the microbiota composition, consequently, in short-chain fatty acids (SCFA) ? through baseline clinical conditions or side effects of treatments ? might affect the risk and prognosis for breast cancer. In this sense, this study evaluated the effects of SCFA in different models of breast cancer to identify a new approach for the treatment of these tumors. In vitro assays using breast cancer cell lines of different molecular subtypes, human (MCF-7, SK-BR-3, and MDA-MB-231) or murine (4T1) exhibited contrasting effects after treatments with SCFA. In the analysis of cell viability, acetate showed positive effects at concentrations above 100 mM. While propionate demonstrated a dual concentration-dependent effect. In a time and concentration-dependent manner, butyrate, propionate, and valerate significantly reduced the cell viability of human cells, and valerate reduced the viability of the murine cells under an IC50 of 9.6 mM in 48 h. On the other hand, selective FFA2 and FFA3 agonists 4-CMTB and AR420626, respectively, modestly reduced cell viability only of the more aggressive MDA-MB-231 and 4T1 cell lines. As in the evaluation of the FFA2 and FFA3 antagonists CATPB and ?hydroxybutyrate, respectively, only CATPB produced inhibitory effects in the 4T1 cells. These data suggest that SCFA have antitumor effects in breast cancer, probably through FFA2 and FFA3 receptor independent mechanisms. From this screening, SCFAs with best inhibitory effects, butyrate, propionate, and valerate, were selected to investigate their effects on the malignancy capacity of MDAMB-231 and 4T1 cells. Several changes in cell morphology were observed, as well as a reduction in the new colonies formation. In the evaluation of cell adhesion, valerate reduced this parameter in the MDA-MB-231 cells, however, it increased about the 4T1 cells. Regarding the evaluation of cell migration, only the treatment with butyrate (10 mM) prevented the migration of the 4T1 cells within 24 h. Although discreet, these results corroborate the antitumor effects observed in the reduction of colony formation and changes in cell morphology after exposure with butyrate in the 4T1 cells, mainly. To investigate the effects of these SCFAs on tumor progression, was used the orthotopic model of metastatic breast cancer in female Balb/CJ mice induced by inoculation of 4T1 cells. The preliminary results of oral administration of SCFA in both the therapeutic and preventive protocols did not demonstrate a mirror effect according to the in vitro evaluation, except for the number of lung metastases that were significantly reduced by butyrate (600 mg/kg) in the therapeutic scheme. These evidence suggests that the participation of the tumor microenvironment is involved in the action of SCFA, as well as their cellular effects can be modulated by alternative mechanisms to the FFA2 and FFA3 receptors, such as the HDACs inhibition. Therefore, to establish a promising treatment, the definition of these mechanisms might contribute to the determination of SCFA as a therapeutic target in the management of breast cancer. Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES
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- 2022
34. Characterization of the metabolic differences between male and female C57BL/6 mice
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Gabriel O. de Souza, Frederick Wasinski, and Jose Donato
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Leptin ,Male ,Hypothalamus ,General Medicine ,General Biochemistry, Genetics and Molecular Biology ,Ghrelin ,Mice, Inbred C57BL ,Mice ,Growth Hormone ,Animals ,Female ,CAMUNDONGOS ,Obesity ,General Pharmacology, Toxicology and Pharmaceutics - Abstract
The present study aims to compare the responses between male and female C57BL/6 mice to multiple metabolic challenges to understand the importance of sex in the control of energy homeostasis.Male and female C57BL/6 mice were subjected to nutritional and hormonal challenges, such as food restriction and refeeding, diet-induced obesity, feeding response to ghrelin and leptin, ghrelin-induced growth hormone secretion, and central responsiveness to ghrelin and leptin. The hypothalamic expression of transcripts that control energy homeostasis was also evaluated.Male mice lost more weight and lean body mass in response to food restriction, compared to females. During refeeding, males accumulated more body fat and exhibited lower energy expenditure and glycemia, as compared to females. Additionally, female mice exhibited a higher protection against diet-induced obesity and related metabolic imbalances in comparison to males. Low dose ghrelin injection elicited higher food intake and growth hormone secretion in male mice, whereas the acute anorexigenic effect of leptin was more robust in females. However, the sex differences in the feeding responses to ghrelin and leptin were not explained by variations in the central responsiveness to these hormones nor by differences in the fiber density from arcuate nucleus neurons. Female, but not male, mice exhibited compensatory increases in hypothalamic Pomc mRNA levels in response to diet-induced obesity.Our findings revealed several sexually differentiated responses to metabolic challenges in C57BL/6 mice, highlighting the importance of taking into account sex differences in metabolic studies.
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- 2022
35. Evaluation of the immunogenicity and potency of a vaccine against Porcine Circovirus (PCV2) in mice
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Martins, Vitor Barbosa Fialho, Universidade Estadual Paulista (Unesp), and Montassier, Hélio José [UNESP]
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Vacinação ,Camundongos ,Suinocultura - Abstract
Submitted by Vitor Barbosa Fialho Martins (vitor.fialho@unesp.br) on 2022-05-03T10:11:00Z No. of bitstreams: 1 Dissertação Mestrado - Vitor_Martins_Definitivo_.pdf: 2604031 bytes, checksum: dfd722070533b517c3b05f37fcfbc203 (MD5) Approved for entry into archive by Laudicélia Martins Arantes (lm.arantes@unesp.br) on 2022-05-03T12:06:23Z (GMT) No. of bitstreams: 1 martins_vbf_me_jabo.pdf: 2604031 bytes, checksum: dfd722070533b517c3b05f37fcfbc203 (MD5) Made available in DSpace on 2022-05-03T12:06:23Z (GMT). No. of bitstreams: 1 martins_vbf_me_jabo.pdf: 2604031 bytes, checksum: dfd722070533b517c3b05f37fcfbc203 (MD5) Previous issue date: 2022-03-04 O circovirus suíno Tipo 2 (PCV2) e as doenças associadas a ele (PCVAD) geram grandes perdas para a suinocultura mundial. Além de outras medidas de prevenção para reduzir a disseminação dessa doença infecciosa, a vacinação é um dos meios mais eficientes de se controlar a circovirose nos rebanhos suínos. Há diversos tipos de vacinas comercialmente disponíveis contra o circovirus, sendo que estas devem ser atualizadas com base na evolução que os genótipos prevalentes de PCV2 têm sofrido ao longo dos anos. Assim, o presente estudo propôs a realização de dois experimentos: o primeiro teve o objetivo de comparar a resposta imune humoral anti-PCV2 em camundongos isogênicos e heterogênicos induzidas por vacinas comerciais contra este vírus e o segundo teve o objetivo de se estabelecer um modelo de avaliação da potência de vacinas contra o Circovirus Suíno em camundongos. Para a realização do Experimento 1 foram testadas duas vacinas comerciais contra o PCV2, sendo uma baseada no genótipo “a” e outra no genótipo “b” de PCV2, nas linhagens C57BL/6 e Balb/C e no estoque heterogênico Swiss. Os soros dos animais vacinados foram submetidos à mensuração de anticorpos anti-PCV2 pelo método indireto de ELISA e as médias de DOs obtidas nesse ensaio foram avaliadas e submetidas à análise estatística. Já no Experimento 2, o estoque heterogênico de camundongos Swiss foi escolhido para a realização do delineamento que contou com o mesmo esquema vacinal utilizado no Experimento 1 e foi seguido por desafio com PCV2b em três momentos. Os animais tiveram o sangue colhido e foram então eutanasiados em três momentos para avaliação da resposta imune humoral por ELISA indireto e análise da carga viral de PCV2 em sangue total e tecidos (rim, baço, pulmões e linfonodo). Os resultados demonstraram a capacidade de camundongos isogênicos e heterogênicos vacinados em gerar anticorpos contra PCV2 baseada em soroconversão e títulos end-point avaliados, além de demonstrar não haver diferença estatística (p = 0,063) entre os níveis de anticorpos dos grupos testados. Quanto ao modelo de potência, foi possível ratificar os dados obtidos no Experimento 1, com relação à resposta imune humoral e identificar que camundongos Swiss não são capazes de desenvolver sinais clínicos e apresentar viremia e carga viral em pulmões analisados pela técnica de qPCR de acordo com o delineamento utilizado. Apesar disso, conclui-se que camundongos Swiss podem ser utilizados como modelo para avaliação de resposta imune humoral e estudos de potência baseados em perfil sorológico dos animais submetidos à vacinação contra PCV2. Porcine circovirus Type 2 (PCV2) and its associated diseases (PCVAD) generate large losses for a worldwide swine industry. In addition to other preventive measures to reduce the spread of this infectious disease, vaccination is one of the most efficient ways to control circovirus in swine herds. There are several types of vaccines commercially available against circovirus, and a current update is needed because of the evolution that the prevalent PCV2 genotypes have undergone over the years. Thus, the present study proposed the performance of two experiments, the first with the objective of comparing an anti-PCV2 humoral immune response in isogenic and heterogeneous mice induced by commercial vaccines against this virus and the second with the objective of establishing a model for evaluating the potency of vaccines against the Swine Circovirus in a mouse model. To carry out Experiment 1, two commercial vaccines against PCV2 were tested, one based on the “a” genotype and the other on the “b” PCV2 genotype, on the C57BL/6 and Balb/C lines and on the Swiss heterogeneous stock. Sera from vaccinated animals were subjected to measurement of anti-PCV2 antibodies by indirect ELISA method and means of ODs were evaluated and submitted to statistical analysis. In Experiment 2, Swiss mice was chosen to carry out the design that had the same vaccination schedule used in Experiment 1 and was followed by challenge with PCV2b at three times. . The animals had their blood collected and were then euthanized at three times to assess the humoral immune response by indirect ELISA and analysis of the viral load of PCV2 in whole blood and tissues (kidney, spleen, lungs and lymph node). The results demonstrated the ability of isogenic and heterogeneous vaccinated mice to generate antibodies against PCV2 based on seroconversion and evaluated end-point titers, in addition to demonstrating that there was no statistical difference (p = 0.063) between the antibody levels of the groups tested. As for the potency model, it was possible to confirm the data obtained in Experiment 1, regarding the humoral immune response and to identify that Swiss mice are not able to develop clinical signs, and present viremia and viral load in the lungs analyzed by qPCR technique according to the design used. Despite this, it is concluded that Swiss mice can be used as a model for evaluating the humoral immune response and potency studies based on the serological profile of the animals submitted to vaccination against PCV2, with a possibility of correlation of the data obtained in this model, when compared to the target species of the vaccine evaluated.
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- 2022
36. Protective effect of β-glucan and glutamine on intestinal and immunological damage in mice induced by cytarabine (Ara-C).
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Porsani, Mariana Y. H., Paludetti, Monique, Orlando, Débora R., Peconick, Ana P., Costa, Rafael C., Oliveira, Luiz E. D., Zangeronimo, Márcio G., and Sousa, Raimundo V.
- Abstract
Copyright of Pesquisa Veterinaria Brasileira is the property of Colegio Brasileiro de Patologia Animal - CBPA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2017
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37. Efeito do estresse sobre o metabolismo glicídico de camundongos tornados obesos.
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Fernandes Gozoli, Gabriela, Manuner Zeca, Paula Regina, Calzzetta, Bruna, and Ribeiro Cesaretti, Mário Luís
- Abstract
Introduction: Sleep disorders, alone or in association with a high-calorie diet, may determine metabolic changes in the autonomic nervous system and in the hypothalamic-pituitary-adrenal axis, which may increase glycemia and produce glucose intolerance. The state of glucose intolerance usually precedes the onset of type 2 diabetes. Objective: This study aims to evaluate the effects of the association of paradoxical sleep deprivation and hypercaloric diet on the eating behavior and glucose tolerance of mice. Methods: Swiss mice were distributed into six groups: (1) control; (2) hypercaloric diet; (3) paradoxical sleep deprivation; (4) paradoxical sleep deprivation + hypercaloric diet; (5) monossodium glutamate (PSP); and (6) paradoxical sleep deprivation + monossodium glutamate. During the eight-week follow-up, food consumption was assessed and mice were weighed periodically. Sleep deprivation was performed weekly. After eight weeks, the oral glucose tolerance (TTGO) and insulin sensitivity test (TTI) were performed. At the end, heart and epididymal fat were weighed. Results: There was a significant increase in body weight in obese mice, which was associated with increased visceral fat content. All animals, obese and PSP, showed worsening in the parameters that measured the glucose metabolism, either on TTGO and TTI. There was no change in cardiac weight. Conclusion: The administration of a hypercaloric diet and paradoxical sleep deprivation determine glucose intolerance and insulin resistance, which in this work was not synergistic. [ABSTRACT FROM AUTHOR]
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- 2017
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38. REVISÃO SISTEMÁTICA SOBRE MODELOS EXPERIMENTAIS DE ASMA AGUDA E CRÔNICA INDUZIDOS COM EXTRATO DE ÁCARO DA POEIRA DOMÉSTICA.
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Santos Dutra, Moisés, Roncada, Cristian, de Souza, Rodrigo Godinho, da Cunha, Aline Andrea, and Márcio Pitrez, Paulo
- Abstract
Introduction: Asthma is a chronic disease of the lower airways with a high prevalence. Researchers worldwide have developed several experimental studies in mice with the goal of better understanding the mechanisms of the disease and testing new therapies. Mites are abundantly present in household dust, being considered the most common allergens triggering allergic asthma. This study aims to present and discuss inflammatory outcomes in the lung tissue of the mice, verify the difference between acute and chronic models of allergic asthma, time of exposure to the allergen, dose administered and its impact on research in experimental models with asthma. Methods: Literature review was performed in four databases (PubMed, Scielo, Scopus and ScienceDirect). The selected articles were first evaluated by two researchers independently, according to the inclusion criteria. Results: A total of 126 articles were separated. When the inclusion and exclusion criteria were applied, only 15 were selected. They are articles that presented different protocols of exposure to HDM. The most commonly found HDM dose was 100μg followed by 25μg, and the model type was acute. Conclusion: In the acute model, a high level of airway inflammation is observed. However, the chronic model better reproduces the characteristics of asthma in humans, bronchial hyperresponsiveness, and airway remodeling. [ABSTRACT FROM AUTHOR]
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- 2017
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39. EFEITO DA ADMINISTRAÇÃO DOS HORMÔNIOS CONTRARREGULADORES SOBRE O PERFIL GLICÊMICO DE CAMUNDONGOS SUBMETIDOS À HIPOGLICEMIA INDUZIDA POR INSULINA.
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PLATH KWIATKOWSKI, RODRIGO, DIAS ANTUNES, MATEUS, and DIAZ PEDROSA, MARIA MONTSERRAT
- Abstract
Millions of people worldwide have diabetes, and many of these, insulin therapy users who do attempt to control glucose levels in the blood. When the insulin dose is too high, your blood sugar may decrease to very low values, condition known as insulin- induced hypoglycemia (IIH). In a normal individual, hypoglycemia induced mechanisms that use counterregulatory hormones to try to counteract the hypoglycemic state of the individual, which does not adequately occurs in diabetic patients. Experimental studies on HII are numerous in normal and diabetic rats, but still scarce in mice. The objective of this investigation was to evaluate how counterregulatory hormones glucose up to oppose the action of insulin during an episode of HII in mice. The glycemic profile was monitored for 300 min after insulin administration. The counterregulatory were administered 15 min after insulin. There was no improvement in glycemic profile during the fall of blood glucose and / or during the recovery of glucose in the groups treated with counterregulatory when compared to the insulin-treated group. In the groups treated with adrenaline and growth hormone was reported a recovery of blood glucose significantly worse. How many animals came to death before the end of follow-up, it is likely that counterregulatory doses used in mice are not suitable for mice. It is suggested planning new experiments to establish these doses. [ABSTRACT FROM AUTHOR]
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- 2016
40. COMPARAÇÃO ENTRE COLETAS DE AMOSTRAS FECAIS PARA ANÁLISE MICROBIOLÓGICA EM CAMUNDONGOS ORALMENTE TOLERIZADOS
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Camilla da Paixão Duarte and Maria de Fátima Sarro da Silva
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Microbiota entérica ,enterobacterias ,ecologia bacteriana ,tolerância oral ,camundongos ,Veterinary medicine ,SF600-1100 - Abstract
A análise microbiológica de material fecal pode ser determinada a partir de coletas diretas e indiretas e posterior realização de ensaios bioquí- micos clássicos de identificação bacteriana, estando sua perfeita caracterização diretamente relacionada com a forma de coletar levando-se em consideração as características fisiológicas das espécies bacterianas a serem investigadas. O objetivo deste trabalho foi comparar a sensibilidade de dois protocolos de coletas de amostras fecais, para caracterização de amostras de Enterobacteriaceae, em duas estruturas do trato entérico de um modelo animal desenvolvido geneticamente para estudos de tolerância oral. Os resultados obtidos pelos protocolos aqui desenhados não mostraram diferenças significativas, quanto aos aspectos qualitativos e quantitativos dos isolados.
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- 2013
41. O etanercepte induz QRS de baixa tensão e disfunção autonômica em camundongos com doença de Chagas experimental
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Héctor Rodríguez-Angulo, Oscar García, Endher Castillo, Edward Cardenas, Juan Marques, and Alfredo Mijares
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Camundongos ,Doença de Chagas ,Imunogobulina G ,Sistema Nervoso ,Etanercepte ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
FUNDAMENTO: A doença de Chagas é uma doença parasitária tropical causada pelo protozoário flagelado Trypanosoma cruzi. A cardiomiopatia chagásica é caracterizada por distúrbios na regulação autonômica e na condução do potencial de ação nas fases aguda e crônica da infecção. Embora o fator de necrose tumoral alfa (TNF-α) tenha sido associadoà cardiomiopatia em modelos experimentais e em pacientes com doença de Chagas, outros relatos sugerem que o TNF-α pode exercer ações antiparasitárias durante a fase aguda da infecção. OBJETIVOS: Este estudo teve como objetivo determinar os efeitos de um blocker TNF-α solúvel, o etanercepte, em parâmetros eletrocardiográficos na fase aguda da infecção experimental com Trypanosoma cruzi. MÉTODOS: Foram feitos eletrocardiogramas em camundongos infectados não tratados e camundongos infectados que foram tratados com etanercepte 7 dias após a infecção. Os parâmetros de variabilidade onda do eletrocardiograma e frequência cardíaca foram determinados utilizando o Chart para Windows. RESULTADOS: O tratamento com etanercepte resultou em uma baixa tensão do complexo QRS e uma redução da variabilidade da frequência cardíaca em comparação com a ausência de tratamento. No entanto, os camundongos tratados apresentaram um atraso na queda da curva de sobrevivência durante a fase aguda. CONCLUSÃO: Os resultados deste estudo sugerem que, embora o tratamento com etanercepte promova a sobrevivência em camundongos infectados com uma linhagem virulenta de T. cruzi, o bloqueio do TNF-α gera um complexo de baixa tensão e disfunção autonômica durante a fase aguda da infecção. Esses resultados indicam que a mortalidade durante a fase aguda pode ser atribuída a uma resposta inflamatória sistêmica, em vez da disfunção cardíaca.
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- 2013
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42. Assessment of the cytotoxic, genotoxic, and antigenotoxic activities of Celtis iguanaea (Jacq.) in mice
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FLAVIO F.V. BORGES, THIAGO C. MACHADO, KENYA S. CUNHA, KARLA C. PEREIRA, ELSON A. COSTA, JOSE R. DE PAULA, and LEE CHEN-CHEN
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Citotoxicidade ,genotoxicidade ,camundongos ,planta medicinal ,Science - Abstract
Ethnobotanical surveys of Cerrado native plants show that leaves of Celtis iguanaea (Jacq.) Sargent (Cannabaceae), popularly known in Brazil as “esporão de galo”, are used in folk medicine for body pain, asthma, cramps, poor digestion, urinary infection, kidney dysfunctions, as well as a stimulant and diuretic. This work aimed at evaluating possible C. iguanaea aqueous leaf extract (CALE) cytotoxicity, genotoxicity, and antigenotoxicity using the mouse bone marrow micronucleous test. To assess CALE genotoxicity, Swiss mice were orally treated with three different extract concentrations (100, 300, and 500 mgkg−1). To evaluate its antigenotoxicity, the same doses were used simultaneously with a single i.p. dose of mitomycin C (MMC, 4mg.kg−1). The frequencies of micronucleated polychromatic erythrocytes (MNPCE) were evaluated 24 h and 48 h after administration except for the negative control (24 h). Genotoxicity was evaluated using the frequency of micronucleated polychromatic erythrocytes (MNPCE), whereas cytotoxicity was assessed by the polychromatic and normochromatic erythrocytes ratio (PCE/NCE). The results showed that CALE did not exhibit a significant reduction in the PCE/NCE ratio, neither a considerable increase in the frequency of MNPCE. Nonetheless, CALE reduced bone marrow toxicity (increased PCE/NCE ratio) and decreased the micronuclei frequency induced by MMC. We can conclude that CALE presented no cytotoxic and genotoxic effects, but showed antigenotoxic and anticytotoxic actions under the experimental conditions applied in this study.
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- 2013
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43. IMMUNOHISTOCHEMICAL APPROACH REVEALS LOCALIZATION OF CYSTATHIONINE-?-LYASE AND CYSTATHIONINE-ß-SYNTHETASE IN ETHANOL-INDUCED GASTRIC MUCOSA DAMAGE IN MICE
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Jand-Venes Rolim MEDEIROS, Pedro Marcos Gomes SOARES, Gerly Anne de Castro BRITO, and Marcellus Henrique Loiola Ponte de SOUZA
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Mucosa gástrica ,Cistationina-?-Sintase ,Cistationina-?-Liase ,Etanol ,Imunoistoquímica ,Camundongos ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Context Hydrogen sulphide (H2S) has been proved to be a neuromodulator and contributes to the maintenance of gastric mucosal integrity in damage caused by anti-inflammatory nonsteroidal drugs. Previously, we demonstrated that H2S synthesis is essential to gastric protection against ethanol. Objective To better understanding the role of H2S and the detailed localization of its production in both normal and injured stomach due to ethanol injection, we studied the expression of cystathionine-γ-lyase (CSE) and cystathionine-β-synthetase (CBS) isoforms in gastric mucosa of mice treated with saline or 50% ethanol. Methods Mice were treated by gavage with saline or 50% ethanol (0.5 mL/25 g). After 1 hour, mice were sacrificed, and gastric tissue was evaluated by histological and immunohistochemical analysis specific for CSE and CBS. Results We have demonstrated a non-specific expression of CBS in the normal gastric mucosa and expression of CSE occurring mainly in the parietal cells of the animals treated with ethanol. Conclusion Thus, we demonstrated that the expression of CBS appears to be constitutive and diffuse across the gastric epithelium, while the expression of CSE appears to be induced in parietal cells by damage agents such as ethanol.
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- 2013
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44. Amylin induces hypoglycemia in mice
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Luiz H. Guerreiro, Daniel DA Silva, Mauro Sola-Penna, Daniella M. Mizurini, and Luís M.T.R. Lima
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amilina ,polipeptídeo pancreático amilóide ,glicemia ,diabetes ,camundongos ,hipoglicemia ,Science - Abstract
Amylin is a 37-aminoacid pancreatic protein that exerts control over several metabolic events such as glycemia and lacticemia. Amylin has long been shown to induce increases in arterial plasma glucose. We decided to investigate whether amylin plays additional roles in the glucose metabolism. We evaluated glucose homeostasis using whole blood from the tail tip of fasting, conscious, unrestrained normal and streptozotocyn-induced diabetic mice following subcutaneous administration of mouse amylin. Subcutaneous injection of 1 μg mouse amylin caused a transient decrease in whole blood glucose in both normal and diabetic mice in the absence of insulin. The blood glucose levels were lowest approximately 2 hours after amylin administration, after that they gradually recovered to the levels of the control group. The hypoglycemic effect followed a dose-dependent response ranging from 0.1 to 50 µg / mouse. These results reveal the ability for amylin in the direct control of glycemia at low doses in the absence of insulin.
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- 2013
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45. A resposta oxidativa em corações de camundongos é modulada por background genético
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Marco Aurélio Santos-Silva, Akinori Cardozo Nagato, Eduardo Tavares Lima Trajano, Jackson Nogueira Alves, Ana Carla Balthar Bandeira, Luís Cristóvão Porto, and Frank Silva Bezerra
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Estresse Oxidativo ,Tabaco ,Fumaça ,Melhoramento Genético ,Camundongos ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
FUNDAMENTO: o tabagismo apresenta importante papel sobre as doenças cardiovasculares, entretanto permanecem pouco compreendidos os motivos pelos quais alguns seres humanos as desenvolvem e outros não. OBJETIVO: nosso objetivo foi analisar o perfil redox do coração de diferentes linhagens de camundongos após exposição à fumaça de cigarro. MÉTODOS: camundongos machos suíços (n = 10), C3H (n = 10), BALB/c (n = 10) e C57BL/6 (n = 10) foram expostos à fumaça de cigarro (12 cigarros/dia), enquanto os respectivos controles (n = 10) ao ar ambiente por 60 dias. Após sacrifício, o coração foi retirado para análises bioquímicas. RESULTADOS: embora o conteúdo de malondialdeído não tenha aumentado em nenhum grupo, a atividade da catalase diminuiu no grupo suíço (p < 0,05), BALB/c (p < 0,05) quando comparados aos respectivos grupos-controle, enquanto a mieloperoxidase diminuiu no grupo C3H (p < 0,05) e C57BL/6 (p < 0,001) quando comparados aos respectivos grupos controle. O conteúdo de glutationa reduzida diminuiu nos grupos suíço, C3H, C57BL/6 (p < 0,05) e no grupo BALB/c (p < 0,001) quando comparados com os respectivos controles. Observamos aumento do conteúdo da glutationa oxidada no grupo Suíço (p < 0,05) e diminuição nos grupos C3H (p < 0,05) e BALB/c (p < 0,001) quando comparados aos respectivos grupos-controle. A razão glutationa reduzida/ glutationa oxidada apresentou redução nos grupos suíço e C57BL/6 (p < 0.05) quando comparados aos grupos controle. CONCLUSÃO: o background genético nos camundongos pode influenciar na resposta antioxidante após a exposição à fumaça de cigarro e parece ser um fator determinante para o desequilíbrio redox no suíço e C57BL/6. Compreender as respostas antioxidantes e do background genético C3H e BALB/c podem fornecer importantes informações quanto à resistência cardíaca a fumaça de cigarro.
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- 2013
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46. Adaptações biomecânicas do osso cortical de camundongos submetidos à três diferentes modalidades de exercício
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Fernando Tadeu Trevisan Frajacomo, Maurício José Falcai, Cleverson Rodrigues Fernandes, Antonio Carlos Shimano, and Sérgio Britto Garcia
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Exercício ,Biomecânica ,Tíbia ,Camundongos ,Medicine ,Orthopedic surgery ,RD701-811 - Abstract
OBJETIVO: Comparar os efeitos adaptativos de três modalidades de exercício de impacto reduzido nas adaptações mecânicas do osso cortical. MÉTODOS: Vinte e quatro camundongos machos, espécie Balb/c (25±3g), foram divididos aleatoriamente em quatro grupos (n=6): grupo sedentário (S); grupo natação (N) realizado cinco vezes por semana, 60 minutos progressivos; grupo resistido (R) submetido ao exercício de escalada com sobrecarga progressiva, três vezes por semana; e o grupo combinado (C) que realizou os mesmos protocolos em dias alternados sendo três vezes na semana do protocolo N e duas vezes na semana protocolo R. Após o sacrifício dos animais, foi realizado o ensaio mecânico de flexão em três pontos na tíbia dos grupos experimentais para se determinar a rigidez e a força máxima de fratura. RESULTADOS: A rigidez nos grupos N (41,68 ± 10,43 N/mm) e R (41,21 ± 11,38 N/mm) foi significativamente maior comparada ao grupo S (28,48 ± 7,34 N/mm), p < 0,05. Entretanto, considerando a massa corporal final dos animais como variável, valores relativos, não houve diferença significativa nos testes biomecânicos do osso. CONCLUSÕES: Dados do presente estudo evidenciaram que o estímulo mecânico gerado pela contração muscular das modalidades isoladas de baixo impacto, grupo N e R, favoreceu o coeficiente absoluto de rigidez óssea, fato que não ocorreu na modalidade combinada, grupo C. Nível de Evidência II, Estudo Prospectivo e Comparativo.
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- 2013
47. [Untitled]
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Luiz H. Guerreiro, Daniel DA Silva, Mauro Sola-Penna, Daniella M. Mizurini, and Luís M.T.R. Lima
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amilina ,polipeptídeo pancreático amilóide ,glicemia ,diabetes ,camundongos ,hipoglicemia ,amylin ,islet associated polypeptide ,glycemia ,mice ,hypoglycemia ,Science - Abstract
Amylin is a 37-aminoacid pancreatic protein that exerts control over several metabolic events such as glycemia and lacticemia. Amylin has long been shown to induce increases in arterial plasma glucose. We decided to investigate whether amylin plays additional roles in the glucose metabolism. We evaluated glucose homeostasis using whole blood from the tail tip of fasting, conscious, unrestrained normal and streptozotocyn-induced diabetic mice following subcutaneous administration of mouse amylin. Subcutaneous injection of 1 μg mouse amylin caused a transient decrease in whole blood glucose in both normal and diabetic mice in the absence of insulin. The blood glucose levels were lowest approximately 2 hours after amylin administration, after that they gradually recovered to the levels of the control group. The hypoglycemic effect followed a dose-dependent response ranging from 0.1 to 50 µg / mouse. These results reveal the ability for amylin in the direct control of glycemia at low doses in the absence of insulin.Amilina é uma proteína de 37 aminoácidos que exerce controle sobre diversos eventos metabólicos, tais como glicemia e lacticemia. Tem sido mostrado que amilina induz aumento da glicemia plasmática arterial. Nós decidimos investigar se amilina exerce função adicional na regulação do metabolismo de glicose. Nós avaliamos a homeostase de glicose empregando sangue total da ponta da cauda de camundongos normais e diabéticos em condição livre de anestésico e não-confinados. Injeção subcutânea de 1 μg por camundongo causou o decréscimo transiente na glicemia tanto em camundongos normais quanto em diabéticos na ausência de administração de insulina. Os níveis de glicose atingiram o mínimo em aproximadamente 2 horas após administração de amilina, seguido por gradual restauração a níveis do grupo controle. O efeito hipoglicemiante demonstrou ser dose-dependente na faixa de 0,1 to 50 μg / camundongo. Esses resultados revelam a abilidade de amilina exercer direto controle sobre a glicemia em baixas dose na ausência de insulina.
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- 2013
48. Propriedades físicoquímicas e avaliação da toxicidade aguda do extrato etanólico padronizado a 70% das folhas de Mikania glomerata (Asteraceae)
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L.C.L.R. Santana, M.R.M. Brito, G.F. Sousa, and R.M. Freitas
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Mikania glomerata ,Asteraceae ,Toxicidade Aguda ,Camundongos ,Pharmacy and materia medica ,RS1-441 ,Botany ,QK1-989 - Abstract
Mikania glomerata (Asteraceae) é bastante utilizada na medicina popular devido às suas ações broncodilatadora, antiasmática, expectorante e antitussígena. O objetivo do presente estudo foi determinar propriedades físicoquímicas do pó obtido a partir das folhas de M. glomerata, bem como avaliar a toxicidade em camundongos após tratamento agudo com doses repetidas do extrato etanólico padronizado a 70% preparado durante 30 dias consecutivos. No estudo das propriedades físicoquímicas fez-se a determinação da densidade bruta e de compactação, do teor de cinzas totais, do teor de umidade e da granulometria. De acordo com os resultados obtidos o pó pode ser usado na formulação de uma forma farmacêutica sólida, uma vez que suas propriedades físico-químicas são compatíveis com o desenvolvimento desse tipo de formulação. Na segunda parte do estudo foi determinada a dose letal 50% (DL50) em camundongos, e na análise morfológica macroscópica dos principais órgãos e avaliada a toxicidade aguda com doses repetidas em parâmetros bioquímicos e hematológicos de camundongos. Os resultados sugerem que o extrato etanólico padronizado a 70% pode ser usado de forma segura, uma vez que apresentou um valor para a DL50 (~3000 mg kg-1) que pode ser classificado na categoria nociva, e não produziu nenhuma alteração morfológica nos principais órgãos e em parâmetros bioquímicos e hematológicos de camundongos.
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- 2013
- Full Text
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49. Oxidative stress gene expression profile in inbred mouse after ischemia/reperfusion small bowel injury Perfil da expressão gênica do estresse oxidativo em camundongos isogênicos após lesão de isquemia e reperfusão intestinal
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Paulo Roberto Bertoletto, Adauto Tsutomu Ikejiri, Frederico Somaio Neto, José Carlos Chaves, Roberto Teruya, Eduardo Rodrigues Bertoletto, Murched Omar Taha, and Djalma José Fagundes
- Subjects
Estresse Oxidativo ,Antioxidantes ,Expressão Gênica ,Isquemia ,Reperfusão ,Reação em Cadeia da Polimerase em Tempo Real ,Camundongos ,Oxidative Stress ,Antioxidants ,Gene expression ,Ischemia ,Reperfusion ,Real-Time Polymerase Chain Reaction ,Mice ,Surgery ,RD1-811 - Abstract
PURPOSE: To determine the profile of gene expressions associated with oxidative stress and thereby contribute to establish parameters about the role of enzyme clusters related to the ischemia/reperfusion intestinal injury. METHODS: Twelve male inbred mice (C57BL/6) were randomly assigned: Control Group (CG) submitted to anesthesia, laparotomy and observed by 120min; Ischemia/reperfusion Group (IRG) submitted to anesthesia, laparotomy, 60min of small bowel ischemia and 60min of reperfusion. A pool of six samples was submitted to the qPCR-RT protocol (six clusters) for mouse oxidative stress and antioxidant defense pathways. RESULTS: On the 84 genes investigated, 64 (76.2%) had statistic significant expression and 20 (23.8%) showed no statistical difference to the control group. From these 64 significantly expressed genes, 60 (93.7%) were up-regulated and 04 (6.3%) were down-regulated. From the group with no statistical significantly expression, 12 genes were up-regulated and 8 genes were down-regulated. Surprisingly, 37 (44.04%) showed a higher than threefold up-regulation and then arbitrarily the values was considered as a very significant. Thus, 37 genes (44.04%) were expressed very significantly up-regulated. The remained 47 (55.9%) genes were up-regulated less than three folds (35 genes - 41.6%) or down-regulated less than three folds (12 genes - 14.3%). CONCLUSION: The intestinal ischemia and reperfusion promote a global hyper-expression profile of six different clusters genes related to antioxidant defense and oxidative stress.OBJETIVO: Determinar o perfil de expressão dos genes associados com estresse oxidativo e contribuir para estabelecer parâmetros sobre o papel das familias de enzimas relacionadas com a lesão de isquemia / reperfusão intestinal. MÉTODOS: Doze camundongos machos isogênicos (C57BL/6) foram distribuídos aleatoriamente: Grupo Controle (CG) submetido à laparotomia anestesia, e observado por 120min; Grupo isquemia/reperfusão (IRG) submetido à anestesia, laparotomia, 60min de isquemia do intestino delgado e 60min de reperfusão. Um pool dos seis camundongos de cada grupo foi submetido ao protocolo de qPCR-RT (seis famílias) para o estresse oxidativo e defesa antioxidante. RESULTADOS: Dos 84 genes investigados, 64 (76,2%) tiveram expressão estatística significante e 20 (23,8%) não apresentaram diferença estatística com o grupo controle. Dos 64 genes expressos de forma significante, 60 (93,7%) foram hiper-expressos e 04 (6,3%) foram hipo-expressos. Do grupo sem expressão estatisticamente significante, 12 genes foram hiper e 8 genes foram hipo-expressos. Surpreendentemente, 37 (44,04%) apresentaram expressão três maior que o limiar de normalidade e arbitrariamente os valores foram considerados como altamente significantes. Assim, 37 genes (44,04%) foram hiper-expressos de modo muito significante. Nos demais, 47 (55,9%) dos genes foram hiper-expressos menos de três vezes (35 genes - 41,6%) ou hipo-expressos menos de três vezes(12 genes - 14,3%). CONCLUSÃO: A isquemia e reperfusão intestinal promoveu um perfil de hiper-expressão global das seis familias de genes relacionados com estresse oxidativo antioxidante e defesa antioxidante.
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- 2012
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50. Repurposing topical triclosan for cutaneous leishmaniasis: Preclinical efficacy in a murine Leishmania (L.) amazonensis model
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Noemi Nosomi Taniwaki, Juliana Mariotti Guerra, Andre G. Tempone, Maiara M. Romanelli, Juliana T. Mesquita, Cristiana de Melo Trinconi Trinconi Cm, Silvia R. B. Uliana, and Juliana Quero Reimão
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Antiprotozoal Agents ,Leishmaniasis, Cutaneous ,Pharmacology ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Cutaneous leishmaniasis ,In vivo ,Drug Discovery ,medicine ,Animals ,Humans ,Amastigote ,Leishmania ,Mice, Inbred BALB C ,Miltefosine ,biology ,business.industry ,Drug Repositioning ,Leishmaniasis ,medicine.disease ,Antimicrobial ,biology.organism_classification ,Triclosan ,chemistry ,030220 oncology & carcinogenesis ,CAMUNDONGOS ,business ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Leishmaniasis remains an important neglected tropical infection caused by the protozoan Leishmania and affects 12 million people in 98 countries. The treatment is limited with severe adverse effects. In the search for new therapies, the drug repositioning and combination therapy have been successfully applied to neglected diseases. The aim of the present study was to evaluate the in vitro and in vivo anti-Leishmania (Leishmania) amazonensis potential of triclosan, an approved topical antimicrobial agent used for surgical procedures. in vitro phenotypic studies of drug-treated parasites were performed to evaluate the lethal action of triclosan, accompanied by an isobolographic ex-vivo analysis with the association of triclosan and miltefosine. The results showed that triclosan has activity against L. (L.) amazonensis intracellular amastigotes, with a 50% inhibitory concentration of 16 μM. By using fluorescent probes and transmission electron microscopy, a pore-forming activity of triclosan toward the parasite plasma membrane was demonstrated, leading to depolarization of the mitochondrial membrane potential and reduction of the reactive oxygen species levels in the extracellular promastigotes. The in vitro interaction between triclosan and miltefosine in the combination therapy assay was classified as additive against intracellular amastigotes. Leishmania-infected mice were treated with topical triclosan (1% base cream for 14 consecutive days), and showed 89% reduction in the parasite burden. The obtained results contribute to the investigation of new alternatives for the treatment of cutaneous leishmaniasis and suggest that the coadministration of triclosan and miltefosine should be investigated in animal models.
- Published
- 2020
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