15 results on '"Campos, Patrícia M. B. G. Maia"'
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2. Characterization of aging hair and its influence in quality of life
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Gabarra, Marcella A. L., Favaretto, Gabriela, Martini, Ana Paula M., and Campos, Patrícia M. B. G. Maia
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integumentary system ,MEDICINE ,ENVELHECIMENTO ,AGEING ,WELLNESS ,QUALIDADE DE VIDA ,BEM-ESTAR ,CABELO ,QUALITY OF LIFE ,otorhinolaryngologic diseases ,MEDICINA ,HAIR ,DERMATOLOGIA ,sense organs ,DERMATOLOGY - Abstract
There are few studies that characterize the non-pigmented hair compared to pigmented hair. Furthermore, it is important to evaluate how the natural process of aging hair influences quality of life in women. For this purpose, a questionnaire was applied to evaluate the impact of greying hair and the appearance alterations on women´s self-esteem. The satisfaction level of volunteers regarding their hair structure and conditions such as shine, strength and smoothness were also evaluated. Mechanical properties, such as elasticity and traction to rupture were measured using Universal test equipment. Scanning electron microscopy (SEM) was used to measure the hair surface morphological conditions. A large majority of the volunteers indicated that the beginning of greying hair can negatively influence their quality of life. They also reported that the primary changes of their hair were the frizziness, dryness and loss of shine, increased loss and breakage and thinning. Morphological evaluation showed that the non-pigmented hair cuticles were thinner than pigmented hair. The adherence of the cuticle scales was more pronounced in the non-pigmented hair .Finally, this study showed the influence of the appearance of grey hair on quality of life in women as well as the morphological alterations resulting from the greying processes., Poucos são os estudos que caracterizam o cabelo não pigmentado em comparação com o cabelo pigmentado. Além disso, é importante avaliar como esse processo natural de envelhecimento capilar influencia na qualidade de vida das mulheres. Para isso, foi aplicado um questionário para avaliar o impacto do aparecimento dos cabelos grisalhos e as alterações que estes causam na auto-estima. Foi avaliado o nível de satisfação das voluntárias com a estrutura dos cabelos e suas condições, tais como brilho, força e suavidade. Foram avaliadas as propriedades mecânicas, tais como elasticidade e tração à ruptura com o equipamento Universal. As condições morfológicas pormicroscopia eletrônica de varredura A maioria das voluntárias relataram que o aparecimento dos cabelos grisalhos influencianegativamente na qualidade de vida e que as principais alterações dos cabelos eram o aumento do frizz, ressecamento, perda de brilho, quebra dos fios e afinamento capilar A avaliação morfológica mostrou que as cutículas do cabelo não pigmentados eram mais finas que as do pigmentado. A adesão das cutículas foi mais pronunciada no cabelo não pigmentado. Por fim, este estudo mostrou a influência do aparecimento de cabelos grisalhos na qualidade de vida da mulher e as alterações morfológicas que estes processos podem causar nos fios.
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- 2015
3. Desenvolvimento de formulações cosméticas contendo o polímero glucan de Mandioca (Manihot esculenta): Estabilidade e análise sensorial
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Manço, Luisa M., Mercúrio, Daiane Garcia, Melo, Maísa O., and Campos, Patrícia M. B. G. Maia
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PELE ,CASE STUDIES ,ESTUDOS DE CASO ,PRODUTOS COSMÉTICOS ,CIÊNCIAS FARMACÊUTICAS ,POLYMERS ,COSMETICS ,SKIN ,POLÍMEROS ,PHARMACEUTICAL SCIENCES - Abstract
The aim of this study was the development, rheological behaviour determination, and sensory analysis of cosmetic formulations containing glucan biopolymer (Manihot esculenta), a tensor agent that was proposed to produce an immediate lifting and smoothing effect. For this purpose, formulations were developed and supplemented or not with 4 % of hydrolysed Manihot esculenta tuber extract and submitted to preliminary stability tests. These formulations were evaluated in terms of rheological behaviour over 90 days. Sensory analysis was carried out through a research with 20 cosmetic consumers who answered a questionnaire regarding their perception to the cosmetic qualities. The formulations presented pseudoplastic behavior and were considered stable in the physical stability studies, with the exception of the gel formulation based on Ammonium Acryloyldimethyltaurate/VP Copolymer. The formulations were well evaluated in the sensory parameters. The gel formulations based on Polyacrylamide, C13- 14 Isoparaffin, and Laureth-7 were stable and presented the best sensory profile in some evaluated parameters, such as spreadability, smoothness and skin moisturizing, and can be considered an appropriate vehicle for formulations containing hydrolysed Manihot esculenta tuber extract., O objetivo desse trabalho é o desenvolvimento, avaliação do comportamento reológico e análise sensorial de formulações contendo o biopolímero glucan (Manihot esculenta). Para tal, foram desenvolvidas formulações que foram adicionadas, ou não, de 4 % do extrato hidrolisado da raíz de Manihot esculenta e submetidas a testes preliminares de estabilidade. Essas formulações foram submetidas a análises de comportamento reológico durante 90 dias. A análise sensorial foi realizada em um grupo de 20 consumidoras de cosméticos. As formulações foram aplicadas na região do antebraço das voluntárias e foi aplicado um questionário sobre as percepções das qualidades cosméticas. As formulações apresentaram comportamento pseudoplástico e foram consideradas estáveis nos testes de estabilidade física, exceto a formulação em gel a base de Ammonium Acryloyldimethyltaurate/VP Copolymer. As formulações foram bem avaliadas em seus parâmetros sensoriais. As formulações em gel baseadas em Polyacrylamide (and) C13-14 Isoparaffin (and) Laureth-7 foram estáveis e apresentaram o perfil sensorial mais agradável e, considerando os testes de estabilidade física realizados, pode-se concluir que é um veículo apropriado para formulações contendo o extrato hidrolisado da raíz de Manihot esculenta.
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- 2015
4. Formulações para os cuidados do cabelo contendo óleo de argan: desenvolvimento, estabilidade e perfil de textura
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Delsin, Stefânia Duz and Campos, Patrícia M. B. G. Maia
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CABELO ,CASE STUDIES ,ESTUDOS DE CASO ,PRODUTOS COSMÉTICOS ,HAIR ,CIÊNCIAS FARMACÊUTICAS ,COSMETICS ,PHARMACEUTICAL SCIENCES - Abstract
O objetivo deste estudo foi desenvolver e avaliar a estabilidade e textura de formulações cosméticas para os cuidados dos cabelos contendo óleo de argan. Formulações de shampoos, condicionadores e leave on foram desenvolvidas com ou sem (veículo) óleo de argan nas concentrações de 0,1, 2,0 e 2,0% (a/a), respectivamente. As formulações foram armazenadas à temperatura ambiente (25°C), 37°C e 45°C, por 28 dias e submetidas à testes de espalhabilidade e de textura utilizando um texturômetro. Foram avaliados os parâmetros de “Work of Shear” e consistência. Os resultados mostraram que as formulações foram estáveis e as formulações com óleo de argan mostraram melhor espalhabilidade quando comparada com o veículo. Este é um efeito desejável pois formulações cosméticas que apresentam baixos valores de “Work of Shear” são mais bem aceitas por painéis sensoriais. Além disso, não houve diferença entre os condicionadores, com ou sem óleo de argan, em termos de perfil de textura e a formulação leave on contendo óleo de argan mostrou um aumento da consistência em relação ao o veículo. Por fim, as formulações contendo o óleo de argan mostraram um melhor perfil de textura, e portanto, este é um potencial ingrediente para utilização em formulações para os cuidados dos cabelos., The aim of this study was to develop hair care cosmetic formulations containing argan oil to evaluate the stability and texture profile of these formulations. Shampoos, conditioners and leave-in formulations were developed with or without (vehicle) argan oil in concentrations of 0.1, 2.0 and 2.0% (w/w) respectively. The formulations were stored at room temperature (25°C), 37°C and 45°C for a period of 28 days and submitted to spreadability and texture tests using the Texture Analyser. For this purpose, work of shear and consistency parameters were determined. The results have shown that all formulations were stable and the argan oil-based formulations had a better spreadability when compared with the vehicle. This is a desirable effect, once cosmetic formulations which presents a lower values of work of shear are better accepted by cosmetic sensory panels. In addition, no difference was found between conditioners with or without argan oil in terms of texture profile, and leave-informulation with argan oil showed an increased consistency when compared with the vehicle. Finally, formulations with argan oil showed better the texture profile, therefore, it is a potential ingredient for use in hair care formulations.
- Published
- 2015
5. Antioxidant activity of Matricaria chamomilla L. extract and clinical efficacy of cosmetic formulations containing this extract and its isolated compounds
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Nóbrega, Ananda T., Wagemaker, Tais A. L., and Campos, Patrícia M. B. G. Maia
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PELE ,ANTIOXIDANTS ,ANTIOXIDANTES ,PLANTAS ,PLANTS ,CIÊNCIAS FARMACÊUTICAS ,FARMACOLOGIA ,PHARMACOLOGY ,SKIN ,PHARMACEUTICAL SCIENCES - Abstract
A aplicação tópica de antioxidantes provou ser eficaz em proteger a pele contra danos oxidativos. O extracto de Matricaria chamomilla L. tem sido amplamente utilizado em formulações cosméticas. Assim, o objectivo deste estudo foi avaliar o potencial antioxidante do extracto de camomila, bem como a eficácia clínica na hidratação e nas propriedades mecânicas da pele. A actividade antioxidante do extracto de camomila foi avaliada por quimioluminescência (IC50 = 0,14 ug / mL). As formulações foram suplementadas com a α -bisabolol de fração mássica 0,5 cg/g ou com extracto glicólico de Matricaria chamomilla L. de fração mássica 5,0 cg/g ou com 0,01 cg/g de apigenina e aplicados na parte interna do antebraço e na cara de 25 mulheres. A fisiologia da pele foi avaliada antes e após 2 horas (aplicação única) e 2 e 4 semanas de aplicação diária. Depois de uma única aplicação, todas as formulações aumentaram a água do estrato córneo, mas apenas as formulações com α -bisabolol e extracto de camomila reduziram a PTEA . A formulação com o extracto de camomila mostrou o resultado mais marcante na redução PTEA (27%). No entanto, após 2 - e 4- semanas de aplicação, apenas a formulação contendo o extracto aumentou o a água no estrato córneo. O extracto de camomila, foi eficaz na neutralização de radicais livres e, portanto, apresenta potencial em formulações cosméticas com este propósito., Topical application of antioxidants has proven to be effective in protecting the skin against oxidative damage. Matricaria chamomilla L. extract has been used in cosmetic formulations. The aim of this study was to evaluate the antioxidant potential of chamomile extract as well as the clinical efficacy on skin hydration and mechanical properties of skin. The antioxidant activity of chamomile extract was evaluated by chemiluminescence (IC50 = 0.14 μg/mL). Stable formulations were supplemented with 0,5 cg/g α-bisabolol or cg/gwith 5,0 of Matricaria chamomile glycolic extract or with 0,01 cg/g of apigenin and applied on the volar forearm and face of 25 female subjects. Skin physiology was assessed before and after 2 hours (single application) and after a 2- and 4-week period of daily application. After a single application, all formulations increased the stratum corneum water content but only α-bisabolol and chamomile extract formulations reduced TEWL. The formulation with chamomile extract has shown the most pronounced result in the reduction of TEWL (27%). However, after 2- and 4-week application, only the extract formulation increased stratum corneum water content compared with the vehicle. Chamomile extract was effective in neutralizing free radicals and therefore presents an interesting potential in cosmetic formulations for this purpose.
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- 2014
6. Rheology, clinical efficacy and sensorial of a silicone-based formulation containing pearl extract
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Marcon, Ana Flora V. S., Wagemaker, Tais A. L., and Campos, Patrícia M. B. G. Maia
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PELE ,integumentary system ,CASE STUDIES ,RHEOLOGY ,ESTUDOS DE CASO ,PRODUTOS COSMÉTICOS ,CIÊNCIAS FARMACÊUTICAS ,REOLOGIA ,COSMETICS ,eye diseases ,SKIN ,PHARMACEUTICAL SCIENCES - Abstract
In traditional Chinese medicine, pearl extract is believed to nourish skin and prevent the aging process. Thus, the aim of this study was to evaluate rheological characteristics, immediate effects on skin, and sensory properties of a silicone-based cosmetic formulation containing pearl extract. Formulations containing pearl extract or not were submitted to physical stability assays by determinations of its rheological behavior during 28 days storage. Once confirmed the formulation’s stability, they were applied on the volar forearm of 15 healthy female to evaluate skin properties by non-invasive techniques. After a single application, formulations increased the stratum corneum water content. Once applied on the volar forearm for sensory analysis, the volunteer’s majority noted that both formulations improved all the sensory skin attributes, but only the pearl extract formulation has shown the higher scores for all attributes. Results suggested that the silicone-based formulation supplemented with the pearl extract could combine unique sensory properties and effectiveness to better meet consumer’s demand., Na medicina tradicional chinesa, acredita-se que o extracto de pérola pode nutrir a pele e prevenir o processo de envelhecimento. Assim, o objectivo deste estudo foi avaliar as características reológicas, os efeitos imediatos sobre a pele e as propriedades sensoriais de uma formulação cosmética à base de silicone contendo extracto de pérola. As formulações contendo extrato de pérola ou não foram foram submetidas a ensaios de estabilidade física por determinação do comportamento reológico durante 28 dias de armazenamento. Uma vez que as formulações se mostraram estáveis, elas foram aplicadas na região interna do antebraço de 15 indivíduos saudáveis do sexo feminino para avaliar as condições da pele usando técnicas não-invasivas. Após uma única aplicação, as formulações aumentaram o conteúdo de água do estrato córneo. Quando as formulações foram aplicadas no antebraço para a análise sensorial, a maioria dos voluntários notaram que ambas as formulações melhoraram todos os atributos sensoriais da pele, mas que apenas a formulação com extrato de pérola mostrou as maiores notas para todos eles. Os resultados sugerem que a formulação à base de silicone adicionada de extracto de pérolas pode combinar propriedades sensoriais únicas e eficácia para melhor atender a demanda dos consumidores.
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- 2014
7. Unsaponifiable matter from oil of green coffee beans: cosmetic properties and safety evaluation
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Wagemaker, Tais A. L., primary, Campos, Patrícia M. B. G. Maia, additional, Fernandes, Ana Sofia, additional, Rijo, Patrícia, additional, Nicolai, Marisa, additional, Roberto, Amílcar, additional, Rosado, Catarina, additional, Reis, Catarina, additional, Rodrigues, Luis M., additional, Carvalho, Cássia Regina Limonta, additional, Maia, Nilson Borlina, additional, and Guerreiro Filho, Oliveiro, additional
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- 2016
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8. Stability and clinical efficacy of moisturizing cosmetic formulations containing Vitamins C and E
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Capitani, Raphael Dário, Mercúrio, Daiane Garcia, Camargo Júnior, Flávio Bueno de, and Campos, Patrícia M. B. G. Maia
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PELE ,PRODUTOS COSMÉTICOS ,VITAMINS ,HYDRATION ,VITAMINAS ,CIÊNCIAS FARMACÊUTICAS ,COSMETICS ,HIDRATAÇÃO ,SKIN ,PHARMACEUTICAL SCIENCES - Abstract
A adição de diferentes substâncias activas em produtos cosméticos, por exemplo, vitaminas e seus derivados, tem sido bastante frequente. Desta forma, é fundamental avaliar a estabilidade e eficácia de formulações cosméticas que contêm estes ingredientes. O objectivo deste trabalho foi o de avaliar a estabilidade física e a eficácia clínica da hidratação formulações cosméticas com base em pantenol, e contendo tetraisopalmitato de ascorbilo e a-tocoferol. Por isso, as formulações foram desenvolvidas com pantenol (FA), pantenol e tetraisopalmitato de ascorbilo e a-tocoferol (FBC), que foi submetido à análise do comportamento reológico, caracterizando-as como estável. Em seguida, foram subjectivamente e objectivamente analisados quanto à eficácia clínica em 25 voluntários por avaliação sensorial e técnicas de bioengenharia cutânea, em termos de teor de água do estrato córneo, a perda de água transepidérmica (TEWL) e microrrelevo da pele, antes e depois de 3 horas de aplicação . As formulações estudadas apresentaram estabilidade aceitável de acordo com os aspectos físicos considerados. Nos estudos de eficácia clínica, ambas as formulações melhoraram significativamente a hidratação da pele e reduziram a TEWL, e também demonstraram uma melhoria no microrrelevo da pele, mas estes resultados não foram estatisticamente significativos. FBC recebeu notas superiores FA por análise sensorial, com melhoria significativa da suavidade da pele, além de ser preferido em intenção de compra., The addition of different active substances in cosmetics, e.g. vitamins and their derivatives, has been quite frequent. In this way, it is fundamental to evaluate the stability and efficacy of cosmetic formulations containing these ingredients. The objective of this work was to evaluate the physical stability and the clinical efficacy of moisturizing cosmetic formulations based on panthenol, and containing ascorbyl tetraisopalmitate and a-tocopherol acetate. Formulations were developed with panthenol (FA), panthenol and ascorbyl tetraisopalmitate and a-tocopherol acetate (FBC). The latter was submitted to rheological behavior analysis and all were characterized as stable. Next, they were subjectively and objectively analyzed for clinical efficacy in 25 volunteers. Sensory evaluation and biophysical and skin imaging techniques were used to measure stratum corneum water content, transepidermal water loss (TEWL), and skin microrelief. These were carried out before application of the formulations and then after 3 hours. The formulations studied showed acceptable stability according to the physical aspects under consideration. In the clinical efficacy studies, both formulations significantly improved skin hydration and reduced TEWL. There was also improvement in skin microrelief but these results were not statistically significant. FBC received higher scores than FAin the sensory analysis as there was a significant improvement in the skin's smoothness, besides being preferred in purchase intent.
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- 2013
9. Evaluation of antioxidant and antimicrobial activities of green coffee oil in cosmetic formulations
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Wagemaker, Tais A. L., Fernandes, Ana Sofia, Campos, Patrícia M. B. G. Maia, Rodrigues, Luís Monteiro, and Rijo, Patrícia
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ANTIMICROBIANOS ,ANTIOXIDANTS ,PRODUTOS COSMÉTICOS ,ANTIOXIDANTES ,ANTIMICROBIAL ,CIÊNCIAS FARMACÊUTICAS ,COSMETICS ,PHARMACEUTICAL SCIENCES - Abstract
O óleo de café verde (OCV) e formulações cosméticas que contendo 2,5, 5, 10 e 15% de óleo foram avaliados por métodos in vitro quanto às suas atividades antioxidante e antimicrobiana. O OCV e as suas formulações demostraram baixa actividade antioxidante, avaliada pelo método DPPH (42% do OCV foi equivalente a 0,002% de BHT). Não se observou atividade antimicrobiana para o OCV e as suas formulações contra Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Bacilus subtilis, Propionibacterium acnes e Candida albicans, utilizando o método de difusão em poço. Embora o OCV seja utilizado há muitos anos em formulações cosméticas, ainda são necessários mais estudos para apoiar adequadamente a utilidade do óleo de café em produtos para cuidado da saúde da pele e em cosméticos., Green coffee oil (GCO) and cosmetic formulations containing 2.5, 5, 10 and 15% of this oil were evaluated for in vitro antioxidant and antimicrobial activities. The oil and the formulations containing this oil showed low antioxidant activity evaluated by the DPPH method (42% of GCO was equivalent to 0.002% of BHT). No antimicrobial activity was found for GCO and formulations against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Bacilus subtilis, Propionibacterium acnes and Candida albicans, using the well diffusion method. Although GCO has been used for many years in cosmetic formulations, further studies are still needed to duly support the usefulness of GCO in skin healthcare and cosmetic products.
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- 2013
10. Estudo da variação do pH da pele humana exposta à formulação cosmética acrescida ou não das vitaminas A, E ou de ceramida, por metodologia não invasiva
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Leonardi, Gislaine Ricci, primary, Gaspar, Lorena Rigo, additional, and Campos, Patrícia M. B. G. Maia, additional
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- 2002
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11. In Vitro Antioxidant and In Vivo Photoprotective Effects of an Association of Bioflavonoids with Liposoluble Vitamins.
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Campos, Patrícia M. B. G. Maia, Gianeti, Mirela D., Kanashiro, Alexandre, Lucisano-Valim, Yara M., and Gaspar, Lorena R.
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- 2006
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12. Avaliação das propriedades sensoriais de uma formulação cosmética contendo óleo de café verde
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Wagemaker, Tais A. L., Catarina Rosado, Andrade, Jirrah P., Fernandes, Ana Sofia, Rijo, Patrícia, Campos, Patrícia M. B. G. Maia, and Rodrigues, Luís Monteiro
- Subjects
PELE ,CASE STUDIES ,ESTUDOS DE CASO ,PRODUTOS COSMÉTICOS ,CIÊNCIAS FARMACÊUTICAS ,COSMETICS ,SKIN ,PHARMACEUTICAL SCIENCES - Abstract
O óleo de café verde (OCV) é um ingrediente bastante conhecido com propriedades cosméticas como: manter a hidratação da pele, melhorar o fator de proteção solar e manter a função barreira da pele. Assim, o objectivo deste estudo foi avaliar a influência da adição de uma quantidade considerável de OCV (15%) nas propriedades sensoriais de uma formulação cosmética. O painel sensorial consistiu de 19 voluntários com idades entre 19 e 43 anos. Os atributos sensoriais foram avaliadas em uma região definida de 25 cm2 na parte interna do antebraço. Os voluntários foram instruídos a avaliar as propriedades sensoriais que eles sentiam para cada formulação imediatamente e 5 minutos após a aplicação. As formulações mostraram quase a mesma percepção entre os voluntários. A maioria dos voluntários percebeu a pele suave e hidratada após a aplicação das formulações. No entanto, a percepção de um resíduo oleoso sobre a pele foi o principal efeito da formulação contendo OCV. Assim, podemos concluir que a quantidade total de OCV utilizado mostrou propriedades interessantes para aplicação em peles secas ou cremes noturnos, uma vez que foi capaz de deixar uma película oleosa sobre a pele., Green coffee oil (GCO) is a well-known ingredient with cosmetic properties such as: maintaining skin hydration, improving the sun protection factor and maintaining the skin barrier function. Thus, the aim of this study was to evaluate the impact of the addition of a considerable amount of GCO (15%) on the sensory properties of a cosmetic formulation. The sensory panel consisted of nineteen volunteers aged between 19 and 43 years old. Sensory attributes were assessed on a 25 cm2 defined region on the internal side of the forearm. The volunteers were instructed to evaluate the sensory properties that they felt each formulation demonstrated, immediately after application and then after a further 5 minutes. The formulations had almost the same perceived effect among the volunteers. The majority of volunteers noted their skin as soft and moisturized after application of the formulations. However, the perception of an oily residue on the skin was the main effect of the formulation containing GCO. Thus, we can conclude that the total amount of GCO used, revealed interesting properties for use in dry skin or night creams, since it was able to leave an oily film on skin.
13. Skin characterization and immediate effects of different dermocosmetic treatments in French and Brazilian skin.
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Salomão Calixto L, Picard C, Savary G, and Campos PMBGM
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- Adult, Brazil, Elasticity drug effects, Elasticity physiology, France, Geography, Humans, Skin chemistry, Skin Aging physiology, Treatment Outcome, Viscosity drug effects, Water Loss, Insensible drug effects, Water Loss, Insensible physiology, Young Adult, Bleaching Agents administration & dosage, Cosmetic Techniques, Skin drug effects, Skin Aging drug effects, Skin Cream administration & dosage
- Abstract
Background: All over the world, people face the same skin problems. However, their skin characteristics are different. Thus, it is a challenge to prescribe treatments that will be effective on different skin types. Therefore, it is very important to consider the skin biology when indicating a dermocosmetic treatment., Objectives: To assess skin biophysical parameters in French and Brazilian subjects and to verify the efficacy of four dermocosmetic treatments in these populations., Methods: Five test areas were defined on each volar forearm of the eighteen Brazilian and eighteen French participants using a randomized design. Biophysical measurements in terms of skin hydration, skin barrier function, skin brightness, and skin viscoelasticity were performed before and after 60 minutes of treatment., Results: Skin biophysical differences between populations were found. French skin has been shown to be more hydrated regarding epidermal mechanic properties and stratum corneum water content and more radiant when compared with Brazilian skin. However, it showed more signs of cutaneous aging and fatigue effects on skin. The Brazilian skin showed better skin barrier function. In addition, the treatments were effective in both populations., Conclusions: Despite the differences found in French and Brazilian skin, the proposed dermocosmetic treatments showed effective in both populations., (© 2019 Wiley Periodicals, Inc.)
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- 2020
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14. Development and photoprotective effect of a sunscreen containing the antioxidants Spirulina and dimethylmethoxy chromanol on sun-induced skin damage.
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Souza C and Campos PMBGM
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- Adult, Chromans analysis, Humans, Middle Aged, Single-Blind Method, Sun Protection Factor, Antioxidants analysis, Skin radiation effects, Spirulina, Sunlight adverse effects, Sunscreening Agents chemistry
- Abstract
The literature claims that incorporation of antioxidants into sunscreens provides additional skin photoprotection by scavenging free radicals formed due to sun radiation, but there are limited in vivo studies that support this hypothesis. This study aims to examine whether addition of antioxidants to a broad-spectrum sunscreen increases its photoprotective effect in real-use conditions. Sunscreen formulations composed of stable UV filters (Tinosorb® S, Tinosorb® M, Uvinul® APlus, and Uvinul® T150) alone or in combination with antioxidants (Spirulina and dimethylmethoxy chromanol-loaded solid lipid nanoparticles) were developed and their appearance, odor, rheological behavior, Sun Protection Factor (SPF), and UVA protection were analyzed. Next, it was conducted a 3-month, single-blind clinical study with 44 healthy subjects (30-50years). Before and 28, 54, and 84days after twice-daily self application of the sunscreens on the face, the stratum corneum water content, transepidermal water loss, dermis echogenicity, and skin elasticity and pigmentation were measured. At the end of the study period, the volunteers answered a questionnaire containing terms related to sensory characteristics of the formulations. All formulations were stable and exhibited non-Newtonian and pseudoplastic behavior, in vivo SPF 30, and good UVA protection. Antioxidant supplementation to the sunscreen formulation significantly improved the skin pigmentation, the collagen degradation on the dermis and thereby the skin net elasticity after 84days of treatment compared to the sunscreen alone. Concerning safety, all formulations were considered non-irritant according to the sensorial analyses, whose results agreed with the clinical study findings., (Copyright © 2017. Published by Elsevier B.V.)
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- 2017
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15. In vitro antioxidant activity and in vivo efficacy of topical formulations containing vitamin C and its derivatives studied by non-invasive methods.
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Campos PM, Gonçalves GM, and Gaspar LR
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- Administration, Topical, Dose-Response Relationship, Drug, Humans, Skin drug effects, Skin Absorption drug effects, Skin Aging drug effects, Antioxidants administration & dosage, Ascorbic Acid administration & dosage, Cosmetics administration & dosage, Dermatologic Agents administration & dosage, Reactive Oxygen Species metabolism, Skin metabolism, Skin Absorption physiology, Skin Aging physiology
- Abstract
Background/purpose: Vitamins C and its derivatives, mainly due to their antioxidant properties, are being used in cosmetic products to protect and to reduce the signs of ageing. However, there are no studies comparing the effects of vitamin C [ascorbic acid (AA)] and its derivatives, magnesium ascorbyl phosphate (MAP) and ascorbyl tetra-isopalmitate (ATIP), when vehiculated in topical formulations, mainly using objective measurements, which are an important tool in clinical efficacy studies. Thus, the objective of this study was to determine the in vitro antioxidant activity of AA and its derivatives, MAP and ATIP, as well as their in vivo efficacy on human skin, when vehiculated in topical formulations., Methods: The study of antioxidant activity in vitro was performed with an aqueous and a lipid system. The in vivo methodology consisted of the application of these formulations on human volunteers' forearm skin and the analysis of the skin conditions after 4-week period daily applications in terms of transepidermal water loss (TEWL), stratum corneum moisture content and viscoelasticity using a Tewameter, Corneometer and Cutometer, respectively., Results: In vitro experiments demonstrated that in an aqueous system, AA had the best antioxidant potential, and MAP was more effective than ATIP, whereas in the lipid system ATIP was more effective than MAP. In in vivo studies, all formulations enhanced stratum corneum moisture content after a 4-week period daily applications when compared with baseline values; however, only the formulation containing AA caused alterations in TEWL values. The formulations containing MAP caused alterations in the viscoelastic-to-elastic ratio, which suggested its action in the deeper layers of the skin., Conclusion: AA and its derivates presented an in vitro antioxidant activity but AA had the best antioxidant effect. In in vivo efficacy studies, only the formulation containing AA caused alterations in TEWL values and the formulation containing MAP caused alterations in the viscoelastic-to-elastic ratio. This way, vitamin C derivatives did not present the same effects of AA on human skin; however, MAP showed other significant effect-improving skin hydration, which is very important for the normal cutaneous metabolism and also to prevent skin alterations and early ageing.
- Published
- 2008
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