16 results on '"Campos, Angélica Cristine de Almeida"'
Search Results
2. Prevalence of bat viruses associated with land-use change in the Atlantic Forest, Brazil
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Loh, Elizabeth H., Nava, Alessandra, Murray, Kris A., Olival, Kevin J., Guimarães, Moisés, Shimabukuro, Juliana, Zambrana-Torrelio, Carlos, Fonseca, Fernanda R., de Oliveira, Daniele Bruna Leal, Campos, Angélica Cristine de Almeida, Durigon, Edison L., Ferreira, Fernando, Struebig, Matthew J., Daszak, Peter, Loh, Elizabeth H., Nava, Alessandra, Murray, Kris A., Olival, Kevin J., Guimarães, Moisés, Shimabukuro, Juliana, Zambrana-Torrelio, Carlos, Fonseca, Fernanda R., de Oliveira, Daniele Bruna Leal, Campos, Angélica Cristine de Almeida, Durigon, Edison L., Ferreira, Fernando, Struebig, Matthew J., and Daszak, Peter
- Abstract
Introduction: Bats are critical to maintaining healthy ecosystems and many species are threatened primarily due to global habitat loss. Bats are also important hosts of a range of viruses, several of which have had significant impacts on global public health. The emergence of these viruses has been associated with land-use change and decreased host species richness. Yet, few studies have assessed how bat communities and the viruses they host alter with land-use change, particularly in highly biodiverse sites. Methods: In this study, we investigate the effects of deforestation on bat host species richness and diversity, and viral prevalence and richness across five forested sites and three nearby deforested sites in the interior Atlantic Forest of southern Brazil. Nested-PCR and qPCR were used to amplify and detect viral genetic sequence from six viral families (corona-, adeno-, herpes-, hanta-, paramyxo-, and astro-viridae) in 944 blood, saliva and rectal samples collected from 335 bats. Results: We found that deforested sites had a less diverse bat community than forested sites, but higher viral prevalence and richness after controlling for confounding factors. Viral detection was more likely in juvenile males located in deforested sites. Interestingly, we also found a significant effect of host bat species on viral prevalence indicating that viral taxa were detected more frequently in some species than others. In particular, viruses from the Coronaviridae family were detected more frequently in generalist species compared to specialist species. Discussion: Our findings suggest that deforestation may drive changes in the ecosystem which reduce bat host diversity while increasing the abundance of generalist species which host a wider range of viruses.
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- 2022
3. Atypical Prolonged Viral Shedding With Intra-Host SARS-CoV-2 Evolution in a Mildly Affected Symptomatic Patient
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Cunha, Marielton dos Passos, Vilela, Ana Paula Pessoa, Molina, Camila Vieira, Acuña, Stephanie Maia, Muxel, Sandra Marcia, Barroso, Vinícius de Morais, Baroni, Sabrina, Gomes de Oliveira, Lilian, Angelo, Yan de Souza, Peron, Jean Pierre Schatzmann, Góes, Luiz Gustavo Bentim, Campos, Angélica Cristine de Almeida, and Minóprio, Paola
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SARS-CoV-2 ,intra-host evolution ,viruses ,coronavirus ,Medicine ,General Medicine ,prolonged RNA viral shedding ,immune response ,Original Research - Abstract
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is caused by a respiratory virus with a wide range of manifestations, varying from asymptomatic to fatal cases, with a generally short outcome. However, some individuals present long-term viral shedding. We monitored 38 individuals who were mildly affected by the SARS-CoV-2 infection. Out of the total studied population, three (7.9%) showed atypical events regarding the duration of positivity for viral RNA detection. In one of these atypical cases, a previously HIV-positive male patient presented a SARS-CoV-2 RNA shedding and subgenomic RNA (sgRNA) detected from the upper respiratory tract, respectively, for 232 and 224 days after the onset of the symptoms. The SARS-CoV-2 B.1.1.28 lineage, one of the most prevalent in Brazil in 2020, was identified in this patient in three serial samples. Interestingly, the genomic analyses performed throughout the infectious process showed an increase in the genetic diversity of the B.1.1.28 lineage within the host itself, with viral clearance occurring naturally, without any intervention measures to control the infection. Contrasting widely spread current knowledge, our results indicate that potentially infectious SARS-CoV-2 virus might be shed by much longer periods by some infected patients. This data call attention to better adapted non-pharmacological measures and clinical discharge of patients aiming at preventing the spread of SARS-CoV-2 to the population.
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- 2021
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4. One-step protocol for amplification of near full-length cDNA of the rabies virus genome
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Campos, Angélica Cristine de Almeida, Melo, Fernando Lucas, Romano, Camila Malta, Araujo, Danielle Bastos, Cunha, Elenice Maria Sequetin, Sacramento, Débora Regina Veiga, de Andrade Zanotto, Paolo Marinho, Durigon, Edison Luiz, and Favoretto, Silvana Regina
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- 2011
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5. Detection, quantification and genetic variability of M. hyopneumoniae from healthy and symptomatic swine (English)
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Pulgarón, Yaima Burgher, Marques, Lucas Miranda, Campos, Angélica Cristine de Almeida, Rodriguez, Joan Peña, Márquez, Odaylin Plasencia, Ortiz, Arianna Duque, Rivero, Evelyn Lobo, and Timenetsky, Jorge
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Quantificação ,Healthy ,PCR ,Enzootic pneumonia ,VNTR ,Quantification ,M. hyopneumoniae ,detection ,Pneumonia enzoótica ,Saudável - Abstract
Mycoplasma hyopneumoniae is the causative agent of the Porcine Enzootic Pneumonia. However, this mycoplasma can be detected in healthy and symptomatic pigs, that difficults the conclusion for the etiology of this disease. In the present study we aimed to detect, quantify and do molecular analyses of M. hyopneumoniae strains in respiratory clinical samples recovered from healthy pigs and from those with pneumonia or other respiratory symptoms. The analytical sensitivity and specificity of PCR assays directed to Mollicutes detection and porcine mycoplasmas identification in clinical samples were evaluated. The identification of M. hyopneumoniae in the samples was performed using different molecular approaches, Multiplex PCR, Real Time PCR and Multilocus Variable-Number Tandem-Repeat amplification. Molecular characterization of the strains was achieved by determining and comparing the VNTR copy number directly in the samples. The highest number of samples positive to M. hyopneumoniae was identified by the multilocus VNTR amplification assay using labeled primers, followed by capillary electrophoresis. The highest concentration of M. hyopneumoniae was detected in pneumonic lungs (2, 3 * 108 genome copies /mL). The VNTR copy number analysis demonstrated that despite the high genetic variability of the M. hyopneumoniae strains, predominant strains in the swine farms could be identified by means of the VNTR copy number analysis of P97R1 and P146R3. (English) Molecular differences among Mycoplasma hyopneumoniae strains present in pneumonic lungs of swine have been largely studied. However, no comparative studies concerning the strains present in apparently healthy pigs have been carried out. This study aimed to detect, quantify and perform molecular analysis of M. hyopneumoniae strains in pig lungs with and without pneumonic lesions. The detection of M. hyopneumoniae was performed using multiplex PCR (YAMAGUTI, 2008), real-time PCR (STRAIT et al., 2008) and multiple VNTR amplification (VRANCKX et al., 2011). Molecular characterization of the strains was achieved by analysis of the VNTR copy number in P97R1, P146R3, H2R1 and H4. M. hyopneumoniae was detected in samples from healthy and pneumonic pigs and the amount of M. hyopneumoniae positive samples detected varied with the type of assay. The greater number of positive samples was identified by the multiple VNTR amplification combined with capillary electrophoresis. Using real-time PCR, 4.9*104 M. hyopneumoniae genome copies/mL was detected in apparently healthy lungs. A mean quantity of 3.9*106 M. hyopneumoniae genome copies/mL was detected in pneumonic lungs. The analysis of VNTR copy number demonstrated a high genetic variability of the M. hyopneumoniae strains present in apparently healthy and pneumonic lungs. Strains having 3 VNTR copy number in P97R1, were detected only in pneumonic lungs and strains having 40 and 43 VNTR copy number in P146R3 were detected only in apparently healthy lungs. Despite the genetic variability of M. hyopneumoniae, predominant strains in the swine farms could be identified. As diferenças moleculares entre as estirpes de Mycoplasma hyopneumoniae presentes em pulmões de suínos com pneumonia tem sido estudadas. Porém, estudos comparativos relativos as estirpes presentes nos suínos aparentemente saudáveis não foram levados a cabo. O objetivo do estudo foi a detecção, quantificação e analise molecular de M. hyopneumoniae nos pulmões suínos com e sem lesões pneumônicas. Para a detecção de M. hyopneumoniae usaramse o PCR Multiplo (YAMAGUTI, 2008), o PCR a Tempo Real (STRAIT et al., 2008) e a amplificação de múltiplo VNTR (VRANCKX et al., 2011). A caracterização molecular das estirpes foi realizada mediante a análise do número de copias de VNTR em P97R1, P146R3, H2R1 e H4. O M. hyopneumoniae foi detectado em amostras de suínos saudáveis e pneumônicos e a quantidade de M. hyopneumoniae nas amostras positivas variou com o tipo de ensaio. O maior número de amostras positivas foi identificado pela amplificação de múltiplas VNTR combinado com a eletroforese de capilares. Usando o PCR a Tempo Real, 4.9*104 copias de genoma/mL de M. hyopneumoniae foram detectadas em pulmões aparentemente saudáveis. Uma quantidade média de 3.9*106 copias de genoma/mL de M. hyopneumoniae foi detectada em pulmões pneumônicos. A análise do número de copias de VNTR demonstrou uma elevada variabilidade.
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- 2015
6. Genetic diversity of bats coronaviruses in the Atlantic Forest hotspot biome, Brazil
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Góes, Luiz Gustavo Bentim, Campos, Angélica Cristine de Almeida, Carvalho, Cristiano de, Ambar, Guilherme, Queiroz, Luzia Helena, Cruz-Neto, Ariovaldo Pereira, Munir, Muhammad, Durigon, Edison Luiz, Góes, Luiz Gustavo Bentim, Campos, Angélica Cristine de Almeida, Carvalho, Cristiano de, Ambar, Guilherme, Queiroz, Luzia Helena, Cruz-Neto, Ariovaldo Pereira, Munir, Muhammad, and Durigon, Edison Luiz
- Abstract
Bats are notorious reservoirs of genetically-diverse and high-profile pathogens, and are playing crucial roles in the emergence and re-emergence of viruses, both in human and in animals. In this report, we identified and characterized previously unknown and diverse genetic clusters of bat coronaviruses in the Atlantic Forest Biome, Brazil. These results highlight the virus richness of bats and their possible roles in the public health.
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- 2016
7. Genetic diversity of bats coronaviruses in the Atlantic Forest hotspot biome, Brazil
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Góes, Luiz Gustavo Bentim, primary, Campos, Angélica Cristine de Almeida, additional, Carvalho, Cristiano de, additional, Ambar, Guilherme, additional, Queiroz, Luzia Helena, additional, Cruz-Neto, Ariovaldo Pereira, additional, Munir, Muhammad, additional, and Durigon, Edison Luiz, additional
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- 2016
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8. Detection, quantification and genetic variability of Mycoplasma hyopneumoniae from apparently healthy and pneumonic swine
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Pulgarón, Yaima Burgher, primary, Marques, Lucas Miranda, additional, Campos, Angélica Cristine de Almeida, additional, Rodriguez, Joan Peña, additional, Márquez, Odaylin Plasencia, additional, Ortiz, Arianna Duque, additional, Rivero, Evelyn Lobo, additional, and Timenetsky, Jorge, additional
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- 2015
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9. Molecular characterization of rabies virus isolated from non-haematophagous bats in Brazil
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Albas, Avelino, Campos, Angélica Cristine de Almeida, Araujo, Danielle Bastos, Rodrigues, Camila Seabra, Sodré, Miriam Martos, Durigon, Edison Luiz, and Favoretto, Silvana Regina
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Antigenic characterization ,Raiva ,Caracterização antigênica ,Rabies ,Bats ,Caracterização genética ,Morcegos ,Genetic characterization - Abstract
INTRODUCTION: Rabies is an important zoonosis that causes thousands of deaths worldwide each year. Although the terrestrial cycle, mainly transmitted by dogs, is controlled in Brazil, the aerial cycle remains a serious public health issue, besides the economic problem. In the aerial cycle, the haematophagous bat Desmodus rotundus is the main source of infection, where several different species of non-haematophagous bats can be infected and can transmit the virus. METHODS: The aim of this work was to study the epidemiological pattern of rabies using antigenic characterization with monoclonal antibodies and genetic characterization by reverse-transcriptase polymerase chain reaction followed by sequencing and phylogenetic analysis of non-haematophagous bats' and herbivorous animals' central nervous system samples from the western region of the State of São Paulo, Brazil. RESULTS: From 27 samples, 3 antigenic variants were identified: AgV-3, AgV-4, and AgV-6; and from 29 samples, 5 different clusters were identified, all belonging to the rabies virus species. CONCLUSIONS: Although only non-haematophagous bats were evaluated in the studied region, the majority of samples were from antigenic and genetic variants related to haematophagous bats Desmodus rotundus. Samples from the same antigenic variant were segregated in more than one genetic cluster. This study demonstrated the diversity of rabies virus genetic lineages presented and circulating in non-haematophagous bats in the studied region. INTRODUÇÃO: A raiva é uma importante zoonose responsável por milhares de mortes anualmente em todo o mundo. Embora o ciclo silvestre, onde os cães são os principais transmissores esteja controlado no Brasil, o ciclo aéreo, onde o morcego hematófago Desmodus rotundus é o principal transmissor e diversas espécies de morcegos não hematófagos podem se infectar e transmitir o vírus, permanence como um importante problema econômico e de saúde pública. MÉTODOS: O objetivo deste trabalho foi a caracterização antigênica por meio da utilização de anticorpos monoclonais e a caracterização genética por meio da reação em cadeia pela polimerase pela transcriptase reversa seguida de análise filogenética em morcegos não hematófagos e animais domésticos herbívoros provenientes da região oeste do Estado de São Paulo. RESULTADOS: A análise antigênica de 27 amostras determinou três variantes distintas: Agv-3, AgV-4 e AgV-6; a análise genética de 29 amostras identificou 5 diferentes grupos, todos pertencentes a espécie Rabies virus. CONCLUSÕES: Ainda que apenas amostras de morcegos não hematófagos tenham sido analisadas, a maioria das variantes antigênicas e genéticas identificadas na região estava relacionada com a variante mantida pelos morcegos hematófagos Desmodus rotundus. Amostras de uma mesma variante antigênica segregaram em mais de um clado genético. Este estudo demonstrou a diversidade de linhagens genéticas do vírus da raiva presentes e circulantes em morcegos não hematófagos na região estudada.
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- 2011
10. Molecular characterization of rabies virus isolated from non-haematophagous bats in Brazil
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Albas, Avelino, primary, Campos, Angélica Cristine de Almeida, additional, Araujo, Danielle Bastos, additional, Rodrigues, Camila Seabra, additional, Sodré, Miriam Martos, additional, Durigon, Edison Luiz, additional, and Favoretto, Silvana Regina, additional
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- 2011
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11. First report of rabies infection in bats, Molossus molossus, Molossops neglectus and Myotis riparius in the city of São Paulo, State of São Paulo, southeastern Brazil
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Rosa, Adriana Ruckert da, primary, Kataoka, Ana Paula de Arruda Geraldes, additional, Favoretto, Silvana Regina, additional, Sodré, Miriam Martos, additional, Trezza Netto, José, additional, Campos, Angélica Cristine de Almeida, additional, Durigon, Edison Luiz, additional, and Martorelli, Luzia Fátima Alves, additional
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- 2011
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12. Characterization of rabies virus isolated from a colony of Eptesicus furinalis bats in Brazil
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Almeida, Marilene Fernandes de, primary, Favoretto, Silvana R., additional, Martorelli, Luzia F. Alves, additional, Trezza-Netto, José, additional, Campos, Angélica Cristine de Almeida, additional, Ozahata, Carlos H., additional, Sodré, Miriam Martos, additional, Kataoka, Ana Paula A. G., additional, Sacramento, Débora R. Veiga, additional, and Durigon, Edison L., additional
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- 2011
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13. Estudo genético da variante do vírus da raiva mantida por populações do morcego hematófago Desmodus rotundus.
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Campos, Angélica Cristine de Almeida, primary
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14. Comparison between ectodomain and G2 region of G glycoprotein for genotyping of HRSV
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Campos, Angélica Cristine de Almeida, primary, Durigon, Edison Luiz, additional, Leal, Andréa Lima, additional, Silva, Tereza Souza, additional, Bosso, Patrícia Alves Ramos, additional, Moraes, Claudia Trigo Pedroso, additional, Oliveira, Danielle Bruna Leal, additional, Lima, Hildener Nogueira, additional, Vieira, Sandra Elisabete, additional, Botosso, Viviane Fongaro, additional, Zanotto, Paolo Marinho de Andrade, additional, and Stewien, Klaus Eberhard, additional
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- 2007
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15. Experimental infection of the bat tick Carios fonsecai (Acari: Ixodidae) with the rabies virus.
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Favoretto SR, de Almeida MF, Martorelli LF, Aires CC, Labruna MB, Kataoka AP, Campos AC, Sacramento DR, Durigon EL, and Koprowski H
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- Animals, Fluorescent Antibody Technique, Mice, RNA, Viral analysis, Reverse Transcriptase Polymerase Chain Reaction, Chiroptera virology, Ixodidae virology, Rabies virus immunology
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Introduction: This study assessed the viability of the rabies virus in the argasid tick Carios fonsecai following experimental infection., Methods: The mouse inoculation test (MIT), fluorescent antibody test (FAT) and polymerase chain reaction (PCR) were used. The rabies virus was administered to ticks via the intra-coelomic route, and the ticks were sacrificed at different time points., Results: The inoculated ticks were negative for rabies according to the MIT. Ticks macerated with rabies virus were positive according to the MIT and FAT. All of the tick lots tested by PCR were positive. CONCLUSIONS The rabies virus became unviable shortly after its inoculation into tick bodies. Ticks are not likely to play an important role in the epidemiology of rabies.
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- 2013
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16. Molecular epidemiology of the SH (small hydrophobic) gene of human respiratory syncytial virus (HRSV), over 2 consecutive years.
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Lima HN, Botosso VF, Oliveira DB, Campos AC, Leal AL, Silva TS, Bosso PA, Moraes CT, Filho CG, Vieira SE, Gilio AE, Stewien KE, and Durigon EL
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- Child, Preschool, Cluster Analysis, Genotype, Humans, Infant, Molecular Epidemiology, Molecular Sequence Data, Nasopharynx virology, Phylogeny, RNA, Viral genetics, Respiratory Syncytial Virus, Human isolation & purification, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Polymorphism, Genetic, Respiratory Syncytial Virus Infections epidemiology, Respiratory Syncytial Virus Infections virology, Respiratory Syncytial Virus, Human classification, Respiratory Syncytial Virus, Human genetics, Retroviridae Proteins, Oncogenic genetics
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Human respiratory syncytial virus (HRSV) strains were isolated from nasopharyngeal aspirates collected from 965 children between 2004 and 2005, yielding 424 positive samples. We sequenced the small hydrophobic protein (SH) gene of 117 strains and compared them with other viruses identified worldwide. Phylogenetic analysis showed a low genetic variability among the isolates but allowed us to classify the viruses into different genotypes for both groups, HRSVA and HRSVB. It is also shown that the novel BA-like genotype was well segregated from the others, indicating that the mutations are not limited to the G gene., (Copyright © 2011 Elsevier B.V. All rights reserved.)
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- 2012
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