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2. Robotic assisted versus laparoscopic surgery for deep endometriosis: a meta-analysis of current evidence

Author

Pavone, M, Baroni, A, Campolo, F, Goglia, M, Raimondo, D, Carcagni, A, Akladios, C, Marescaux, J, Fanfani, F, Scambia, G, Ianieri, M, Pavone M., Baroni A., Campolo F., Goglia M., Raimondo D., Carcagni A., Akladios C., Marescaux J., Fanfani F., Scambia G., Ianieri M. M., Pavone, M, Baroni, A, Campolo, F, Goglia, M, Raimondo, D, Carcagni, A, Akladios, C, Marescaux, J, Fanfani, F, Scambia, G, Ianieri, M, Pavone M., Baroni A., Campolo F., Goglia M., Raimondo D., Carcagni A., Akladios C., Marescaux J., Fanfani F., Scambia G., and Ianieri M. M.

Abstract

Endometriosis is a benign inflammatory onco-mimetic disease affecting 10–15% of women in the world. When it is refractory to medical treatments, surgery may be required. Usually, laparoscopy is the preferred approach, but robotic surgery has gained popularity in the last 15 years. This study aims to evaluate the safety and efficacy of robotic-assisted laparoscopic surgery (RAS) versus conventional laparoscopic surgery (LPS) in the treatment of endometriosis. This study adheres to PRISMA guidelines and is registered with PROSPERO. Studies reporting perioperative data comparing RAS and LPS surgery in patients with endometriosis querying PubMed, Google Scholar and ClinicalTrials.gov were included in the analysis. The Quality Assessment of Diagnostic Accuracy Studies 2 tool (QUADAS-2) was used for the quality assessment of the selected articles. Fourteen studies were identified, including 2709 patients with endometriosis stage I-IV for the meta-analysis. There were no significant differences between RAS and LPS in terms of intraoperative and postoperative complications, conversion rate and estimated blood loss. However, patients in the RAS group have a longer operative time (p < 0.0001) and longer hospital stay (p = 0.020) than those in the laparoscopic group. Robotic surgery is not inferior to laparoscopy in patients with endometriosis in terms of surgical outcomes; however, RAS requires longer operative times and longer hospital stay. The benefits of robotic surgery should be sought in the easiest potential integration of robotic platforms with new technologies. Prospective studies comparing laparoscopy to the new robotic systems are desirable for greater robustness of scientific evidence.

Published
2024

3. Anatomical-based classification of dorsolateral parametrectomy for deep endometriosis. Correlation with surgical complications and functional outcomes: A single- center prospective study

Author

Ianieri, M, Alesi, M, Querleu, D, Ercoli, A, Chiantera, V, Carcagni, A, Campolo, F, Greco, P, Scambia, G, Ianieri M. M., Alesi M. V., Querleu D., Ercoli A., Chiantera V., Carcagni A., Campolo F., Greco P., Scambia G., Ianieri, M, Alesi, M, Querleu, D, Ercoli, A, Chiantera, V, Carcagni, A, Campolo, F, Greco, P, Scambia, G, Ianieri M. M., Alesi M. V., Querleu D., Ercoli A., Chiantera V., Carcagni A., Campolo F., Greco P., and Scambia G.

Abstract

Objective: To evaluate complication rate and functional outcomes of nerve-sparing parametrectomy for deep endometriosis in relation to the extension of the surgical procedure, based on recognizable anatomical landmarks. Methods: This was a prospective single-center study including all patients undergoing parametrectomy for deep endometriosis from September 2020 to June 2023 at our tertiary center. Dorsolateral parametrectomies were divided into parametrectomies medial to the presacral fascia and cranial to the medial rectal artery (superficial parametrectomy), and parametrectomies in which one of the two landmarks was overcome during the surgical procedure, leading to the excision of tissue lateral to the presacral fascia (deep parametrectomy type 1, or DP1) or caudal to the medial rectal artery (DP2). Finally, we used the hypogastric fascia as landmark to define type 3 deep parametrectomy (DP3), when the procedure was deeply lateral to the fascia. Results: Bladder voiding deficit occurred in 9.7% of cases, with higher rates in DP2 (20.8%) and DP3 (30%) groups. Regarding postoperative gastrointestinal function, our data showed a significant improvement over time in all groups, with the exception of DP2; instead an improvement in postoperative bladder function was only shown in DP3. Parametrectomy was not associated with a simultaneous improvement in sexual function expressed with the female sexual function index, in any of the four groups. Conclusion: Our classification constitutes a concrete approach for comparing, in a standardized way, the complications and functional outcomes of parametrectomy, which, even if carried out by expert surgeons, demonstrates a non-negligible rate of bladder voiding deficit.

Published
2024

4. Totally intracorporeal colorectal anastomosis (TICA) versus classical mini-laparotomy for specimen extraction, after segmental bowel resection for deep endometriosis: a single-center experience

Author

Ianieri, M, De Cicco Nardone, A, Greco, P, Carcagni, A, Campolo, F, Pacelli, F, Scambia, G, Santullo, F, Ianieri M. M., De Cicco Nardone A., Greco P., Carcagni A., Campolo F., Pacelli F., Scambia G., Santullo F., Ianieri, M, De Cicco Nardone, A, Greco, P, Carcagni, A, Campolo, F, Pacelli, F, Scambia, G, Santullo, F, Ianieri M. M., De Cicco Nardone A., Greco P., Carcagni A., Campolo F., Pacelli F., Scambia G., and Santullo F.

Abstract

Purpose: The surgical approach to bowel endometriosis is still unclear. The aim of the study is to compare TICA to conventional specimen extractions and extra-abdominal insertion of the anvil in terms of both complications and functional outcomes. Methods: This is a single-center, observational, retrospective study conducted enrolling symptomatic women underwent laparoscopic excision of deep endometriosis with segmental bowel resection between September 2019 and June 2022. Women who underwent TICA were compared to classical technique (CT) in terms of intra- and postoperative complications, moreover, functional outcomes relating to the pelvic organs were assessed using validated questionnaires [Knowles-Eccersley-Scott-Symptom (KESS) questionnaire and Gastro-Intestinal Quality of Life Index (GIQLI)] for bowel function. Pain symptoms were assessed using Visual Analogue Scale (VAS) scores. Results: The sample included 64 women. TICA was performed on 31.2% (n = 20) of the women, whereas CT was used on 68.8% (n = 44). None of the patients experienced rectovaginal, vesicovaginal, ureteral or vesical fistula, or ureteral stenosis and uroperitoneum, and in no cases was it necessary to reoperate. Regarding the two surgical approaches, no significant difference was observed in terms of complications. As concerns pain symptoms at 6-month follow-up evaluations on stratified data, except for dysuria, all VAS scales reported showed significant reductions between median values, for both surgery interventions. As well, significant improvements were further observed in KESS scores and overall GIQLI. Only the GIQLI evaluation was significantly smaller in the TICA group compared to CT after the 6-month follow-up. Conclusions: We did not find any significant differences in terms of intra- or post-operative complications compared TICA and CT, but only a slight improvement in the Gastro-Intestinal Quality of Life Index in patients who underwent the CT compared to the TICA technique.

Published
2024

6. Carcinoid Syndrome: Preclinical Models and Future Therapeutic Strategies

Author

Vitale, G., Carra, S., Alessi, Y., Campolo, F., Pandozzi, C., Zanata, I., Colao, A., and Faggiano, A.

Subjects
Organic Chemistry, carcinoid syndrome, General Medicine, neuroendocrine tumors, pharmacological treatment, preclinical models, serotonin, xenograft, Catalysis, Computer Science Applications, Settore MED/13 - Endocrinologia, Inorganic Chemistry, Physical and Theoretical Chemistry, Molecular Biology, Spectroscopy
Abstract

Carcinoid syndrome represents a debilitating paraneoplastic disease, caused by the secretion of several substances, occurring in about 10–40% of patients with well-differentiated neuroendocrine tumors (NETs). The main signs and symptoms associated with carcinoid syndrome are flushing, diarrhea, hypotension, tachycardia, bronchoconstriction, venous telangiectasia, dyspnea and fibrotic complications (mesenteric and retroperitoneal fibrosis, and carcinoid heart disease). Although there are several drugs available for the treatment of carcinoid syndrome, the lack of therapeutic response, poor tolerance or resistance to drugs are often reported. Preclinical models are indispensable tools for investigating the pathogenesis, mechanisms for tumor progression and new therapeutic approaches for cancer. This paper provides a state-of-the-art overview of in vitro and in vivo models in NETs with carcinoid syndrome, highlighting the future developments and therapeutic approaches in this field.

Published
2023

7. Characterization of circulating immune cells and correlation with Tie2/Angiopoietins level in well differentiated neuroendocrine gastroenteropancreatic tumors: a cross-sectional analysis

Author

Sesti, F., primary, Puliani, G., additional, Feola, T., additional, Campolo, F., additional, Sciarra, F., additional, Hasenmajer, V., additional, Lenzi, A., additional, Faggiano, A., additional, Isidori, A. M., additional, Venneri, M. A., additional, and Giannetta, E., additional

Published
2022
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10. Hysteroembryoscopy and hysteroscopic uterine evacuation of early pregnancy loss: A feasible procedure in selected cases

Author

Catena, Ursula, D'Ippolito, Silvia, Campolo, F, Dinoi, G, Lanzone, Antonio, Scambia, Giovanni, Catena, U, D'Ippolito, S (ORCID:0000-0002-6160-0558), Lanzone, A (ORCID:0000-0003-4119-414X), Scambia, G (ORCID:0000-0003-2758-1063), Catena, Ursula, D'Ippolito, Silvia, Campolo, F, Dinoi, G, Lanzone, Antonio, Scambia, Giovanni, Catena, U, D'Ippolito, S (ORCID:0000-0002-6160-0558), Lanzone, A (ORCID:0000-0003-4119-414X), and Scambia, G (ORCID:0000-0003-2758-1063)

Abstract

Hysteroscopic uterine evacuation of early pregnancy loss using tissue removal devices seems to be a safe and feasible procedure in selected cases. The hysteroscopic approach allows the precise localisation of the gestational sac inside the uterine cavity. The endoscopic approach allows one to perform hysteroembryoscopy before uterine evacuation and this technique appears to be more accurate than dilatation & curettage for fetal chromosome karyotyping, with lower maternal cell contamination. This "under vision" procedure may reduce retained products of conception rates and risk of intrauterine adhesions formation.

Published
2022

11. The polymorphism L412F in TLR3 inhibits autophagy and is a marker of severe COVID-19 in males

Author

Croci, S., Venneri, M. A., Mantovani, S., Fallerini, C., Benetti, E., Picchiotti, N., Campolo, F., Imperatore, F., Palmieri, M., Daga, S., Gabbi, C., Montagnani, F., Beligni, G., Farias, T. D. J., Carriero, M. L., Di Sarno, L., Alaverdian, D., Aslaksen, S., Cubellis, M. V., Spiga, O., Baldassarri, M., Fava, F., Norman, P. J., Frullanti, E., Isidori, A. M., Amoroso, A., Mari, F., Furini, S., Mondelli, M. U., GEN-COVID multicenter study, Chiariello, M., Renieri, A., it/ gen-covid) Mirella Bruttini, Meloni I. GEN-COVID Multicenter Study (https://sites. google. com/dbm. unisi., Rossella, Tita, Sara, Amitrano, Anna Maria Pinto, Maria Antonietta Mencarelli, Caterina Lo Rizzo17, Valentina, Perticaroli1, 2, 17, Massimiliano, Fabbiani19, Barbara, Rossetti19, Giacomo, Zanelli2, Elena, Bargagli20, Laura, Bergantini20, Miriana, D’Alessandro20, Paolo, Cameli20, David, Bennett20, Federico, Anedda21, Simona, Marcantonio21, Sabino, Scolletta21, Federico, Franchi21, Maria Antonietta Mazzei22, Susanna, Guerrini22, Edoardo, Conticini23, Luca, Cantarini23, Bruno, Frediani23, Danilo, Tacconi24, Chiara, Spertilli24, Marco, Feri25, Alice, Donati25, Raffaele, Scala26, Luca, Guidelli26, Genni, Spargi27, Marta, Corridi27, Cesira, Nencioni28, Leonardo, Croci28, Gian Piero Caldarelli29, Maurizio, Spagnesi30, Paolo, Piacentini30, Maria, Bandini30, Elena, Desanctis30, Silvia, Cappelli30, Anna, Canaccini31, Agnese, Verzuri31, Valentina, Anemoli31, Agostino, Ognibene32, Alessandro, Pancrazi32, Maria, Lorubbio32, Massimo, Vaghi33, Antonella D’Arminio Monforte34, Esther, Merlini34, Federica Gaia Miraglia34, Raffaele, Bruno35, Marco, Vecchia35, Serena, Ludovisi37, Massimo, Girardis38, Sophie, Venturelli38, Marco, Sita38, Andrea, Cossarizza39, Andrea, Antinori40, Alessandra, Vergori40, Arianna, Emiliozzi40, Stefano, Rusconi41, Matteo, Siano42, Arianna, Gabrieli42, Agostino, Riva41, Daniela, Francisci4344, Elisabetta, Schiaroli4344, Francesco, Paciosi44, Pier Giorgio Scotton45, Francesca, Andretta45, Sandro, Panese45, Renzo, Scaggiante47, Francesca, Gatti48, Saverio Giuseppe Parisi48, Melania degli Antoni49, Isabella, Zanella51, Matteo Della Monica52, Carmelo, Piscopo52, Mario, Capasso53, 54, 55, Roberta, Russo53, Immacolata, Andolfo53, Achille, Iolascon53, Giuseppe, Fiorentino55, Massimo, Carella56, Marco, Castori56, Filippo, Aucella57, Pamela, Raggi58, Carmen, Marciano58, Rita, Perna58, Matteo, Bassetti59, Antonio Di Biagio59, Maurizio, Sanguinetti61, Luca, Masucci61, Serafina, Valente63, Marco, Mandalà64, Alessia, Giorli64, Lorenzo, Salerni64, Patrizia, Zucchi65, Pierpaolo, Parravicini65, Elisabetta Menatti66 Stefano Baratti45, Tullio, Trotta67, Ferdinando, Giannattasio67, Gabriella, Coiro67, Fabio, Lena68, Coviello69, Domenico A., Cristina, Mussini70, Giancarlo, Bosio71, Enrico, Martinelli71, Sandro, Mancarella72, Luisa, Tavecchia72, Mary Ann Belli72, Lia, Crotti73, 74, 75, Gianfranco, Parati73, Marco, Gori77, Maurizio, Sanarico79, Stefano, Ceri80, Pietro, Pinoli80, Francesco, Raimondi81, Filippo, Biscarini82, Alessandra, Stella82, Marco, Rizzi83, Franco, Maggiolo83, Diego, Ripamonti83, Claudia, Suardi84, Tiziana, Bachetti85, Maria Teresa La Rovere86, Simona, Sarzi-Braga87, Maurizio, Bussotti88, Katia, Capitani2, Kristina, Zguro2, Simona, Dei90, Sabrina, Ravaglia91, Rosangela, Artuso92, Antonio, Perrella93, Francesco, Bianchi2, Giuseppe, Merla53, Gabriella Maria Squeo94, Mario, Tumbarello2, Ilaria, Rancan2, Davide, Romani30, Manola, Pisani31, Stefano, Busani38, Andrea, Tommasi43, Francesco, Castelli49, Eugenia, Quiros-Roldan49, Alessandra, Guarnaccia61, Oreste De Vivo63, Gabriella, Doddato1, 2, Annarita, Giliberti1, Francesca, Ariani1, Gianluca, Lacerenza95, Elena, Andreucci92, Giulia, Gori92, Angelica, Pagliazzi92, Erika, Fiorentini92, Paola, Bergomi96, Emanuele, Catena96, Riccardo, Colombo96, Sauro, Luchi97, Giovanna, Morelli97, Paola, Petrocelli97, Sarah, Iacopini97, Sara, Modica97, Silvia, Baroni98, Francesco Vladimiro Segala99, Francesco, Menichetti100, Marco, Falcone100, Giusy, Tiseo100, Chiara, Barbieri100, Tommaso, Matucci100, Grassi, Davide, Ferri, Claudio, Marinangeli, Franco, Brancati, Francesco, Antonella, Vincenti104, Valentina, Borgo104, Lombardi, Stefania104, Mirco, Lenzi104, Massimo Antonio Di Pietro105, Francesca, Vichi105, Benedetta, Romanin105, Letizia, Attala105, Cecilia, Costa105, Andrea, Gabbuti105, Menè, Roberto106, Umberto, Zuccon107, Lucia, Vietri107, Patrizia, Casprini108, Marcello Maffezzoni109 and Marta Colaneri110, Croci, Susanna, Venneri, Mary Anna, Mantovani, Stefania, Fallerini, Chiara, Benetti, Elisa, Picchiotti, Nicola, Campolo, Federica, Imperatore, Francesco, Palmieri, Maria, Daga, Sergio, Gabbi, Chiara, Montagnani, Francesca, Beligni, Giada, Farias, Ticiana D J, Carriero, Miriam Lucia, Di Sarno, Laura, Alaverdian, Diana, Aslaksen, Sigrid, Cubellis, Maria Vittoria, Spiga, Ottavia, Baldassarri, Margherita, Fava, Francesca, Norman, Paul J, Frullanti, Elisa, Isidori, Andrea M, Amoroso, Antonio, Mari, Francesca, Furini, Simone, Mondelli, Mario U, Gen-Covid Multicenter Study, Null, Chiariello, Mario, Renieri, Alessandra, and Meloni, Ilaria

Subjects
Male, L412F, SARS-CoV-2, COVID-19, Cell Biology, Biomarker, Polymorphism, Single Nucleotide, Severity of Illness Index, Toll-Like Receptor 3, HLA, Autophagy, TLR3, HEK293 Cell, Molecular Biology, Human, Hydroxychloroquine
Abstract

The polymorphism L412F in TLR3 has been associated with several infectious diseases. However, the mechanism underlying this association is still unexplored. Here, we show that the L412F polymorphism in TLR3 is a marker of severity in COVID-19. This association increases in the sub-cohort of males. Impaired macroautophagy/autophagy and reduced TNF/TNF�� production was demonstrated in HEK293 cells transfected with TLR3L412F-encoding plasmid and stimulated with specific agonist poly(I:C). A statistically significant reduced survival at 28 days was shown in L412F COVID-19 patients treated with the autophagy-inhibitor hydroxychloroquine (p = 0.038). An increased frequency of autoimmune disorders such as co-morbidity was found in L412F COVID-19 males with specific class II HLA haplotypes prone to autoantigen presentation. Our analyses indicate that L412F polymorphism makes males at risk of severe COVID-19 and provides a rationale for reinterpreting clinical trials considering autophagy pathways. Abbreviations: AP: autophagosome; AUC: area under the curve; BafA1: bafilomycin A1; COVID-19: coronavirus disease-2019; HCQ: hydroxychloroquine; RAP: rapamycin; ROC: receiver operating characteristic; SARS-CoV-2: severe acute respiratory syndrome coronavirus 2; TLR: toll like receptor; TNF/TNF-��: tumor necrosis factor

Published
2022

15. Temporary Uterine Artery Embolization Followed by Hysteroscopy to Treat a Case of Acquired Uterine Arteriovenous Malformation (UAVM): Case Report with Systematic Literature Review

Author

M M Ianieri, Campolo F, Cina A, U Catena, Scambia G, I Romito, Iacobelli, and Mastrovito S

Subjects
medicine.medical_specialty, medicine.diagnostic_test, business.industry, medicine.medical_treatment, MEDLINE, Arteriovenous malformation, Cochrane Library, medicine.disease, Curettage, Surgery, Systematic review, Uterine artery embolization, Hysteroscopy, medicine.artery, Medicine, business, Uterine artery
Abstract

Objective: This paper provides information about conservative surgery to correct uterine arteriovenous malformations (UAVMs). The main objectives are to review the current literature on this subject and to share our very interesting and challenging case of UAVM. Our innovative surgical approach consisted in a combined method with temporary uterine artery embolization (UAE) plus hysteroscopic treatment. Materials and Methods: A search of MEDLINE, EMBASE, Web of Sciences, Scopus, ClinicalTrial.gov, OVID and Cochrane Library was performed. Any type of study has been considered including case reports. We identify articles published from the inception to July 2019. Three hundred sixty-three articles were identified: 286 articles were excluded for any reason. Overall, 77 articles were incorporated for further assessment. Results: Four surgical techniques have been employed to treat 125 cases of UAVMs: uterine artery embolization (UAE) (69.9%),dilatation and curettage (D&C) (10.4%), operative hysteroscopy (14.4%), uterine artery ligation (4%). Two patients (1.6%) underwent combined approach. Failed procedures which required a new treatment were twenty-one (16.8%). Forty-five pregnancies after surgical conservative management of UAVMs were reported. Conclusion: UAVM is a rare condition. In literature, there is no gold standard of treatment. For young women who wish to preserve fertility, a conservative surgical management should be recommended. As in our case, using a resorbable agent to perform UAE permitted a normal restoration of the flow in the uterine artery after the hysteroscopic resection.

Published
2020
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16. Targeting the NO-cGMP-PDE5 pathway in COVID-19 infection

Author

Isidori, A M, Giannetta, E, Pofi, R, Venneri, M A, Gianfrilli, D, Campolo, F, Mastroianni, C M, Lenzi, A, and D'Ettorre, G

Subjects
interstitial pneumonia, IL-6, pulmonary fibrosis, type 2 diabetes mellitus, pde5 inhibitors, cytokine storm
Published
2020

17. Indocyanine green in the surgical management of endometriosis: A systematic review

Author

Ianieri, M. M., Della Corte, L., Campolo, F., Cosentino, Francesco, Catena, U., Bifulco, G., Scambia, Giovanni, Cosentino F., Scambia G. (ORCID:0000-0003-2758-1063), Ianieri, M. M., Della Corte, L., Campolo, F., Cosentino, Francesco, Catena, U., Bifulco, G., Scambia, Giovanni, Cosentino F., and Scambia G. (ORCID:0000-0003-2758-1063)

Abstract

Introduction: Endometriosis is a very common disease that affects up to 10% of the female population. The use of indocyanine green (ICG) dye has been proposed to allow the proper localization of endometriotic lesions during surgery. Our purpose is to offer an overview of near-infrared (NIR)-ICG in the surgical treatment of superficial peritoneal endometriosis and deep infiltrating endometriosis. Material and methods: Electronic databases were searched, including MEDLINE, Embase, Web of Science, Scopus, ClinicalTrial.gov, OVID and Cochrane Library. The studies were identified with the use of a mesh combination of the following keywords: “indocyanine green”, “endometriosis”, “deep endometriosis”, “robotic surgery”, “laparoscopy”, “ureter”, “rectosigmoid” from 2000 to May 2020. All articles describing the use of ICG applied to endometriosis surgery were considered for review. Only original papers that reported specific experience data on the topic were included. Moreover, video-articles were included in the analysis. Quality and risk of bias were evaluated by two authors, respectively. Results: Fifty-three studies were reviewed and reviews or comment articles not reporting original data and original articles lacking specific data on the application of ICG in patients affected by endometriosis were excluded. The quality of the 17 studies included was assessed. Eight studies suggested the usefulness of NIR-ICG as a tool in the detection of endometriosis during surgery, and one randomized controlled trial and one prospective study did not confirm the advantage of its use. Eight studies found that NIR-ICG was useful for the evaluation of vascularization in intestinal anastomoses and ureterolysis after surgery for deep infiltrating endometriosis. Conclusions: NIR-ICG appears useful in the evaluation of vascularization in intestinal anastomoses after segmental resection, confirming its role even after ureterolysis for parametrial deep infiltrating endometriosis. However, its

Published
2020

21. European Network of Trainees in Obstetrics and Gynaecology: experiences from the 2016 exchange programme in Turin, Italy

Author

Eismann, S., Brandl, M., Spinnewijn, L., Everden, C., Campolo, F., Schott, S., Eismann, S., Brandl, M., Spinnewijn, L., Everden, C., Campolo, F., and Schott, S.

Abstract

Item does not contain fulltext, INTRODUCTION: The European Network of Trainees in Obstetrics and Gynaecology (ENTOG) is an organization representing trainees from 30 European member countries. Together with the European Board and College of Obstetrics and Gynaecology (EBCOG), it seeks to achieve the highest possible standards of training and consequently to improve the quality of medical care in the field of gynaecology and obstetrics. Every year, the ENTOG council meets and holds a scientific meeting in a different European country. To coincide with this, the host country arranges an exchange, to which each member country can send two trainees. This exchange allows trainees to gain insight into both daily clinical work and the structure of the health care system. METHODS: This article reports the experiences of participants in the May 2016 ENTOG exchange to Turin, Italy. The aim is to outline differences in training between Germany and Italy as well as some striking differences with other European countries. PERSPECTIVE: The participants' personal benefit from this unique experience was not only to get familiar with the Italian trainee programme and health care system, but also to exchange experiences among representatives from other European countries and build up a young gynaecological network within Europe.

Published
2017

22. Qualche domanda (sul) queer in Italia

Author

Pustianaz, M, Lo Iacono, C, Marcasciano, P, Borghi, L, Frabetti, F, Ventrella, F, Bavaro, V, Bernini, L, Campolo, F, Iacoli, G, ANTOSA, Silvia, Pustianaz, M, Lo Iacono, C, Marcasciano, P, Borghi, L, Frabetti, F, Ventrella, F, Bavaro, V, Bernini, L, Campolo, F, Iacoli, G, and Antosa, S

Subjects
sexuality, politics, identity, cultural translation, LGBT, theory, Settore L-LIN/10 - Letteratura Inglese
Published
2010

23. Activated c-Kit receptor in the heart promotes cardiac repair and regeneration after injury

Author

Di Siena, S, primary, Gimmelli, R, additional, Nori, S L, additional, Barbagallo, F, additional, Campolo, F, additional, Dolci, S, additional, Rossi, P, additional, Venneri, M A, additional, Giannetta, E, additional, Gianfrilli, D, additional, Feigenbaum, L, additional, Lenzi, A, additional, Naro, F, additional, Cianflone, E, additional, Mancuso, T, additional, Torella, D, additional, Isidori, A M, additional, and Pellegrini, M, additional

Published
2016
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26. PRINCIPI DI ANATOMIA UMANA capitoli 1, 2, 3, 5

Author

Dolci, C., Donetti, E., Orsini, E., Quaranta, M., Zanetti, D., Campolo, F., Di Giacomo, D., Di Siena, S., Gori, M., and Pellegrini, M.

Subjects
Settore BIO/16 - Anatomia Umana
Published
2012

29. Essential role of sox2 for the establishment and maintenance of the germ cell line

Author

Campolo, F, Gori, M, Favaro, R, Nicolis, S, Pellegrini, M, Botti, F, Rossi, P, Jannini, E, Dolci, S, FAVARO, REBECCA, NICOLIS, SILVIA KIRSTEN, Dolci, S., Campolo, F, Gori, M, Favaro, R, Nicolis, S, Pellegrini, M, Botti, F, Rossi, P, Jannini, E, Dolci, S, FAVARO, REBECCA, NICOLIS, SILVIA KIRSTEN, and Dolci, S.

Abstract

Sox2 is a pluripotency-conferring gene expressed in primordial germ cells (PGCs) and postnatal oocytes, but the role it plays during germ cell development and early embryogenesis is unknown. Since Sox2 ablation causes early embryonic lethality shortly after blastocyst implantation, we generated mice bearing Sox2-conditional deletion in germ cells at different stages of their development through the Cre/loxP recombination system. Embryos lacking Sox2 in PGCs show a dramatic decrease of germ cell numbers at the time of their specification. At later stages, we found that Sox2 is strictly required for PGC proliferation. On the contrary Sox2 deletion in meiotic oocytes does not impair postnatal oogenesis and early embryogenesis, indicating that it is not essential for oocyte maturation nor for zygotic development. We also show that Sox2 regulates Kit expression in PGCs and binds to discrete transcriptional regulatory sequences of this gene, which is known to be important for PGCs survival and proliferation. Sox2 also stimulates the expression of Zfp148 which is required for normal development of fetal germ cells, and Rif1, a potential regulator of PGC pluripotency.

Published
2013

30. SOHLH1 and SOHLH2 control Kit expression during postnatal male germ cell development

Author

Barrios, F, Filipponi, D, Campolo, F, Gori, M, Bramucci, F, Pellegrini, M, Ottolenghi, S, Rossi, P, Jannini, E, Dolci, S, OTTOLENGHI, SERGIO, Dolci, S., Barrios, F, Filipponi, D, Campolo, F, Gori, M, Bramucci, F, Pellegrini, M, Ottolenghi, S, Rossi, P, Jannini, E, Dolci, S, OTTOLENGHI, SERGIO, and Dolci, S.

Abstract

How Kit expression is regulated in the germline remains unknown. SOHLH1 and SOHLH2, two bHLH transcription factors specifically expressed in germ cells, are involved in spermatogonia and oocyte differentiation. In the male, deletion of each factor causes loss of Kitexpressing spermatogonia in the prepuberal testis. In the female, SOHLH1 and SOHLH2 ablations cause oocyte loss in the neonatal ovary. To investigate whether Kit expression is regulated by these two factors in male germ cells, we examined SOHLH1 and SOHLH2 expression during fetal and postnatal mouse development. We found a strong positive correlation between Kit and the two transcription factors only in postnatal spermatogonia. SOHLH2 was enriched in undifferentiated spermatogonia, whereas SOHLH1 expression was maximal at Kit-dependent stages. Expression of SOHLH1, but not SOHLH2, was increased in postnatal mitotic germ cells by treatment with all-trans retinoic acid. We found that E-box sequences within the Kit promoter and its first intron can be transactivated in transfection experiments overexpressing Sohlh1 or Sohlh2. Co-transfection of both factors showed a cooperative effect. EMSA experiments showed that SOHLH1 and SOHLH2 can independently and cooperatively bind an E-box-containing probe. In vivo coimmunoprecipitations indicated that the two proteins interact and overexpression of both factors increases endogenous Kit expression in embryonic stem cells. SOHLH1 was found by ChIP analysis to occupy an E-box-containing region within the Kit promoter in spermatogonia chromatin. Our results suggest that SOHLH1 and SOHLH2 directly stimulate Kit transcription in postnatal spermatogonia, thus activating the signaling involved in spermatogonia differentiation and spermatogenetic progression.

Published
2012

31. Paediatric nephrology

Author

Klein, J., primary, Lacroix, C., additional, Caubet, C., additional, Siwy, J., additional, Muller, F., additional, Bascands, J.-L., additional, Decramer, S., additional, Schanstra, J., additional, Camilla, R., additional, Loiacono, E., additional, Peruzzi, L., additional, Gallo, R., additional, Donadio, M. E., additional, Vergano, L., additional, Campolo, F., additional, Morando, L., additional, Amore, A., additional, Coppo, R., additional, Dossier, C., additional, Leclerc, A.-L., additional, Lapidus, N., additional, Rousseau, A., additional, Charbit, M., additional, Sarda, H., additional, Madhi, F., additional, Carrat, F., additional, Deschenes, G., additional, Harambat, J., additional, Dallocchio, A., additional, Guigonis, V., additional, Ichay, L., additional, Bessenay, L., additional, Broux, F., additional, Garnier, A., additional, Morin, D., additional, Llanas, B., additional, Saint-Marcoux, F., additional, Van Stralen, K., additional, Verrina, E., additional, Belingheri, M., additional, Dusek, J., additional, Dudley, J., additional, Grenda, R., additional, Rubik, J., additional, Rudaitis, S., additional, Rudin, C., additional, Schaefer, F., additional, Jager, K., additional, Loos, S., additional, and Kemper, M. J., additional

Published
2012
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32. Primary and secondary glomerulonephritis I

Author

Miyazaki, N., primary, Matsumoto, J., additional, Alberici, F., additional, Palmisano, A., additional, Maritati, F., additional, Oliva, E., additional, Buzio, C., additional, Vaglio, A., additional, Mjoen, G., additional, Norby, G. E., additional, Vikse, B. E., additional, Svarstad, E., additional, Rune, B., additional, Knut, A., additional, Szymczak, M., additional, Kuzniar, J., additional, Kopec, W., additional, Marchewka, Z., additional, Klinger, M., additional, Arrizabalaga, P., additional, Silvarino, R., additional, Sant, F., additional, Espinosa, G., additional, Sole, M., additional, Cervera, R., additional, Gude, D., additional, Chennamsetty, S., additional, Demin, A., additional, Kozlov, V., additional, Lisukov, I., additional, Kotova, O., additional, Sizikov, A., additional, Sergeevicheva, V., additional, Demina, L., additional, Borjesson, O., additional, Wendt, M., additional, Avik, A., additional, Qureshi, A. R., additional, Bratt, J., additional, Miller, E. J., additional, Gunnarsson, I., additional, Bruchfeld, A., additional, Sugiyama, K., additional, Hasegawa, M., additional, Yamamoto, K., additional, Hayashi, H., additional, Koide, S., additional, Murakami, K., additional, Tomita, M., additional, Yoshida, S., additional, Yuzawa, Y., additional, Yew, S., additional, Jayne, D., additional, Westman, K., additional, Hoglund, P., additional, Flossman, O., additional, Mahr, A., additional, Luqmani, R., additional, Robson, J., additional, Thervet, E., additional, Levi, C., additional, Guiard, E., additional, Roland, M., additional, Nochy, D., additional, Daniliuc, C., additional, Guillevin, L., additional, Mouthon, L., additional, Jacquot, C., additional, Karras, A., additional, Kimura, Y., additional, Morita, H., additional, Debiec, H., additional, Yamada, H., additional, Miura, N., additional, Banno, S., additional, Ronco, P., additional, Imai, H., additional, Shin, D. H., additional, Famee, D., additional, Koo, H. M., additional, Han, S. H., additional, Choi, K. H., additional, Yoo, T.-H., additional, Kang, S.-W., additional, Fofi, C., additional, Scabbia, L., additional, Festuccia, F., additional, Stoppacciaro, A., additional, Mene', P., additional, Shimizu, A., additional, Fukui, M., additional, MII, A., additional, Kaneko, T., additional, Masuda, Y., additional, Iino, Y., additional, Katayama, Y., additional, Fukuda, Y., additional, Kuroki, A., additional, Matsumoto, K., additional, Akizawa, T., additional, Jurubita, R., additional, Ismail, G., additional, Bobeica, R., additional, Rusu, E., additional, Zilisteanu, D., additional, Andronesi, A., additional, Motoi, O., additional, Ditoiu, V., additional, Copaci, I., additional, Voiculescu, M., additional, Irazabal, M. V., additional, Eirin, A., additional, Lieske, J. C., additional, Beck, L. H., additional, Dillon, J. J., additional, Nachman, P. H., additional, Sethi, S., additional, Erickson, S. B., additional, Cattran, D. C., additional, Fervenza, F. C., additional, Svobodova, B., additional, Hruskova, Z., additional, Janatkova, I., additional, Jancova, E., additional, Tesar, V., additional, Seo, M. S., additional, Kwon, S. H., additional, Lee, E. B., additional, You, J. Y., additional, Hyun, Y. K., additional, Woo, S. A., additional, Park, M. Y., additional, Choi, S. J., additional, Jeon, J. S., additional, Noh, H., additional, Kim, J. G., additional, Han, D. C., additional, Hwang, S. D., additional, Choi, T. Y., additional, Jin, S. Y., additional, Loiacono, E., additional, Defedele, D., additional, Puccinelli, M. P., additional, Camilla, R., additional, Gallo, R., additional, Peruzzi, L., additional, Rollino, C., additional, Beltrame, G., additional, Ferro, M., additional, Vergano, L., additional, Campolo, F., additional, Amore, A., additional, Coppo, R., additional, Knoop, T., additional, Bostad, L., additional, Leivestad, T., additional, Bjorneklett, R., additional, Teranishi, J., additional, Yamamoto, R., additional, Nagasawa, Y., additional, Shoji, T., additional, Iwatani, H., additional, Okada, N., additional, Moriyama, T., additional, Yamauchi, A., additional, Tsubakihara, Y., additional, Imai, E., additional, Rakugi, H., additional, Isaka, Y., additional, Doh, F. M., additional, Kim, S. J., additional, Han, D. S., additional, Suzuki, Y., additional, Matsuzaki, K., additional, Suzuki, H., additional, Okazaki, K., additional, Yanagawa, H., additional, Maiguma, M., additional, Muto, M., additional, Sato, T., additional, Horikoshi, S., additional, Novak, J., additional, Hotta, O., additional, Tomino, Y., additional, Gutierrez*, E., additional, Zamora, I., additional, Ballarin, J., additional, Arce, Y., additional, Jimenez, S., additional, Quereda, C., additional, Olea, T., additional, Martinez-Ara, J., additional, Segarra, A., additional, Bernis, C., additional, Garcia, A., additional, Goicoechea, M., additional, Garcia de Vinuesa, S., additional, Rojas, J., additional, Praga, M., additional, Ristovska, V., additional, Petrushevska, G., additional, Grcevska, L., additional, Satake, K., additional, Shimizu, Y., additional, Mugitani, N., additional, Honda, S., additional, Shibuya, K., additional, Shibuya, A., additional, Papale, M., additional, Rocchetti, M. T., additional, DI Paolo, S., additional, Suriano, I. V., additional, D'apollo, A., additional, Vocino, G., additional, Montemurno, E., additional, Varraso, L., additional, Grandaliano, G., additional, Gesualdo, L., additional, Huerta, A., additional, Bomback, A. S., additional, Canetta, P. A., additional, Radhakrishnan, J., additional, Herlitz, L., additional, Stokes, B., additional, D'agati, V., additional, Markowitz, G., additional, Appel, G. B., additional, Mouna, H., additional, Nasr, B. D., additional, Mrabet, I., additional, Ahmed, L., additional, Sabra, A., additional, Mohamed Ammeur, F., additional, Mezri, E., additional, Habib, S., additional, Innocenti, M., additional, Pasquariello, A., additional, Pasquariello, G., additional, Mattei, P., additional, Bottai, A., additional, Fumagalli, G., additional, Bozzoli, L., additional, Samoni, S., additional, Cupisti, A., additional, Caldin, B., additional, Hung, J., additional, Repizo, L., additional, Malheiros, D. M., additional, Barros, R., additional, Woronik, V., additional, Giammarresi, C., additional, Bono, L., additional, Ferrantelli, A., additional, Tortorici, C., additional, Licavoli, G., additional, Rotolo, U., additional, Huang, X., additional, Wang, Q., additional, Shi, M., additional, Chen, W., additional, Liu, Z., additional, Scarpioni, R., additional, Cantarini, L., additional, Lazzaro, A., additional, Ricardi, M., additional, Albertazzi, V., additional, Melfa, L., additional, Concesi, C., additional, Vallisa, D., additional, Cavanna, L., additional, Gungor, G., additional, Ataseven, H., additional, Demir, A., additional, Solak, Y., additional, Biyik, M., additional, Ozturk, B., additional, Polat, I., additional, Kiyici, A., additional, Ozer Cakir, O., additional, Polat, H., additional, Castillo, I., additional, Carreno, V., additional, Aguilar, A., additional, Madero, R., additional, Hernandez, E., additional, Bartolome, J., additional, Gea, F., additional, Selgas, R., additional, El Aggan, H. A. M., additional, El Banawy, H. S., additional, Wagdy, E., additional, Tchebotareva, N., additional, LI, O., additional, Bobkova, I., additional, Kozlovskaya, L., additional, Varshavskiy, V., additional, Golicina, E., additional, Chen, Y., additional, Gong, Z., additional, Chen, X., additional, Tang, L., additional, Zhou, J., additional, Cao, X., additional, Wei, R., additional, Koo, E. H., additional, Park, J. H., additional, Kim, H. K., additional, Kim, M. S., additional, Jang, H. R., additional, Lee, J. E., additional, Huh, W., additional, Kim, D. J., additional, Oh, H. Y., additional, Kim, Y.-G., additional, Eskova, O., additional, Shvetsov, M., additional, Golytsina, E., additional, Popova, O., additional, Quaglia, M., additional, Monti, S., additional, Fenoglio, R., additional, Menegotto, A., additional, Airoldi, A., additional, Izzo, C., additional, Rizzo, M. A., additional, Dianzani, U., additional, Stratta, P., additional, and Gianfreda, D., additional

Published
2012
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33. CKD 5D epidemiology and outcomes

Author

Arnold, R., primary, Pussell, B. A., additional, Grinius, V., additional, Kiernan, M. C., additional, Lin, C. S.-Y., additional, Krishnan, A. V., additional, Defedele, D., additional, Loiacono, E., additional, Puccinelli, M. P., additional, Peruzzi, L., additional, Maffei, S., additional, Camilla, R., additional, Gallo, R., additional, Triolo, G., additional, Bergamo, D., additional, Palazzo, E., additional, Vergano, L., additional, Campolo, F., additional, Amore, A., additional, Coppo, R., additional, Schneider, A., additional, Schneider, M. P., additional, Jardine, A. G., additional, Wanner, C., additional, Drechsler, C., additional, Hecking, M., additional, Karaboyas, A., additional, Rayner, H., additional, Saran, R., additional, Sen, A., additional, Inaba, M., additional, Bommer, J., additional, Horl, W., additional, Pisoni, R., additional, Robinson, B., additional, Sunder-Plassmann, G., additional, Port, F., additional, Usvyat, L. A., additional, Thijssen, S., additional, Kotanko, P., additional, Levin, N. W., additional, Castledine, C., additional, Gilg, J., additional, Rogers, C., additional, Ben-Shlomo, Y., additional, and Caskey, F., additional

Published
2012
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34. TYRGEONET: campagna GPS 1992

Author

Achilli, Vladimiro, Anzidei, M., Baldi, P., Artese, G., Banni, A., Belardinelli, M. E., Bonini, C., Briole, P., Campolo, F., Capone, G., Capra, A., Casula, G., Crespi, M., DI FILIPPO, S., Giovani, L., Guerra, I., Marchesini, C., Marchetti, M., Marsan, P., Mattone, M., Marsella, M., Moretti, A., Poga, K., Poggi, M., Riguzzi, F., Salemi, G., Stoppini, A., Surace, L., Tertulliani, A., Vecchi, M., Vespe, F., and Vettore, Antonio

Published
1993

38. La rete GPS della Calabria

Author

Achilli, Vladimiro, Anzidei, M., Artese, G., Baratin, L., Bonini, C., Campolo, F., Capone, G., Gilormo, A., Guerra, I., Marsan, P., Poggi, M., Strollo, R. M., and Vespe, F.

Published
1992

39. Establishment of a GPS Network in the Tyhrrenian Basin

Author

Achilli, Vladimiro, Anzidei, M., Bacchetti, M., Baldi, P., Banni, A., Barrile, V., Beinat, A., Benciolini, B., Betti, B., Bitelli, G., Briole, P., Campolo, F., Capone, G., Capra, A., Chadi, M., Crespi, M., Di Filippo, S., Favre, F., Giacobbe, G., Grassi, V., Marsan, P., Marsella, M., Nunnari, G., Puglisi, G., Riguzzi, F., Rondoni, C., Salemi, Giuseppe, Souriau, A., Trabelsi, H., Unguendoli, M., Velardita, R., Vettore, Antonio, and Vittuari, L.

Subjects
Network adjustment, GPS
Published
1992

40. La rete GPS della Calabria

Author

Strollo, Rm, Achilli, V, Anzidei, M, Artese, G, Baratin, L, Bonini, C, Campolo, F, Capone, G, Gilormo, S, Guerra, I, Marsan, P, Moretti, A, Poggi, M, Pondrelli, S, and Vespe, F

Subjects
Settore ICAR/17 - Disegno
Published
1992

41. Tyrgeonet: descrizione della rete GPS del Tirreno

Author

Achilli, V., Anzidei, M., Baldi, Paolo, Banni, A., Alberto BEINAT, Benciolini, B., Betti, B., Bitelli, G., Briole, P., Broccio, F., Campolo, F., Capone, G., Capra, A., Chadi, M., Crespi, M., Marco, R., Di Filippo, S., Favre, F., Gasparini, C., Giacobbe, G., Grassi, V., Marchetti, M., Marsan, P., Marsella, M., Nunnari, G., Puglisi, G., Quareni, F., Riguzzi, F., Rondoni, C., Salemi, Giuseppe, Souriau, A., Trabelsi, H., Unguendoli, M., Vecchi, M., Velardita, R., Vettore, A., and Vittuari, L.

Subjects
Rete GPS, Deformazioni crostali, Campo di velocità
Published
1991

44. To Be or Not to Be a Germ Cell: The Extragonadal Germ Cell Tumor Paradigm

Author

Marco Barchi, Susanna Dolci, Carmela Rita Balistreri, Francesca Gioia Klinger, Federica Campolo, Massimo De Felici, De Felici M., Klinger F.G., Campolo F., Balistreri C.R., Barchi M., and Dolci S.

Subjects
Epigenomics, Male, Pluripotent Stem Cells, endocrine system, Cell type, Transcription, Genetic, QH301-705.5, Population, Review, Biology, germline, Catalysis, Germline, Inorganic Chemistry, Testicular Neoplasms, medicine, primordial germ cells, Humans, Epigenetics, Biology (General), Physical and Theoretical Chemistry, education, Gonads, QD1-999, Molecular Biology, Spectroscopy, education.field_of_study, Settore BIO/16, Organic Chemistry, EG cells, Teratoma, Embryo, germ cell tumor, Cell Differentiation, General Medicine, Neoplasms, Germ Cell and Embryonal, medicine.disease, Computer Science Applications, Cell biology, Chemistry, medicine.anatomical_structure, Germ Cells, Extragonadal Germ Cell Tumor, Germ cell tumors, Germ cell
Abstract

In the human embryo, the genetic program that orchestrates germ cell specification involves the activation of epigenetic and transcriptional mechanisms that make the germline a unique cell population continuously poised between germness and pluripotency. Germ cell tumors, neoplasias originating from fetal or neonatal germ cells, maintain such dichotomy and can adopt either pluripotent features (embryonal carcinomas) or germness features (seminomas) with a wide range of phenotypes in between these histotypes. Here, we review the basic concepts of cell specification, migration and gonadal colonization of human primordial germ cells (hPGCs) highlighting the analogies of transcriptional/epigenetic programs between these two cell types.

Published
2021

45. Ketogenic diet: a tool for the management of neuroendocrine neoplasms?

Author

Antongiulio Faggiano, Concetta Sciammarella, Filomena Bottiglieri, Annamaria Colao, Giovanna Muscogiuri, Federica Campolo, Emilia Sbardella, Luigi Barrea, Maria Grazia Tarsitano, Muscogiuri, G., Barrea, L., Campolo, F., Sbardella, E., Sciammarella, C., Tarsitano, M. G., Bottiglieri, F., Colao, A., and Faggiano, A.

Subjects
Oncology, obesity, medicine.medical_specialty, medicine.medical_treatment, Nutritional Status, Diet, High-Fat, Industrial and Manufacturing Engineering, Neoplasms, Internal medicine, medicine, Humans, Cancer, Heterogeneous group, neuroendocrine neoplasm, neuroendocrine neoplasms, business.industry, Incidence (epidemiology), Malnutrition, General Medicine, medicine.disease, Obesity, Nutritional Statu, Neuroendocrine Tumors, stomatognathic diseases, nutrition, ketogenic diet, cancer, Neoplasm, Diet, Ketogenic, business, Human, Food Science, Ketogenic diet
Abstract

Neuroendocrine neoplasms (NENs) are a heterogeneous group of neoplasms, whose incidence has rapidly increased in the last years. Nutrition plays an important role in their management; indeed, malnutrition negatively impacts on rates of complications, hospitalization, hospital stay, costs and mortality. Furthermore, it has been reported that a poor nutritional status could influence the outcome of patients with pancreatic NENs. Moreover, obesity, predisposing to insulin resistance and compensatory hyperinsulinemia, could stimulate the growth of these neoplasms. Ketogenic diet (KD), a high-fat, low-carbohydrate diet with adequate amounts of protein, has been reported to be a promising approach for the management of several types of cancer, mostly gynecological and neurological ones. Indeed, it appears to sensitize most cancers to standard treatment by exploiting the reprogramed metabolism of cancer cells and thus resulting in a promising candidate as an adjuvant cancer therapy. Thus, the aim of this review is to provide an overview on the importance of nutrition in cancer management and in particular in NENs' setting. Furthermore, we reported the current evidence on the efficacy of KD in the management of cancer and based on molecular mechanisms; we also hypothesize the potential use of this nutritional pattern in the management of NENs.

Published
2020

46. Type 5 phosphodiesterase (PDE5) and the vascular tree: from embryogenesis to aging and disease

Author

Eugenia Guida, Susanna Dolci, Federica Campolo, Giovanni Ruvolo, Emmanuele A. Jannini, Carmela Rita Balistreri, Clara Crescioli, Angela Di Baldassarre, Calogera Pisano, Valeriana Cesarini, Cesarini V., Guida E., Campolo F., Crescioli C., Di Baldassarre A., Pisano C., Balistreri C.R., Ruvolo G., Jannini E.A., and Dolci S.

Subjects
0301 basic medicine, Cell type, Aging, Vascular smooth muscle, Myocytes, Smooth Muscle, VSMCs, Embryonic Development, ECs, Context (language use), Disease, Biology, Muscle, Smooth, Vascular, Article, 03 medical and health sciences, 0302 clinical medicine, nitric oxide, vascular smooth muscle cells, Humans, Bioresorbable vascular scaffold, Cyclic Nucleotide Phosphodiesterases, Type 5, EC, Embryogenesis, Phosphodiesterase, Vascular aging, Cell biology, cGMP, Settore MED/23, PDE5, 030104 developmental biology, phosphodiesterase, 030217 neurology & neurosurgery, Function (biology), Developmental Biology
Abstract

Highlights • Vascular development depends on the timely differentiation of endothelial and smooth muscle cells, that mutually influence their developmental fate. • Endothelial and vascular smooth muscle cell (VSMC) compartments can mutually influence cell and tissue modifications during vascular aging and in vascular disease. • Keeping in mind that PDE5 is mainly expressed in VSMCs, we surveyed the literature on the role of PDE5 in vascular development, aging and disease. • Although most results have been obtained by PDE5 pharmacological inhibition, no data are available, to date, on vascular development, aging or disease following PDE5 genetic ablation., Vascular tree development depends on the timely differentiation of endothelial and vascular smooth muscle cells. These latter are key players in the formation of the vascular scaffold that offers resistance to the blood flow. This review aims at providing an overview on the role of PDE5, the cGMP-specific phosphodiesterase that historically attracted much attention for its involvement in male impotence, in the regulation of vascular smooth muscle cell function. The overall goal is to underscore the importance of PDE5 expression and activity in this cell type in the context of the organs where its function has been extensively studied.

Published
2020
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47. Indocyanine green in the surgical management of endometriosis: A systematic review

Author

Giovanni Scambia, Ursula Catena, Francesco Cosentino, Giuseppe Bifulco, Manuel M. Ianieri, Federica Campolo, Luigi Della Corte, Ianieri, M. M., Della Corte, L., Campolo, F., Cosentino, F., Catena, U., Bifulco, G., and Scambia, G.

Subjects
medicine.medical_specialty, indocyanine green, Endometriosis, Ureterolysis, Cochrane Library, Peritoneal Diseases, near-infrared, chemistry.chemical_compound, Robotic Surgical Procedures, medicine, Humans, Ureteral Diseases, deep infiltrating endometriosi, Robotic surgery, Coloring Agents, Laparoscopy, Prospective cohort study, Spectroscopy, Near-Infrared, medicine.diagnostic_test, business.industry, Optical Imaging, Obstetrics and Gynecology, General Medicine, ureterolysis, medicine.disease, deep infiltrating endometriosis, Intestinal Diseases, Settore MED/40 - GINECOLOGIA E OSTETRICIA, Surgery, Computer-Assisted, chemistry, Female, bowel endometriosis, Radiology, Segmental resection, bowel endometriosi, business, Indocyanine green
Abstract

Introduction Endometriosis is a very common disease that affects up to 10% of the female population. The use of indocyanine green (ICG) dye has been proposed to allow the proper localization of endometriotic lesions during surgery. Our purpose is to offer an overview of near-infrared (NIR)-ICG in the surgical treatment of superficial peritoneal endometriosis and deep infiltrating endometriosis. Material and methods Electronic databases were searched, including MEDLINE, Embase, Web of Science, Scopus, ClinicalTrial.gov, OVID and Cochrane Library. The studies were identified with the use of a mesh combination of the following keywords: "indocyanine green", "endometriosis", "deep endometriosis", "robotic surgery", "laparoscopy", "ureter", "rectosigmoid" from 2000 to May 2020. All articles describing the use of ICG applied to endometriosis surgery were considered for review. Only original papers that reported specific experience data on the topic were included. Moreover, video-articles were included in the analysis. Quality and risk of bias were evaluated by two authors, respectively. Results Fifty-three studies were reviewed and reviews or comment articles not reporting original data and original articles lacking specific data on the application of ICG in patients affected by endometriosis were excluded. The quality of the 17 studies included was assessed. Eight studies suggested the usefulness of NIR-ICG as a tool in the detection of endometriosis during surgery, and one randomized controlled trial and one prospective study did not confirm the advantage of its use. Eight studies found that NIR-ICG was useful for the evaluation of vascularization in intestinal anastomoses and ureterolysis after surgery for deep infiltrating endometriosis. Conclusions NIR-ICG appears useful in the evaluation of vascularization in intestinal anastomoses after segmental resection, confirming its role even after ureterolysis for parametrial deep infiltrating endometriosis. However, its usefulness as a tool in the detection of endometriosis during surgery is inconsistent.

Published
2020

48. Essential Role of Sox2 for the Establishment and Maintenance of the Germ Cell Line

Author

Pellegrino Rossi, Rebecca Favaro, Federica Campolo, Flavia Botti, Manuela Pellegrini, Manuele Gori, Emmanuele A. Jannini, Susanna Dolci, Silvia K. Nicolis, Campolo, F, Gori, M, Favaro, R, Nicolis, S, Pellegrini, M, Botti, F, Rossi, P, Jannini, E, and Dolci, S

Subjects
Male, Sox2, Gene Expression, Transgenic, Mice, Primordial germ cells, Specification, Animals, Cell Differentiation, Cells, Cultured, DNA-Binding Proteins, Female, Germ Cells, Mice, Transgenic, Oocytes, Pluripotent Stem Cells, SOXB1 Transcription Factors, Signal Transduction, Telomere-Binding Proteins, Transcription Factors, Settore BIO/16, Cultured, Embryo, Cell biology, medicine.anatomical_structure, Kit tyrosine kinase, embryogenesis, germ cell line, transcription, embryonic structures, Molecular Medicine, Germ line development, Stem cell, Germ cell, endocrine system, Cells, Biology, Sox2, germ cells, stem cells, conditional knockout, SOX2, medicine, Germ plasm, urogenital system, fungi, Cell Biology, Oocyte, Embryonic stem cell, Molecular biology, Developmental Biology
Abstract

Sox2 is a pluripotency-conferring gene expressed in primordial germ cells (PGCs) and postnatal oocytes, but the role it plays during germ cell development and early embryogenesis is unknown. Since Sox2 ablation causes early embryonic lethality shortly after blastocyst implantation, we generated mice bearing Sox2-conditional deletion in germ cells at different stages of their development through the Cre/loxP recombination system. Embryos lacking Sox2 in PGCs show a dramatic decrease of germ cell numbers at the time of their specification. At later stages, we found that Sox2 is strictly required for PGC proliferation. On the contrary, Sox2 deletion in meiotic oocytes does not impair postnatal oogenesis and early embryogenesis, indicating that it is not essential for oocyte maturation or for zygotic development. We also show that Sox2 regulates Kit expression in PGCs and binds to discrete transcriptional regulatory sequences of this gene, which is known to be important for PGCs survival and proliferation. Sox2 also stimulates the expression of Zfp148, which is required for normal development of fetal germ cells, and Rif1, a potential regulator of PGC pluripotency.

Published
2013
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49. SOHLH1 and SOHLH2 control kit expression during postnatal male germ cell development

Author

Emmanuele A. Jannini, Florencia Barrios, Susanna Dolci, Sergio Ottolenghi, Federica Bramucci, Federica Campolo, Manuela Pellegrini, Manuele Gori, Pellegrino Rossi, Doria Filipponi, Barrios, F, Filipponi, D, Campolo, F, Gori, M, Bramucci, F, Pellegrini, M, Ottolenghi, S, Rossi, P, Jannini, E, and Dolci, S

Subjects
endocrine system, germ cells, ggerm cells, kit, sohlh1, sohlh2, animals, basic helix-loop-helix transcription factors, female, gene expression regulation, developmental, HEK293 cells, humans, male, mice, inbred strains, transgenic, proto-oncogene proteins c-kit, spermatogonia, Retinoic acid, Mice, Inbred Strains, Mice, Transgenic, Endogeny, c-kit gene, Biology, Germline, Settore MED/13 - Endocrinologia, chemistry.chemical_compound, transcription factors, medicine, Molecular Biology, Transcription factor, Regulation of gene expression, Settore BIO/16, Gene Expression Regulation, Developmental, Cell Biology, Oocyte, Molecular biology, Embryonic stem cell, Cell biology, medicine.anatomical_structure, chemistry, Proto-Oncogene Proteins c-kit, Oocyte differentiation, Germ cell, Developmental Biology
Abstract

How Kit expression is regulated in the germline is still an open question. SOHLH1 and SOHLH2, two bHLH transcription factors specifically expressed in germ cells, have been recently involved in the differentiation of spermatogonia and oocytes. In the male, deletion of each transcription factor leads to the disappearance of Kit-expressing spermatogonia in the prepuberal testis. In the female, both SOHLH1 and SOHLH2 ablations are responsible of oocyte loss in the neonatal ovary. To investigate if Kit expression might be regulated by these two transcription factors in male germ cells, we have first performed an expression study of SOHLH1 and SOHLH2 during fetal and postnatal development. In these experiments we found a strong positive correlation between Kit and the two transcription factors only in postnatal spermatogonia. SOHLH2 was found enriched in undifferentiated spermatogonia, whereas SOHLH1 expression was maximal in Kit-dependent stages. Expression of SOHLH1, but not SOHLH2, was increased in postnatal mitotic germ cells by treatment with All-trans Retinoic Acid (AtRA), an analog of retinoic acid. We also found that E-box sequences contained within the Kit promoter and its first intron can be transactivated in transfection experiments overexpressing either Sohlh1or Sohlh2. Co-transfection of both factors showed a cooperative effect. EMSA experiments further showed that SOHLH1 and SOHLH2 can independently and cooperatively bind an E-box containing probe. In vivo, co-immunoprecipitation results evidenced that the two proteins interact and overexpression of both factors increases endogenous Kit expression in ES cells. Finally, by ChIP analysis, SOHLH1 was found to occupy an E-box containing region within kit promoter in spermatogonia chromatin. Altogether, our results suggest that SOHLH1 and SOHLH2 directly stimulate Kit transcription in postnatal spermatogonia, thus activating the signalling involved in spermatogonia differentiation and spermatogenetic progression.

Published
2012

50. Anatomical-based classification of dorsolateral parametrectomy for deep endometriosis. Correlation with surgical complications and functional outcomes: A single- center prospective study.

Author

Ianieri MM, Alesi MV, Querleu D, Ercoli A, Chiantera V, Carcagnì A, Campolo F, Greco P, and Scambia G

Subjects
Humans, Female, Prospective Studies, Adult, Treatment Outcome, Gynecologic Surgical Procedures methods, Gynecologic Surgical Procedures adverse effects, Endometriosis surgery, Postoperative Complications epidemiology
Abstract

Objective: To evaluate complication rate and functional outcomes of nerve-sparing parametrectomy for deep endometriosis in relation to the extension of the surgical procedure, based on recognizable anatomical landmarks., Methods: This was a prospective single-center study including all patients undergoing parametrectomy for deep endometriosis from September 2020 to June 2023 at our tertiary center. Dorsolateral parametrectomies were divided into parametrectomies medial to the presacral fascia and cranial to the medial rectal artery (superficial parametrectomy), and parametrectomies in which one of the two landmarks was overcome during the surgical procedure, leading to the excision of tissue lateral to the presacral fascia (deep parametrectomy type 1, or DP1) or caudal to the medial rectal artery (DP2). Finally, we used the hypogastric fascia as landmark to define type 3 deep parametrectomy (DP3), when the procedure was deeply lateral to the fascia., Results: Bladder voiding deficit occurred in 9.7% of cases, with higher rates in DP2 (20.8%) and DP3 (30%) groups. Regarding postoperative gastrointestinal function, our data showed a significant improvement over time in all groups, with the exception of DP2; instead an improvement in postoperative bladder function was only shown in DP3. Parametrectomy was not associated with a simultaneous improvement in sexual function expressed with the female sexual function index, in any of the four groups., Conclusion: Our classification constitutes a concrete approach for comparing, in a standardized way, the complications and functional outcomes of parametrectomy, which, even if carried out by expert surgeons, demonstrates a non-negligible rate of bladder voiding deficit., (© 2024 The Author(s). International Journal of Gynecology & Obstetrics published by John Wiley & Sons Ltd on behalf of International Federation of Gynecology and Obstetrics.)

Published
2024
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