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Turfs are among the major benthic components of reef systems worldwide. The nearly complete genome sequences, basic physiological characteristics, and phylogenomic reconstruction of two phycobiliprotein-rich filamentous cyanobacteria strains isolated from turf assemblages from the Abrolhos Bank (Brazil) are investigated. Both Adonisia turfae CCMR0081T (= CBAS 745T) and CCMR0082 contain approximately 8 Mbp in genome size and experiments identified that both strains exhibit chromatic acclimation. Whereas CCMR0081T exhibits chromatic acclimation type 3 (CA3) regulating both phycocyanin (PC) and phycoerythrin (PE), CCMR0082 strain exhibits chromatic acclimation type 2 (CA2), in correspondence with genes encoding specific photosensors and regulators for PC and PE. Furthermore, a high number and diversity of secondary metabolite synthesis gene clusters were identified in both genomes, and they were able to grow at high temperatures (28 °C, with scant growth at 30 °C). These characteristics provide insights into their widespread distribution in reef systems.

Made available in DSpace on 2018-12-11T17:21:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-07-09 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Marine biotechnology is an emerging field in Brazil and includes the exploration of marine microbial products, aquaculture, omics, isolation of biologically active compounds, identification of biosynthetic gene clusters from symbiotic microorganisms, investigation of invertebrate diseases caused by potentially pathogenic marine microbes, and development of antifouling compounds. Furthermore, the field also encompasses description of new biological niches, current threats, preservation strategies as well as its biotechnological potential. Finally, it is important to depict some of the major approaches and tools being employed to such end. To address the challenges of marine biotechnology, the Brazilian government, through the Ministry of Science, Technology, Innovation, and Communication, has established the National Research Network in Marine Biotechnology (BiotecMar) (www.biotecmar.sage.coppe.ufrj.br). Its main objective is to harness marine biodiversity and develop the marine bioeconomy through innovative research. Institute of Biology and COPPE Federal University of Rio de Janeiro Institute of Biology University of Brasilia Institute de Química de São Carlos Universidade de São Paulo Institute for Sea Studies Admiral Paulo Moreira Institute of Biological and Health Sciences Federal University of Alagoas Institute of Medical Biochemistry Universidade Federal do Rio de Janeiro Faculty of Pharmacy and Biotechnology Center Federal University of Rio Grande do Sul Phytotechny Department Federal University of Santa Catarina Academic Center of Vitória Federal University of Pernambuco Institute of Biology Federal Fluminense University Institute for Marine Sciences Federal University of Ceará Department of Biological Sciences State University of Santa Cruz Aquaculture Center São Paulo State University Institute of Oceanography Federal University of Rio Grande Federal University of Paraiba UFPB Federal University of Pará UFPA University of São Paulo USP Northern Rio State University UENF Federal University of Santa Catarina UFSC State University of Santa Cruz UESC Federal University of Alagoas UFAL Universidade Federal Rural de Pernambuco UFRPE Federal University of Pernambuco UFPE LNCC National Laboratory For Scientific Computing LNCC Oswaldo Cruz Foundation/Rondônia FIOCRUZ Federal University of Bahia UFBA UFRJ Oswaldo Cruz Institute IOC-FIOCRUZ IOC-FIOCRUZ Federal University of São Paulo UNIFESP Ministry of Health MS MCTIC State University of West Rio de Janeiro UEZO National Instute of Industrial Property INPI UnB UNESP Aquaculture Center São Paulo State University UNESP FAPESP: N° 408968/2013-3 CNPq

Made available in DSpace on 2015-03-04T18:58:03Z (GMT). No. of bitstreams: 1 thesisMarianaCampeao.pdf: 13162328 bytes, checksum: ed5b7096328fd1404655a6f926f0f292 (MD5) Previous issue date: 2014-04-25 Vibrios sao bacterias amplamente distribuidas no meio aquatico e podem ser encontradas em associacao com organismos marinhos, tanto como causadores de doencas quanto como simbiontes. O advento das tecnicas de sequenciamento de nova geracao e de alto desempenho tem possibilitado o acesso cada vez mais amplo a dados genomicos microbianos, incluindo vibrios. Tal quantidade e disponibilidade de dados permitem analises in silico, que podem compreender desde caracteristicas genomicas ate fenotipicas. A taxonomia microbiana e fundamentada na abordagem polifasica, que mede as relacoes evolutivas a partir do uso de sequencias de genes, especialmente o RNAr 16S, similaridade genomica, por meio de hibridizacao de DNA, e ampla caracterizacao fenotipica. A caracterizacao fenotipica requer testes experimentais, que muitas vezes sao demorados, caros e requerem grande experiencia. Neste estudo propomos o uso de genomas para a analise da diversidade e identificacao fenotipica de vibrios. Para tanto, foram avaliadas caracteristicas basicas de vibrios (tais como tamanho do genoma, conteudo genico e posicao logenetica); analisaram-se genes unicos e suas possiveis funcoes ecologicas; e desenvolveu-se uma ferramenta prototipo para identificacao de fenotipos diagnosticos de vibrios, denominada vibriophenotyping 1.0. A logenia construida a partir do genoma minimo recuperou os diferentes generos e clados descritos na literatura para o grupo vibrio, bem como posicionou as especies consideradas irmas em relacao a um ancestral comum proximo. Os genes unicos, por sua vez, puderam ainda revelar peculiaridades entre especies irmas. Por m, o programa de identificacao fenotipica desenvolvido foi testado com genomas de linhagens tipo de vibrios e apresentou uma media de similaridade superior a 70% entre os fenotipos obtidos in vitro e in silico, sendo alcançada uma similaridade de 100% para genomas integros. Dessa forma, concluiu-se que analises do pangenoma permitem a recontrucao logenetica dentro do grupo vibrios e a identificacao de genes unicos relevantes para o papel ecologico da especie no ambiente de origem, e, ainda, que a identificacao fenotipica atraves da automatizacao por uma ferramenta computacional e possivel a partir da analise de genomas.