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The objective of this study was to assess carcass traits' influence on pork eating quality as evaluated by consumers. A total of 1360 pork chops were used, with 824 from the sirloin end and 536 from the butt end of the loin (Longissimuss thoracis et lumborum), to produce 340 packages, each containing four pork chops. Untrained participants received one package of either sirloin or butt chops, being two pork chops from barrows and two from gilts. Participants answered a survey rating the tenderness, juiciness, flavour, and overall acceptability of each chop on an 8-point scale. Correlation analysis was conducted between carcass traits and pork eating quality attributes. For the descriptive analysis, classes (low, medium, and high) for carcass traits, Warner-Bratzler shear force (WBSF) and cooking loss were created based on our consumer responses dataset for palatability attributes. No significant correlations (P > 0.05) were observed between carcass traits and pork eating quality traits. Tenderness and overall acceptability were negatively correlated (P < 0.05) with cooking loss and WBSF. Loin intramuscular fat (IMF) content showed a weak negative correlation (P < 0.05) with WBSF and cooking loss. Consumers rated chops from the high and medium/high backfat thickness and loin IMF classes slightly higher for tenderness and juiciness, respectively. Additionally, chops from the low and/or medium WBSF and cooking loss classes received slightly higher scores for tenderness and juiciness than pork chops in the high classes. In conclusion, the study indicated that carcass traits had minimal impact on overall acceptability of pork by consumers., Competing Interests: Declaration of Competing Interest The authors have no competing of interest to declare., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

From a population of 351 pork carcasses, 3.0-cm thick chops from the 10th rib location of the longissimus thoracis, faced surfaces of the triceps brachii and serratus ventralis muscles from the boneless shoulder, and faced surfaces of the biceps femoris, semitendinosus, semimembranosus, adductor, rectus femoris, and vastus lateralis muscles from the boneless ham were evaluated for pH and instrumental color (Minolta CR-400). Evaluations were conducted for at least three different locations on each of the muscle samples and averaged before data analysis occurred. The longissimus thoracis had the lowest pH and the lightest and least red color compared with the other eight muscles evaluated in this study (mean differences ranged from 0.98 to 8.70 for L*, 3.98 to 12.56 for a*, and 0.026 to 0.409 for pH). Furthermore, regression analysis suggested that pH and color values for the longissimus thoracis were not adequate predictors for pH and color values obtained from other muscles and therefore consideration should be given to the individual muscles that are of interest., Competing Interests: Declaration of Competing Interest The authors declare no conflicts of interest., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Objectives of this research were to compare carcass characteristics, carcass cutting yields, and meat quality for market barrows and market gilts. Commercially-sourced carcasses from 168 market barrows and 175 market gilts weighing an average of 107.44 ± 7.37 kg were selected from 17 different slaughter groups representing approximately 3,950 carcasses. Each group was sorted into percentiles based on hot carcass weight with an equal number of barrows and gilts selected from each quartile so that weight minimally confounded parameters of interest. Carcass lean yield was determined for carcasses following fabrication (i.e. dissection of lean, fat, and bone tissue components) and meat quality measurements were evaluated at the time of fabrication (24 to 72 h postmortem) and following 14-d of postmortem storage. Data were analyzed as a randomized complete block design with carcass serving as the experimental unit, sex (barrow or gilt), the three hot carcass weight quantiles (light [<104 kg]; average [104 to 110 kg]; heavy [>110 kg]), and the interaction between sex and hot carcass weight quantile serving as fixed effects, and producer nested within slaughter event serving as a random effect. Results from the study demonstrated that gilt carcasses were leaner (3 mm less backfat thickness; 3.5 cm 2 greater loin muscle area, 1.52% greater merchandized-cut yield, and 2.92% greater dissected carcass lean yield; P < 0.01) than barrow carcasses, while loins from barrows were higher quality (0.43% more intramuscular fat and slightly less shear force; P < 0.01) than loins from gilts. While this study confirms the well-known biological principle that barrow carcasses have greater levels of fat deposition and lower levels of carcass leanness when compared with gilt carcasses, this study provides a much-needed quantification of these differences for the commercial industry that will undoubtedly be useful as new technologies emerge in upcoming years., Competing Interests: The authors declare no conflicts of interest., (© The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science.)

This study compared the accuracy of two methods for predicting carcass leanness (i.e., predicted lean yield) with fat-free lean yields obtained by manual carcass side cut-out and dissection of lean, fat, and bone components. The two prediction methods evaluated in this study estimated lean yield by measuring fat thickness and muscle depth at one location with an optical grading probe (Destron PG-100) or by scanning the entire carcass with advanced ultrasound technology (AutoFom III). Pork carcasses (166 barrows and 171 gilts; head-on hot carcass weights (HCWs) ranging from 89.4 to 138.0 kg) were selected based on their fit within desired HCW ranges, their fit within specific backfat thickness ranges, and sex (barrow or gilt). Data (n = 337 carcasses) were analyzed using a 3 × 2 factorial arrangement in a randomized complete block design including the fixed effects of the method for predicting lean yield, sex, and their interaction, and random effects of producer (i.e., farm) and slaughter date. Linear regression analysis was then used to examine the accuracy of the Destron PG-100 and AutoFom III data for measuring backfat thickness, muscle depth, and predicted lean yield when compared with fat-free lean yields obtained with manual carcass side cut-outs and dissections. Partial least squares regression analysis was used to predict the measured traits from image parameters generated by the AutoFom III software. There were method differences (P < 0.01) for determining muscle depth and lean yield with no method differences (P = 0.27) for measuring backfat thickness. Both optical probe and ultrasound technologies strongly predicted backfat thickness (R2 ≥ 0.81) and lean yield (R2 ≥ 0.66), but poorly predicted muscle depth (R2 ≤ 0.33). The AutoFom III improved accuracy [R2 = 0.77, root mean square error (RMSE) = 1.82] for the determination of predicted lean yield vs. the Destron PG-100 (R2 = 0.66, RMSE = 2.22). The AutoFom III was also used to predict bone-in/boneless primal weights, which is not possible with the Destron PG-100. The cross-validated prediction accuracy for the prediction of primal weights ranged from 0.71 to 0.84 for bone-in cuts and 0.59 to 0.82 for boneless cut lean yield. The AutoFom III was moderately (r ≤ 0.67) accurate for the determination of predicted lean yield in the picnic, belly, and ham primal cuts and highly (r ≥ 0.68) accurate for the determination of predicted lean yield in the whole shoulder, butt, and loin primal cuts., (© The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

The objective was to update the equation used for prediction of pork carcass leanness with the Destron PG-100 optical grading probe. A recent cutout study (completed in 2020-2021) consisting of 337 pork carcasses was used for this research. An updated equation was generated using a calibration dataset (N = 188 carcasses) and prediction precision and prediction accuracy of the new equation was evaluated using a validation dataset (N = 149 carcasses). The updated equation was generated using forward stepwise multiple regression selection techniques in PROC REG of SAS, and the same parameters as the existing equation were used to fit the model. The updated Destron equation [89.16298 - (1.63023 × backfat thickness) - (0.42126 × muscle depth) + (0.01930 × backfat thickness2) + (0.00308 × muscle depth2) + (0.00369 × backfat thickness × muscle depth)] and the existing Destron equation [68.1863 - (0.7833 × backfat thickness) + (0.0689 × muscle depth) + (0.0080 × backfat thickness2) - (0.0002 × muscle depth2) + (0.0006 × backfat thickness × muscle depth)] were similar in their prediction precision for determination of carcass lean yield (LY), with the updated equation R2 = 0.75 and root mean square error (RMSE) = 1.97 and the existing equation R2 = 0.75 and RMSE = 1.94. However, when prediction accuracy was evaluated using the variance explained by predictive models based on cross-validation (VEcv) and Legates and McCabe's efficiency coefficient (E1), the updated equation (VEcv = 67.97%; E1 = 42.41%) was much more accurate compared with the existing equation (VEcv = -117.53%; E1 = -69.24%). Furthermore, when accuracy was evaluated by separating carcasses into 3% carcass LY groupings ranging from less than 50% LY to greater than 62% LY, the existing equation correctly estimated carcass LY 8.1% of the time, while the updated equation correctly estimated carcass LY 47.7% of the time. In an effort to further compare the abilities of the updated equation, comparisons were made with an advanced automated ultrasonic scanner (AutoFom III), which scans the entire carcass. The prediction precision of the AutoFom III was R2 = 0.83 and RMSE = 1.61, while the AutoFom III correctly estimated carcass LY 38.2% of the time and prediction accuracy calculations for the AutoFom III were VEcv = 44.37% and E1 = 21.34%). Overall, refinement of the Destron PG-100 predicted LY equation did not change prediction precision, but substantially improved prediction accuracy., (© The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Autotrophic microorganisms catalyze the entry of dissolved inorganic carbon (DIC; = CO2 + HCO3- + CO32-) into the biological component of the global carbon cycle, despite dramatic differences in DIC abundance and composition in their sometimes extreme environments. "Cyanobacteria" are known to have CO2 concentrating mechanisms (CCMs) to facilitate growth under low CO2 conditions. These CCMs consist of carboxysomes, containing enzymes ribulose 1,5-bisphosphate oxygenase and carbonic anhydrase, partnered to DIC transporters. CCMs and their DIC transporters have been studied in a handful of other prokaryotes, but it was not known how common CCMs were beyond "Cyanobacteria". Since it had previously been noted that genes encoding potential transporters were found neighboring carboxysome loci, α-carboxysome loci were gathered from bacterial genomes, and potential transporter genes neighboring these loci are described here. Members of transporter families whose members all transport DIC (CHC, MDT and Sbt) were common in these neighborhoods, as were members of the SulP transporter family, many of which transport DIC. 109 of 115 taxa with carboxysome loci have some form of DIC transporter encoded in their genomes, suggesting that CCMs consisting of carboxysomes and DIC transporters are widespread not only among "Cyanobacteria", but also among members of "Proteobacteria" and "Actinobacteria"., (© FEMS 2020.)

Background and Objectives: Birthweight differences between kwashiorkor and marasmus suggest that intrauterine factors influence the development of these syndromes of malnutrition and may modulate risk of obesity through dietary intake. We tested the hypotheses that the target protein intake in adulthood is associated with birthweight, and that protein leveraging to maintain this target protein intake would influence energy intake (EI) and body weight in adult survivors of malnutrition., Methodology: Sixty-three adult survivors of marasmus and kwashiorkor could freely compose a diet from foods containing 10, 15 and 25 percentage energy from protein (percentage of energy derived from protein (PEP); Phase 1) for 3 days. Participants were then randomized in Phase 2 (5 days) to diets with PEP fixed at 10%, 15% or 25%., Results: Self-selected PEP was similar in both groups. In the groups combined, selected PEP was 14.7, which differed significantly (P < 0.0001) from the null expectation (16.7%) of no selection. Self-selected PEP was inversely related to birthweight, the effect disappearing after adjusting for sex and current body weight. In Phase 2, PEP correlated inversely with EI (P = 0.002) and weight change from Phase 1 to 2 (P = 0.002). Protein intake increased with increasing PEP, but to a lesser extent than energy increased with decreasing PEP., Conclusions and Implications: Macronutrient intakes were not independently related to birthweight or diagnosis. In a free-choice situation (Phase 1), subjects selected a dietary PEP significantly lower than random. Lower PEP diets induce increased energy and decreased protein intake, and are associated with weight gain., (© The Author(s) 2015. Published by Oxford University Press on behalf of the Foundation for Evolution, Medicine, and Public Health.)

The effects of chilling method and moisture enhancement were examined for improving eating quality of semimembranosus (SM) and longissimus lumborum (LL) from 62 cull beef cows. Chilling method included hot boning muscles after 45 to 60 min postmortem or conventional chilling for 24 h. Moisture enhancement included 1) a non-injected control (CONT) or injection processing (10% of product weight) using 2) Sodium Tripolyphosphate/salt (Na/STP), 3) Sodium Citrate (NaCIT), 4) Calcium Ascorbate (CaASC), or 5) Citrus Juices (CITRUS). Chilling method by moisture enhancement treatment interactions (P<0.09) were due to decreased hue, chroma and sarcomere length values in hot boned vs. conventionally chilled product (SM and LL) for CaASC vs. other moisture enhancement treatments. Chilling method by moisture enhancement treatment interactions (P<0.05) were due to decreased shear force and increased tenderness in conventionally chilled vs. hot boned LL using CaASC vs. Na/STP. Moisture enhancement can improve tenderness of cull cow beef depending on combinations of chilling method and moisture enhancement treatments used., (© 2013.)

Sixty-two cull beef cows were slaughtered to investigate effects of skeletal separation and moisture enhancement on beef eating quality. Muscles from each carcass side were randomly assigned to 1) no postmortem processing (NPP), 2) prerigor skeletal separation (SS), 3) moisture enhancement (ME) using calcium ascorbate or 4) a combination of SS and ME (SS/ME). Postmortem processing treatment (PPT) by ageing (PM) interactions (P<0.01) for shear force were present for longissimus. As PM ageing increased from 7 to 21 d, there was a greater decrease (P<0.05) in shear force with NPP vs. all other PPT. Trained taste panellists found SS, ME and SS/ME improved (P<0.05) palatability attributes vs. NPP. An additive effect of combining SS and ME improved palatability traits versus SS or ME alone. Panellists found no differences (P>0.14) in softness and tenderness between SS/ME and Canadian AA or AAA beef. Postmortem processing of beef cows may produce beef as tender and juicy as beef from younger carcasses., (Copyright © 2012 Elsevier Ltd. All rights reserved.)

Background/objectives: We hypothesized that maternal size during pregnancy and birth size are determinants of childhood physical activity energy expenditure (PAEE). Also, childhood PAEE is inversely related to adiposity and levels of cardiovascular risk factors., Subjects/methods: The Vulnerable Windows Cohort Study is a longitudinal observational study of 569 Afro-Jamaican mothers recruited from the first trimester and their offspring. Anthropometry, bioelectrical impedance, PAEE (using the Actical monitor) and cardiovascular risk factors (blood pressure, fasting glucose, insulin and lipids) were measured in 124 boys and 160 girls at a mean age of 13.2 years., Results: Boys had more fat-free mass (FFM) and expended more energy than girls (12.3±3.3 vs 9.6±2.8 kcal/kg/day; P<0.001). Maternal weight was associated with child's PAEE (r=0.29; P<0.001). PAEE was not significantly associated with birth weight. Maternal weight, after adjusting for child's age and sex, was positively associated with the child's FFM, fat mass and %fat (P-values 0.01). Age- and sex-adjusted PAEE was positively associated with FFM, fat mass and % fat (P-values <0.001), but not after adjusting for current weight. Age- and sex-adjusted PAEE was positively associated with triglycerides, insulin and systolic blood pressure (P-values <0.05), but not after adjusting for weight and height. PAEE was associated with fasting glucose after controlling for age, sex, weight and height (r=-0.12; P=0.02)., Conclusions: Maternal size, but not birth weight, is a determinant of childhood PAEE. PAEE is not strongly associated with childhood body composition, but is inversely related to fasting glucose concentration.

The products of the flowering plant, lupin, are increasingly used as a human food product, particularly in baking. Occupational sensitization to lupin with occupational rhinitis, conjunctivitis and asthma was first described in 2001, and confirmed in a larger cross-sectional study in a food processing company in 2006. Sensitization by inhalation may result in occupational asthma, work-exacerbated asthma, occupational rhinitis and conjunctivitis. The incidence of occupational sensitization may be as high as 29%. The relationship with exposure intensity is as yet unclear, and requires further clarification. Although there is little information from long-term studies, these diseases are likely to improve after cessation of exposure. Cross-sensitization to other legumes, particularly peanuts, has been shown by skin prick testing, with potential for serious anaphylactic reactions. This review summarizes the available literature on occupational sensitization to lupin products. It is one of two reviews, one covering the problem of lupin allergy in the home, while the present article deals with lupin sensitization in the workplace. Increased awareness is needed of this occupational hazard to avoid future cases of occupational disease and their accompanying morbidity and potential mortality., (© 2010 Blackwell Publishing Ltd.)

Background: Lupin is a legume. Its seed can be ground into flour and incorporated into food as a protein source. Cases of rhinitis, urticaria, and anaphylaxis from ingestion of lupin have been reported as well as asthma., Objective: To present a cross-sectional study of workers in a food processing company who were exposed to lupin and developed occupational allergy secondary to inhaling lupin products., Methods: Subjects were interviewed using a standardized questionnaire, including demographics and current and past symptoms. They underwent skin prick tests (SPTs) to common aeroallergens and lupin products, spirometry, and off-line exhaled nitric oxide measurement. Symptomatic subjects, sensitized to lupin on SPT, underwent methacholine bronchial provocation challenge. Those with bronchial hyperresponsiveness had specific bronchial provocation challenge to lupin., Results: A total of 53/54 subjects completed testing (98%). Overall, 21% (11/53) had positive SPT results to lupin. The lupin-sensitive group had a trend toward atopy (P = .06). Seven of 11 (64%) subjects in this group were symptomatic; all had rhinitis, and 2 had wheeze. Two subjects had positive methacholine challenges, and 1 had a positive specific bronchial provocation challenge to lupin with both an early-phase and a late-phase response., Conclusion: Allergy to inhaled lupin occurs in the workplace. A high sensitization rate on SPT was found, which correlated with symptoms. The clinical significance of cross-reactivity between legumes on SPT is unclear., Clinical Implications: Sensitization to the legume, lupin, can occur from exposure at work and carries a high prevalence of clinical symptoms, which in some cases leads to occupational rhinitis and asthma.

Limousin-cross steers (n=135; 258+/-26 kg) were used to compare forage vs grain feeding on carcass composition and palatability attributes of beef when time on feed was controlled. Diets included a 95% alfalfa silage ration (AS) or a 68% high-moisture corn, 25% alfalfa silage ration (HMC). These were incorporated into six treatments to allow comparisons of end points based on similar days on feed or backfat finish. Dietary treatments included 1) HMC (4 mm), or cattle allowed ad libitum intake of HMC until slaughter at 4 mm ultrasound backfat; 2) AS (HMC-4), or cattle allowed ad libitum intake of AS until slaughter, regardless of finish, when HMC (4 mm) cattle were slaughtered; 3) AS (4 mm), or cattle allowed ad libitum intake of AS until slaughter at 4 mm backfat; 4) HMC (AS-4), or cattle allowed ad libitum intake of HMC until slaughter, regardless of finish, when AS (4 mm) cattle achieved 4 mm backfat; 5) HMC (RES), or cattle fed HMC at restricted intakes until slaughter at 4 mm backfat with feed offered at 75% of intake achieved by HMC (4 mm) and HMC (AS-4) cattle; and 6) AS (8 mm) or cattle allowed ad libitum intake of AS ration until slaughter at 8 mm backfat. Grain feeding generally increased (P < .01) ADG, carcass weight, grade fat, and intramuscular fat content when compared with forage feeding at similar times on feed. Palatability attributes of ribeye roasts and ground beef were generally unaffected (P > .10) by diet with the exception of slightly less beef flavor and more off-flavor in forage-fed vs grain-fed beef. Higher (P < .01) concentrations of linolenic acid and lower (P < .10) concentrations of oleic acid in forage-fed beef may be partially responsible for diet differences in flavor.

We investigated the effects of fish meal (FM) in beef cattle diets on growth performance, carcass characteristics, and fatty acid (FA) composition of longissimus muscle in 63 yearling steers (335 +/- 23 kg). High-moisture corn and alfalfa silage diets were supplemented with either a corn gluten/blood meal mixture or FM at 10% of the diet. Fish meal contained (as-is basis) 5.87 g/kg eicosapentaenoic acid (EPA) and 9.84 g/kg docosahexaenoic acid (DHA). Seven strategies were developed to feed either a control diet (no FM) or diets containing 5 or 10% FM with FM fed for either 56, 112, or 168 d before slaughter. Average daily gain and feed efficiency were not affected (P > .10) by FM feeding but DMI decreased. Within FM diets, cattle fed 5% FM consumed more (P < .01) DM and gained more (P < .02) than cattle fed 10% FM. Carcass traits were not affected (P > .05) by feeding strategy except for fatter (P < .05) and lower (P < .06) yielding carcasses in cattle fed 5 vs 10% FM diets. Fish meal feeding increased (P < .01) concentrations of (n-3) FA, including EPA and DHA, and decreased (P < .05) concentrations of arachidonic acid. Increasing the amount of dietary FM further increased (P < .01) concentrations of EPA and DHA and decreased (P < .05) concentrations of (n-6) FA. We estimate that a 114-g steak from cattle fed 10% FM would supply 35 to 90% of the current average daily intake of EPA and DHA in North America. The results indicate that FM may have a role in niche marketing of beef provided that eating quality is not compromised.

Ten 394-kg, ruminally fistulated Hereford steers were used in a replicated 5 x 5 Latin square design to evaluate the effects of source and level of dietary NDF on chewing activities during eating and rumination. Diets contained 62 to 64% TDN and included 1) 80% pelleted concentrate (control; contained ground grains, fibrous byproducts, molasses, and protein, vitamin, and mineral supplements; 36% NDF, 16% CP) and 20% long timothy hay (67% NDF, 8% CP), 2) 80% control concentrate and 20% alfalfa cubes (56% NDF, 15% CP), 3) 90% control concentrate and 10% alfalfa cubes, 4) a completely pelleted diet using corn cobs as the primary NDF source (40% NDF, 17% CP), and 5) 80% textured (coarse instead of ground grains; 42% NDF, 15% CP) concentrate and 20% hay. Diets were formulated to be similar in NDF content, and dietary protein satisfied NRC recommendations. Chewing during eating did not differ (P greater than .10) between diets containing supplemental roughage but decreased (P less than .001) with the corn cob diet. Rumination chewing decreased (P less than .001) with the corn cob and cube diets. The number of chews per day during eating corrected for NDF intake/BW.75 decreased (P less than .05) in the corn cob diet. Rumination periods and duration increased and latency before rumination decreased in hay diets. Steers fed the corn cob diet tended to be more (P less than .10) consistent in time spent eating across 4-h intervals than steers fed the traditional diet. Replacement of long hay with the completely pelleted corn cob diet decreased rumination activity.

The effects of source and level of dietary NDF on intake, ruminal digestion in situ, ruminal fermentation, and total tract digestion were evaluated in Hereford steers using a replicated 5 x 5 Latin square design. Diets contained 62 to 64% TDN and included 1) 80% control concentrate (contained pelleted ground grains) and 20% timothy hay (traditional diet), 2) 80% control concentrate and 20% alfalfa cubes, 3) 90% control concentrate and 10% cubes, 4) a completely pelleted diet using corn cobs as the primary NDF source, and 5) 80% textured (rolled instead of ground grains) concentrate and 20% hay. Dry matter intake differed (P less than .05) between the traditional and cube diets due to limited acceptance of alfalfa cubes. Increased (P less than .05) ruminal osmolality, total VFA, and NH3 N and lower (P less than .01) ruminal pH in steers fed corn cob and cube diets relative to steers fed the traditional diet were due to preferential consumption of concentrate over supplemental roughage and the resultant rapid fermentation of concentrates. Potentially degradable DM in the traditional diet exceeded (P less than .06) all other diets, resulting in the increased (P less than .10) extent of DM disappearance despite a slower (P less than .05) rate of DM disappearance. Rate of NDF disappearance and all in situ starch disappearance parameters were similar between the traditional, corn cob, and cube diets. All ruminal digestion parameters involving NDF disappearance were similar between hay diets and between cube diets, whereas rate and extent of starch disappearance differed (P less than .05) between hay diets. Although formulation of diets with different sources of dietary NDF did not affect total tract digestion of nutrients, nutrient availability and ruminal fermentation were altered due to dietary differences in sources of dietary NDF and preferential selection of feedstuffs by steers.

Of the various factors that determine the choice of psychotropic drugs, economic cost to patients is often not adequately considered. The authors present results of a small survey of private pharmacy prices for psychopharmacologic agents, and compare these prices with wholesale prices and prices from alternative retail sources. They report a large degree of price variability, which sometimes even overshadows price differences between trade and generic preparations. Problems with bioequivalency among different preparations, the ramifications of prescribing the largest possible dose unit, and other issues related to drug cost are discussed.