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Conventional jet fuels derived from fossil sources contribute to greenhouse gas emissions and air pollution, leading to climate change. Recent studies have shown that biobased jet fuels from different feedstocks offer a more sustainable alternative to conventional fuels as they are derived from renewable biomass, reducing greenhouse gas emissions. The major feedstocks reviewed are jatropha curcas, camelina, karanja oil, waste cooking oil, and municipal solid waste. They offer diverse benefits for sustainable aviation fuel development. As a comparative analysis, this review examined jet fuel characteristics based on their physicochemical properties, namely energy content, viscosity, calorific value, cetane number, and freezing and flash points. The objective was to understand the influence of the properties on performance evaluation, environmental impact, and combustion characteristics. The properties of biojet fuels are compared with their fossil counterparts to validate their suitability as renewable alternatives and their benefits in terms of emissions reduction and engine performance. Biojet fuels perform better in terms of lower sulfur content, lower soot content, and a lower freezing point, their aromatic content, and their high cetane number. This study enhances the understanding of biojet fuels and their quality, and supports the development of sustainable fuel options. Overall, adherence to the American Society for Testing and Materials (ASTM) D7566‐18 standard is crucial for the acceptance and integration of biojet fuels into the aviation sector. Future research should explore feedstocks such as wood biomass, wastepaper, and agricultural residues for biojet fuels. It should also investigate the combustion and emission characteristics of biosourced aviation fuel at higher blending ratios (>50% by volume) with fossil Jet A‐1. [ABSTRACT FROM AUTHOR]

Due to the beneficial effects of natural antioxidants, such as the essential oils of different plants, including retarding or preventing the oxidation of oil/fat -based foods, as compared to the synthetic ones, they have received much attention. In this study, the effect of the essential oils of chaville, rosemary and lavender (100, 300 and 500 ppm) extracted by the steam distillation method on the thermal stability of camelina oil extracted by the cold press method under accelerated conditions (storage at 65 °C for 14 days) compared to camelina oil containing the synthetic antioxidant TBHQ was investigated. Data were analyzed by one -way analysis of variance (ANOVA) using SPSS software version 22 and the means were compared by the Duncan multiple range test. The results showed that the type of essential oil, storage time as well as their interactive effect had a significant (p<0.01) effect on the peroxide, anisidine and TOTOX values, as their values increased significantly (p<0.05) with increasing storage time and decreased significantly (p< 0.05) as the concentration of essential oil increased. After 14 days of storage under accelerated conditions, the total oxidation value (TOTOX value), when using chaville and rosemary essential oils (500 ppm), indicated that the oxidative stability of camelina oil increased compared to the camelina oil containing synthetic antioxidant TBHQ, and that it was suitable for frying. [ABSTRACT FROM AUTHOR]

After successfully performing Agrobacterium-mediated CRISPR-Cas9-based gene editing in plants, isolation of the Cas9 T-DNA is essential for the stable inheritance of induced mutations. Here, we report a simple technique that allows the isolation of Cas9-free mutants, eliminating the need for outcrossing or other intricate methods. This method is based on the ability of Basta-sensitive Arabidopsis thaliana seedlings, which generally perish, to recover and grow once transplanted to Basta-free growth media. By growing gene-edited heterozygous populations of single-locus insertion Basta-resistant plants on Basta selection media, plants lacking the Cas9 T-DNA can be identified. These pale-looking plants lacking Cas9 are then rescued on media lacking the Basta to recover Cas9-free plants. The ability of seedlings to recover from Basta selection was also studied in camelina, canola, and wheat. All three crops showed different recovery rates, with wheat demonstrating the highest recovery once transplanted from Basta to normal growth media. In summary, our findings demonstrate that by harnessing the recovery capability of Basta-sensitive seedlings, we can effectively identify and rescue plants lacking the Cas9 T-DNA, enabling the isolation of Cas9-free mutants in Arabidopsis and potentially extending to other crops. [ABSTRACT FROM AUTHOR]

Background: The bZIP transcription factor family, characterized by a bZIP domain, plays vital roles in plant stress responses and development. While this family has been extensively studied in various plant species, its specific functions in Camelina sativa (False Flax) remain underexplored. Methods and results: This study identified 71 bZIP transcription factors in C. sativa, classified into nine distinct groups based on phylogenetic analysis. Subcellular localization predicted a nucleus-specific expression for these bZIPs. Analysis of GRAVY scores revealed a range from 0.469 to -1.256, indicating a spectrum from hydrophobic to hydrophilic properties. Motif analysis uncovered 10 distinct motifs, with one motif being universally present in all CsbZIPs. Conserved domain analysis highlighted several domains beyond the core bZIP domain. Protein-protein interaction predictions suggested a robust network involving CsbZIPs. Moreover, promoter analysis revealed over 60 types of cis-elements, including those responsive to stress. Expression studies through RNA-seq and Real-time RT-qPCR demonstrated high expression of CsbZIPs in roots, leaves, flowers, and stems. Specifically, CsbZIP01, CsbZIP02, CsbZIP44, and CsbZIP60 were consistently up-regulated under cold, salt, and drought stresses, whereas CsbZIP34 and CsbZIP35 were down-regulated. Conclusion: This study presents the first comprehensive genome-wide profiling of bZIP transcription factors in Camelina sativa, providing novel insights into their roles in plant development and stress response mechanisms. By identifying and characterizing the bZIP gene family in C. sativa, this research offers new opportunities for improving stress tolerance and crop resilience through targeted genetic approaches, addressing key challenges in agriculture under changing environmental conditions. [ABSTRACT FROM AUTHOR]

Summary: Camelina (Camelina sativa L.), a hexaploid member of the Brassicaceae family, is an emerging oilseed crop being developed to meet the increasing demand for plant oils as biofuel feedstocks. In other Brassicas, high oil content can be associated with a yellow seed phenotype, which is unknown for camelina. We sought to create yellow seed camelina using CRISPR/Cas9 technology to disrupt its Transparent Testa 8 (TT8) transcription factor genes and to evaluate the resulting seed phenotype. We identified three TT8 genes, one in each of the three camelina subgenomes, and obtained independent CsTT8 lines containing frameshift edits. Disruption of TT8 caused seed coat colour to change from brown to yellow reflecting their reduced flavonoid accumulation of up to 44%, and the loss of a well‐organized seed coat mucilage layer. Transcriptomic analysis of CsTT8‐edited seeds revealed significantly increased expression of the lipid‐related transcription factors LEC1, LEC2, FUS3, and WRI1 and their downstream fatty acid synthesis‐related targets. These changes caused metabolic remodelling with increased fatty acid synthesis rates and corresponding increases in total fatty acid (TFA) accumulation from 32.4% to as high as 38.0% of seed weight, and TAG yield by more than 21% without significant changes in starch or protein levels compared to parental line. These data highlight the effectiveness of CRISPR in creating novel enhanced‐oil germplasm in camelina. The resulting lines may directly contribute to future net‐zero carbon energy production or be combined with other traits to produce desired lipid‐derived bioproducts at high yields. [ABSTRACT FROM AUTHOR]

Camelina (Camelina sativa) elite germplasm C17-833 was developed at the Agriculture and Agri-Food Canada Saskatoon Research and Development Centre. It was developed through hybridization of camelina cultivar AAC 10CS0048 and F4 line 11CS0231-24-10 and subsequent pedigree selection. C17-833 yields significantly higher (114%) than the check cultivar AAC 10CS0048. It also has significantly higher seed oil content (42.6% vs. 41.9%) and significantly larger seeds (126%). C17-833 has very good resistance to downy mildew disease caused by Hyalonospora camelinae and is adapted to all soil zones of the Canadian Prairies. [ABSTRACT FROM AUTHOR]

Oilseed plants are a valuable group of foods in human nutrition. Uncommon oilseed plants, such as safflower (Carthamus tinctorius L.), camelina (Camelina sativa L.), and black cumin (Nigella sativa L.), have been gaining increasing attention. The unique chemical compositions of these plants, which contribute to potential health benefits, underscore the importance of further exploration and study. The continuation of development of research in this field, resulting in the well-documented positive impact of these plants on human health, may lead to the possibility of them becoming new functional foods in the future. This review aims to present the potential health-promoting properties of the aforementioned uncommon oilseed plants based on recent literature reports and intends to inspire further exploration of their unique features. Among others, recent reports on the anticancer, antioxidant, and antibacterial potential, as well as the preventive potential in the case of metabolic diseases of these plants, were discussed. The importance of further development of these problems was also emphasized. [ABSTRACT FROM AUTHOR]

ABSTRACT Camelina sativa is regarded as a low‐input oilseed crop for versatile food, biofuels and industrial applications with potential production on marginal lands, whereas phosphate (Pi) deficiency greatly reduces camelina seed production. To improve camelina resilience to low P conditions, here we overexpressed the Pi deficiency–induced non‐specific phospholipase C4 (NPC4) to test its effect on camelina seed production under different levels of Pi availability. NPC4–overexpressing (OE) plants displayed increased seed yield and oil production, with a greater magnitude of increases under Pi‐deficient than Pi‐sufficient conditions. NPC4‐OE camelina had a higher level of total P and free Pi in young leaves but a lower level in old leaves than in wild‐type plants. More Pi was moved from old leaves to young leaves in NPC4‐OE than in wild‐type plants. NPC4‐OE increased the expression of Pi transporter genes, and the increase was greater in old leaves and under Pi‐deficient conditions. These data indicate that NPC4 improves camelina growth by promoting Pi remobilization from old to young tissues, revealing a mechanism by which NPC4 mediates plant response to Pi deficiency. [ABSTRACT FROM AUTHOR]

Electromagnetic waves are one of the tensions around agricultural plants, which have recently been widely used due to the development of telecommunication technology. Therefore, a field experiment was conducted during the years 2020-2021 at the Research Farm of Tarbiat Modares University to investigate the effect of WiFi electromagnetic wave (WEW) on the seed germination and growth characteristics of camelina. Seeds were exposed to Wi-Fi electromagnetic radiation at 15 cm (ER 15) and 25 cm (ER 25) cm from the modem for 24 hours (Pre-sowing treatment). The results showed that the germination percentage of seeds treated with WEW decreased by 24%. and this decrease was observed among growth factors such as hypocotyl length (20-43%), number of siliques per plant (56%), thousand seed weight (43%). Conversely, the root length, plant height, seed per silique and dry plant weight were increased by 24.6, 60.9, 10.5 and 56.3% under WEW treatment, respectively. Overall, this study showed that grain yield was greatly affected by electromagnetic waves and increased by about 23.45%. In general, among all the growth parameters, the correlation of the GY was positive and significant with DPW (r=0.735*), PH (r=0.669*) and SPS (r=0.659*). This result highlights the necessity for a better understanding of the mechanisms of electromagnetic waves in crops to help better seedling establishment. [ABSTRACT FROM AUTHOR]

Water stress is one of the most important limiting abiotic factors for crop plants. The drought resistance was investigated for 35 camelina doubled haploid lines along with a check cultivar (Sohail), under two irrigation conditions (supplementary irrigation and rainfed), during the crop year 2020-2021. The study was conducted in the randomized complete block design by three replications in three regions of Eslamabad-e Gharb, Mahidasht and Mehrgan. The indices of stress sensitivity (SSI), tolerance (Tol), drought resistance (DTI), mean productivity (MP), geometric mean productivity (GMP), harmonic mean (MH), yield stability (YSI) and drought resistance (DI) were calculated using seed yield in two environments of rainfed (Ys) and supplementary irrigation (Yp). There was a significant difference among regions for YS, Yp, MP, GMP, MH, and DTI indices. MP, GMP, MH, DTI and DI indices had significant correlations with two environments. According to MH, MP and GMP, the highest and the lowest drought resistance were observed in the Mehrgan region and Islamabad region, respectively. Mahidasht showed the highest value of drought resistance and Mehrgan showed the lowest value of drought resistance among the lines based on DTI. In addition, the results of the mean comparison, cluster analysis, principal components analysis and the three-dimensional scatter diagram of the lines based on the yield of two environments and the MP index showed that lines 1, 2, 3, 5, 6, 10, 7 and the check cultivar had more drought resistant. These lines had high seed yield in two contrast environments and showed more drought resistance, which is recommendable for breeding programs in the climatic conditions of Kermanshah province. [ABSTRACT FROM AUTHOR]

Abstract Seed triacylglycerol (TAG), a major component of vegetable oil, consists of a glycerol esterified with three fatty acids. Vegetable oil has industrial applications and is widely used as edible oil. The increasing demand for plant oils, owing to population growth, it is crucial to enhance the oil content in seeds. We found castor WRINKLED1A (RcWRI1A) and R2R3-type MYB domain protein 306 (RcMYB306) which have homology with Arabidopsis WRI1 (AtWRI1) and AtMYB96 which regulate genes involved in fatty acid and TAG synthesis, respectively. These castor genes were separately and jointly overexpressed using seed-specific promoters in an oil crop, camelina (Camelina sativa). Overexpression of RcWRI1A, RcMYB306, or RcWRI1A + RcMYB306 increased the total seed oil content in camelina. However, this increase was not significantly different from that observed during the overexpression of RcWRI1A or/and RcMYB306. RcWRI1A overexpression increased the fatty acid content, including 16:0, 18:2, 18:3. Contrastingly, RcMYB306 overexpression increased the 18:1, 18:2, 18:3, 20:0 and 20:1 fatty acid. In the RcWRI1A + RcMYB306 lines, changes in fatty acid composition demonstrated the combined effects of these transcription factors. These results suggest that RcWRI1A and RcMYB306 can be used to improve the productivity of oil crops.

Abstract In recent decades, the food system has been faced with the significant problem of increasing food waste. Therefore, the feed industry, supported by scientific research, is attempting to valorise the use of discarded biomass as co-products for the livestock sector, in line with EU objectives. In parallel, the search for functional products that can ensure animal health and performances is a common fundamental goal for both animal husbandry and feeding. In this context, camelina cake (CAMC), cardoon cake (CC) and cardoon meal (CM), due valuable nutritional profile, represent prospective alternatives. Therefore, the aim of this work was to investigate the antioxidant activity of CAMC, CC and CM following in vitro digestion using 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), Ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assays. Total phenolic content (TPC) and angiotensin converting enzyme (ACE) inhibitory activity, actively involved in modulating antioxidant properties, were also studied. Further, a peptidomic analysis was adopted to substantiate the presence of bioactive peptides after in vitro digestion. The results obtained confirmed an interesting nutritional profile of CAMC, CC and CM and relevant antioxidant and ACE inhibitory activities. In particular, considering antioxidant profile, CM and CC revealed a significantly higher (10969.80 ± 18.93 mg TE/100 g and 10451.40 ± 149.17 mg TE/100 g, respectively; p

Seed triacylglycerol (TAG), a major component of vegetable oil, consists of a glycerol esterified with three fatty acids. Vegetable oil has industrial applications and is widely used as edible oil. The increasing demand for plant oils, owing to population growth, it is crucial to enhance the oil content in seeds. We found castor WRINKLED1A (RcWRI1A) and R2R3-type MYB domain protein 306 (RcMYB306) which have homology with Arabidopsis WRI1 (AtWRI1) and AtMYB96 which regulate genes involved in fatty acid and TAG synthesis, respectively. These castor genes were separately and jointly overexpressed using seed-specific promoters in an oil crop, camelina (Camelina sativa). Overexpression of RcWRI1A, RcMYB306, or RcWRI1A + RcMYB306 increased the total seed oil content in camelina. However, this increase was not significantly different from that observed during the overexpression of RcWRI1A or/and RcMYB306. RcWRI1A overexpression increased the fatty acid content, including 16:0, 18:2, 18:3. Contrastingly, RcMYB306 overexpression increased the 18:1, 18:2, 18:3, 20:0 and 20:1 fatty acid. In the RcWRI1A + RcMYB306 lines, changes in fatty acid composition demonstrated the combined effects of these transcription factors. These results suggest that RcWRI1A and RcMYB306 can be used to improve the productivity of oil crops. [ABSTRACT FROM AUTHOR]

The article focuses on emission analysis of non-CO2 pollutions from aircraft engines on different flight levels: FL240, FL300 and FL350. The calculation was made based on the A320 flight from Berlin to Lisbon at flight level 350, which was the reference flight level in the analysis. Four sustainable aviation fuels have been taken into consideration: biofuel from jatropha and biofuel from camelina, which are used in different percentages of fuel: 20% of CSPK and JSPK and 40% of CSPK and JSPK. The results showed that the lowest emission of carbon monoxide is on the lowest tested flight level for flight on biofuel, and the lowest emission of nitrogen oxides is for Jet A-1 on the lowest tested flight level. Emission of every toxic gas compound has been compared to conventional jet fuel on flight level 350 to show the differences between flight levels. [ABSTRACT FROM AUTHOR]

Winter camelina [Camelina sativa (L.) Crantz] is a potential third crop to diversify maize (Zea mays L.)–soybean [Glycine max (L.) Merr.] rotations in the upper Midwest. Although generally considered a low‐input crop, empirical evidence suggests that it responds to added nitrogen (N) fertilization. However, optimum agronomic N rates have not been extensively studied in the region. A study was conducted from fall 2018 to fall 2020 at three locations to assess the effects of N fertilizer application time (all N‐rate applied in spring and N‐rate split applied in fall and spring) and rates on biomass and seed yield, and quality of winter camelina. Nitrogen application time did not affect yields. Both biomass and seed yields were greatly affected by N rates at all locations. Nitrogen had minimal effects on the oil and protein content of seeds, although greater N rates were associated with a slight decrease in oil content and a slight increase in protein content. The number of branches and silicles per plant varied significantly with N rates in all locations. The seed‐to‐silicle ratio showed significant differences in two out of three locations. Residual soil N increased with increasing N rates. A fertilization rate of 67 kg ha−1 provided the highest camelina seed yield. While this study has determined the agronomic maximum rate for applied N, further economic analysis could provide comprehensive decision‐making for farmers. Core Ideas: Fertilization timing did not affect winter camelina yield, but N rates did at all study locations.Greater N rates slightly reduced oil but increased protein in seed.Nitrogen rates affected the number of branches and silicles per plant at all study locations.A N rate of 67 kg ha−1 provided the maximum observed seed yield for winter camelina. [ABSTRACT FROM AUTHOR]

The aim of this study was to see whether it is possible to add camelina oil and seeds as ingredients in muffins in order to enhance their health-promoting value, such as their bioactive compound content, while maintaining the organoleptic attributes considered desirable by consumers. Camelina oil is characterised by a high linolenic acid content. Four types of muffins were prepared for analysis: MBnO—control muffins (containing 11.85% rapeseed oil), MCsO—muffins containing camelina oil instead of rapeseed oil, MCsS—muffins containing 6.65% camelina seeds in relation to the mass of prepared dough, and MCsOS—muffins containing both camelina oil and camelina seeds. The change in the fatty acid profile in muffins with the addition of camelina oil was significant; however, it was found that, as a result of thermal treatment, lower amounts of saturated fatty acids were formed. Among all the investigated experimental variants, muffins were characterised by the highest contents of all the phenolic acids analysed. The substitution of rapeseed oil with camelina oil had no negative effect on most of the organoleptic attributes of the muffins. Moreover, thanks to a greater content of carotenoids, camelina oil had an advantageous effect on the improvement of product colour, thus improving its overall desirability. [ABSTRACT FROM AUTHOR]

In recent decades, the food system has been faced with the significant problem of increasing food waste. Therefore, the feed industry, supported by scientific research, is attempting to valorise the use of discarded biomass as co-products for the livestock sector, in line with EU objectives. In parallel, the search for functional products that can ensure animal health and performances is a common fundamental goal for both animal husbandry and feeding. In this context, camelina cake (CAMC), cardoon cake (CC) and cardoon meal (CM), due valuable nutritional profile, represent prospective alternatives. Therefore, the aim of this work was to investigate the antioxidant activity of CAMC, CC and CM following in vitro digestion using 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), Ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assays. Total phenolic content (TPC) and angiotensin converting enzyme (ACE) inhibitory activity, actively involved in modulating antioxidant properties, were also studied. Further, a peptidomic analysis was adopted to substantiate the presence of bioactive peptides after in vitro digestion. The results obtained confirmed an interesting nutritional profile of CAMC, CC and CM and relevant antioxidant and ACE inhibitory activities. In particular, considering antioxidant profile, CM and CC revealed a significantly higher (10969.80 ± 18.93 mg TE/100 g and 10451.40 ± 149.17 mg TE/100 g, respectively; p < 0.05) ABTS value than CAMC (9511.18 ± 315.29 mg TE/100 g); a trend also confirmed with the FRAP assay (306.74 ± 5.68 mg FeSO4/100 g; 272.84 ± 11.02 mg FeSO4/100 g; 103.84 ± 3.27 mg FeSO4/100 g, for CC, CM and CAMC, respectively). Similar results were obtained for TPC, demonstrating the involvement of phenols in modulating antioxidant activity. Finally, CAMC was found to have a higher ACE inhibitory activity (40.34 ± 10.11%) than the other matrices. Furthermore, potentially bioactive peptides associated with ACE inhibitory, anti-hypertensive, anti-cancer, antimicrobial, antiviral, antithrombotic, DPP-IV inhibitory and PEP-inhibitory activities were identified in CAMC. This profile was broader than that of CC and CM. The presence of such peptides corroborates the antioxidant and ACE profile of the sample. Although the data obtained report the important antioxidant profile of CAMC, CC, and CM and support their possible use, future investigations, particularly in vivo trials will be critical to evaluate and further investigate their effects on the health and performance of farm animals. [ABSTRACT FROM AUTHOR]

Background: Tubulins play crucial roles in numerous fundamental processes of plant development. In flowering plants, tubulins are grouped into α-, β- and γ-subfamilies, while α- and β-tubulins possess a large isotype diversity and gene number variations among different species. This circumstance leads to insufficient recognition of orthologous isotypes and significantly complicates extrapolation of obtained experimental results, and brings difficulties for the identification of particular tubulin isotype function. The aim of this research is to identify and characterize tubulins of an emerging biofuel crop Camelina sativa. Results: We report comprehensive identification and characterization of tubulin gene family in C. sativa, including analyses of exon-intron organization, duplicated genes comparison, proper isotype designation, phylogenetic analysis, and expression patterns in different tissues. 17 α-, 34 β- and 6 γ-tubulin genes were identified and assigned to a particular isotype. Recognition of orthologous tubulin isotypes was cross-referred, involving data of phylogeny, synteny analyses and genes allocation on reconstructed genomic blocks of Ancestral Crucifer Karyotype. An investigation of expression patterns of tubulin homeologs revealed the predominant role of N6 (A) and N7 (B) subgenomes in tubulin expression at various developmental stages, contrarily to general the dominance of transcripts of H7 (C) subgenome. Conclusions: For the first time a complete set of tubulin gene family members was identified and characterized for allohexaploid C. sativa species. The study demonstrates the comprehensive approach of precise inferring gene orthology. The applied technique allowed not only identifying C. sativa tubulin orthologs in model Arabidopsis species and tracking tubulin gene evolution, but also uncovered that A. thaliana is missing orthologs for several particular isotypes of α- and β-tubulins. [ABSTRACT FROM AUTHOR]

Camelina sativa, commonly referred to as camelina or false flax, has emerged as a promising cover crop with the potential to mitigate climate change--a pressing global challenge that demands urgent and sustainable solutions. Belonging to the Brassicaceae family and native to Europe and Central Asia, camelina is an oilseed crop known for its resilience in diverse climates, including arid and semiarid regions, making it adaptable to various environments. A breeding program started from a study of six winter varieties and five spring varieties of camelina is described: these genetic materials were characterized by SSRs molecular markers and by GBS technique. Molecular data clearly showed all spring varieties were genetically similar and distinguishable from the winter varieties, which, in turn, clustered together. Using molecular data, parental varieties belonging to the two different clusters were selected to generate new genetic variability. The new variety obtained, selected through the bulk method based on three parameters: yield, earliness, and weight of 1000 seeds, has allowed the generation of the new genetic material provisionally named C1244. Chemical characterization was performed (bromatological and glucosinolates analysis) to better describe C1244 in comparison with benchmark varieties. The new variety exhibited early maturity, similar to spring varieties, making this genetic material promising for use in intercropping systems, a high weight of 1000 seeds (1.46 g) which improves and facilitates seeding/harvesting operations and a high oil content (33.62%) akin to winter varieties making it valuable for human and animal food purposes. [ABSTRACT FROM AUTHOR]

With increasing global attention toward the need for mitigating climate change, the transition to sustainable energy sources has become an essential priority. Introducing alternative oilseed crops, such as camelina (Camelina sativa L.), into intercropping systems with staple food crops can mitigate ILUC (indirect land use change) and their negative impact on biofuel production. The present study compared camelina + field pea intercropping (ICw + IP, winter sowing) and camelina + lentil intercropping (ICs + IL, spring sowing) with their respective single crops regarding weed control, soil coverage, yields, and camelina seed quality (1000-seed weight, oil, and fatty acid composition). The comparison between different cropping systems was conducted using a one-way ANOVA. Both intercropping improved weed control at an early stage but no differences in soil coverage were found. Camelina seed yield was negatively affected by the presence of peas, whereas the pulse was unaffected. Conversely, camelina seed yield was not affected when intercropped with lentils while lentils reduced their yield in the intercropping. Furthermore, when camelina was intercropped with lentils, a significant increase was reported in 1000-seed weight and α -linolenic acid (C18:3) compared with the sole-camelina. However, both intercropping systems had a land equivalent ratio (LER, based on total seed yield at maturity) higher than one. Defining the best combination of crops and the optimal sowing and harvesting settings remain key to increasing the adoption of intercropping systems by farmers. [ABSTRACT FROM AUTHOR]

Camelina (Camelina Sativa) is a hexaploid dicotyledonous plant from the Brassicaceae family, which is very similar to the Arabidopsis. The number of camelina chromosomes is 40=2n. WRKY transcription factors are one of the most important gene families in plants that play an important role in regulating growth and development and response to various stresses. In this research, 224 WRKY genes were identified in the camelina plant by searching the database, and the chromosomal position, gene length, and conserved motifs were identified in the camelina plant based on the Arabidopsis. Also, in this study, in order to validate the research, 2 genes WRKY8 and WRKY57 under drought stress were investigated by the qPCR method. The results indicated that both the above-mentioned genes were strongly expressed under drought stress conditions in tolerant varieties compared to normal conditions, but the trend was opposite in sensitive varieties. This study has provided acceptable and valuable information for studying the evolution and function of the WRKY gene family in camelina. [ABSTRACT FROM AUTHOR]

Drought stress is one of the most important factors that reduce the yield of crops in arid and semi-arid regions, including the climatic conditions of Iran. The experiment was conducted as factorial based on completely randomized design with three replications in the research greenhouse of Ilam University in 2020. The experimental factors included irrigation at three levels (100, 60, and 30% of field capacity) and three accessions of camelina (Kermanshah, Sabzevar, and Shahrekord). The results showed that under the irrigation conditions of 30%, seed yield, biological yield, harvest index, plant height, number of capsules per plant, number of seeds per capsule, relative leaf water content, photosynthesis rate, transpiration rate, stomatal conductance and total chlorophyll respectively 69.4, 53.8, 33.9, 44.1, 72.9, 25.6, 43.8, 72.0, 62.9, 56.7, and 61.1% reduction compared to optimal irrigation they found There was no difference observed between the three populations investigated in different irrigation conditions in terms of the investigated traits. In addition, the results showed that the highest amount of camellia seed oil (39%) was obtained in Kermanshah massif under favorable irrigation conditions; which decreased by 40.8% under severe stress of dehydration. In general, it is possible to use camelina Kermanshah under conditions of moderate water stress for oil production. [ABSTRACT FROM AUTHOR]

Maize (Zea mays L.) and soybean [Glycine max (L.) Merr.] rotations in the upper Midwest are highly productive. However, these narrow rotations are followed by a long winter fallow period. Over time, this has contributed to the loss of agroecological functioning, including increased ground water pollution from nitrate-nitrogen (NO3–N). Winter camelina [Camelina sativa (L.) Crantz] is a third crop that could grow during this fallow period, but its nitrogen (N) use and efficiency are not well known. A study was conducted at three locations in the U.S. upper Midwest to determine the N uptake and use efficiency of winter camelina in response to applied N and N application timing. Agronomic efficiency (AE), internal efficiency (IE), and nitrogen recovery efficiency (NRE) tended to decrease with increasing N rates, especially beyond 67 kg N ha−1 in most instances. Total N uptake ranged from 34 to 176 kg ha−1 across N rates, and was on average 1.5 fold the applied rate. Based on the observed decline in N use efficiency with increasing N rates, an application rate of 67 kg N ha−1 appears to balance efficient N use, high yield, and lower environmental risk compared to higher N rates. [ABSTRACT FROM AUTHOR]

The medicinal-oil plant Camelina sativa (L.) Crantz belongs to the Brassicaceae family. Various experiments have shown that the plant has much fewer water requirements and more resistance to spring cold than other oilseeds, especially Rapeseed. In this study, 136 camelina double haploid lines from anther culture progeny (F1) of crosses of 27 selected parents from different countries were generated. After ensuring that the lines were double haploid, biochemical markers were used to investigate genetic diversity. In the biochemical markers section, grain oil content, grain protein and type of fatty acids were measured. The experiment was carried out based on a randomized complete block design with four replications. To determine fatty acids using chromatography, 18 types of fatty acids were identified in camelina seed oil. Genetic parameters including phenotypic and genotypic variation coefficients, heritability and genetic advance were estimated. In this study, the highest phenotypic and genotypic variation in fatty acids (C14:0-C16:1) were estimated. Also, the highest general heritability for fatty acids (96.49% for C20:0, 98.92% for C20:2 and 98.59% for C20:3) were assessed. In this reserach, two lines with values of 35.81-36.67% linolenic acid and four lines with values between 22.08-23% of linoleic acid were identified. Also, the ratio of omega 6 to omega 3 ranged between 0.479 0 and 0.759. [ABSTRACT FROM AUTHOR]

The depletion of traditional energy sources and the increase in the cost of energy resources have a negative impact on the determination of the cost of production of agricultural and industrial products, which leads to a decrease in its competitiveness at the international level. Thus, the main task of the state is to ensure the efficient use of its own fuel and energy reserves and to diversify the sources and routes of supply of energy resources. Ukraine has all the necessary prerequisites for the widespread introduction and application of advanced technologies for growing and processing biomass and energy crops. The development of bioenergy technologies will help solve the problem of providing the country with energy resources, improve the environmental situation in the regions and help increase the employment of the local population. The fundamentals of growing less common energy crops are considered, the works of both domestic and foreign scientists are analysed, including the selection of the optimal place and soil, the establishment of optimal types of promising sparsely distributed energy crops, as raw materials for the energy sector of the state. Particular attention is paid to the need to improve the elements of technology in order to achieve a greater increase in biomass. [ABSTRACT FROM AUTHOR]

This study examined the combined effect of torrefaction and microwave absorbers on improving biomass thermochemical characteristics and grindability for heat, power, and value-added products. Camelina straw in two grinds, ground (6.4 mm screen size) and chopped with biochar addition (0%, 10% and 20%), was torrefied in a bench-scale microwave reactor at torrefaction temperatures of 250 °C and 300 °C with residence times of 10, 15 and 20 min under inert conditions and nitrogen-activated. After torrefaction, the geometric mean particle and size distribution, moisture content, ash content, bulk and particle densities were determined, and the grinding performance values of torrefied ground and chopped with and without biochar were determined and compared with the raw camelina straw. The results showed that the geometric diameter decreased after torrefaction in both grinds. The specific energy required for grinding torrefied biomass decreased significantly with biochar addition, longer residence times, and increased torrefaction temperatures. Torrefied ground camelina straw with biochar after grinding had the lowest grinding energy of 34.30 kJ at 300 °C/20 min. The surface morphology by confocal laser scanning microscopy of torrefied camelina straw particles indicated that biochar addition (>10%) and a torrefaction temperature at 250 °C can create profound surface distortion, and beyond 300 °C, colossal surface damage and carbonized weight fractions were produced. [ABSTRACT FROM AUTHOR]

Microgreens are a new, rapidly growing group of foodstuffs. The decorative function of these is often accompanied by their use in traditional dishes. As microgreens are eaten at very early stages, when the development of the epidermis is at its minimum, the bioavailability of minerals will be found to be higher in microgreens then in mature vegetables. So, microgreens can be an excellent functional food, especially for mineral-deficient populations, although they can also be a source of contaminants such as heavy metals or nitrates and nitrites. The purpose of this study was to measure the levels of selected heavy metals (i.e., cadmium, arsenic, lead, chromium, aluminium, zinc, copper, cobalt, molybdenum, manganese, vanadium, boron, antimony, thallium, titanium and strontium), as well as nitrates and nitrites, in microgreens at various stage of vegetation, using uncommon oilseed plants like nigella—Nigella sativa L., safflower—Carthamus tinctorius L., and camelina—Camelina sativa L. The examined microgreens of rare oilseed plants may be a source of contaminants and nitrates. The mineral profile of these plants is mainly determined by their genotype. Microgreens' cultivation involves compliance with safety standards and replicable conditions to guarantee that the highest nutritional value is reached at the lowest possible contaminant level. [ABSTRACT FROM AUTHOR]

In this study, beetroots were osmotically dehydrated in sugar beet molasses. The input parameters of the drying process were varied: temperature (20 °C, 40 °C, and 60 °C), time (1 h, 3 h, and 5 h), and concentration of sugar beet molasses (40%, 60%, and 80%). Basic quality indicators were determined for the dried beetroot samples: dry matter content, water loss, solid gain, mineral and betaine content, and phenols and flavonoids, as well as antioxidant potential. After optimizing the results, favorable drying parameters were selected: temperature 60 °C, molasses concentration 70%, and processing time 5 h. According to the optimal drying conditions, the beetroots were dried and stored at 4 °C for 28 days. Half of the dried samples were coated with an edible biopolymer coating based on Camelina sativa oilcake, while the other half of the samples remained uncoated. The sustainability study aimed to confirm the effects of the biopolymer coating on the quality and sustainability of the osmotically dried beetroots. [ABSTRACT FROM AUTHOR]

Spray-drying is a commonly used method for producing powdered flavors, but the high temperatures involved often result in the loss of volatile molecules. To address this issue, our study focused on a novel approach: developing O/W Pickering emulsions with agri-food byproducts to encapsulate and protect D-limonene during spray-drying and storage. Emulsions formulated with lupin hull, lupin-byproduct (a water-insoluble protein–fiber byproduct derived from the production of lupin protein isolate), and camelina press-cake were subjected to spray-drying at 160 °C. The results revealed that these emulsions exhibited good stability against creaming. The characteristics of the dry emulsions (powders) were influenced by the concentration of byproducts. Quantitative analysis revealed that Pickering emulsions enhanced the retention of D-limonene during spray-drying, with the highest retention achieved using 3% lupin hull and 1% camelina press-cake. Notably, lupin-stabilized emulsions yielded powders with enhanced oxidative stability compared to those stabilized with camelina press-cake. Our findings highlight the potential of food-grade Pickering emulsions to improve the stability of volatile flavors during both processing and storage. [ABSTRACT FROM AUTHOR]

Summary: In this study, microwave (MW)‐assisted extraction was used to obtain mucilage from Camelina sativa seeds. MW pretreatment was applied for 0, 2.5, 5 and 7.5 min and then conventional heating was conducted (55 °C for 2 h). The extracted mucilage was spray dried. The MW‐assisted extraction method (2.02%–2.50%) had higher extraction yield for camelina seed mucilage than the conventional heating method (1.91%) (P < 0.05). The emulsifying properties of the mucilages were compared with those of gum Arabic and all emulsions displayed shear thinning behaviour (pseudoplastic, n < 1). The apparent viscosity of the conventional, 2.5, 5 and 7.5 min MW mucilage emulsions were 15.83, 14.48, 7.91 and 7.56 cP, respectively, at 10 s−1. MW pretreatment improved physical stability and resulted in a reduction in the droplet size of mucilage‐stabilised emulsions with increasing process time. The highest droplet size belonged to the conventional mucilage emulsion (d32, 4.43 μm), while the lowest belonged to the 7.5 min MW mucilage emulsion (d32, 2.08 μm) (P < 0.05). The results highlight the advantages of using MW pretreatment for mucilage extraction and the potential of using camelina seed mucilage as a natural emulsifier. [ABSTRACT FROM AUTHOR]

Gut microbiota impacts fish metabolism, nutrient utilization, and health. We know little about how temperature and diet interact with rainbow trout gut microbes. A total of 288 fish (average body weight: 45.6 g) fed four iso‐caloric, ‐lipidic, and ‐nitrogenous diets comprised crude protein (40%) and lipid (20%) manufactured as 100% animal‐based protein (AP) and blend of 50 fish oil (FO)/50 camelina oil (CO), 100% AP and 100% CO, 100% plant‐based protein (PP) and blend of 50 FO/50 CO or 100% PP and 100% CO at 14 or 18°C water temperature. Gut content was analyzed using 16S rRNA gene and shotgun sequencing. Alpha‐diversity did not change significantly. Regardless of diet, Tenericutes, Firmicutes, Proteobacteria, Spirochaetes, Bacteroidetes, and Actinobacteria dominated. At family level, Aeromonadaceae and Enterobacteriaceae dominated at 18°C, while Mycoplasmataceae dominated at 14°C. Moreover, genes relating to amino acid, carbohydrate, fat, and energy metabolisms and fatty acid biosynthesis significantly increased at 18°C. Functional profiles did not vary significantly among diets, except for a higher methionine and cysteine metabolism in fish fed plant ingredient compared animal diet, suggesting trout fed plant‐derived protein mixed with CO could be as effective as those fed fish meals mixed with 50/50 FO and CO. [ABSTRACT FROM AUTHOR]

The composition of a soil microbial community that is associated with novel rotation crops could contribute to an increased yield of subsequent crops and is an important factor influencing the composition of the rhizosphere microbiome. However, the effect of alternative dryland crops on soil microbial community composition is not clear in the northern Great Plains (NGP). The objective of this study, therefore, was to evaluate the effects of the oilseed crops Ethiopian mustard (Brassica carinata A.) or camelina (Camelina sativa L.) or a 10-species forage/cover crop (CC) mix and fallow on soil biological health. Phospholipid fatty acid (PLFA) analysis was used to characterize the microbial community structure. The results showed that the total bacterial PLFA proportion was significantly higher in camelina and fallow compared to CCs and carinata, whereas the total fungal proportion was significantly higher under a CC mix compared to camelina and fallow. The fungal-to-bacterial ratio was significantly higher in CCs (0.130) and carinata (0.113) compared to fallow (0.088). Fungi are often considered a good indicator of soil health, while bacteria are crucial in soil functions. The changes in specific microbial communities due to crop-related alterations might play a key role in the yield of subsequent crops. This study provides valuable insights into the effect of oilseeds, CCs, and fallow on microbial communities. [ABSTRACT FROM AUTHOR]

This study was conducted to evaluate the Al3+ tolerance of sixteen camelina genotypes and to use melatonin or nano-selenium to alleviate Al3+-induced stress. A Petri dish study indicated seedling root length was suitable for describing the dose–response of seedling growth with increased Al3+ concentrations. Based on GR50 (Al3+ concentration causing a 50% reduction in the seedling root length), CamK6 (232.0 mg L−1) and CamK2 (97.0 mg L−1) were the most Al3+-tolerant and -sensitive genotypes, respectively. Under Al3+ stress, CamK6 and CamK2 treated by melatonin (50 μM) or nano-Se (0.4 mg L−1) showed a similar plant height and seed yield plant−1 (CamK6: 123.6 ± 9.8 cm and 0.562 ± 0.62 g; CamK2: 109.2 ± 8.7 cm and 0.49 ± 0.5 g) as the controls (CamK6: 121.1 ± 10.2 cm and 0.554 ± 0.4 g; CamK2: 110.0 ± 9.8 cm and 0.5 ± 0.4 g), and the values were greater than for the Al3+-treated plants (CamK6: 96.4 ± 9.2 cm and 0.48 ± 0.34 g; CamK2: 97.3 ± 8.1 cm and 0.42 ± 0.31 g). The results showed that melatonin or nano-Se through modulating biochemical reactions (e.g., antioxidant enzyme) can alleviate Al3+-induced growth inhibition in camelina. This study suggested melatonin or nano-Se can alleviate Al3+-induced growth inhibition by maintaining seed yield and improving oil quality in camelina. [ABSTRACT FROM AUTHOR]

Exposure to electromagnetic radiation (ER) in the radio frequency is one of the most common and rapidly growing environmental factors affecting human. Therefore, given the increasing global growth of telecommunication towers and Wi-Fi waves, understanding the potential of positive or negative effects of Wi-Fi electromagnetic waves (WEW) on the quality and quantity parameters of plants is essential. An experiment was carried out during the years 2020-2021 at the Research Farm of Tarbiat Modares University to investigate the effect of WEW on grain yield, fatty acid composition (FA), oil content, and antioxidant properties of camelina. Seeds were subjected to Wi-Fi electromagnetic radiation at distances of 15 cm (ER15) and 25 cm (ER25) from the modem for 24 h. The results showed that electromagnetic waves significantly affected grain yield, increasing it by approximately 23.45%. Additionally, the amount of oil and protein, crucial components of camelina seeds, exhibited a respective increase of 6.91% and decrease of 2.9% under Wi-Fi radiation conditions. Total tocopherol decreased by 6.39%, while ß-T remained unaffected by Wi-Fi radiation. Furthermore, the quantity of sterols (brassicasterol, cholesterol, and campesterol) decreased significantly due to Wi-Fi radiation. Based on the fatty acid profile responses, the maximum content of polyunsaturated fatty acids (SPUFA) was obtained in ER15 treatment, representing an increase of approximately 1.62%. Consequently, these waves resulted in a decrease in saturated fatty acids (SSFA) and erucic acid, thereby increasing the quality of camelina oil. In conclusion, WEW pretreatment has potential to increase grain yield, oil percentage, and quality of camelina oil. [ABSTRACT FROM AUTHOR]

The negative effects of global warming are increasing worldwide and this increase is expected to continue. The camelina plant, which can be grown in marginal areas, stands out among other oilseed plants because it is drought-resistant and less costly. In the research, seeds belonging to 8 camelina genotypes, which were prominent in terms of oil rate, seed and oil yield, were used in the trial established in Bolu province, out of a total of 52 genotypes obtained from the seed bank of the United States Department of Agriculture, Agricultural Research Service and Germany. The study was conducted according to the Randomized Complete Block Design in Bolu Abant İzzet Baysal University, Faculty of Agriculture, Research and Application Field in the 2021-2022 production season. In the trait, each plot consisted of six rows with a row length of 3 m, a row spacing of 20 cm, and in row of 5 cm, with three replications. According to the research results; plant height 66.33-71.00 cm, number of branches 3.17-5.07 number plant-1, 1000 seed weight 1.10-1.24 g, seed yield 1095.4-1436.6 kg ha-1, oil content 36.63-40.37%, protein content 23.65-27.22%, oil yield 408.3-559.8 kg ha-1 and protein yield 279.0-391.3 kg ha-1. It was found that values between. According to the results obtained from the study, the K52 genotype in terms of seed yield, the K11 genotype in terms of oil rate and the K52 genotype in terms of protein rate came to the fore. [ABSTRACT FROM AUTHOR]

Camelina (Camelina sativa L. Crantz) is an oil and medicinal crop in the Brassicaceae family that possesses many good agronomic qualities, such as stress resistance, strong adaptation, and low water and fertilizer inputs. The objectives of the present study were to optimize somatic embryogenesis and evaluate the genetic fidelity of regenerated Camelina plants using RAPD markers and flow cytometry. The experiment was performed in a factorial form using a completely randomized design with two factors and three replications. The studied factors included two explants of hypocotyls and cotyledons and four different combinations of PGRs composed of NAA, BAP, 2,4-D, and Kin. The observation of many somatic embryonic developmental stages occurring simultaneously on an embryogenic callus, including the globular, heart-shaped, torpedo-shaped, and cotyledonary phases, suggested that camelina embryogenesis accurately reflects an unsynchronized process. The cotyledon explants cultured on MS + 0.3 mg L− 1 NAA + 0.7 mg L− 1 BAP and 1.0 mg L− 1 Kin showed the highest rates of somatic embryogenesis and plant regeneration. Using RAPD markers, genetic fidelity in mother plants and regenerated plants was assessed for the first time in camelina. Sixty-four well-resolved bands were amplified using nine primers, ranging from four to eleven bands, with an average of 7.11 bands per primer. Molecular investigation revealed that there was no genetic variation in either the mother plants or the regenerated plants. The study of regenerated and mother plants using flow cytometry revealed monomorphic patterns, confirming the stability of the ploidy level across plant regeneration. These findings are helpful for breeding and genetic engineering of camelina. Key message: Camelina somatic embryogenesis is an unsynchronized process. This is the first study on the genetic fidelity of regenerated camelina plants using RAPD markers and flow cytometry. [ABSTRACT FROM AUTHOR]

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Oilseeds such as camelina and rapeseed are cultivated primarily for their high-quality oil. The oil de-rived from both crops is characterized by a high content of unsaturated fatty acids, tocopherols, phenols, and vitamin E. The camelina and rapeseed oils are applied in various branches of industry including food, pharmaceutical, chemical, and for obtaining biofuels. These oilseeds synthesize a significant amount of protein. These oilseeds play a major role in addressing the growing issue of protein deficit in the diets of people worldwide because of their high protein content. Efforts in breeding are essential to develop genotypes with high yield and protein content, while also maintaining high oil content and ensuring adaptability to climatic changes. During 2022/2023, various genotypes of rapeseed and camelina were grown in the experimental fields of the Institute of Field and Vegetable Crops Novi Sad in order to select varieties with ideal attributes. Among these, genotype NS Svetlana exhibited the highest oil content of 44.85 %, whereas genotype NS Dunav demonstrated the highest protein content of 24%. While camelina generally presented lower oil content compared to rapeseed, Genotype III stood out with a maximum oil content of 37.82%. Camelina also displayed significantly higher protein content than rapeseed. Protein content in camelina reached 26.59% in the case of Genotype I.

Abstract Background Pennycress and camelina are two important novel biofuel oilseed crop species. Their seeds contain high content of oil that can be easily converted into biodiesel or jet fuel, while the left-over materials are usually made into press cake meals for feeding livestock. Therefore, the ability to manipulate the seed coat encapsulating the oil- and protein-rich embryos is critical for improving seed oil production and press cake quality. Results Here, we tested the promoter activity of two Arabidopsis seed coat genes, AtTT10 and AtDP1, in pennycress and camelina by using eGFP and GUS reporters. Overall, both promoters show high levels of activities in the seed coat in these two biofuel crops, with very low or no expression in other tissues. Importantly, AtTT10 promoter activity in camelina shows differences from that in Arabidopsis, which highlights that the behavior of an exogenous promoter in closely related species cannot be assumed the same and still requires experimental determination. Conclusion Our work demonstrates that AtTT10 and AtDP1 promoters are suitable for driving gene expression in the outer integument of the seed coat in pennycress and camelina.

Oilseed plants are a valuable group of foods in human nutrition. Uncommon oilseed plants, such as safflower (Carthamus tinctorius L.), camelina (Camelina sativa L.), and black cumin (Nigella sativa L.), have been gaining increasing attention. The unique chemical compositions of these plants, which contribute to potential health benefits, underscore the importance of further exploration and study. The continuation of development of research in this field, resulting in the well-documented positive impact of these plants on human health, may lead to the possibility of them becoming new functional foods in the future. This review aims to present the potential health-promoting properties of the aforementioned uncommon oilseed plants based on recent literature reports and intends to inspire further exploration of their unique features. Among others, recent reports on the anticancer, antioxidant, and antibacterial potential, as well as the preventive potential in the case of metabolic diseases of these plants, were discussed. The importance of further development of these problems was also emphasized.

Recently, the utilization and research of biodiesel has become increasingly popular due to its reduced emissions, lower cost, and potential for achieving energy independence. A promising application of biodiesel is in diesel engines, where it can be used as a substitute for traditional petroleum-based diesel fuel. Camelina sativa is an oil seed crop with prospective uses in biodiesel extraction due to its high crop harvest in a year, good net energy ratio, the considerable oil content in its seed, and lower oil extraction expenses. Biodiesel derived from camelina sativa L. is prepared via transesterification. In this study, the prepared biodiesel is blended with diesel at various proportions and is used in an engine to investigate its combustion performance and emission characteristics. From the results, it is evident that the CMB 20 blend (20% of camelina biodiesel and 80% of diesel) shows the better performance among all of the blends used. The brake thermal efficiency of CMB 20 is 23.45%, its specific fuel consumption is 0.355 kW/kg hr, and it also produced less emissions when compared to other blends. [ABSTRACT FROM AUTHOR]

Camelina is an oil seed crop that is enjoying increasing interest because it has a particularly valuable fatty acid profile, is modest regarding its water and nutrient requirements, and is comparatively resilient to abiotic and biotic stress factors. The regeneration of plants from cells accessible to genetic manipulation is an essential prerequisite for the generation of genetically engineered plants, be it by transgenesis or genome editing. Here, immature embryos were used on the assumption that their incomplete differentiation was associated with totipotency. In culture, regenerative structures appeared adventitiously at the embryos' hypocotyls. For this, the application of auxin- or cytokinin-type growth regulators was essential. The formation of regenerative structures was most efficient when indole-3-acetic acid was added to the induction medium at 1 mg/L, zygotic embryos of the medium walking stick stage were used, and their hypocotyls were stimulated by pricking to a wound response. Histological examinations revealed that the formation of adventitious shoots was initiated by locally activated cell division and proliferation in the epidermis and the outer cortex of the hypocotyl. While the regeneration of plants was established in principle using the experimental line Cam139, the method proved to be similarly applicable to the current cultivar Ligena, and hence it constitutes a vital basis for future genetic engineering approaches. [ABSTRACT FROM AUTHOR]

Camelina is an interesting crop and producers must adopt cultural practices to achieve the highest yield and oil content possible. Considering the size of the seed, the harvesting phase is crucial to reduce losses and maximize income. Furthermore, in recent years, with the worsening of climatic conditions due to global warming, crop management can no longer follow the classic seasonality but must adapt to new climatic conditions. The possibility of double cropping, that is the process of producing two crops in a single season, allows multiple advantages such as weed control, greater remuneration, and less exposure to bare soil which determine greater resilience of the production system. To enable this, especially in recent years, even a few days of difference in the cultivation phases can guarantee the success of double cropping. For these reasons, the authors compared two different harvesting strategies: direct combining at full maturity (DC); swathing + combining at full maturity (SW). The working performance, cost, and seed losses associated with each harvesting method were calculated. The results highlighted how SW reduced the crop cycle length by 11 days, did not influence seed losses and crop yield but showed lower performance and higher cost with respect to DC. [ABSTRACT FROM AUTHOR]

Camelina [Camelina sativa (L.) Crantz] is a Brassicaceae oilseed that is gaining interest worldwide as low‐maintenance crop for diverse biobased applications. One of the most important factors determining its productivity is climate. We conducted a bioclimate analysis in order to analyze the relationship between climatic factors and the productivity of spring‐type camelina seeded in the spring, and to identify regions of the world with potential for camelina in this scenario. Using the modelling tool CLIMEX, a bioclimatic model was developed for spring‐seeded spring‐type camelina to match distribution, reported seed yields and phenology records in North America. Distribution, yield, and phenology data from outside of North America were used as independent datasets for model validation and demonstrated that model projections agreed with published distribution records, reported spring‐seeded camelina yields, and closely predicted crop phenology in Europe, South America, and Asia. Sensitivity analysis, used to quantify the response of camelina to changes in precipitation and temperature, indicated that crop performance was more sensitive to moisture than temperature index parameters, suggesting that the yield potential of spring‐seeded camelina may be more strongly impacted by water‐limited conditions than by high temperatures. Incremental climate scenarios also revealed that spring‐seeded camelina production will exhibit yield shifts at the continental scale as temperature and precipitation deviate from current conditions. Yield data were compared with indices of climatic suitability to provide estimates of potential worldwide camelina productivity. This information was used to identify new areas where spring‐seeded camelina could be grown and areas that may permit expanded production, including eastern Europe, China, eastern Russia, Australia and New Zealand. Our model is the first to have taken a systematic approach to determine suitable regions for potential worldwide production of spring‐seeded camelina. [ABSTRACT FROM AUTHOR]

Young shoots are a completely new and rapidly growing group of foodstuffs. Also known as "vegetable confetti", they are a useful addition to commonly consumed meals and often serve a decorative purpose, especially when paired with traditional dishes. Most users are unaware of their invaluable properties as a source of bioactive compounds and add them simply as a dish garnish. Hence, the aim of this study is to evaluate and compare selected health quality parameters of the young shoots of rare oilseed plants (Nigella sativa L., Carthamus tinctorius L., and Camelina sativa L.), which have not been studied in the literature. They are examined for proximate composition (dry matter, total protein, crude fat, ash, digestible carbohydrates, dietary fiber), antioxidative properties (vitamin C, total carotenoids, and total polyphenol content), the content of sixteen selected minerals (calcium, potassium, magnesium, sodium, phosphorus, sulphur, selenium, barium, iron, lithium, beryllium, nickel, gallium, indium, bismuth, silver) as well as antioxidant activity at two harvest dates. The ready-to-eat young shoots in the phase of intensive growth are characterized by a very high content of the examined components and antioxidant properties, which differ depending on the harvest date and plant species. Significantly higher contents of protein, fat, and some minerals have been found in the young shoots from the first harvest compared to those from the second harvest. The antioxidant properties of the young shoots generally increase with maturity. It was not possible, however, to conclusively assess which species of young shoots show the highest health quality. [ABSTRACT FROM AUTHOR]

Camelina neglecta is a new diploid Brassicaceae species, which has great research value because of its close relationship with the hexaploid oilseed crop Camelina sativa. Here, we report a chromosome-level assembly of C. neglecta with a total length of 210 Mb. By adopting PacBio sequencing and Hi-C technology, the C. neglecta genome was assembled into 6 chromosomes with scaffold N50 of 29.62 Mb. C. neglecta has undergone the whole-genome triplication (γ) shared among eudicots and two whole-genome duplications (α and β) shared by crucifers, but it has not undergone a specific whole-genome duplication event. By synteny analysis between C. neglecta and C. sativa, we successfully used the method of calculating Ks to distinguish the three subgenomes of C. sativa and determined that C. neglecta was closest to the first subgenome (SG1) of C. sativa. Further, transcriptomic analysis revealed the key genes associated with seed oil biosynthesis and its transcriptional regulation, including SAD, FAD2, FAD3, FAE1, ABI3, WRI1 and FUS3 displaying high expression levels in C. neglecta seeds. The high representability of C. neglecta as a model species for Camelina-based biotechnology research has been demonstrated for the first time. In particular, floral Agrobacterium tumefaciens infiltration-based transformation of C. neglecta, leading to overexpression of CvLPAT2, CpDGAT1 and CvFatB1 transgenes, was demonstrated for medium-chain fatty acid accumulation in C. neglecta seed oil. This study provides an important genomic resource and establishes C. neglecta as a new model for oilseed biotechnology research. [ABSTRACT FROM AUTHOR]

Climate change has created unprecedented stresses in the agricultural sector, driving the necessity of adapting agricultural practices and developing novel solutions to the food crisis. Camelina sativa (Camelina) is a recently emerging oilseed crop with high nutrient-density and economic potential. Camelina seeds are rich in essential fatty acids and contain potent antioxidants required to maintain a healthy diet. Camelina seeds are equally amenable to economic applications such as jet fuel, biodiesel and high-value industrial lubricants due to their favorable proportions of unsaturated fatty acids. High soil salinity is one of the major abiotic stresses threatening the yield and usability of such crops. A promising mitigation strategy is automated, non-destructive, image-based phenotyping to assess seed quality in the food manufacturing process. In this study, we evaluate the effectiveness of image-based phenotyping on fluorescent and visible light images to quantify and qualify Camelina seeds. We developed a user-friendly web portal called SeedML that can uncover key morphocolorimetric features to accurately identify Camelina seeds coming from plants grown in high salt conditions using a phenomics platform equipped with fluorescent and visible light cameras. This portal may be used to enhance quality control, identify stress markers and observe yield trends relevant to the agricultural sector in a high throughput manner. Findings of this work may positively contribute to similar research in the context of the climate crisis, while supporting the implementation of new quality controls tools in the agrifood domain. [ABSTRACT FROM AUTHOR]

Introduction and Objective: Stimulants are compounds that initiate signals for cells to increase or decrease the production of secondary metabolites and plant defense response and stimulants such as Putrescine play a role in regulating various plant physiological processes. The use of nano-fertilizers has led to an increase in the efficiency of the use of nutrients, the correct management of fertilizer consumption, and a reduction in the frequency of fertilizer application. This research was conducted in order to investigate the effect of iron nanoparticle and putrescine as plant growth stimulants on the yield and yield components of the oil-medicinal plant Camelina. Material and Methods: This research was conducted as factorial design in a randomized complete block design with three replications in the research fields of Baye-Kala Agricultural Research Station (BARS) in Neka county in 2021. The studied factors included iron nanoparticles in four concentrations (zero, 20, 40, and 60 ppm) and polyamine putrescine in four concentrations (zero, 0.5, 1, and 1.5 mM). Results: The results of variance analysis of different traits indicated that the simple effect of iron nanoparticles and putrescine was significant on all the studied traits at the 5% level. Also, the interaction of the two factors was significant on all the studied traits except peroxidase in the reproductive stage and this interaction was nonsignificant on all physiological traits in vegetative stage. According to the results, the highest amount of flavonoid (21.871 and 21.389, respectively), soluble sugar (116.643 and 105.453, respectively), catalase (1.301 and 1.394, respectively) and peroxidase (5.056 and 4.687, respectively) were observed in the vegetative stage in the application of 40 ppm iron nanoparticles and 1.5 mM putrescine. The results indicated that the highest amount of flavonoid (40.72), soluble sugar (139.27) and plant height (115.75) were observed in the treatment combination of 60 ppm iron and 1 mM putrescine, the highest percentage of oil (41.76) and protein (27.77) were observed in the treatment combination of 40 ppm iron and 1.5 mM putrescine and the highest amount of grain yield (210.27) and morphological components of yield were observed in the treatment combination of 40 ppm iron and 1 mM putrescine. Conclusion: In general, the interaction effect of 40 ppm of iron nanoparticles and high concentration of putrescine had the best results, and the spraying of iron nanoparticles and putrescine through the more effective absorption of microelement nutrients and strengthening the plant's defense system can improve the growth, development and yield of the medicinal-oil Camelina plant. [ABSTRACT FROM AUTHOR]

Camelina, a traditional oil-producing plant, has gained global interest due to the high-quality oil found in its seeds. It has numerous applications, including human dietary consumption, aviation biofuel, and biodiesel production. Seed quality testing is crucial for identifying suitable seed batches for market sale. Currently, vigor tests have been established for a limited selection of commercially cultivated plant species, including camelina. This study aims to assess seed vigor and contribute to the development and validation of methods/tests for reliable vigor assessment. The experiment used two camelina genotypes developed at the Institute of Field and Vegetables Crops in Novi Sad, Serbia. The findings revealed a noteworthy reduction in germination percentages for both genotypes across all the conducted tests, as compared to the conventional laboratory germination. Simultaneously, there was a notable increase in abnormal seedlings. However, no statistically significant differences in the values of growth parameters were observed among the applied tests. In summary, the reduced seed vigor values indicate potential issues with this trait, despite generally sound germination. Additionally, the preliminary findings and methodology developed for testing the camelina seed vigor highlighted the need for optimization when applying these tests to other species to ensure their reliability and applicability. [ABSTRACT FROM AUTHOR]

The purpose of the study was to investigate the effects of dietary hempseed or camelina cakes on the fatty acid profiles of intramuscular fat in quail. A total of 189 one-day-old quail were allocated to three dietary treatment groups. The diet of the control (C) group was supplemented with 10% rapeseed cake, whereas the rapeseed in experimental 1 (HE) and experimental 2 (CA) groups was replaced by, respectively, hempseed cake and camelina cake in the same proportions. The length of the study was 42 days. Dietary enrichment with camelina cake increased the α-linolenic fatty acid (ALA) content in the meat of CA group 2.5 times (p < 0.01). The muscle tissues of CA contained 3.4–3 times more eicosapentaenoic acid (p < 0.01), 1.2 times more docosapentaenoic acid (p < 0.05–p < 0.01) and 1.3 times more docosahexaenoic acid (p < 0.01) and, thus, demonstrated the increase in total long chain (LC) n-3 polyunsaturated fatty acids (PUFA) (p < 0.01) and total n-3 PUFA (p < 0.01) compared with the C group. The ALA and total n-3 PUFA content in the breast and leg meat of HE-treated quail were, respectively, 1.3 and 1.1 times higher (p < 0.01) than in the C group but the accumulation was lower compared to the CA group. The content of γ-linolenic acid was found to be 1.21–1.31 times higher in HE quail meat (p < 0.01). However, hempseed cake supplementation had a negative effect on growth performance. The supplementation of quail feed with camelina or hempseed cakes resulted in the production of healthy meat with an increased n-3 PUFA content. [ABSTRACT FROM AUTHOR]