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194 results on '"Callister, Robert J."'

1. Calretinin-expressing islet cells are a source of pre- and post-synaptic inhibition of non-peptidergic nociceptor input to the mouse spinal cord

Author

Davis, Olivia C., Dickie, Allen C., Mustapa, Marami B., Boyle, Kieran A., Browne, Tyler J., Gradwell, Mark A., Smith, Kelly M., Polgár, Erika, Bell, Andrew M., Kókai, Éva, Watanabe, Masahiko, Wildner, Hendrik, Zeilhofer, Hanns Ulrich, Ginty, David D., Callister, Robert J., Graham, Brett A., Todd, Andrew J., and Hughes, David I.

Published
2023
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6. Calretinin-expressing islet cells: a source of pre- and post-synaptic inhibition of non-peptidergic nociceptor input to the mouse spinal cord

Author

Davis, Olivia C., primary, Dickie, Allen C., additional, Mustapa, Marami B., additional, Boyle, Kieran A., additional, Browne, Tyler J., additional, Gradwell, Mark A., additional, Smith, Kelly M., additional, Polgár, Erika, additional, Bell, Andrew M., additional, Kókai, Éva, additional, Watanabe, Masahiko, additional, Wildner, Hendrik, additional, Zeilhofer, Hanns Ulrich, additional, Ginty, David D., additional, Callister, Robert J., additional, Graham, Brett A., additional, Todd, Andrew J., additional, and Hughes, David I., additional

Published
2023
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8. Molecular and functional changes to postsynaptic cholinergic signaling in the vestibular sensory organs of aging C57BL/6 mice

Author

Poppi, Lauren A, primary, Bigland, Mark J, additional, Cresswell, Ethan T, additional, Tabatabaee, Hessam, additional, Lorincz, David, additional, Drury, Hannah R, additional, Callister, Robert J, additional, Holt, Joseph C, additional, Lim, Rebecca, additional, Brichta, Alan M, additional, and Smith, Doug W, additional

Published
2023
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9. Calretinin-expressing islet cells are a source of pre- and post-synaptic inhibition of non-peptidergic nociceptor input to the mouse spinal cord

Author

Davis, Olivia C, Dickie, Allen C, Mustapa, Marami B, Boyle, Kieran A, Browne, Tyler J, Gradwell, Mark A, Smith, Kelly M, Polgár, Erika, Bell, Andrew M, Kókai, Éva, Watanabe, Masahiko, Wildner, Hendrik, Zeilhofer, Hanns Ulrich; https://orcid.org/0000-0001-6954-4629, Ginty, David D, Callister, Robert J, Graham, Brett A, Todd, Andrew J, Hughes, David I, Davis, Olivia C, Dickie, Allen C, Mustapa, Marami B, Boyle, Kieran A, Browne, Tyler J, Gradwell, Mark A, Smith, Kelly M, Polgár, Erika, Bell, Andrew M, Kókai, Éva, Watanabe, Masahiko, Wildner, Hendrik, Zeilhofer, Hanns Ulrich; https://orcid.org/0000-0001-6954-4629, Ginty, David D, Callister, Robert J, Graham, Brett A, Todd, Andrew J, and Hughes, David I

Abstract

Unmyelinated non-peptidergic nociceptors (NP afferents) arborise in lamina II of the spinal cord and receive GABAergic axoaxonic synapses, which mediate presynaptic inhibition. However, until now the source of this axoaxonic synaptic input was not known. Here we provide evidence that it originates from a population of inhibitory calretinin-expressing interneurons (iCRs), which correspond to lamina II islet cells. The NP afferents can be assigned to 3 functionally distinct classes (NP1-3). NP1 afferents have been implicated in pathological pain states, while NP2 and NP3 afferents also function as pruritoceptors. Our findings suggest that all 3 of these afferent types innervate iCRs and receive axoaxonic synapses from them, providing feedback inhibition of NP input. The iCRs also form axodendritic synapses, and their targets include cells that are themselves innervated by the NP afferents, thus allowing for feedforward inhibition. The iCRs are therefore ideally placed to control the input from non-peptidergic nociceptors and pruritoceptors to other dorsal horn neurons, and thus represent a potential therapeutic target for the treatment of chronic pain and itch.

Published
2023

13. Altered Intrinsic Properties and Inhibitory Connectivity in Aged Parvalbumin-Expressing Dorsal Horn Neurons

Author

Gradwell, Mark A., Smith, Kelly M., Dayas, Christopher V., Smith, Douglas W., Hughes, David I., Callister, Robert J., and Graham, Brett A.

Subjects
Posterior Horn Cells, Mice, Spinal Cord Dorsal Horn, Cellular and Molecular Neuroscience, Parvalbumins, Hyperalgesia, Interneurons, Cognitive Neuroscience, Neuroscience (miscellaneous), Animals, Sensory Systems
Abstract

The incidence of pain symptoms such as allodynia are known to increase with age. Parvalbumin expressing interneurons (PVINs) within the dorsal horn (DH) of the spinal cord play an important role in allodynia whereby their inhibitory connections prevent innocuous touch information from exciting nociceptive pathways. Here we ask whether the functional properties of PVINs are altered by aging, comparing their functional properties in adult (3–7 month) and aged mice (23–28 month). Patch clamp recordings were made from PVINs in laminae IIi-III of parasagittal spinal cord slices. The intrinsic excitability of PVINs changed with age. Specifically, AP discharge shifted from initial bursting to tonic firing, and firing duration during current injection increased. The nature of excitatory synaptic input to PVINs also changed with age with larger but less frequent spontaneous excitatory currents occurring in aged mice, however, the net effect of these differences produced a similar level of overall excitatory drive. Inhibitory drive was also remarkably similar in adult and aged PVINs. Photostimulation of ChR2 expressing PVINs was used to study inhibitory connections between PVINs and unidentified DH neurons and other PVINs. Based on latency and jitter, monosynaptic PVIN to unidentified-cell and PVIN-PVIN connections were compared in adult and aged mice, showing that PVIN to unidentified-cell connection strength increased with age. Fitting single or double exponentials to the decay phase of IPSCs showed there was also a shift from mixed (glycinergic and GABAergic) to GABAergic inhibitory transmission in aged animals. Overall, our data suggest the properties of PVIN neurons in aged animals enhance their output in spinal circuits in a manner that would blunt allodynia and help maintain normal sensory experience during aging.

Published
2022

20. Diversity of inhibitory and excitatory parvalbumin interneuron circuits in the dorsal horn

Author

Gradwell, Mark A., primary, Boyle, Kieran A., additional, Browne, Tyler J., additional, Bell, Andrew M., additional, Leonardo, Jacklyn, additional, Peralta Reyes, Fernanda S., additional, Dickie, Allen C., additional, Smith, Kelly M., additional, Callister, Robert J., additional, Dayas, Christopher V., additional, Hughes, David I., additional, and Graham, Brett A., additional

Published
2021
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29. Calretinin positive neurons form an excitatory amplifier network in the spinal cord dorsal horn

Author

Smith, Kelly M, primary, Browne, Tyler J, additional, Davis, Olivia C, additional, Coyle, A, additional, Boyle, Kieran A, additional, Watanabe, Masahiko, additional, Dickinson, Sally A, additional, Iredale, Jacqueline A, additional, Gradwell, Mark A, additional, Jobling, Phillip, additional, Callister, Robert J, additional, Dayas, Christopher V, additional, Hughes, David I, additional, and Graham, Brett A, additional

Published
2019
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30. Effects Of treadmill training on hindlimb muscles of spinal cord–injured mice

Author

Battistuzzo, Camila R., Rank, Michelle M., Flynn, Jamie R., Morgan, David L., Callister, Robin, Callister, Robert J., and Galea, Mary P.

Subjects
muscle atrophy, Male, muscle fiber type, Basic and Translational Science, mice, Time Factors, hemisection, spinal cord injury, Hindlimb, Disease Models, Animal, Physical Conditioning, Animal, treadmill, Multivariate Analysis, Exercise Test, Animals, Muscle, Skeletal, Spinal Cord Injuries
Abstract

Introduction: Treadmill training is known to prevent muscle atrophy after spinal cord injury (SCI), but the training duration required to optimize recovery has not been investigated. Methods: Hemisected mice were randomized to 3, 6, or 9 weeks of training or no training. Muscle fiber type composition and fiber cross‐sectional area (CSA) of medial gastrocnemius (MG), soleus (SOL), and tibialis anterior (TA) were assessed using ATPase histochemistry. Results: Muscle fiber type composition of SCI animals did not change with training. However, 9 weeks of training increased the CSA of type IIB and IIX fibers in TA and MG muscles. Conclusions: Nine weeks of training after incomplete SCI was effective in preventing atrophy of fast‐twitch muscles, but there were limited effects on slow‐twitch muscles and muscle fiber type composition. These data provide important evidence of the benefits of exercising paralyzed limbs after SCI. Muscle Nerve, 2016 Muscle Nerve 55: 232–242, 2017

Published
2016

31. Crosstalk between mitochondria, calcium channels and actin cytoskeleton modulates noradrenergic activity of locus coeruleus neurons

Author

de Oliveira, Ramatis B., Petiz, Lyvia L., Lim, Rebecca, Lipski, Janusz, Gravina, Fernanda S., Brichta, Alan M., Callister, Robert J., Leão, Richardson N, van Helden, Dirk F., de Oliveira, Ramatis B., Petiz, Lyvia L., Lim, Rebecca, Lipski, Janusz, Gravina, Fernanda S., Brichta, Alan M., Callister, Robert J., Leão, Richardson N, and van Helden, Dirk F.

Abstract

Locus coeruleus (LC) is the name of a group of large sized neurons located at the brain stem, which provides the main source of noradrenaline to the central nervous system, virtually, innervating the whole brain. All noradrenergic signalling provided by this nucleus is dependent on an intrinsic pacemaker process. Our study aims to understand how noradrenergic neurons finely tune their pacemaker processes and regulate their activities. Here we present that mitochondrial perturbation in the LC from mice, inhibits spontaneous firing by a hyperpolarizing response that involves Ca2+ entry via L-type Ca2+ channels and the actin cytoskeleton. We found that pharmacological perturbation of mitochondria from LC neurons using the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP), induced a dominant hyperpolarizing response when electrophysiological approaches were performed. Surprisingly, the CCCP-induced hyperpolarizing response was dependent on L-type Ca2+ channel-mediated Ca2+ entry, as it was inhibited by: the removal of extracellular Ca2+; the addition of Cd2+; nifedipine or nicardipine; but not by the intracellular dialysis with the Ca2+ chelator 1,2-Bis(2-Aminophenoxy)ethane-N,N,N ',N '-tetraacetic acid, the latter indicating that the response was not because of a global change in [Ca2+](c) but does not exclude action at intracellular microdomains. Further to this, the incubation of slices with cytochalasin D, an agent that depolymerises the actin cytoskeleton, inhibited the hyperpolarizing response indicating an involvement of the actin cytoskeleton. The data are consistent with the hypothesis that there is a crosstalk between mitochondria and L-type Ca2+ channels leading to modulation of noradrenergic neuronal activity mediated by the actin cytoskeleton. Open Science Badges This article has received a badge for *Open Materials* because it provided all relevant information to reproduce the study in the manuscript. The complete Open Science Disclosure form fo

Published
2019
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33. Author response: Calretinin positive neurons form an excitatory amplifier network in the spinal cord dorsal horn

Author

Smith, Kelly M, primary, Browne, Tyler J, additional, Davis, Olivia C, additional, Coyle, A, additional, Boyle, Kieran A, additional, Watanabe, Masahiko, additional, Dickinson, Sally A, additional, Iredale, Jacqueline A, additional, Gradwell, Mark A, additional, Jobling, Phillip, additional, Callister, Robert J, additional, Dayas, Christopher V, additional, Hughes, David I, additional, and Graham, Brett A, additional

Published
2019
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34. Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia

Author

Boyle, Kieran A., primary, Gradwell, Mark A., additional, Yasaka, Toshiharu, additional, Dickie, Allen C., additional, Polgár, Erika, additional, Ganley, Robert P., additional, Orr, Desmond P.H., additional, Watanabe, Masahiko, additional, Abraira, Victoria E., additional, Kuehn, Emily D., additional, Zimmerman, Amanda L., additional, Ginty, David D., additional, Callister, Robert J., additional, Graham, Brett A., additional, and Hughes, David I., additional

Published
2019
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36. Different forms of glycine- and GABAA-receptor mediated inhibitory synaptic transmission in mouse superficial and deep dorsal horn neurons

Author

Brichta Alan M, Tooney Paul A, Beveridge Natalie J, Graham Brett A, Anderson Wayne B, and Callister Robert J

Subjects
Pathology, RB1-214
Abstract

Abstract Background Neurons in superficial (SDH) and deep (DDH) laminae of the spinal cord dorsal horn receive sensory information from skin, muscle, joints and viscera. In both regions, glycine- (GlyR) and GABAA-receptors (GABAARs) contribute to fast synaptic inhibition. For rat, several types of GABAAR coexist in the two regions and each receptor type provides different contributions to inhibitory tone. Recent work in mouse has discovered an additional type of GlyR, (containing alpha 3 subunits) in the SDH. The contribution of differing forms of the GlyR to sensory processing in SDH and DDH is not understood. Methods and Results Here we compare fast inhibitory synaptic transmission in mouse (P17-37) SDH and DDH using patch-clamp electrophysiology in transverse spinal cord slices (L3-L5 segments, 23°C). GlyR-mediated mIPSCs were detected in 74% (25/34) and 94% (25/27) of SDH and DDH neurons, respectively. In contrast, GABAAR-mediated mIPSCs were detected in virtually all neurons in both regions (93%, 14/15 and 100%, 18/18). Several Gly- and GABAAR properties also differed in SDH vs. DDH. GlyR-mediated mIPSC amplitude was smaller (37.1 ± 3.9 vs. 64.7 ± 5.0 pA; n = 25 each), decay time was slower (8.5 ± 0.8 vs. 5.5 ± 0.3 ms), and frequency was lower (0.15 ± 0.03 vs. 0.72 ± 0.13 Hz) in SDH vs. DDH neurons. In contrast, GABAAR-mediated mIPSCs had similar amplitudes (25.6 ± 2.4, n = 14 vs. 25. ± 2.0 pA, n = 18) and frequencies (0.21 ± 0.08 vs. 0.18 ± 0.04 Hz) in both regions; however, decay times were slower (23.0 ± 3.2 vs. 18.9 ± 1.8 ms) in SDH neurons. Mean single channel conductance underlying mIPSCs was identical for GlyRs (54.3 ± 1.6 pS, n = 11 vs. 55.7 ± 1.8, n = 8) and GABAARs (22.7 ± 1.7 pS, n = 10 vs. 22.4 ± 2.0 pS, n = 11) in both regions. We also tested whether the synthetic endocanabinoid, methandamide (methAEA), had direct effects on Gly- and GABAARs in each spinal cord region. MethAEA (5 μM) reduced GlyR-mediated mIPSC frequency in SDH and DDH, but did not affect other properties. Similar results were observed for GABAAR mediated mIPSCs, however, rise time was slowed by methAEA in SDH neurons. Conclusion Together these data show that Gly- and GABAARs with clearly differing physiological properties and cannabinoid-sensitivity contribute to fast synaptic inhibition in mouse SDH and DDH.

Published
2009
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37. Anatomical and Molecular Properties of Long Descending Propriospinal Neurons in Mice

Author

Flynn, Jamie R., Conn, Victoria, Boyle, Kieran, Hughes, David I., Watanabe, Masahiko, Velasquez, Tomoko, Goulding, Martyn D., Callister, Robert J., and Graham, Brett A.

Subjects
propriospinal, neuroanatomy, synapse, morphology, inhibitory, developmental genetics, Original Research, Neuroscience
Abstract

Long descending propriospinal neurons (LDPNs) are interneurons that form direct connections between cervical and lumbar spinal circuits. LDPNs are involved in interlimb coordination and are important mediators of functional recovery after spinal cord injury (SCI). Much of what we know about LDPNs comes from a range of species, however, the increased use of transgenic mouse lines to better define neuronal populations calls for a more complete characterisation of LDPNs in mice. In this study, we examined the cell body location, inhibitory neurotransmitter phenotype, developmental provenance, morphology and synaptic inputs of mouse LDPNs throughout the cervical and upper thoracic spinal cord. LDPNs were retrogradely labelled from the lumbar spinal cord to map cell body locations throughout the cervical and upper thoracic segments. Ipsilateral LDPNs were distributed throughout the dorsal, intermediate and ventral grey matter as well as the lateral spinal nucleus and lateral cervical nucleus. In contrast, contralateral LDPNs were more densely concentrated in the ventromedial grey matter. Retrograde labelling in GlyT2GFP and GAD67GFP mice showed the majority of inhibitory LDPNs project either ipsilaterally or adjacent to the midline. Additionally, we used several transgenic mouse lines to define the developmental provenance of LDPNs and found that V2b positive neurons form a subset of ipsilaterally projecting LDPNs. Finally, a population of Neurobiotin (NB) labelled LDPNs were assessed in detail to examine morphology and plot the spatial distribution of contacts from a variety of neurochemically distinct axon terminals. These results provide important baseline data in mice for future work on their role in locomotion and recovery from SCI.

Published
2017
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41. Electrical maturation of spinal neurons in the human fetus: comparison of ventral and dorsal horn

Author

Tadros, Melissa Anne, Lim, Rebecca, Hughes, David I., Brichta, Alan Martin, and Callister, Robert J.

Abstract

The spinal cord is critical for modifying and relaying sensory information to, and motor commands from, higher centres in the central nervous system to initiate and maintain contextually relevant locomotor responses. Our understanding of how spinal sensorimotor circuits are established during in utero development is based largely on studies in rodents. In contrast, there is little functional data on the development of sensory and motor systems in humans. Here, we use patch clamp electrophysiology to examine the development of neuronal excitability in human fetal spinal cords (10-18 weeks gestation; WG). Transverse spinal cord slices (300 µm thick) were prepared and recordings were made from visualized neurons in either the ventral (VH) or dorsal (DH) horn at 32oC. Action potentials (APs) could be elicited in VH neurons throughout the period examined, but only after 16 WG in DH neurons. At this age, VH neurons discharged multiple APs, whereas most DH neurons discharged single APs. In addition, at 16-18 WG VH neurons also displayed larger AP and after-hyperpolarization amplitudes than DH neurons. Between 10 and 18 WG the intrinsic properties of VH neurons changed markedly, with input resistance decreasing and AP and AHP amplitudes increasing. These findings are consistent with the hypothesis that VH motor circuitry matures more rapidly than the DH circuits that are involved in processing tactile and nociceptive information.

Published
2015

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