43 results on '"Callaway, JK"'
Search Results
2. Memory Impairment in Rats after Desflurane Anesthesia is Age and Dose Dependent
- Author
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Callaway, JK, Jones, NC, Royse, AG, Royse, CF, Callaway, JK, Jones, NC, Royse, AG, and Royse, CF
- Abstract
Post-operative cognitive dysfunction (POCD) predominantly affects the elderly who suffer memory and concentration deficits after anesthesia and surgery. Animal studies have demonstrated anesthetic alone may contribute to POCD but results are variable and little is known about common anesthetics other than isoflurane. The present study investigated dose-dependence of desflurane anesthesia in young adult and aged rats. We hypothesize higher concentrations of desflurane will result in memory impairment in the water maze and that impairment will be worse in aged rats. Effects of anesthesia (1 or 1.5 MAC, 4 h) desflurane, or sham exposure on cognition were investigated in young adult (3 months) and aged (20-24 months) rats at 1, 4, and 12 weeks post-exposure. The Morris water maze was used to assess acquisition and retention of spatial reference memory. Latency to find the hidden platform and swimming speed were compared between treatments. Aged rats showed significant impairment in task acquisition after exposure to 1.5 MAC, but not 1.0 MAC desflurane anesthetic when tested 1 week following exposure. Latency to find the platform and distance travelled were significantly longer in aged rats given 1.5 MAC desflurane (latency: F(1,108) = 19.71, p < 0.0001; distance: F(1,108) = 5.79, p = 0.018). Deficits were not long-lasting and were no longer present at 4 or 12 weeks. In contrast, young adult rats performed equally as well as sham-exposed control rats irrespective of desflurane dose. This study showed the effects of desflurane on learning and memory in the water maze are age and dose dependent and are brief in duration.
- Published
- 2015
3. Direct Angiotensin AT2 Receptor Stimulation Using a Novel AT2 Receptor Agonist, Compound 21, Evokes Neuroprotection in Conscious Hypertensive Rats
- Author
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Karamyan, V, McCarthy, CA, Vinh, A, Miller, AA, Hallberg, A, Alterman, M, Callaway, JK, Widdop, RE, Karamyan, V, McCarthy, CA, Vinh, A, Miller, AA, Hallberg, A, Alterman, M, Callaway, JK, and Widdop, RE
- Abstract
BACKGROUND: In this study, the neuroprotective effect of a novel nonpeptide AT2R agonist, C21, was examined in a conscious model of stroke to verify a class effect of AT2R agonists as neuroprotective agents. METHODS AND RESULTS: Spontaneously hypertensive rats (SHR) were pre-treated for 5 days prior to stroke with C21 alone or in combination with the AT2R antagonist PD123319. In a separate series of experiments C21 was administered in a series of 4 doses commencing 6 hours after stroke. A focal reperfusion model of ischemia was induced in conscious SHR by administering endothelin-1 to the middle cerebral artery (MCA). Motor coordination was assessed at 1 and 3 days after stroke and post mortem analyses of infarct volumes, microglia activation and neuronal survival were performed at 72 hours post MCA occlusion. When given prior to stroke, C21 dose dependently decreased infarct volume, which is consistent with the behavioural findings illustrating an improvement in motor deficit. During the pre-treatment protocol C21 was shown to enhance microglia activation, which are likely to be evoking protection by releasing brain derived neurotrophic factor. When drug administration was delayed until 6 hours after stroke, C21 still reduced brain injury. CONCLUSION: These results indicate that centrally administered C21 confers neuroprotection against stroke damage. This benefit is likely to involve various mechanisms, including microglial activation of endogenous repair and enhanced cerebroperfusion. Thus, we have confirmed the neuroprotective effect of AT2R stimulation using a nonpeptide compound which highlights the clinical potential of the AT2R agonists for future development.
- Published
- 2014
4. Reply to: Isoflurane is not necessarily the only cause of cognitive deficits
- Author
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Royse, CF, Callaway, JK, Jones, NC, Royse, CF, Callaway, JK, and Jones, NC
- Abstract
Editor, We thank Braunecker and Hinkelbein1 for their interest in our recent manuscript.2 We made no claim that our findings with isoflurane anaesthetic in young adult and middle-aged rats were completely novel. Braunecker and Hinkelbein1 are correct in stating that previous studies have indeed reported enduring effects of isoflurane and other anaesthetic gases on the brain. However, the studies to which Braunecker and Hinkelbein refer have not investigated any physiological or behavioural consequences of anaesthetic-induced protein changes in the brain. We are primarily concerned with postoperative cognitive dysfunction and whether exposure to anaesthetics may be a causative factor. From this vantage point relating changes in the brain to the observed deficit is the secondary outcome measure. Certainly, the protein changes observed by authors such as Kalenka et al. 3,4 may provide clues to the mechanism of action of isoflurane and other volatile anaesthetic agents on cognition. We did not expose our sham rats to 100% oxygen for the full 4 h and we do not believe this carrier gas is responsible for the observed deficit. Other researchers have reported deficits in cognition after exposure to isoflurane but have not employed 100% oxygen as the carrier. Isoflurane (1.2%) in 30% oxygen and 70% nitrous oxide impaired performance in the radial arm maze in both adult and aged rats.5,6 A recent study in mice found low concentrations of isoflurane (1%) delivered in 100% oxygen impaired, whereas high concentrations (1.5 and 2%) improved learning and memory in the Morris water maze.7 Given that there are diametrically opposed outcomes reported following isoflurane exposure, future studies must surely include cognitive outcome in conjunction with brain analyses. The protein changes detected after isoflurane and/or 100% oxygen exposure are without significance until they can be related in some way to a physiological response.
- Published
- 2013
5. Ndfip1 regulates nuclear Pten import in vivo to promote neuronal survival following cerebral ischemia
- Author
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Howitt, J, Lackovic, J, Low, L-H, Naguib, A, Macintyre, A, Goh, C-P, Callaway, JK, Hammond, V, Thomas, T, Dixon, M, Putz, U, Silke, J, Bartlett, P, Yang, B, Kumar, S, Trotman, LC, Tan, S-S, Howitt, J, Lackovic, J, Low, L-H, Naguib, A, Macintyre, A, Goh, C-P, Callaway, JK, Hammond, V, Thomas, T, Dixon, M, Putz, U, Silke, J, Bartlett, P, Yang, B, Kumar, S, Trotman, LC, and Tan, S-S
- Abstract
PTEN (phosphatase and tensin homologue deleted on chromosome TEN) is the major negative regulator of phosphatidylinositol 3-kinase signaling and has cell-specific functions including tumor suppression. Nuclear localization of PTEN is vital for tumor suppression; however, outside of cancer, the molecular and physiological events driving PTEN nuclear entry are unknown. In this paper, we demonstrate that cytoplasmic Pten was translocated into the nuclei of neurons after cerebral ischemia in mice. Critically, this transport event was dependent on a surge in the Nedd4 family-interacting protein 1 (Ndfip1), as neurons in Ndfip1-deficient mice failed to import Pten. Ndfip1 binds to Pten, resulting in enhanced ubiquitination by Nedd4 E3 ubiquitin ligases. In vitro, Ndfip1 overexpression increased the rate of Pten nuclear import detected by photobleaching experiments, whereas Ndfip1(-/-) fibroblasts showed negligible transport rates. In vivo, Ndfip1 mutant mice suffered larger infarct sizes associated with suppressed phosphorylated Akt activation. Our findings provide the first physiological example of when and why transient shuttling of nuclear Pten occurs and how this process is critical for neuron survival.
- Published
- 2012
6. Spontaneous Development of Full Weight-Supported Stepping after Complete Spinal Cord Transection in the Neonatal Opossum, Monodelphis domestica
- Author
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Fenton, B, Wheaton, BJ, Callaway, JK, Ek, CJ, Dziegielewska, KM, Saunders, NR, Fenton, B, Wheaton, BJ, Callaway, JK, Ek, CJ, Dziegielewska, KM, and Saunders, NR
- Abstract
Spinal cord trauma in the adult nervous system usually results in permanent loss of function below the injury level. The immature spinal cord has greater capacity for repair and can develop considerable functionality by adulthood. This study used the marsupial laboratory opossum Monodelphis domestica, which is born at a very early stage of neural development. Complete spinal cord transection was made in the lower-thoracic region of pups at postnatal-day 7 (P7) or P28, and the animals grew to adulthood. Injury at P7 resulted in a dense neuronal tissue bridge that connected the two ends of the cord; retrograde neuronal labelling indicated that supraspinal and propriospinal innervation spanned the injury site. This repair was associated with pronounced behavioural recovery, coordinated gait and an ability to use hindlimbs when swimming. Injury at P28 resulted in a cyst-like cavity encased in scar tissue forming at the injury site. Using retrograde labelling, no labelled brainstem or propriospinal neurons were found above the lesion, indicating that detectable neuronal connectivity had not spanned the injury site. However, these animals could use their hindlimbs to take weight-supporting steps but could not use their hindlimbs when swimming. White matter, demonstrated by Luxol Fast Blue staining, was present in the injury site of P7- but not P28-injured animals. Overall, these studies demonstrated that provided spinal injury occurs early in development, regrowth of supraspinal innervation is possible. This repair appears to lead to improved functional outcomes. At older ages, even without detectable axonal growth spanning the injury site, substantial development of locomotion was still possible. This outcome is discussed in conjunction with preliminary findings of differences in the local propriospinal circuits following spinal cord injury (demonstrated with fluororuby labelling), which may underlie the weight bearing locomotion observed in the apparent absence of axons
- Published
- 2011
7. Spatio-Temporal Progression of Grey and White Matter Damage Following Contusion Injury in Rat Spinal Cord
- Author
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Combs, C, Ek, CJ, Habgood, MD, Callaway, JK, Dennis, R, Dziegielewska, KM, Johansson, PA, Potter, A, Wheaton, B, Saunders, NR, Combs, C, Ek, CJ, Habgood, MD, Callaway, JK, Dennis, R, Dziegielewska, KM, Johansson, PA, Potter, A, Wheaton, B, and Saunders, NR
- Abstract
Cellular mechanisms of secondary damage progression following spinal cord injury remain unclear. We have studied the extent of tissue damage from 15 min to 10 weeks after injury using morphological and biochemical estimates of lesion volume and surviving grey and white matter. This has been achieved by semi-quantitative immunocytochemical methods for a range of cellular markers, quantitative counts of white matter axonal profiles in semi-thin sections and semi-quantitative Western blot analysis, together with behavioural tests (BBB scores, ledged beam, random rung horizontal ladder and DigiGait analysis). We have developed a new computer-controlled electronic impactor based on a linear motor that allows specification of the precise nature, extent and timing of the impact. Initial (15 min) lesion volumes showed very low variance (1.92+/-0.23 mm3, mean+/-SD, n=5). Although substantial tissue clearance continued for weeks after injury, loss of grey matter was rapid and complete by 24 hours, whereas loss of white matter extended up to one week. No change was found between one and 10 weeks after injury for almost all morphological and biochemical estimates of lesion size or behavioural methods. These results suggest that previously reported apparent ongoing injury progression is likely to be due, to a large extent, to clearance of tissue damaged by the primary impact rather than continuing cell death. The low variance of the impactor and the comprehensive assessment methods described in this paper provide an improved basis on which the effects of potential treatment regimes for spinal cord injury can be assessed.
- Published
- 2010
8. Sevoflurane anesthesia does not impair acquisition learning or memory in the morris water maze in young adult and aged rats.
- Author
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Callaway JK, Jones NC, Royse AG, and Royse CF
- Abstract
BACKGROUND: Sevoflurane has been found to increase apoptosis and pathologic markers associated with Alzheimer disease, provoking concern over their potential contribution to postoperative cognitive dysfunction. METHODS: The effects of anesthesia with 1 minimum alveolar concentration of sevoflurane for 4 h or sham exposure on cognition were investigated in young adult and aged (20-24 months) rats at 1, 4, and 12 weeks postexposure. Spatial reference memory acquisition and retention were tested in the Morris water maze task. Latency to locate the hidden platform and swim speed were determined and compared between treatments. RESULTS: Sevoflurane anesthesia significantly reduced latency to find the hidden platform in both young adult (n = 10 per treatment, P < 0.0001) and aged rats (n = 7 per treatment, P < 0.0001) when tested 1 week after exposure. In young rats only, this improved acquisition learning was maintained at 4 (P = 0.003) but not at 12 weeks postexposure (P = 0.061). There were no differences in swim speed or in open field exploration between groups (no confounding effects of stress or locomotion). Retention memory measured using probe trials was not affected by exposure to sevoflurane in young adult or aged rats. CONCLUSION: Sevoflurane anesthesia did not impair acquisition learning and retention memory in young adult or aged rats. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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9. Angiotensin AT2 receptor stimulation causes neuroprotection in a conscious rat model of stroke.
- Author
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McCarthy CA, Vinh A, Callaway JK, Widdop RE, McCarthy, Claudia A, Vinh, Antony, Callaway, Jennifer K, and Widdop, Robert E
- Published
- 2009
- Full Text
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10. Reply to: Isoflurane is not necessarily the only cause of cognitive deficits.
- Author
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Callaway JK, Jones NC, and Royse CF
- Published
- 2013
- Full Text
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11. Longitudinal outcomes of radiofrequency ablation versus surveillance endoscopy for Barrett's esophagus with low-grade dysplasia.
- Author
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Kahn A, Al-Qaisi M, Kommineni VT, Callaway JK, Boroff ES, Burdick GE, Lam-Himlin DM, Temkit M, Vela MF, and Ramirez FC
- Subjects
- Adenocarcinoma pathology, Aged, Barrett Esophagus pathology, Disease Progression, Esophageal Neoplasms pathology, Esophagus surgery, Female, Humans, Hyperplasia surgery, Longitudinal Studies, Male, Middle Aged, Precancerous Conditions pathology, Proportional Hazards Models, Retrospective Studies, Risk Factors, Time Factors, Treatment Outcome, Adenocarcinoma surgery, Barrett Esophagus surgery, Catheter Ablation statistics & numerical data, Esophageal Neoplasms surgery, Esophagoscopy statistics & numerical data, Esophagus pathology, Precancerous Conditions surgery
- Abstract
Radiofrequency ablation of Barrett's esophagus with low-grade dysplasia is recommended in recent American College of Gastroenterology guidelines, with endoscopic surveillance considered a reasonable alternative. Few studies have directly compared outcomes of radiofrequency ablation to surveillance and those that have are limited by short duration of follow-up. This study aims to compare the long-term effectiveness of radiofrequency ablation versus endoscopic surveillance in a large, longitudinal cohort of patients with Barrett's esophagus, and low-grade dysplasia.We conducted a retrospective analysis of patients with confirmed low-grade dysplasia at a single academic medical center from 1991 to 2014. Patients progressing to high-grade dysplasia or esophageal adenocarcinoma within one year of index LGD endoscopy were defined as missed dysplasia and excluded. Risk factors for progression were assessed via Cox proportional hazards model. Comparison of progression risk was conducted using a Kaplan-Meier analysis. Subset analyses were conducted to examine the effect of reintroducing early progressors and excluding patients diagnosed prior to the advent of ablative therapy. Of 173 total patients, 79 (45.7%) underwent radiofrequency ablation while 94 (54.3%) were untreated, with median follow up of 90 months. Seven (8.9%) patients progressed to high-grade dysplasia or adenocarcinoma despite ablation, compared with 14 (14.9%) undergoing surveillance (P = 0.44). This effect was preserved when patients diagnosed prior to the introduction of radiofrequency ablation were excluded (8.9% vs 13%, P = 0.68). Reintroduction of patients progressing within the first year of follow-up resulted in a trend toward significance for ablation versus surveillance (11.1% vs 23.8%, P = 0.053).In conclusion, progression to high-grade dysplasia or adenocarcinoma was not significantly reduced in the radiofrequency ablation cohort when compared to surveillance. Despite recent studies suggesting the superiority of radiofrequency ablation in reducing progression, diligent endoscopic surveillance may provide similar long-term outcomes.
- Published
- 2018
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12. Isoflurane in the presence or absence of surgery increases hippocampal cytokines associated with memory deficits and responses to brain injury in rats.
- Author
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Callaway JK, Wood C, Jenkins TA, Royse AG, and Royse CF
- Subjects
- Animals, Conditioning, Classical drug effects, Fear drug effects, Hippocampus metabolism, Male, Memory Disorders metabolism, Rats, Rats, Sprague-Dawley, Cytokines metabolism, Hippocampus drug effects, Isoflurane adverse effects, Laparotomy adverse effects, Memory Disorders chemically induced
- Abstract
Evidence from experimental animal studies convincingly argues for a role of pro-inflammatory cytokines due to surgical trauma in causing postoperative cognitive dysfunction. However, other studies have shown exposure to 2-4h of isoflurane anesthetic without surgical trauma can also impair cognitive function. We aimed to determine cytokine changes over time following isoflurane exposure in the presence and absence of surgery and examine subsequent cognitive function. Male rats were exposed to isoflurane (1.8%, 4h) with or without laparotomy or control conditions and tested in a contextual fear conditioning paradigm 8 days later. On day 9 rats were perfused, serum and hippocampal samples were collected and 24 cytokines were analysed. Groups of rats exposed as above were killed 6 or 48h after isoflurane exposure to examine early cytokine changes. Isoflurane exposure resulted in significantly less freezing in the contextual fear conditioning test (F(2,31)=6.11, P=0.006) and addition of laparotomy caused no further deficits (P>0.05). At 6h post isoflurane exposure an immunosuppressive response was observed in the serum while hippocampal cytokines were largely unchanged. These finding suggest isoflurane alone causes inflammatory changes and cognitive deficits. The addition of a laparotomy had a negligible effect. Early after isoflurane exposure changes in serum and hippocampal cytokines were divergent but by 9 days were aligned. At this time cytokines associated with memory deficits and brain injury processes were significantly elevated in serum and brain., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
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13. Memory impairment in rats after desflurane anesthesia is age and dose dependent.
- Author
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Callaway JK, Jones NC, Royse AG, and Royse CF
- Subjects
- Age Factors, Animals, Desflurane, Dose-Response Relationship, Drug, Isoflurane adverse effects, Male, Maze Learning drug effects, Rats, Rats, Sprague-Dawley, Time Factors, Aging, Anesthetics, Inhalation adverse effects, Isoflurane analogs & derivatives, Memory Disorders chemically induced
- Abstract
Post-operative cognitive dysfunction (POCD) predominantly affects the elderly who suffer memory and concentration deficits after anesthesia and surgery. Animal studies have demonstrated anesthetic alone may contribute to POCD but results are variable and little is known about common anesthetics other than isoflurane. The present study investigated dose-dependence of desflurane anesthesia in young adult and aged rats. We hypothesize higher concentrations of desflurane will result in memory impairment in the water maze and that impairment will be worse in aged rats. Effects of anesthesia (1 or 1.5 MAC, 4 h) desflurane, or sham exposure on cognition were investigated in young adult (3 months) and aged (20-24 months) rats at 1, 4, and 12 weeks post-exposure. The Morris water maze was used to assess acquisition and retention of spatial reference memory. Latency to find the hidden platform and swimming speed were compared between treatments. Aged rats showed significant impairment in task acquisition after exposure to 1.5 MAC, but not 1.0 MAC desflurane anesthetic when tested 1 week following exposure. Latency to find the platform and distance travelled were significantly longer in aged rats given 1.5 MAC desflurane (latency: F(1,108) = 19.71, p < 0.0001; distance: F(1,108) = 5.79, p = 0.018). Deficits were not long-lasting and were no longer present at 4 or 12 weeks. In contrast, young adult rats performed equally as well as sham-exposed control rats irrespective of desflurane dose. This study showed the effects of desflurane on learning and memory in the water maze are age and dose dependent and are brief in duration.
- Published
- 2015
- Full Text
- View/download PDF
14. Direct angiotensin AT2 receptor stimulation using a novel AT2 receptor agonist, compound 21, evokes neuroprotection in conscious hypertensive rats.
- Author
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McCarthy CA, Vinh A, Miller AA, Hallberg A, Alterman M, Callaway JK, and Widdop RE
- Subjects
- Animals, Endothelin-1 pharmacology, Male, Rats, Reperfusion Injury chemically induced, Reperfusion Injury drug therapy, Hypertension drug therapy, Neuroprotective Agents therapeutic use, Receptor, Angiotensin, Type 2 agonists, Receptor, Angiotensin, Type 2 metabolism
- Abstract
Background: In this study, the neuroprotective effect of a novel nonpeptide AT2R agonist, C21, was examined in a conscious model of stroke to verify a class effect of AT2R agonists as neuroprotective agents., Methods and Results: Spontaneously hypertensive rats (SHR) were pre-treated for 5 days prior to stroke with C21 alone or in combination with the AT2R antagonist PD123319. In a separate series of experiments C21 was administered in a series of 4 doses commencing 6 hours after stroke. A focal reperfusion model of ischemia was induced in conscious SHR by administering endothelin-1 to the middle cerebral artery (MCA). Motor coordination was assessed at 1 and 3 days after stroke and post mortem analyses of infarct volumes, microglia activation and neuronal survival were performed at 72 hours post MCA occlusion. When given prior to stroke, C21 dose dependently decreased infarct volume, which is consistent with the behavioural findings illustrating an improvement in motor deficit. During the pre-treatment protocol C21 was shown to enhance microglia activation, which are likely to be evoking protection by releasing brain derived neurotrophic factor. When drug administration was delayed until 6 hours after stroke, C21 still reduced brain injury., Conclusion: These results indicate that centrally administered C21 confers neuroprotection against stroke damage. This benefit is likely to involve various mechanisms, including microglial activation of endogenous repair and enhanced cerebroperfusion. Thus, we have confirmed the neuroprotective effect of AT2R stimulation using a nonpeptide compound which highlights the clinical potential of the AT2R agonists for future development.
- Published
- 2014
- Full Text
- View/download PDF
15. Angiotensin II type 2 receptor stimulation initiated after stroke causes neuroprotection in conscious rats.
- Author
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McCarthy CA, Vinh A, Broughton BR, Sobey CG, Callaway JK, and Widdop RE
- Subjects
- Animals, Apoptosis drug effects, Blood Pressure drug effects, Cell Survival drug effects, Male, Motor Activity drug effects, Neuroprotective Agents pharmacology, Oligopeptides pharmacology, Rats, Rats, Inbred SHR, Recovery of Function drug effects, Brain Ischemia drug therapy, Neuroprotective Agents therapeutic use, Oligopeptides therapeutic use, Receptor, Angiotensin, Type 2 agonists, Stroke drug therapy
- Abstract
We have demonstrated previously that pretreatment with an angiotensin II type 2 receptor (AT(2)R) agonist is neuroprotective against a subsequent stroke independent of any changes in blood pressure. Therefore, in the current study, we have examined the potential neuroprotective effect of AT(2)R stimulation initiated after stroke induction to mimic the clinical setting. Intracerebroventricular administration of the AT(2)R agonist CGP42112 was commenced 6 hours after an ischemic stroke had been induced in conscious spontaneously hypertensive rats. CGP42112 given over 4 doses in the same rats (3 µg/kg per dose centrally) at 6, 24, 48, and 72 hours after stroke induction reduced total infarct volume (32 ± 13 mm(3) versus vehicle, 170 ± 49 mm(3); P<0.05) and improved motor function. Furthermore, we have demonstrated that AT(2)R stimulation after stroke increased neuronal survival, decreased apoptosis, and caused an increase in the number of activated microglia in the core region of damage. The effects of CGP42112 were partially reversed with the coadministration of an AT(2)R antagonist, PD123319. Thus, the current study has shown for the first time that delayed central AT(2)R stimulation after a cerebral incident is neuroprotective in a conscious rat model of stroke.
- Published
- 2012
- Full Text
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16. Differential regulation of Nedd4 ubiquitin ligases and their adaptor protein Ndfip1 in a rat model of ischemic stroke.
- Author
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Lackovic J, Howitt J, Callaway JK, Silke J, Bartlett P, and Tan SS
- Subjects
- Animals, Apoptosis physiology, Brain Ischemia genetics, Carrier Proteins genetics, Endosomal Sorting Complexes Required for Transport genetics, Male, Membrane Proteins genetics, Nedd4 Ubiquitin Protein Ligases, Rats, Rats, Wistar, Stroke genetics, Ubiquitin-Protein Ligases genetics, Up-Regulation, Brain Ischemia metabolism, Carrier Proteins metabolism, Cerebral Cortex metabolism, Endosomal Sorting Complexes Required for Transport metabolism, Membrane Proteins metabolism, Neurons metabolism, Stroke metabolism, Ubiquitin-Protein Ligases metabolism
- Abstract
Ubiquitin-modification of proteins by E3 ubiquitin ligases is an important post-translational mechanism implicated in neuronal survival and injury following cerebral ischemia. However, of the 500 or so E3s thought to be present in mammalian cells, very few specific E3s have been identified and associated with brain ischemia. Here, we demonstrate endogenous induction of HECT-type E3 ligases of the Nedd4 family and their adaptor Nedd4-family interacting protein 1 (Ndfip1) following transient focal cerebral ischemia in rats. Ndfip1 is upregulated in surviving cortical neurons and its neuroprotective activity is correlated with Nedd4-2 upregulation, but not two other Nedd4 family members examined (Nedd4-1 and Itch). Immunoprecipitation assays confirmed biochemical binding of Ndfip1 with Nedd4-2 in the brain, with or without ischemic stroke, indicating their endogenous interaction. While Ndfip1 and Itch have been previously shown to interact outside of the nervous system, ischemic induction of Itch in the present study was associated with cellular survival independent of Ndfip1. Together, these findings demonstrate specific and differential regulation of Nedd4 family E3 ligases under ischemic conditions, and identify two E3 ligases and their adaptor that potentially regulate ubiquitination in ischemic stroke to provide neuroprotection., (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
17. Isoflurane induces cognitive deficits in the Morris water maze task in rats.
- Author
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Callaway JK, Jones NC, and Royse CF
- Subjects
- Age Factors, Animals, Cognition Disorders physiopathology, Cognition Disorders psychology, Learning drug effects, Male, Memory drug effects, Motor Activity drug effects, Rats, Rats, Sprague-Dawley, Reaction Time, Anesthetics, Inhalation toxicity, Behavior, Animal drug effects, Cognition drug effects, Cognition Disorders chemically induced, Isoflurane toxicity
- Abstract
Background: Postoperative cognitive dysfunction has been reported in young, middle-aged and elderly patients with greater incidence with increasing age. Neurocognitive deficits are associated with anaesthetic exposure in aged rodents but in younger adult animals the findings are inconsistent and in middle-aged animals they are unknown. We aimed to compare the effects of moderate duration isoflurane anaesthesia in 100% oxygen on Morris water maze performance in young adult and middle-aged rats. We hypothesised that isoflurane would have greater effects on learning and memory in middle-aged compared with young rats., Materials and Methods: Young adult (3 months, n = 25) and middle-aged (12 months, n = 20) male Sprague Dawley rats were assigned randomly to isoflurane exposure (1 minimum alveolar concentration, 4 h) or control conditions. Spatial learning (acquisition phase) and memory (probe trial) were tested in the Morris water maze 1 week after exposure. Middle-aged rats were retested in the probe trial 4 weeks after exposure for long-term memory retention. Latency to locate the hidden platform and time spent in the platform quadrant were compared between ages and treatments., Results: Isoflurane did not affect acquisition of the water maze task in either age group. Isoflurane exposure induced a significant deficit in memory retention in young rats, but not middle-aged rats, in the probe trial 24 h after acquisition. Irrespective of treatment, middle-aged rats took longer to acquire the task than young rats. Four weeks after exposure, isoflurane-treated middle-aged rats showed no preference for target location, compared with sham-exposed rats which retained memory for previous platform location., Conclusion: Isoflurane exposure impaired retention memory for platform location 1 week after exposure in young adult rats and resulted in a delayed although weak impairment at 4 weeks in middle-aged rats. The great variation in reported effects of isoflurane suggests a complex effect of this agent on memory which warrants further investigation.
- Published
- 2012
- Full Text
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18. Ndfip1 regulates nuclear Pten import in vivo to promote neuronal survival following cerebral ischemia.
- Author
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Howitt J, Lackovic J, Low LH, Naguib A, Macintyre A, Goh CP, Callaway JK, Hammond V, Thomas T, Dixon M, Putz U, Silke J, Bartlett P, Yang B, Kumar S, Trotman LC, and Tan SS
- Subjects
- Animals, Brain Ischemia pathology, Carrier Proteins genetics, Carrier Proteins metabolism, Cell Survival, Endosomal Sorting Complexes Required for Transport physiology, Intercellular Signaling Peptides and Proteins, Membrane Proteins genetics, Membrane Proteins metabolism, Mice, Mice, Inbred C57BL, Nedd4 Ubiquitin Protein Ligases, Photobleaching, Protein Transport, Ubiquitin-Protein Ligases physiology, Ubiquitination, Brain Ischemia metabolism, Carrier Proteins physiology, Membrane Proteins physiology, Neurons physiology, PTEN Phosphohydrolase metabolism
- Abstract
PTEN (phosphatase and tensin homologue deleted on chromosome TEN) is the major negative regulator of phosphatidylinositol 3-kinase signaling and has cell-specific functions including tumor suppression. Nuclear localization of PTEN is vital for tumor suppression; however, outside of cancer, the molecular and physiological events driving PTEN nuclear entry are unknown. In this paper, we demonstrate that cytoplasmic Pten was translocated into the nuclei of neurons after cerebral ischemia in mice. Critically, this transport event was dependent on a surge in the Nedd4 family-interacting protein 1 (Ndfip1), as neurons in Ndfip1-deficient mice failed to import Pten. Ndfip1 binds to Pten, resulting in enhanced ubiquitination by Nedd4 E3 ubiquitin ligases. In vitro, Ndfip1 overexpression increased the rate of Pten nuclear import detected by photobleaching experiments, whereas Ndfip1(-/-) fibroblasts showed negligible transport rates. In vivo, Ndfip1 mutant mice suffered larger infarct sizes associated with suppressed phosphorylated Akt activation. Our findings provide the first physiological example of when and why transient shuttling of nuclear Pten occurs and how this process is critical for neuron survival.
- Published
- 2012
- Full Text
- View/download PDF
19. Potent inhibition of anoxic depolarization by the sodium channel blocker dibucaine.
- Author
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Douglas HA, Callaway JK, Sword J, Kirov SA, and Andrew RD
- Subjects
- Action Potentials drug effects, Animals, Astrocytes pathology, Dendrites pathology, Dibucaine pharmacology, Enzyme Inhibitors pharmacology, Female, Hypoxia-Ischemia, Brain etiology, Male, Mice, Mice, Transgenic, Models, Animal, Neurons pathology, Ouabain pharmacology, Rats, Rats, Sprague-Dawley, Sodium Channel Blockers pharmacology, Sodium-Potassium-Exchanging ATPase antagonists & inhibitors, Dibucaine therapeutic use, Hypoxia-Ischemia, Brain pathology, Hypoxia-Ischemia, Brain prevention & control, Sodium Channel Blockers therapeutic use, Stroke complications
- Abstract
Recurring waves of peri-infarct depolarizations (PIDs) propagate across gray matter in the hours and days following stroke, expanding the primary site of injury. Ischemic depolarization (termed anoxic depolarization or AD in live brain slices) is PID-like but immediately arises in the more metabolically compromised ischemic core. This causes dramatic neuronal and astrocyte swelling and dendritic beading with spine loss within minutes, resulting in acute cell death. AD is evoked in rodent neocortical slices by suppressing the Na(+)/K(+)-ATPase pump with either oxygen/glucose deprivation (OGD) or exposure to ouabain. The process driving AD and PIDs remains poorly understood. Here we show that dibucaine is a potent drug inhibiting AD because of its high binding affinity to the Na(+) channel. Field recording reveals that, when superfused with ouabain (5 min), neocortical slices pretreated with 1 μM dibucaine for 45 min display either no AD or delayed AD onset compared with untreated controls. If ouabain exposure is extended to 10 min, 1 μM dibucaine is still able to delay AD onset by ∼ 60%. Likewise, it delays OGD-evoked AD onset by ∼ 54% but does not depress action potentials (APs) or evoked orthodromic field potentials. Increasing dibucaine to 10 μM inhibits AP firing, gradually putting the slice into a stasis that inhibits AD onset but also renders the slice functionally quiescent. Two-photon microscopy reveals that 10 μM dibucaine pretreatment prevents or helps reverse ouabain-induced structural neuronal damage. Although the therapeutic range of dibucaine is quite narrow, dibucaine-like drugs could prove therapeutically useful in inhibiting PIDs and their resultant neuronal damage.
- Published
- 2011
- Full Text
- View/download PDF
20. Spontaneous development of full weight-supported stepping after complete spinal cord transection in the neonatal opossum, Monodelphis domestica.
- Author
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Wheaton BJ, Callaway JK, Ek CJ, Dziegielewska KM, and Saunders NR
- Subjects
- Animals, Gait, Swimming, Animals, Newborn, Locomotion, Opossums physiology, Spinal Cord Injuries physiopathology
- Abstract
Spinal cord trauma in the adult nervous system usually results in permanent loss of function below the injury level. The immature spinal cord has greater capacity for repair and can develop considerable functionality by adulthood. This study used the marsupial laboratory opossum Monodelphis domestica, which is born at a very early stage of neural development. Complete spinal cord transection was made in the lower-thoracic region of pups at postnatal-day 7 (P7) or P28, and the animals grew to adulthood. Injury at P7 resulted in a dense neuronal tissue bridge that connected the two ends of the cord; retrograde neuronal labelling indicated that supraspinal and propriospinal innervation spanned the injury site. This repair was associated with pronounced behavioural recovery, coordinated gait and an ability to use hindlimbs when swimming. Injury at P28 resulted in a cyst-like cavity encased in scar tissue forming at the injury site. Using retrograde labelling, no labelled brainstem or propriospinal neurons were found above the lesion, indicating that detectable neuronal connectivity had not spanned the injury site. However, these animals could use their hindlimbs to take weight-supporting steps but could not use their hindlimbs when swimming. White matter, demonstrated by Luxol Fast Blue staining, was present in the injury site of P7- but not P28-injured animals. Overall, these studies demonstrated that provided spinal injury occurs early in development, regrowth of supraspinal innervation is possible. This repair appears to lead to improved functional outcomes. At older ages, even without detectable axonal growth spanning the injury site, substantial development of locomotion was still possible. This outcome is discussed in conjunction with preliminary findings of differences in the local propriospinal circuits following spinal cord injury (demonstrated with fluororuby labelling), which may underlie the weight bearing locomotion observed in the apparent absence of axons bridging the lesion site in P28-injured Monodelphis.
- Published
- 2011
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21. The σ 1 receptor agonist 4-PPBP elicits ERK1/2 phosphorylation in primary neurons: a possible mechanism of neuroprotective action.
- Author
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Tan F, Guio-Aguilar PL, Downes C, Zhang M, O'Donovan L, Callaway JK, and Crack PJ
- Subjects
- Analysis of Variance, Animals, Blotting, Western, Cell Survival drug effects, Cells, Cultured, Cerebral Cortex cytology, Cerebral Cortex drug effects, Cerebral Cortex metabolism, Dose-Response Relationship, Drug, Fluorescent Antibody Technique, Haloperidol pharmacology, Morpholines pharmacology, Neurons metabolism, Neuroprotective Agents pharmacology, Receptors, sigma agonists, Haloperidol analogs & derivatives, Mitogen-Activated Protein Kinase 1 metabolism, Mitogen-Activated Protein Kinase 3 metabolism, Neurons drug effects, Phosphorylation drug effects, Receptors, sigma metabolism
- Abstract
Although sigma 1 (σ(1)) receptors and mitogen-activated protein kinases (MAPKs) are known modulators of neuroprotection, a role for MAPK signaling pathways in σ receptor-mediated neuroprotection has not been investigated in detail.The present study aims to investigate the possible link between σ(1) receptors and MAPKs in neuroprotection. Primary mixed cortical and hippocampal neurons were treated with σ(1) receptor agonists PRE-084 or 4-PPBP in a time- and concentration-dependent manner; and in another set of experiments, cells were pre-incubated with σ(1) receptor antagonist BD1047 or MEK inhibitor PD98059 in a concentration-dependent manner prior to PRE-084 or 4-PPBP treatment. Levels of phosphorylated and total ERK1/2, JNK and p38-MAPK were determined with western blotting and ERK1/2 phosphorylation was confirmed with immunofluorescence. To investigate neuroprotection by σ(1) receptors, cells were pre-treated with PRE-084 or 4-PPBP and glucose-starved for various times: in the presence or absence of pre-incubated BD1047 or PD98059. Cell viability was then measured with MTT assay. Both PRE-084 and 4-PPBP caused phosphorylation of ERK1/2, but not p38-MAPK and JNK. ERK1/2 phosphorylation was inhibited by BD1047 and PD98059 in a concentration-dependent manner. Immunofluorescence confirmed the phosphorylation of ERK1/2 by PRE-084 and 4-PPBP and its inhibition by BD1047 and PD98059. Pre-treating glucose-deprived neurons with 4-PPBP, but not PRE-084; caused neuroprotection which was inhibited by BD1047 and PD98059. 4-PPBP, but not PRE-084; causes ERK1/2 phosphorylation-mediated neuroprotection. This presents a novel mechanism by σ(1) receptors in modulating neuroprotection., (Crown Copyright © 2010. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2010
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22. Spatio-temporal progression of grey and white matter damage following contusion injury in rat spinal cord.
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Ek CJ, Habgood MD, Callaway JK, Dennis R, Dziegielewska KM, Johansson PA, Potter A, Wheaton B, and Saunders NR
- Subjects
- Animals, Axons pathology, Behavior, Animal, Blotting, Western, Cell Count, Central Nervous System metabolism, Contusions metabolism, Disease Progression, Male, Nerve Fibers, Myelinated pathology, Neurons pathology, Rats, Rats, Sprague-Dawley, Spinal Cord Injuries metabolism, Time Factors, Central Nervous System pathology, Contusions pathology, Spinal Cord Injuries pathology
- Abstract
Cellular mechanisms of secondary damage progression following spinal cord injury remain unclear. We have studied the extent of tissue damage from 15 min to 10 weeks after injury using morphological and biochemical estimates of lesion volume and surviving grey and white matter. This has been achieved by semi-quantitative immunocytochemical methods for a range of cellular markers, quantitative counts of white matter axonal profiles in semi-thin sections and semi-quantitative Western blot analysis, together with behavioural tests (BBB scores, ledged beam, random rung horizontal ladder and DigiGait analysis). We have developed a new computer-controlled electronic impactor based on a linear motor that allows specification of the precise nature, extent and timing of the impact. Initial (15 min) lesion volumes showed very low variance (1.92+/-0.23 mm3, mean+/-SD, n=5). Although substantial tissue clearance continued for weeks after injury, loss of grey matter was rapid and complete by 24 hours, whereas loss of white matter extended up to one week. No change was found between one and 10 weeks after injury for almost all morphological and biochemical estimates of lesion size or behavioural methods. These results suggest that previously reported apparent ongoing injury progression is likely to be due, to a large extent, to clearance of tissue damaged by the primary impact rather than continuing cell death. The low variance of the impactor and the comprehensive assessment methods described in this paper provide an improved basis on which the effects of potential treatment regimes for spinal cord injury can be assessed.
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- 2010
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23. Long-term functional and protective actions of preconditioning with hypoxia, cobalt chloride, and desferrioxamine against hypoxic-ischemic injury in neonatal rats.
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Jones NM, Kardashyan L, Callaway JK, Lee EM, and Beart PM
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- Animals, Animals, Newborn, Behavior, Animal drug effects, Brain pathology, Brain physiopathology, Cobalt therapeutic use, Deferoxamine therapeutic use, Disease Models, Animal, Female, Hypoxia pathology, Hypoxia-Ischemia, Brain pathology, Hypoxia-Ischemia, Brain physiopathology, Male, Motor Skills drug effects, Neuroprotective Agents therapeutic use, Rats, Rats, Sprague-Dawley, Time Factors, Brain drug effects, Cobalt pharmacology, Deferoxamine pharmacology, Hypoxia physiopathology, Hypoxia-Ischemia, Brain prevention & control, Neuroprotective Agents pharmacology
- Abstract
Preconditioning with hypoxia and hypoxia-mimetic compounds cobalt chloride (CoCl2) and desferrioxamine (DFX) protects against hypoxic-ischemic (HI) injury in neonatal rat brain. We examined long-term functional and protective actions of preconditioning induced by hypoxia, CoCl(2) and DFX in a neonatal rat model of HI. Postnatal day six rat pups were exposed to preconditioning with hypoxia (8% oxygen) or injections of CoCl(2), DFX or saline vehicle and 24 h later rats underwent HI or sham surgery. Behavioral tests were performed and at the conclusion of experiments, brains removed for morphologic analyses. HI resulted in a large unilateral lesion in the ipsilateral hemisphere compared with sham control rats. All preconditioning treatments significantly reduced the total lesion volume. Behavioral deficits were observed in HI rats compared with sham controls. The reduction in forelimb grasping strength in HI rats was attenuated by preconditioning with hypoxia, CoCl(2) and DFX. HI increased the number of foot faults in a grid-walking test and resulted in forelimb asymmetry in the cylinder test. Only preconditioning with hypoxia reversed all three functional deficits after HI. These findings indicate that preconditioning, especially when induced by hypoxia, has the potential to minimize the morphologic and functional effects of neonatal HI injury.
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- 2008
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24. Using behaviour to predict stroke severity in conscious rats: post-stroke treatment with 3', 4'-dihydroxyflavonol improves recovery.
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Roulston CL, Callaway JK, Jarrott B, Woodman OL, and Dusting GJ
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- Animals, Brain Mapping, Consciousness, Cytoprotection, Disease Models, Animal, Endothelin-1, Flavonols therapeutic use, Infarction, Middle Cerebral Artery chemically induced, Infarction, Middle Cerebral Artery pathology, Infarction, Middle Cerebral Artery physiopathology, Male, Neuroprotective Agents therapeutic use, Prognosis, Rats, Rats, Wistar, Reproducibility of Results, Severity of Illness Index, Time Factors, Behavior, Animal drug effects, Flavonols pharmacology, Infarction, Middle Cerebral Artery drug therapy, Neuroprotective Agents pharmacology
- Abstract
Prognostic models are used to predict outcome in stroke patients and to stratify treatment groups in clinical trials. No one has previously attempted to use such models in stroke recovery studies in animals. We have now shown the predictive value of assigning stroke severity ratings, based on behaviours displayed in conscious rats during infusion of endothelin-1 to constrict the middle cerebral artery, on neurological and histological outcomes. The validity of prior stratification of treatment groups according to stroke ratings was tested by assessment of the protective potential of synthetic flavonol, 3',4'-dihydroxyflavonol (DiOHF). Neurological deficits and performance on the sticky label test were evaluated before and at 24, 48 and 72 h post-stroke. Histopathology was assessed at 72 h. Positive correlations between stroke ratings and neurological deficit scores were found at 24 (r=0.58, P<0.001), 48 (r=0.53, P<0.001) and 72 (r=0.56, P<0.001) h post-stroke, with more severe strokes associated with worse deficit scores. Similar correlations were observed with the sticky label test. Higher stroke ratings also correlated with greater infarct volumes (total infarct volume: r=0.74, P<0.0001). Treatment with DiOHF (10 mg/kg i.v. given 3, 24 and 48 h post-stroke) significantly reduced infarct volume and restored neurological function in rats with modest stroke ratings (P<0.01), but not in rats with high stroke ratings. These results suggest that stroke ratings, based on behavioural assessment as the stroke develops, reliably predict histopathological and functional outcomes and allow stratification of treatment groups. DiOHF given after stroke improves outcomes in moderate strokes, and therefore has cytoprotective potential.
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- 2008
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25. Pharmacokinetics and brain uptake of AM-36, a novel neuroprotective agent, following intravenous administration to rats.
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Nicolazzo JA, Nguyen TT, Katneni K, Steuten JA, Smith G, Jarrott B, Callaway JK, and Charman SA
- Subjects
- Animals, Area Under Curve, Dose-Response Relationship, Drug, Half-Life, Injections, Intravenous, Male, Neuroprotective Agents administration & dosage, Piperazines administration & dosage, Protein Binding, Rats, Rats, Sprague-Dawley, Time Factors, Tissue Distribution, Blood-Brain Barrier metabolism, Brain metabolism, Neuroprotective Agents pharmacokinetics, Piperazines pharmacokinetics
- Abstract
The plasma pharmacokinetics and brain uptake of the novel neuroprotective agent AM-36 (1-(2-(4-chlorophenyl)-2-hydroxy)ethyl-4-(3,5-bis-(1,1dimethylethyl)-4-hydroxyphenyl) methylpiperazine) were assessed over 72 h following i.v. administration to male Sprague-Dawley rats. At nominal i.v. doses of 0.2, 1 and 3mg kg(-1), AM-36 exhibited an extremely large volume of distribution (18.2-24.6 L kg(-1)) and a long terminal elimination half-life, ranging from 25.2 to 37.7 h. Over this dose range, AM-36 exhibited linear pharmacokinetics, with no apparent change in clearance, volume of distribution or dose-normalised area under the plasma concentration - time curve. AM-36 was very highly bound to plasma proteins (> 99.6%); however, this did not appear to affect the ability of AM-36 to permeate the blood-brain barrier. Following a single i.v. dose of AM-36 at 3mg kg(-1) to rats, brain concentrations were detected for up to 72 h, and the brain-to-plasma ratios were high at all time points (ranging from 8.2 at 5 min post-dose to 0.9 at 72 h post-dose). The very high brain uptake of AM-36 supports previous in-vivo efficacy studies demonstrating the neuroprotective effects of this compound when administered to rats with middle cerebral artery occlusion.
- Published
- 2008
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26. Transient neuroprotection by minocycline following traumatic brain injury is associated with attenuated microglial activation but no changes in cell apoptosis or neutrophil infiltration.
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Bye N, Habgood MD, Callaway JK, Malakooti N, Potter A, Kossmann T, and Morganti-Kossmann MC
- Subjects
- Animals, Brain drug effects, Brain pathology, Brain physiopathology, Brain Injuries metabolism, Brain Injuries pathology, Cell Count, Cerebral Cortex metabolism, Cerebral Cortex pathology, Head Injuries, Closed physiopathology, Interleukin-1beta metabolism, Macrophages pathology, Male, Mice, Mice, Inbred C57BL, Microglia pathology, Motor Activity drug effects, Nervous System drug effects, Nervous System physiopathology, Neutrophils pathology, Time Factors, Apoptosis drug effects, Brain Injuries physiopathology, Microglia drug effects, Minocycline pharmacology, Neuroprotective Agents pharmacology, Neutrophil Infiltration drug effects
- Abstract
Cerebral inflammation and apoptotic cell death are two processes implicated in the progressive tissue damage that occurs following traumatic brain injury (TBI), and strategies to inhibit one or both of these pathways are being investigated as potential therapies for TBI patients. The tetracycline derivative minocycline was therapeutically effective in various models of central nervous system injury and disease, via mechanisms involving suppression of inflammation and apoptosis. We therefore investigated the effect of minocycline in TBI using a closed head injury model. Following TBI, mice were treated with minocycline or vehicle, and the effect on neurological outcome, lesion volume, inflammation and apoptosis was evaluated for up to 7 days. Our results show that while minocycline decreases lesion volume and improves neurological outcome at 1 day post-trauma, this response is not maintained at 4 days. The early beneficial effect is likely not due to anti-apoptotic mechanisms, as the density of apoptotic cells is not affected at either time-point. However, protection by minocycline is associated with a selective anti-inflammatory response, in that microglial activation and interleukin-1beta expression are reduced, while neutrophil infiltration and expression of multiple cytokines are not affected. These findings demonstrate that further studies on minocycline in TBI are necessary in order to consider it as a novel therapy for brain-injured patients.
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- 2007
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27. Inflammatory cell infiltration after endothelin-1-induced cerebral ischemia: histochemical and myeloperoxidase correlation with temporal changes in brain injury.
- Author
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Weston RM, Jones NM, Jarrott B, and Callaway JK
- Subjects
- Animals, Fluorescent Antibody Technique, Immunohistochemistry, Infarction, Middle Cerebral Artery pathology, Macrophages physiology, Male, Microscopy, Confocal, Middle Cerebral Artery physiology, Rats, Rats, Long-Evans, Stereotaxic Techniques, Brain Ischemia chemically induced, Brain Ischemia pathology, Endothelin-1, Inflammation pathology, Neutrophil Infiltration physiology, Peroxidase metabolism
- Abstract
Accumulation of neutrophils in brain after transient focal stroke remains controversial with some studies showing neutrophils to be deleterious, whereas others suggest neutrophils do not contribute to ischemic injury. Myeloperoxidase (MPO) has been used extensively as a marker for quantifying neutrophil accumulation, but is an indirect method and does not detect neutrophils alone. To elucidate the interaction of macrophages in the neutrophil inflammatory response, we conducted double-label immunofluorescence in brain sections at 0, 1, 2, 3, 7, and 15 days after ischemia. Each of these results was obtained from the same animal to determine correlations between neutrophil infiltration and ischemic damage. It was found that MPO activity increased up to 3 days after cerebral ischemia. Dual-staining revealed that macrophages engulf neutrophils in the brain and that this engulfment of neutrophils increased with time, with 50% of neutrophils in the brain engulfed at 3 days and approximately 85% at 15 days (N=5, P<0.05). Interestingly, at 7 days the amount of dual-staining was decreased to 20% (N=5, P<0.05). Neutrophil infiltration was positively correlated with ischemic damage in both the cortex and striatum (r(2)=0.86 and 0.80, respectively, P<0.01). The results of this study indicate that the MPO from neutrophils phagocytized by macrophages may continue to contribute to the overall MPO activity, and that previous assessments that have utilized this marker to measure neutrophil accumulation may have mis-calculated the number of neutrophils within the ischemic territory and hence their contribution to the evolution of the infarct at later time points. Thus any biphasic infiltration of neutrophils may have been masked by the accumulation of macrophages.
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- 2007
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28. AM-36 modulates the neutrophil inflammatory response and reduces breakdown of the blood brain barrier after endothelin-1 induced focal brain ischaemia.
- Author
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Weston RM, Jarrott B, Ishizuka Y, and Callaway JK
- Subjects
- Animals, Brain Ischemia chemically induced, Brain Ischemia pathology, Fluorescent Antibody Technique, Male, Microscopy, Confocal, Peroxidase metabolism, Rats, Rats, Long-Evans, Blood-Brain Barrier drug effects, Brain Ischemia physiopathology, Endothelin-1 pharmacology, Inflammation prevention & control, Neutrophils drug effects, Piperazines pharmacology
- Abstract
Background and Purpose: Following transient focal stroke, rapid accumulation and activation of neutrophils in the ischaemic region is deleterious due to release of reactive oxygen species and myeloperoxidase (MPO). The purpose of this study was to examine whether AM-36, both a Na+ channel blocker and an antioxidant, afforded neuroprotection by modulating neutrophil accumulation into brain, following endothelin-1 (ET-1) induced middle cerebral artery occlusion (MCAo) in conscious rats., Experimental Approach: AM-36 was administered at 3 and 24 h after ET-1-induced MCAo. Functional recovery was determined using grid-walking and cylinder tests. Image analysis of brain sections was used to determine infarct volume. The effect of AM-36 on neutrophil infiltration and their interaction with macrophages was examined in rats at 48 h following MCAo by both an MPO assay and double-label immunofluorescence. Blood brain barrier (BBB) breakdown was measured by the area stained by intravenous Evans Blue., Key Results: AM-36 reduced functional deficits in both tests such that no difference existed from pre-ischaemic values at 48 h. Neutrophil infiltration, assessed by MPO activity, and infarct volume were significantly reduced following AM-36 administration by 54 and 60% respectively. Similarly, immunofluorescence revealed that AM-36 reduced neutrophil infiltration by approximately 50% in selected brain regions, when compared to controls, and also modulated macrophage phagocytosis of neutrophils. Breakdown of the BBB was significantly reduced by 60% following AM-36 treatment., Conclusions and Implications: These findings suggest that AM-36 can directly modulate the neutrophil inflammatory response and reduce BBB breakdown following MCAo.
- Published
- 2006
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29. Injury to axons and oligodendrocytes following endothelin-1-induced middle cerebral artery occlusion in conscious rats.
- Author
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Gresle MM, Jarrott B, Jones NM, and Callaway JK
- Subjects
- Amyloid beta-Protein Precursor metabolism, Animals, Antibodies, Monoclonal metabolism, Brain metabolism, Brain pathology, Cell Count methods, Diffuse Axonal Injury etiology, Disease Models, Animal, Immunohistochemistry methods, Infarction, Middle Cerebral Artery pathology, Infarction, Middle Cerebral Artery physiopathology, Male, Neurofilament Proteins metabolism, Rats, Rats, Long-Evans, Time Factors, Diffuse Axonal Injury pathology, Endothelin-1, Infarction, Middle Cerebral Artery chemically induced, Oligodendroglia pathology
- Abstract
Injury to axons and oligodendrocytes has been poorly characterized in most animal models of stroke, and hence has been difficult to target therapeutically. It is therefore necessary to characterize axonal and oligodendroglial injury in these models, in order to rationally design putative protective compounds that minimize this injury. This study aims to characterize injury to axons and oligodendrocytes in the endothelin-1 (ET-1) model of middle cerebral artery occlusion (MCAO) in conscious rats. Transient forebrain ischemia was induced in conscious adult male Long Evans rats by the perivascular microinjection of ET-1. Quantitative histopathology was performed on forebrain sections at 6, 24, 48 and 72 h after ET-1 administration, using ballistic light analyses and immunohistochemistry for amyloid precursor protein (APP), SMI32, and Tau-1. Ballistic light analyses of cortical and striatal lesions revealed that the infarct volume was maximal in these regions by 6 h. APP and SMI32 immunohistochemistry demonstrated that axonal injury was maximal by 6 h in this model; however, some injured axons appeared to maintain good structural integrity up to 72 h after insult. Density measurements for Tau-1-immunopositive oligodendrocytes were significantly elevated within the corpus callosum from 48 h, but reductions in total oligodendrocyte numbers were not apparent up 72 h after ET-1 injection. These results indicate that axonal and oligodendroglial injury should be investigated as potential targets for delayed therapeutic intervention after MCAO.
- Published
- 2006
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30. Effects of lipopolysaccharide on glial phenotype and activity of glutamate transporters: Evidence for delayed up-regulation and redistribution of GLT-1.
- Author
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O'Shea RD, Lau CL, Farso MC, Diwakarla S, Zagami CJ, Svendsen BB, Feeney SJ, Callaway JK, Jones NM, Pow DV, Danbolt NC, Jarrott B, and Beart PM
- Subjects
- Actins metabolism, Animals, Animals, Newborn, Aspartic Acid metabolism, Astrocytes drug effects, Astrocytes ultrastructure, Biotinylation, Blotting, Western, Cells, Cultured, Cytoskeleton drug effects, Cytoskeleton metabolism, Cytoskeleton ultrastructure, Excitatory Amino Acid Transporter 1 biosynthesis, Excitatory Amino Acid Transporter 2 biosynthesis, Immunohistochemistry, Mice, Mice, Inbred C57BL, Microglia metabolism, Microscopy, Confocal, Neuroglia cytology, Neuroglia metabolism, Phenotype, Protein Transport, Up-Regulation, Excitatory Amino Acid Transporter 2 metabolism, Lipopolysaccharides pharmacology, Neuroglia drug effects
- Abstract
Excitatory amino acid transporters (EAATs) are responsible for homeostasis of extracellular L-glutamate, and the glial transporters are functionally dominant. EAAT expression or function is altered in acute and chronic neurological conditions, but little is known about the regulation of EAATs in reactive astroglia found in such neuropathologies. These studies examined the effects of the bacterial endotoxin lipopolysaccharide (LPS) on glial EAATs in vitro. The effects of LPS (1 microg/ml, 24-72 h) on EAAT activity and expression were examined in primary cultures of mouse astrocytes. [(3)H]D-aspartate uptake increased to 129% of control by 72 h treatment with LPS. Saturation analysis revealed that apparent K(m) was unchanged whilst V(max) was significantly increased to 172% of control by 72 h LPS treatment. Biotinylation and Western blotting indicated that cell-surface expression of GLT-1 was significantly elevated (146% control) by LPS treatment whereas GLAST expression was unchanged. Confocal analyses revealed that LPS treatment resulted in cytoskeletal changes and stellation of astrocytes, with rearrangement of F-actin (as shown by phalloidin labelling). Immunocytochemistry revealed clustering of GLAST, and increased expression and redistribution of GLT-1 to the cell-surface following treatment with LPS. Similar experiments were conducted in microglia, where LPS (50 ng/ml) was found to up-regulate expression of GLT-1 at 24 and 72 h in concert with cytoskeletal changes accompanying activation. These findings suggest an association of cytoskeletal changes in glia with EAAT activity, with the predominant adaptation involving up-regulation and redistribution of GLT-1.
- Published
- 2006
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31. Sodium channel blocking activity of AM-36 and sipatrigine (BW619C89): in vitro and in vivo evidence.
- Author
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Callaway JK, Castillo-Melendez M, Giardina SF, Krstew EK, Beart PM, and Jarrott B
- Subjects
- Animals, Batrachotoxins pharmacology, Guinea Pigs, Male, Mice, Neuroprotective Agents pharmacology, Rats, Rats, Sprague-Dawley, Receptors, Cell Surface antagonists & inhibitors, Receptors, Cell Surface physiology, Receptors, Neurotransmitter antagonists & inhibitors, Receptors, Neurotransmitter physiology, Sodium Channels drug effects, Synaptosomes drug effects, Piperazines pharmacology, Pyrimidines pharmacology, Sodium Channel Blockers pharmacology, Sodium Channels physiology, Synaptosomes physiology
- Abstract
Sodium channel blockers are neuroprotective against cerebral ischemia in animal models. A novel neuroprotective compound AM-36, when screened for activity at the most common receptor and ion channel binding sites, revealed activity at site 2 Na+ channels. Studies then investigated this Na+ channel blocking activity in vitro and in vivo relative to other Na+ channel blockers, including the neuroprotective agent sipatrigine (BW619C89). AM-36 inhibited batrachotoxinin (BTX)-sensitive Na+ channel binding in rat brain homogenates with an IC50 of 0.28 microM. Veratridine (100 microM)-induced neurotoxicity in murine cerebellar granule cells was completely inhibited by AM-36 (1.7 microM) compared to only partial inhibition by sipatrigine (26 microM). Veratridine-stimulated glutamate release, as measured through a microdialysis probe in the cortex of anesthetised rats, was inhibited by 90% by superfusion of AM-36 (1000 microM). In the endothelin-1 (ET-1) model of middle cerebral artery occlusion (MCAo) in conscious rats, both AM-36 (6 mg/kg i.p.) and sipatrigine (10 mg/kg i.p.) 30 min post-MCAo significantly reduced cortical, but not striatal infarct volume. As the refractiveness of the striatum is likely to be dependent on the route and time of drug administration, AM-36 (1 mg/kg i.v.) was administered 3 or 5 h after MCAo and significantly reduced both cortical and striatal infarct volumes. The present studies demonstrate Na+ channel blocking activity of AM-36 both in vitro and in vivo, together with significant neuroprotection when administration is delayed up to 5 h following experimental stroke.
- Published
- 2004
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32. AM-36, a novel neuroprotective agent, profoundly reduces reactive oxygen species formation and dopamine release in the striatum of conscious rats after endothelin-1-induced middle cerebral artery occlusion.
- Author
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Callaway JK, Lawrence AJ, and Jarrott B
- Subjects
- Animals, Brain pathology, Dose-Response Relationship, Drug, Hydroxyl Radical metabolism, Infarction, Middle Cerebral Artery chemically induced, Infarction, Middle Cerebral Artery metabolism, Infarction, Middle Cerebral Artery pathology, Injections, Intraperitoneal, Male, Microdialysis, Neuroprotective Agents administration & dosage, Piperazines administration & dosage, Rats, Rats, Long-Evans, Time Factors, Treatment Outcome, Arterial Occlusive Diseases chemically induced, Corpus Striatum metabolism, Dopamine metabolism, Endothelin-1, Infarction, Middle Cerebral Artery drug therapy, Neuroprotective Agents therapeutic use, Piperazines therapeutic use, Reactive Oxygen Species metabolism
- Abstract
Elevated generation of reactive oxygen species (ROS) has been demonstrated during ischemia and reperfusion. Dopamine (DA) autooxidation may contribute to increased ROS generation. The novel neuroprotective agent AM-36 has antioxidant and Na(+) channel blocking activity and reduces neuronal damage in both cortex and striatum after middle cerebral artery (MCA) occlusion. Here we sought in vivo evidence of the ability of AM-36 to inhibit intrastriatal ROS generation and DA release after ischemia. Salicylate hydroxylation coupled with in vivo microdialysis in the striatum of conscious Long Evans rats was performed during MCA occlusion by perivascular microinjection of endothelin-1 (ET-1). AM-36 (6 mg/kg) was administered intraperitoneally 30 min after MCA occlusion. Dialysates were analysed using high performance liquid chromatography with electrochemical detection for the salicylate hydroxylation product, 2,3-dihydroxybenzoic acid (2,3 DHBA) and for DA and metabolites. MCA occlusion resulted in a marked increase in 2,3 DHBA and a secondary increase in all analytes, 180-300 min later. Increased DA release coincided with 2,3 DHBA formation. AM-36 significantly reduced ischemia induced increases in 2,3 DHBA and DA, and infarct volume in the striatum. Significant improvements in a battery of behavioural tests was also found in AM-36 treated rats. This study has demonstrated profound inhibition of ROS generation by a novel compound with antioxidant activity, administered post-ischemia in conscious rats.
- Published
- 2003
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33. Adolescent Kawasaki disease with uveitis.
- Author
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Callaway LK and Bennett CJ
- Subjects
- Adolescent, Aspirin administration & dosage, Drug Therapy, Combination, Follow-Up Studies, Humans, Male, Mucocutaneous Lymph Node Syndrome drug therapy, Severity of Illness Index, Treatment Outcome, Uveitis drug therapy, Immunoglobulins, Intravenous administration & dosage, Mucocutaneous Lymph Node Syndrome complications, Mucocutaneous Lymph Node Syndrome diagnosis, Uveitis complications, Uveitis diagnosis
- Published
- 2002
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34. Neuroprotective effects of mild hyperthermia prior to focal ischemia in conscious rats.
- Author
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Krstew EV, Jarrott B, and Callaway JK
- Subjects
- Adaptation, Physiological, Animals, Body Temperature, Brain Ischemia pathology, Cerebral Cortex pathology, Cerebral Infarction chemically induced, Cerebral Infarction pathology, Cerebral Infarction physiopathology, Corpus Striatum pathology, Endothelin-1, Male, Motor Activity, Rats, Rats, Wistar, Restraint, Physical, Stroke chemically induced, Stroke pathology, Brain Ischemia physiopathology, Hyperthermia, Induced
- Abstract
Hyperthermia during or after stroke is known to worsen neuronal damage. Paradoxically, when hyperthermia precedes stroke, it can protect against a subsequent ischemic insult. Other stressors including restraint also have a similar pre-conditioning effect. In the present study, we report the unanticipated finding that conscious rats, restrained for the purpose of intravenous infusion, had markedly reduced neuronal and functional deficits after middle cerebral artery occlusion compared with unrestrained rats. Restrained rats had significantly higher body temperature prior to stroke than unrestrained rats. The findings suggest restraint leading to mild hyperthermia may be sufficient to induce adaptive processes which protect against subsequent ischemia.
- Published
- 2001
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35. Incorporation of sodium channel blocking and free radical scavenging activities into a single drug, AM-36, results in profound inhibition of neuronal apoptosis.
- Author
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Callaway JK, Beart PM, Jarrott B, and Giardina SF
- Subjects
- Animals, Cell Survival drug effects, Cells, Cultured, DNA Fragmentation drug effects, Depression, Chemical, Dibucaine pharmacology, Electrophoresis, Agar Gel, Immunohistochemistry, In Situ Nick-End Labeling, Mice, Sodium Channel Agonists, Tetrodotoxin pharmacology, Veratridine pharmacology, Apoptosis drug effects, Free Radical Scavengers pharmacology, Neurons drug effects, Neuroprotective Agents pharmacology, Piperazines pharmacology, Sodium Channel Blockers
- Abstract
AM-36 is a novel neuroprotective agent incorporating both antioxidant and Na(+) channel blocking actions. In cerebral ischaemia, loss of cellular ion homeostasis due to Na(+) channel activation, together with increased reactive oxygen species (ROS) production, are thought to contribute to neuronal death. Since neuronal death in the penumbra of the ischaemic lesion is suggested to occur by apoptosis, we investigated the ability of AM-36, antioxidants and Na(+) channel antagonists to inhibit toxicity induced by the neurotoxin, veratridine in cultured cerebellar granule cells (CGC's). Veratridine (10 - 300 microM) concentration-dependently reduced cell viability of cultured CGC's. Under the experimental conditions employed, cell death induced by veratridine (100 microM) possessed the characteristics of apoptosis as assessed by morphology, TUNEL staining and DNA laddering on agarose gels. Neurotoxicity and apoptosis induced by veratridine (100 microM) were inhibited to a maximum of 50% by the antioxidants, U74500A (0.1 - 10 microM) and U83836E (0.03 - 10 microM), and to a maximum of 30% by the Na(+) channel blocker, dibucaine (0.1 - 100 microM). In contrast, AM-36 (0.01 - 10 microM) completely inhibited veratridine-induced toxicity ( IC(50) 1.7 (1.5 - 1.9) microM, 95% confidence intervals (CI) in parentheses) and concentration-dependently inhibited apoptosis. These findings suggest veratridine-induced toxicity and apoptosis are partially mediated by generation of ROS. AM-36, which combines both Na(+) channel blocking and antioxidant activity, provided superior neuroprotection compared with agents possessing only one of these actions. This bifunctional profile of activity may underlie the potent neuroprotective effects of AM-36 recently found in a stroke model in conscious rats.
- Published
- 2001
- Full Text
- View/download PDF
36. A novel, rapid, computerized method for quantitation of neuronal damage in a rat model of stroke.
- Author
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Callaway JK, Knight MJ, Watkins DJ, Beart PM, Jarrott B, and Delaney PM
- Subjects
- Animals, Brain Ischemia drug therapy, Brain Ischemia physiopathology, Cerebral Cortex drug effects, Cerebral Cortex pathology, Cerebral Cortex physiopathology, Disease Models, Animal, Image Processing, Computer-Assisted, Male, Microscopy, Video methods, Neostriatum drug effects, Neostriatum pathology, Neostriatum physiopathology, Nerve Degeneration drug therapy, Nerve Degeneration physiopathology, Neuroprotective Agents pharmacology, Piperazines pharmacology, Rats, Rats, Wistar, Stroke drug therapy, Stroke physiopathology, Time Factors, Brain Ischemia pathology, Microscopy, Video instrumentation, Nerve Degeneration pathology, Stroke pathology
- Abstract
Determination of extent of infarction in animal models of cerebral ischemia is most commonly achieved by either classical histology (thionin staining) and light microscopy or staining with 2,3, 5-triphenyltetrazolium chloride (TTC). These techniques have limitations and we now describe a novel technique and its validation for assessment of the neuroprotective activity of AM-36, a novel arylalkypiperazine compound with combined antioxidant and sodium channel blocking activity. AM-36 (1.8 mg/kg i.p.) or vehicle, was administered 30 min, 24 and 48 h after endothelin-1-induced middle cerebral artery occlusion in conscious rats. Rats were killed at 72 h, brains removed and frozen in liquid nitrogen prior to coronal sectioning. Using a simple apparatus relying on basic principles of light propagation and a computerised image analysis system, ischemic damage in unstained slide-mounted sections was clearly visualised and measured. AM-36 significantly reduced the area of infarct in both cortex and striatum. The method was verified by thionin staining, and light microscopy. Linear regression analysis showed a highly significant correlation between methods at 72 h for infarct area in the cortex and striatum. Highly significant correlations between methods were found at 3 and 24 h after ischemia. Our method quickly and clearly delineates areas of damage in a manner superior to conventional staining methods.
- Published
- 2000
- Full Text
- View/download PDF
37. Delayed treatment with AM-36, a novel neuroprotective agent, reduces neuronal damage after endothelin-1-induced middle cerebral artery occlusion in conscious rats.
- Author
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Callaway JK, Knight MJ, Watkins DJ, Beart PM, and Jarrott B
- Subjects
- Animals, Cerebral Cortex drug effects, Drug Administration Schedule, Drug Evaluation, Preclinical, Endothelin-1, Infarction, Middle Cerebral Artery chemically induced, Infarction, Middle Cerebral Artery pathology, Injections, Intraperitoneal, Male, Motor Activity drug effects, Neurons drug effects, Neuroprotective Agents administration & dosage, Rats, Rats, Wistar, Treatment Outcome, Infarction, Middle Cerebral Artery drug therapy, Neuroprotective Agents therapeutic use, Piperazines therapeutic use
- Abstract
Background and Purpose: AM-36 is a novel arylalkylpiperazine with combined antioxidant and Na(+) channel blocking actions. Individually, these properties have been shown to confer neuroprotection in a variety of in vitro and in vivo animal models of stroke. Preliminary studies have shown that AM-36 is neuroprotective in vivo. The purpose of the present study was to assess the neuroprotective and behavioral outcome after delayed administration of AM-36 in an endothelin-1-induced, middle cerebral artery model of cerebral ischemia in conscious rats., Methods: Conscious male hooded Wistar rats were subjected to middle cerebral artery occlusion by perivascular microinjection of endothelin-1 via a previously implanted cannula. AM-36 (6 mg/kg IP) or vehicle was administered intraperitoneally 30, 60, or 180 minutes after middle cerebral artery occlusion. Functional outcome was determined 24, 48, and 72 hours after stroke by neurological deficit score, motor performance, and sensory hemineglect tests. Rats were killed at 72 hours, and infarct area and volume were determined by histology and computerized image analysis., Results: Endothelin-1-induced middle cerebral artery occlusion resulted in marked functional deficits and neuronal damage. AM-36 significantly reduced cortical damage when administration was delayed until 30, 60, or 180 minutes after stroke. Interestingly, neuronal damage was time-dependently reduced, with the greatest protection found when AM-36 was administered 180 minutes after stroke. Striatal damage was significantly reduced after treatment with AM-36 at 180 minutes after stroke. Functional outcome paralleled histopathology. Rota-rod performance, sensory hemineglect, and neurological deficit scores returned to preischemia levels in AM-36-treated rats by 72 hours after stroke when administration was delayed by 180 minutes after stroke., Conclusions: AM-36 potently protects against both neuronal damage and functional deficits even when administered up to 180 minutes after induction of stroke. In fact, the greatest protection was found when administration was delayed by 180 minutes after stroke. The possible mechanisms of action of AM-36 are discussed. The present findings suggest that AM-36 may have great promise in the acute treatment of human stroke.
- Published
- 1999
- Full Text
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38. A reliable procedure for comparison of antioxidants in rat brain homogenates.
- Author
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Callaway JK, Beart PM, and Jarrott B
- Subjects
- Animals, Brain metabolism, Brain Chemistry, Chromans pharmacology, Deferoxamine pharmacology, Ferric Compounds pharmacology, Inhibitory Concentration 50, Iron Chelating Agents pharmacology, Lipid Peroxidation drug effects, Male, Piperazines pharmacology, Rats, Rats, Sprague-Dawley, Sensitivity and Specificity, Thiobarbituric Acid Reactive Substances metabolism, Antioxidants pharmacology, Brain drug effects
- Abstract
Lipid peroxidation is a major consequence of oxidative stress and an important cause of neuronal damage in ischaemic injuries and neurodegenerative disorders such as Parkinson's disease. Recent research has focused on the development of antioxidant drugs which may delay or minimize neurodegeneration. Rapid and reliable assays are therefore necessary in order to evaluate novel antioxidant compounds. A widely adopted method for measurement of lipid peroxidation is the thiobarbituric acid reacting substances (TBARS) assay. Several variations of this method have appeared in the literature, some of which have been tested by us without success. We have therefore established a reliable procedure which takes into account the most important factors previously found to influence the TBARS method. Briefly, various concentrations of drug were added to rat brain homogenates (10% w/v in 20 mM Tris-HCl buffer, pH 7.4) and incubated at 37 degrees C for 10 min before addition of ammonium ferric sulphate (100 or 1000 microM) and a further incubation at 37 degrees C for 30 min. Proteins were then precipitated with 8.1% sodium dodecyl sulphate, the reaction stopped with 20% acetic acid, and the samples were then centrifuged for 15 min. Aliquots of supernatant were added to an equal volume of thiobarbituric acid (0.8%), samples were heated at 95 degrees C for 30 min, and then cooled on ice before reading at 532 nm. The present adaptation represents a simple and highly reproducible assay which does not require difficult extraction procedures with hazardous chemicals and results in a stable chromagen. The method has been evaluated using a number of structurally distinct antioxidants and iron chelators. IC50 values (microM) for percentage inhibition of TBARS formation were as follows: desferroxamine (1.1), U83836E (1.7), butylated hydroxytoluene (13), U74500A (20), LY231617 (22), idebenone (89), and Trolox (110). This order of potency was comparable to that found with a commercially available, but expensive kit designed to specifically measure malondialdehyde (Spearman's rank correlation coefficient, p < 0.01).
- Published
- 1998
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39. Effects of inhaled alpha 2-adrenoceptor and GABAB receptor agonists on citric acid-induced cough and tidal volume changes in guinea pigs.
- Author
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Callaway JK and King RG
- Subjects
- Acetylcholine pharmacology, Administration, Inhalation, Adrenergic alpha-Agonists therapeutic use, Animals, Atropine pharmacology, Azepines administration & dosage, Azepines pharmacology, Baclofen administration & dosage, Baclofen pharmacology, Citric Acid, Cough chemically induced, Guinea Pigs, Male, Receptors, GABA-A drug effects, Respiration drug effects, Xylazine administration & dosage, Xylazine pharmacology, Yohimbine pharmacology, gamma-Aminobutyric Acid administration & dosage, gamma-Aminobutyric Acid pharmacology, Adrenergic alpha-Agonists pharmacology, Citrates toxicity, Cough drug therapy, Receptors, GABA-A metabolism, Tidal Volume drug effects
- Abstract
The effects of alpha 2-adrenoceptor and GABA receptor agonists on citric acid-induced cough and increased tidal volume were investigated in conscious guinea pigs. Inhalation of low doses of B-HT 920 (5-allyl-2-amino 5,6,7,8-tetrahydro-4H-thiazolo[4,5-d]azepine dihydrochloride), and xylazine significantly inhibited citric acid-induced cough and tidal volume increases. Intraperitoneal administration of higher doses of B-HT 920 than those given by aerosol were ineffective. The inhibitory effects of B-HT 920 were antagonised by prior intraperitoneal administration of yohimbine, but not atropine. Inhalation of GABA or baclofen inhibited tidal volume increases, but had no effect on cough. Inhaled alpha 2-adrenoceptor or GABA agonists had no effect on the reduced respiratory rate after citric acid inhalation. It is concluded that alpha 2-adrenoceptor agonists inhibit cough via a mechanism which may not be related to their ability to reduce citric acid-induced tidal volume increases, since GABA and baclofen inhibited tidal volume increases but not cough. We suggest that alpha 2-adrenoceptor agonists may have therapeutic potential in the treatment of cough.
- Published
- 1992
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40. Evidence for peripheral mechanisms mediating the antitussive actions of opioids in the guinea pig.
- Author
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Callaway JK, King RG, and Boura AL
- Subjects
- Administration, Inhalation, Animals, Antitussive Agents administration & dosage, Citrates, Citric Acid, Codeine administration & dosage, Codeine pharmacology, Cough chemically induced, Cough prevention & control, Dextromethorphan administration & dosage, Dextromethorphan pharmacology, Female, Guinea Pigs, Injections, Intraperitoneal, Morphine administration & dosage, Morphine pharmacology, Naloxone pharmacology, Narcotics administration & dosage, Oligopeptides administration & dosage, Oligopeptides pharmacology, Oxymorphone pharmacology, Antitussive Agents pharmacology, Narcotics pharmacology, Peripheral Nerves drug effects
- Abstract
1. Comparisons were made between the doses required of aerosol and intraperitoneally administered morphine, dextromethorphan, codeine and the specific peripherally acting mu-receptor agonist DALDA (H-Tyr-D-Arg-Phe-Lys-NH2) to suppress citric acid-induced coughing in conscious guinea pigs. 2. Estimated ID50s for inhibition of numbers of coughs induced by an aerosol of 5% citric acid were 1.0 and 2.4 mg/kg for intraperitoneally administered morphine and dextromethorphan, respectively. 3. The estimated ID50s after inhalation of morphine and dextromethorphan as aerosols were approximately 2.2 and approximately 12 micrograms/kg, respectively. 4. Aerosilized codeine (approximately 72 micrograms/kg, n = 5) significantly inhibited coughing by 62 +/- 23% whereas 3 mg/kg, i.p. was required to significantly reduce coughing by a similar degree (60 +/- 6%, n = 7). 5. Inhalation of DALDA (approximately 7.2 micrograms/kg, n = 7) also significantly inhibited coughing. 6. The antitussive effect of inhaled morphine (approximately 7.2 micrograms/kg, n = 11) was inhibited after administration of 3 mg/kg of either naloxone hydrochloride or naloxone methylbromide intraperitoneally. 7. The results support the hypothesis that effects at a peripheral site can make a major contribution to the antitussive actions of these drugs.
- Published
- 1991
- Full Text
- View/download PDF
41. Methoxyphenamine inhibits histamine-induced bronchoconstriction in anaesthetized guinea-pigs and histamine-induced contractions of guinea-pig ileum in vitro.
- Author
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Callaway JK, King RG, and Boura AL
- Subjects
- 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid, Acetylcholine pharmacology, Anesthesia, Animals, Guinea Pigs, Histamine pharmacology, Ileum drug effects, In Vitro Techniques, Male, Methamphetamine pharmacology, Prostaglandin Endoperoxides, Synthetic pharmacology, Pyrilamine pharmacology, Adrenergic beta-Agonists pharmacology, Bronchi drug effects, Histamine Antagonists, Methamphetamine analogs & derivatives, Muscle, Smooth drug effects
- Abstract
Measurements were made of the effects of methoxyphenamine hydrochloride on histamine-, acetylcholine- and U46619-induced bronchoconstriction in pentobarbitone-anaesthetized guinea-pigs, and on histamine- and acetylcholine-induced contractions of guinea-pig ileum in vitro. Methoxyphenamine (20 mg/kg, i.v.) did not affect bronchoconstriction induced by acetylcholine or the thromboxane A2-mimetic U46619. However, it produced a parallel rightward shift [2.94-(1.79, 4.41) fold, 95% confidence limits in parentheses] of the curve relating bronchoconstrictor responses to log-dose of histamine at a total dose of 13 mg/kg, i.v., which was not significantly different from the shift [3.30-(1.93, 5.56) fold] produced by 3 micrograms/kg, i.v., of the histamine antagonist mepyramine maleate. Histamine-induced contractions of the isolated guinea-pig ileum were antagonized by methoxyphenamine (10(-5) to 10(-3) M). The histamine log-concentration-response curve was shifted to the right in a parallel manner by methoxyphenamine (10(-5) to 10(-4) M), without depression of maximum responses. However, at higher concentrations, maximum responses were reduced. The slope of the Schild plot was significantly different from -1. The degree of the rightward shift of the concentration-response curves to histamine, produced by 10(-5) M of methoxyphenamine [3.90-(2.83, 4.97) fold], was not significantly different from that produced by 3 x 10(-9) M of mepyramine [4.60-(2.86, 6.52) fold]. Methoxyphenamine, at concentrations of 10(-5) to 3 x 10(-4) M, had no significant effect on responses of guinea-pig ilea to acetylcholine (10(-9) to 10(-5) M). These results indicate that methoxyphenamine antagonizes the effects of histamine both in vivo and in vitro. In vitro studies indicate a noncompetitive antagonism.
- Published
- 1990
42. Myocardial protection for the compromised ventricle during cardiac surgery: a comparative study.
- Author
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Williams GD, McNair WR, Burnett HF, Osam PN, Flacke J, and Callaway JK
- Subjects
- Aged, Arrhythmias, Cardiac etiology, Cardiac Output, Cardiopulmonary Bypass, Heart Failure etiology, Humans, Hypothermia, Induced, Middle Aged, Myocardial Infarction etiology, Myocardial Revascularization mortality, Perfusion methods, Postoperative Complications etiology, Postoperative Complications mortality, Myocardial Contraction, Myocardial Revascularization methods, Postoperative Complications prevention & control
- Published
- 1976
- Full Text
- View/download PDF
43. Pulsatile perfusion versus conventional high-flow nonpulsatile perfusion for rapid core cooling and rewarming of infants for circulatory arrest in cardiac operation.
- Author
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Williams GD, Seifen AB, Lawson NW, Norton JB, Readinger RI, Dungan TW, Callaway JK, and Campbell GS
- Subjects
- Acid-Base Equilibrium, Age Factors, Blood Cells, Body Temperature, Cardiopulmonary Bypass instrumentation, Catheterization instrumentation, Catheterization methods, Evaluation Studies as Topic, Heart Arrest, Induced instrumentation, Humans, Hypothermia, Induced instrumentation, Infant, Kidney physiology, Postoperative Care, Postoperative Complications prevention & control, Respiration, Artificial, Time Factors, Cardiopulmonary Bypass methods, Heart Arrest, Induced methods, Heart Defects, Congenital surgery, Hypothermia, Induced methods
- Abstract
Thirty consecutive infants undergoing hypothermia and circulatory arrest for repair of ventricular septal defect, transposition of the great vessels, or atrioventricular canal defects were alternately selected for conventional high flow nonpulsatile perfusion or pulsatile perfusion during core cooling and rewarming. All received morphine anesthesia, 30 mg/kg of Solu-Medrol, and 10 to 15 mcg/kg of phentolamine. Those receiving nonpulsatile flow were perfused at a rate of 160 to 180 cc/kg/min with a roller pump and oxygenator with arterial pressure of 50 to 55 mm Hg. In the pulsatile flow group, a roller pump and oxygenator were used, and an especially constructed Datascope PAD (pulsatile assist device) was interposed in the arterial line to provide pulsatile perfusion with 75/40 mm Hg pressure at slightly reduced flow (150 cc/kg/min). The average rectal, esophageal, and tympanic membrane temperatures were reduced to approximately 16 degrees C prior to circulatory arrest. Following repair, perfusion was resumed until these temperatures returned to 37 degrees C. Cooling and rewarming were enhanced by pulsatile perfusion, with over 30% reduction in total pump time. Additionally, the larger patients in the pulsatile group cooled almost as rapidly as the smaller. The rates of decline and subsequent rise of rectal, esophageal, and tympanic membrane temperatures were equal in the pulsatile group, but the rectal temperature lagged far behind in the nonpulsatile group. Urine production during bypass was 100% greater in the pulsatile group. The plasma free hemoglobin was similar in both groups. The average postrewarming pH was 7.31 in the nonpulsatile group and 7.42 in the pulsatile group. Infants receiving pulsatile flow awakened more quickly, were more alert, and required less postoperative mechanical ventilation. We suggest that pulsatile perfusion for core cooling and rewarming of infants is safe and is more rapid and physiological than conventional high-flow nonpulsatile perfusion.
- Published
- 1979
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